AlfalfaGEDB | MsG0180004017.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004017.01

KEGG: -
GO: GO:0005525 GO:0006508 GO:0008236
PFAM: PF01926 PF03572

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004017.01.T01	XP_024636143.1	89.313	262	27	1	264	524	218	479	8.91E-151	451
MsG0180004017.01.T01	XP_024636143.1	96.721	61	2	0	211	271	99	159	2.20E-26	124

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004017.01.T01	F4HSD4	55.586	367	110	6	211	527	130	493	2.56E-117	358

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004017.01.T01	G7I2N7	89.313	262	27	1	264	524	248	509	2.36e-150	449

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047883.01	MsG0180004017.01	0.807562	4.757610e-50	2.658368e-47
MsG0080047884.01	MsG0180004017.01	0.813170	2.931032e-51	1.900052e-48
MsG0080047885.01	MsG0180004017.01	0.813307	2.734282e-51	1.779264e-48
MsG0080048506.01	MsG0180004017.01	-0.812521	4.066534e-51	2.591288e-48
MsG0080048507.01	MsG0180004017.01	-0.810787	9.691203e-51	5.896472e-48
MsG0080048528.01	MsG0180004017.01	0.819127	1.367265e-52	1.043082e-49
MsG0080048660.01	MsG0180004017.01	0.807260	5.513990e-50	3.057051e-47
MsG0080048727.01	MsG0180004017.01	0.824119	9.591359e-54	8.418550e-51
MsG0080048748.01	MsG0180004017.01	0.802250	6.133495e-49	2.990167e-46
MsG0080048773.01	MsG0180004017.01	0.829083	6.277603e-55	6.357906e-52

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004017.01.T01	MTR_1g073010	89.313	262	27	1	264	524	248	509	5.98e-154	449
MsG0180004017.01.T01	MTR_1g073010	96.721	61	2	0	211	271	129	189	3.73e-30	124
MsG0180004017.01.T01	MTR_1g073130	82.692	104	18	0	6	109	114	217	1.16e-49	178
MsG0180004017.01.T01	MTR_1g073130	96.875	64	2	0	117	180	284	347	5.19e-32	129
MsG0180004017.01.T01	MTR_1g073130	91.667	36	3	0	182	217	432	467	3.00e-12	69.3
MsG0180004017.01.T01	MTR_2g019790	45.506	178	87	3	329	503	308	478	3.23e-36	142
MsG0180004017.01.T01	MTR_1g023160	37.963	216	113	4	277	484	213	415	2.87e-26	112

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004017.01.T01	AT1G07615	55.586	367	110	6	211	527	130	493	2.61e-118	358
MsG0180004017.01.T01	AT4G17740	76.000	100	24	0	6	105	116	215	5.11e-45	166
MsG0180004017.01.T01	AT4G17740	76.562	64	15	0	117	180	286	349	2.97e-25	109
MsG0180004017.01.T01	AT4G17740	88.889	36	4	0	182	217	434	469	1.84e-11	67.0
MsG0180004017.01.T01	AT4G17740	88.095	84	10	0	6	89	126	209	7.08e-45	166
MsG0180004017.01.T01	AT4G17740	76.562	64	15	0	117	180	296	359	3.26e-25	109
MsG0180004017.01.T01	AT4G17740	88.889	36	4	0	182	217	444	479	2.05e-11	66.6
MsG0180004017.01.T01	AT5G18570	44.382	178	89	3	329	503	368	538	2.97e-35	140

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Find 132 sgRNAs with CRISPR-Local

Find 653 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGACATGCCATCCGA+TGG	0.615475	1:-71917291	MsG0180004017.01.T01:CDS
AACAGATTATTGTTGCGTGG+AGG	0.662217	1:-71909549	MsG0180004017.01.T01:CDS
AAGAAAAGGAATTTCACCGT+GGG	0.658650	1:-71909076	MsG0180004017.01.T01:CDS
AAGAAAGATGCTTGCCACAT+TGG	0.503483	1:-71917519	MsG0180004017.01.T01:CDS
AAGAAGCAATCAATACGTTC+AGG	0.477783	1:-71915043	MsG0180004017.01.T01:CDS
AAGACAACAAGTCCATCGGA+TGG	0.675511	1:+71917279	None:intergenic
AAGCCTCCGACTACGAAGGC+AGG	0.456383	1:-71909483	MsG0180004017.01.T01:CDS
AAGGCATAGTGACCAACAGC+AGG	0.566091	1:+71909282	None:intergenic
AAGGGTGCACACCAAAATCG+TGG	0.658610	1:-71909180	MsG0180004017.01.T01:CDS
AAGTCATACATGTCTCTTCA+CGG	0.503051	1:+71918578	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAATAAGGTATGACTGG+AGG	+	Chr1:71912946-71912965	None:intergenic	30.0%
AAAAATAAGATAGGAACCAG+GGG	-	Chr1:71917459-71917478	MsG0180004017.01.T01:intron	30.0%
AAACGCAAACCCTTAAAATT+TGG	-	Chr1:71916063-71916082	MsG0180004017.01.T01:intron	30.0%
AAATAAATAAGACAGCTGCA+AGG	+	Chr1:71912046-71912065	None:intergenic	30.0%
AAATATAATGTAGGTACGGT+CGG	-	Chr1:71915136-71915155	MsG0180004017.01.T01:intron	30.0%
AAGATGAATCAATGCATTTC+AGG	-	Chr1:71911493-71911512	MsG0180004017.01.T01:intron	30.0%
AATAAATAAGACAGCTGCAA+GGG	+	Chr1:71912045-71912064	None:intergenic	30.0%
AATCATGCGATATTTGATCT+AGG	+	Chr1:71918728-71918747	None:intergenic	30.0%
AATGTGCTTGTTTAATGTAG+TGG	-	Chr1:71917243-71917262	MsG0180004017.01.T01:intron	30.0%
AATTCTTGTCGATATATGCT+CGG	+	Chr1:71908878-71908897	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	71908810	71918749	71908810	ID=MsG0180004017.01;Name=MsG0180004017.01
Chr1	mRNA	71908810	71918749	71908810	ID=MsG0180004017.01.T01;Parent=MsG0180004017.01;Name=MsG0180004017.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|0.42\|0.5\|1\|0.85\|1\|8\|0\|527
Chr1	exon	71918587	71918749	71918587	ID=MsG0180004017.01.T01:exon:31035;Parent=MsG0180004017.01.T01
Chr1	exon	71917469	71917551	71917469	ID=MsG0180004017.01.T01:exon:31034;Parent=MsG0180004017.01.T01
Chr1	exon	71917263	71917363	71917263	ID=MsG0180004017.01.T01:exon:31033;Parent=MsG0180004017.01.T01
Chr1	exon	71915926	71916044	71915926	ID=MsG0180004017.01.T01:exon:31032;Parent=MsG0180004017.01.T01
Chr1	exon	71914999	71915074	71914999	ID=MsG0180004017.01.T01:exon:31031;Parent=MsG0180004017.01.T01
Chr1	exon	71913288	71913381	71913288	ID=MsG0180004017.01.T01:exon:31030;Parent=MsG0180004017.01.T01
Chr1	exon	71909774	71909950	71909774	ID=MsG0180004017.01.T01:exon:31029;Parent=MsG0180004017.01.T01
Chr1	exon	71908810	71909580	71908810	ID=MsG0180004017.01.T01:exon:31028;Parent=MsG0180004017.01.T01
Chr1	CDS	71918587	71918749	71918587	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71917469	71917551	71917469	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71917263	71917363	71917263	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71915926	71916044	71915926	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71914999	71915074	71914999	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71913288	71913381	71913288	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71909774	71909950	71909774	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01
Chr1	CDS	71908810	71909580	71908810	ID=MsG0180004017.01.T01:cds;Parent=MsG0180004017.01.T01

Gene Sequence

>MsG0180004017.01.T01

TTGTCCCTTGAAGGGGCCCTGACGGAAGAGAATCTCCTCTTTCTGGAGGCGTGGAGAACAATTGACCGAGCATATATCGACAAGAATTTTAATGGACAGAGTTGGTTTAGGTATAGAGAAAATGCATTGCGCAACGAACCCATGAACAACCGTGAAGAGACATATATGGCGATAAGAAAGATGCTTGCCACATTGGATGATCCCTTCACTAGATTTTTAGAGCCTGAGAAGTTCAGAAGTTTGAGGTCTGGAACTAAAGGCGCTCTACTGGAGTGGGATATCATGGCTACCCTACCAAAGCAGACATGCCATCCGATGGACTTGTTGTCTTTCAGCTTCTCCAGGAGGCGAGAGAAAGTTACAGTCAATCCAGTGAAGTCAAGACTGTGCAAGTTACCTGCTGCAGGAAATAATTCCCCAACAGTTGGTTATATAAAATTAACATCGTTCAACCAAAATGCATCTCGTGCTATCAAAGAAGCAATCAATACGTTCAGGAGTAATAATGTAAATGCATTTGTTTTGGACCTCAGAGATAATAGGAAGATCCAATCAGTATTTGAGCTCTCAGATGGCTCAGGACTAGTTGTTACAGTTGCTCGCTACGAGACACCTGCGCACACAGATATTGACAAGGTTGTTCGTGTACCGATTGGCACTGTACTTCATCTTGTTAGTGGTGATATTCCTTCTATAGTAAAAAAAGTTCAATCCTCGGCAGATTTAGACCCTTGGGATGTTCCTGGTGCACTTGTAGATGATCATTCCGACCCTCGCGATGGTTCTACTTCCAACGTTACAAAGCAGGAAAAAAAGAAGGTCAACAGATTATTGTTGCGTGGAGGAGAAGGTGGTCTCGGTAATGTGTCTATTCAAAAAGATCCTAGGAAGCCTCCGACTACGAAGGCAGGGGTAGGTCAACACGTAACCAATGTTCAGGACACTGACAGTGTTAACTTCCTTAAGTACAGGTTTACCTGGTCTGAAACTGTTCTTATATTAGAATTGAAGTGCATTGCTGATGTTAGTTTTGTGGGAATGCCTAATGCTGGTAAAAGCACTTTACTTGGAGCTATATCGAGAGCTAAGCCTGCTGTTGGTCACTATGCCTTCACTACCCTTAGGCCGAATTTAGGGAATTTGAACTATGATGATATGTCGATAATTGTGGCTGATATTCCTGGACTTATAAAGGGTGCACACCAAAATCGTGGACTTGGACATGCATTTCTGCGCCACATAGAACGCACGAAGGTTCTGGCTTATGTGGTAGACGTGGCTGCTGCTTTACCGGGAAGAAAAGGAATTTCACCGTGGGAACAACTGAAAGACTTGGTTCTAGAGCTTGAGTACCATCTGGAGGGTTTATCCAATCGGCCATCCTTGATAGTCGCAAATAAGATTGATGAGGAAGGTGCTGAGGAAGTGTATGAAGAGTTAAAGAGAAGAGTCCAGGGTGTTCCTATCTTTGCTGTGAGTGCTGTTTTGGAGGAAGGGATACCAGAGCTTAAAGCTGGTCTTAGAGTGCTTGTCAATGGTGAAACTTCATGCCCACTTTACCTAGATCAAATATCGCATGATTAG

Protein sequence

>MsG0180004017.01.T01

LSLEGALTEENLLFLEAWRTIDRAYIDKNFNGQSWFRYRENALRNEPMNNREETYMAIRKMLATLDDPFTRFLEPEKFRSLRSGTKGALLEWDIMATLPKQTCHPMDLLSFSFSRRREKVTVNPVKSRLCKLPAAGNNSPTVGYIKLTSFNQNASRAIKEAINTFRSNNVNAFVLDLRDNRKIQSVFELSDGSGLVVTVARYETPAHTDIDKVVRVPIGTVLHLVSGDIPSIVKKVQSSADLDPWDVPGALVDDHSDPRDGSTSNVTKQEKKKVNRLLLRGGEGGLGNVSIQKDPRKPPTTKAGVGQHVTNVQDTDSVNFLKYRFTWSETVLILELKCIADVSFVGMPNAGKSTLLGAISRAKPAVGHYAFTTLRPNLGNLNYDDMSIIVADIPGLIKGAHQNRGLGHAFLRHIERTKVLAYVVDVAAALPGRKGISPWEQLKDLVLELEYHLEGLSNRPSLIVANKIDEEGAEEVYEELKRRVQGVPIFAVSAVLEEGIPELKAGLRVLVNGETSCPLYLDQISHD*