AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038861.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038861.01.T01 MTR_7g063520 94.902 255 10 2 1 253 1 254 7.58e-170 469
MsG0780038861.01.T01 MTR_5g080880 47.012 251 111 5 20 252 8 254 1.66e-67 212
MsG0780038861.01.T01 MTR_5g080870 38.565 223 117 6 31 252 16 219 1.59e-39 140
MsG0780038861.01.T01 MTR_4g006560 37.736 159 91 3 98 253 83 236 2.56e-28 108
MsG0780038861.01.T01 MTR_1g013120 30.736 231 142 9 28 253 16 233 2.43e-19 84.3
MsG0780038861.01.T01 MTR_7g091050 46.667 60 32 0 194 253 106 165 1.49e-12 64.7
MsG0780038861.01.T01 MTR_7g091050 46.667 60 32 0 194 253 141 200 1.85e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038861.01.T01 AT5G02050 47.601 271 120 6 1 253 1 267 3.33e-69 214
MsG0780038861.01.T01 AT3G55605 44.528 265 128 8 1 253 1 258 8.83e-62 195
MsG0780038861.01.T01 AT2G39795 42.292 253 136 6 4 253 5 250 1.33e-59 189
MsG0780038861.01.T01 AT5G05990 38.132 257 135 7 18 252 4 258 7.22e-47 157
MsG0780038861.01.T01 AT2G39790 33.597 253 153 8 1 252 1 239 1.90e-34 124
MsG0780038861.01.T01 AT1G15870 30.180 222 139 8 39 253 29 241 1.70e-27 106
MsG0780038861.01.T01 AT1G80720 30.808 198 126 5 58 253 53 241 3.30e-22 92.4
MsG0780038861.01.T01 AT1G80720 30.808 198 126 5 58 253 1 189 5.41e-22 90.5
MsG0780038861.01.T01 AT4G31930 31.535 241 142 9 19 253 11 234 2.11e-19 84.3
MsG0780038861.01.T01 AT4G32605 31.915 94 60 1 164 253 126 219 6.44e-11 60.8

Find 71 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTCATCCGGATTCTTTA+TGG 0.182617 7:-54243964 None:intergenic
ATGGAGTGTGTATGGAGTTT+GGG 0.281286 7:+54243901 MsG0780038861.01.T01:CDS
CTCTGTTGAGGTGCCACGTT+AGG 0.340860 7:-54243798 None:intergenic
AATGGAGTGTGTATGGAGTT+TGG 0.345306 7:+54243900 MsG0780038861.01.T01:CDS
GAGAGGGAGTGCGGCGGCGG+TGG 0.353608 7:-54241443 None:intergenic
CTATCATTCTCATTCTTCTC+GGG 0.360091 7:-54243837 None:intergenic
CAAAGACAACAAGGAATACT+TGG 0.378496 7:+54244206 MsG0780038861.01.T01:CDS
GCTATCATTCTCATTCTTCT+CGG 0.381925 7:-54243838 None:intergenic
CTATCAATCACAACTTCATC+AGG 0.386386 7:-54243936 None:intergenic
GATTGCGCTGTGGAAGGTTC+TGG 0.386945 7:-54241494 None:intergenic
CAAAGGAAATGGAGTGTGTA+TGG 0.388939 7:+54243893 MsG0780038861.01.T01:CDS
GAGGAGAGGGAGTGCGGCGG+CGG 0.393871 7:-54241446 None:intergenic
AGTTGAATAGTTCTTTCTCC+AGG 0.396432 7:-54243729 None:intergenic
TGAAGACCAGCTGGTATATG+AGG 0.426221 7:+54243986 MsG0780038861.01.T01:CDS
GTGCGGCGGCGGTGGCGCGA+CGG 0.481559 7:-54241435 None:intergenic
TTGAAAACAGTCACAACTAA+AGG 0.487624 7:-54243873 None:intergenic
AGCTGGTCTTCAGATTCATC+CGG 0.494031 7:-54243975 None:intergenic
TTTGAGATCGAGGACAACCC+TGG 0.494413 7:+54243711 MsG0780038861.01.T01:CDS
ACTTGAGAAAAGCCTTCTGC+AGG 0.495315 7:-54244122 None:intergenic
TAGCTTGTCCATAAAGAATC+CGG 0.497260 7:+54243956 MsG0780038861.01.T01:CDS
GGCGGTGGCGCGACGGAGAG+CGG 0.500941 7:-54241428 None:intergenic
TGGAGGAAGTTGCCGCTGCA+CGG 0.501833 7:-54241474 None:intergenic
TTGCCGCTGCACGGCGAGAG+AGG 0.509670 7:-54241465 None:intergenic
GGGTTGTCCTCGATCTCAAA+AGG 0.511746 7:-54243708 None:intergenic
CGAAGGAGAGTATCATCAGC+GGG 0.511851 7:-54241598 None:intergenic
TCTGTTGAGGTGCCACGTTA+GGG 0.512444 7:-54243797 None:intergenic
GTTGAATAGTTCTTTCTCCA+GGG 0.519495 7:-54243728 None:intergenic
AGAGAGGAGAGGGAGTGCGG+CGG 0.530798 7:-54241449 None:intergenic
GAGTGGTTTCATTGTGGCGA+AGG 0.535144 7:-54241528 None:intergenic
TGAAATCAACTGTGCTCTTG+AGG 0.536484 7:+54241633 MsG0780038861.01.T01:CDS
ACCTCAACAGAGTGAGGATG+AGG 0.540010 7:+54243809 MsG0780038861.01.T01:CDS
CTGCACGGCGAGAGAGGAGA+GGG 0.542154 7:-54241459 None:intergenic
ATTGTGGCGAAGGAGGTTAA+CGG 0.544318 7:-54241518 None:intergenic
CACAGTTGATTTCACTGTCA+AGG 0.547033 7:-54241624 None:intergenic
CCCTCTCGTAGCTTCGCTTA+CGG 0.550555 7:+54241559 MsG0780038861.01.T01:CDS
GCTCTTGAGGATAATAATGA+AGG 0.555442 7:+54241646 MsG0780038861.01.T01:CDS
TTGTGTTGGGTGTTATCCCT+CGG 0.555715 7:-54244155 None:intergenic
GCCTCATCCTCACTCTGTTG+AGG 0.559817 7:-54243810 None:intergenic
AAAGACAACAAGGAATACTT+GGG 0.563940 7:+54244207 MsG0780038861.01.T01:CDS
TGGTTTCATTGTGGCGAAGG+AGG 0.565255 7:-54241525 None:intergenic
AACGGCGATTGCGCTGTGGA+AGG 0.565623 7:-54241500 None:intergenic
GATCTCAAAAGGAAAATCAC+CGG 0.567645 7:-54243697 None:intergenic
GGATGAATCTGAAGACCAGC+TGG 0.570489 7:+54243977 MsG0780038861.01.T01:CDS
CAGGTCCCTCATATACCAGC+TGG 0.571273 7:-54243992 None:intergenic
GCGAGAGAGGAGAGGGAGTG+CGG 0.575299 7:-54241452 None:intergenic
TATCATTCTCATTCTTCTCG+GGG 0.576332 7:-54243836 None:intergenic
CTCAAGTATCTCGAGATCCG+AGG 0.584465 7:+54244138 MsG0780038861.01.T01:CDS
CAAAATGAGTGGTTTCATTG+TGG 0.586542 7:-54241534 None:intergenic
TTTGGATGAAAACCTGCAGA+AGG 0.596555 7:+54244110 MsG0780038861.01.T01:CDS
TCAAGTATCTCGAGATCCGA+GGG 0.601222 7:+54244139 MsG0780038861.01.T01:CDS
CTAAATGCTTACGTGAACTC+AGG 0.611960 7:-54244011 None:intergenic
TCTCCTCTCTCGCCGTGCAG+CGG 0.617355 7:+54241462 MsG0780038861.01.T01:CDS
ATCTCAAAAGGAAAATCACC+GGG 0.618030 7:-54243696 None:intergenic
TGGAGTGTGTATGGAGTTTG+GGG 0.622811 7:+54243902 MsG0780038861.01.T01:CDS
TGCGCTGTGGAAGGTTCTGG+AGG 0.628252 7:-54241491 None:intergenic
GAAGACCAGCTGGTATATGA+GGG 0.628639 7:+54243987 MsG0780038861.01.T01:CDS
GCTGCACGGCGAGAGAGGAG+AGG 0.631466 7:-54241460 None:intergenic
TCGAAGGAGAGTATCATCAG+CGG 0.634573 7:-54241599 None:intergenic
CGTGGCACCTCAACAGAGTG+AGG 0.643427 7:+54243803 MsG0780038861.01.T01:CDS
TATCAATCACAACTTCATCA+GGG 0.644367 7:-54243935 None:intergenic
CATGTTCAACAAAGACAACA+AGG 0.647062 7:+54244197 MsG0780038861.01.T01:CDS
TTTCACTGTCAAGGACTCGA+AGG 0.655034 7:-54241615 None:intergenic
ACAACAAGGAATACTTGGGG+TGG 0.664017 7:+54244211 MsG0780038861.01.T01:CDS
GGTTAACGGCGATTGCGCTG+TGG 0.671069 7:-54241504 None:intergenic
ACGAGAGGGTACAAAATGAG+TGG 0.672739 7:-54241545 None:intergenic
AAGGAGAGTATCATCAGCGG+GGG 0.679029 7:-54241596 None:intergenic
GCCGTAAGCGAAGCTACGAG+AGG 0.685303 7:-54241560 None:intergenic
TCAAGTTGACATCCCTAACG+TGG 0.692064 7:+54243785 MsG0780038861.01.T01:CDS
CCGTAAGCGAAGCTACGAGA+GGG 0.698699 7:-54241559 None:intergenic
GAAGGAGAGTATCATCAGCG+GGG 0.713416 7:-54241597 None:intergenic
AAGACAACAAGGAATACTTG+GGG 0.756411 7:+54244208 MsG0780038861.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAACTTAAAAACAA+AGG - Chr7:54241702-54241721 None:intergenic 10.0%
!! AAAAAAAACTTAAAAACAAA+GGG - Chr7:54241701-54241720 None:intergenic 10.0%
!!! TTCAATTTTTTTTTTTTGTT+GGG + Chr7:54241793-54241812 MsG0780038861.01.T01:intron 10.0%
!!! TTTCAATTTTTTTTTTTTGT+TGG + Chr7:54241792-54241811 MsG0780038861.01.T01:intron 10.0%
!! AAGATGTAAAAATTCTATAA+TGG + Chr7:54241984-54242003 MsG0780038861.01.T01:intron 15.0%
!!! AAAAAATTACGTTTGTTAAA+AGG - Chr7:54241672-54241691 None:intergenic 15.0%
!! AAAAACAAAACTTCACAAAA+GGG - Chr7:54241868-54241887 None:intergenic 20.0%
!! AAATAGGTCATTTAAACATA+CGG - Chr7:54242910-54242929 None:intergenic 20.0%
!! AAATATAAACAAAAATGGAG+TGG - Chr7:54242883-54242902 None:intergenic 20.0%
!! AATGAGCAATTTACATATAA+TGG - Chr7:54243070-54243089 None:intergenic 20.0%
!! ATGAGCAATTTACATATAAT+GGG - Chr7:54243069-54243088 None:intergenic 20.0%
!! GAGTGAAATATAAACAAAAA+TGG - Chr7:54242888-54242907 None:intergenic 20.0%
!! TAAAAACAAAACTTCACAAA+AGG - Chr7:54241869-54241888 None:intergenic 20.0%
!! TAAATCCAGACTTAAAAAAT+AGG - Chr7:54242926-54242945 None:intergenic 20.0%
!!! ATTTACATATAATGGGTAAA+AGG - Chr7:54243062-54243081 None:intergenic 20.0%
!!! TAGAATTTTTACATCTTACA+AGG - Chr7:54241981-54242000 None:intergenic 20.0%
!!! TGAAGTTTTGTTTTTATTAG+TGG + Chr7:54241873-54241892 MsG0780038861.01.T01:intron 20.0%
!!! TTTAGTTGATGTAAATAAAG+TGG + Chr7:54242590-54242609 MsG0780038861.01.T01:intron 20.0%
! AAAACAAAACTTCACAAAAG+GGG - Chr7:54241867-54241886 None:intergenic 25.0%
! AAAACATGCTATAACTTGAT+TGG + Chr7:54243027-54243046 MsG0780038861.01.T01:intron 25.0%
! AAGAGATATTCAAATCTACA+AGG - Chr7:54243303-54243322 None:intergenic 25.0%
! CAACTTTCAGACTAAAAAAA+AGG - Chr7:54243337-54243356 None:intergenic 25.0%
! GGATCTTAATAGAATGATAA+GGG + Chr7:54241894-54241913 MsG0780038861.01.T01:intron 25.0%
! TCAAATATTGTTTATTGCTC+TGG + Chr7:54243089-54243108 MsG0780038861.01.T01:intron 25.0%
! TGGATCTTAATAGAATGATA+AGG + Chr7:54241893-54241912 MsG0780038861.01.T01:intron 25.0%
! TTCCACTGAAAAAATGATAT+TGG + Chr7:54242528-54242547 MsG0780038861.01.T01:intron 25.0%
! TTGATGTAAATAAAGTGGAA+TGG + Chr7:54242595-54242614 MsG0780038861.01.T01:intron 25.0%
!! AATCAAGTTATAGCATGTTT+TGG - Chr7:54243028-54243047 None:intergenic 25.0%
!! AGAATGATAAGGGTTTTTAT+AGG + Chr7:54241904-54241923 MsG0780038861.01.T01:intron 25.0%
!! AGTGTCAATAATATTTCTAC+CGG + Chr7:54242764-54242783 MsG0780038861.01.T01:intron 25.0%
!! CATGTTCTGAACATTATTAT+TGG + Chr7:54242136-54242155 MsG0780038861.01.T01:intron 25.0%
!! GAATGATAAGGGTTTTTATA+GGG + Chr7:54241905-54241924 MsG0780038861.01.T01:intron 25.0%
!! TCATTTTCTCGAAATATTAC+TGG + Chr7:54242211-54242230 MsG0780038861.01.T01:intron 25.0%
!! TTAAGTTCTTGATCTTTTAG+TGG + Chr7:54242844-54242863 MsG0780038861.01.T01:intron 25.0%
!! TTTTACATCTTACAAGGAAA+TGG - Chr7:54241975-54241994 None:intergenic 25.0%
!!! AATGACCTATTTTTTAAGTC+TGG + Chr7:54242918-54242937 MsG0780038861.01.T01:intron 25.0%
!!! ATCCAATATCATTTTTTCAG+TGG - Chr7:54242533-54242552 None:intergenic 25.0%
!!! TTTTTCAATTATTTGCGTGT+AGG + Chr7:54242976-54242995 MsG0780038861.01.T01:intron 25.0%
AAAGACAACAAGGAATACTT+GGG + Chr7:54244207-54244226 MsG0780038861.01.T01:CDS 30.0%
CATGTATTCCTGTAAAAAGT+TGG - Chr7:54244181-54244200 None:intergenic 30.0%
TATCAATCACAACTTCATCA+GGG - Chr7:54243938-54243957 None:intergenic 30.0%
TTACAAGGAAATGGAACAAA+GGG - Chr7:54241966-54241985 None:intergenic 30.0%
TTGAAAACAGTCACAACTAA+AGG - Chr7:54243876-54243895 None:intergenic 30.0%
! AAGATTTGTCTTTTGTAGTC+TGG + Chr7:54242691-54242710 MsG0780038861.01.T01:intron 30.0%
! AGATTTGTCTTTTGTAGTCT+GGG + Chr7:54242692-54242711 MsG0780038861.01.T01:intron 30.0%
! TGTAAAAAGTTGGTTGTGTT+GGG - Chr7:54244171-54244190 None:intergenic 30.0%
! TGTAGCAGAATTTGAACTTT+TGG - Chr7:54242469-54242488 None:intergenic 30.0%
! TTAGTTGTGACTGTTTTCAA+AGG + Chr7:54243876-54243895 MsG0780038861.01.T01:CDS 30.0%
!! TGTTCATTTTTACAGCGATT+TGG + Chr7:54244092-54244111 MsG0780038861.01.T01:intron 30.0%
!!! GGATTTATTTTTGTCTGTCT+GGG + Chr7:54242939-54242958 MsG0780038861.01.T01:intron 30.0%
!!! TGGATTTATTTTTGTCTGTC+TGG + Chr7:54242938-54242957 MsG0780038861.01.T01:intron 30.0%
AAGACAACAAGGAATACTTG+GGG + Chr7:54244208-54244227 MsG0780038861.01.T01:CDS 35.0%
ACACTGTAGTGTTATATCAC+TGG + Chr7:54242402-54242421 MsG0780038861.01.T01:intron 35.0%
ACACTTCAGATCAAATCCTA+AGG + Chr7:54242042-54242061 MsG0780038861.01.T01:intron 35.0%
ACTTTGAGCTACATTCAAAG+TGG - Chr7:54242565-54242584 None:intergenic 35.0%
AGATTCATCCGGATTCTTTA+TGG - Chr7:54243967-54243986 None:intergenic 35.0%
AGTTGAATAGTTCTTTCTCC+AGG - Chr7:54243732-54243751 None:intergenic 35.0%
ATATACTGATGATTGGCACT+AGG + Chr7:54243478-54243497 MsG0780038861.01.T01:intron 35.0%
ATCTCAAAAGGAAAATCACC+GGG - Chr7:54243699-54243718 None:intergenic 35.0%
ATTGTTTATTGCTCTGGTTG+AGG + Chr7:54243095-54243114 MsG0780038861.01.T01:intron 35.0%
CAAAATGAGTGGTTTCATTG+TGG - Chr7:54241537-54241556 None:intergenic 35.0%
CAAAGACAACAAGGAATACT+TGG + Chr7:54244206-54244225 MsG0780038861.01.T01:CDS 35.0%
CAAGCAGTGTTGTCAAATAT+CGG - Chr7:54242500-54242519 None:intergenic 35.0%
CAATTGTGTTGTGTGTATGT+GGG + Chr7:54242628-54242647 MsG0780038861.01.T01:intron 35.0%
CATGTGCAAAACTATGATAC+CGG - Chr7:54242786-54242805 None:intergenic 35.0%
CATGTTCAACAAAGACAACA+AGG + Chr7:54244197-54244216 MsG0780038861.01.T01:CDS 35.0%
CATTATCTGCAGATTATCTG+TGG + Chr7:54243392-54243411 MsG0780038861.01.T01:intron 35.0%
CTATCAATCACAACTTCATC+AGG - Chr7:54243939-54243958 None:intergenic 35.0%
CTATCATTCTCATTCTTCTC+GGG - Chr7:54243840-54243859 None:intergenic 35.0%
CTTACAAGGAAATGGAACAA+AGG - Chr7:54241967-54241986 None:intergenic 35.0%
GAGTGTCATATACTGATGAT+TGG + Chr7:54243471-54243490 MsG0780038861.01.T01:intron 35.0%
GATCTCAAAAGGAAAATCAC+CGG - Chr7:54243700-54243719 None:intergenic 35.0%
GCAGCAAATCAAAGAATCTT+TGG - Chr7:54243265-54243284 None:intergenic 35.0%
GCTATCATTCTCATTCTTCT+CGG - Chr7:54243841-54243860 None:intergenic 35.0%
GCTCTTGAGGATAATAATGA+AGG + Chr7:54241646-54241665 MsG0780038861.01.T01:CDS 35.0%
GTTGAATAGTTCTTTCTCCA+GGG - Chr7:54243731-54243750 None:intergenic 35.0%
TAGTTTACTCGTGTTGTCAA+TGG + Chr7:54242261-54242280 MsG0780038861.01.T01:intron 35.0%
TATCATTCTCATTCTTCTCG+GGG - Chr7:54243839-54243858 None:intergenic 35.0%
TCAATGTGTCAGTACTTCAT+AGG + Chr7:54242161-54242180 MsG0780038861.01.T01:intron 35.0%
TCAATTGTGTTGTGTGTATG+TGG + Chr7:54242627-54242646 MsG0780038861.01.T01:intron 35.0%
TCTGTGGTATAAGAAAATGC+AGG + Chr7:54243408-54243427 MsG0780038861.01.T01:intron 35.0%
TTATAGGGTTGTTAGTGTTG+TGG + Chr7:54241920-54241939 MsG0780038861.01.T01:intron 35.0%
TTGTTTATTGCTCTGGTTGA+GGG + Chr7:54243096-54243115 MsG0780038861.01.T01:intron 35.0%
! AGCAGAATTTGAACTTTTGG+TGG - Chr7:54242466-54242485 None:intergenic 35.0%
! CAACACAACCAACTTTTTAC+AGG + Chr7:54244170-54244189 MsG0780038861.01.T01:CDS 35.0%
! CTGTAAAAAGTTGGTTGTGT+TGG - Chr7:54244172-54244191 None:intergenic 35.0%
! GTCTTCTTTTGCTACTCATT+TGG + Chr7:54242721-54242740 MsG0780038861.01.T01:intron 35.0%
! GTGACTGTTTTCAAAGGAAA+TGG + Chr7:54243882-54243901 MsG0780038861.01.T01:CDS 35.0%
! TAGCTTGTCCATAAAGAATC+CGG + Chr7:54243956-54243975 MsG0780038861.01.T01:CDS 35.0%
! TAGGATTTGATCTGAAGTGT+GGG - Chr7:54242042-54242061 None:intergenic 35.0%
! TTAGGATTTGATCTGAAGTG+TGG - Chr7:54242043-54242062 None:intergenic 35.0%
!! CAAAGAATCTTTGGAATCAC+TGG - Chr7:54243256-54243275 None:intergenic 35.0%
!! TACAGAGTGTTGTCAAATAG+TGG - Chr7:54242337-54242356 None:intergenic 35.0%
!! TGGTGTTATGTTTGGTATCA+TGG + Chr7:54243137-54243156 MsG0780038861.01.T01:intron 35.0%
!!! TGATTTTCCTTTTGAGATCG+AGG + Chr7:54243701-54243720 MsG0780038861.01.T01:CDS 35.0%
!!! TTATAGCATGTTTTGGCAAC+TGG - Chr7:54243021-54243040 None:intergenic 35.0%
AATGGAGTGTGTATGGAGTT+TGG + Chr7:54243900-54243919 MsG0780038861.01.T01:CDS 40.0%
AGTGTTGTGGTGAATGTTTG+TGG + Chr7:54241933-54241952 MsG0780038861.01.T01:intron 40.0%
ATCAAATCCTAAGGTGTGTC+CGG + Chr7:54242051-54242070 MsG0780038861.01.T01:intron 40.0%
ATGGAGTGTGTATGGAGTTT+GGG + Chr7:54243901-54243920 MsG0780038861.01.T01:CDS 40.0%
ATTGTGGGACAGATCTACAA+GGG - Chr7:54243546-54243565 None:intergenic 40.0%
CAAAGGAAATGGAGTGTGTA+TGG + Chr7:54243893-54243912 MsG0780038861.01.T01:CDS 40.0%
CAAGCAGTGTTGTCAAATGT+CGG - Chr7:54242433-54242452 None:intergenic 40.0%
CAATGACACATGTCAGATAC+CGG - Chr7:54242073-54242092 None:intergenic 40.0%
CTAAATGCTTACGTGAACTC+AGG - Chr7:54244014-54244033 None:intergenic 40.0%
GCAAAGGCTCAAAATCATAG+TGG - Chr7:54243199-54243218 None:intergenic 40.0%
TACTCGTGTTGTCAATGGAT+CGG + Chr7:54242266-54242285 MsG0780038861.01.T01:intron 40.0%
TATGTTTGGTATCATGGTGC+TGG + Chr7:54243143-54243162 MsG0780038861.01.T01:intron 40.0%
TGAAATCAACTGTGCTCTTG+AGG + Chr7:54241633-54241652 MsG0780038861.01.T01:CDS 40.0%
TTGTAGATCTGTCCCACAAT+CGG + Chr7:54243546-54243565 MsG0780038861.01.T01:intron 40.0%
TTTGGATGAAAACCTGCAGA+AGG + Chr7:54244110-54244129 MsG0780038861.01.T01:CDS 40.0%
! CACAGTTGATTTCACTGTCA+AGG - Chr7:54241627-54241646 None:intergenic 40.0%
! CTTTTCCAGCTTGAATTTCC+CGG + Chr7:54243678-54243697 MsG0780038861.01.T01:intron 40.0%
! GGTTTGTTTCAAGATCAGTC+TGG + Chr7:54243117-54243136 MsG0780038861.01.T01:intron 40.0%
!! GATCAGTCTGGTGTTATGTT+TGG + Chr7:54243129-54243148 MsG0780038861.01.T01:intron 40.0%
AATCACCGGGAAATTCAAGC+TGG - Chr7:54243686-54243705 None:intergenic 45.0%
ACAACAAGGAATACTTGGGG+TGG + Chr7:54244211-54244230 MsG0780038861.01.T01:CDS 45.0%
ACTCGTGTTGTCAATGGATC+GGG + Chr7:54242267-54242286 MsG0780038861.01.T01:intron 45.0%
ACTTGAGAAAAGCCTTCTGC+AGG - Chr7:54244125-54244144 None:intergenic 45.0%
ATTGTGGCGAAGGAGGTTAA+CGG - Chr7:54241521-54241540 None:intergenic 45.0%
CCCTGAAAAAGACCGATTGT+GGG - Chr7:54243561-54243580 None:intergenic 45.0%
GAACAACGACAGCAGTTATG+AGG - Chr7:54242814-54242833 None:intergenic 45.0%
GAAGACCAGCTGGTATATGA+GGG + Chr7:54243987-54244006 MsG0780038861.01.T01:CDS 45.0%
GATTGTGGGACAGATCTACA+AGG - Chr7:54243547-54243566 None:intergenic 45.0%
TAGTGCTACAGAGTCGGTAT+CGG - Chr7:54242016-54242035 None:intergenic 45.0%
TCAAAATCATAGTGGCTCCG+TGG - Chr7:54243191-54243210 None:intergenic 45.0%
TCAAGTATCTCGAGATCCGA+GGG + Chr7:54244139-54244158 MsG0780038861.01.T01:CDS 45.0%
TCTTGCGCTGTTACTAGTTC+AGG - Chr7:54242675-54242694 None:intergenic 45.0%
TGAAGACCAGCTGGTATATG+AGG + Chr7:54243986-54244005 MsG0780038861.01.T01:CDS 45.0%
TGGAGTGTGTATGGAGTTTG+GGG + Chr7:54243902-54243921 MsG0780038861.01.T01:CDS 45.0%
TTTCACTGTCAAGGACTCGA+AGG - Chr7:54241618-54241637 None:intergenic 45.0%
! ACGAGAGGGTACAAAATGAG+TGG - Chr7:54241548-54241567 None:intergenic 45.0%
! TCCCACAATCGGTCTTTTTC+AGG + Chr7:54243557-54243576 MsG0780038861.01.T01:intron 45.0%
! TTGTGTTGGGTGTTATCCCT+CGG - Chr7:54244158-54244177 None:intergenic 45.0%
!! AGCTGGTCTTCAGATTCATC+CGG - Chr7:54243978-54243997 None:intergenic 45.0%
!! CCCACAATCGGTCTTTTTCA+GGG + Chr7:54243558-54243577 MsG0780038861.01.T01:intron 45.0%
!! TCAAGTTGACATCCCTAACG+TGG + Chr7:54243785-54243804 MsG0780038861.01.T01:CDS 45.0%
!! TCGAAGGAGAGTATCATCAG+CGG - Chr7:54241602-54241621 None:intergenic 45.0%
!!! CCACAATCGGTCTTTTTCAG+GGG + Chr7:54243559-54243578 MsG0780038861.01.T01:intron 45.0%
ACCTCAACAGAGTGAGGATG+AGG + Chr7:54243809-54243828 MsG0780038861.01.T01:CDS 50.0%
CACATGTGTCAGTGTAGCGT+CGG - Chr7:54242099-54242118 None:intergenic 50.0%
CCCCTGAAAAAGACCGATTG+TGG - Chr7:54243562-54243581 None:intergenic 50.0%
CTCAAGTATCTCGAGATCCG+AGG + Chr7:54244138-54244157 MsG0780038861.01.T01:CDS 50.0%
CTCGTGTTGTCAATGGATCG+GGG + Chr7:54242268-54242287 MsG0780038861.01.T01:intron 50.0%
GAGCCACTGTGGTTTAGCAA+AGG - Chr7:54243215-54243234 None:intergenic 50.0%
GAGCCTTTGCTAAACCACAG+TGG + Chr7:54243209-54243228 MsG0780038861.01.T01:intron 50.0%
GAGTGGTTTCATTGTGGCGA+AGG - Chr7:54241531-54241550 None:intergenic 50.0%
GGATGAATCTGAAGACCAGC+TGG + Chr7:54243977-54243996 MsG0780038861.01.T01:CDS 50.0%
GGGTTGTCCTCGATCTCAAA+AGG - Chr7:54243711-54243730 None:intergenic 50.0%
TAAACCACAGTGGCTCACTG+CGG + Chr7:54243219-54243238 MsG0780038861.01.T01:intron 50.0%
TCAGATACCGGACACACCTT+AGG - Chr7:54242061-54242080 None:intergenic 50.0%
TCATAGTGGCTCCGTGGTTT+CGG - Chr7:54243185-54243204 None:intergenic 50.0%
TGGTTTCATTGTGGCGAAGG+AGG - Chr7:54241528-54241547 None:intergenic 50.0%
! GTTTGGTATCATGGTGCTGG+TGG + Chr7:54243146-54243165 MsG0780038861.01.T01:intron 50.0%
! TCTGTTGAGGTGCCACGTTA+GGG - Chr7:54243800-54243819 None:intergenic 50.0%
! TTTGAGATCGAGGACAACCC+TGG + Chr7:54243711-54243730 MsG0780038861.01.T01:CDS 50.0%
!! AAGGAGAGTATCATCAGCGG+GGG - Chr7:54241599-54241618 None:intergenic 50.0%
!! CGAAGGAGAGTATCATCAGC+GGG - Chr7:54241601-54241620 None:intergenic 50.0%
!! GAAGGAGAGTATCATCAGCG+GGG - Chr7:54241600-54241619 None:intergenic 50.0%
!!! ATCGGTCTTTTTCAGGGGTC+AGG + Chr7:54243564-54243583 MsG0780038861.01.T01:intron 50.0%
ACAGTGGCTCACTGCGGTTT+GGG + Chr7:54243225-54243244 MsG0780038861.01.T01:intron 55.0%
CAGGTCCCTCATATACCAGC+TGG - Chr7:54243995-54244014 None:intergenic 55.0%
CCCTCTCGTAGCTTCGCTTA+CGG + Chr7:54241559-54241578 MsG0780038861.01.T01:CDS 55.0%
CCGTAAGCGAAGCTACGAGA+GGG - Chr7:54241562-54241581 None:intergenic 55.0%
GATTGCGCTGTGGAAGGTTC+TGG - Chr7:54241497-54241516 None:intergenic 55.0%
GCCTCATCCTCACTCTGTTG+AGG - Chr7:54243813-54243832 None:intergenic 55.0%
GGGTGCTAGTGCTACAGAGT+CGG - Chr7:54242022-54242041 None:intergenic 55.0%
TAGTGGCGCTGTAGCACTGT+AGG - Chr7:54242320-54242339 None:intergenic 55.0%
TTGCGCCTTTGCCGAAACCA+CGG + Chr7:54243171-54243190 MsG0780038861.01.T01:intron 55.0%
! CTCTGTTGAGGTGCCACGTT+AGG - Chr7:54243801-54243820 None:intergenic 55.0%
AACGGCGATTGCGCTGTGGA+AGG - Chr7:54241503-54241522 None:intergenic 60.0%
CAAACCGCAGTGAGCCACTG+TGG - Chr7:54243226-54243245 None:intergenic 60.0%
CACAGTGGCTCACTGCGGTT+TGG + Chr7:54243224-54243243 MsG0780038861.01.T01:intron 60.0%
CGTGGCACCTCAACAGAGTG+AGG + Chr7:54243803-54243822 MsG0780038861.01.T01:CDS 60.0%
GCCGTAAGCGAAGCTACGAG+AGG - Chr7:54241563-54241582 None:intergenic 60.0%
GGTTAACGGCGATTGCGCTG+TGG - Chr7:54241507-54241526 None:intergenic 60.0%
TGGAGGAAGTTGCCGCTGCA+CGG - Chr7:54241477-54241496 None:intergenic 60.0%
TGGCTCCGTGGTTTCGGCAA+AGG - Chr7:54243179-54243198 None:intergenic 60.0%
! TGCGCTGTGGAAGGTTCTGG+AGG - Chr7:54241494-54241513 None:intergenic 60.0%
CTGCACGGCGAGAGAGGAGA+GGG - Chr7:54241462-54241481 None:intergenic 65.0%
GCGAGAGAGGAGAGGGAGTG+CGG - Chr7:54241455-54241474 None:intergenic 65.0%
TCTCCTCTCTCGCCGTGCAG+CGG + Chr7:54241462-54241481 MsG0780038861.01.T01:CDS 65.0%
! AGAGAGGAGAGGGAGTGCGG+CGG - Chr7:54241452-54241471 None:intergenic 65.0%
GCTGCACGGCGAGAGAGGAG+AGG - Chr7:54241463-54241482 None:intergenic 70.0%
TTGCCGCTGCACGGCGAGAG+AGG - Chr7:54241468-54241487 None:intergenic 70.0%
! GAGGAGAGGGAGTGCGGCGG+CGG - Chr7:54241449-54241468 None:intergenic 75.0%
! GGCGGTGGCGCGACGGAGAG+CGG - Chr7:54241431-54241450 None:intergenic 80.0%
!! GAGAGGGAGTGCGGCGGCGG+TGG - Chr7:54241446-54241465 None:intergenic 80.0%
! GTGCGGCGGCGGTGGCGCGA+CGG - Chr7:54241438-54241457 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr7 gene 54241415 54244266 54241415 ID=MsG0780038861.01;Name=MsG0780038861.01
Chr7 mRNA 54241415 54244266 54241415 ID=MsG0780038861.01.T01;Parent=MsG0780038861.01;Name=MsG0780038861.01.T01;_AED=0.38;_eAED=0.39;_QI=0|0|0|1|1|1|3|0|253
Chr7 exon 54241415 54241681 54241415 ID=MsG0780038861.01.T01:exon:15251;Parent=MsG0780038861.01.T01
Chr7 exon 54243687 54244021 54243687 ID=MsG0780038861.01.T01:exon:15252;Parent=MsG0780038861.01.T01
Chr7 exon 54244107 54244266 54244107 ID=MsG0780038861.01.T01:exon:15253;Parent=MsG0780038861.01.T01
Chr7 CDS 54241415 54241681 54241415 ID=MsG0780038861.01.T01:cds;Parent=MsG0780038861.01.T01
Chr7 CDS 54243687 54244021 54243687 ID=MsG0780038861.01.T01:cds;Parent=MsG0780038861.01.T01
Chr7 CDS 54244107 54244266 54244107 ID=MsG0780038861.01.T01:cds;Parent=MsG0780038861.01.T01
Gene Sequence

>MsG0780038861.01.T01

ATGTCTATGTACACCGCTCTCCGTCGCGCCACCGCCGCCGCACTCCCTCTCCTCTCTCGCCGTGCAGCGGCAACTTCCTCCAGAACCTTCCACAGCGCAATCGCCGTTAACCTCCTTCGCCACAATGAAACCACTCATTTTGTACCCTCTCGTAGCTTCGCTTACGGCGTTTTAGCGAAAACCCCCGCTGATGATACTCTCCTTCGAGTCCTTGACAGTGAAATCAACTGTGCTCTTGAGGATAATAATGAAGGCCTTTTAACAAACCTTGAATTTCCCGGTGATTTTCCTTTTGAGATCGAGGACAACCCTGGAGAAAGAACTATTCAACTGAAAAGACAATTTGAAGATGAAACTATCACAGTTCAAGTTGACATCCCTAACGTGGCACCTCAACAGAGTGAGGATGAGGCTGACCCCGAGAAGAATGAGAATGATAGCGAATCTAGTATTCCTTTAGTTGTGACTGTTTTCAAAGGAAATGGAGTGTGTATGGAGTTTGGGGTGACTGCTTTCCCTGATGAAGTTGTGATTGATAGCTTGTCCATAAAGAATCCGGATGAATCTGAAGACCAGCTGGTATATGAGGGACCTGAGTTCACCGATTTGGATGAAAACCTGCAGAAGGCTTTTCTCAAGTATCTCGAGATCCGAGGGATAACACCCAACACAACCAACTTTTTACAGGAATACATGTTCAACAAAGACAACAAGGAATACTTGGGGTGGCTGAAGAAACTGAAGAGCTTCATTGAGAAATGA
Protein sequence

>MsG0780038861.01.T01

MSMYTALRRATAAALPLLSRRAAATSSRTFHSAIAVNLLRHNETTHFVPSRSFAYGVLAKTPADDTLLRVLDSEINCALEDNNEGLLTNLEFPGDFPFEIEDNPGERTIQLKRQFEDETITVQVDIPNVAPQQSEDEADPEKNENDSESSIPLVVTVFKGNGVCMEFGVTAFPDEVVIDSLSIKNPDESEDQLVYEGPEFTDLDENLQKAFLKYLEIRGITPNTTNFLQEYMFNKDNKEYLGWLKKLKSFIEK*