Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004447.01.T01 | GAU21858.1 | 78.313 | 83 | 4 | 1 | 2 | 84 | 129 | 197 | 1.54E-36 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004447.01.T01 | Q5QIT3 | 54.762 | 84 | 23 | 2 | 2 | 84 | 127 | 196 | 1.42E-23 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004447.01.T01 | A0A2Z6LV68 | 78.313 | 83 | 4 | 1 | 2 | 84 | 129 | 197 | 7.36e-37 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000164.01 | MsG0180004447.01 | 0.840763 | 7.147747e-58 | 1.028468e-54 |
| MsG0180004447.01 | MsG0180006034.01 | 0.818584 | 1.816258e-52 | 1.364977e-49 |
| MsG0180004447.01 | MsG0280010884.01 | 0.829930 | 3.906262e-55 | 4.055746e-52 |
| MsG0180004447.01 | MsG0380016547.01 | 0.840165 | 1.024826e-57 | 1.447632e-54 |
| MsG0180004447.01 | MsG0380017776.01 | 0.847926 | 8.490196e-60 | 1.532711e-56 |
| MsG0180004447.01 | MsG0480019162.01 | 0.819731 | 9.954510e-53 | 7.723372e-50 |
| MsG0180004447.01 | MsG0480023973.01 | 0.845073 | 5.097868e-59 | 8.396925e-56 |
| MsG0180004447.01 | MsG0580024837.01 | 0.804016 | 2.644603e-49 | 1.348894e-46 |
| MsG0180004447.01 | MsG0780036184.01 | 0.829980 | 3.800037e-55 | 3.951025e-52 |
| MsG0180004447.01 | MsG0780041470.01 | 0.875085 | 4.069381e-68 | 1.876129e-64 |
| MsG0180004447.01 | MsG0880045704.01 | 0.845036 | 5.214992e-59 | 8.580053e-56 |
| MsG0180004447.01 | MsG0880046825.01 | 0.824798 | 6.639191e-54 | 5.942638e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004447.01.T01 | MTR_1g080030 | 79.518 | 83 | 3 | 1 | 2 | 84 | 130 | 198 | 5.60e-38 | 132 |
| MsG0180004447.01.T01 | MTR_7g116150 | 65.060 | 83 | 15 | 1 | 2 | 84 | 132 | 200 | 3.57e-31 | 114 |
| MsG0180004447.01.T01 | MTR_1g075800 | 48.810 | 84 | 28 | 2 | 2 | 84 | 131 | 200 | 1.65e-20 | 84.0 |
| MsG0180004447.01.T01 | MTR_1g075800 | 48.810 | 84 | 28 | 2 | 2 | 84 | 102 | 171 | 1.73e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004447.01.T01 | AT5G28850 | 54.762 | 84 | 23 | 2 | 2 | 84 | 127 | 196 | 1.45e-24 | 95.5 |
| MsG0180004447.01.T01 | AT5G28900 | 54.762 | 84 | 23 | 2 | 2 | 84 | 127 | 196 | 1.46e-24 | 95.5 |
| MsG0180004447.01.T01 | AT5G44090 | 57.143 | 84 | 21 | 2 | 2 | 84 | 165 | 234 | 2.39e-24 | 95.1 |
| MsG0180004447.01.T01 | AT5G44090 | 57.143 | 84 | 21 | 2 | 2 | 84 | 130 | 199 | 2.92e-24 | 94.7 |
| MsG0180004447.01.T01 | AT1G03960 | 53.571 | 84 | 24 | 2 | 2 | 84 | 121 | 190 | 7.03e-22 | 88.2 |
| MsG0180004447.01.T01 | AT1G03960 | 53.571 | 84 | 24 | 2 | 2 | 84 | 121 | 190 | 7.52e-22 | 87.8 |
| MsG0180004447.01.T01 | AT1G03960 | 53.571 | 84 | 24 | 2 | 2 | 84 | 59 | 128 | 7.75e-22 | 87.8 |
| MsG0180004447.01.T01 | AT1G54450 | 50.000 | 84 | 27 | 2 | 2 | 84 | 127 | 196 | 3.18e-21 | 86.3 |
Find 9 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGTGATCACGAAATAAC+TGG | 0.419598 | 1:+78138794 | None:intergenic |
| CCAGTTATTTCGTGATCACT+TGG | 0.429206 | 1:-78138794 | MsG0180004447.01.T01:CDS |
| AGAATTCAAATCAATTACTA+AGG | 0.497111 | 1:-78138462 | MsG0180004447.01.T01:CDS |
| TGATCACTTGGATGGACTAC+AGG | 0.514009 | 1:-78138782 | MsG0180004447.01.T01:CDS |
| TTATTTCGTGATCACTTGGA+TGG | 0.515402 | 1:-78138790 | MsG0180004447.01.T01:CDS |
| GCGGAGCCGGTGTTGTGACA+AGG | 0.600640 | 1:-78138385 | MsG0180004447.01.T01:CDS |
| TTGGATGGACTACAGGTGCA+TGG | 0.652281 | 1:-78138775 | MsG0180004447.01.T01:intron |
| CACTCACCTTGTCACAACAC+CGG | 0.680206 | 1:+78138379 | None:intergenic |
| GTGATCACGAAATAACTGGT+CGG | 0.706593 | 1:+78138798 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGTTTTAATTGTTTTCT+TGG | - | Chr1:78138756-78138775 | MsG0180004447.01.T01:intron | 15.0% |
| !! | AGAATTCAAATCAATTACTA+AGG | - | Chr1:78138785-78138804 | MsG0180004447.01.T01:CDS | 20.0% |
| ! | GAAAAGAGAAGTTGAAAAAA+AGG | + | Chr1:78138829-78138848 | None:intergenic | 25.0% |
| !! | AATTTTTGCTCAACTTAAAG+AGG | + | Chr1:78138645-78138664 | None:intergenic | 25.0% |
| !! | ATTTTTGCTCAACTTAAAGA+GGG | + | Chr1:78138644-78138663 | None:intergenic | 25.0% |
| !! | CATTGTTCTTTTAGATCATT+TGG | + | Chr1:78138418-78138437 | None:intergenic | 25.0% |
| AGAGAAGTTGAAAAAAAGGA+TGG | + | Chr1:78138825-78138844 | None:intergenic | 30.0% | |
| ATTTAGCTGTACTCAAAAAG+TGG | - | Chr1:78138676-78138695 | MsG0180004447.01.T01:intron | 30.0% | |
| ! | CTTTTCAGAAAAATTGACAG+CGG | - | Chr1:78138843-78138862 | MsG0180004447.01.T01:CDS | 30.0% |
| ! | TGTTCTTTTAGATCATTTGG+AGG | + | Chr1:78138415-78138434 | None:intergenic | 30.0% |
| ! | TGTTTAAAATCATCTTTCCC+AGG | + | Chr1:78138551-78138570 | None:intergenic | 30.0% |
| ! | TTTTTGCTCAACTTAAAGAG+GGG | + | Chr1:78138643-78138662 | None:intergenic | 30.0% |
| TTATTTCGTGATCACTTGGA+TGG | - | Chr1:78138457-78138476 | MsG0180004447.01.T01:CDS | 35.0% | |
| ! | AGTTTTTCTTTCAACACGGA+CGG | - | Chr1:78138386-78138405 | MsG0180004447.01.T01:CDS | 35.0% |
| ! | CTGCAGTTTTTCTTTCAACA+CGG | - | Chr1:78138382-78138401 | MsG0180004447.01.T01:CDS | 35.0% |
| ! | TCTTTTAGATCATTTGGAGG+TGG | + | Chr1:78138412-78138431 | None:intergenic | 35.0% |
| CCAAGTGATCACGAAATAAC+TGG | + | Chr1:78138456-78138475 | None:intergenic | 40.0% | |
| GTGATCACGAAATAACTGGT+CGG | + | Chr1:78138452-78138471 | None:intergenic | 40.0% | |
| ! | CCAGTTATTTCGTGATCACT+TGG | - | Chr1:78138453-78138472 | MsG0180004447.01.T01:CDS | 40.0% |
| AGAAAAATTGACAGCGGAGC+CGG | - | Chr1:78138849-78138868 | MsG0180004447.01.T01:CDS | 45.0% | |
| TGCTCAACTTAAAGAGGGGA+AGG | + | Chr1:78138639-78138658 | None:intergenic | 45.0% | |
| TTTCCCAGGTTGCAACAAAC+TGG | + | Chr1:78138537-78138556 | None:intergenic | 45.0% | |
| !! | TGATCACTTGGATGGACTAC+AGG | - | Chr1:78138465-78138484 | MsG0180004447.01.T01:CDS | 45.0% |
| GGCTCTGCAGGCTATAAACT+AGG | + | Chr1:78138516-78138535 | None:intergenic | 50.0% | |
| !! | AGAGCCAGTTTGTTGCAACC+TGG | - | Chr1:78138530-78138549 | MsG0180004447.01.T01:intron | 50.0% |
| !! | GAGCCAGTTTGTTGCAACCT+GGG | - | Chr1:78138531-78138550 | MsG0180004447.01.T01:intron | 50.0% |
| !! | TTGCAACAAACTGGCTCTGC+AGG | + | Chr1:78138528-78138547 | None:intergenic | 50.0% |
| !! | TTGGATGGACTACAGGTGCA+TGG | - | Chr1:78138472-78138491 | MsG0180004447.01.T01:CDS | 50.0% |
| !! | GCGGAGCCGGTGTTGTGACA+AGG | - | Chr1:78138862-78138881 | MsG0180004447.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78138382 | 78138887 | 78138382 | ID=MsG0180004447.01;Name=MsG0180004447.01 |
| Chr1 | mRNA | 78138382 | 78138887 | 78138382 | ID=MsG0180004447.01.T01;Parent=MsG0180004447.01;Name=MsG0180004447.01.T01;_AED=0.48;_eAED=0.55;_QI=0|0|0|1|0|0|2|0|84 |
| Chr1 | exon | 78138776 | 78138887 | 78138776 | ID=MsG0180004447.01.T01:exon:45321;Parent=MsG0180004447.01.T01 |
| Chr1 | exon | 78138382 | 78138524 | 78138382 | ID=MsG0180004447.01.T01:exon:45320;Parent=MsG0180004447.01.T01 |
| Chr1 | CDS | 78138776 | 78138887 | 78138776 | ID=MsG0180004447.01.T01:cds;Parent=MsG0180004447.01.T01 |
| Chr1 | CDS | 78138382 | 78138524 | 78138382 | ID=MsG0180004447.01.T01:cds;Parent=MsG0180004447.01.T01 |
| Gene Sequence |
| Protein sequence |
