Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000440.01.T01 | XP_003588968.2 | 96.06 | 533 | 17 | 2 | 1 | 533 | 1 | 529 | 0 | 1046 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000440.01 | MsG0180003779.01 | -0.822718 | 2.038916e-53 | 1.720279e-50 |
| MsG0180000440.01 | MsG0180004491.01 | 0.822697 | 2.062199e-53 | 1.738872e-50 |
| MsG0180000440.01 | MsG0180004533.01 | 0.811926 | 5.481875e-51 | 3.437296e-48 |
| MsG0180000440.01 | MsG0180004777.01 | 0.805866 | 1.085285e-49 | 5.805478e-47 |
| MsG0180000440.01 | MsG0180005873.01 | 0.814588 | 1.427284e-51 | 9.616009e-49 |
| MsG0180000440.01 | MsG0280008492.01 | 0.805509 | 1.290187e-49 | 6.837897e-47 |
| MsG0180000440.01 | MsG0280008750.01 | 0.832284 | 1.031894e-55 | 1.147976e-52 |
| MsG0180000440.01 | MsG0380011693.01 | -0.835259 | 1.862185e-56 | 2.265165e-53 |
| MsG0180000440.01 | MsG0380012281.01 | -0.804136 | 2.496595e-49 | 1.277300e-46 |
| MsG0180000440.01 | MsG0380013100.01 | 0.801112 | 1.050039e-48 | 4.973650e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000440.01.T01 | MTR_1g015860 | 96.060 | 533 | 17 | 2 | 1 | 533 | 1 | 529 | 0.0 | 1046 |
| MsG0180000440.01.T01 | MTR_3g104560 | 79.278 | 526 | 102 | 3 | 11 | 533 | 16 | 537 | 0.0 | 866 |
| MsG0180000440.01.T01 | MTR_1g015860 | 95.991 | 424 | 13 | 2 | 1 | 424 | 1 | 420 | 0.0 | 820 |
| MsG0180000440.01.T01 | MTR_3g104560 | 78.164 | 403 | 84 | 2 | 134 | 533 | 1 | 402 | 0.0 | 658 |
| MsG0180000440.01.T01 | MTR_6g022450 | 26.276 | 529 | 319 | 17 | 31 | 530 | 19 | 505 | 2.16e-34 | 136 |
| MsG0180000440.01.T01 | MTR_0181s0030 | 23.810 | 483 | 321 | 12 | 39 | 509 | 33 | 480 | 6.57e-33 | 132 |
| MsG0180000440.01.T01 | MTR_8g012590 | 27.006 | 511 | 308 | 16 | 36 | 530 | 33 | 494 | 1.35e-32 | 131 |
| MsG0180000440.01.T01 | MTR_0171s0010 | 25.000 | 484 | 315 | 15 | 42 | 509 | 37 | 488 | 2.25e-31 | 127 |
| MsG0180000440.01.T01 | MTR_0171s0020 | 24.788 | 472 | 310 | 14 | 42 | 502 | 38 | 475 | 3.01e-31 | 127 |
| MsG0180000440.01.T01 | MTR_5g019010 | 24.847 | 491 | 306 | 14 | 42 | 514 | 36 | 481 | 7.98e-30 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000440.01.T01 | AT2G26170 | 71.937 | 506 | 126 | 3 | 27 | 532 | 32 | 521 | 0.0 | 730 |
| MsG0180000440.01.T01 | AT2G26170 | 71.930 | 456 | 108 | 4 | 79 | 532 | 1 | 438 | 0.0 | 648 |
| MsG0180000440.01.T01 | AT5G25140 | 27.105 | 487 | 307 | 17 | 42 | 518 | 31 | 479 | 1.33e-34 | 136 |
| MsG0180000440.01.T01 | AT3G26310 | 22.376 | 505 | 338 | 13 | 37 | 527 | 27 | 491 | 9.87e-32 | 128 |
| MsG0180000440.01.T01 | AT3G26300 | 24.779 | 452 | 287 | 13 | 39 | 478 | 30 | 440 | 3.54e-31 | 127 |
| MsG0180000440.01.T01 | AT5G25120 | 25.558 | 493 | 307 | 18 | 42 | 518 | 31 | 479 | 1.73e-30 | 125 |
| MsG0180000440.01.T01 | AT5G57260 | 23.188 | 483 | 310 | 16 | 38 | 502 | 28 | 467 | 1.92e-29 | 122 |
| MsG0180000440.01.T01 | AT4G13310 | 25.000 | 476 | 297 | 14 | 42 | 502 | 34 | 464 | 1.44e-28 | 119 |
| MsG0180000440.01.T01 | AT2G45560 | 23.866 | 507 | 307 | 19 | 42 | 520 | 40 | 495 | 7.97e-28 | 117 |
| MsG0180000440.01.T01 | AT5G24900 | 24.145 | 497 | 286 | 15 | 64 | 530 | 90 | 525 | 8.56e-28 | 117 |
Find 107 sgRNAs with CRISPR-Local
Find 213 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATGTGGTAGCCGAGC+CGG | 0.534170 | 1:-6054834 | None:intergenic |
| AAAATTGTAGGGACTTGCAA+TGG | 0.555262 | 1:+6054192 | MsG0180000440.01.T01:intron |
| AAACCATATTCAAGTTCTAC+AGG | 0.445626 | 1:-6055864 | None:intergenic |
| AAACCTGTAGAACTTGAATA+TGG | 0.313560 | 1:+6055861 | MsG0180000440.01.T01:CDS |
| AAATGGAGCAGATTTCATAT+GGG | 0.585010 | 1:+6053406 | MsG0180000440.01.T01:intron |
| AAATTGCTTCAAGAAATTGA+TGG | 0.490361 | 1:+6054908 | MsG0180000440.01.T01:CDS |
| AACAATATCATCAAGACGAC+CGG | 0.536041 | 1:-6054672 | None:intergenic |
| AACATCAGTTGCTAATCTTA+GGG | 0.456017 | 1:-6054357 | None:intergenic |
| AACCTAACCTGATCAAGATA+AGG | 0.522487 | 1:-6054983 | None:intergenic |
| AAGAGAATCGAAGAGTGTGT+CGG | 0.660631 | 1:+6054757 | MsG0180000440.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGGTTTATTCTTCACC+AGG | + | Chr1:6053548-6053567 | MsG0180000440.01.T01:CDS | 30.0% | |
| AAACCTGTAGAACTTGAATA+TGG | + | Chr1:6055861-6055880 | MsG0180000440.01.T01:CDS | 30.0% | |
| AAATGGAGCAGATTTCATAT+GGG | + | Chr1:6053406-6053425 | MsG0180000440.01.T01:intron | 30.0% | |
| AACATCAAAATACAAGGATG+CGG | - | Chr1:6054153-6054172 | None:intergenic | 30.0% | |
| AACATCAGTTGCTAATCTTA+GGG | - | Chr1:6054360-6054379 | None:intergenic | 30.0% | |
| AACCAGAGAACTAAAAGAAA+AGG | - | Chr1:6052969-6052988 | None:intergenic | 30.0% | |
| AAGTTCATCCTCATCAAAAA+GGG | - | Chr1:6053053-6053072 | None:intergenic | 30.0% | |
| ACTTTCCTTAACTTCCAAAA+TGG | - | Chr1:6052785-6052804 | None:intergenic | 30.0% | |
| AGATGACAATGTAAAAGATG+TGG | - | Chr1:6054849-6054868 | None:intergenic | 30.0% | |
| AGATTAGCAACTGATGTTAT+TGG | + | Chr1:6054362-6054381 | MsG0180000440.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 6052683 | 6055938 | 6052683 | ID=MsG0180000440.01;Name=MsG0180000440.01 |
| Chr1 | mRNA | 6052683 | 6055938 | 6052683 | ID=MsG0180000440.01.T01;Parent=MsG0180000440.01;Name=MsG0180000440.01.T01;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|5|0|533 |
| Chr1 | exon | 6052683 | 6052909 | 6052683 | ID=MsG0180000440.01.T01:exon:28028;Parent=MsG0180000440.01.T01 |
| Chr1 | exon | 6053417 | 6053569 | 6053417 | ID=MsG0180000440.01.T01:exon:28027;Parent=MsG0180000440.01.T01 |
| Chr1 | exon | 6054202 | 6054997 | 6054202 | ID=MsG0180000440.01.T01:exon:28026;Parent=MsG0180000440.01.T01 |
| Chr1 | exon | 6055103 | 6055198 | 6055103 | ID=MsG0180000440.01.T01:exon:28025;Parent=MsG0180000440.01.T01 |
| Chr1 | exon | 6055609 | 6055938 | 6055609 | ID=MsG0180000440.01.T01:exon:28024;Parent=MsG0180000440.01.T01 |
| Chr1 | CDS | 6052683 | 6052909 | 6052683 | ID=MsG0180000440.01.T01:cds;Parent=MsG0180000440.01.T01 |
| Chr1 | CDS | 6053417 | 6053569 | 6053417 | ID=MsG0180000440.01.T01:cds;Parent=MsG0180000440.01.T01 |
| Chr1 | CDS | 6054202 | 6054997 | 6054202 | ID=MsG0180000440.01.T01:cds;Parent=MsG0180000440.01.T01 |
| Chr1 | CDS | 6055103 | 6055198 | 6055103 | ID=MsG0180000440.01.T01:cds;Parent=MsG0180000440.01.T01 |
| Chr1 | CDS | 6055609 | 6055938 | 6055609 | ID=MsG0180000440.01.T01:cds;Parent=MsG0180000440.01.T01 |
| Gene Sequence |
>MsG0180000440.01.T01 ATGTTGTTTATTAGTGTAATCTCAAGTTATGTCCCTTTGGCTTCAACAATATTCATCTTAGTGGCTTTAATTGGAGGATTAGTGGGTTATCTCTATTGGCCATTTTGGAAGTTAAGGAAAGTTCCAGGCCCACCATCTCTGCCTCTTGTAGGACACCTTCCATTGCTAGCAAAGTATGGCCCTGACGTTTTCTCAGTCCTTGCCAAGCAATATGGCCCTATCTACAGATTTCATATGGGAAGACAACCTCTTATAATCATAGCAGATGCAGAGCTTTGTAAAGAAGTGGGAATCAAAAAATTCAAAGACATTCCAAACAGAAGTATTCCTTCTCCTATCTCTGCTTCTCCTCTTCACCAAAAAGGTTTATTCTTCACCAGGGACTTGCAATGGTCTACGATGAGAAATACTATATTATCGGTATACCAACCATCACACTTAGCCAACTTAGTGCCTAAAATGCAATCATTTATCGAATCAGCAATCCAAAATCTTAATGACACCTCAAAAGAAGACATAATCTTTTCAAATCTTTCCCTAAGATTAGCAACTGATGTTATTGGAGATGCAGCATTTGGTGTCAACTTTGGTCTCTCAAAGCCTCATTCAATTTGTGAATCAATGAACAATGTTAACAATGTAGAACAATCAAGTGCTAATAGTGATGATGTATCAAATTTCATCAATCAACACATTTACTCCACAACACAACTTAAGATGGATTTGTCTGGTTCCTTCTCAATTATAATTGGCCTAATTGCTCCTATTCTTCAAGAACCAATAAGGCAGATTTTGAAGAGAATACCAGGCACTATGGATTGGAAAATGGAATGTACAAATAAAAATTTAACCGGTCGTCTTGATGATATTGTTAAGAAGAGAATGGAAGATAAAAGCCGAACTTCAAAGAATTTCTTGTCGCTTATATTGAATGCAAGAGAATCGAAGAGTGTGTCGGAGAATGTCTTTTCATCTGATTACATAAGTGCTGTTACTTATGAACACTTACTTGCCGGCTCGGCTACCACATCTTTTACATTGTCATCTATTGTTTATTTGGTTGCTGGTCATCCTAATGTTGAGGAAAAATTGCTTCAAGAAATTGATGGGTTTGGTCCACATGATAAGATACCTAATGCTAAAGATCTCAATGAAAGTTTTCCTTATCTTGATCAGGTTATTAAAGAGGCAATGAGGATTTACACCGTCTCTCCGTTGGTTGCAAGAGAAACATCAAATGAAGTAGAAATAGGAGGTTACCTTCTTCCAAAGGGAACATGGGTATGGTTAGCACTTGGAGTTCTAGCAAAAGACTCAAGAAACTTTGCAGAGCCAGAAAAATTCAAACCGGAGAGGTTTGACCCAAAATGCGAAGAAATGAAACGAAGGCACCCTTATGCATTCATACCATTTGGAATTGGTCCAAGGGCATGTATTGGACAGAAATTTTCCTTGCAAGAGATCAAGCTTACTTTGATTCATTTATATAGAAAATATGTATTTCGTCATTCACTCAACATGGAAAAACCTGTAGAACTTGAATATGGTTTAGTTCTTAACTTCAAGCATGGTGTCAAGCTTAGAGTAATAAAAAGAACATAA |
| Protein sequence |
>MsG0180000440.01.T01 MLFISVISSYVPLASTIFILVALIGGLVGYLYWPFWKLRKVPGPPSLPLVGHLPLLAKYGPDVFSVLAKQYGPIYRFHMGRQPLIIIADAELCKEVGIKKFKDIPNRSIPSPISASPLHQKGLFFTRDLQWSTMRNTILSVYQPSHLANLVPKMQSFIESAIQNLNDTSKEDIIFSNLSLRLATDVIGDAAFGVNFGLSKPHSICESMNNVNNVEQSSANSDDVSNFINQHIYSTTQLKMDLSGSFSIIIGLIAPILQEPIRQILKRIPGTMDWKMECTNKNLTGRLDDIVKKRMEDKSRTSKNFLSLILNARESKSVSENVFSSDYISAVTYEHLLAGSATTSFTLSSIVYLVAGHPNVEEKLLQEIDGFGPHDKIPNAKDLNESFPYLDQVIKEAMRIYTVSPLVARETSNEVEIGGYLLPKGTWVWLALGVLAKDSRNFAEPEKFKPERFDPKCEEMKRRHPYAFIPFGIGPRACIGQKFSLQEIKLTLIHLYRKYVFRHSLNMEKPVELEYGLVLNFKHGVKLRVIKRT* |
