AlfalfaGEDB | MsG0880047306.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047306.01

KEGG: K00921
GO: GO:0016307 GO:0046488
PFAM: PF01504

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047306.01.T02	XP_039688841.1	79.592	245	18	2	2	217	1145	1386	1.19E-105	353
MsG0880047306.01.T02	XP_039688841.1	71.023	176	48	2	221	394	1503	1677	4.57E-65	237
MsG0880047306.01.T01	XP_039688841.1	79.592	245	18	2	2	217	1145	1386	1.19E-105	353
MsG0880047306.01.T01	XP_039688841.1	71.023	176	48	2	221	394	1503	1677	4.57E-65	237

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047306.01.T01	Q9LUM0	60.227	176	67	2	221	394	1483	1657	1.05E-56	204
MsG0880047306.01.T02	Q9LUM0	60.227	176	67	2	221	394	1483	1657	1.05E-56	204

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047306.01.T01	A0A396INN2	79.592	245	18	2	2	217	1144	1385	6.02e-106	353
MsG0880047306.01.T02	A0A396INN2	79.592	245	18	2	2	217	1144	1385	6.02e-106	353

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048463.01	MsG0880047306.01	0.805464	1.318050e-49	6.978059e-47
MsG0080048464.01	MsG0880047306.01	0.812415	4.288369e-51	2.725111e-48
MsG0080048938.01	MsG0880047306.01	0.826123	3.224357e-54	2.996817e-51
MsG0180000159.01	MsG0880047306.01	0.835249	1.872870e-56	2.277502e-53
MsG0180000246.01	MsG0880047306.01	0.816558	5.198291e-52	3.695462e-49
MsG0180000599.01	MsG0880047306.01	0.803485	3.408007e-49	1.714713e-46
MsG0180000646.01	MsG0880047306.01	0.836063	1.165547e-56	1.452352e-53
MsG0180000647.01	MsG0880047306.01	0.843582	1.282605e-58	2.016014e-55
MsG0180000814.01	MsG0880047306.01	0.802303	5.981616e-49	2.920184e-46
MsG0180000843.01	MsG0880047306.01	0.802773	4.785343e-49	2.364266e-46
MsG0180001100.01	MsG0880047306.01	0.834793	2.440624e-56	2.926915e-53
MsG0180001105.01	MsG0880047306.01	0.807306	5.392107e-50	2.993082e-47
MsG0180001119.01	MsG0880047306.01	0.804645	1.955660e-49	1.013740e-46
MsG0180002024.01	MsG0880047306.01	0.821160	4.680870e-53	3.778814e-50
MsG0880044043.01	MsG0880047306.01	0.833370	5.545779e-56	6.373930e-53
MsG0880045285.01	MsG0880047306.01	0.822354	2.478172e-53	2.069316e-50
MsG0880045323.01	MsG0880047306.01	0.830724	2.500123e-55	2.656358e-52
MsG0880045771.01	MsG0880047306.01	0.838262	3.192775e-57	4.253009e-54
MsG0880045783.01	MsG0880047306.01	0.812551	4.004650e-51	2.553896e-48
MsG0880045992.01	MsG0880047306.01	0.818014	2.444692e-52	1.808668e-49
MsG0880046268.01	MsG0880047306.01	0.814055	1.871912e-51	1.243033e-48
MsG0880046296.01	MsG0880047306.01	0.814787	1.289623e-51	8.734970e-49
MsG0880046723.01	MsG0880047306.01	0.839668	1.381075e-57	1.920628e-54
MsG0880047046.01	MsG0880047306.01	0.807844	4.144850e-50	2.333096e-47
MsG0880047306.01	MsG0880047394.01	0.851491	8.574023e-61	1.738374e-57
MsG0880047306.01	MsG0880047432.01	0.825316	5.008810e-54	4.549766e-51
MsG0880047306.01	MsG0880047770.01	0.805961	1.036887e-49	5.559946e-47
MsG0180003991.01	MsG0880047306.01	-0.810969	8.846978e-51	5.409511e-48
MsG0180004103.01	MsG0880047306.01	0.810018	1.420102e-50	8.466987e-48
MsG0180004112.01	MsG0880047306.01	0.819526	1.108878e-52	8.554421e-50
MsG0180004246.01	MsG0880047306.01	0.823217	1.560116e-53	1.334916e-50
MsG0180005292.01	MsG0880047306.01	0.828348	9.453509e-55	9.371413e-52
MsG0180005418.01	MsG0880047306.01	0.814173	1.762610e-51	1.174211e-48
MsG0180005639.01	MsG0880047306.01	0.800690	1.280135e-48	5.998859e-46
MsG0180005829.01	MsG0880047306.01	0.845446	4.040318e-59	6.734713e-56
MsG0180006054.01	MsG0880047306.01	0.844268	8.397891e-59	1.348551e-55
MsG0280006309.01	MsG0880047306.01	0.812860	3.427970e-51	2.203761e-48
MsG0780040285.01	MsG0880047306.01	0.800230	1.588581e-48	7.357895e-46
MsG0780040763.01	MsG0880047306.01	0.800058	1.722030e-48	7.941280e-46
MsG0780041088.01	MsG0880047306.01	0.821340	4.253988e-53	3.451406e-50
MsG0780041091.01	MsG0880047306.01	0.847278	1.279301e-59	2.262132e-56
MsG0780041187.01	MsG0880047306.01	0.863684	2.088285e-64	6.385866e-61
MsG0780041191.01	MsG0880047306.01	0.856234	3.691826e-62	8.751186e-59
MsG0780041262.01	MsG0880047306.01	0.803860	2.848956e-49	1.447329e-46
MsG0780041301.01	MsG0880047306.01	0.857736	1.332493e-62	3.321742e-59
MsG0780041468.01	MsG0880047306.01	0.821100	4.830212e-53	3.893070e-50
MsG0880041852.01	MsG0880047306.01	0.823220	1.557134e-53	1.332518e-50
MsG0880042000.01	MsG0880047306.01	0.812470	4.171575e-51	2.654786e-48
MsG0880042022.01	MsG0880047306.01	0.818843	1.586494e-52	1.200956e-49
MsG0880043157.01	MsG0880047306.01	0.822990	1.762606e-53	1.498618e-50
MsG0280010807.01	MsG0880047306.01	0.801526	8.635825e-49	4.133365e-46
MsG0280011272.01	MsG0880047306.01	-0.800712	1.267052e-48	5.940624e-46
MsG0280011401.01	MsG0880047306.01	0.817092	3.944968e-52	2.845814e-49
MsG0280011404.01	MsG0880047306.01	0.848668	5.292960e-60	9.786206e-57
MsG0380012567.01	MsG0880047306.01	0.802243	6.154775e-49	2.999956e-46
MsG0380013342.01	MsG0880047306.01	0.850508	1.622484e-60	3.185098e-57
MsG0380014517.01	MsG0880047306.01	0.809340	1.985578e-50	1.162792e-47
MsG0680031998.01	MsG0880047306.01	0.810727	9.981860e-51	6.063707e-48
MsG0680032254.01	MsG0880047306.01	0.808572	2.899589e-50	1.663702e-47
MsG0680032827.01	MsG0880047306.01	0.822947	1.803585e-53	1.531567e-50
MsG0680034721.01	MsG0880047306.01	0.826385	2.793413e-54	2.615850e-51
MsG0680035307.01	MsG0880047306.01	0.816924	4.301961e-52	3.089239e-49
MsG0480019065.01	MsG0880047306.01	0.801514	8.686659e-49	4.156368e-46
MsG0480019711.01	MsG0880047306.01	0.804789	1.824849e-49	9.494922e-47
MsG0480020322.01	MsG0880047306.01	0.818602	1.799489e-52	1.353044e-49
MsG0480020534.01	MsG0880047306.01	-0.802943	4.413613e-49	2.189904e-46
MsG0480020935.01	MsG0880047306.01	-0.812097	5.033116e-51	3.170761e-48
MsG0480021207.01	MsG0880047306.01	0.808632	2.815626e-50	1.617977e-47
MsG0480021564.01	MsG0880047306.01	0.821794	3.339995e-53	2.744768e-50
MsG0480022178.01	MsG0880047306.01	0.831346	1.758456e-55	1.903023e-52
MsG0480022617.01	MsG0880047306.01	0.855135	7.730324e-62	1.766649e-58
MsG0480022624.01	MsG0880047306.01	0.841584	4.348324e-58	6.418554e-55
MsG0480022802.01	MsG0880047306.01	0.819663	1.031696e-52	7.989151e-50
MsG0480022918.01	MsG0880047306.01	0.858479	8.010549e-63	2.047390e-59
MsG0480023045.01	MsG0880047306.01	0.803333	3.665680e-49	1.837073e-46
MsG0480023381.01	MsG0880047306.01	0.833316	5.721383e-56	6.565157e-53
MsG0480023877.01	MsG0880047306.01	0.866000	3.924790e-65	1.302619e-61
MsG0580024717.01	MsG0880047306.01	0.859248	4.715731e-63	1.237234e-59
MsG0580025178.01	MsG0880047306.01	0.815184	1.052642e-51	7.206693e-49
MsG0580025827.01	MsG0880047306.01	0.858250	9.370088e-63	2.376288e-59
MsG0580025832.01	MsG0880047306.01	0.829511	4.941041e-55	5.067687e-52
MsG0380015847.01	MsG0880047306.01	-0.814482	1.506488e-51	1.012075e-48
MsG0380016187.01	MsG0880047306.01	0.806998	6.263730e-50	3.449081e-47
MsG0380016527.01	MsG0880047306.01	0.829448	5.118519e-55	5.240108e-52
MsG0380016762.01	MsG0880047306.01	0.819491	1.129608e-52	8.705663e-50
MsG0380016879.01	MsG0880047306.01	0.816908	4.338857e-52	3.114244e-49
MsG0380017202.01	MsG0880047306.01	0.815296	9.943823e-52	6.828941e-49
MsG0380017293.01	MsG0880047306.01	0.866112	3.616918e-65	1.205080e-61
MsG0380017661.01	MsG0880047306.01	0.842788	2.087370e-58	3.199478e-55
MsG0380017931.01	MsG0880047306.01	0.844762	6.185698e-59	1.008891e-55
MsG0480018226.01	MsG0880047306.01	0.801921	7.165612e-49	3.464135e-46
MsG0480018236.01	MsG0880047306.01	0.816900	4.357715e-52	3.127023e-49
MsG0580026304.01	MsG0880047306.01	0.827290	1.697978e-54	1.632243e-51
MsG0580026510.01	MsG0880047306.01	0.809380	1.947242e-50	1.141550e-47
MsG0580027849.01	MsG0880047306.01	0.809319	2.006859e-50	1.174548e-47
MsG0580028024.01	MsG0880047306.01	0.806857	6.710449e-50	3.681707e-47
MsG0580028925.01	MsG0880047306.01	0.819034	1.435676e-52	1.092545e-49
MsG0580029038.01	MsG0880047306.01	0.819736	9.928168e-53	7.703909e-50
MsG0580029414.01	MsG0880047306.01	0.827033	1.956090e-54	1.866153e-51
MsG0680030464.01	MsG0880047306.01	0.804775	1.836958e-49	9.554458e-47
MsG0680030514.01	MsG0880047306.01	0.824669	7.122881e-54	6.352155e-51
MsG0680030566.01	MsG0880047306.01	-0.801209	1.003243e-48	4.763462e-46
MsG0680030680.01	MsG0880047306.01	0.803515	3.360556e-49	1.692147e-46
MsG0280006770.01	MsG0880047306.01	0.802201	6.277193e-49	3.056226e-46
MsG0280006896.01	MsG0880047306.01	0.804819	1.798397e-49	9.365034e-47
MsG0280007118.01	MsG0880047306.01	0.810837	9.449858e-51	5.757477e-48
MsG0280007877.01	MsG0880047306.01	0.802358	5.825802e-49	2.848119e-46
MsG0280008103.01	MsG0880047306.01	0.823828	1.122260e-53	9.770767e-51
MsG0280008112.01	MsG0880047306.01	0.810691	1.016316e-50	6.167780e-48
MsG0280008717.01	MsG0880047306.01	0.804481	2.115693e-49	1.092049e-46
MsG0280009256.01	MsG0880047306.01	0.805684	1.185240e-49	6.310662e-47
MsG0280009507.01	MsG0880047306.01	0.812183	4.818184e-51	3.042528e-48
MsG0280009757.01	MsG0880047306.01	0.805350	1.392783e-49	7.351889e-47
MsG0680035707.01	MsG0880047306.01	0.868170	7.965631e-66	2.854040e-62
MsG0780036517.01	MsG0880047306.01	0.843654	1.227152e-58	1.933211e-55
MsG0780036567.01	MsG0880047306.01	0.824348	8.476334e-54	7.488149e-51
MsG0780036593.01	MsG0880047306.01	0.811467	6.900038e-51	4.274488e-48
MsG0780036596.01	MsG0880047306.01	0.814918	1.205956e-51	8.196968e-49
MsG0780036803.01	MsG0880047306.01	0.822171	2.732803e-53	2.269829e-50
MsG0780037679.01	MsG0880047306.01	0.809663	1.693118e-50	1.000079e-47
MsG0780037701.01	MsG0880047306.01	0.814794	1.284726e-51	8.703561e-49
MsG0780039256.01	MsG0880047306.01	0.813769	2.164584e-51	1.426255e-48
MsG0780039435.01	MsG0880047306.01	0.852492	4.454435e-61	9.323910e-58
MsG0780039975.01	MsG0880047306.01	0.829698	4.449945e-55	4.589578e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047306.01.T02	MTR_4g124590	79.592	245	18	2	2	217	1142	1383	1.74e-109	353
MsG0880047306.01.T02	MTR_4g124590	71.023	176	48	2	221	394	1500	1674	7.59e-69	236
MsG0880047306.01.T02	MTR_8g023080	60.795	176	66	2	221	394	1500	1674	2.42e-57	203
MsG0880047306.01.T02	MTR_8g023080	60.795	176	66	2	221	394	1508	1682	2.64e-57	203
MsG0880047306.01.T02	MTR_4g085090	50.000	174	84	2	223	393	1442	1615	4.28e-46	170
MsG0880047306.01.T02	MTR_1g097110	43.787	169	88	2	229	393	1298	1463	2.22e-34	136
MsG0880047306.01.T01	MTR_4g124590	79.592	245	18	2	2	217	1142	1383	1.74e-109	353
MsG0880047306.01.T01	MTR_4g124590	71.023	176	48	2	221	394	1500	1674	7.59e-69	236
MsG0880047306.01.T01	MTR_8g023080	60.795	176	66	2	221	394	1500	1674	2.42e-57	203
MsG0880047306.01.T01	MTR_8g023080	60.795	176	66	2	221	394	1508	1682	2.64e-57	203
MsG0880047306.01.T01	MTR_4g085090	50.000	174	84	2	223	393	1442	1615	4.28e-46	170
MsG0880047306.01.T01	MTR_1g097110	43.787	169	88	2	229	393	1298	1463	2.22e-34	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047306.01.T02	AT3G14270	60.227	176	67	2	221	394	1483	1657	1.07e-57	204
MsG0880047306.01.T02	AT3G14270	60.227	176	67	2	221	394	1483	1657	1.07e-57	204
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1440	1614	3.19e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1404	1578	3.30e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1441	1615	3.48e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1443	1617	3.48e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T02	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T02	AT1G71010	51.220	164	79	1	231	393	1374	1537	3.09e-46	171
MsG0880047306.01.T02	AT1G34260	39.429	175	101	3	223	393	930	1103	3.03e-28	117
MsG0880047306.01.T02	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118
MsG0880047306.01.T02	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118
MsG0880047306.01.T02	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118
MsG0880047306.01.T01	AT3G14270	60.227	176	67	2	221	394	1483	1657	1.07e-57	204
MsG0880047306.01.T01	AT3G14270	60.227	176	67	2	221	394	1483	1657	1.07e-57	204
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1440	1614	3.19e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1404	1578	3.30e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1441	1615	3.48e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1443	1617	3.48e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T01	AT4G33240	57.386	176	72	2	221	394	1444	1618	3.54e-55	197
MsG0880047306.01.T01	AT1G71010	51.220	164	79	1	231	393	1374	1537	3.09e-46	171
MsG0880047306.01.T01	AT1G34260	39.429	175	101	3	223	393	930	1103	3.03e-28	117
MsG0880047306.01.T01	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118
MsG0880047306.01.T01	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118
MsG0880047306.01.T01	AT1G34260	39.429	175	101	3	223	393	1170	1343	3.14e-28	118

Find 54 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCAGTGAGACTATATTTA+AGG	0.141338	8:-84391919	MsG0880047306.01.T02:three_prime_UTR
GAATTCGAGATCAGTTATTT+TGG	0.224671	8:-84391795	MsG0880047306.01.T02:three_prime_UTR
GTGAAGACCTCAGGCATTCT+TGG	0.311099	8:-84392503	MsG0880047306.01.T01:three_prime_UTR
TTCATCACAAAGGTCGGTTT+GGG	0.348819	8:+84392349	None:intergenic
TTTCATCACAAAGGTCGGTT+TGG	0.354997	8:+84392348	None:intergenic
CATTGAACATATCAATCAAA+TGG	0.395209	8:+84392309	None:intergenic
GATTCACTGGTTTGTAAGCT+TGG	0.395316	8:+84392371	None:intergenic
GAATTCTTGATGCCAGCTAA+TGG	0.397202	8:+84391812	None:intergenic
TGAACATTCCTAGTAATTGA+AGG	0.404067	8:+84392134	None:intergenic
AACAGTTGGAGAAGTATTCT+TGG	0.404328	8:+84392475	None:intergenic
GGGACAAGCACCTTGAAACT+TGG	0.411571	8:-84392526	MsG0880047306.01.T01:three_prime_UTR
ATGGACTACTCATTGCTTGT+TGG	0.419723	8:-84392610	MsG0880047306.01.T01:three_prime_UTR
AGGTCTTCACCCAAGTTTCA+AGG	0.441536	8:+84392516	None:intergenic
TTCTTTATTGTTACTTCCTA+CGG	0.443048	8:-84391998	MsG0880047306.01.T02:three_prime_UTR
TTCTGATGGTGCCAGATCAA+TGG	0.443915	8:-84392400	MsG0880047306.01.T01:three_prime_UTR
CTACTCATTGCTTGTTGGTG+TGG	0.449179	8:-84392605	MsG0880047306.01.T01:three_prime_UTR
GGTTCTGCAATTCCATTAGC+TGG	0.471332	8:-84391824	MsG0880047306.01.T02:three_prime_UTR
AGCCTTAAATATAGTCTCAC+TGG	0.483391	8:+84391917	None:intergenic
GAAACTTGGGTGAAGACCTC+AGG	0.483603	8:-84392512	MsG0880047306.01.T01:three_prime_UTR
ACAGTTGGAGAAGTATTCTT+GGG	0.488998	8:+84392476	None:intergenic
ATTGAACATATCAATCAAAT+GGG	0.494010	8:+84392310	None:intergenic
GGAATGTTCAAAGGGTATGC+TGG	0.494036	8:-84392121	MsG0880047306.01.T01:three_prime_UTR
AATTGCAGAACCATTGCATC+AGG	0.500543	8:+84391835	None:intergenic
GGAGAAGCATGAGCTAGTTT+AGG	0.510559	8:-84392578	MsG0880047306.01.T01:three_prime_UTR
AAGGTCGGTTTGGGATTCAC+TGG	0.515020	8:+84392358	None:intergenic
CTTGGAGGAGACCATTGATC+TGG	0.521391	8:+84392389	None:intergenic
AATTACTAGGAATGTTCAAA+GGG	0.522672	8:-84392129	MsG0880047306.01.T01:three_prime_UTR
TCACTGGTTTGTAAGCTTGG+AGG	0.522870	8:+84392374	None:intergenic
TACAAATCATAACTTTCTGA+AGG	0.524325	8:-84392038	MsG0880047306.01.T01:three_prime_UTR
AATGCAGCAGAAATATATTG+AGG	0.526845	8:+84392217	None:intergenic
CAATTACTAGGAATGTTCAA+AGG	0.530411	8:-84392130	MsG0880047306.01.T01:three_prime_UTR
CACCACAGCAGTACAAGAAA+CGG	0.533946	8:-84392448	MsG0880047306.01.T01:three_prime_UTR
ATTGCTTGTTGGTGTGGATG+AGG	0.540346	8:-84392599	MsG0880047306.01.T01:three_prime_UTR
AGCATGGTAATACAGTTTCA+CGG	0.546798	8:+84391940	None:intergenic
TAGGAAGTAACAATAAAGAA+AGG	0.546800	8:+84392001	None:intergenic
AAGACCTCAGGCATTCTTGG+TGG	0.553682	8:-84392500	MsG0880047306.01.T01:three_prime_UTR
AAAGGAACTTCCTGATGCAA+TGG	0.557363	8:-84391845	MsG0880047306.01.T02:three_prime_UTR
ATGCAGCAGAAATATATTGA+GGG	0.562927	8:+84392218	None:intergenic
GCATGGTAATACAGTTTCAC+GGG	0.565081	8:+84391941	None:intergenic
AACCGTTTCTTGTACTGCTG+TGG	0.571276	8:+84392446	None:intergenic
GGACAAGCACCTTGAAACTT+GGG	0.580114	8:-84392525	MsG0880047306.01.T01:three_prime_UTR
TGCTGTGGTGATATAACAGT+TGG	0.582674	8:+84392461	None:intergenic
TTGTATTATGTGATAAATGC+AGG	0.582815	8:-84392196	MsG0880047306.01.T01:intron
AGCAACATAATCACTTGAGA+TGG	0.583809	8:-84391886	MsG0880047306.01.T02:three_prime_UTR
TAGGTGCACCTTCAATTACT+AGG	0.590351	8:-84392142	MsG0880047306.01.T01:three_prime_UTR
AATATAGTCTCACTGGAGCA+TGG	0.599148	8:+84391924	None:intergenic
GTTGCAGTCAATATATGTGA+TGG	0.618456	8:-84392629	MsG0880047306.01.T01:intron
AGCAGTACAAGAAACGGTTC+AGG	0.624969	8:-84392442	MsG0880047306.01.T01:three_prime_UTR
TATGCTGGATCATGTCTGCA+AGG	0.630409	8:-84392106	MsG0880047306.01.T01:three_prime_UTR
GGGTCCACCAAGAATGCCTG+AGG	0.649792	8:+84392496	None:intergenic
TGATGAAAATTCATGAGGTG+TGG	0.652370	8:-84392333	MsG0880047306.01.T01:intron
GTCAATATAGGGACTTCCGT+AGG	0.653154	8:+84391982	None:intergenic
CTTTGTGATGAAAATTCATG+AGG	0.670017	8:-84392338	MsG0880047306.01.T01:intron
TGGTAATACAGTTTCACGGG+TGG	0.672568	8:+84391944	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATAAATAAAATAAAAA+TGG	-	Chr8:84394042-84394061	MsG0880047306.01.T01:CDS	0.0%
!!	AAAATAAATAAAATAAAAAT+GGG	-	Chr8:84394043-84394062	MsG0880047306.01.T01:CDS	0.0%
!!	ATGACAAATAAATAAACAAA+AGG	-	Chr8:84394000-84394019	MsG0880047306.01.T01:CDS	15.0%
!!	CATAAATCTGATAAAATATT+TGG	+	Chr8:84393242-84393261	None:intergenic	15.0%
!!!	GATAAATATTTAAAATAAGC+CGG	+	Chr8:84393104-84393123	None:intergenic	15.0%
!!!	TTGTTTTAATACAACAATAA+AGG	-	Chr8:84395040-84395059	MsG0880047306.01.T01:CDS	15.0%
!!	AATTCCAGTATTATATCTTT+AGG	-	Chr8:84394227-84394246	MsG0880047306.01.T01:intron	20.0%
!!	ACATATCAATTATTTAGACT+AGG	+	Chr8:84393366-84393385	None:intergenic	20.0%
!!	ATATATATATTGTGTGCATA+TGG	-	Chr8:84394252-84394271	MsG0880047306.01.T01:intron	20.0%
!!	ATTACCTAAAGATATAATAC+TGG	+	Chr8:84394234-84394253	None:intergenic	20.0%
!!	ATTGAACATATCAATCAAAT+GGG	+	Chr8:84394596-84394615	None:intergenic	20.0%
!!	TAAAAATGTAAACAGAGATA+GGG	+	Chr8:84393827-84393846	None:intergenic	20.0%
!!	TAAAGCATGAAAATTAACAA+GGG	+	Chr8:84393712-84393731	None:intergenic	20.0%
!!	TTAAAAATGTAAACAGAGAT+AGG	+	Chr8:84393828-84393847	None:intergenic	20.0%
!!	TTGTAAATCAAAAATTGAGA+GGG	+	Chr8:84394850-84394869	None:intergenic	20.0%
!!	TTTATTTATTTGTCATTCTC+TGG	+	Chr8:84393996-84394015	None:intergenic	20.0%
!!	TTTGTAAATCAAAAATTGAG+AGG	+	Chr8:84394851-84394870	None:intergenic	20.0%
!!!	ATTATATTTCACTTTTCCAA+GGG	+	Chr8:84392875-84392894	None:intergenic	20.0%
!!!	TTATTTTTAGGGTCAATATA+GGG	+	Chr8:84394935-84394954	None:intergenic	20.0%
!!!	TTTATTTTTAGGGTCAATAT+AGG	+	Chr8:84394936-84394955	None:intergenic	20.0%
!	AAACCATGAAGCTAAAATTA+GGG	+	Chr8:84393735-84393754	None:intergenic	25.0%
!	AATACACTATACGAAGAAAA+TGG	-	Chr8:84392221-84392240	MsG0880047306.01.T01:intron	25.0%
!	AATTACTAGGAATGTTCAAA+GGG	-	Chr8:84394774-84394793	MsG0880047306.01.T01:intron	25.0%
!	ATCTGATGAAACCATTTATT+TGG	+	Chr8:84393315-84393334	None:intergenic	25.0%
!	ATGTCATACAGAATATATAC+AGG	-	Chr8:84393212-84393231	MsG0880047306.01.T01:intron	25.0%
!	CATTGAACATATCAATCAAA+TGG	+	Chr8:84394597-84394616	None:intergenic	25.0%
!	GTAAAGCATGAAAATTAACA+AGG	+	Chr8:84393713-84393732	None:intergenic	25.0%
!	GTTATGGAAAATCTTCTTAT+CGG	-	Chr8:84393483-84393502	MsG0880047306.01.T01:intron	25.0%
!	TACAAATCATAACTTTCTGA+AGG	-	Chr8:84394865-84394884	MsG0880047306.01.T01:CDS	25.0%
!	TAGGAAGTAACAATAAAGAA+AGG	+	Chr8:84394905-84394924	None:intergenic	25.0%
!	TATCAGAAAGTGAAATCAAT+TGG	-	Chr8:84391844-84391863	MsG0880047306.01.T02:three_prime_UTR	25.0%
!	TTGTATTATGTGATAAATGC+AGG	-	Chr8:84394707-84394726	MsG0880047306.01.T01:CDS	25.0%
!!	AAGAGTAATTTTACCTTGAT+TGG	+	Chr8:84391803-84391822	None:intergenic	25.0%
!!	AATCTTGATAATTATACTGC+TGG	-	Chr8:84392311-84392330	MsG0880047306.01.T01:intron	25.0%
!!	AATTGATTTCACTTTCTGAT+AGG	+	Chr8:84391845-84391864	None:intergenic	25.0%
!!	TTCTTTATTGTTACTTCCTA+CGG	-	Chr8:84394905-84394924	MsG0880047306.01.T01:CDS	25.0%
!!!	AAAAATGGATGTTTTGGTTA+TGG	-	Chr8:84393467-84393486	MsG0880047306.01.T01:CDS	25.0%
!!!	ACAGTTTCTAAAATATAGGA+TGG	+	Chr8:84393267-84393286	None:intergenic	25.0%
!!!	CATTATATTTCACTTTTCCA+AGG	+	Chr8:84392876-84392895	None:intergenic	25.0%
!!!	TTATGTCTTGTTTTCTTTGA+AGG	-	Chr8:84393411-84393430	MsG0880047306.01.T01:CDS	25.0%
!!!	TTTTGATTGTGATGTTAGAA+AGG	-	Chr8:84393784-84393803	MsG0880047306.01.T01:CDS	25.0%
	AAAAAGAGAACAGACATGAA+TGG	+	Chr8:84394658-84394677	None:intergenic	30.0%
	AAAGATCTTGGGAATTTATC+AGG	-	Chr8:84393160-84393179	MsG0880047306.01.T01:intron	30.0%
	AACAAGTTTCTGTGTATTGA+AGG	+	Chr8:84392453-84392472	None:intergenic	30.0%
	AAGAATAGCTAAAACTGGTT+TGG	-	Chr8:84393864-84393883	MsG0880047306.01.T01:CDS	30.0%
	AATCATTATCAAGTTTGCAC+CGG	-	Chr8:84393082-84393101	MsG0880047306.01.T01:intron	30.0%
	AATGAACATGAGTTAGCAAA+AGG	+	Chr8:84392416-84392435	None:intergenic	30.0%
	AATGCAGCAGAAATATATTG+AGG	+	Chr8:84394689-84394708	None:intergenic	30.0%
	ACTCTCGTAATGATAAAATG+TGG	+	Chr8:84392489-84392508	None:intergenic	30.0%
	ATGCAGCAGAAATATATTGA+GGG	+	Chr8:84394688-84394707	None:intergenic	30.0%
	CAATTACTAGGAATGTTCAA+AGG	-	Chr8:84394773-84394792	MsG0880047306.01.T01:intron	30.0%
	CATGAAAATCCTTTGAATAC+AGG	+	Chr8:84392826-84392845	None:intergenic	30.0%
	CTCTTATGCAACCAAATAAA+TGG	-	Chr8:84393301-84393320	MsG0880047306.01.T01:CDS	30.0%
	CTTTGTGATGAAAATTCATG+AGG	-	Chr8:84394565-84394584	MsG0880047306.01.T01:CDS	30.0%
	GAAACCATGAAGCTAAAATT+AGG	+	Chr8:84393736-84393755	None:intergenic	30.0%
	GAACAAAGAATAGCTAAAAC+TGG	-	Chr8:84393859-84393878	MsG0880047306.01.T01:CDS	30.0%
	GAGAAAGATCAAATTTACTC+TGG	+	Chr8:84392047-84392066	None:intergenic	30.0%
	GATAATTATACTGCTGGTAT+AGG	-	Chr8:84392317-84392336	MsG0880047306.01.T01:intron	30.0%
	TAAATTAAACTCTCCGAGTT+TGG	-	Chr8:84392092-84392111	MsG0880047306.01.T01:three_prime_UTR	30.0%
	TGAACATTCCTAGTAATTGA+AGG	+	Chr8:84394772-84394791	None:intergenic	30.0%
	TGTGTTCCTTAGAAAAAACA+TGG	-	Chr8:84394738-84394757	MsG0880047306.01.T01:intron	30.0%
	TTAGAAAGGAAAACTCACAT+AGG	-	Chr8:84393798-84393817	MsG0880047306.01.T01:CDS	30.0%
	TTCCTTAGAAAAAACATGGT+AGG	-	Chr8:84394742-84394761	MsG0880047306.01.T01:intron	30.0%
!	ATTCAAAGGATTTTCATGCA+AGG	-	Chr8:84392828-84392847	MsG0880047306.01.T01:intron	30.0%
!	ATTTTATGAGGCAGTATACA+TGG	-	Chr8:84394356-84394375	MsG0880047306.01.T01:intron	30.0%
!	CATTTTATCATTACGAGAGT+TGG	-	Chr8:84392489-84392508	MsG0880047306.01.T01:three_prime_UTR	30.0%
!	CTCATGAATTTTCATCACAA+AGG	+	Chr8:84394567-84394586	None:intergenic	30.0%
!	GAATTCGAGATCAGTTATTT+TGG	-	Chr8:84395108-84395127	MsG0880047306.01.T01:CDS	30.0%
!	GATGAGCTTATATTTTCTGA+TGG	-	Chr8:84394489-84394508	MsG0880047306.01.T01:CDS	30.0%
!	GGAAACAAAAATGGATGTTT+TGG	-	Chr8:84393461-84393480	MsG0880047306.01.T01:CDS	30.0%
!	TCGGTTCAGTTTTTATACAT+TGG	-	Chr8:84394169-84394188	MsG0880047306.01.T01:intron	30.0%
!	TCTTCGTATAGTGTATTTTC+CGG	+	Chr8:84392218-84392237	None:intergenic	30.0%
!	TGAATTTTCATCACAAAGGT+CGG	+	Chr8:84394563-84394582	None:intergenic	30.0%
!	TGTTTTCTTTGCAAAAACCT+TGG	-	Chr8:84393017-84393036	MsG0880047306.01.T01:intron	30.0%
!	TTCAAAGGATTTTCATGCAA+GGG	-	Chr8:84392829-84392848	MsG0880047306.01.T01:intron	30.0%
!	TTTTATGAGGCAGTATACAT+GGG	-	Chr8:84394357-84394376	MsG0880047306.01.T01:intron	30.0%
!!	AGTGAAATCAATTGGTTCTA+AGG	-	Chr8:84391852-84391871	MsG0880047306.01.T02:three_prime_UTR	30.0%
!!	ATCAAGGGTATCAGATAAAT+TGG	+	Chr8:84392171-84392190	None:intergenic	30.0%
!!	ATCAATTGGTTCTAAGGAAA+AGG	-	Chr8:84391858-84391877	MsG0880047306.01.T02:three_prime_UTR	30.0%
!!	TGCGACAGTTTCTAAAATAT+AGG	+	Chr8:84393271-84393290	None:intergenic	30.0%
!!!	AGGTTTTGTTTCCAAAAGAA+AGG	+	Chr8:84391938-84391957	None:intergenic	30.0%
!!!	CTATCACAATGTTTTGAAAC+CGG	-	Chr8:84393969-84393988	MsG0880047306.01.T01:CDS	30.0%
!!!	TAGGCATCATTGATTTTATG+AGG	-	Chr8:84394344-84394363	MsG0880047306.01.T01:intron	30.0%
!!!	TTACCCTAATTTTAGCTTCA+TGG	-	Chr8:84393729-84393748	MsG0880047306.01.T01:intron	30.0%
	AACTTGTCTTGCAAAGATCT+TGG	-	Chr8:84393148-84393167	MsG0880047306.01.T01:intron	35.0%
	AAGATCTTTGCAAGACAAGT+TGG	+	Chr8:84393149-84393168	None:intergenic	35.0%
	AATTATACTGCTGGTATAGG+TGG	-	Chr8:84392320-84392339	MsG0880047306.01.T01:intron	35.0%
	ACATGTGCTATAACACTAGT+CGG	+	Chr8:84392589-84392608	None:intergenic	35.0%
	ACTTGTCTTGCAAAGATCTT+GGG	-	Chr8:84393149-84393168	MsG0880047306.01.T01:intron	35.0%
	AGATCTTTGCAAGACAAGTT+GGG	+	Chr8:84393148-84393167	None:intergenic	35.0%
	AGCAACATAATCACTTGAGA+TGG	-	Chr8:84395017-84395036	MsG0880047306.01.T01:CDS	35.0%
	AGCATGGTAATACAGTTTCA+CGG	+	Chr8:84394966-84394985	None:intergenic	35.0%
	AGCCTTAAATATAGTCTCAC+TGG	+	Chr8:84394989-84395008	None:intergenic	35.0%
	ATGGTATCATTAATGCCAAC+AGG	+	Chr8:84392544-84392563	None:intergenic	35.0%
	CTACTATGCAAAGAGATTTG+AGG	-	Chr8:84392903-84392922	MsG0880047306.01.T01:intron	35.0%
	CTCCAGTGAGACTATATTTA+AGG	-	Chr8:84394984-84395003	MsG0880047306.01.T01:CDS	35.0%
	CTGATATATGCATCCAATCA+AGG	-	Chr8:84391787-84391806	MsG0880047306.01.T02:three_prime_UTR	35.0%
	CTTGATTGGATGCATATATC+AGG	+	Chr8:84391789-84391808	None:intergenic	35.0%
	GAACTTTCTAGACTGTCTTT+AGG	+	Chr8:84392658-84392677	None:intergenic	35.0%
	GACGATAAATCTATCATCCA+AGG	+	Chr8:84393037-84393056	None:intergenic	35.0%
	GACTCTAAATTGCATTCCAA+AGG	-	Chr8:84392365-84392384	MsG0880047306.01.T01:three_prime_UTR	35.0%
	GTTGCAGTCAATATATGTGA+TGG	-	Chr8:84394274-84394293	MsG0880047306.01.T01:intron	35.0%
	TCTAAATCAAGGTTTCCATC+AGG	+	Chr8:84391958-84391977	None:intergenic	35.0%
	TCTGTATGACATCAGAACAT+TGG	+	Chr8:84393204-84393223	None:intergenic	35.0%
	TGATAGATTTATCGTCAAGC+AGG	-	Chr8:84393041-84393060	MsG0880047306.01.T01:intron	35.0%
	TGATGAAAATTCATGAGGTG+TGG	-	Chr8:84394570-84394589	MsG0880047306.01.T01:CDS	35.0%
	TGTTCCAACAAATATTGGAG+AGG	+	Chr8:84393623-84393642	None:intergenic	35.0%
	TTATTTGTCATTCTCTGGTC+CGG	+	Chr8:84393991-84394010	None:intergenic	35.0%
	TTTAATGATGACAGTTCCCT+TGG	-	Chr8:84392856-84392875	MsG0880047306.01.T01:intron	35.0%
	TTTGATCTTTCTCTCTCTCA+TGG	-	Chr8:84392054-84392073	MsG0880047306.01.T01:three_prime_UTR	35.0%
!	CTCCAATATTTGTTGGAACA+AGG	-	Chr8:84393623-84393642	MsG0880047306.01.T01:intron	35.0%
!	CTTCGTATAGTGTATTTTCC+GGG	+	Chr8:84392217-84392236	None:intergenic	35.0%
!	GGCTAGAGTTTTCTAAATCA+AGG	+	Chr8:84391969-84391988	None:intergenic	35.0%
!	TGCCTTGTTCCAACAAATAT+TGG	+	Chr8:84393628-84393647	None:intergenic	35.0%
!!	AACAGTTGGAGAAGTATTCT+TGG	+	Chr8:84394431-84394450	None:intergenic	35.0%
!!	ACAGTTGGAGAAGTATTCTT+GGG	+	Chr8:84394430-84394449	None:intergenic	35.0%
!!	CAGTTCTGATTCCTTTCTTT+TGG	-	Chr8:84391924-84391943	MsG0880047306.01.T02:three_prime_UTR	35.0%
!!	CTTTTGGAAACAAAACCTGA+TGG	-	Chr8:84391940-84391959	MsG0880047306.01.T02:three_prime_UTR	35.0%
!!!	ACGGGTGGATCTTTATTTTT+AGG	+	Chr8:84394947-84394966	None:intergenic	35.0%
!!!	CACCTACCATGTTTTTTCTA+AGG	+	Chr8:84394747-84394766	None:intergenic	35.0%
!!!	CGGGTGGATCTTTATTTTTA+GGG	+	Chr8:84394946-84394965	None:intergenic	35.0%
	AAAGGAACTTCCTGATGCAA+TGG	-	Chr8:84395058-84395077	MsG0880047306.01.T01:CDS	40.0%
	AATATAGTCTCACTGGAGCA+TGG	+	Chr8:84394982-84395001	None:intergenic	40.0%
	AATTGCAGAACCATTGCATC+AGG	+	Chr8:84395071-84395090	None:intergenic	40.0%
	ACAGAAGTTTGGATCATCTG+AGG	-	Chr8:84392751-84392770	MsG0880047306.01.T01:intron	40.0%
	AGATCAAACCAACCATGAAC+TGG	-	Chr8:84394126-84394145	MsG0880047306.01.T01:intron	40.0%
	AGGAGGGAAGGAAACAAAAA+TGG	-	Chr8:84393452-84393471	MsG0880047306.01.T01:CDS	40.0%
	AGGCTTGTCGAATCAAATAG+TGG	+	Chr8:84392692-84392711	None:intergenic	40.0%
	AGGTACAATCCAGATACAAG+TGG	-	Chr8:84393551-84393570	MsG0880047306.01.T01:intron	40.0%
	ATCATCATAGACTGGCACTA+TGG	+	Chr8:84392563-84392582	None:intergenic	40.0%
	ATCTCAGTTGTACTGTAGTC+TGG	+	Chr8:84394110-84394129	None:intergenic	40.0%
	ATTAAACTCTCCGAGTTTGG+AGG	-	Chr8:84392095-84392114	MsG0880047306.01.T01:three_prime_UTR	40.0%
	CAGTCTGGTGAAATGATCAA+AGG	-	Chr8:84391997-84392016	MsG0880047306.01.T02:three_prime_UTR	40.0%
	CCACTTCTTACAACGACTAA+GGG	+	Chr8:84392974-84392993	None:intergenic	40.0%
	CCTTAGTCGTTGTAAGAAGT+GGG	-	Chr8:84392972-84392991	MsG0880047306.01.T01:intron	40.0%
	CTTAGTCGTTGTAAGAAGTG+GGG	-	Chr8:84392973-84392992	MsG0880047306.01.T01:intron	40.0%
	CTTTGATCATTTCACCAGAC+TGG	+	Chr8:84391999-84392018	None:intergenic	40.0%
	GAAAGGAATCAGAACTGCTA+TGG	+	Chr8:84391921-84391940	None:intergenic	40.0%
	GAATTCTTGATGCCAGCTAA+TGG	+	Chr8:84395094-84395113	None:intergenic	40.0%
	GATAAATCCTACATGCACGA+TGG	+	Chr8:84393890-84393909	None:intergenic	40.0%
	GCACAAAATCAAGCTCAGAA+GGG	+	Chr8:84392948-84392967	None:intergenic	40.0%
	GCATGGTAATACAGTTTCAC+GGG	+	Chr8:84394965-84394984	None:intergenic	40.0%
	GGATTCACTCCTGTATTCAA+AGG	-	Chr8:84392814-84392833	MsG0880047306.01.T01:intron	40.0%
	GGTACAATCCAGATACAAGT+GGG	-	Chr8:84393552-84393571	MsG0880047306.01.T01:intron	40.0%
	GTCATGAGTGTATCCTGTTT+CGG	+	Chr8:84392348-84392367	None:intergenic	40.0%
	GTCTATCAGATTCTGACATC+TGG	+	Chr8:84392636-84392655	None:intergenic	40.0%
	TACGAACAGCTACAGAAGTT+TGG	-	Chr8:84392740-84392759	MsG0880047306.01.T01:intron	40.0%
	TAGGTGCACCTTCAATTACT+AGG	-	Chr8:84394761-84394780	MsG0880047306.01.T01:intron	40.0%
	TCAAAGGTTCTTCTCGATCA+AGG	-	Chr8:84393531-84393550	MsG0880047306.01.T01:intron	40.0%
	TCCAAAGAGTGAGAGAAAGT+AGG	+	Chr8:84392712-84392731	None:intergenic	40.0%
	TCCAACAAATATTGGAGAGG+TGG	+	Chr8:84393620-84393639	None:intergenic	40.0%
	TGCTGTGGTGATATAACAGT+TGG	+	Chr8:84394445-84394464	None:intergenic	40.0%
	TGGTGAAATGATCAAAGGTG+AGG	-	Chr8:84392002-84392021	MsG0880047306.01.T02:three_prime_UTR	40.0%
	TGTGACAGTTCATTCACCTA+CGG	-	Chr8:84392268-84392287	MsG0880047306.01.T01:intron	40.0%
	TTAGTCGTTGTAAGAAGTGG+GGG	-	Chr8:84392974-84392993	MsG0880047306.01.T01:intron	40.0%
	TTATCAGAGTCGATCAGTAC+AGG	-	Chr8:84393119-84393138	MsG0880047306.01.T01:intron	40.0%
	TTATTGCTCCCACTTGTATC+TGG	+	Chr8:84393563-84393582	None:intergenic	40.0%
	TTTCATCACAAAGGTCGGTT+TGG	+	Chr8:84394558-84394577	None:intergenic	40.0%
	TTTGCAGTAGCTATTACCGT+AGG	+	Chr8:84392287-84392306	None:intergenic	40.0%
	TTTGTTGGAACAAGGCAAAG+CGG	-	Chr8:84393631-84393650	MsG0880047306.01.T01:intron	40.0%
!	AGACGGCTTTATGATCTCAA+AGG	-	Chr8:84393515-84393534	MsG0880047306.01.T01:intron	40.0%
!	ATGGACTACTCATTGCTTGT+TGG	-	Chr8:84394293-84394312	MsG0880047306.01.T01:intron	40.0%
!	CTTCTGAGCTTGATTTTGTG+CGG	-	Chr8:84392947-84392966	MsG0880047306.01.T01:intron	40.0%
!	TTCATCACAAAGGTCGGTTT+GGG	+	Chr8:84394557-84394576	None:intergenic	40.0%
!!	GATTCACTGGTTTGTAAGCT+TGG	+	Chr8:84394535-84394554	None:intergenic	40.0%
!!	TGGTTCTAAGGAAAAGGTTG+TGG	-	Chr8:84391864-84391883	MsG0880047306.01.T02:three_prime_UTR	40.0%
!!!	TCGAGATCAGTTATTTTGGC+CGG	-	Chr8:84395112-84395131	MsG0880047306.01.T01:CDS	40.0%
	AAAGGTTGTGGAGACAGATG+TGG	-	Chr8:84391876-84391895	MsG0880047306.01.T02:three_prime_UTR	45.0%
	AACCGTTTCTTGTACTGCTG+TGG	+	Chr8:84394460-84394479	None:intergenic	45.0%
	AGCAGTACAAGAAACGGTTC+AGG	-	Chr8:84394461-84394480	MsG0880047306.01.T01:CDS	45.0%
	AGCATGACGTTATCCACTTC+TGG	-	Chr8:84392776-84392795	MsG0880047306.01.T01:intron	45.0%
	AGGTCTTCACCCAAGTTTCA+AGG	+	Chr8:84394390-84394409	None:intergenic	45.0%
	ATTGAACCAGGGATGAACTG+TGG	+	Chr8:84394078-84394097	None:intergenic	45.0%
	CACCACAGCAGTACAAGAAA+CGG	-	Chr8:84394455-84394474	MsG0880047306.01.T01:CDS	45.0%
	CCAAAGGTCTTGACACTAGA+TGG	-	Chr8:84392381-84392400	MsG0880047306.01.T01:three_prime_UTR	45.0%
	CCATCTAGTGTCAAGACCTT+TGG	+	Chr8:84392384-84392403	None:intergenic	45.0%
	CCCACTTCTTACAACGACTA+AGG	+	Chr8:84392975-84392994	None:intergenic	45.0%
	CCCTTAGTCGTTGTAAGAAG+TGG	-	Chr8:84392971-84392990	MsG0880047306.01.T01:intron	45.0%
	CGCACAAAATCAAGCTCAGA+AGG	+	Chr8:84392949-84392968	None:intergenic	45.0%
	CTTATCGGCGCAACATAAGA+CGG	-	Chr8:84393498-84393517	MsG0880047306.01.T01:intron	45.0%
	GAGGACAAGAGTAAAGACAC+AGG	-	Chr8:84392021-84392040	MsG0880047306.01.T02:three_prime_UTR	45.0%
	GCATGACGTTATCCACTTCT+GGG	-	Chr8:84392777-84392796	MsG0880047306.01.T01:intron	45.0%
	GCCACCTCTCCAATATTTGT+TGG	-	Chr8:84393616-84393635	MsG0880047306.01.T01:intron	45.0%
	GCCTACTTTCTCTCACTCTT+TGG	-	Chr8:84392708-84392727	MsG0880047306.01.T01:intron	45.0%
	GGACAAGCACCTTGAAACTT+GGG	-	Chr8:84394378-84394397	MsG0880047306.01.T01:intron	45.0%
	GGTAGAACTGGATTGAACCA+GGG	+	Chr8:84394089-84394108	None:intergenic	45.0%
	GGTTCTGCAATTCCATTAGC+TGG	-	Chr8:84395079-84395098	MsG0880047306.01.T01:CDS	45.0%
	GTACTGTAGTCTGGTAGAAC+TGG	+	Chr8:84394101-84394120	None:intergenic	45.0%
	GTCAATATAGGGACTTCCGT+AGG	+	Chr8:84394924-84394943	None:intergenic	45.0%
	GTCTTGACACTAGATGGTCT+GGG	-	Chr8:84392387-84392406	MsG0880047306.01.T01:three_prime_UTR	45.0%
	TAGTCGTTGTAAGAAGTGGG+GGG	-	Chr8:84392975-84392994	MsG0880047306.01.T01:intron	45.0%
	TATGCTGGATCATGTCTGCA+AGG	-	Chr8:84394797-84394816	MsG0880047306.01.T01:intron	45.0%
	TGGGAGCAATAAAGTTCTGC+TGG	-	Chr8:84393571-84393590	MsG0880047306.01.T01:intron	45.0%
	TGGTAATACAGTTTCACGGG+TGG	+	Chr8:84394962-84394981	None:intergenic	45.0%
	TGGTAGAACTGGATTGAACC+AGG	+	Chr8:84394090-84394109	None:intergenic	45.0%
	TTGTTTCCTTCCCTCCTTTG+AGG	+	Chr8:84393449-84393468	None:intergenic	45.0%
!	ACATCAAAGCACCTCAAAGG+AGG	-	Chr8:84393435-84393454	MsG0880047306.01.T01:CDS	45.0%
!	ATTGCTTGTTGGTGTGGATG+AGG	-	Chr8:84394304-84394323	MsG0880047306.01.T01:intron	45.0%
!	CATCAAAGCACCTCAAAGGA+GGG	-	Chr8:84393436-84393455	MsG0880047306.01.T01:CDS	45.0%
!	CGTATAGTGTATTTTCCGGG+TGG	+	Chr8:84392214-84392233	None:intergenic	45.0%
!	CTACTCATTGCTTGTTGGTG+TGG	-	Chr8:84394298-84394317	MsG0880047306.01.T01:intron	45.0%
!	GGAATGTTCAAAGGGTATGC+TGG	-	Chr8:84394782-84394801	MsG0880047306.01.T01:intron	45.0%
!	GGAGAAGCATGAGCTAGTTT+AGG	-	Chr8:84394325-84394344	MsG0880047306.01.T01:intron	45.0%
!	GGTACATCAAAGCACCTCAA+AGG	-	Chr8:84393432-84393451	MsG0880047306.01.T01:CDS	45.0%
!	GTCGGTTCATCATCATAGAC+TGG	+	Chr8:84392571-84392590	None:intergenic	45.0%
!	TCATTTCACCAGACTGGCTA+AGG	+	Chr8:84391993-84392012	None:intergenic	45.0%
!	TTCTGATGGTGCCAGATCAA+TGG	-	Chr8:84394503-84394522	MsG0880047306.01.T01:CDS	45.0%
!!	TCACTGGTTTGTAAGCTTGG+AGG	+	Chr8:84394532-84394551	None:intergenic	45.0%
!!!	CGAGATCAGTTATTTTGGCC+GGG	-	Chr8:84395113-84395132	MsG0880047306.01.T01:CDS	45.0%
	AACCAACCACAGTTCATCCC+TGG	-	Chr8:84394069-84394088	MsG0880047306.01.T01:intron	50.0%
	AACCAGGGATGAACTGTGGT+TGG	+	Chr8:84394074-84394093	None:intergenic	50.0%
	AAGACCTCAGGCATTCTTGG+TGG	-	Chr8:84394403-84394422	MsG0880047306.01.T01:CDS	50.0%
	CGTTATCCACTTCTGGGTCT+CGG	-	Chr8:84392783-84392802	MsG0880047306.01.T01:intron	50.0%
	CTGATACCCTTGATGCAGCA+TGG	-	Chr8:84392177-84392196	MsG0880047306.01.T01:three_prime_UTR	50.0%
	CTTGGAGGAGACCATTGATC+TGG	+	Chr8:84394517-84394536	None:intergenic	50.0%
	GAAACTTGGGTGAAGACCTC+AGG	-	Chr8:84394391-84394410	MsG0880047306.01.T01:intron	50.0%
	GCTAGACTGCTTCTTCCTGT+TGG	-	Chr8:84392526-84392545	MsG0880047306.01.T01:three_prime_UTR	50.0%
	GGGACAAGCACCTTGAAACT+TGG	-	Chr8:84394377-84394396	MsG0880047306.01.T01:intron	50.0%
	GGGCAATCAGACTTACAGCA+AGG	+	Chr8:84393692-84393711	None:intergenic	50.0%
	GGTATAGGTGGAACCGAAAC+AGG	-	Chr8:84392332-84392351	MsG0880047306.01.T01:intron	50.0%
	GGTCTTGACACTAGATGGTC+TGG	-	Chr8:84392386-84392405	MsG0880047306.01.T01:three_prime_UTR	50.0%
	GTGAAGACCTCAGGCATTCT+TGG	-	Chr8:84394400-84394419	MsG0880047306.01.T01:intron	50.0%
	TGGAGACAGATGTGGTCACT+AGG	-	Chr8:84391884-84391903	MsG0880047306.01.T02:three_prime_UTR	50.0%
	TGGATGCATATATCAGGCGC+TGG	+	Chr8:84391783-84391802	None:intergenic	50.0%
!	AAAGCACCTCAAAGGAGGGA+AGG	-	Chr8:84393440-84393459	MsG0880047306.01.T01:CDS	50.0%
!	TTTCACCAGACTGGCTAAGG+TGG	+	Chr8:84391990-84392009	None:intergenic	50.0%
!!	AAGGTCGGTTTGGGATTCAC+TGG	+	Chr8:84394548-84394567	None:intergenic	50.0%
!!	CAGCTTCTGGTTCAACTGGT+CGG	-	Chr8:84394150-84394169	MsG0880047306.01.T01:intron	50.0%
	AACCATCCGAGACCCAGAAG+TGG	+	Chr8:84392792-84392811	None:intergenic	55.0%
	ACAGCTGCTACATCACGAGC+TGG	+	Chr8:84392251-84392270	None:intergenic	55.0%
	ACCAGAAGCTGGCCAGTTCA+TGG	+	Chr8:84394141-84394160	None:intergenic	55.0%
	ACCATGAACTGGCCAGCTTC+TGG	-	Chr8:84394137-84394156	MsG0880047306.01.T01:intron	55.0%
	ACCCTTGATGCAGCATGGAC+TGG	-	Chr8:84392182-84392201	MsG0880047306.01.T01:intron	55.0%
	ACTTCTGGGTCTCGGATGGT+TGG	-	Chr8:84392791-84392810	MsG0880047306.01.T01:intron	55.0%
	AGTTTGGAGGAGCTATCCGG+AGG	-	Chr8:84392108-84392127	MsG0880047306.01.T01:three_prime_UTR	55.0%
	ATCCACTTCTGGGTCTCGGA+TGG	-	Chr8:84392787-84392806	MsG0880047306.01.T01:intron	55.0%
	CACATTACGAGATCCCCCCT+CGG	+	Chr8:84392147-84392166	None:intergenic	55.0%
	CCCAGTCCATGCTGCATCAA+GGG	+	Chr8:84392186-84392205	None:intergenic	55.0%
	CCCTTGATGCAGCATGGACT+GGG	-	Chr8:84392183-84392202	MsG0880047306.01.T01:intron	55.0%
	CCGAGTTTGGAGGAGCTATC+CGG	-	Chr8:84392105-84392124	MsG0880047306.01.T01:three_prime_UTR	55.0%
	CCGGATAGCTCCTCCAAACT+CGG	+	Chr8:84392108-84392127	None:intergenic	55.0%
	CGACCAGTTGAACCAGAAGC+TGG	+	Chr8:84394152-84394171	None:intergenic	55.0%
	CTTCTGGGTCTCGGATGGTT+GGG	-	Chr8:84392792-84392811	MsG0880047306.01.T01:intron	55.0%
	GAAGCTGGCCAGTTCATGGT+TGG	+	Chr8:84394137-84394156	None:intergenic	55.0%
	GGCTTCTTGAGAGAGCTGTG+TGG	-	Chr8:84393652-84393671	MsG0880047306.01.T01:intron	55.0%
	GGTTTGGCCATCGTGCATGT+AGG	-	Chr8:84393880-84393899	MsG0880047306.01.T01:CDS	55.0%
	TCTAGCCACCTTAGCCAGTC+TGG	-	Chr8:84391982-84392001	MsG0880047306.01.T02:three_prime_UTR	55.0%
	TGTAAGAAGTGGGGGGCTCA+GGG	-	Chr8:84392982-84393001	MsG0880047306.01.T01:intron	55.0%
	TTCTGGGTCTCGGATGGTTG+GGG	-	Chr8:84392793-84392812	MsG0880047306.01.T01:intron	55.0%
	TTGTAAGAAGTGGGGGGCTC+AGG	-	Chr8:84392981-84393000	MsG0880047306.01.T01:intron	55.0%
!	CCTTGATGCAGCATGGACTG+GGG	-	Chr8:84392184-84392203	MsG0880047306.01.T01:intron	55.0%
!!	TGGCCAGCTTCTGGTTCAAC+TGG	-	Chr8:84394146-84394165	MsG0880047306.01.T01:intron	55.0%
	ACGAGAGTTGGACCGCCAGA+CGG	-	Chr8:84392501-84392520	MsG0880047306.01.T01:three_prime_UTR	60.0%
	AGGAGGAGGACTTCGTCCGA+GGG	-	Chr8:84392128-84392147	MsG0880047306.01.T01:three_prime_UTR	60.0%
	CCCCAGTCCATGCTGCATCA+AGG	+	Chr8:84392187-84392206	None:intergenic	60.0%
	CGAGGGGGGATCTCGTAATG+TGG	-	Chr8:84392145-84392164	MsG0880047306.01.T01:three_prime_UTR	60.0%
	GAAGCAGTCTAGCACCCGTC+TGG	+	Chr8:84392519-84392538	None:intergenic	60.0%
	GGGTCCACCAAGAATGCCTG+AGG	+	Chr8:84394410-84394429	None:intergenic	60.0%
	TTGGAGGAGCTATCCGGAGG+AGG	-	Chr8:84392111-84392130	MsG0880047306.01.T01:three_prime_UTR	60.0%
!!	ATGGACTGGGGAGAACCACC+CGG	-	Chr8:84392196-84392215	MsG0880047306.01.T01:intron	60.0%
	AAGAAGTGGGGGGCTCAGGG+AGG	-	Chr8:84392985-84393004	MsG0880047306.01.T01:intron	65.0%
	AGGAGGACTTCGTCCGAGGG+GGG	-	Chr8:84392131-84392150	MsG0880047306.01.T01:three_prime_UTR	65.0%
	CGAGAGTTGGACCGCCAGAC+GGG	-	Chr8:84392502-84392521	MsG0880047306.01.T01:three_prime_UTR	65.0%
	CGGACGAAGTCCTCCTCCTC+CGG	+	Chr8:84392127-84392146	None:intergenic	65.0%
	GAGGAGCTATCCGGAGGAGG+AGG	-	Chr8:84392114-84392133	MsG0880047306.01.T01:three_prime_UTR	65.0%
	GAGGAGGACTTCGTCCGAGG+GGG	-	Chr8:84392130-84392149	MsG0880047306.01.T01:three_prime_UTR	65.0%
	GAGGAGGAGGACTTCGTCCG+AGG	-	Chr8:84392127-84392146	MsG0880047306.01.T01:three_prime_UTR	65.0%
	GCAGTCTAGCACCCGTCTGG+CGG	+	Chr8:84392516-84392535	None:intergenic	65.0%
	GGAGGAGGACTTCGTCCGAG+GGG	-	Chr8:84392129-84392148	MsG0880047306.01.T01:three_prime_UTR	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	84391775	84395150	84391775	ID=MsG0880047306.01;Name=MsG0880047306.01
Chr8	mRNA	84392023	84395150	84392023	ID=MsG0880047306.01.T01;Parent=MsG0880047306.01;Name=MsG0880047306.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|0.6\|0.5\|1\|0.6\|0.5\|6\|514\|407
Chr8	exon	84392023	84392196	84392023	ID=MsG0880047306.01.T01:exon:330;Parent=MsG0880047306.01.T01
Chr8	exon	84392339	84392644	84392339	ID=MsG0880047306.01.T01:exon:329;Parent=MsG0880047306.01.T01
Chr8	CDS	84394817	84395150	84394817	ID=MsG0880047306.01.T01:cds;Parent=MsG0880047306.01.T01
Chr8	CDS	84394402	84394730	84394402	ID=MsG0880047306.01.T01:cds;Parent=MsG0880047306.01.T01
Chr8	CDS	84393744	84394075	84393744	ID=MsG0880047306.01.T01:cds;Parent=MsG0880047306.01.T01
Chr8	CDS	84393264	84393492	84393264	ID=MsG0880047306.01.T01:cds;Parent=MsG0880047306.01.T01
Chr8	three_prime_UTR	84393230	84393263	84393230	ID=MsG0880047306.01.T01:three_prime_utr;Parent=MsG0880047306.01.T01
Chr8	three_prime_UTR	84392339	84392644	84392339	ID=MsG0880047306.01.T01:three_prime_utr;Parent=MsG0880047306.01.T01
Chr8	three_prime_UTR	84392023	84392196	84392023	ID=MsG0880047306.01.T01:three_prime_utr;Parent=MsG0880047306.01.T01
Chr8	mRNA	84391775	84395150	84391775	ID=MsG0880047306.01.T02;Parent=MsG0880047306.01;Name=MsG0880047306.01.T02;_AED=0.47;_eAED=0.47;_QI=0\|0.5\|0.4\|1\|0.75\|0.6\|5\|904\|407
Chr8	exon	84391775	84392644	84391775	ID=MsG0880047306.01.T02:exon:331;Parent=MsG0880047306.01.T02
Chr8	CDS	84394817	84395150	84394817	ID=MsG0880047306.01.T02:cds;Parent=MsG0880047306.01.T02
Chr8	CDS	84394402	84394730	84394402	ID=MsG0880047306.01.T02:cds;Parent=MsG0880047306.01.T02
Chr8	CDS	84393744	84394075	84393744	ID=MsG0880047306.01.T02:cds;Parent=MsG0880047306.01.T02
Chr8	CDS	84393264	84393492	84393264	ID=MsG0880047306.01.T02:cds;Parent=MsG0880047306.01.T02
Chr8	three_prime_UTR	84393230	84393263	84393230	ID=MsG0880047306.01.T02:three_prime_utr;Parent=MsG0880047306.01.T02
Chr8	three_prime_UTR	84391775	84392644	84391775	ID=MsG0880047306.01.T02:three_prime_utr;Parent=MsG0880047306.01.T02

Gene Sequence

>MsG0880047306.01.T02

ATGGACCAGCGCCTGATATATGCATCCAATCAAGGTAAAATTACTCTTTCAGAAGATTCAAAGAACTCCTATCAGAAAGTGAAATCAATTGGTTCTAAGGAAAAGGTTGTGGAGACAGATGTGGTCACTAGGTCTGCAAGAAGCCATAGCAGTTCTGATTCCTTTCTTTTGGAAACAAAACCTGATGGAAACCTTGATTTAGAAAACTCTAGCCACCTTAGCCAGTCTGGTGAAATGATCAAAGGTGAGGACAAGAGTAAAGACACAGGCCAGAGTAAATTTGATCTTTCTCTCTCTCATGGCACAAATGTCAATGATAAATTAAACTCTCCGACATGGACTGGGGAGAACCACCCGGAAAATACACTATACGAAGAAAATGGTTGTCCAGCTCGTGATGTAGCAGCTGTGACAGTTCATTCACCTACGGTAATAGCTACTGCAAAATCAAATCTTGATAATTATACTGCTGGTATAGGTGGAACCGAAACAGGATACACTCATGACTCTAAATTGCATTCCAAAGGTCTTGACACTAGATGGTCTGGGATGCCTTTTGCTAACTCATGTTCATTCAACAAAACTTCTGCCTTCAATACACAGAAACTTGTTGATTACAACTTGTCCACATTTTATCATTACGAGAGTTGGACCGCCAGACGGGGTTCTTTTAATGATGACAGTTCCCTTGGAAAAGTGAAATATAATGTAACTTGCTACTATGCAAAGAGATTTGAGGCTCTAAGAAGAACTTGTTGCCCTTCTGAGCTTGATTTTGTGCGGTCCCTTAGTCGTTGTAAGAAGTGGGGGGCTCAGGGAGGAAAGAGCAATGTTTTCTTTGCAAAAACCTTGGATGATAGATTTATCGTCAAGCAGGTTACAAAGACAGAGCTTGAATCATTATCAAGTTTGCACCGGCTTATTTTAAATATTTATCAGAGTCGATCAGTACAGGAAGCCCAACTTGTCTTGCAAAGATCTTGGGAATTTATCAGGTACATCAAAGCACCTCAAAGGAGGGAAGGAAACAAAAATGGATGTTTTGGTTATGGAAAATCTTCTTATCGGCGCAACATAAGACGGCTTTATGATCTCAAAGGTTCTTCTCGATCAAGGTACAATCCAGATACAAGTGGGAGCAATAAAGTTCTGCTGGATCAGAATCTCATTGAAGCTATGCCACCTCTCCAATATTTGTTGGAACAAGGCAAAGCGGCTTCTTGA

>MsG0880047306.01.T01

Protein sequence

>MsG0880047306.01.T02

MDQRLIYASNQGKITLSEDSKNSYQKVKSIGSKEKVVETDVVTRSARSHSSSDSFLLETKPDGNLDLENSSHLSQSGEMIKGEDKSKDTGQSKFDLSLSHGTNVNDKLNSPTWTGENHPENTLYEENGCPARDVAAVTVHSPTVIATAKSNLDNYTAGIGGTETGYTHDSKLHSKGLDTRWSGMPFANSCSFNKTSAFNTQKLVDYNLSTFYHYESWTARRGSFNDDSSLGKVKYNVTCYYAKRFEALRRTCCPSELDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIVKQVTKTELESLSSLHRLILNIYQSRSVQEAQLVLQRSWEFIRYIKAPQRREGNKNGCFGYGKSSYRRNIRRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPPLQYLLEQGKAAS*

>MsG0880047306.01.T01