AlfalfaGEDB | MsG0180003991.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003991.01

KEGG: -
GO: GO:0000387
PFAM: PF01423 PF14541 PF14543

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003991.01.T01	KAG8370716.1	54.762	378	96	9	1	318	175	537	5.25E-115	354

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003991.01.T01	P0DO21	49.802	253	63	5	1	195	180	426	7.38E-67	219

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003991.01.T01	A0A5A7RD17	52.442	389	104	9	1	318	175	553	2.96e-114	352

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003991.01	MsG0380016201.01	0.852413	4.692475e-61	9.796890e-58
MsG0180003991.01	MsG0480019188.01	-0.819845	9.378181e-53	7.299209e-50
MsG0180003991.01	MsG0680030994.01	-0.800120	1.672645e-48	7.725658e-46
MsG0180003991.01	MsG0780040559.01	0.847243	1.308123e-59	2.310710e-56
MsG0180003991.01	MsG0780041191.01	-0.804711	1.894573e-49	9.837729e-47
MsG0180003991.01	MsG0780041301.01	-0.805173	1.516647e-49	7.969572e-47
MsG0180003991.01	MsG0880047306.01	-0.810969	8.846978e-51	5.409511e-48

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003991.01.T01	MTR_1g072420	63.200	250	31	4	1	195	179	422	9.59e-92	281
MsG0180003991.01.T01	MTR_1g072380	64.940	251	32	5	1	195	188	438	5.68e-88	271
MsG0180003991.01.T01	MTR_1g072450	60.000	250	38	4	1	195	179	421	7.60e-85	263
MsG0180003991.01.T01	MTR_1g072370	100.000	91	0	0	228	318	5	95	2.52e-59	187
MsG0180003991.01.T01	MTR_7g094680	100.000	91	0	0	228	318	5	95	1.14e-58	185
MsG0180003991.01.T01	MTR_1g093100	44.574	258	64	6	1	188	169	417	5.16e-56	188
MsG0180003991.01.T01	MTR_2g022450	43.083	253	79	7	1	195	172	417	4.92e-49	170
MsG0180003991.01.T01	MTR_2g022320	38.735	253	83	5	3	193	167	409	3.06e-41	149
MsG0180003991.01.T01	MTR_4g117090	37.209	258	88	6	3	193	192	442	2.60e-40	147
MsG0180003991.01.T01	MTR_2g022350	38.492	252	84	5	3	195	176	415	1.60e-38	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003991.01.T01	AT1G03220	46.640	253	69	6	1	195	176	420	9.89e-60	198
MsG0180003991.01.T01	AT4G02840	92.473	93	7	0	228	320	5	97	2.16e-56	179
MsG0180003991.01.T01	AT3G07590	91.209	91	8	0	228	318	5	95	3.75e-55	176
MsG0180003991.01.T01	AT3G07590	91.209	91	8	0	228	318	5	95	3.75e-55	176
MsG0180003991.01.T01	AT4G02840	83.495	103	7	1	228	320	5	107	5.76e-53	171
MsG0180003991.01.T01	AT1G03230	41.502	253	82	6	1	195	177	421	1.22e-48	169
MsG0180003991.01.T01	AT3G07590	89.231	65	7	0	254	318	5	69	2.37e-36	127
MsG0180003991.01.T01	AT5G19100	33.482	224	89	7	3	183	161	367	9.61e-25	103
MsG0180003991.01.T01	AT5G48430	29.150	247	101	7	1	192	165	392	5.70e-21	93.6
MsG0180003991.01.T01	AT5G19110	25.214	234	105	5	14	193	175	392	8.78e-14	72.4

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Find 70 sgRNAs with CRISPR-Local

Find 458 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGAAGCTAGTTGAGA+TGG	0.497173	1:+71469142	None:intergenic
AAAGCAGAAGCTGTGCTAAC+AGG	0.406748	1:+71468965	None:intergenic
AACAGTTAAACTAACACTGA+AGG	0.539350	1:-71462740	MsG0180003991.01.T01:CDS
AAGAACGGTACAATTGTTCA+TGG	0.494710	1:-71465820	MsG0180003991.01.T01:CDS
AAGCCTAAGAAGCCAACTGC+TGG	0.548844	1:-71462601	MsG0180003991.01.T01:CDS
AATCAAAATCGATGGAAAAG+TGG	0.521939	1:-71468897	MsG0180003991.01.T01:CDS
AATGGTGAATATAAATGATG+AGG	0.569327	1:-71468634	MsG0180003991.01.T01:CDS
AATGTGATTTGGAGCATGTT+TGG	0.433593	1:-71468666	MsG0180003991.01.T01:CDS
AATTTGCCAGGGACGCCATT+GGG	0.481307	1:-71468726	MsG0180003991.01.T01:intron
ACAGCTTCTGCTTTCTTGCA+GGG	0.511733	1:-71468956	MsG0180003991.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACGGTCTTAGAAAAAAG+AGG	+	Chr1:71464755-71464774	None:intergenic	30.0%
AAAAGAATTGCTTCATCTAG+AGG	-	Chr1:71464630-71464649	MsG0180003991.01.T01:intron	30.0%
AAACCAACAAAAGCCAAATT+TGG	+	Chr1:71465373-71465392	None:intergenic	30.0%
AAATTCAAGAAGGGTTTAAC+GGG	+	Chr1:71466073-71466092	None:intergenic	30.0%
AACACTCATGACAAAAAGAT+TGG	+	Chr1:71465342-71465361	None:intergenic	30.0%
AACAGTTAAACTAACACTGA+AGG	-	Chr1:71468960-71468979	MsG0180003991.01.T01:CDS	30.0%
AATCAAAATCGATGGAAAAG+TGG	-	Chr1:71462803-71462822	MsG0180003991.01.T01:intron	30.0%
AATGGCTTAGATTAGGTAAA+AGG	+	Chr1:71468397-71468416	None:intergenic	30.0%
AATTACATGGTCTAAGAAAG+TGG	-	Chr1:71467927-71467946	MsG0180003991.01.T01:intron	30.0%
ACACTCATGACAAAAAGATT+GGG	+	Chr1:71465341-71465360	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	71462525	71469197	71462525	ID=MsG0180003991.01;Name=MsG0180003991.01
Chr1	mRNA	71462525	71469197	71462525	ID=MsG0180003991.01.T01;Parent=MsG0180003991.01;Name=MsG0180003991.01.T01;_AED=0.49;_eAED=0.57;_QI=0\|0.25\|0\|1\|0.5\|0.4\|5\|0\|342
Chr1	exon	71468858	71469197	71468858	ID=MsG0180003991.01.T01:exon:4854;Parent=MsG0180003991.01.T01
Chr1	exon	71468496	71468738	71468496	ID=MsG0180003991.01.T01:exon:4853;Parent=MsG0180003991.01.T01
Chr1	exon	71468220	71468296	71468220	ID=MsG0180003991.01.T01:exon:4852;Parent=MsG0180003991.01.T01
Chr1	exon	71465809	71465908	71465809	ID=MsG0180003991.01.T01:exon:4851;Parent=MsG0180003991.01.T01
Chr1	exon	71462525	71462793	71462525	ID=MsG0180003991.01.T01:exon:4850;Parent=MsG0180003991.01.T01
Chr1	CDS	71468858	71469197	71468858	ID=MsG0180003991.01.T01:cds;Parent=MsG0180003991.01.T01
Chr1	CDS	71468496	71468738	71468496	ID=MsG0180003991.01.T01:cds;Parent=MsG0180003991.01.T01
Chr1	CDS	71468220	71468296	71468220	ID=MsG0180003991.01.T01:cds;Parent=MsG0180003991.01.T01
Chr1	CDS	71465809	71465908	71465809	ID=MsG0180003991.01.T01:cds;Parent=MsG0180003991.01.T01
Chr1	CDS	71462525	71462793	71462525	ID=MsG0180003991.01.T01:cds;Parent=MsG0180003991.01.T01

Gene Sequence

>MsG0180003991.01.T01

ATGGCCGGTCTTGGAAGAACCAAAATCGCTCTTCCATCTCAACTAGCTTCTGCTTTTAGCTTCGAAAGAAAATTTGCTTTTTGTTTTTCTTCTTCAGATGGTGTCATCATCTTCGGTGATGGTCCTTACAGTTTTCTCGCTGACAACGCTTCTCTCCCTAACGTTGTATTTGATTCAAAATCATTCACATACACGCCGCTTTTAATCAATCCTGTTAGCACAGCTTCTGCTTTCTTGCAGGGAGAGCCTTCAGTTGAGTATTTCATCGGAGTGAAAACAATCAAAATCGATGGAAAAGTGGTTTCACTTAACTCGAGTTTGTTGTCTATCGACAACAAAGGGACGCCATTGGGTGCGGCTGTGCCTACAATTGAGCTTGTTTTACAAAACAATGTGATTTGGAGCATGTTTGGTGCAAATTCAATGGTGAATATAAATGATGAGGTTTTGTGCCTTGGATTTGTTAACGGTGGTGAGAATGCAAGGACTTCTATTGTGATTGGAGGGTATCAATTGGAGAATAATCTTTTGCAATTTGATTTGGCTGCGTCTAGATTGGGCTTTAGCAACACGATCTTTGCACGGTTTGAACCCGGACCTTACATATATTATGCATTATCTCTACCAAATAAGTTAAGCTCACGAGGAACAAGGAGTATGTGTTTCTTTGATAATCGGAACAGGTTTCTGATGAAATTGAACAACGAAACCGTATCGATCGAGCTCAAGAACGGTACAATTGTTCATGGCACCATTACAGGTGTTGATATCAGCATGAACACACATTTGAAAACAGTTAAACTAACACTGAAGGGGAAAAACCCAGTGACTCTGGATCATCTCAGTGTGAGGGGTAACAACATTCGTTATTATATCCTTCCTGACAGCTTGAATCTTGAGACTTTGCTGGTTGAAGAGGCACCTAGGGTCAAGCCTAAGAAGCCAACTGCTGGTACTTTACATTATCTGACTTGCTGCAATTTTTATTCTGGTTGGCTGCCATCTGGTTTTTGGTTTTCAACGTTTTAA

Protein sequence

>MsG0180003991.01.T01

MAGLGRTKIALPSQLASAFSFERKFAFCFSSSDGVIIFGDGPYSFLADNASLPNVVFDSKSFTYTPLLINPVSTASAFLQGEPSVEYFIGVKTIKIDGKVVSLNSSLLSIDNKGTPLGAAVPTIELVLQNNVIWSMFGANSMVNINDEVLCLGFVNGGENARTSIVIGGYQLENNLLQFDLAASRLGFSNTIFARFEPGPYIYYALSLPNKLSSRGTRSMCFFDNRNRFLMKLNNETVSIELKNGTIVHGTITGVDISMNTHLKTVKLTLKGKNPVTLDHLSVRGNNIRYYILPDSLNLETLLVEEAPRVKPKKPTAGTLHYLTCCNFYSGWLPSGFWFSTF*