Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019162.01.T01 | XP_013447253.1 | 95.21 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 2.95E-110 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019162.01.T01 | Q9C723 | 76.647 | 167 | 39 | 0 | 1 | 167 | 1 | 167 | 2.87E-96 | 296 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019162.01.T01 | A0A072U616 | 95.210 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 1.41e-110 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000164.01 | MsG0480019162.01 | 0.800860 | 1.182270e-48 | 5.564349e-46 |
| MsG0180000318.01 | MsG0480019162.01 | 0.830652 | 2.602888e-55 | 2.759581e-52 |
| MsG0180001706.01 | MsG0480019162.01 | 0.832643 | 8.407128e-56 | 9.454437e-53 |
| MsG0180002264.01 | MsG0480019162.01 | 0.824485 | 7.869197e-54 | 6.979483e-51 |
| MsG0180004335.01 | MsG0480019162.01 | 0.829055 | 6.375217e-55 | 6.451175e-52 |
| MsG0180004447.01 | MsG0480019162.01 | 0.819731 | 9.954510e-53 | 7.723372e-50 |
| MsG0280010884.01 | MsG0480019162.01 | 0.856736 | 2.630448e-62 | 6.338819e-59 |
| MsG0380013794.01 | MsG0480019162.01 | 0.831997 | 1.215094e-55 | 1.340688e-52 |
| MsG0480019161.01 | MsG0480019162.01 | 0.977058 | 5.002003e-143 | 8.719228e-137 |
| MsG0480019162.01 | MsG0480019163.01 | 0.921479 | 3.393369e-88 | 1.216915e-83 |
| MsG0480019162.01 | MsG0480021490.01 | 0.842526 | 2.450038e-58 | 3.725248e-55 |
| MsG0480019162.01 | MsG0480021845.01 | 0.806511 | 7.939736e-50 | 4.317475e-47 |
| MsG0480019162.01 | MsG0480022939.01 | 0.814515 | 1.481568e-51 | 9.962256e-49 |
| MsG0480019162.01 | MsG0480023726.01 | -0.804636 | 1.964645e-49 | 1.018121e-46 |
| MsG0480019162.01 | MsG0480023973.01 | 0.885816 | 5.864741e-72 | 4.085849e-68 |
| MsG0480019162.01 | MsG0780036184.01 | 0.814599 | 1.419214e-51 | 9.564402e-49 |
| MsG0480019162.01 | MsG0780041470.01 | 0.838379 | 2.978366e-57 | 3.981961e-54 |
| MsG0480019162.01 | MsG0880043027.01 | 0.819774 | 9.732784e-53 | 7.560705e-50 |
| MsG0480019162.01 | MsG0880044225.01 | 0.809881 | 1.519546e-50 | 9.026977e-48 |
| MsG0480019162.01 | MsG0880045605.01 | 0.803275 | 3.767194e-49 | 1.885178e-46 |
| MsG0480019162.01 | MsG0880045704.01 | 0.813527 | 2.446403e-51 | 1.601548e-48 |
| MsG0480019162.01 | MsG0880046825.01 | 0.821292 | 4.363519e-53 | 3.535492e-50 |
| MsG0480019162.01 | MsG0880047179.01 | 0.854567 | 1.129716e-61 | 2.533756e-58 |
| MsG0380016828.01 | MsG0480019162.01 | 0.800974 | 1.120225e-48 | 5.287835e-46 |
| MsG0380017776.01 | MsG0480019162.01 | 0.829807 | 4.186314e-55 | 4.331755e-52 |
| MsG0280007111.01 | MsG0480019162.01 | 0.821680 | 3.550216e-53 | 2.907996e-50 |
| MsG0280009344.01 | MsG0480019162.01 | 0.809913 | 1.496021e-50 | 8.894559e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019162.01.T01 | MTR_8g099885 | 95.210 | 167 | 8 | 0 | 1 | 167 | 1 | 167 | 3.57e-114 | 340 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019162.01.T01 | AT1G55090 | 76.647 | 167 | 39 | 0 | 1 | 167 | 1 | 167 | 2.92e-97 | 296 |
Find 42 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTTATTACGAATGGTTAA+TGG | 0.329703 | 4:+15238154 | None:intergenic |
| GGAATGTTTGAAAGATATAT+TGG | 0.334800 | 4:-15238049 | MsG0480019162.01.T01:CDS |
| GGTTAATGGTGTCGAGTTCT+AGG | 0.340380 | 4:+15238168 | None:intergenic |
| GTCTTTGATTTGTTGAAGAT+TGG | 0.361625 | 4:+15238277 | None:intergenic |
| ATCATTTGCAAGCCACATCT+TGG | 0.378781 | 4:+15237912 | None:intergenic |
| CTCTCAGAACCTTTGATAAT+AGG | 0.381396 | 4:+15237985 | None:intergenic |
| AAGATATATTGGTTGGTGAT+TGG | 0.409668 | 4:-15238038 | MsG0480019162.01.T01:CDS |
| ATAACCGGGGATTTCGAGTT+CGG | 0.410395 | 4:+15238208 | None:intergenic |
| GTGGTCTTCACAGACATAAC+CGG | 0.410799 | 4:+15238193 | None:intergenic |
| AACTTCGATGGTTTACTGCT+TGG | 0.416004 | 4:-15237876 | MsG0480019162.01.T01:CDS |
| GTCGTATCATAATAATCTTT+CGG | 0.425212 | 4:+15237935 | None:intergenic |
| TCCTAATGTTTCCGAGGCAT+TGG | 0.428289 | 4:-15237818 | MsG0480019162.01.T01:CDS |
| GCCGGCGTCGTCGTCCGTCT+TGG | 0.458553 | 4:-15238233 | MsG0480019162.01.T01:CDS |
| CAATCTTAACCAATGGGCTA+TGG | 0.462534 | 4:-15238309 | MsG0480019162.01.T01:CDS |
| GATAACATAGTGTGCAGTAT+TGG | 0.463634 | 4:-15238012 | MsG0480019162.01.T01:CDS |
| GGCTTGTTTAGATTTGTAGA+TGG | 0.463676 | 4:+15238253 | None:intergenic |
| TTCTGAGAGATACAATTGTC+AGG | 0.465019 | 4:-15237971 | MsG0480019162.01.T01:CDS |
| TCATTTGCAAGCCACATCTT+GGG | 0.476765 | 4:+15237913 | None:intergenic |
| GCCAATGCCTCGGAAACATT+AGG | 0.480001 | 4:+15237817 | None:intergenic |
| TAACCGGGGATTTCGAGTTC+GGG | 0.480511 | 4:+15238209 | None:intergenic |
| TACTTGCAATCTTAACCAAT+GGG | 0.505809 | 4:-15238315 | MsG0480019162.01.T01:CDS |
| AATCACGATGAGGCTCTTGA+AGG | 0.512546 | 4:-15238342 | None:intergenic |
| GTTTACTGCTTGGAAACAAA+GGG | 0.526848 | 4:-15237866 | MsG0480019162.01.T01:CDS |
| TGGTGTCGAGTTCTAGGAAG+TGG | 0.535115 | 4:+15238174 | None:intergenic |
| TTAACAAAACCATAACCAAA+AGG | 0.535794 | 4:+15237781 | None:intergenic |
| CTACTTGCAATCTTAACCAA+TGG | 0.542643 | 4:-15238316 | MsG0480019162.01.T01:CDS |
| ATTGGAATGCCTATTATCAA+AGG | 0.545628 | 4:-15237994 | MsG0480019162.01.T01:CDS |
| AATCGAATTCCATAGCCCAT+TGG | 0.548373 | 4:+15238300 | None:intergenic |
| GGTTTACTGCTTGGAAACAA+AGG | 0.548451 | 4:-15237867 | MsG0480019162.01.T01:CDS |
| ACCAAGACGGACGACGACGC+CGG | 0.556438 | 4:+15238232 | None:intergenic |
| GAAGTTGAAAATCATCAAGT+TGG | 0.562415 | 4:+15237842 | None:intergenic |
| TGGTCTTCACAGACATAACC+GGG | 0.571329 | 4:+15238194 | None:intergenic |
| GTAATTATAGAGAACTTCGA+TGG | 0.573265 | 4:-15237888 | MsG0480019162.01.T01:CDS |
| ATCTACAAATCTAAACAAGC+CGG | 0.574227 | 4:-15238251 | MsG0480019162.01.T01:CDS |
| TGTTTGAAAGATATATTGGT+TGG | 0.589747 | 4:-15238045 | MsG0480019162.01.T01:CDS |
| AAGATGTGGCTTGCAAATGA+TGG | 0.599672 | 4:-15237910 | MsG0480019162.01.T01:CDS |
| ACTTCCTCCTAATGTTTCCG+AGG | 0.602942 | 4:-15237824 | MsG0480019162.01.T01:CDS |
| TTATGATACGACCCAAGATG+TGG | 0.618671 | 4:-15237924 | MsG0480019162.01.T01:CDS |
| GGTCCCGAACTCGAAATCCC+CGG | 0.647848 | 4:-15238212 | MsG0480019162.01.T01:CDS |
| AATGCCTCGGAAACATTAGG+AGG | 0.652440 | 4:+15237820 | None:intergenic |
| TTTCGAGTTCGGGACCAAGA+CGG | 0.655937 | 4:+15238219 | None:intergenic |
| GGTCTTCACAGACATAACCG+GGG | 0.797753 | 4:+15238195 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAAAGAGTTATTACGAA+TGG | + | Chr4:15237956-15237975 | None:intergenic | 25.0% |
| ! | GGAATGTTTGAAAGATATAT+TGG | - | Chr4:15238051-15238070 | MsG0480019162.01.T01:CDS | 25.0% |
| ! | GTCGTATCATAATAATCTTT+CGG | + | Chr4:15238168-15238187 | None:intergenic | 25.0% |
| ! | TGTTTGAAAGATATATTGGT+TGG | - | Chr4:15238055-15238074 | MsG0480019162.01.T01:CDS | 25.0% |
| ! | TTAACAAAACCATAACCAAA+AGG | + | Chr4:15238322-15238341 | None:intergenic | 25.0% |
| !!! | TTTGTTTTTGTCACTGAAAT+TGG | - | Chr4:15238011-15238030 | MsG0480019162.01.T01:CDS | 25.0% |
| ATCTACAAATCTAAACAAGC+CGG | - | Chr4:15237849-15237868 | MsG0480019162.01.T01:CDS | 30.0% | |
| ATTGGAATGCCTATTATCAA+AGG | - | Chr4:15238106-15238125 | MsG0480019162.01.T01:intron | 30.0% | |
| GAAGTTGAAAATCATCAAGT+TGG | + | Chr4:15238261-15238280 | None:intergenic | 30.0% | |
| GAGTTATTACGAATGGTTAA+TGG | + | Chr4:15237949-15237968 | None:intergenic | 30.0% | |
| GTAATTATAGAGAACTTCGA+TGG | - | Chr4:15238212-15238231 | MsG0480019162.01.T01:CDS | 30.0% | |
| TACTTGCAATCTTAACCAAT+GGG | - | Chr4:15237785-15237804 | MsG0480019162.01.T01:CDS | 30.0% | |
| !! | AAATCAGTTCCTTTTGGTTA+TGG | - | Chr4:15238310-15238329 | MsG0480019162.01.T01:CDS | 30.0% |
| !! | AAGATATATTGGTTGGTGAT+TGG | - | Chr4:15238062-15238081 | MsG0480019162.01.T01:intron | 30.0% |
| !! | GTCTTTGATTTGTTGAAGAT+TGG | + | Chr4:15237826-15237845 | None:intergenic | 30.0% |
| CTACTTGCAATCTTAACCAA+TGG | - | Chr4:15237784-15237803 | MsG0480019162.01.T01:CDS | 35.0% | |
| GATAACATAGTGTGCAGTAT+TGG | - | Chr4:15238088-15238107 | MsG0480019162.01.T01:intron | 35.0% | |
| GTTTACTGCTTGGAAACAAA+GGG | - | Chr4:15238234-15238253 | MsG0480019162.01.T01:CDS | 35.0% | |
| ! | CTCTCAGAACCTTTGATAAT+AGG | + | Chr4:15238118-15238137 | None:intergenic | 35.0% |
| ! | GCACAAAAATCAGTTCCTTT+TGG | - | Chr4:15238304-15238323 | MsG0480019162.01.T01:CDS | 35.0% |
| ! | GGCTTGTTTAGATTTGTAGA+TGG | + | Chr4:15237850-15237869 | None:intergenic | 35.0% |
| ! | TTCTGAGAGATACAATTGTC+AGG | - | Chr4:15238129-15238148 | MsG0480019162.01.T01:intron | 35.0% |
| AACTTCGATGGTTTACTGCT+TGG | - | Chr4:15238224-15238243 | MsG0480019162.01.T01:CDS | 40.0% | |
| AATCGAATTCCATAGCCCAT+TGG | + | Chr4:15237803-15237822 | None:intergenic | 40.0% | |
| ATCATTTGCAAGCCACATCT+TGG | + | Chr4:15238191-15238210 | None:intergenic | 40.0% | |
| GGTTTACTGCTTGGAAACAA+AGG | - | Chr4:15238233-15238252 | MsG0480019162.01.T01:CDS | 40.0% | |
| TCATTTGCAAGCCACATCTT+GGG | + | Chr4:15238190-15238209 | None:intergenic | 40.0% | |
| TTATGATACGACCCAAGATG+TGG | - | Chr4:15238176-15238195 | MsG0480019162.01.T01:CDS | 40.0% | |
| ! | AAGATGTGGCTTGCAAATGA+TGG | - | Chr4:15238190-15238209 | MsG0480019162.01.T01:CDS | 40.0% |
| ! | TGATTTTTGTGCCAATGCCT+CGG | + | Chr4:15238296-15238315 | None:intergenic | 40.0% |
| !! | CAATCTTAACCAATGGGCTA+TGG | - | Chr4:15237791-15237810 | MsG0480019162.01.T01:CDS | 40.0% |
| !!! | ATTGGTTTTGTAGCTGCAGA+TGG | - | Chr4:15238029-15238048 | MsG0480019162.01.T01:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCTGCAGAT+GGG | - | Chr4:15238030-15238049 | MsG0480019162.01.T01:CDS | 40.0% |
| AATGCCTCGGAAACATTAGG+AGG | + | Chr4:15238283-15238302 | None:intergenic | 45.0% | |
| ACTTCCTCCTAATGTTTCCG+AGG | - | Chr4:15238276-15238295 | MsG0480019162.01.T01:CDS | 45.0% | |
| GTGGTCTTCACAGACATAAC+CGG | + | Chr4:15237910-15237929 | None:intergenic | 45.0% | |
| TCCTAATGTTTCCGAGGCAT+TGG | - | Chr4:15238282-15238301 | MsG0480019162.01.T01:CDS | 45.0% | |
| TGGTCTTCACAGACATAACC+GGG | + | Chr4:15237909-15237928 | None:intergenic | 45.0% | |
| ! | GGTTAATGGTGTCGAGTTCT+AGG | + | Chr4:15237935-15237954 | None:intergenic | 45.0% |
| !! | ATAACCGGGGATTTCGAGTT+CGG | + | Chr4:15237895-15237914 | None:intergenic | 45.0% |
| GCCAATGCCTCGGAAACATT+AGG | + | Chr4:15238286-15238305 | None:intergenic | 50.0% | |
| GGTCTTCACAGACATAACCG+GGG | + | Chr4:15237908-15237927 | None:intergenic | 50.0% | |
| !! | TAACCGGGGATTTCGAGTTC+GGG | + | Chr4:15237894-15237913 | None:intergenic | 50.0% |
| !! | TGGTGTCGAGTTCTAGGAAG+TGG | + | Chr4:15237929-15237948 | None:intergenic | 50.0% |
| !! | TTTCGAGTTCGGGACCAAGA+CGG | + | Chr4:15237884-15237903 | None:intergenic | 50.0% |
| GGTCCCGAACTCGAAATCCC+CGG | - | Chr4:15237888-15237907 | MsG0480019162.01.T01:CDS | 60.0% | |
| ACCAAGACGGACGACGACGC+CGG | + | Chr4:15237871-15237890 | None:intergenic | 65.0% | |
| GCCGGCGTCGTCGTCCGTCT+TGG | - | Chr4:15237867-15237886 | MsG0480019162.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 15237765 | 15238357 | 15237765 | ID=MsG0480019162.01;Name=MsG0480019162.01 |
| Chr4 | mRNA | 15237765 | 15238357 | 15237765 | ID=MsG0480019162.01.T01;Parent=MsG0480019162.01;Name=MsG0480019162.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|167 |
| Chr4 | exon | 15238164 | 15238357 | 15238164 | ID=MsG0480019162.01.T01:exon:3428;Parent=MsG0480019162.01.T01 |
| Chr4 | exon | 15237765 | 15238074 | 15237765 | ID=MsG0480019162.01.T01:exon:3427;Parent=MsG0480019162.01.T01 |
| Chr4 | CDS | 15238164 | 15238357 | 15238164 | ID=MsG0480019162.01.T01:cds;Parent=MsG0480019162.01.T01 |
| Chr4 | CDS | 15237765 | 15238074 | 15237765 | ID=MsG0480019162.01.T01:cds;Parent=MsG0480019162.01.T01 |
| Gene Sequence |
| Protein sequence |
