Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004201.01.T01 | XP_013468806.1 | 90.948 | 696 | 25 | 1 | 1 | 658 | 12 | 707 | 0 | 1298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004201.01.T01 | A0A072VN49 | 90.948 | 696 | 25 | 1 | 1 | 658 | 12 | 707 | 0.0 | 1298 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004201.01 | MsG0180004325.01 | 0.974983 | 3.980255e-139 | 5.794190e-133 |
| MsG0180004201.01 | MsG0280007073.01 | 0.803196 | 3.912393e-49 | 1.953899e-46 |
| MsG0180004201.01 | MsG0280011446.01 | 0.802012 | 6.864655e-49 | 3.326304e-46 |
| MsG0180004201.01 | MsG0380016264.01 | 0.825680 | 4.108083e-54 | 3.770329e-51 |
| MsG0180004201.01 | MsG0480019816.01 | 0.812141 | 4.922934e-51 | 3.105127e-48 |
| MsG0180004201.01 | MsG0480022274.01 | 0.815854 | 7.466556e-52 | 5.206614e-49 |
| MsG0180004201.01 | MsG0480022480.01 | 0.801042 | 1.084940e-48 | 5.130147e-46 |
| MsG0180004201.01 | MsG0780040143.01 | 0.818044 | 2.406391e-52 | 1.781751e-49 |
| MsG0180004201.01 | MsG0780040442.01 | 0.855645 | 5.490746e-62 | 1.276445e-58 |
| MsG0180004201.01 | MsG0780040713.01 | 0.828745 | 7.577115e-55 | 7.598189e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004201.01.T01 | MTR_1g076990 | 90.948 | 696 | 25 | 1 | 1 | 658 | 12 | 707 | 0.0 | 1298 |
| MsG0180004201.01.T01 | MTR_1g076990 | 96.029 | 554 | 22 | 0 | 105 | 658 | 12 | 565 | 0.0 | 1108 |
| MsG0180004201.01.T01 | MTR_1g076990 | 95.851 | 482 | 20 | 0 | 177 | 658 | 56 | 537 | 0.0 | 956 |
| MsG0180004201.01.T01 | MTR_4g035550 | 47.170 | 689 | 304 | 11 | 1 | 638 | 11 | 690 | 0.0 | 610 |
| MsG0180004201.01.T01 | MTR_5g077750 | 30.114 | 704 | 376 | 20 | 2 | 623 | 11 | 680 | 5.74e-85 | 281 |
| MsG0180004201.01.T01 | MTR_5g040700 | 29.393 | 643 | 386 | 18 | 26 | 619 | 33 | 656 | 1.60e-80 | 268 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004201.01.T01 | AT1G33420 | 47.577 | 681 | 293 | 17 | 4 | 644 | 18 | 674 | 0.0 | 607 |
| MsG0180004201.01.T01 | AT1G33420 | 49.372 | 557 | 252 | 12 | 95 | 644 | 36 | 569 | 0.0 | 532 |
| MsG0180004201.01.T01 | AT1G66170 | 32.724 | 602 | 333 | 19 | 72 | 618 | 69 | 653 | 9.84e-80 | 267 |
| MsG0180004201.01.T01 | AT5G22260 | 29.264 | 598 | 366 | 15 | 75 | 625 | 78 | 665 | 1.52e-79 | 266 |
| MsG0180004201.01.T01 | AT2G01810 | 28.529 | 666 | 379 | 20 | 28 | 618 | 39 | 682 | 2.17e-74 | 253 |
| MsG0180004201.01.T01 | AT2G07714 | 34.746 | 118 | 74 | 1 | 165 | 279 | 1 | 118 | 1.48e-20 | 90.5 |
Find 190 sgRNAs with CRISPR-Local
Find 414 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTGGAGTGTATGAATTC+AGG | 0.437264 | 1:+74798756 | MsG0180004201.01.T01:CDS |
| AAAGAAATCGTGGTAATCGG+CGG | 0.688894 | 1:-74795092 | None:intergenic |
| AAAGAGTGACGTTGAACGTG+TGG | 0.580901 | 1:+74798559 | MsG0180004201.01.T01:CDS |
| AAATAACAGATAGGCATTGG+TGG | 0.591327 | 1:+74800532 | MsG0180004201.01.T01:three_prime_UTR |
| AAATCCATGGTATGGTGAGT+GGG | 0.494538 | 1:+74798235 | MsG0180004201.01.T01:CDS |
| AAGAACTCTTGCAATGAGCT+CGG | 0.581985 | 1:+74799789 | MsG0180004201.01.T01:CDS |
| AAGAAGGTGCCCATAACCAT+TGG | 0.553682 | 1:-74797633 | None:intergenic |
| AAGCCATGCTCAGAGTGCTG+CGG | 0.591353 | 1:+74798585 | MsG0180004201.01.T01:CDS |
| AAGCGGCGAGGGATGGTGAA+GGG | 0.618092 | 1:-74795120 | None:intergenic |
| AAGCGGCGGATTGATGCTTC+TGG | 0.330006 | 1:+74798518 | MsG0180004201.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTTTAACTGCTTAAAAGG+AGG | + | Chr1:74798825-74798844 | MsG0180004201.01.T01:intron | 30.0% | |
| AATGGAGAAAAATACTCACT+TGG | - | Chr1:74796726-74796745 | None:intergenic | 30.0% | |
| AATTTAGACAAATCTGACAC+TGG | - | Chr1:74799871-74799890 | None:intergenic | 30.0% | |
| ACAACTAAGCAAAATCCAAA+TGG | - | Chr1:74796049-74796068 | None:intergenic | 30.0% | |
| AGGATGACAATTGCATAAAA+TGG | - | Chr1:74796744-74796763 | None:intergenic | 30.0% | |
| AGTACATTACACTTAGGATT+TGG | + | Chr1:74798017-74798036 | MsG0180004201.01.T01:intron | 30.0% | |
| ATAGCCTGTAAATAACAGAT+AGG | + | Chr1:74800523-74800542 | MsG0180004201.01.T01:three_prime_UTR | 30.0% | |
| ATGGATGTTTCGAAGAAATA+CGG | + | Chr1:74798176-74798195 | MsG0180004201.01.T01:CDS | 30.0% | |
| CGATAAAATGGAGAGATATT+AGG | + | Chr1:74795770-74795789 | MsG0180004201.01.T01:intron | 30.0% | |
| CTAATACTTCTGTTAAGTAG+TGG | + | Chr1:74797832-74797851 | MsG0180004201.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 74795076 | 74800647 | 74795076 | ID=MsG0180004201.01;Name=MsG0180004201.01 |
| Chr1 | mRNA | 74795076 | 74800647 | 74795076 | ID=MsG0180004201.01.T01;Parent=MsG0180004201.01;Name=MsG0180004201.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0.66|0.75|1|1|1|4|177|658 |
| Chr1 | exon | 74795076 | 74795392 | 74795076 | ID=MsG0180004201.01.T01:exon:31646;Parent=MsG0180004201.01.T01 |
| Chr1 | exon | 74797532 | 74797747 | 74797532 | ID=MsG0180004201.01.T01:exon:31647;Parent=MsG0180004201.01.T01 |
| Chr1 | exon | 74798163 | 74798777 | 74798163 | ID=MsG0180004201.01.T01:exon:31648;Parent=MsG0180004201.01.T01 |
| Chr1 | exon | 74799642 | 74800647 | 74799642 | ID=MsG0180004201.01.T01:exon:31649;Parent=MsG0180004201.01.T01 |
| Chr1 | CDS | 74795076 | 74795392 | 74795076 | ID=MsG0180004201.01.T01:cds;Parent=MsG0180004201.01.T01 |
| Chr1 | CDS | 74797532 | 74797747 | 74797532 | ID=MsG0180004201.01.T01:cds;Parent=MsG0180004201.01.T01 |
| Chr1 | CDS | 74798163 | 74798777 | 74798163 | ID=MsG0180004201.01.T01:cds;Parent=MsG0180004201.01.T01 |
| Chr1 | CDS | 74799642 | 74800470 | 74799642 | ID=MsG0180004201.01.T01:cds;Parent=MsG0180004201.01.T01 |
| Chr1 | three_prime_UTR | 74800471 | 74800647 | 74800471 | ID=MsG0180004201.01.T01:three_prime_utr;Parent=MsG0180004201.01.T01 |
| Gene Sequence |
>MsG0180004201.01.T01 ATGAAAAGAAGAGTCACCGCCGATTACCACGATTTCTTTTCATTCCCTTCACCATCCCTCGCCGCTTCAGAGAGTTTCTCCGGCGGACCTTTTCGTTCTAATGTTCACTCTTTTCTCACCAAGTACGCGCTTCTTCCACCGCCTTCCGCGTTGTTTCCGCACTTGCTGACGTGGCAGATCCTCTTCCGTGTCGGTGAGATCACCGATGGGGGTGAATCGGGACCGGCGGTTATTTGCCTTGACGTTGTGGAAGAAGATGTTGCTAGATCCAGATCTGTGTATTGTGATCAGTGCCGAGTTTTTGGTGAGTTGACTCGGTGCAAGTCATGTAACCATGTGACAACCAGTGATGATGTTGAAGACTGGGTGTACCACCAACTAGAGAACACAAGTCATCTTTTACATGGTGTAGTCCATGCCAATGGTTATGGGCACCTTCTTCGAGTTAATGGCAGAGAAGGGGGATCTAGATATCTTTCTGGTTGCCATATTATGGATTTCTGGGATCGCCTATGCAAGACACTTGGAGCTAGAAAAGTTAGTGTGATGGATGTTTCGAAGAAATACGGGCTAGAGTATCGCTTGCTCCATGCCATTATGAAGGGAAATCCATGGTATGGTGAGTGGGGATATAATTTTGGCTCTGGCAGCTATTGTCTCACACAGGAAGCATACAAGTCTGCTGTTGAAAGCCTATCCAATCTACCCTTGTCCATCTTCCTCTCCCAGGAACAGAAGCCTCACTCACGTGTGCAGGATATGATTTTGTATTACCGGTCTTTGTCAGAGCATGAGCTTGTAAATATGAGGGACCTATTTTGTTTTTTGATGGGTTTGTTGCATGATGCTCGCAAGACAGCCTCTAAGTCTGATGACATAACCTGCAAAAAGCGGCGGATTGATGCTTCTGGATTATCAAGTTCTTGGGAAAAGAGTGACGTTGAACGTGTGGAGGAAGCCATGCTCAGAGTGCTGCGGGCAGTGTCTGGGTCTAATTGGGTGAGTGGTCGAGCTCTTAAGGGTGCTGTTTGCAAGGTGGCCTCTCCAGAGCTTCTTGATTATTGCCTTTCTGAACTGGGAGGAAAAGTAGTCTATGGTGGGATTGTTAATAGCAGATGCAATCCTCAAACTGGAGTGTATGAATTCAGGATTGATGCTACAAATGTTTTTATGGCCAACAATAATTCTCCAGGCTCAAAGCGTCCTTCTGAAGAGAATATCCTGCAATGCTTGAGATATTTATACGACTCTCTGCTTCATCCTTGGATGATGCTAAACTATGTTGATGAAGAGACAAGAACTCTTGCAATGAGCTCGGCTCAGAAGCTTCTTGACTGCAAGCAATTAGTAAAAGATTATTGCTCTGAGATGCCGCCAGTGTCAGATTTGTCTAAATTACGTATCTCATGTCAGGTTGAACTAGTTGACCAATCTGAAGATACTGAAGCCAAAATTCCACCAGAAATAATTGTGCTGCCCATGAACGCCACAGTGTCTGACCTAAAGATTGAAGCAGCAAAAGCTTTTCAGGAAGTATATCTAATGTTTAGAAAATTTCAAGTTGACGAACTAGTTGGCTACAGCGGTGTAGAAGATTCCACCCAGGTCAAGTTCTTGTTAGGATCAAATGAAGTGGTTTGTGTCCGAGGAAAATACTCCGGGAAGAATGGGTTGAGCAAGTTTCGAATGGAACGTGGACTTGAGAGGTGGACTGTCGAGTGCAACTGTGGGGCTAAGGATGATGATGGAGAGAGAATGATGGCTTGTGATATGTGTGGTGTGTGGCGGCACACTAGGTGTTTTGGCATTCCTGATACTGCTCCTGTTCCAGCCCGTTTTGTTTGTTCCAGATGCCAAAACTGTGACTCAAAGCTTAAATCAGGTGGCCACTGTAAAGATGAGAGACTGGCTAGTGTTACCACAACTAGTGGTAGTTACATGGGAAACAGCTTTTCAGTGCCTTCTAATATTCGTTAA |
| Protein sequence |
>MsG0180004201.01.T01 MKRRVTADYHDFFSFPSPSLAASESFSGGPFRSNVHSFLTKYALLPPPSALFPHLLTWQILFRVGEITDGGESGPAVICLDVVEEDVARSRSVYCDQCRVFGELTRCKSCNHVTTSDDVEDWVYHQLENTSHLLHGVVHANGYGHLLRVNGREGGSRYLSGCHIMDFWDRLCKTLGARKVSVMDVSKKYGLEYRLLHAIMKGNPWYGEWGYNFGSGSYCLTQEAYKSAVESLSNLPLSIFLSQEQKPHSRVQDMILYYRSLSEHELVNMRDLFCFLMGLLHDARKTASKSDDITCKKRRIDASGLSSSWEKSDVERVEEAMLRVLRAVSGSNWVSGRALKGAVCKVASPELLDYCLSELGGKVVYGGIVNSRCNPQTGVYEFRIDATNVFMANNNSPGSKRPSEENILQCLRYLYDSLLHPWMMLNYVDEETRTLAMSSAQKLLDCKQLVKDYCSEMPPVSDLSKLRISCQVELVDQSEDTEAKIPPEIIVLPMNATVSDLKIEAAKAFQEVYLMFRKFQVDELVGYSGVEDSTQVKFLLGSNEVVCVRGKYSGKNGLSKFRMERGLERWTVECNCGAKDDDGERMMACDMCGVWRHTRCFGIPDTAPVPARFVCSRCQNCDSKLKSGGHCKDERLASVTTTSGSYMGNSFSVPSNIR* |
