AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000762.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000762.01.T01 MTR_1g009030 88.961 154 2 1 1 154 79 217 1.33e-94 273
MsG0180000762.01.T01 MTR_3g109280 81.818 154 26 1 1 154 79 230 3.73e-90 262
MsG0180000762.01.T01 MTR_1g077210 72.327 159 39 2 1 154 79 237 1.44e-77 230
MsG0180000762.01.T01 MTR_0054s0080 70.130 154 46 0 1 154 79 232 2.99e-75 224
MsG0180000762.01.T01 MTR_6g066040 58.000 150 61 1 1 150 79 226 1.53e-56 177
MsG0180000762.01.T01 MTR_6g066090 53.896 154 68 1 1 154 6 156 3.51e-55 171
MsG0180000762.01.T01 MTR_7g073370 41.441 111 62 1 27 137 104 211 4.01e-21 85.9
MsG0180000762.01.T01 MTR_5g068640 31.210 157 101 2 1 151 77 232 9.51e-21 85.5
MsG0180000762.01.T01 MTR_2g014820 38.235 136 76 3 1 134 722 851 4.09e-16 74.7
MsG0180000762.01.T01 MTR_8g106520 34.074 135 84 2 1 135 21 150 1.01e-15 70.9
MsG0180000762.01.T01 MTR_8g106520 34.074 135 84 2 1 135 77 206 3.53e-15 70.5
MsG0180000762.01.T01 MTR_8g106530 36.170 94 60 0 42 135 114 207 3.61e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000762.01.T01 AT4G31330 71.069 159 39 2 1 153 79 236 1.21e-78 233
MsG0180000762.01.T01 AT5G10580 70.186 161 39 2 1 152 79 239 1.97e-75 225
MsG0180000762.01.T01 AT5G10580 71.338 157 36 2 1 148 79 235 1.10e-74 223
MsG0180000762.01.T01 AT5G10580 71.338 157 36 2 1 148 79 235 1.10e-74 223
MsG0180000762.01.T01 AT5G10580 71.028 107 23 1 1 99 79 185 1.31e-49 158
MsG0180000762.01.T01 AT5G24790 59.477 153 56 3 1 147 79 231 3.33e-49 159
MsG0180000762.01.T01 AT3G18215 33.333 135 90 0 1 135 6 140 1.36e-18 78.2
MsG0180000762.01.T01 AT3G18215 33.333 135 90 0 1 135 78 212 8.57e-18 77.4
MsG0180000762.01.T01 AT5G24600 27.439 164 105 2 1 152 77 238 2.36e-16 73.9
MsG0180000762.01.T01 AT5G24600 28.571 147 91 2 1 135 77 221 1.84e-14 68.6
MsG0180000762.01.T01 AT5G43180 33.766 154 87 3 2 140 79 232 3.62e-12 62.4

Find 23 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAATATATATTTGAAATTT+TGG 0.097168 1:+10774668 MsG0180000762.01.T01:CDS
CTGGACTTCCTTTGTTGCTT+TGG 0.242448 1:+10774736 MsG0180000762.01.T01:CDS
CCACTAAATGATGCAATATA+TGG 0.301604 1:+10774492 MsG0180000762.01.T01:CDS
CCATATATTGCATCATTTAG+TGG 0.330574 1:-10774492 None:intergenic
GAGCAAAGGAAAACCATAAC+AGG 0.393179 1:-10774768 None:intergenic
AAAATTTCAAATATATATTC+TGG 0.418167 1:-10774666 None:intergenic
TTTCCTTTGCTCTCTTGCTA+TGG 0.429928 1:+10774779 MsG0180000762.01.T01:CDS
TAAATCTTATTGCAAGAGAA+TGG 0.439278 1:-10774587 None:intergenic
AAAGATGAATGTGGATCAAA+AGG 0.472647 1:+10774848 MsG0180000762.01.T01:CDS
TTTGTGTACACTAGTGGAAA+GGG 0.482848 1:+10774825 MsG0180000762.01.T01:CDS
TGGAGCACATGGTGAATTCA+TGG 0.497913 1:+10774512 MsG0180000762.01.T01:CDS
TCTGGTGTCACAAGTGACAT+AGG 0.507387 1:-10774648 None:intergenic
ACATAGTAAAATTGAGGTTG+TGG 0.520784 1:-10774424 None:intergenic
AATGGCAAAAGAATGAGAAG+AGG 0.536492 1:-10774569 None:intergenic
ACCTGAACATAGTAAAATTG+AGG 0.548897 1:-10774430 None:intergenic
AAATCAAGATTGTAAAGCAC+AGG 0.577484 1:-10774804 None:intergenic
GATGAATGTGGATCAAAAGG+AGG 0.581198 1:+10774851 MsG0180000762.01.T01:CDS
GAACCATAGCAAGAGAGCAA+AGG 0.582292 1:-10774782 None:intergenic
CTGGTGTCACAAGTGACATA+GGG 0.583728 1:-10774647 None:intergenic
ATGAGAAGAGGAAAATTGTG+AGG 0.605718 1:-10774557 None:intergenic
ACAAGTGACATAGGGTCTTG+AGG 0.610415 1:-10774639 None:intergenic
TGGAAAGGGAAAGATGAATG+TGG 0.637565 1:+10774839 MsG0180000762.01.T01:CDS
GATGCAATATATGGAGCACA+TGG 0.696776 1:+10774501 MsG0180000762.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTCAAATATATATTC+TGG - Chr1:10774669-10774688 None:intergenic 10.0%
!!! AGAATATATATTTGAAATTT+TGG + Chr1:10774668-10774687 MsG0180000762.01.T01:CDS 10.0%
!!! ATATTTGAAATTTTGGAGAA+AGG + Chr1:10774675-10774694 MsG0180000762.01.T01:CDS 20.0%
! TAAATCTTATTGCAAGAGAA+TGG - Chr1:10774590-10774609 None:intergenic 25.0%
AAAGATGAATGTGGATCAAA+AGG + Chr1:10774848-10774867 MsG0180000762.01.T01:CDS 30.0%
AAATCAAGATTGTAAAGCAC+AGG - Chr1:10774807-10774826 None:intergenic 30.0%
ACATAGTAAAATTGAGGTTG+TGG - Chr1:10774427-10774446 None:intergenic 30.0%
ACCTGAACATAGTAAAATTG+AGG - Chr1:10774433-10774452 None:intergenic 30.0%
CCACTAAATGATGCAATATA+TGG + Chr1:10774492-10774511 MsG0180000762.01.T01:CDS 30.0%
CCATATATTGCATCATTTAG+TGG - Chr1:10774495-10774514 None:intergenic 30.0%
! ACCTCAATTTTACTATGTTC+AGG + Chr1:10774429-10774448 MsG0180000762.01.T01:CDS 30.0%
! TTTTGAATGCTGTTGGAAAT+AGG + Chr1:10774703-10774722 MsG0180000762.01.T01:CDS 30.0%
AATGGCAAAAGAATGAGAAG+AGG - Chr1:10774572-10774591 None:intergenic 35.0%
ATGAGAAGAGGAAAATTGTG+AGG - Chr1:10774560-10774579 None:intergenic 35.0%
CCAAAAATCCAAAGCAACAA+AGG - Chr1:10774747-10774766 None:intergenic 35.0%
TTTGTGTACACTAGTGGAAA+GGG + Chr1:10774825-10774844 MsG0180000762.01.T01:CDS 35.0%
! CTTGATTTTGTGTACACTAG+TGG + Chr1:10774819-10774838 MsG0180000762.01.T01:CDS 35.0%
! GGTTTCATTTTGAATGCTGT+TGG + Chr1:10774696-10774715 MsG0180000762.01.T01:CDS 35.0%
! TTTTGTGTACACTAGTGGAA+AGG + Chr1:10774824-10774843 MsG0180000762.01.T01:CDS 35.0%
!!! CCTTTGTTGCTTTGGATTTT+TGG + Chr1:10774744-10774763 MsG0180000762.01.T01:CDS 35.0%
!!! TTGGATTTTTGGTCCTGTTA+TGG + Chr1:10774755-10774774 MsG0180000762.01.T01:CDS 35.0%
GAGCAAAGGAAAACCATAAC+AGG - Chr1:10774771-10774790 None:intergenic 40.0%
GATGAATGTGGATCAAAAGG+AGG + Chr1:10774851-10774870 MsG0180000762.01.T01:CDS 40.0%
GATGCAATATATGGAGCACA+TGG + Chr1:10774501-10774520 MsG0180000762.01.T01:CDS 40.0%
TGGAAAGGGAAAGATGAATG+TGG + Chr1:10774839-10774858 MsG0180000762.01.T01:CDS 40.0%
TTTCCTTTGCTCTCTTGCTA+TGG + Chr1:10774779-10774798 MsG0180000762.01.T01:CDS 40.0%
! GGAAATAGGCTTTTCTATGC+TGG + Chr1:10774717-10774736 MsG0180000762.01.T01:CDS 40.0%
ACAAGTGACATAGGGTCTTG+AGG - Chr1:10774642-10774661 None:intergenic 45.0%
GAACCATAGCAAGAGAGCAA+AGG - Chr1:10774785-10774804 None:intergenic 45.0%
TGGAGCACATGGTGAATTCA+TGG + Chr1:10774512-10774531 MsG0180000762.01.T01:CDS 45.0%
! CTGGACTTCCTTTGTTGCTT+TGG + Chr1:10774736-10774755 MsG0180000762.01.T01:CDS 45.0%
!! CTGGTGTCACAAGTGACATA+GGG - Chr1:10774650-10774669 None:intergenic 45.0%
!! TCTGGTGTCACAAGTGACAT+AGG - Chr1:10774651-10774670 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 10774420 10774884 10774420 ID=MsG0180000762.01;Name=MsG0180000762.01
Chr1 mRNA 10774420 10774884 10774420 ID=MsG0180000762.01.T01;Parent=MsG0180000762.01;Name=MsG0180000762.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|154
Chr1 exon 10774420 10774884 10774420 ID=MsG0180000762.01.T01:exon:26009;Parent=MsG0180000762.01.T01
Chr1 CDS 10774420 10774884 10774420 ID=MsG0180000762.01.T01:cds;Parent=MsG0180000762.01.T01
Gene Sequence

>MsG0180000762.01.T01

ATGGCCACAACCTCAATTTTACTATGTTCAGGTCTAGCAGCAGTTATAAGCAGCACATATAGTGTGAAGAAACCACTAAATGATGCAATATATGGAGCACATGGTGAATTCATGGTTGCACTAAAATACGTGTCACTCCTCACAATTTTCCTCTTCTCATTCTTTTGCCATTCTCTTGCAATAAGATTTATTAATCAAGTGAACATTTTAATCAATACTCCTCAAGACCCTATGTCACTTGTGACACCAGAATATATATTTGAAATTTTGGAGAAAGGTTTCATTTTGAATGCTGTTGGAAATAGGCTTTTCTATGCTGGACTTCCTTTGTTGCTTTGGATTTTTGGTCCTGTTATGGTTTTCCTTTGCTCTCTTGCTATGGTTCCTGTGCTTTACAATCTTGATTTTGTGTACACTAGTGGAAAGGGAAAGATGAATGTGGATCAAAAGGAGGATTATGTTTGA

Protein sequence

>MsG0180000762.01.T01

MATTSILLCSGLAAVISSTYSVKKPLNDAIYGAHGEFMVALKYVSLLTIFLFSFFCHSLAIRFINQVNILINTPQDPMSLVTPEYIFEILEKGFILNAVGNRLFYAGLPLLLWIFGPVMVFLCSLAMVPVLYNLDFVYTSGKGKMNVDQKEDYV*