Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015455.01.T01 | XP_003600872.1 | 96.756 | 524 | 13 | 1 | 15 | 538 | 107 | 626 | 0 | 1041 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003232.01 | MsG0380015455.01 | 0.800994 | 1.109654e-48 | 5.240744e-46 |
| MsG0180003630.01 | MsG0380015455.01 | 0.818003 | 2.458272e-52 | 1.818211e-49 |
| MsG0180003638.01 | MsG0380015455.01 | 0.829827 | 4.139273e-55 | 4.285558e-52 |
| MsG0180003948.01 | MsG0380015455.01 | 0.831587 | 1.534294e-55 | 1.672474e-52 |
| MsG0180004005.01 | MsG0380015455.01 | 0.808444 | 3.087719e-50 | 1.765846e-47 |
| MsG0180004300.01 | MsG0380015455.01 | 0.819167 | 1.338691e-52 | 1.022396e-49 |
| MsG0180004382.01 | MsG0380015455.01 | 0.822437 | 2.370421e-53 | 1.984046e-50 |
| MsG0180004651.01 | MsG0380015455.01 | 0.800385 | 1.477555e-48 | 6.870734e-46 |
| MsG0180005296.01 | MsG0380015455.01 | 0.858276 | 9.207423e-63 | 2.337128e-59 |
| MsG0180005614.01 | MsG0380015455.01 | 0.808581 | 2.886253e-50 | 1.656427e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015455.01.T01 | MTR_3g070310 | 96.756 | 524 | 13 | 1 | 15 | 538 | 107 | 626 | 0.0 | 1041 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015455.01.T01 | AT5G05700 | 49.270 | 548 | 231 | 10 | 15 | 537 | 31 | 556 | 1.51e-162 | 474 |
| MsG0380015455.01.T01 | AT5G05700 | 49.270 | 548 | 231 | 10 | 15 | 537 | 106 | 631 | 2.51e-161 | 473 |
| MsG0380015455.01.T01 | AT3G11240 | 48.030 | 533 | 229 | 9 | 15 | 537 | 67 | 561 | 1.15e-153 | 451 |
| MsG0380015455.01.T01 | AT3G11240 | 47.744 | 532 | 232 | 9 | 15 | 537 | 110 | 604 | 2.89e-153 | 452 |
| MsG0380015455.01.T01 | AT3G11240 | 50.529 | 473 | 194 | 7 | 74 | 537 | 4 | 445 | 2.20e-150 | 439 |
| MsG0380015455.01.T01 | AT3G11240 | 50.529 | 473 | 194 | 7 | 74 | 537 | 4 | 445 | 2.20e-150 | 439 |
| MsG0380015455.01.T01 | AT3G11240 | 50.529 | 473 | 194 | 7 | 74 | 537 | 4 | 445 | 2.20e-150 | 439 |
Find 124 sgRNAs with CRISPR-Local
Find 199 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGGTATTAGCCAAT+GGG | 0.524221 | 3:+70433288 | MsG0380015455.01.T01:CDS |
| AAAGAACCATCGGTGAAAAG+TGG | 0.547954 | 3:-70432982 | None:intergenic |
| AAAGAGCCAAAACCACAAGG+AGG | 0.739577 | 3:-70433812 | None:intergenic |
| AAGATCCTGTATCCAGCAGC+TGG | 0.502219 | 3:+70435404 | MsG0380015455.01.T01:three_prime_UTR |
| AAGGATCTGCAAAGTGTATT+TGG | 0.341290 | 3:+70435229 | MsG0380015455.01.T01:intron |
| ACACAGAAAGCTCATATCGC+AGG | 0.558720 | 3:+70433735 | MsG0380015455.01.T01:CDS |
| ACAGTTCCTCCTTGTGGTTT+TGG | 0.260938 | 3:+70433806 | MsG0380015455.01.T01:CDS |
| ACAGTTGCAGAAATACAGGA+GGG | 0.725694 | 3:+70435279 | MsG0380015455.01.T01:CDS |
| ACATTTCTGGTCTGTCTATC+AGG | 0.275790 | 3:+70433492 | MsG0380015455.01.T01:CDS |
| ACCAAAAGCTTCAGTCAGAA+TGG | 0.453937 | 3:+70433253 | MsG0380015455.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAATGGTTGAATCTGAAT+TGG | + | Chr3:70434628-70434647 | MsG0380015455.01.T01:CDS | 30.0% | |
| AGCCTTCTTTCACATAATAT+AGG | - | Chr3:70434370-70434389 | None:intergenic | 30.0% | |
| ATAGCGAGTATGTTTCAAAA+TGG | + | Chr3:70434875-70434894 | MsG0380015455.01.T01:intron | 30.0% | |
| ATATTCTTCTGGATCAAAAC+TGG | - | Chr3:70433663-70433682 | None:intergenic | 30.0% | |
| ATGATTTATTTGTCCCATTC+TGG | + | Chr3:70435156-70435175 | MsG0380015455.01.T01:intron | 30.0% | |
| CACCTATATTATGTGAAAGA+AGG | + | Chr3:70434365-70434384 | MsG0380015455.01.T01:intron | 30.0% | |
| CTTCTTTCACATAATATAGG+TGG | - | Chr3:70434367-70434386 | None:intergenic | 30.0% | |
| GAAAAGTGGAACAAACAAAT+TGG | - | Chr3:70432971-70432990 | None:intergenic | 30.0% | |
| TAAAACTATTGCAGAAAAGC+TGG | + | Chr3:70433445-70433464 | MsG0380015455.01.T01:CDS | 30.0% | |
| TATAGTAGAAGACTATGAAG+AGG | - | Chr3:70434298-70434317 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 70432963 | 70435644 | 70432963 | ID=MsG0380015455.01;Name=MsG0380015455.01 |
| Chr3 | mRNA | 70432963 | 70435644 | 70432963 | ID=MsG0380015455.01.T01;Parent=MsG0380015455.01;Name=MsG0380015455.01.T01;_AED=0.44;_eAED=0.46;_QI=0|1|0.66|1|1|1|3|296|538 |
| Chr3 | exon | 70432963 | 70434116 | 70432963 | ID=MsG0380015455.01.T01:exon:23620;Parent=MsG0380015455.01.T01 |
| Chr3 | exon | 70434415 | 70434760 | 70434415 | ID=MsG0380015455.01.T01:exon:23621;Parent=MsG0380015455.01.T01 |
| Chr3 | exon | 70435232 | 70435644 | 70435232 | ID=MsG0380015455.01.T01:exon:23622;Parent=MsG0380015455.01.T01 |
| Chr3 | CDS | 70432963 | 70434116 | 70432963 | ID=MsG0380015455.01.T01:cds;Parent=MsG0380015455.01.T01 |
| Chr3 | CDS | 70434415 | 70434760 | 70434415 | ID=MsG0380015455.01.T01:cds;Parent=MsG0380015455.01.T01 |
| Chr3 | CDS | 70435232 | 70435348 | 70435232 | ID=MsG0380015455.01.T01:cds;Parent=MsG0380015455.01.T01 |
| Chr3 | three_prime_UTR | 70435349 | 70435644 | 70435349 | ID=MsG0380015455.01.T01:three_prime_utr;Parent=MsG0380015455.01.T01 |
| Gene Sequence |
>MsG0380015455.01.T01 ATGTTCCAATTTGTTTGTTCCACTTTTCACCGATGGTTCTTTCTTCCTAGGTTTTTAGATGGAACCTTGGATGTTAAAAGAGTTGATGCCATGGAGGAACCAAATACTTCAAACAATTCAGAAAGCCTTGTCCATAACAACCCATCATGCTCCATGTCAGAAGAATCTCTATCTGTTAGCAATAAAGAGGTGGATGAAGTTGAAAAATCTTTGCACTATTTATCCGATCAGATTGATAATGCAGTGCACATGTTCACTGAGAACGGGGAATTTCCATCTGGTATTCAATTACCAAAAGCTTCAGTCAGAATGGTTTCACAGGGGAAAAAGAAGGTATTAGCCAATGGGTCCGAAGACCTCTTATATAGTAGCAATATAGCCTTTCAAGTTGCTGCATCTATAAACCGAGCAAAGTCAAGGGACAAGGATGGTAATGATTCTAAACCATCAAGAGATAGTGAGAAGGAGAATGGATTTTCTCCTAAAACTATTGCAGAAAAGCTGGTAGCATCTTTAGATTCAACAGTGAACATTTCTGGTCTGTCTATCAGGGCTTGCAATGGACATGTAAATTTTTATGCTTCTAGTAAGCAAGTTTCCCAAAATGTTACTATTCAAAAAGCTGCAATTCCAAAGAAGTCTGCAACGAATCGTAATATTGGAGGAAAGCGAAAACTTGAGATTCGTTTGAACAGATCCAGTTTTGATCCAGAAGAATATGCTTTATATAGAAAATATCAGAGAAAAGTACATAATGATAAACCAGAAAATTACACAGAAAGCTCATATCGCAGGTTTTTGGTTGATACTCCATTAATATATGTTTCACATACTGGTGATAACACAGTTCCTCCTTGTGGTTTTGGCTCTTTCCATCAACAGTATTTAGTGGATGGTAAATTAGTGGCAGTTGGGGTTATAGATATTCTTCCTAACTGTTTATCGAGTAAATATTTGTTCTGGGATCCAGACTTTGCCTTTCTATCACTAGGCAAGTACTCTGCTCTTCAAGAAATTGGTTGGGTGAAAGATAACCAGGCCTATTGTCCTAGTCTCCAGTACTATTATCTTGGCTACTATATTCACTCTTGCAACAAGATGAGATACAAAGCTGCATATCGCCCTTCAGAGCTACTATGTCCTCTTCGCTATCAGTGGGTTTCATTTGACATTGCAAGGCCTTTGCTTGACAGAAAGCGTTATGTTGTCTTATCAGATGCTTCCATTTTACAAAATGGAGAGTCATCCCCACTTCAAGTTGCTGAAGATCAAGACACTATGTTAAGCCAGGTTGATGACAGTGGTCAAGAAGATGCAAATGATGTTCCAATGCATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGAAATGGTTGAATCTGAATTGGAAAGTTCTGATGATGAACCTGATATAGAAACTACTTCAAATGGTGAACCAGAAAATGGCGATTTCAGCAAAGTTTTGCTAGGGATAAAGGGATCCCGTGTGAGATACAAGGATCTGCAAAGTGTATTTGGTCGTGAGCAGCAGAGATACTTGGAGTCACAGTTGCAGAAATACAGGAGGGTTGTCGGTCCAGTGTTATCCGAGCGAATGGTCTACTCTCTCGGTTAA |
| Protein sequence |
>MsG0380015455.01.T01 MFQFVCSTFHRWFFLPRFLDGTLDVKRVDAMEEPNTSNNSESLVHNNPSCSMSEESLSVSNKEVDEVEKSLHYLSDQIDNAVHMFTENGEFPSGIQLPKASVRMVSQGKKKVLANGSEDLLYSSNIAFQVAASINRAKSRDKDGNDSKPSRDSEKENGFSPKTIAEKLVASLDSTVNISGLSIRACNGHVNFYASSKQVSQNVTIQKAAIPKKSATNRNIGGKRKLEIRLNRSSFDPEEYALYRKYQRKVHNDKPENYTESSYRRFLVDTPLIYVSHTGDNTVPPCGFGSFHQQYLVDGKLVAVGVIDILPNCLSSKYLFWDPDFAFLSLGKYSALQEIGWVKDNQAYCPSLQYYYLGYYIHSCNKMRYKAAYRPSELLCPLRYQWVSFDIARPLLDRKRYVVLSDASILQNGESSPLQVAEDQDTMLSQVDDSGQEDANDVPMHEDEDEDEDEDEEMVESELESSDDEPDIETTSNGEPENGDFSKVLLGIKGSRVRYKDLQSVFGREQQRYLESQLQKYRRVVGPVLSERMVYSLG* |
