AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011171.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011171.01.T01 MTR_2g100060 95.385 65 2 1 1 64 188 252 1.12e-34 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 64 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTAAGATAGGGAATATA+TGG 0.206677 2:-80503172 MsG0280011171.01.T01:intron
GTTAATCCACCTCCGGTATA+AGG 0.234306 2:+80502831 None:intergenic
GAAATACCTAGCTTCTTTAA+AGG 0.246992 2:-80503122 MsG0280011171.01.T01:intron
CTTTAACTAAACAGGTCTTT+TGG 0.264585 2:-80503918 MsG0280011171.01.T01:intron
TTCGAAGTTGACGCCGTTAC+TGG 0.277675 2:+80505038 None:intergenic
TCAACTTTCCAAGGAGGATA+TGG 0.284480 2:-80503354 MsG0280011171.01.T01:CDS
CAACTAGTGAGTGGGGTAGC+TGG 0.328092 2:-80503855 MsG0280011171.01.T01:CDS
AACTAGTGAGTGGGGTAGCT+GGG 0.333096 2:-80503854 MsG0280011171.01.T01:CDS
TTCTATGGTAACAAGAGTTC+GGG 0.341017 2:-80502971 MsG0280011171.01.T01:CDS
ATGTTAAGGAGAACCTTAAA+AGG 0.348838 2:-80503487 MsG0280011171.01.T01:intron
GGGGATGCCTGTGCATGTTG+GGG 0.350547 2:-80503895 MsG0280011171.01.T01:CDS
TCTCATTCTTCAAGAAGTTA+TGG 0.379999 2:-80502933 MsG0280011171.01.T01:CDS
TTTCTATGGTAACAAGAGTT+CGG 0.381266 2:-80502972 MsG0280011171.01.T01:CDS
AGTTGAGATGATTAAGGACT+TGG 0.394600 2:-80503396 MsG0280011171.01.T01:CDS
GATGTAGTGTCTAATTTCTA+TGG 0.394956 2:-80502986 MsG0280011171.01.T01:CDS
TATATGGGTTGAAAGTGTTT+CGG 0.406972 2:-80503156 MsG0280011171.01.T01:CDS
GTTGTTAGTTGAGATGATTA+AGG 0.409976 2:-80503402 MsG0280011171.01.T01:CDS
CTAAAGCCTTTGTTGAATTG+TGG 0.416793 2:-80503325 MsG0280011171.01.T01:CDS
TACAAAAGTGAAGGCTAAAG+TGG 0.436063 2:-80505141 MsG0280011171.01.T01:CDS
GGGATGCCTGTGCATGTTGG+GGG 0.444947 2:-80503894 MsG0280011171.01.T01:CDS
TTAAGTGTGATGTTGATGTT+AGG 0.446188 2:-80505013 MsG0280011171.01.T01:intron
TAAAGCCTTTGTTGAATTGT+GGG 0.446353 2:-80503324 MsG0280011171.01.T01:CDS
TTTAGCGTTGAAAGTGAAGT+TGG 0.454211 2:-80505165 MsG0280011171.01.T01:CDS
TTAATCCACCTCCGGTATAA+GGG 0.464412 2:+80502832 None:intergenic
CCTCAAAAGGTGGGGTAAAG+AGG 0.466759 2:-80503453 MsG0280011171.01.T01:CDS
AGAAGTTATGGCCGCTCTTG+AGG 0.470596 2:-80502921 MsG0280011171.01.T01:CDS
GGCTCATGGGTAACCTCAAA+AGG 0.472785 2:-80503466 MsG0280011171.01.T01:CDS
AGCCAGAGCCATATCCTCCT+TGG 0.474280 2:+80503346 None:intergenic
GAAGTTATGGCCGCTCTTGA+GGG 0.475765 2:-80502920 MsG0280011171.01.T01:CDS
GTAACTCTTATTCCGACGTT+AGG 0.486919 2:+80505104 None:intergenic
TTATTGTATCACCCTTATAC+CGG 0.501481 2:-80502843 MsG0280011171.01.T01:CDS
TATAAGGGTGATACAATAAG+AGG 0.505142 2:+80502847 None:intergenic
TTCCAAGGAGGATATGGCTC+TGG 0.506785 2:-80503348 MsG0280011171.01.T01:CDS
GGAGAACCTTAAAAGGCTCA+TGG 0.512126 2:-80503480 MsG0280011171.01.T01:CDS
AGGTGGGGTAAAGAGGTCTT+TGG 0.513836 2:-80503446 MsG0280011171.01.T01:CDS
ACTGGTTTCTTATTACCGAC+AGG 0.516093 2:+80505056 None:intergenic
TATCACCCTTATACCGGAGG+TGG 0.526867 2:-80502837 MsG0280011171.01.T01:CDS
TTAAATGGTTAATCCACCTC+CGG 0.533012 2:+80502824 None:intergenic
CTCGTGCTCAACTAGTGAGT+GGG 0.533350 2:-80503863 MsG0280011171.01.T01:CDS
TCTTAAGATAGGGAATATAT+GGG 0.554488 2:-80503171 MsG0280011171.01.T01:intron
CTTTGGTAATCTCGACCTCA+TGG 0.554736 2:-80503429 MsG0280011171.01.T01:CDS
GAAATACCTTTAAAGAAGCT+AGG 0.568293 2:+80503116 None:intergenic
GAGAACCTTAAAAGGCTCAT+GGG 0.572883 2:-80503479 MsG0280011171.01.T01:CDS
CATGGGTAACCTCAAAAGGT+GGG 0.581714 2:-80503462 MsG0280011171.01.T01:CDS
AGAAGCGATTCAACTTTCCA+AGG 0.583431 2:-80503363 MsG0280011171.01.T01:CDS
GAAGTTGGATACAAAAGTGA+AGG 0.584664 2:-80505150 MsG0280011171.01.T01:CDS
GTTAGAGTTCATGTTCCTGT+CGG 0.590463 2:-80505071 MsG0280011171.01.T01:CDS
TCATGGGTAACCTCAAAAGG+TGG 0.600952 2:-80503463 MsG0280011171.01.T01:CDS
ACTAGTGAGTGGGGTAGCTG+GGG 0.614901 2:-80503853 MsG0280011171.01.T01:intron
ACAAAAGTGAAGGCTAAAGT+GGG 0.615350 2:-80505140 MsG0280011171.01.T01:CDS
TCTCGTGCTCAACTAGTGAG+TGG 0.618224 2:-80503864 MsG0280011171.01.T01:CDS
ATGGGTTGAAAGTGTTTCGG+AGG 0.620545 2:-80503153 MsG0280011171.01.T01:CDS
AGAAGCTAGGTATTTCATCA+CGG 0.624150 2:+80503129 None:intergenic
TTGTATCACCCTTATACCGG+AGG 0.630873 2:-80502840 MsG0280011171.01.T01:CDS
AAGCACCCACAATTCAACAA+AGG 0.650927 2:+80503319 None:intergenic
GGAATAAGAGTTACATGTGA+CGG 0.651161 2:-80505095 MsG0280011171.01.T01:CDS
TCGTGCTCAACTAGTGAGTG+GGG 0.670145 2:-80503862 MsG0280011171.01.T01:CDS
CGGTAATAAGAAACCAGTAA+CGG 0.676522 2:-80505051 MsG0280011171.01.T01:CDS
ATGGGTAACCTCAAAAGGTG+GGG 0.677533 2:-80503461 MsG0280011171.01.T01:CDS
AAACATCACACCCTCAAGAG+CGG 0.678741 2:+80502910 None:intergenic
AGCGATTCAACTTTCCAAGG+AGG 0.681343 2:-80503360 MsG0280011171.01.T01:CDS
AAAATGAAAACACCTAACGT+CGG 0.681999 2:-80505116 MsG0280011171.01.T01:CDS
AACTTCGTAGAAAATTGACA+TGG 0.690048 2:+80502873 None:intergenic
CAACTAACAACTCTACCATG+AGG 0.727473 2:+80503414 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTAATCTTAAAATTTA+AGG + Chr2:80503625-80503644 None:intergenic 10.0%
!!! TTTGGTATAATATTTTTTTT+TGG - Chr2:80503177-80503196 MsG0280011171.01.T01:intron 10.0%
!! ACTTCATCAATTAAATTAAA+CGG + Chr2:80503046-80503065 None:intergenic 15.0%
!! ATAAATTACTTTAACTAAAC+AGG - Chr2:80504088-80504107 MsG0280011171.01.T01:intron 15.0%
!!! CCTTTTATTTTTAATATCTT+CGG + Chr2:80503481-80503500 None:intergenic 15.0%
!!! TATAATTTTAGAATAGACAA+TGG + Chr2:80504064-80504083 None:intergenic 15.0%
!!! TTGTCTATTCTAAAATTATA+TGG - Chr2:80504064-80504083 MsG0280011171.01.T01:intron 15.0%
!! ATATGTGAAAGAAATTAGAT+TGG + Chr2:80503738-80503757 None:intergenic 20.0%
!! CCGAAGATATTAAAAATAAA+AGG - Chr2:80503478-80503497 MsG0280011171.01.T01:CDS 20.0%
!! TACTAAAAAATCAAGATGAT+TGG + Chr2:80503820-80503839 None:intergenic 20.0%
!! TGTGCTAAATAATGTTAAAT+TGG - Chr2:80503376-80503395 MsG0280011171.01.T01:CDS 20.0%
!! TTAATAAAAAAAGCTGCAAA+TGG + Chr2:80503509-80503528 None:intergenic 20.0%
!! TTAATTAAAGCGAAATTTGT+TGG - Chr2:80503525-80503544 MsG0280011171.01.T01:intron 20.0%
!! TTGTTGAAACAAAATGATTT+CGG + Chr2:80504424-80504443 None:intergenic 20.0%
!! TTTACGGTTGAAAAATTAAT+GGG + Chr2:80503558-80503577 None:intergenic 20.0%
!! TTTCTTTCACATATGATTAA+TGG - Chr2:80503744-80503763 MsG0280011171.01.T01:intron 20.0%
!!! AATATTTTGTTTCTTGCTTT+TGG - Chr2:80503129-80503148 MsG0280011171.01.T01:CDS 20.0%
!!! CATTTTGTTTCAACAATTTT+AGG - Chr2:80504428-80504447 MsG0280011171.01.T01:intron 20.0%
!!! TCTTGATTTTTTAGTAGTAA+AGG - Chr2:80503824-80503843 MsG0280011171.01.T01:intron 20.0%
!!! TTTTACGGTTGAAAAATTAA+TGG + Chr2:80503559-80503578 None:intergenic 20.0%
! AGTAGTAAAGGAAACTAAAT+AGG - Chr2:80503836-80503855 MsG0280011171.01.T01:intron 25.0%
! TACTTATGTATGAATCAAAG+AGG - Chr2:80504799-80504818 MsG0280011171.01.T01:intron 25.0%
! TCAATTTGACTCTTAAGATA+GGG - Chr2:80504833-80504852 MsG0280011171.01.T01:intron 25.0%
! TCTTAAGATAGGGAATATAT+GGG - Chr2:80504843-80504862 MsG0280011171.01.T01:intron 25.0%
! TTCAATTTGACTCTTAAGAT+AGG - Chr2:80504832-80504851 MsG0280011171.01.T01:intron 25.0%
! TTGTCAACATAAAAAACTGA+TGG - Chr2:80503438-80503457 MsG0280011171.01.T01:CDS 25.0%
!! AGATTGCAAAACTTTTGTAT+CGG + Chr2:80504955-80504974 None:intergenic 25.0%
!! TTTAACTAAACAGGTCTTTT+GGG - Chr2:80504097-80504116 MsG0280011171.01.T01:intron 25.0%
!!! CGAGTTTTATATTTTTTGGT+TGG - Chr2:80504494-80504513 MsG0280011171.01.T01:intron 25.0%
!!! TGGACGAGTTTTATATTTTT+TGG - Chr2:80504490-80504509 MsG0280011171.01.T01:intron 25.0%
AAAATGAAAACACCTAACGT+CGG - Chr2:80502898-80502917 MsG0280011171.01.T01:CDS 30.0%
AACTTCGTAGAAAATTGACA+TGG + Chr2:80505144-80505163 None:intergenic 30.0%
AAGGAAAGAGATGCAAATAT+TGG - Chr2:80504769-80504788 MsG0280011171.01.T01:intron 30.0%
AAGGTTCTCCTTAACATTAT+AGG + Chr2:80504524-80504543 None:intergenic 30.0%
ATAAAAAAAGCTGCAAATGG+TGG + Chr2:80503506-80503525 None:intergenic 30.0%
ATGTTAAGGAGAACCTTAAA+AGG - Chr2:80504527-80504546 MsG0280011171.01.T01:intron 30.0%
CAATTGAAGTTATCCTCATT+TGG - Chr2:80503908-80503927 MsG0280011171.01.T01:intron 30.0%
CAGAAACAAGAATACAATCA+AGG + Chr2:80504302-80504321 None:intergenic 30.0%
CTCTTAAGATAGGGAATATA+TGG - Chr2:80504842-80504861 MsG0280011171.01.T01:intron 30.0%
GAAATACCTAGCTTCTTTAA+AGG - Chr2:80504892-80504911 MsG0280011171.01.T01:intron 30.0%
GATGTAGTGTCTAATTTCTA+TGG - Chr2:80505028-80505047 MsG0280011171.01.T01:CDS 30.0%
GGAAACTAAATAGGAATATG+TGG - Chr2:80503845-80503864 MsG0280011171.01.T01:intron 30.0%
GTTGTTAGTTGAGATGATTA+AGG - Chr2:80504612-80504631 MsG0280011171.01.T01:intron 30.0%
TAAAGCCTTTGTTGAATTGT+GGG - Chr2:80504690-80504709 MsG0280011171.01.T01:intron 30.0%
TATAAGGGTGATACAATAAG+AGG + Chr2:80505170-80505189 None:intergenic 30.0%
TCTCATTCTTCAAGAAGTTA+TGG - Chr2:80505081-80505100 MsG0280011171.01.T01:CDS 30.0%
TTAAGTGTGATGTTGATGTT+AGG - Chr2:80503001-80503020 MsG0280011171.01.T01:CDS 30.0%
TTATCTGATACATGCTTTGT+GGG - Chr2:80503993-80504012 MsG0280011171.01.T01:intron 30.0%
TTATTGTATCACCCTTATAC+CGG - Chr2:80505171-80505190 MsG0280011171.01.T01:CDS 30.0%
TTGAGAAAATATGTCCTTGT+CGG - Chr2:80504979-80504998 MsG0280011171.01.T01:intron 30.0%
TTGGATCACCTATAATGTTA+AGG - Chr2:80504513-80504532 MsG0280011171.01.T01:intron 30.0%
TTTATCTGATACATGCTTTG+TGG - Chr2:80503992-80504011 MsG0280011171.01.T01:intron 30.0%
TTTCTATGGTAACAAGAGTT+CGG - Chr2:80505042-80505061 MsG0280011171.01.T01:CDS 30.0%
! CTTTAACTAAACAGGTCTTT+TGG - Chr2:80504096-80504115 MsG0280011171.01.T01:intron 30.0%
! GAAATACCTTTAAAGAAGCT+AGG + Chr2:80504901-80504920 None:intergenic 30.0%
! GTGGAAGAATTTTGTATATC+TGG - Chr2:80503940-80503959 MsG0280011171.01.T01:intron 30.0%
! TTAACTAAACAGGTCTTTTG+GGG - Chr2:80504098-80504117 MsG0280011171.01.T01:intron 30.0%
! TTAGGTTTAAGATCTGGAAA+TGG - Chr2:80503019-80503038 MsG0280011171.01.T01:intron 30.0%
! TTGATGTTAGGTTTAAGATC+TGG - Chr2:80503013-80503032 MsG0280011171.01.T01:intron 30.0%
!! ATGCTCTTTTTCACTTTCTT+TGG - Chr2:80503871-80503890 MsG0280011171.01.T01:CDS 30.0%
!! TATATGGGTTGAAAGTGTTT+CGG - Chr2:80504858-80504877 MsG0280011171.01.T01:intron 30.0%
!! TCTTTAAAGGTATTTCTCGA+AGG - Chr2:80504905-80504924 MsG0280011171.01.T01:intron 30.0%
!! TGATTGTATTCTTGTTTCTG+AGG - Chr2:80504302-80504321 MsG0280011171.01.T01:intron 30.0%
!! TGCTCTTTTTCACTTTCTTT+GGG - Chr2:80503872-80503891 MsG0280011171.01.T01:CDS 30.0%
!! TGTTATTAGCTCTAAACTGA+AGG - Chr2:80503408-80503427 MsG0280011171.01.T01:CDS 30.0%
!! TTAAAGCGAAATTTGTTGGA+TGG - Chr2:80503529-80503548 MsG0280011171.01.T01:intron 30.0%
!!! TTTGAGTGAGACTTGTTTTA+CGG + Chr2:80503574-80503593 None:intergenic 30.0%
AAATGTCGACAATGACTACT+GGG - Chr2:80503238-80503257 MsG0280011171.01.T01:intron 35.0%
AACACATGACAGAATCATAG+AGG - Chr2:80503086-80503105 MsG0280011171.01.T01:intron 35.0%
ACAAAAGTGAAGGCTAAAGT+GGG - Chr2:80502874-80502893 MsG0280011171.01.T01:CDS 35.0%
AGAAGCTAGGTATTTCATCA+CGG + Chr2:80504888-80504907 None:intergenic 35.0%
AGTTGAGATGATTAAGGACT+TGG - Chr2:80504618-80504637 MsG0280011171.01.T01:intron 35.0%
ATGAAGTAAACAAGTCCATG+TGG - Chr2:80503059-80503078 MsG0280011171.01.T01:intron 35.0%
CAACTAATCACACTGTTGTT+AGG + Chr2:80504279-80504298 None:intergenic 35.0%
CGGTAATAAGAAACCAGTAA+CGG - Chr2:80502963-80502982 MsG0280011171.01.T01:CDS 35.0%
CTAAAGCCTTTGTTGAATTG+TGG - Chr2:80504689-80504708 MsG0280011171.01.T01:intron 35.0%
CTTGAAGACCTGATGATTTA+TGG - Chr2:80504038-80504057 MsG0280011171.01.T01:intron 35.0%
GAAGTTGGATACAAAAGTGA+AGG - Chr2:80502864-80502883 MsG0280011171.01.T01:CDS 35.0%
GGAATAAGAGTTACATGTGA+CGG - Chr2:80502919-80502938 MsG0280011171.01.T01:CDS 35.0%
TAAATGTCGACAATGACTAC+TGG - Chr2:80503237-80503256 MsG0280011171.01.T01:intron 35.0%
TACAAAAGTGAAGGCTAAAG+TGG - Chr2:80502873-80502892 MsG0280011171.01.T01:CDS 35.0%
TATCCTCATTTGGTTAGTAG+TGG - Chr2:80503918-80503937 MsG0280011171.01.T01:intron 35.0%
TATCTGATACATGCTTTGTG+GGG - Chr2:80503994-80504013 MsG0280011171.01.T01:intron 35.0%
TGAAGTAAACAAGTCCATGT+GGG - Chr2:80503060-80503079 MsG0280011171.01.T01:intron 35.0%
TGAATCTCTTCAGAAAGAAC+TGG + Chr2:80505007-80505026 None:intergenic 35.0%
TTAGGTATTCTTTGCAGTTG+TGG - Chr2:80504395-80504414 MsG0280011171.01.T01:intron 35.0%
TTCAAAGATCTTGATCCATG+TGG - Chr2:80504743-80504762 MsG0280011171.01.T01:intron 35.0%
TTCTATGGTAACAAGAGTTC+GGG - Chr2:80505043-80505062 MsG0280011171.01.T01:CDS 35.0%
! TAACTAAACAGGTCTTTTGG+GGG - Chr2:80504099-80504118 MsG0280011171.01.T01:intron 35.0%
! TTTAGCGTTGAAAGTGAAGT+TGG - Chr2:80502849-80502868 MsG0280011171.01.T01:CDS 35.0%
!! AATTTTAGGCTTGCTCATTG+TGG - Chr2:80504442-80504461 MsG0280011171.01.T01:intron 35.0%
!! ATCTCCAATCTTTAAAGTGC+GGG - Chr2:80503782-80503801 MsG0280011171.01.T01:intron 35.0%
!! CATCTCCAATCTTTAAAGTG+CGG - Chr2:80503781-80503800 MsG0280011171.01.T01:intron 35.0%
!! GCTCTTTTTCACTTTCTTTG+GGG - Chr2:80503873-80503892 MsG0280011171.01.T01:CDS 35.0%
AAGCACCCACAATTCAACAA+AGG + Chr2:80504698-80504717 None:intergenic 40.0%
AATGTCGACAATGACTACTG+GGG - Chr2:80503239-80503258 MsG0280011171.01.T01:intron 40.0%
ACAAAGCATGTCCCTAACAA+GGG + Chr2:80504246-80504265 None:intergenic 40.0%
AGAAGCGATTCAACTTTCCA+AGG - Chr2:80504651-80504670 MsG0280011171.01.T01:intron 40.0%
ATCTCTTTCCTTCAACCACA+TGG + Chr2:80504761-80504780 None:intergenic 40.0%
ATCTGATACATGCTTTGTGG+GGG - Chr2:80503995-80504014 MsG0280011171.01.T01:intron 40.0%
ATCTTGATCCATGTGGTTGA+AGG - Chr2:80504750-80504769 MsG0280011171.01.T01:intron 40.0%
CAAAGAATACCTAAGCACCA+GGG + Chr2:80504389-80504408 None:intergenic 40.0%
CAACTAACAACTCTACCATG+AGG + Chr2:80504603-80504622 None:intergenic 40.0%
CAAGAATGATCGACCAATGT+TGG - Chr2:80504929-80504948 MsG0280011171.01.T01:intron 40.0%
GACAATGGCCATAAATCATC+AGG + Chr2:80504049-80504068 None:intergenic 40.0%
GAGAACCTTAAAAGGCTCAT+GGG - Chr2:80504535-80504554 MsG0280011171.01.T01:intron 40.0%
GTAACTCTTATTCCGACGTT+AGG + Chr2:80502913-80502932 None:intergenic 40.0%
GTTAGAGTTCATGTTCCTGT+CGG - Chr2:80502943-80502962 MsG0280011171.01.T01:CDS 40.0%
TCAACTTTCCAAGGAGGATA+TGG - Chr2:80504660-80504679 MsG0280011171.01.T01:intron 40.0%
TTAATCCACCTCCGGTATAA+GGG + Chr2:80505185-80505204 None:intergenic 40.0%
TTGTGGTTTAGAGAACGTAG+TGG - Chr2:80504459-80504478 MsG0280011171.01.T01:intron 40.0%
! AACTAAACAGGTCTTTTGGG+GGG - Chr2:80504100-80504119 MsG0280011171.01.T01:intron 40.0%
! ACTGGTTTCTTATTACCGAC+AGG + Chr2:80502961-80502980 None:intergenic 40.0%
!! ACTTTTGTATCGGCCAACAT+TGG + Chr2:80504945-80504964 None:intergenic 40.0%
!! GTATTCTTGTTTCTGAGGAG+TGG - Chr2:80504307-80504326 MsG0280011171.01.T01:intron 40.0%
!! TCTCCAATCTTTAAAGTGCG+GGG - Chr2:80503783-80503802 MsG0280011171.01.T01:intron 40.0%
!! TTGTCGACTTTCCCTTGTTA+GGG - Chr2:80504232-80504251 MsG0280011171.01.T01:intron 40.0%
AAACATCACACCCTCAAGAG+CGG + Chr2:80505107-80505126 None:intergenic 45.0%
AGAAAGAACTGGATCCGACA+AGG + Chr2:80504996-80505015 None:intergenic 45.0%
AGAACGTAGTGGTGCAAAGT+TGG - Chr2:80504470-80504489 MsG0280011171.01.T01:intron 45.0%
AGCGATTCAACTTTCCAAGG+AGG - Chr2:80504654-80504673 MsG0280011171.01.T01:intron 45.0%
AGTGATTAGATGCGTCTCTC+GGG + Chr2:80504365-80504384 None:intergenic 45.0%
ATGGGTAACCTCAAAAGGTG+GGG - Chr2:80504553-80504572 MsG0280011171.01.T01:intron 45.0%
CACAAAGCATGTCCCTAACA+AGG + Chr2:80504247-80504266 None:intergenic 45.0%
CAGTGATTAGATGCGTCTCT+CGG + Chr2:80504366-80504385 None:intergenic 45.0%
CATGGGTAACCTCAAAAGGT+GGG - Chr2:80504552-80504571 MsG0280011171.01.T01:intron 45.0%
CCACCACTACTAACCAAATG+AGG + Chr2:80503924-80503943 None:intergenic 45.0%
CCTCATTTGGTTAGTAGTGG+TGG - Chr2:80503921-80503940 MsG0280011171.01.T01:intron 45.0%
GCAAAGAATACCTAAGCACC+AGG + Chr2:80504390-80504409 None:intergenic 45.0%
GGAGAACCTTAAAAGGCTCA+TGG - Chr2:80504534-80504553 MsG0280011171.01.T01:intron 45.0%
TACTGGGGTTTCGTTTCAGT+GGG - Chr2:80503254-80503273 MsG0280011171.01.T01:intron 45.0%
TCATGGGTAACCTCAAAAGG+TGG - Chr2:80504551-80504570 MsG0280011171.01.T01:intron 45.0%
TGTCATGTGTTCTTCCCACA+TGG + Chr2:80503077-80503096 None:intergenic 45.0%
TGTTAGGGACATGCTTTGTG+TGG - Chr2:80504247-80504266 MsG0280011171.01.T01:intron 45.0%
TTGTATCACCCTTATACCGG+AGG - Chr2:80505174-80505193 MsG0280011171.01.T01:CDS 45.0%
! CTTTGGTAATCTCGACCTCA+TGG - Chr2:80504585-80504604 MsG0280011171.01.T01:intron 45.0%
! GAACCCCGCACTTTAAAGAT+TGG + Chr2:80503789-80503808 None:intergenic 45.0%
! GGTTACCCATGAGCCTTTTA+AGG + Chr2:80504543-80504562 None:intergenic 45.0%
! GTGGGTGCTTCTAAAGAGTA+AGG - Chr2:80504708-80504727 MsG0280011171.01.T01:intron 45.0%
!! ATGGGTTGAAAGTGTTTCGG+AGG - Chr2:80504861-80504880 MsG0280011171.01.T01:intron 45.0%
!! CTTGTCGACTTTCCCTTGTT+AGG - Chr2:80504231-80504250 MsG0280011171.01.T01:intron 45.0%
!! TCTTGTTTCTGAGGAGTGGT+GGG - Chr2:80504311-80504330 MsG0280011171.01.T01:intron 45.0%
!! TTCTTGTTTCTGAGGAGTGG+TGG - Chr2:80504310-80504329 MsG0280011171.01.T01:intron 45.0%
!!! TGTTTTTAATTTATATATTT+TGG - Chr2:80503159-80503178 MsG0280011171.01.T01:CDS 5.0%
AACTAGTGAGTGGGGTAGCT+GGG - Chr2:80504160-80504179 MsG0280011171.01.T01:intron 50.0%
ACTGGGGTTTCGTTTCAGTG+GGG - Chr2:80503255-80503274 MsG0280011171.01.T01:intron 50.0%
AGAAGTTATGGCCGCTCTTG+AGG - Chr2:80505093-80505112 MsG0280011171.01.T01:CDS 50.0%
AGGTGGGGTAAAGAGGTCTT+TGG - Chr2:80504568-80504587 MsG0280011171.01.T01:intron 50.0%
CCTCAAAAGGTGGGGTAAAG+AGG - Chr2:80504561-80504580 MsG0280011171.01.T01:intron 50.0%
CTACTGGGGTTTCGTTTCAG+TGG - Chr2:80503253-80503272 MsG0280011171.01.T01:intron 50.0%
CTCGTGCTCAACTAGTGAGT+GGG - Chr2:80504151-80504170 MsG0280011171.01.T01:intron 50.0%
GAAGTTATGGCCGCTCTTGA+GGG - Chr2:80505094-80505113 MsG0280011171.01.T01:CDS 50.0%
GGCTCATGGGTAACCTCAAA+AGG - Chr2:80504548-80504567 MsG0280011171.01.T01:intron 50.0%
TAATCACTGCCCTGGTGCTT+AGG - Chr2:80504377-80504396 MsG0280011171.01.T01:intron 50.0%
TATCACCCTTATACCGGAGG+TGG - Chr2:80505177-80505196 MsG0280011171.01.T01:CDS 50.0%
TCGTGCTCAACTAGTGAGTG+GGG - Chr2:80504152-80504171 MsG0280011171.01.T01:intron 50.0%
TCTCGTGCTCAACTAGTGAG+TGG - Chr2:80504150-80504169 MsG0280011171.01.T01:intron 50.0%
TGAATTCCCCCAACATGCAC+AGG + Chr2:80504129-80504148 None:intergenic 50.0%
TGCTTTGTGGGGGTTGTGTA+TGG - Chr2:80504005-80504024 MsG0280011171.01.T01:intron 50.0%
TTCCAAGGAGGATATGGCTC+TGG - Chr2:80504666-80504685 MsG0280011171.01.T01:intron 50.0%
TTCGAAGTTGACGCCGTTAC+TGG + Chr2:80502979-80502998 None:intergenic 50.0%
! CCTCTTTACCCCACCTTTTG+AGG + Chr2:80504564-80504583 None:intergenic 50.0%
ACTAGTGAGTGGGGTAGCTG+GGG - Chr2:80504161-80504180 MsG0280011171.01.T01:intron 55.0%
AGCCAGAGCCATATCCTCCT+TGG + Chr2:80504671-80504690 None:intergenic 55.0%
AGCTTGGGGTTGCTAGCCAA+TGG - Chr2:80504334-80504353 MsG0280011171.01.T01:intron 55.0%
CAACTAGTGAGTGGGGTAGC+TGG - Chr2:80504159-80504178 MsG0280011171.01.T01:intron 55.0%
CGTCTCTCGGGAATGTCCAT+TGG + Chr2:80504353-80504372 None:intergenic 55.0%
CTGGGGTTTCGTTTCAGTGG+GGG - Chr2:80503256-80503275 MsG0280011171.01.T01:intron 55.0%
GACGCATCTAATCACTGCCC+TGG - Chr2:80504369-80504388 MsG0280011171.01.T01:intron 55.0%
TGGGGGTTGTGTATGGAGTG+AGG - Chr2:80504012-80504031 MsG0280011171.01.T01:intron 55.0%
GGGATGCCTGTGCATGTTGG+GGG - Chr2:80504120-80504139 MsG0280011171.01.T01:intron 60.0%
GGGGATGCCTGTGCATGTTG+GGG - Chr2:80504119-80504138 MsG0280011171.01.T01:intron 60.0%
GGGGGATGCCTGTGCATGTT+GGG - Chr2:80504118-80504137 MsG0280011171.01.T01:intron 60.0%
! CTGAGGAGTGGTGGGAGCTT+GGG - Chr2:80504319-80504338 MsG0280011171.01.T01:intron 60.0%
! TCTGAGGAGTGGTGGGAGCT+TGG - Chr2:80504318-80504337 MsG0280011171.01.T01:intron 60.0%
! TGAGGAGTGGTGGGAGCTTG+GGG - Chr2:80504320-80504339 MsG0280011171.01.T01:intron 60.0%
GGGGGGATGCCTGTGCATGT+TGG - Chr2:80504117-80504136 MsG0280011171.01.T01:intron 65.0%
GGGTAGCTGGGGTCAGTAGG+CGG - Chr2:80504172-80504191 MsG0280011171.01.T01:intron 65.0%
GGTAGCTGGGGTCAGTAGGC+GGG - Chr2:80504173-80504192 MsG0280011171.01.T01:intron 65.0%
GTGGGGTAGCTGGGGTCAGT+AGG - Chr2:80504169-80504188 MsG0280011171.01.T01:intron 65.0%
AGCTGGGGTCAGTAGGCGGG+AGG - Chr2:80504176-80504195 MsG0280011171.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 80502832 80505204 80502832 ID=MsG0280011171.01;Name=MsG0280011171.01
Chr2 mRNA 80502832 80505204 80502832 ID=MsG0280011171.01.T01;Parent=MsG0280011171.01;Name=MsG0280011171.01.T01;_AED=0.60;_eAED=0.60;_QI=0|0|0|0.6|1|1|5|0|236
Chr2 exon 80505014 80505204 80505014 ID=MsG0280011171.01.T01:exon:21552;Parent=MsG0280011171.01.T01
Chr2 exon 80503854 80503926 80503854 ID=MsG0280011171.01.T01:exon:21551;Parent=MsG0280011171.01.T01
Chr2 exon 80503312 80503501 80503312 ID=MsG0280011171.01.T01:exon:21550;Parent=MsG0280011171.01.T01
Chr2 exon 80503123 80503182 80503123 ID=MsG0280011171.01.T01:exon:21549;Parent=MsG0280011171.01.T01
Chr2 exon 80502832 80503028 80502832 ID=MsG0280011171.01.T01:exon:21548;Parent=MsG0280011171.01.T01
Chr2 CDS 80505014 80505204 80505014 ID=MsG0280011171.01.T01:cds;Parent=MsG0280011171.01.T01
Chr2 CDS 80503854 80503926 80503854 ID=MsG0280011171.01.T01:cds;Parent=MsG0280011171.01.T01
Chr2 CDS 80503312 80503501 80503312 ID=MsG0280011171.01.T01:cds;Parent=MsG0280011171.01.T01
Chr2 CDS 80503123 80503182 80503123 ID=MsG0280011171.01.T01:cds;Parent=MsG0280011171.01.T01
Chr2 CDS 80502832 80503028 80502832 ID=MsG0280011171.01.T01:cds;Parent=MsG0280011171.01.T01
Gene Sequence

>MsG0280011171.01.T01

ATGAAGAGCAAAAACGGTTTAGCGTTGAAAGTGAAGTTGGATACAAAAGTGAAGGCTAAAGTGGGAAAAATGAAAACACCTAACGTCGGAATAAGAGTTACATGTGACGGTGTTAGAGTTCATGTTCCTGTCGGTAATAAGAAACCAGTAACGGCGTCAACTTCGAACGTTAAGTGTGATGTTGATGTTAGGTCTTTTGGGGGGATGCCTGTGCATGTTGGGGGAATTCAATCTCGTGCTCAACTAGTGAGTGGGGTAGCTGGGGAGAACCTTAAAAGGCTCATGGGTAACCTCAAAAGGTGGGGTAAAGAGGTCTTTGGTAATCTCGACCTCATGGTAGAGTTGTTAGTTGAGATGATTAAGGACTTGGATATAAAGATAGAAGCGATTCAACTTTCCAAGGAGGATATGGCTCTGGCTAAAGCCTTTGTTGAATTGTGGGTGCTTCTAAAGAGGAATATATGGGTTGAAAGTGTTTCGGAGGTCCGTGATGAAATACCTAGCTTCTTTAAAGTTCTTTCTGAAGAGATTCATGATGTAGTGTCTAATTTCTATGGTAACAAGAGTTCGGGTTCGAATGAGCTTAATCTCATTCTTCAAGAAGTTATGGCCGCTCTTGAGGGTGTGATGTTTGAGCAATTTTTTCTCCATGTCAATTTTCTACGAAGTTTTTCCTCTTATTGTATCACCCTTATACCGGAGGTGGATTAA
Protein sequence

>MsG0280011171.01.T01

MKSKNGLALKVKLDTKVKAKVGKMKTPNVGIRVTCDGVRVHVPVGNKKPVTASTSNVKCDVDVRSFGGMPVHVGGIQSRAQLVSGVAGENLKRLMGNLKRWGKEVFGNLDLMVELLVEMIKDLDIKIEAIQLSKEDMALAKAFVELWVLLKRNIWVESVSEVRDEIPSFFKVLSEEIHDVVSNFYGNKSSGSNELNLILQEVMAALEGVMFEQFFLHVNFLRSFSSYCITLIPEVD*