Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003688.01.T01 | XP_003590511.1 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 8.93E-90 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003688.01.T01 | P93354 | 80.822 | 146 | 18 | 2 | 1 | 136 | 1 | 146 | 1.16E-76 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003688.01.T01 | G7IBM3 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 4.26e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000135.01 | MsG0180003688.01 | 0.815115 | 1.090352e-51 | 7.450830e-49 |
| MsG0180003688.01 | MsG0380017800.01 | 0.810270 | 1.252772e-50 | 7.518150e-48 |
| MsG0180003688.01 | MsG0480021265.01 | 0.836607 | 8.475536e-57 | 1.073424e-53 |
| MsG0180003688.01 | MsG0580025408.01 | 0.817054 | 4.023664e-52 | 2.899659e-49 |
| MsG0180003688.01 | MsG0780040845.01 | 0.817551 | 3.109373e-52 | 2.271414e-49 |
| MsG0180003688.01 | MsG0880044990.01 | 0.808299 | 3.315344e-50 | 1.888756e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880042844.01 | MsG0180003688.01 | PPI |
| MsG0480020877.01 | MsG0180003688.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003688.01.T01 | MTR_1g068600 | 99.265 | 136 | 1 | 0 | 1 | 136 | 1 | 136 | 1.08e-93 | 266 |
| MsG0180003688.01.T01 | MTR_4g063240 | 85.034 | 147 | 11 | 2 | 1 | 136 | 1 | 147 | 1.84e-81 | 236 |
| MsG0180003688.01.T01 | MTR_4g063220 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003688.01.T01 | MTR_4g063990 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003688.01.T01 | MTR_4g063200 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003688.01.T01 | MTR_4g064020 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.58e-78 | 228 |
| MsG0180003688.01.T01 | MTR_1g090780 | 91.304 | 138 | 9 | 2 | 1 | 136 | 1 | 137 | 5.32e-78 | 227 |
| MsG0180003688.01.T01 | MTR_1g492910 | 91.304 | 138 | 9 | 2 | 1 | 136 | 1 | 137 | 6.14e-78 | 227 |
| MsG0180003688.01.T01 | MTR_4g071180 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.48e-77 | 225 |
| MsG0180003688.01.T01 | MTR_4g070240 | 85.811 | 148 | 9 | 2 | 1 | 136 | 1 | 148 | 4.48e-77 | 225 |
| MsG0180003688.01.T01 | MTR_4g088025 | 86.713 | 143 | 12 | 2 | 1 | 136 | 1 | 143 | 9.64e-76 | 221 |
| MsG0180003688.01.T01 | MTR_4g070245 | 84.459 | 148 | 11 | 2 | 1 | 136 | 1 | 148 | 1.60e-75 | 221 |
| MsG0180003688.01.T01 | MTR_4g063275 | 83.108 | 148 | 13 | 2 | 1 | 136 | 1 | 148 | 5.06e-75 | 220 |
| MsG0180003688.01.T01 | MTR_4g064983 | 84.722 | 144 | 10 | 2 | 1 | 132 | 1 | 144 | 2.63e-73 | 219 |
| MsG0180003688.01.T01 | MTR_7g099960 | 95.370 | 108 | 4 | 1 | 30 | 136 | 31 | 138 | 3.00e-64 | 192 |
| MsG0180003688.01.T01 | MTR_2g084480 | 62.921 | 89 | 33 | 0 | 47 | 135 | 136 | 224 | 1.02e-37 | 128 |
| MsG0180003688.01.T01 | MTR_8g069990 | 71.591 | 88 | 23 | 2 | 8 | 95 | 3 | 88 | 6.24e-35 | 117 |
| MsG0180003688.01.T01 | MTR_4g063270 | 90.196 | 51 | 5 | 0 | 74 | 124 | 1 | 51 | 5.97e-27 | 95.5 |
| MsG0180003688.01.T01 | MTR_7g114580 | 90.244 | 41 | 4 | 0 | 96 | 136 | 11 | 51 | 7.28e-20 | 77.4 |
| MsG0180003688.01.T01 | MTR_4g032777 | 39.744 | 78 | 47 | 0 | 43 | 120 | 33 | 110 | 1.47e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003688.01.T01 | AT3G45980 | 83.333 | 150 | 11 | 2 | 1 | 136 | 1 | 150 | 6.24e-77 | 225 |
| MsG0180003688.01.T01 | AT2G37470 | 88.406 | 138 | 13 | 2 | 1 | 135 | 1 | 138 | 8.11e-76 | 221 |
| MsG0180003688.01.T01 | AT1G07790 | 83.784 | 148 | 12 | 2 | 1 | 136 | 1 | 148 | 2.43e-75 | 221 |
| MsG0180003688.01.T01 | AT2G28720 | 83.444 | 151 | 10 | 2 | 1 | 136 | 1 | 151 | 2.35e-74 | 218 |
| MsG0180003688.01.T01 | AT3G53650 | 87.681 | 138 | 15 | 2 | 1 | 136 | 1 | 138 | 1.62e-73 | 216 |
| MsG0180003688.01.T01 | AT5G59910 | 87.407 | 135 | 14 | 1 | 5 | 136 | 16 | 150 | 6.45e-73 | 214 |
| MsG0180003688.01.T01 | AT3G46030 | 88.372 | 129 | 12 | 1 | 11 | 136 | 17 | 145 | 1.93e-71 | 211 |
| MsG0180003688.01.T01 | AT5G02570 | 83.571 | 140 | 11 | 2 | 1 | 136 | 1 | 132 | 1.47e-68 | 203 |
| MsG0180003688.01.T01 | AT3G09480 | 80.147 | 136 | 17 | 1 | 1 | 136 | 1 | 126 | 1.28e-65 | 195 |
| MsG0180003688.01.T01 | AT5G22880 | 81.119 | 143 | 17 | 2 | 4 | 136 | 3 | 145 | 2.72e-65 | 195 |
| MsG0180003688.01.T01 | AT1G08170 | 62.353 | 85 | 32 | 0 | 49 | 133 | 151 | 235 | 9.20e-38 | 128 |
Find 35 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGAAGATGTAAATCTTAT+AGG | 0.133504 | 1:+66630003 | None:intergenic |
| TAATTAAGAGCTAGTAAATT+TGG | 0.233127 | 1:+66629754 | None:intergenic |
| TTCTTTCGAGATCTTCTTCT+CGG | 0.293193 | 1:+66630069 | None:intergenic |
| AGCGGGTTTCTTCTCTGCCT+TGG | 0.302939 | 1:+66630138 | None:intergenic |
| GCTGTGCGTTTGGTTCTTCC+TGG | 0.321985 | 1:-66629821 | MsG0180003688.01.T01:CDS |
| TTTGAAGAAATACCTATATC+AGG | 0.334896 | 1:+66629962 | None:intergenic |
| GCGGGTTTCTTCTCTGCCTT+GGG | 0.359903 | 1:+66630139 | None:intergenic |
| CATCTTCAAGGTTCTCAAAC+AGG | 0.361477 | 1:-66629990 | MsG0180003688.01.T01:CDS |
| GAGATCTTCTTCTCGGCTCT+TGG | 0.362586 | 1:+66630076 | None:intergenic |
| CTTCTCTGCCTTGGGAGCCA+TGG | 0.363767 | 1:+66630147 | None:intergenic |
| CTATAAGATTTACATCTTCA+AGG | 0.370441 | 1:-66630002 | MsG0180003688.01.T01:CDS |
| GATTCAAACCGCTGTGCGTT+TGG | 0.390239 | 1:-66629831 | MsG0180003688.01.T01:CDS |
| TTTGGTTCTTCCTGGTGAAT+TGG | 0.398260 | 1:-66629813 | MsG0180003688.01.T01:CDS |
| AAACAGGTTCATCCTGATAT+AGG | 0.421612 | 1:-66629974 | MsG0180003688.01.T01:CDS |
| AGGTATTTCTTCAAAAGCTA+TGG | 0.429032 | 1:-66629954 | MsG0180003688.01.T01:CDS |
| AGTAAATTTGGTAACAGCCT+TGG | 0.444512 | 1:+66629766 | None:intergenic |
| CTTGTTGTAACGAGCCAATC+TGG | 0.445538 | 1:+66629877 | None:intergenic |
| TCCGCGGGTTTCTTCTCAGC+GGG | 0.464857 | 1:+66630121 | None:intergenic |
| TCTGCTGGTGATTTCTCCGC+GGG | 0.470002 | 1:+66630106 | None:intergenic |
| CGCTCAGGAAGCTTCCAGAT+TGG | 0.475450 | 1:-66629891 | MsG0180003688.01.T01:CDS |
| GCATGTTTCGCCAATTCACC+AGG | 0.516345 | 1:+66629803 | None:intergenic |
| GGTATTTCTTCAAAAGCTAT+GGG | 0.517184 | 1:-66629953 | MsG0180003688.01.T01:CDS |
| GCGAAACATGCTGTTTCTGA+AGG | 0.530754 | 1:-66629791 | MsG0180003688.01.T01:CDS |
| ATTCAGATCCATGGCTCCCA+AGG | 0.541897 | 1:-66630155 | None:intergenic |
| GAAACCGACGATTACGTCAC+GGG | 0.556515 | 1:-66629855 | MsG0180003688.01.T01:CDS |
| CTCTGCTGGTGATTTCTCCG+CGG | 0.560516 | 1:+66630105 | None:intergenic |
| GAGAAGAAGATCTCGAAAGA+AGG | 0.564931 | 1:-66630067 | MsG0180003688.01.T01:CDS |
| TATCTTTGAGAAACTCGCTC+AGG | 0.586744 | 1:-66629906 | MsG0180003688.01.T01:CDS |
| ATCTCCCGTGACGTAATCGT+CGG | 0.590908 | 1:+66629851 | None:intergenic |
| TGCTGTTTCTGAAGGAACCA+AGG | 0.603820 | 1:-66629783 | MsG0180003688.01.T01:CDS |
| CTCCGCGGGTTTCTTCTCAG+CGG | 0.628471 | 1:+66630120 | None:intergenic |
| AAGAAGATCTCGAAAGAAGG+AGG | 0.628797 | 1:-66630064 | MsG0180003688.01.T01:CDS |
| AGAAACCGACGATTACGTCA+CGG | 0.672005 | 1:-66629856 | MsG0180003688.01.T01:CDS |
| ACCCGCTGAGAAGAAACCCG+CGG | 0.680467 | 1:-66630122 | MsG0180003688.01.T01:CDS |
| AGGAAGAACCAAACGCACAG+CGG | 0.757762 | 1:+66629823 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTATAAGATTTACATCTTCA+AGG | - | Chr1:66629900-66629919 | MsG0180003688.01.T01:CDS | 25.0% |
| ! | CTTGAAGATGTAAATCTTAT+AGG | + | Chr1:66629902-66629921 | None:intergenic | 25.0% |
| ! | TTTGAAGAAATACCTATATC+AGG | + | Chr1:66629943-66629962 | None:intergenic | 25.0% |
| AGGTATTTCTTCAAAAGCTA+TGG | - | Chr1:66629948-66629967 | MsG0180003688.01.T01:CDS | 30.0% | |
| GGTATTTCTTCAAAAGCTAT+GGG | - | Chr1:66629949-66629968 | MsG0180003688.01.T01:CDS | 30.0% | |
| AAACAGGTTCATCCTGATAT+AGG | - | Chr1:66629928-66629947 | MsG0180003688.01.T01:CDS | 35.0% | |
| TTCTTTCGAGATCTTCTTCT+CGG | + | Chr1:66629836-66629855 | None:intergenic | 35.0% | |
| ! | AGTAAATTTGGTAACAGCCT+TGG | + | Chr1:66630139-66630158 | None:intergenic | 35.0% |
| AAGAAGATCTCGAAAGAAGG+AGG | - | Chr1:66629838-66629857 | MsG0180003688.01.T01:CDS | 40.0% | |
| CATCTTCAAGGTTCTCAAAC+AGG | - | Chr1:66629912-66629931 | MsG0180003688.01.T01:CDS | 40.0% | |
| GAGAAGAAGATCTCGAAAGA+AGG | - | Chr1:66629835-66629854 | MsG0180003688.01.T01:CDS | 40.0% | |
| ! | TATCTTTGAGAAACTCGCTC+AGG | - | Chr1:66629996-66630015 | MsG0180003688.01.T01:CDS | 40.0% |
| !! | TTTGGTTCTTCCTGGTGAAT+TGG | - | Chr1:66630089-66630108 | MsG0180003688.01.T01:CDS | 40.0% |
| AGAAACCGACGATTACGTCA+CGG | - | Chr1:66630046-66630065 | MsG0180003688.01.T01:CDS | 45.0% | |
| ! | CTTGTTGTAACGAGCCAATC+TGG | + | Chr1:66630028-66630047 | None:intergenic | 45.0% |
| !! | GCGAAACATGCTGTTTCTGA+AGG | - | Chr1:66630111-66630130 | MsG0180003688.01.T01:CDS | 45.0% |
| !! | TGCTGTTTCTGAAGGAACCA+AGG | - | Chr1:66630119-66630138 | MsG0180003688.01.T01:CDS | 45.0% |
| AGGAAGAACCAAACGCACAG+CGG | + | Chr1:66630082-66630101 | None:intergenic | 50.0% | |
| ATCTCCCGTGACGTAATCGT+CGG | + | Chr1:66630054-66630073 | None:intergenic | 50.0% | |
| GAAACCGACGATTACGTCAC+GGG | - | Chr1:66630047-66630066 | MsG0180003688.01.T01:CDS | 50.0% | |
| GAGATCTTCTTCTCGGCTCT+TGG | + | Chr1:66629829-66629848 | None:intergenic | 50.0% | |
| GATTCAAACCGCTGTGCGTT+TGG | - | Chr1:66630071-66630090 | MsG0180003688.01.T01:CDS | 50.0% | |
| GCATGTTTCGCCAATTCACC+AGG | + | Chr1:66630102-66630121 | None:intergenic | 50.0% | |
| !! | GCTCTTGGCTTTTTCTCTGC+TGG | + | Chr1:66629814-66629833 | None:intergenic | 50.0% |
| CGCTCAGGAAGCTTCCAGAT+TGG | - | Chr1:66630011-66630030 | MsG0180003688.01.T01:CDS | 55.0% | |
| CTCTGCTGGTGATTTCTCCG+CGG | + | Chr1:66629800-66629819 | None:intergenic | 55.0% | |
| GCGGGTTTCTTCTCTGCCTT+GGG | + | Chr1:66629766-66629785 | None:intergenic | 55.0% | |
| TCTGCTGGTGATTTCTCCGC+GGG | + | Chr1:66629799-66629818 | None:intergenic | 55.0% | |
| ! | AGCGGGTTTCTTCTCTGCCT+TGG | + | Chr1:66629767-66629786 | None:intergenic | 55.0% |
| !! | GCTGTGCGTTTGGTTCTTCC+TGG | - | Chr1:66630081-66630100 | MsG0180003688.01.T01:CDS | 55.0% |
| ACCCGCTGAGAAGAAACCCG+CGG | - | Chr1:66629780-66629799 | MsG0180003688.01.T01:CDS | 60.0% | |
| CTCCGCGGGTTTCTTCTCAG+CGG | + | Chr1:66629785-66629804 | None:intergenic | 60.0% | |
| TCCGCGGGTTTCTTCTCAGC+GGG | + | Chr1:66629784-66629803 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 66629757 | 66630167 | 66629757 | ID=MsG0180003688.01;Name=MsG0180003688.01 |
| Chr1 | mRNA | 66629757 | 66630167 | 66629757 | ID=MsG0180003688.01.T01;Parent=MsG0180003688.01;Name=MsG0180003688.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136 |
| Chr1 | exon | 66629757 | 66630167 | 66629757 | ID=MsG0180003688.01.T01:exon:3586;Parent=MsG0180003688.01.T01 |
| Chr1 | CDS | 66629757 | 66630167 | 66629757 | ID=MsG0180003688.01.T01:cds;Parent=MsG0180003688.01.T01 |
| Gene Sequence |
| Protein sequence |
