Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030994.01.T01 | KEH25125.1 | 89.136 | 359 | 39 | 0 | 1 | 359 | 1 | 359 | 0 | 661 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030994.01.T01 | Q9SCZ4 | 41.525 | 354 | 175 | 12 | 18 | 359 | 21 | 354 | 6.08E-68 | 232 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030994.01.T01 | A0A072UH49 | 89.136 | 359 | 39 | 0 | 1 | 359 | 1 | 359 | 0.0 | 661 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001119.01 | MsG0680030994.01 | 0.807452 | 5.019328e-50 | 2.796818e-47 |
| MsG0180001120.01 | MsG0680030994.01 | 0.814788 | 1.289005e-51 | 8.730979e-49 |
| MsG0180001442.01 | MsG0680030994.01 | 0.803231 | 3.847782e-49 | 1.923294e-46 |
| MsG0180001590.01 | MsG0680030994.01 | 0.838435 | 2.880299e-57 | 3.857738e-54 |
| MsG0180002088.01 | MsG0680030994.01 | 0.864572 | 1.104495e-64 | 3.483892e-61 |
| MsG0180003991.01 | MsG0680030994.01 | -0.800120 | 1.672645e-48 | 7.725658e-46 |
| MsG0180004112.01 | MsG0680030994.01 | 0.802864 | 4.583211e-49 | 2.269602e-46 |
| MsG0180005772.01 | MsG0680030994.01 | -0.818977 | 1.478721e-52 | 1.123645e-49 |
| MsG0280010645.01 | MsG0680030994.01 | 0.822713 | 2.044135e-53 | 1.724424e-50 |
| MsG0280011272.01 | MsG0680030994.01 | -0.813661 | 2.286638e-51 | 1.502327e-48 |
| MsG0280011309.01 | MsG0680030994.01 | 0.812824 | 3.490793e-51 | 2.242002e-48 |
| MsG0380011839.01 | MsG0680030994.01 | 0.820346 | 7.201348e-53 | 5.683536e-50 |
| MsG0380012249.01 | MsG0680030994.01 | 0.826717 | 2.327105e-54 | 2.199757e-51 |
| MsG0380012343.01 | MsG0680030994.01 | 0.800826 | 1.200993e-48 | 5.647412e-46 |
| MsG0680030994.01 | MsG0680031996.01 | 0.803908 | 2.784628e-49 | 1.416427e-46 |
| MsG0680030994.01 | MsG0680032279.01 | 0.820459 | 6.784988e-53 | 5.371934e-50 |
| MsG0680030994.01 | MsG0680032305.01 | 0.802544 | 5.334893e-49 | 2.620364e-46 |
| MsG0680030994.01 | MsG0680032735.01 | 0.800641 | 1.310325e-48 | 6.132767e-46 |
| MsG0680030994.01 | MsG0680033465.01 | 0.835222 | 1.902070e-56 | 2.311143e-53 |
| MsG0680030994.01 | MsG0680034032.01 | 0.803108 | 4.080365e-49 | 2.033069e-46 |
| MsG0680030994.01 | MsG0780036509.01 | 0.807154 | 5.805715e-50 | 3.209888e-47 |
| MsG0680030994.01 | MsG0780037679.01 | 0.806267 | 8.939733e-50 | 4.831278e-47 |
| MsG0680030994.01 | MsG0780039218.01 | 0.838623 | 2.576965e-57 | 3.471243e-54 |
| MsG0680030994.01 | MsG0780039573.01 | 0.803491 | 3.398643e-49 | 1.710260e-46 |
| MsG0680030994.01 | MsG0780039711.01 | 0.819718 | 1.002378e-52 | 7.774293e-50 |
| MsG0680030994.01 | MsG0780040285.01 | 0.827269 | 1.717064e-54 | 1.649629e-51 |
| MsG0680030994.01 | MsG0880044705.01 | 0.843262 | 1.561463e-58 | 2.429526e-55 |
| MsG0680030994.01 | MsG0880045771.01 | 0.804116 | 2.521356e-49 | 1.289313e-46 |
| MsG0680030994.01 | MsG0880047368.01 | 0.825077 | 5.705613e-54 | 5.147754e-51 |
| MsG0480022802.01 | MsG0680030994.01 | 0.808215 | 3.455656e-50 | 1.964371e-47 |
| MsG0580024975.01 | MsG0680030994.01 | 0.858869 | 6.127262e-63 | 1.586898e-59 |
| MsG0380015735.01 | MsG0680030994.01 | 0.827906 | 1.207611e-54 | 1.181798e-51 |
| MsG0580027789.01 | MsG0680030994.01 | 0.806722 | 7.164691e-50 | 3.917211e-47 |
| MsG0580029346.01 | MsG0680030994.01 | 0.831455 | 1.653528e-55 | 1.795342e-52 |
| MsG0580029431.01 | MsG0680030994.01 | 0.801005 | 1.104269e-48 | 5.216731e-46 |
| MsG0680030558.01 | MsG0680030994.01 | 0.813739 | 2.197844e-51 | 1.447052e-48 |
| MsG0280007339.01 | MsG0680030994.01 | 0.803705 | 3.068265e-49 | 1.552450e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030994.01.T01 | MTR_6g015795 | 89.136 | 359 | 39 | 0 | 1 | 359 | 1 | 359 | 0.0 | 661 |
| MsG0680030994.01.T01 | MTR_6g015805 | 87.744 | 359 | 38 | 3 | 1 | 359 | 1 | 353 | 0.0 | 638 |
| MsG0680030994.01.T01 | MTR_5g047110 | 38.904 | 365 | 205 | 11 | 1 | 358 | 2 | 355 | 1.62e-66 | 224 |
| MsG0680030994.01.T01 | MTR_4g111925 | 38.781 | 361 | 187 | 11 | 11 | 359 | 16 | 354 | 3.75e-65 | 219 |
| MsG0680030994.01.T01 | MTR_7g073660 | 38.781 | 361 | 187 | 11 | 11 | 359 | 16 | 354 | 4.01e-64 | 219 |
| MsG0680030994.01.T01 | MTR_4g052290 | 39.459 | 370 | 198 | 14 | 7 | 359 | 5 | 365 | 6.30e-63 | 216 |
| MsG0680030994.01.T01 | MTR_5g047060 | 37.466 | 363 | 209 | 10 | 1 | 355 | 2 | 354 | 3.03e-62 | 213 |
| MsG0680030994.01.T01 | MTR_7g015670 | 40.173 | 346 | 187 | 13 | 26 | 359 | 31 | 368 | 1.09e-61 | 212 |
| MsG0680030994.01.T01 | MTR_7g015310 | 41.033 | 368 | 184 | 16 | 12 | 359 | 13 | 367 | 2.92e-61 | 211 |
| MsG0680030994.01.T01 | MTR_7g015250 | 39.437 | 355 | 184 | 15 | 26 | 359 | 29 | 373 | 2.64e-60 | 209 |
| MsG0680030994.01.T01 | MTR_7g015240 | 41.061 | 358 | 166 | 15 | 26 | 359 | 29 | 365 | 4.59e-60 | 207 |
| MsG0680030994.01.T01 | MTR_7g015390 | 40.057 | 352 | 182 | 15 | 26 | 359 | 29 | 369 | 1.41e-59 | 206 |
| MsG0680030994.01.T01 | MTR_7g015550 | 38.920 | 352 | 184 | 13 | 26 | 359 | 31 | 369 | 4.02e-56 | 197 |
| MsG0680030994.01.T01 | MTR_7g015510 | 38.095 | 357 | 180 | 13 | 26 | 359 | 31 | 369 | 2.66e-55 | 194 |
| MsG0680030994.01.T01 | MTR_7g015280 | 37.784 | 352 | 179 | 14 | 26 | 359 | 29 | 358 | 1.38e-54 | 192 |
| MsG0680030994.01.T01 | MTR_7g015620 | 38.177 | 351 | 192 | 15 | 24 | 359 | 23 | 363 | 6.38e-54 | 188 |
| MsG0680030994.01.T01 | MTR_7g015320 | 38.244 | 353 | 194 | 13 | 26 | 359 | 29 | 376 | 7.14e-54 | 190 |
| MsG0680030994.01.T01 | MTR_7g015230 | 38.123 | 341 | 194 | 11 | 31 | 359 | 37 | 372 | 7.63e-54 | 190 |
| MsG0680030994.01.T01 | MTR_7g015420 | 38.218 | 348 | 188 | 15 | 26 | 359 | 29 | 363 | 7.13e-52 | 185 |
| MsG0680030994.01.T01 | MTR_1g080740 | 32.213 | 357 | 216 | 13 | 8 | 359 | 5 | 340 | 1.42e-38 | 146 |
| MsG0680030994.01.T01 | MTR_5g047120 | 30.435 | 345 | 202 | 11 | 35 | 359 | 41 | 367 | 5.59e-38 | 145 |
| MsG0680030994.01.T01 | MTR_7g115300 | 33.239 | 355 | 209 | 14 | 14 | 359 | 15 | 350 | 9.78e-37 | 141 |
| MsG0680030994.01.T01 | MTR_2g030310 | 26.005 | 373 | 236 | 13 | 1 | 359 | 2 | 348 | 1.66e-28 | 117 |
| MsG0680030994.01.T01 | MTR_2g030310 | 26.005 | 373 | 236 | 13 | 1 | 359 | 2 | 348 | 1.79e-28 | 117 |
| MsG0680030994.01.T01 | MTR_6g048090 | 30.460 | 348 | 200 | 14 | 26 | 359 | 22 | 341 | 7.08e-28 | 115 |
| MsG0680030994.01.T01 | MTR_2g080220 | 29.878 | 328 | 204 | 11 | 27 | 353 | 27 | 329 | 3.48e-26 | 110 |
| MsG0680030994.01.T01 | MTR_2g096160 | 29.129 | 333 | 199 | 15 | 10 | 332 | 10 | 315 | 3.87e-25 | 107 |
| MsG0680030994.01.T01 | MTR_7g078220 | 28.134 | 359 | 217 | 16 | 11 | 359 | 9 | 336 | 1.96e-24 | 104 |
| MsG0680030994.01.T01 | MTR_4g061833 | 28.319 | 339 | 210 | 14 | 27 | 359 | 28 | 339 | 4.38e-23 | 101 |
| MsG0680030994.01.T01 | MTR_4g061930 | 28.319 | 339 | 210 | 14 | 27 | 359 | 28 | 339 | 4.38e-23 | 101 |
| MsG0680030994.01.T01 | MTR_1g100110 | 26.000 | 350 | 207 | 16 | 26 | 359 | 37 | 350 | 1.61e-22 | 99.4 |
| MsG0680030994.01.T01 | MTR_4g109010 | 29.965 | 287 | 160 | 14 | 85 | 359 | 105 | 362 | 2.27e-22 | 99.4 |
| MsG0680030994.01.T01 | MTR_8g467150 | 26.593 | 361 | 221 | 15 | 1 | 355 | 3 | 325 | 4.15e-21 | 95.1 |
| MsG0680030994.01.T01 | MTR_8g037700 | 28.818 | 347 | 197 | 18 | 27 | 359 | 59 | 369 | 5.53e-20 | 92.0 |
| MsG0680030994.01.T01 | MTR_4g095042 | 25.770 | 357 | 203 | 18 | 5 | 335 | 3 | 323 | 2.07e-15 | 78.2 |
| MsG0680030994.01.T01 | MTR_4g095018 | 26.496 | 351 | 209 | 19 | 6 | 335 | 5 | 327 | 4.22e-15 | 77.0 |
| MsG0680030994.01.T01 | MTR_5g047070 | 44.737 | 76 | 41 | 1 | 285 | 359 | 51 | 126 | 1.46e-13 | 72.0 |
| MsG0680030994.01.T01 | MTR_4g061833 | 29.327 | 208 | 123 | 10 | 156 | 359 | 1 | 188 | 4.56e-13 | 70.9 |
| MsG0680030994.01.T01 | MTR_4g095032 | 24.551 | 334 | 217 | 16 | 12 | 332 | 7 | 318 | 2.29e-12 | 68.6 |
| MsG0680030994.01.T01 | MTR_4g095012 | 24.332 | 337 | 215 | 15 | 10 | 332 | 9 | 319 | 3.95e-12 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030994.01.T01 | AT3G51550 | 41.525 | 354 | 175 | 12 | 18 | 359 | 21 | 354 | 6.20e-69 | 232 |
| MsG0680030994.01.T01 | AT5G39000 | 38.053 | 339 | 189 | 11 | 27 | 359 | 26 | 349 | 1.27e-58 | 204 |
| MsG0680030994.01.T01 | AT5G39020 | 36.686 | 338 | 194 | 11 | 26 | 359 | 22 | 343 | 1.95e-58 | 202 |
| MsG0680030994.01.T01 | AT5G38990 | 38.227 | 361 | 203 | 14 | 4 | 359 | 1 | 346 | 8.25e-58 | 201 |
| MsG0680030994.01.T01 | AT5G39030 | 36.723 | 354 | 205 | 10 | 11 | 359 | 5 | 344 | 4.08e-54 | 191 |
| MsG0680030994.01.T01 | AT5G39030 | 37.574 | 338 | 192 | 10 | 27 | 359 | 15 | 338 | 1.30e-53 | 189 |
| MsG0680030994.01.T01 | AT5G28680 | 31.322 | 348 | 217 | 11 | 10 | 354 | 7 | 335 | 1.06e-39 | 150 |
| MsG0680030994.01.T01 | AT5G28680 | 31.322 | 348 | 217 | 11 | 10 | 354 | 7 | 335 | 1.06e-39 | 150 |
| MsG0680030994.01.T01 | AT3G04690 | 33.333 | 327 | 194 | 12 | 35 | 358 | 31 | 336 | 1.76e-39 | 149 |
| MsG0680030994.01.T01 | AT5G59700 | 28.280 | 343 | 196 | 12 | 11 | 344 | 9 | 310 | 1.05e-29 | 120 |
| MsG0680030994.01.T01 | AT1G30570 | 26.287 | 369 | 231 | 15 | 7 | 359 | 9 | 352 | 1.43e-28 | 117 |
| MsG0680030994.01.T01 | AT4G00300 | 28.177 | 362 | 226 | 18 | 5 | 359 | 3 | 337 | 1.18e-27 | 113 |
| MsG0680030994.01.T01 | AT3G46290 | 28.375 | 363 | 209 | 14 | 7 | 359 | 6 | 327 | 1.36e-27 | 114 |
| MsG0680030994.01.T01 | AT5G61350 | 27.114 | 343 | 191 | 16 | 26 | 348 | 27 | 330 | 9.13e-24 | 103 |
| MsG0680030994.01.T01 | AT5G54380 | 28.571 | 357 | 224 | 14 | 8 | 359 | 5 | 335 | 2.60e-23 | 102 |
| MsG0680030994.01.T01 | AT2G21480 | 27.729 | 339 | 203 | 18 | 26 | 353 | 50 | 357 | 6.89e-23 | 100 |
| MsG0680030994.01.T01 | AT5G24010 | 28.834 | 326 | 205 | 14 | 13 | 332 | 10 | 314 | 2.78e-21 | 95.9 |
| MsG0680030994.01.T01 | AT2G39360 | 29.480 | 346 | 186 | 18 | 4 | 334 | 1 | 303 | 1.20e-20 | 94.0 |
| MsG0680030994.01.T01 | AT4G39110 | 27.826 | 345 | 195 | 20 | 26 | 353 | 51 | 358 | 4.94e-20 | 92.0 |
| MsG0680030994.01.T01 | AT2G23200 | 27.654 | 358 | 203 | 15 | 7 | 350 | 13 | 328 | 4.91e-18 | 86.3 |
Find 84 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGAAATTCTAATAATTA+TGG | 0.216412 | 6:-12650002 | MsG0680030994.01.T01:CDS |
| TGAATATGGAACTTTGTTTA+TGG | 0.228332 | 6:+12650295 | None:intergenic |
| TGATGTTGGTGTTCTTAAAT+AGG | 0.277271 | 6:+12648858 | None:intergenic |
| TGGGAATTCCCAGTTAATTC+TGG | 0.277658 | 6:-12648697 | MsG0680030994.01.T01:CDS |
| ACATAGGCATGCTCAGAAAT+TGG | 0.279800 | 6:-12648897 | MsG0680030994.01.T01:CDS |
| GTATAAGAGGGTGATGATTA+TGG | 0.300832 | 6:+12650505 | None:intergenic |
| AAATAGTTTCACACTTCTTA+AGG | 0.312306 | 6:-12650135 | MsG0680030994.01.T01:CDS |
| GGAATTCCCAAGTTAGGTTT+AGG | 0.316204 | 6:+12648710 | None:intergenic |
| CTCGTACCTGTTATATCTGC+TGG | 0.324909 | 6:+12648823 | None:intergenic |
| TAACTGGGAATTCCCAAGTT+AGG | 0.346758 | 6:+12648704 | None:intergenic |
| TCCTTTGGTGCTATGTAGTC+AGG | 0.359188 | 6:+12648784 | None:intergenic |
| AACCTCAATTGCGGTTCAAC+AGG | 0.370073 | 6:-12650431 | MsG0680030994.01.T01:CDS |
| TTATTAGAATTTCCACTACT+TGG | 0.372361 | 6:+12650008 | None:intergenic |
| CTGGCTTCACTTACTTCATC+AGG | 0.383397 | 6:-12648678 | MsG0680030994.01.T01:CDS |
| CACATTTCCTTGTAGATAAA+CGG | 0.392473 | 6:+12648600 | None:intergenic |
| TAAGTGAAGCCAGAATTAAC+TGG | 0.395914 | 6:+12648688 | None:intergenic |
| GATAAACGTGCTGATGAATA+TGG | 0.399120 | 6:+12650281 | None:intergenic |
| ACAAACTCCTAAACCTAACT+TGG | 0.401153 | 6:-12648717 | MsG0680030994.01.T01:CDS |
| AAACCGTTGGTATAAGTAGT+AGG | 0.406261 | 6:+12650191 | None:intergenic |
| AAGTCGACCCGAACATAACT+AGG | 0.416722 | 6:-12648642 | MsG0680030994.01.T01:CDS |
| GGGAGGTGGAAAAGTATAAG+AGG | 0.417418 | 6:+12650492 | None:intergenic |
| GGTGTATAGTATAGATCATC+AGG | 0.423919 | 6:+12649948 | None:intergenic |
| TTTGGAACAGAAATTGTTGT+TGG | 0.429833 | 6:+12650335 | None:intergenic |
| TTGAGGTTAAAACGGTCAAT+TGG | 0.437194 | 6:+12650446 | None:intergenic |
| CGTAGGCTTGAAGGTGTAAG+AGG | 0.448967 | 6:+12650471 | None:intergenic |
| GTTTAGGAGTTTGTTGAGAG+TGG | 0.450722 | 6:+12648726 | None:intergenic |
| ATGCCTATGTCTGGAATGTT+TGG | 0.450998 | 6:+12648910 | None:intergenic |
| CAATTGGTTCGTAGGCTTGA+AGG | 0.460075 | 6:+12650462 | None:intergenic |
| TCGGGTCGACTTCACAGAAA+TGG | 0.462203 | 6:+12648653 | None:intergenic |
| TCGGCCGCCCTAGTTATGTT+CGG | 0.464490 | 6:+12648634 | None:intergenic |
| AAAGAATATGTTATTAACGT+TGG | 0.468220 | 6:-12650062 | MsG0680030994.01.T01:CDS |
| TTTCTGAGCATGCCTATGTC+TGG | 0.468730 | 6:+12648901 | None:intergenic |
| TGTGAAACTATTTGAAATAA+CGG | 0.472819 | 6:+12650145 | None:intergenic |
| GAGTTTGTTGAGAGTGGCAT+TGG | 0.479609 | 6:+12648732 | None:intergenic |
| TTCGTCCCAGCAGATATAAC+AGG | 0.480575 | 6:-12648829 | MsG0680030994.01.T01:CDS |
| GATGATTTGAAGAGGTCCTT+TGG | 0.480613 | 6:+12648769 | None:intergenic |
| TATACACCACCTGAGGATCC+TGG | 0.485685 | 6:-12649933 | MsG0680030994.01.T01:CDS |
| TCATTAAACGCTGATGTTGA+AGG | 0.490768 | 6:-12650101 | MsG0680030994.01.T01:CDS |
| TATGGAACTTTGTTTATGGA+AGG | 0.492218 | 6:+12650299 | None:intergenic |
| ACCTGTCTCTAGTGCAACAC+TGG | 0.497136 | 6:+12649863 | None:intergenic |
| AACCGCAATTGAGGTTAAAA+CGG | 0.500267 | 6:+12650438 | None:intergenic |
| GTCTTCACCGTTTATCTACA+AGG | 0.504270 | 6:-12648607 | MsG0680030994.01.T01:CDS |
| ATCATAACGGTTAAAACCGT+TGG | 0.507788 | 6:+12650178 | None:intergenic |
| CGGCCGCCCTAGTTATGTTC+GGG | 0.516085 | 6:+12648635 | None:intergenic |
| AAAACGGTCAATTGGTTCGT+AGG | 0.516426 | 6:+12650454 | None:intergenic |
| ACCAGTGTTGCACTAGAGAC+AGG | 0.517626 | 6:-12649864 | MsG0680030994.01.T01:intron |
| TTAACGTTGGTGATGATCAA+AGG | 0.525996 | 6:-12650049 | MsG0680030994.01.T01:CDS |
| TGTCCATGTTCTTTGTCCGT+CGG | 0.529392 | 6:+12650400 | None:intergenic |
| AGTGAAAATCCAGGATCCTC+AGG | 0.532582 | 6:+12649924 | None:intergenic |
| TTCAAATCATCACGTGACTT+AGG | 0.533902 | 6:-12648757 | MsG0680030994.01.T01:CDS |
| TACCCTACTACTTATACCAA+CGG | 0.537022 | 6:-12650194 | MsG0680030994.01.T01:CDS |
| GACGGACAAAGAACATGGAC+AGG | 0.537852 | 6:-12650398 | MsG0680030994.01.T01:CDS |
| TATTCTTTACCCGTCAGTAC+AGG | 0.538429 | 6:-12650242 | MsG0680030994.01.T01:CDS |
| GTCTCTAGTGCAACACTGGT+TGG | 0.544929 | 6:+12649867 | None:intergenic |
| TCGTACCTGTTATATCTGCT+GGG | 0.545233 | 6:+12648824 | None:intergenic |
| AACCGTTGGTATAAGTAGTA+GGG | 0.547927 | 6:+12650192 | None:intergenic |
| TTCCTGTTGAACCGCAATTG+AGG | 0.548480 | 6:+12650429 | None:intergenic |
| TTGAAGGTGTAAGAGGGAGG+TGG | 0.551545 | 6:+12650478 | None:intergenic |
| GGAGGTGGAAAAGTATAAGA+GGG | 0.563249 | 6:+12650493 | None:intergenic |
| CTACCGACGGACAAAGAACA+TGG | 0.572671 | 6:-12650403 | MsG0680030994.01.T01:CDS |
| TCACCAAACATTCCAGACAT+AGG | 0.585221 | 6:-12648913 | MsG0680030994.01.T01:CDS |
| AATAGTATATTGTGGTATGA+TGG | 0.586058 | 6:+12649890 | None:intergenic |
| ACCTGACTACATAGCACCAA+AGG | 0.586619 | 6:-12648785 | MsG0680030994.01.T01:CDS |
| GACCCCACAAGTGAAAATCC+AGG | 0.594772 | 6:+12649915 | None:intergenic |
| CGTTTATCTACAAGGAAATG+TGG | 0.603064 | 6:-12648599 | MsG0680030994.01.T01:CDS |
| GGGACGAAGTCACGTGATGT+TGG | 0.604906 | 6:+12648844 | None:intergenic |
| GAATTTAGGACCTGTACTGA+CGG | 0.605805 | 6:+12650232 | None:intergenic |
| CAAACTCCTAAACCTAACTT+GGG | 0.620904 | 6:-12648716 | MsG0680030994.01.T01:CDS |
| AAGTGAAGCCAGAATTAACT+GGG | 0.620963 | 6:+12648689 | None:intergenic |
| AATTTAGGACCTGTACTGAC+GGG | 0.621399 | 6:+12650233 | None:intergenic |
| ATAGTATAGATCATCAGGCA+TGG | 0.630000 | 6:+12649953 | None:intergenic |
| AGTATATTGTGGTATGATGG+TGG | 0.637395 | 6:+12649893 | None:intergenic |
| AGTCACGTGATGATTTGAAG+AGG | 0.649942 | 6:+12648761 | None:intergenic |
| GTAGGCTTGAAGGTGTAAGA+GGG | 0.650988 | 6:+12650472 | None:intergenic |
| GCAGATTACAGAATCAAAGT+CGG | 0.651473 | 6:-12648949 | MsG0680030994.01.T01:intron |
| GGCTTGAAGGTGTAAGAGGG+AGG | 0.658222 | 6:+12650475 | None:intergenic |
| AGTCGACCCGAACATAACTA+GGG | 0.671905 | 6:-12648641 | MsG0680030994.01.T01:CDS |
| TCAACAGGAAACTCTACCGA+CGG | 0.672249 | 6:-12650416 | MsG0680030994.01.T01:CDS |
| ATAAACGGTGAAGACTCTAT+CGG | 0.674255 | 6:+12648615 | None:intergenic |
| GAAAATCCAGGATCCTCAGG+TGG | 0.679128 | 6:+12649927 | None:intergenic |
| CTGGTTGGAATAGTATATTG+TGG | 0.691001 | 6:+12649882 | None:intergenic |
| TCTATACTATACACCACCTG+AGG | 0.712933 | 6:-12649940 | MsG0680030994.01.T01:CDS |
| CAGCAGATATAACAGGTACG+AGG | 0.714901 | 6:-12648822 | MsG0680030994.01.T01:CDS |
| CGACCCGAACATAACTAGGG+CGG | 0.722973 | 6:-12648638 | MsG0680030994.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAATTATGGTTTTATTAA+TGG | - | Chr6:12649116-12649135 | MsG0680030994.01.T01:intron | 10.0% |
| !!! | CAATAAATAATTTTAATCTT+TGG | + | Chr6:12649395-12649414 | None:intergenic | 10.0% |
| !! | CAAAATTCAATTCAAAAATT+TGG | + | Chr6:12649605-12649624 | None:intergenic | 15.0% |
| !! | TATATGTTTATAGGATTTAA+AGG | + | Chr6:12650067-12650086 | None:intergenic | 15.0% |
| !!! | TAATCCTGAATTTTTATTAT+TGG | + | Chr6:12649630-12649649 | None:intergenic | 15.0% |
| !!! | TATATATTGTTATTGTTTGT+TGG | - | Chr6:12649812-12649831 | MsG0680030994.01.T01:intron | 15.0% |
| !! | AAAGAATATGTTATTAACGT+TGG | - | Chr6:12649044-12649063 | MsG0680030994.01.T01:intron | 20.0% |
| !! | AGTGGAAATTCTAATAATTA+TGG | - | Chr6:12649104-12649123 | MsG0680030994.01.T01:intron | 20.0% |
| !! | ATTAGCTAGATTAATGATTA+TGG | - | Chr6:12649696-12649715 | MsG0680030994.01.T01:intron | 20.0% |
| !! | TGATCCAATAATAAAAATTC+AGG | - | Chr6:12649623-12649642 | MsG0680030994.01.T01:intron | 20.0% |
| !!! | TGTGAAACTATTTGAAATAA+CGG | + | Chr6:12648964-12648983 | None:intergenic | 20.0% |
| ! | AAATAGTTTCACACTTCTTA+AGG | - | Chr6:12648971-12648990 | MsG0680030994.01.T01:intron | 25.0% |
| ! | AATAGTATATTGTGGTATGA+TGG | + | Chr6:12649219-12649238 | None:intergenic | 25.0% |
| ! | AATGCCATCCTATTATATAT+TGG | - | Chr6:12650090-12650109 | MsG0680030994.01.T01:CDS | 25.0% |
| ! | ATATATAATAGGATGGCATT+AGG | + | Chr6:12650090-12650109 | None:intergenic | 25.0% |
| ! | ATTGTTTAACATATCTCCTT+AGG | + | Chr6:12649852-12649871 | None:intergenic | 25.0% |
| ! | ATTTAAAGGAAATCCATTTG+TGG | + | Chr6:12650053-12650072 | None:intergenic | 25.0% |
| ! | GAATGCAACCAATATATAAT+AGG | + | Chr6:12650101-12650120 | None:intergenic | 25.0% |
| ! | TCTTAACATGTGTCATATAA+AGG | + | Chr6:12649370-12649389 | None:intergenic | 25.0% |
| ! | TTATTAGAATTTCCACTACT+TGG | + | Chr6:12649101-12649120 | None:intergenic | 25.0% |
| ! | TTTAAAGGAAATCCATTTGT+GGG | + | Chr6:12650052-12650071 | None:intergenic | 25.0% |
| ! | TTTAGTCATATCAGAGATAT+CGG | + | Chr6:12649528-12649547 | None:intergenic | 25.0% |
| !! | TGAATATGGAACTTTGTTTA+TGG | + | Chr6:12648814-12648833 | None:intergenic | 25.0% |
| !!! | AGATATCGGACAATATTTTT+TGG | + | Chr6:12649514-12649533 | None:intergenic | 25.0% |
| !!! | ATGGTTTTGAAGTTTATCAA+TGG | + | Chr6:12648585-12648604 | None:intergenic | 25.0% |
| AAAAAAAGACGGAGGAATTT+AGG | + | Chr6:12648891-12648910 | None:intergenic | 30.0% | |
| CACATTTCCTTGTAGATAAA+CGG | + | Chr6:12650509-12650528 | None:intergenic | 30.0% | |
| GCAACCAATATATAATAGGA+TGG | + | Chr6:12650097-12650116 | None:intergenic | 30.0% | |
| GGCATTAGGTATATGTTTAT+AGG | + | Chr6:12650076-12650095 | None:intergenic | 30.0% | |
| GTGTGAATAAGTGAAAATTG+CGG | - | Chr6:12649970-12649989 | MsG0680030994.01.T01:CDS | 30.0% | |
| TAGTAGGGTAGAAAAAAAGA+CGG | + | Chr6:12648902-12648921 | None:intergenic | 30.0% | |
| TGTGAATAAGTGAAAATTGC+GGG | - | Chr6:12649971-12649990 | MsG0680030994.01.T01:CDS | 30.0% | |
| TTTGATTCTGTAATCTGCAA+TGG | + | Chr6:12650155-12650174 | None:intergenic | 30.0% | |
| ! | GAAGGATTTTGTGTCTTTTT+TGG | + | Chr6:12648792-12648811 | None:intergenic | 30.0% |
| ! | TATGGAACTTTGTTTATGGA+AGG | + | Chr6:12648810-12648829 | None:intergenic | 30.0% |
| ! | TGATGTTGGTGTTCTTAAAT+AGG | + | Chr6:12650251-12650270 | None:intergenic | 30.0% |
| ! | TTTGGAACAGAAATTGTTGT+TGG | + | Chr6:12648774-12648793 | None:intergenic | 30.0% |
| !! | CGGTAAAAAAAGCATCATAA+CGG | + | Chr6:12648944-12648963 | None:intergenic | 30.0% |
| !!! | TTGAGTTTTATTCCAAGTAG+TGG | - | Chr6:12649086-12649105 | MsG0680030994.01.T01:intron | 30.0% |
| AAACCGTTGGTATAAGTAGT+AGG | + | Chr6:12648918-12648937 | None:intergenic | 35.0% | |
| AACCGCAATTGAGGTTAAAA+CGG | + | Chr6:12648671-12648690 | None:intergenic | 35.0% | |
| AACCGTTGGTATAAGTAGTA+GGG | + | Chr6:12648917-12648936 | None:intergenic | 35.0% | |
| AAGTGAAGCCAGAATTAACT+GGG | + | Chr6:12650420-12650439 | None:intergenic | 35.0% | |
| ACAAACTCCTAAACCTAACT+TGG | - | Chr6:12650389-12650408 | MsG0680030994.01.T01:CDS | 35.0% | |
| AGTATATTGTGGTATGATGG+TGG | + | Chr6:12649216-12649235 | None:intergenic | 35.0% | |
| ATAAACGGTGAAGACTCTAT+CGG | + | Chr6:12650494-12650513 | None:intergenic | 35.0% | |
| ATAGTATAGATCATCAGGCA+TGG | + | Chr6:12649156-12649175 | None:intergenic | 35.0% | |
| ATCATAACGGTTAAAACCGT+TGG | + | Chr6:12648931-12648950 | None:intergenic | 35.0% | |
| ATGTTTGATGTGTTGAAACG+CGG | + | Chr6:12650004-12650023 | None:intergenic | 35.0% | |
| CAAACTCCTAAACCTAACTT+GGG | - | Chr6:12650390-12650409 | MsG0680030994.01.T01:CDS | 35.0% | |
| CGTTTATCTACAAGGAAATG+TGG | - | Chr6:12650507-12650526 | MsG0680030994.01.T01:CDS | 35.0% | |
| CTGGTTGGAATAGTATATTG+TGG | + | Chr6:12649227-12649246 | None:intergenic | 35.0% | |
| GATACACACATTCATCTAGT+AGG | + | Chr6:12649580-12649599 | None:intergenic | 35.0% | |
| GCAGATTACAGAATCAAAGT+CGG | - | Chr6:12650157-12650176 | MsG0680030994.01.T01:CDS | 35.0% | |
| GGTGTATAGTATAGATCATC+AGG | + | Chr6:12649161-12649180 | None:intergenic | 35.0% | |
| GGTTCATGATAACTAACCTA+AGG | - | Chr6:12649833-12649852 | MsG0680030994.01.T01:intron | 35.0% | |
| GTATAAGAGGGTGATGATTA+TGG | + | Chr6:12648604-12648623 | None:intergenic | 35.0% | |
| TAAGTGAAGCCAGAATTAAC+TGG | + | Chr6:12650421-12650440 | None:intergenic | 35.0% | |
| TACCCTACTACTTATACCAA+CGG | - | Chr6:12648912-12648931 | MsG0680030994.01.T01:CDS | 35.0% | |
| TCATTAAACGCTGATGTTGA+AGG | - | Chr6:12649005-12649024 | MsG0680030994.01.T01:intron | 35.0% | |
| TTCAAATCATCACGTGACTT+AGG | - | Chr6:12650349-12650368 | MsG0680030994.01.T01:CDS | 35.0% | |
| TTGAGGTTAAAACGGTCAAT+TGG | + | Chr6:12648663-12648682 | None:intergenic | 35.0% | |
| ! | GATAAACGTGCTGATGAATA+TGG | + | Chr6:12648828-12648847 | None:intergenic | 35.0% |
| ! | TAGTAGGATGCTTGTTAGTA+AGG | + | Chr6:12649564-12649583 | None:intergenic | 35.0% |
| !! | TTAACGTTGGTGATGATCAA+AGG | - | Chr6:12649057-12649076 | MsG0680030994.01.T01:intron | 35.0% |
| AACTGAGTTGTACCCACAAA+TGG | - | Chr6:12650037-12650056 | MsG0680030994.01.T01:CDS | 40.0% | |
| ACATAGGCATGCTCAGAAAT+TGG | - | Chr6:12650209-12650228 | MsG0680030994.01.T01:CDS | 40.0% | |
| AGTCACGTGATGATTTGAAG+AGG | + | Chr6:12650348-12650367 | None:intergenic | 40.0% | |
| GATGATTTGAAGAGGTCCTT+TGG | + | Chr6:12650340-12650359 | None:intergenic | 40.0% | |
| GGAATTCCCAAGTTAGGTTT+AGG | + | Chr6:12650399-12650418 | None:intergenic | 40.0% | |
| GTCTTCACCGTTTATCTACA+AGG | - | Chr6:12650499-12650518 | MsG0680030994.01.T01:CDS | 40.0% | |
| TAACTGGGAATTCCCAAGTT+AGG | + | Chr6:12650405-12650424 | None:intergenic | 40.0% | |
| TAGGGTAGAAAAAAAGACGG+AGG | + | Chr6:12648899-12648918 | None:intergenic | 40.0% | |
| TATTCTTTACCCGTCAGTAC+AGG | - | Chr6:12648864-12648883 | MsG0680030994.01.T01:CDS | 40.0% | |
| TCACCAAACATTCCAGACAT+AGG | - | Chr6:12650193-12650212 | MsG0680030994.01.T01:CDS | 40.0% | |
| TCGTACCTGTTATATCTGCT+GGG | + | Chr6:12650285-12650304 | None:intergenic | 40.0% | |
| TCTATACTATACACCACCTG+AGG | - | Chr6:12649166-12649185 | MsG0680030994.01.T01:intron | 40.0% | |
| TGGGAATTCCCAGTTAATTC+TGG | - | Chr6:12650409-12650428 | MsG0680030994.01.T01:CDS | 40.0% | |
| ! | AATTTAGGACCTGTACTGAC+GGG | + | Chr6:12648876-12648895 | None:intergenic | 40.0% |
| ! | ATCCTGGATTTTCACTTGTG+GGG | - | Chr6:12649189-12649208 | MsG0680030994.01.T01:intron | 40.0% |
| ! | ATGCCTATGTCTGGAATGTT+TGG | + | Chr6:12650199-12650218 | None:intergenic | 40.0% |
| ! | GAATTTAGGACCTGTACTGA+CGG | + | Chr6:12648877-12648896 | None:intergenic | 40.0% |
| ! | GATCCTGGATTTTCACTTGT+GGG | - | Chr6:12649188-12649207 | MsG0680030994.01.T01:intron | 40.0% |
| ! | GGAGGTGGAAAAGTATAAGA+GGG | + | Chr6:12648616-12648635 | None:intergenic | 40.0% |
| !! | AAAACGGTCAATTGGTTCGT+AGG | + | Chr6:12648655-12648674 | None:intergenic | 40.0% |
| !! | GACCGTTTTAACCTCAATTG+CGG | - | Chr6:12648666-12648685 | MsG0680030994.01.T01:CDS | 40.0% |
| !! | GTTTAGGAGTTTGTTGAGAG+TGG | + | Chr6:12650383-12650402 | None:intergenic | 40.0% |
| AACCTCAATTGCGGTTCAAC+AGG | - | Chr6:12648675-12648694 | MsG0680030994.01.T01:CDS | 45.0% | |
| AAGTCGACCCGAACATAACT+AGG | - | Chr6:12650464-12650483 | MsG0680030994.01.T01:CDS | 45.0% | |
| ACCTGACTACATAGCACCAA+AGG | - | Chr6:12650321-12650340 | MsG0680030994.01.T01:CDS | 45.0% | |
| AGTCGACCCGAACATAACTA+GGG | - | Chr6:12650465-12650484 | MsG0680030994.01.T01:CDS | 45.0% | |
| AGTGAAAATCCAGGATCCTC+AGG | + | Chr6:12649185-12649204 | None:intergenic | 45.0% | |
| CAGCAGATATAACAGGTACG+AGG | - | Chr6:12650284-12650303 | MsG0680030994.01.T01:CDS | 45.0% | |
| CTCGTACCTGTTATATCTGC+TGG | + | Chr6:12650286-12650305 | None:intergenic | 45.0% | |
| CTGGCTTCACTTACTTCATC+AGG | - | Chr6:12650428-12650447 | MsG0680030994.01.T01:CDS | 45.0% | |
| TCAACAGGAAACTCTACCGA+CGG | - | Chr6:12648690-12648709 | MsG0680030994.01.T01:CDS | 45.0% | |
| TGTCCATGTTCTTTGTCCGT+CGG | + | Chr6:12648709-12648728 | None:intergenic | 45.0% | |
| TTCCTGTTGAACCGCAATTG+AGG | + | Chr6:12648680-12648699 | None:intergenic | 45.0% | |
| TTCGTCCCAGCAGATATAAC+AGG | - | Chr6:12650277-12650296 | MsG0680030994.01.T01:CDS | 45.0% | |
| TTTCTGAGCATGCCTATGTC+TGG | + | Chr6:12650208-12650227 | None:intergenic | 45.0% | |
| ! | GGATCCTGGATTTTCACTTG+TGG | - | Chr6:12649187-12649206 | MsG0680030994.01.T01:intron | 45.0% |
| ! | GGGAGGTGGAAAAGTATAAG+AGG | + | Chr6:12648617-12648636 | None:intergenic | 45.0% |
| ! | GTAGGCTTGAAGGTGTAAGA+GGG | + | Chr6:12648637-12648656 | None:intergenic | 45.0% |
| !! | CAATTGGTTCGTAGGCTTGA+AGG | + | Chr6:12648647-12648666 | None:intergenic | 45.0% |
| !! | GAGTTTGTTGAGAGTGGCAT+TGG | + | Chr6:12650377-12650396 | None:intergenic | 45.0% |
| !! | TCCTTTGGTGCTATGTAGTC+AGG | + | Chr6:12650325-12650344 | None:intergenic | 45.0% |
| !! | AAAATTATTTATTGAAAATT+GGG | - | Chr6:12649401-12649420 | MsG0680030994.01.T01:intron | 5.0% |
| !! | TAAAATTATTTATTGAAAAT+TGG | - | Chr6:12649400-12649419 | MsG0680030994.01.T01:intron | 5.0% |
| ACCAGTGTTGCACTAGAGAC+AGG | - | Chr6:12649242-12649261 | MsG0680030994.01.T01:intron | 50.0% | |
| ACCTGTCTCTAGTGCAACAC+TGG | + | Chr6:12649246-12649265 | None:intergenic | 50.0% | |
| CTACCGACGGACAAAGAACA+TGG | - | Chr6:12648703-12648722 | MsG0680030994.01.T01:CDS | 50.0% | |
| GAAAATCCAGGATCCTCAGG+TGG | + | Chr6:12649182-12649201 | None:intergenic | 50.0% | |
| GACCCCACAAGTGAAAATCC+AGG | + | Chr6:12649194-12649213 | None:intergenic | 50.0% | |
| GACGGACAAAGAACATGGAC+AGG | - | Chr6:12648708-12648727 | MsG0680030994.01.T01:CDS | 50.0% | |
| GTCTCTAGTGCAACACTGGT+TGG | + | Chr6:12649242-12649261 | None:intergenic | 50.0% | |
| TATACACCACCTGAGGATCC+TGG | - | Chr6:12649173-12649192 | MsG0680030994.01.T01:intron | 50.0% | |
| ! | CGTAGGCTTGAAGGTGTAAG+AGG | + | Chr6:12648638-12648657 | None:intergenic | 50.0% |
| ! | TCGGGTCGACTTCACAGAAA+TGG | + | Chr6:12650456-12650475 | None:intergenic | 50.0% |
| ! | TTGAAGGTGTAAGAGGGAGG+TGG | + | Chr6:12648631-12648650 | None:intergenic | 50.0% |
| CGACCCGAACATAACTAGGG+CGG | - | Chr6:12650468-12650487 | MsG0680030994.01.T01:CDS | 55.0% | |
| GGGACGAAGTCACGTGATGT+TGG | + | Chr6:12650265-12650284 | None:intergenic | 55.0% | |
| TCGGCCGCCCTAGTTATGTT+CGG | + | Chr6:12650475-12650494 | None:intergenic | 55.0% | |
| ! | GGCTTGAAGGTGTAAGAGGG+AGG | + | Chr6:12648634-12648653 | None:intergenic | 55.0% |
| CGGCCGCCCTAGTTATGTTC+GGG | + | Chr6:12650474-12650493 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 12648576 | 12650552 | 12648576 | ID=MsG0680030994.01;Name=MsG0680030994.01 |
| Chr6 | mRNA | 12648576 | 12650552 | 12648576 | ID=MsG0680030994.01.T01;Parent=MsG0680030994.01;Name=MsG0680030994.01.T01;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|2|0|359 |
| Chr6 | exon | 12649865 | 12650552 | 12649865 | ID=MsG0680030994.01.T01:exon:8962;Parent=MsG0680030994.01.T01 |
| Chr6 | exon | 12648576 | 12648967 | 12648576 | ID=MsG0680030994.01.T01:exon:8961;Parent=MsG0680030994.01.T01 |
| Chr6 | CDS | 12649865 | 12650552 | 12649865 | ID=MsG0680030994.01.T01:cds;Parent=MsG0680030994.01.T01 |
| Chr6 | CDS | 12648576 | 12648967 | 12648576 | ID=MsG0680030994.01.T01:cds;Parent=MsG0680030994.01.T01 |
| Gene Sequence |
| Protein sequence |
