AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040559.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040559.01.T01 MTR_7g092720 100.000 122 0 0 1 122 1 122 1.16e-87 250
MsG0780040559.01.T01 MTR_0157s0070 98.361 122 2 0 1 122 1 122 7.39e-87 248
MsG0780040559.01.T01 MTR_4g034030 98.361 122 2 0 1 122 1 122 7.39e-87 248
MsG0780040559.01.T01 MTR_2g085090 97.500 120 3 0 3 122 4 123 1.02e-83 240
MsG0780040559.01.T01 MTR_1g091057 97.500 120 3 0 3 122 4 123 1.02e-83 240
MsG0780040559.01.T01 MTR_4g034030 98.824 85 1 0 38 122 3 87 3.14e-58 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040559.01.T01 AT3G47370 89.344 122 13 0 1 122 1 122 2.32e-76 222
MsG0780040559.01.T01 AT3G47370 89.344 122 13 0 1 122 1 122 2.32e-76 222
MsG0780040559.01.T01 AT3G47370 89.344 122 13 0 1 122 1 122 2.32e-76 222
MsG0780040559.01.T01 AT5G62300 89.256 121 13 0 2 122 4 124 2.00e-75 219
MsG0780040559.01.T01 AT5G62300 89.256 121 13 0 2 122 4 124 2.00e-75 219
MsG0780040559.01.T01 AT3G45030 89.256 121 13 0 2 122 4 124 2.00e-75 219

Find 50 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTGAGTGAATAGGTATT+AGG 0.271593 7:-79190694 None:intergenic
CACTGAGTGAATAGGTATTA+GGG 0.329624 7:-79190693 None:intergenic
GTTATTAGTTTGTGCTGATT+TGG 0.351954 7:+79190929 MsG0780040559.01.T01:intron
AAAGGGTGATCCTAATCTTA+TGG 0.356442 7:-79190796 None:intergenic
TTCTCCACAAGGAGTCTTCC+TGG 0.359877 7:-79191027 None:intergenic
TAAACTCTGAACAATCAAAA+TGG 0.366742 7:+79190718 MsG0780040559.01.T01:exon
GTAATCTGTTTAACAACATC+TGG 0.381438 7:-79192045 None:intergenic
AATCTTATGGATCTGCTCTT+GGG 0.403761 7:-79190783 None:intergenic
TAATCTTATGGATCTGCTCT+TGG 0.405883 7:-79190784 None:intergenic
GGATTCCTCCAAACCAGGCT+TGG 0.415376 7:-79190762 None:intergenic
GAAACCCACCAAGCCTGGTT+TGG 0.420702 7:+79190754 MsG0780040559.01.T01:CDS
TCCTCCAAACCAGGCTTGGT+GGG 0.429749 7:-79190758 None:intergenic
TTCCTCCAAACCAGGCTTGG+TGG 0.433644 7:-79190759 None:intergenic
GTGATATGAAGAACCTTAGT+AGG 0.436083 7:-79191002 None:intergenic
TTAGAGTGAAGGGTCCTGTT+AGG 0.436691 7:+79190976 MsG0780040559.01.T01:CDS
ACCTTAGTAGGCATCCTAAC+AGG 0.444549 7:-79190990 None:intergenic
AAGAGCAGATCCATAAGATT+AGG 0.460004 7:+79190786 MsG0780040559.01.T01:CDS
AGTTTAAACACTGAGTGAAT+AGG 0.463482 7:-79190701 None:intergenic
TTGTTGCAGGTACCAACACC+TGG 0.473020 7:+79191971 MsG0780040559.01.T01:intron
TTTGACATGCTTGGAGGAAA+GGG 0.476236 7:-79190813 None:intergenic
AGGTATTAGGGCGCCGCAGC+TGG 0.488453 7:-79190681 None:intergenic
ACCAGGTTCGATGGTGATAG+AGG 0.489959 7:-79192067 None:intergenic
GTTTGTGCTGATTTGGTTCG+TGG 0.496204 7:+79190936 MsG0780040559.01.T01:intron
GCGATGAAACCCACCAAGCC+TGG 0.496657 7:+79190749 MsG0780040559.01.T01:CDS
CGAACCTGGTGTCGAGGTTG+AGG 0.501443 7:+79192080 MsG0780040559.01.T01:CDS
TTTGTTATTTGGGACAAAGT+TGG 0.502217 7:+79192187 MsG0780040559.01.T01:three_prime_UTR
AGGAAGACTCCTTGTGGAGA+AGG 0.508893 7:+79191029 MsG0780040559.01.T01:CDS
TCCTGTTAGGATGCCTACTA+AGG 0.508963 7:+79190989 MsG0780040559.01.T01:CDS
ACCCACCAAGCCTGGTTTGG+AGG 0.512859 7:+79190757 MsG0780040559.01.T01:CDS
TCTTGGGATTCCTCCAAACC+AGG 0.514536 7:-79190767 None:intergenic
ACCTCTATCACCATCGAACC+TGG 0.519945 7:+79192066 MsG0780040559.01.T01:CDS
TAATCTGTTTAACAACATCT+GGG 0.529679 7:-79192044 None:intergenic
TGATTTGGTTCGTGGCGCTA+AGG 0.536928 7:+79190944 MsG0780040559.01.T01:CDS
AACCTCGACACCAGGTTCGA+TGG 0.552766 7:-79192076 None:intergenic
CACCATCGAACCTGGTGTCG+AGG 0.559437 7:+79192074 MsG0780040559.01.T01:CDS
CTCCTTTGATTCACCAGCTG+CGG 0.563398 7:+79190668 None:intergenic
CGATAACTCTCTTGTGCACA+CGG 0.563868 7:-79192008 None:intergenic
GGATAAGCATCTTAGAGTGA+AGG 0.564807 7:+79190965 MsG0780040559.01.T01:CDS
TTCAAATCTATCCCAGGTGT+TGG 0.568966 7:-79191983 None:intergenic
GATAAGCATCTTAGAGTGAA+GGG 0.578400 7:+79190966 MsG0780040559.01.T01:CDS
GTAACCTCAACCTCGACACC+AGG 0.583580 7:-79192084 None:intergenic
CAACATCTGGGGAACTGTAG+AGG 0.590461 7:-79192032 None:intergenic
ATCAAGCATAAAGTTTCTCA+AGG 0.592726 7:-79192250 None:intergenic
ACGGAGTTCAAATCTATCCC+AGG 0.607565 7:-79191989 None:intergenic
AGGTTCTTCATATCACTACC+AGG 0.631821 7:+79191009 MsG0780040559.01.T01:CDS
TTAAGATCAAGCATCTGCAA+TGG 0.639875 7:-79192106 None:intergenic
AATGAAATACCTTCTCCACA+AGG 0.646547 7:-79191038 None:intergenic
ACTACCAGGAAGACTCCTTG+TGG 0.667890 7:+79191023 MsG0780040559.01.T01:CDS
TGTTGCAGGTACCAACACCT+GGG 0.671114 7:+79191972 MsG0780040559.01.T01:intron
AATCTGTTTAACAACATCTG+GGG 0.693040 7:-79192043 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATAGTTTTGTTATT+TGG + Chr7:79192176-79192195 MsG0780040559.01.T01:three_prime_UTR 10.0%
!!! TTCTCTTTAAAATAATAAAT+GGG + Chr7:79191407-79191426 MsG0780040559.01.T01:intron 10.0%
!!! TTTTTATAGTTTTGTTATTT+GGG + Chr7:79192177-79192196 MsG0780040559.01.T01:three_prime_UTR 10.0%
!!! GTTCTCTTTAAAATAATAAA+TGG + Chr7:79191406-79191425 MsG0780040559.01.T01:intron 15.0%
!!! TTTTATGATTATTTGATGAA+AGG + Chr7:79191104-79191123 MsG0780040559.01.T01:intron 15.0%
!! ACAAAACTATAAAAATACAG+AGG - Chr7:79192172-79192191 None:intergenic 20.0%
!! AGACATAACAAGTTAAAAAT+GGG - Chr7:79192133-79192152 None:intergenic 20.0%
!! ATAATAACAAAATGCAAAAG+AGG - Chr7:79191781-79191800 None:intergenic 20.0%
!! CAAAACTATAAAAATACAGA+GGG - Chr7:79192171-79192190 None:intergenic 20.0%
!!! TTTAATTCAGTTTTGTTGAA+TGG + Chr7:79191608-79191627 MsG0780040559.01.T01:intron 20.0%
! AATTATCCTACAACCTTAAT+TGG + Chr7:79191510-79191529 MsG0780040559.01.T01:intron 25.0%
! CAGACATAACAAGTTAAAAA+TGG - Chr7:79192134-79192153 None:intergenic 25.0%
! TAAACTCTGAACAATCAAAA+TGG + Chr7:79190718-79190737 MsG0780040559.01.T01:exon 25.0%
! TAATCTGTTTAACAACATCT+GGG - Chr7:79192047-79192066 None:intergenic 25.0%
!! TGAGAGCATAAAAAACATAA+AGG - Chr7:79190871-79190890 None:intergenic 25.0%
!!! GCATTTTGTTATTATGCTAT+TGG + Chr7:79191786-79191805 MsG0780040559.01.T01:intron 25.0%
!!! TGTTTCTGAACAAATTTTGA+AGG + Chr7:79191653-79191672 MsG0780040559.01.T01:intron 25.0%
AATCTGTTTAACAACATCTG+GGG - Chr7:79192046-79192065 None:intergenic 30.0%
AGATTAGAAACATCATTGAG+AGG + Chr7:79191142-79191161 MsG0780040559.01.T01:intron 30.0%
AGTTTAAACACTGAGTGAAT+AGG - Chr7:79190704-79190723 None:intergenic 30.0%
ATCAAGCATAAAGTTTCTCA+AGG - Chr7:79192253-79192272 None:intergenic 30.0%
ATTTGATGAAAGGTAGTCAA+TGG + Chr7:79191114-79191133 MsG0780040559.01.T01:intron 30.0%
GATTAGAAACATCATTGAGA+GGG + Chr7:79191143-79191162 MsG0780040559.01.T01:intron 30.0%
GTAATCTGTTTAACAACATC+TGG - Chr7:79192048-79192067 None:intergenic 30.0%
TAAAGAGAACAAACCAAACA+CGG - Chr7:79191396-79191415 None:intergenic 30.0%
TCCTAATATGGCTTACAAAT+GGG + Chr7:79191283-79191302 MsG0780040559.01.T01:intron 30.0%
! GTTTTTCTAAGCTCCTAATA+TGG + Chr7:79191271-79191290 MsG0780040559.01.T01:intron 30.0%
!! GTTATTAGTTTGTGCTGATT+TGG + Chr7:79190929-79190948 MsG0780040559.01.T01:intron 30.0%
!! TTTGTTATTTGGGACAAAGT+TGG + Chr7:79192187-79192206 MsG0780040559.01.T01:three_prime_UTR 30.0%
!!! GGCTTGTTTTCTTTAGTTTT+CGG + Chr7:79192208-79192227 MsG0780040559.01.T01:three_prime_UTR 30.0%
!!! TTTTCTTTAGTTTTCGGTTC+TGG + Chr7:79192214-79192233 MsG0780040559.01.T01:three_prime_UTR 30.0%
AAAGGGTGATCCTAATCTTA+TGG - Chr7:79190799-79190818 None:intergenic 35.0%
AAATACGATATAGCCGTGTT+TGG + Chr7:79191380-79191399 MsG0780040559.01.T01:intron 35.0%
AAGAGCAGATCCATAAGATT+AGG + Chr7:79190786-79190805 MsG0780040559.01.T01:CDS 35.0%
AATCTTATGGATCTGCTCTT+GGG - Chr7:79190786-79190805 None:intergenic 35.0%
AATGAAATACCTTCTCCACA+AGG - Chr7:79191041-79191060 None:intergenic 35.0%
ACACTGAGTGAATAGGTATT+AGG - Chr7:79190697-79190716 None:intergenic 35.0%
ACCCATTTGTAAGCCATATT+AGG - Chr7:79191287-79191306 None:intergenic 35.0%
ATCCTACACTTCAATGACAA+TGG - Chr7:79191893-79191912 None:intergenic 35.0%
CACTGAGTGAATAGGTATTA+GGG - Chr7:79190696-79190715 None:intergenic 35.0%
CATGTCAAAAATCTCGAGAA+GGG + Chr7:79190827-79190846 MsG0780040559.01.T01:CDS 35.0%
CTCCTAATATGGCTTACAAA+TGG + Chr7:79191282-79191301 MsG0780040559.01.T01:intron 35.0%
GATAAGCATCTTAGAGTGAA+GGG + Chr7:79190966-79190985 MsG0780040559.01.T01:CDS 35.0%
GCATCTAAATTTCCATAAGC+AGG - Chr7:79191336-79191355 None:intergenic 35.0%
GTGATATGAAGAACCTTAGT+AGG - Chr7:79191005-79191024 None:intergenic 35.0%
TAATCTTATGGATCTGCTCT+TGG - Chr7:79190787-79190806 None:intergenic 35.0%
TGTGCATAGAAGAGAATCAA+CGG - Chr7:79191744-79191763 None:intergenic 35.0%
TTAAGATCAAGCATCTGCAA+TGG - Chr7:79192109-79192128 None:intergenic 35.0%
!! AATTTTGAAGGACTCGACTA+CGG + Chr7:79191665-79191684 MsG0780040559.01.T01:intron 35.0%
!!! CTTGATAGTGCTATGTTTTC+TGG + Chr7:79191850-79191869 MsG0780040559.01.T01:intron 35.0%
AAACCAATAGACGCAGAAGA+AGG - Chr7:79191447-79191466 None:intergenic 40.0%
AACCAATAGACGCAGAAGAA+GGG - Chr7:79191446-79191465 None:intergenic 40.0%
AGCCATTGTCATTGAAGTGT+AGG + Chr7:79191888-79191907 MsG0780040559.01.T01:intron 40.0%
AGGTTCTTCATATCACTACC+AGG + Chr7:79191009-79191028 MsG0780040559.01.T01:CDS 40.0%
ATGACCTTACTTCTTGTTGC+AGG + Chr7:79191958-79191977 MsG0780040559.01.T01:intron 40.0%
CACTATCAAGTATTCAGCAG+AGG - Chr7:79191840-79191859 None:intergenic 40.0%
CGTATGCCAATTAAGGTTGT+AGG - Chr7:79191519-79191538 None:intergenic 40.0%
GCATGTCAAAAATCTCGAGA+AGG + Chr7:79190826-79190845 MsG0780040559.01.T01:CDS 40.0%
GGATAAGCATCTTAGAGTGA+AGG + Chr7:79190965-79190984 MsG0780040559.01.T01:CDS 40.0%
TACGTGTGAGAGTAAAAGGA+AGG + Chr7:79191535-79191554 MsG0780040559.01.T01:intron 40.0%
TCAAGTATTCAGCAGAGGTT+TGG - Chr7:79191835-79191854 None:intergenic 40.0%
TCTCACACGTATGCCAATTA+AGG - Chr7:79191526-79191545 None:intergenic 40.0%
TGAGAGGGAACTGTTGAAAT+AGG + Chr7:79191158-79191177 MsG0780040559.01.T01:intron 40.0%
TGATTCTCTTCTATGCACAC+TGG + Chr7:79191745-79191764 MsG0780040559.01.T01:intron 40.0%
TTCAAATCTATCCCAGGTGT+TGG - Chr7:79191986-79192005 None:intergenic 40.0%
TTTGACATGCTTGGAGGAAA+GGG - Chr7:79190816-79190835 None:intergenic 40.0%
! CTCGAGATTTTTGACATGCT+TGG - Chr7:79190825-79190844 None:intergenic 40.0%
! GAGATTTTTGACATGCTTGG+AGG - Chr7:79190822-79190841 None:intergenic 40.0%
! TTTTGACATGCTTGGAGGAA+AGG - Chr7:79190817-79190836 None:intergenic 40.0%
!! ATTTTGAAGGACTCGACTAC+GGG + Chr7:79191666-79191685 MsG0780040559.01.T01:intron 40.0%
ACCAATAGACGCAGAAGAAG+GGG - Chr7:79191445-79191464 None:intergenic 45.0%
ACCTTAGTAGGCATCCTAAC+AGG - Chr7:79190993-79191012 None:intergenic 45.0%
ACGGAGTTCAAATCTATCCC+AGG - Chr7:79191992-79192011 None:intergenic 45.0%
CGATAACTCTCTTGTGCACA+CGG - Chr7:79192011-79192030 None:intergenic 45.0%
GGCATACGTGTGAGAGTAAA+AGG + Chr7:79191531-79191550 MsG0780040559.01.T01:intron 45.0%
GGTACCTGCAACAAGAAGTA+AGG - Chr7:79191965-79191984 None:intergenic 45.0%
TCCTGTTAGGATGCCTACTA+AGG + Chr7:79190989-79191008 MsG0780040559.01.T01:CDS 45.0%
TTAGAGTGAAGGGTCCTGTT+AGG + Chr7:79190976-79190995 MsG0780040559.01.T01:CDS 45.0%
! TTCTATGCACACTGGTTGCA+AGG + Chr7:79191753-79191772 MsG0780040559.01.T01:intron 45.0%
!! CCATAAGCAGGCTAGTTTCA+GGG - Chr7:79191324-79191343 None:intergenic 45.0%
!! CGACTACGGGTTGGAATTAA+TGG + Chr7:79191679-79191698 MsG0780040559.01.T01:intron 45.0%
!! GTTTGTGCTGATTTGGTTCG+TGG + Chr7:79190936-79190955 MsG0780040559.01.T01:intron 45.0%
!! TCCATAAGCAGGCTAGTTTC+AGG - Chr7:79191325-79191344 None:intergenic 45.0%
ACCAGGTTCGATGGTGATAG+AGG - Chr7:79192070-79192089 None:intergenic 50.0%
ACCTCTATCACCATCGAACC+TGG + Chr7:79192066-79192085 MsG0780040559.01.T01:CDS 50.0%
ACTACCAGGAAGACTCCTTG+TGG + Chr7:79191023-79191042 MsG0780040559.01.T01:CDS 50.0%
AGGAAGACTCCTTGTGGAGA+AGG + Chr7:79191029-79191048 MsG0780040559.01.T01:CDS 50.0%
CAACATCTGGGGAACTGTAG+AGG - Chr7:79192035-79192054 None:intergenic 50.0%
TCCCCTTCTTCTGCGTCTAT+TGG + Chr7:79191441-79191460 MsG0780040559.01.T01:intron 50.0%
TCTTGGGATTCCTCCAAACC+AGG - Chr7:79190770-79190789 None:intergenic 50.0%
TGTTGCAGGTACCAACACCT+GGG + Chr7:79191972-79191991 MsG0780040559.01.T01:intron 50.0%
TTCTCCACAAGGAGTCTTCC+TGG - Chr7:79191030-79191049 None:intergenic 50.0%
TTGTTGCAGGTACCAACACC+TGG + Chr7:79191971-79191990 MsG0780040559.01.T01:intron 50.0%
! CCCTGAAACTAGCCTGCTTA+TGG + Chr7:79191321-79191340 MsG0780040559.01.T01:intron 50.0%
!! CTGCGTCTATTGGTTTGTGC+TGG + Chr7:79191451-79191470 MsG0780040559.01.T01:intron 50.0%
!! TGATTTGGTTCGTGGCGCTA+AGG + Chr7:79190944-79190963 MsG0780040559.01.T01:CDS 50.0%
AACCTCGACACCAGGTTCGA+TGG - Chr7:79192079-79192098 None:intergenic 55.0%
GAAACCCACCAAGCCTGGTT+TGG + Chr7:79190754-79190773 MsG0780040559.01.T01:CDS 55.0%
GGATTCCTCCAAACCAGGCT+TGG - Chr7:79190765-79190784 None:intergenic 55.0%
GTAACCTCAACCTCGACACC+AGG - Chr7:79192087-79192106 None:intergenic 55.0%
TGAAGGACTCGACTACGGGT+TGG + Chr7:79191670-79191689 MsG0780040559.01.T01:intron 55.0%
TTCCTCCAAACCAGGCTTGG+TGG - Chr7:79190762-79190781 None:intergenic 55.0%
! TCCTCCAAACCAGGCTTGGT+GGG - Chr7:79190761-79190780 None:intergenic 55.0%
GCGATGAAACCCACCAAGCC+TGG + Chr7:79190749-79190768 MsG0780040559.01.T01:CDS 60.0%
! CACCATCGAACCTGGTGTCG+AGG + Chr7:79192074-79192093 MsG0780040559.01.T01:CDS 60.0%
! CGAACCTGGTGTCGAGGTTG+AGG + Chr7:79192080-79192099 MsG0780040559.01.T01:CDS 60.0%
!! ACCCACCAAGCCTGGTTTGG+AGG + Chr7:79190757-79190776 MsG0780040559.01.T01:CDS 60.0%
!! AGGTATTAGGGCGCCGCAGC+TGG - Chr7:79190684-79190703 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 79190675 79192333 79190675 ID=MsG0780040559.01;Name=MsG0780040559.01
Chr7 mRNA 79190675 79192333 79190675 ID=MsG0780040559.01.T01;Parent=MsG0780040559.01;Name=MsG0780040559.01.T01;_AED=0.32;_eAED=0.32;_QI=62|1|1|1|1|1|3|211|122
Chr7 exon 79190675 79190848 79190675 ID=MsG0780040559.01.T01:exon:473;Parent=MsG0780040559.01.T01
Chr7 exon 79190937 79191050 79190937 ID=MsG0780040559.01.T01:exon:474;Parent=MsG0780040559.01.T01
Chr7 exon 79191980 79192333 79191980 ID=MsG0780040559.01.T01:exon:475;Parent=MsG0780040559.01.T01
Chr7 five_prime_UTR 79190675 79190736 79190675 ID=MsG0780040559.01.T01:five_prime_utr;Parent=MsG0780040559.01.T01
Chr7 CDS 79190737 79190848 79190737 ID=MsG0780040559.01.T01:cds;Parent=MsG0780040559.01.T01
Chr7 CDS 79190937 79191050 79190937 ID=MsG0780040559.01.T01:cds;Parent=MsG0780040559.01.T01
Chr7 CDS 79191980 79192122 79191980 ID=MsG0780040559.01.T01:cds;Parent=MsG0780040559.01.T01
Chr7 three_prime_UTR 79192123 79192333 79192123 ID=MsG0780040559.01.T01:three_prime_utr;Parent=MsG0780040559.01.T01
Gene Sequence

>MsG0780040559.01.T01

ATGGCGTACGCTGCGATGAAACCCACCAAGCCTGGTTTGGAGGAATCCCAAGAGCAGATCCATAAGATTAGGATCACCCTTTCCTCCAAGCATGTCAAAAATCTCGAGAAGGTTTGTGCTGATTTGGTTCGTGGCGCTAAGGATAAGCATCTTAGAGTGAAGGGTCCTGTTAGGATGCCTACTAAGGTTCTTCATATCACTACCAGGAAGACTCCTTGTGGAGAAGGTACCAACACCTGGGATAGATTTGAACTCCGTGTGCACAAGAGAGTTATCGACCTCTACAGTTCCCCAGATGTTGTTAAACAGATTACCTCTATCACCATCGAACCTGGTGTCGAGGTTGAGGTTACCATTGCAGATGCTTGA

Protein sequence

>MsG0780040559.01.T01

MAYAAMKPTKPGLEESQEQIHKIRITLSSKHVKNLEKVCADLVRGAKDKHLRVKGPVRMPTKVLHITTRKTPCGEGTNTWDRFELRVHKRVIDLYSSPDVVKQITSITIEPGVEVEVTIADA*