AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005543.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005543.01.T01 MTR_1g102540 81.319 182 8 2 6 169 11 184 4.85e-90 261
MsG0180005543.01.T01 MTR_1g102540 81.215 181 8 2 7 169 71 243 1.06e-88 260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005543.01.T01 AT5G19910 62.791 172 28 4 7 160 18 171 2.01e-57 179
MsG0180005543.01.T01 AT5G19910 53.731 201 27 5 7 159 18 200 6.01e-51 163

Find 51 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATCACTATTTACCTTAT+TGG 0.153158 1:-93065646 None:intergenic
GTAGTGCATGCACAGGTGGC+TGG 0.230176 1:-93066190 None:intergenic
GGAGTTGAAGTAATAATAAT+AGG 0.256428 1:+93066399 MsG0180005543.01.T01:three_prime_UTR
GGATCCTTGTATATATTCTT+TGG 0.257913 1:-93064335 None:intergenic
GAACCTAGTGCTGCGCTTCC+TGG 0.281659 1:+93066149 MsG0180005543.01.T01:CDS
AGAAAGTGAGTGCTTTATAT+TGG 0.289671 1:+93066346 MsG0180005543.01.T01:three_prime_UTR
AACCGTTGTCGCCCATCATC+TGG 0.352900 1:-93064356 None:intergenic
CTAGGTTCTGGAAGTGGTCT+TGG 0.382881 1:-93066134 None:intergenic
TAGTGCATGCACAGGTGGCT+GGG 0.384524 1:-93066189 None:intergenic
AATGGCACATCCTACCAATA+AGG 0.389767 1:+93065632 MsG0180005543.01.T01:CDS
GGAAGCGCAGCACTAGGTTC+TGG 0.393441 1:-93066146 None:intergenic
TGTGCTACCCAACTTAAAAT+AGG 0.393795 1:-93066475 None:intergenic
TCTACTTCTGGAAGAACTAT+AGG 0.406034 1:+93066087 MsG0180005543.01.T01:CDS
GAAATACTTCAGCTGATAGA+AGG 0.409099 1:+93066320 MsG0180005543.01.T01:three_prime_UTR
TTTGAGGACGAAGCATTCAT+TGG 0.411048 1:+93065254 MsG0180005543.01.T01:CDS
ATGCTTCGTCCTCAAAATAT+CGG 0.443322 1:-93065247 None:intergenic
TAGTGCTGCGCTTCCTGGTC+CGG 0.453206 1:+93066154 MsG0180005543.01.T01:CDS
ATAGACAGCAGTTCTACTTC+TGG 0.455964 1:+93066075 MsG0180005543.01.T01:CDS
AGTGCATGCACAGGTGGCTG+GGG 0.456575 1:-93066188 None:intergenic
GGTTGAGTCGAGGCCGGACC+AGG 0.460454 1:-93066167 None:intergenic
GGCTGGGGTTGAGTCGAGGC+CGG 0.471060 1:-93066173 None:intergenic
TCACTATTTACCTTATTGGT+AGG 0.479734 1:-93065642 None:intergenic
GGAAGAACTATAGGAACAAT+CGG 0.500395 1:+93066096 MsG0180005543.01.T01:CDS
GAGTTGAAGTAATAATAATA+GGG 0.503829 1:+93066400 MsG0180005543.01.T01:three_prime_UTR
TTGAGGACGAAGCATTCATT+GGG 0.512149 1:+93065255 MsG0180005543.01.T01:CDS
TCTAACGAGTTTCAACCGTC+TGG 0.512281 1:-93066519 None:intergenic
ATACTTTCTTCCATCGCAGA+TGG 0.517896 1:+93066377 MsG0180005543.01.T01:three_prime_UTR
GCAACACTTGTAGCAGGTAC+TGG 0.519969 1:-93066215 None:intergenic
GAAGAAGCTCAAGAAAATAG+AGG 0.520705 1:-93065587 None:intergenic
TCCTACCTACATCCACTGTA+TGG 0.521585 1:+93064415 MsG0180005543.01.T01:CDS
ATATACAAGGATCCAGATGA+TGG 0.525951 1:+93064344 MsG0180005543.01.T01:CDS
GGACCAGGAAGCGCAGCACT+AGG 0.549090 1:-93066152 None:intergenic
GCAGCACTAGGTTCTGGAAG+TGG 0.553833 1:-93066140 None:intergenic
ATACCGTATGGCATTGGAGA+AGG 0.568373 1:-93066263 None:intergenic
TACTTTCTTCCATCGCAGAT+GGG 0.571187 1:+93066378 MsG0180005543.01.T01:three_prime_UTR
GAAAACCATACAGTGGATGT+AGG 0.571854 1:-93064420 None:intergenic
AGGTGGCTGGGGTTGAGTCG+AGG 0.574599 1:-93066177 None:intergenic
GTCACAGCAACACTTGTAGC+AGG 0.586975 1:-93066221 None:intergenic
ACCATACAGTGGATGTAGGT+AGG 0.594759 1:-93064416 None:intergenic
ATCCAGATGATGGGCGACAA+CGG 0.595215 1:+93064354 MsG0180005543.01.T01:CDS
GCTCCTTCTCCAATGCCATA+CGG 0.595355 1:+93066260 MsG0180005543.01.T01:CDS
ACTTCAACTCCCATCTGCGA+TGG 0.598476 1:-93066387 None:intergenic
GGTGGTAGTGCATGCACAGG+TGG 0.607758 1:-93066194 None:intergenic
TATACAAGGATCCAGATGAT+GGG 0.614053 1:+93064345 MsG0180005543.01.T01:CDS
TAGGCCAAAGAATATATACA+AGG 0.644719 1:+93064331 MsG0180005543.01.T01:CDS
TCAAGAAAATAGAGGCAGTG+AGG 0.646000 1:-93065579 None:intergenic
GGATGTAGGTAGGATTAGCA+AGG 0.649374 1:-93064406 None:intergenic
ATTGAAAATACATATCCAGA+CGG 0.649827 1:+93066504 MsG0180005543.01.T01:three_prime_UTR
ACTGGTGGTAGTGCATGCAC+AGG 0.653824 1:-93066197 None:intergenic
ATAAAATGAAAACCATACAG+TGG 0.657483 1:-93064427 None:intergenic
ACACTTGTAGCAGGTACTGG+TGG 0.693490 1:-93066212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAAATTTAATATATTC+AGG - Chr1:93065311-93065330 None:intergenic 10.0%
!! TGATAAATTTAATATATTCA+GGG - Chr1:93065310-93065329 None:intergenic 10.0%
!!! AATTTCAAATTATCTTTTAA+TGG + Chr1:93066574-93066593 MsG0180005543.01.T01:three_prime_UTR 10.0%
!!! GAAATAATTTTACAATTCAT+TGG - Chr1:93066551-93066570 None:intergenic 15.0%
!!! TTTATTTAATTTCAAGTACA+TGG + Chr1:93064642-93064661 MsG0180005543.01.T01:intron 15.0%
!! AATACTATAATATGAAATCC+AGG - Chr1:93065702-93065721 None:intergenic 20.0%
!! AATTGTTTAGTTAGAAAACA+AGG + Chr1:93064531-93064550 MsG0180005543.01.T01:intron 20.0%
!! ATGTTGGAATTATAAGTAAT+CGG + Chr1:93064572-93064591 MsG0180005543.01.T01:intron 20.0%
!! GAGTTGAAGTAATAATAATA+GGG + Chr1:93066400-93066419 MsG0180005543.01.T01:three_prime_UTR 20.0%
!!! ACTTAAAGTATCTTCAATAT+TGG + Chr1:93065279-93065298 MsG0180005543.01.T01:intron 20.0%
!!! AGAAATCATGATTTTGTAAA+AGG - Chr1:93065679-93065698 None:intergenic 20.0%
!!! ATGTTTTACAATTCTACTTA+AGG + Chr1:93065378-93065397 MsG0180005543.01.T01:intron 20.0%
!!! ATTACTTGCATTTTAAGTTT+AGG + Chr1:93065992-93066011 MsG0180005543.01.T01:intron 20.0%
!!! TGTTTTACAATTCTACTTAA+GGG + Chr1:93065379-93065398 MsG0180005543.01.T01:intron 20.0%
! AACTTAAAATGCAAGTAATC+TGG - Chr1:93065991-93066010 None:intergenic 25.0%
! ATAAAATGAAAACCATACAG+TGG - Chr1:93064430-93064449 None:intergenic 25.0%
! ATTGAAAATACATATCCAGA+CGG + Chr1:93066504-93066523 MsG0180005543.01.T01:three_prime_UTR 25.0%
! ATTGTAATTTCTCTTAATCC+AGG + Chr1:93065088-93065107 MsG0180005543.01.T01:intron 25.0%
! GATATCACTATTTACCTTAT+TGG - Chr1:93065649-93065668 None:intergenic 25.0%
! GCACTGAATTATGTTAAATA+GGG + Chr1:93065401-93065420 MsG0180005543.01.T01:intron 25.0%
! GGAGTTGAAGTAATAATAAT+AGG + Chr1:93066399-93066418 MsG0180005543.01.T01:three_prime_UTR 25.0%
! TAGAATAAAAATGACAGAAC+TGG - Chr1:93065494-93065513 None:intergenic 25.0%
!! ACTCTATAAGAATTTTACAC+AGG + Chr1:93065426-93065445 MsG0180005543.01.T01:intron 25.0%
!! CTCTATAAGAATTTTACACA+GGG + Chr1:93065427-93065446 MsG0180005543.01.T01:intron 25.0%
!! TCTGGATATGTATTTTCAAT+TGG - Chr1:93066504-93066523 None:intergenic 25.0%
!! TTCCTACAACAGATAAATTT+TGG - Chr1:93066048-93066067 None:intergenic 25.0%
!!! AGATTATTCTAGAGTAGATT+AGG + Chr1:93064610-93064629 MsG0180005543.01.T01:intron 25.0%
!!! CATGTTTTTTTGTCAAAAAG+GGG - Chr1:93065121-93065140 None:intergenic 25.0%
!!! CCATGTTTTTTTGTCAAAAA+GGG - Chr1:93065122-93065141 None:intergenic 25.0%
!!! TTACAATGTGTTTTCAAGAA+AGG - Chr1:93065075-93065094 None:intergenic 25.0%
AGAAAGTGAGTGCTTTATAT+TGG + Chr1:93066346-93066365 MsG0180005543.01.T01:three_prime_UTR 30.0%
CAAGGAATAAGTTGTGAAAA+AGG + Chr1:93064549-93064568 MsG0180005543.01.T01:intron 30.0%
GACCAAAATTTATCTGTTGT+AGG + Chr1:93066043-93066062 MsG0180005543.01.T01:intron 30.0%
GGATCCTTGTATATATTCTT+TGG - Chr1:93064338-93064357 None:intergenic 30.0%
TAAGTTGTGAAAAAGGATGT+TGG + Chr1:93064556-93064575 MsG0180005543.01.T01:intron 30.0%
TACAAAATCATGATTTCTCC+TGG + Chr1:93065681-93065700 MsG0180005543.01.T01:intron 30.0%
TAGGCCAAAGAATATATACA+AGG + Chr1:93064331-93064350 MsG0180005543.01.T01:CDS 30.0%
TCACTATTTACCTTATTGGT+AGG - Chr1:93065645-93065664 None:intergenic 30.0%
TCCTCAATACATCTATTAAG+CGG - Chr1:93065047-93065066 None:intergenic 30.0%
TGAGAGAATATGATAGATGA+AGG - Chr1:93064777-93064796 None:intergenic 30.0%
! CCCTTTTTGACAAAAAAACA+TGG + Chr1:93065119-93065138 MsG0180005543.01.T01:intron 30.0%
! CTAATAGTTCTTTGTTTGCT+TGG + Chr1:93065939-93065958 MsG0180005543.01.T01:intron 30.0%
! GGCACTGAATTATGTTAAAT+AGG + Chr1:93065400-93065419 MsG0180005543.01.T01:intron 30.0%
! GTTACTCTTTTGAACAGATT+TGG + Chr1:93065217-93065236 MsG0180005543.01.T01:intron 30.0%
! TACAACAAAATTTGTTCTCG+AGG - Chr1:93065764-93065783 None:intergenic 30.0%
!! TTCTGTGACCTATTTTAAGT+TGG + Chr1:93066467-93066486 MsG0180005543.01.T01:three_prime_UTR 30.0%
!!! GCCATGTTTTTTTGTCAAAA+AGG - Chr1:93065123-93065142 None:intergenic 30.0%
!!! GCTCATTTTTTATGAATGCT+AGG + Chr1:93065553-93065572 MsG0180005543.01.T01:intron 30.0%
!!! TCTGTGACCTATTTTAAGTT+GGG + Chr1:93066468-93066487 MsG0180005543.01.T01:three_prime_UTR 30.0%
AAGTACATGGTCTGTTTAAC+GGG + Chr1:93064655-93064674 MsG0180005543.01.T01:intron 35.0%
ATATACAAGGATCCAGATGA+TGG + Chr1:93064344-93064363 MsG0180005543.01.T01:CDS 35.0%
ATGCTTCGTCCTCAAAATAT+CGG - Chr1:93065250-93065269 None:intergenic 35.0%
CAAGTACATGGTCTGTTTAA+CGG + Chr1:93064654-93064673 MsG0180005543.01.T01:intron 35.0%
GAAATACTTCAGCTGATAGA+AGG + Chr1:93066320-93066339 MsG0180005543.01.T01:three_prime_UTR 35.0%
GAAGAAGCTCAAGAAAATAG+AGG - Chr1:93065590-93065609 None:intergenic 35.0%
GACATTGAGTTTAGTGACAT+TGG + Chr1:93064690-93064709 MsG0180005543.01.T01:intron 35.0%
GGAAGAACTATAGGAACAAT+CGG + Chr1:93066096-93066115 MsG0180005543.01.T01:CDS 35.0%
GTCGAGTCATGTATTTGAAA+GGG + Chr1:93064502-93064521 MsG0180005543.01.T01:intron 35.0%
TACCTACTGCATCACAATTT+CGG - Chr1:93065861-93065880 None:intergenic 35.0%
TATACAAGGATCCAGATGAT+GGG + Chr1:93064345-93064364 MsG0180005543.01.T01:CDS 35.0%
TCCGCTTAATAGATGTATTG+AGG + Chr1:93065043-93065062 MsG0180005543.01.T01:intron 35.0%
TGTCGAGTCATGTATTTGAA+AGG + Chr1:93064501-93064520 MsG0180005543.01.T01:intron 35.0%
TGTGCTACCCAACTTAAAAT+AGG - Chr1:93066478-93066497 None:intergenic 35.0%
TTAAGCAATAGCTGCATATG+TGG + Chr1:93064477-93064496 MsG0180005543.01.T01:intron 35.0%
TTGAGACAGATAGACAGATA+TGG + Chr1:93065795-93065814 MsG0180005543.01.T01:intron 35.0%
TTTCTTTGCCTTGTGTTTCT+TGG + Chr1:93066017-93066036 MsG0180005543.01.T01:intron 35.0%
! ACATTGAGCTTTTGTGACAT+CGG + Chr1:93064856-93064875 MsG0180005543.01.T01:intron 35.0%
! TAGCGATGCTTTGTTCAAAT+GGG + Chr1:93065512-93065531 MsG0180005543.01.T01:intron 35.0%
! TCTACTTCTGGAAGAACTAT+AGG + Chr1:93066087-93066106 MsG0180005543.01.T01:CDS 35.0%
! TCTAGAGTAGATTAGGTGTT+TGG + Chr1:93064617-93064636 MsG0180005543.01.T01:intron 35.0%
! TGCAAATTTTCGCAATGCAA+TGG + Chr1:93065614-93065633 MsG0180005543.01.T01:CDS 35.0%
AATGGCACATCCTACCAATA+AGG + Chr1:93065632-93065651 MsG0180005543.01.T01:CDS 40.0%
ACATTGAGCCTTTGTGACAT+CGG - Chr1:93064819-93064838 None:intergenic 40.0%
ATACTTTCTTCCATCGCAGA+TGG + Chr1:93066377-93066396 MsG0180005543.01.T01:three_prime_UTR 40.0%
ATAGACAGCAGTTCTACTTC+TGG + Chr1:93066075-93066094 MsG0180005543.01.T01:CDS 40.0%
CTCAATGTCACGGAAACTAT+GGG + Chr1:93064831-93064850 MsG0180005543.01.T01:intron 40.0%
CTTATTGTCCCACTAAAGTC+TGG + Chr1:93065345-93065364 MsG0180005543.01.T01:intron 40.0%
GAAAACCATACAGTGGATGT+AGG - Chr1:93064423-93064442 None:intergenic 40.0%
TAAAGTCACCGATGTCACAA+AGG + Chr1:93064808-93064827 MsG0180005543.01.T01:intron 40.0%
TACCGAAATTGTGATGCAGT+AGG + Chr1:93065856-93065875 MsG0180005543.01.T01:intron 40.0%
TACTTTCTTCCATCGCAGAT+GGG + Chr1:93066378-93066397 MsG0180005543.01.T01:three_prime_UTR 40.0%
TCAAAAAGGGGAATTTCACC+TGG - Chr1:93065109-93065128 None:intergenic 40.0%
TCAAGAAAATAGAGGCAGTG+AGG - Chr1:93065582-93065601 None:intergenic 40.0%
TGGTCAATCCAAGAAACACA+AGG - Chr1:93066028-93066047 None:intergenic 40.0%
! CTAGCGATGCTTTGTTCAAA+TGG + Chr1:93065511-93065530 MsG0180005543.01.T01:intron 40.0%
! TTGAGGACGAAGCATTCATT+GGG + Chr1:93065255-93065274 MsG0180005543.01.T01:CDS 40.0%
! TTTGAGGACGAAGCATTCAT+TGG + Chr1:93065254-93065273 MsG0180005543.01.T01:CDS 40.0%
AACCCAACAGGGAAACTACT+TGG - Chr1:93065010-93065029 None:intergenic 45.0%
ACCATACAGTGGATGTAGGT+AGG - Chr1:93064419-93064438 None:intergenic 45.0%
ACCCAACAGGGAAACTACTT+GGG - Chr1:93065009-93065028 None:intergenic 45.0%
ACCCAAGTAGTTTCCCTGTT+GGG + Chr1:93065005-93065024 MsG0180005543.01.T01:intron 45.0%
ATCCCTCTTCCAGACTTTAG+TGG - Chr1:93065357-93065376 None:intergenic 45.0%
CATGCTTTGTCATTGCTGCT+TGG + Chr1:93065142-93065161 MsG0180005543.01.T01:intron 45.0%
GAGTTGCACTTGGATGACAT+TGG - Chr1:93064978-93064997 None:intergenic 45.0%
GCTCAATGTCACGGAAACTA+TGG + Chr1:93064830-93064849 MsG0180005543.01.T01:intron 45.0%
GGAAACTACTTGGGTGACAT+TGG - Chr1:93065000-93065019 None:intergenic 45.0%
GTCACAAAGGCTCAATGTCA+CGG + Chr1:93064821-93064840 MsG0180005543.01.T01:intron 45.0%
TCCCACTAAAGTCTGGAAGA+GGG + Chr1:93065352-93065371 MsG0180005543.01.T01:intron 45.0%
TCCCTCTTCCAGACTTTAGT+GGG - Chr1:93065356-93065375 None:intergenic 45.0%
TCCTACCTACATCCACTGTA+TGG + Chr1:93064415-93064434 MsG0180005543.01.T01:CDS 45.0%
TCTAACGAGTTTCAACCGTC+TGG - Chr1:93066522-93066541 None:intergenic 45.0%
TGTGACATCGGTGACTTTAG+TGG + Chr1:93064868-93064887 MsG0180005543.01.T01:intron 45.0%
! ACAACGGTTTTTGCTGGAAC+TGG + Chr1:93064370-93064389 MsG0180005543.01.T01:CDS 45.0%
! ATACCGTATGGCATTGGAGA+AGG - Chr1:93066266-93066285 None:intergenic 45.0%
! CGATGCTTTGTTCAAATGGG+TGG + Chr1:93065515-93065534 MsG0180005543.01.T01:intron 45.0%
! GGATGTAGGTAGGATTAGCA+AGG - Chr1:93064409-93064428 None:intergenic 45.0%
! GGCTCAGAACCGATATTTTG+AGG + Chr1:93065238-93065257 MsG0180005543.01.T01:CDS 45.0%
!! TCTGTTTAACGGGTTGCACT+TGG + Chr1:93064665-93064684 MsG0180005543.01.T01:intron 45.0%
!!! ATTTTTTGCACTACCGAGCC+AGG - Chr1:93066293-93066312 None:intergenic 45.0%
!!! TTTTTTGCACTACCGAGCCA+GGG - Chr1:93066292-93066311 None:intergenic 45.0%
ACTTCAACTCCCATCTGCGA+TGG - Chr1:93066390-93066409 None:intergenic 50.0%
CACCCAAGTAGTTTCCCTGT+TGG + Chr1:93065004-93065023 MsG0180005543.01.T01:intron 50.0%
GCAACACTTGTAGCAGGTAC+TGG - Chr1:93066218-93066237 None:intergenic 50.0%
GCTCCTTCTCCAATGCCATA+CGG + Chr1:93066260-93066279 MsG0180005543.01.T01:CDS 50.0%
GGTGACATTGGAGTTGCACT+TGG - Chr1:93064988-93065007 None:intergenic 50.0%
GTCACAGCAACACTTGTAGC+AGG - Chr1:93066224-93066243 None:intergenic 50.0%
GTCCCACTAAAGTCTGGAAG+AGG + Chr1:93065351-93065370 MsG0180005543.01.T01:intron 50.0%
! TTTTTGCACTACCGAGCCAG+GGG - Chr1:93066291-93066310 None:intergenic 50.0%
!! ACACTTGTAGCAGGTACTGG+TGG - Chr1:93066215-93066234 None:intergenic 50.0%
!! ATCCAGATGATGGGCGACAA+CGG + Chr1:93064354-93064373 MsG0180005543.01.T01:CDS 50.0%
!! CTAGGTTCTGGAAGTGGTCT+TGG - Chr1:93066137-93066156 None:intergenic 50.0%
AACCGTTGTCGCCCATCATC+TGG - Chr1:93064359-93064378 None:intergenic 55.0%
ATACGGTATACCCCCTGGCT+CGG + Chr1:93066277-93066296 MsG0180005543.01.T01:CDS 55.0%
ATGCCATACGGTATACCCCC+TGG + Chr1:93066272-93066291 MsG0180005543.01.T01:CDS 55.0%
CCTCTCAGTGCAACCCAACA+GGG - Chr1:93065021-93065040 None:intergenic 55.0%
TAGTGCATGCACAGGTGGCT+GGG - Chr1:93066192-93066211 None:intergenic 55.0%
! ACTGGTGGTAGTGCATGCAC+AGG - Chr1:93066200-93066219 None:intergenic 55.0%
! CCTGTTGGGTTGCACTGAGA+GGG + Chr1:93065019-93065038 MsG0180005543.01.T01:intron 55.0%
! GGGGGTATACCGTATGGCAT+TGG - Chr1:93066272-93066291 None:intergenic 55.0%
! TGGGCGACAACGGTTTTTGC+TGG + Chr1:93064364-93064383 MsG0180005543.01.T01:CDS 55.0%
! TTTTGCACTACCGAGCCAGG+GGG - Chr1:93066290-93066309 None:intergenic 55.0%
!! GCAGCACTAGGTTCTGGAAG+TGG - Chr1:93066143-93066162 None:intergenic 55.0%
AGTGCATGCACAGGTGGCTG+GGG - Chr1:93066191-93066210 None:intergenic 60.0%
CCCTCTCAGTGCAACCCAAC+AGG - Chr1:93065022-93065041 None:intergenic 60.0%
GAACCTAGTGCTGCGCTTCC+TGG + Chr1:93066149-93066168 MsG0180005543.01.T01:CDS 60.0%
GTAGTGCATGCACAGGTGGC+TGG - Chr1:93066193-93066212 None:intergenic 60.0%
TAGTGCTGCGCTTCCTGGTC+CGG + Chr1:93066154-93066173 MsG0180005543.01.T01:CDS 60.0%
! CCCTGTTGGGTTGCACTGAG+AGG + Chr1:93065018-93065037 MsG0180005543.01.T01:intron 60.0%
! GAGCCAGGGGGTATACCGTA+TGG - Chr1:93066278-93066297 None:intergenic 60.0%
! GGAAGCGCAGCACTAGGTTC+TGG - Chr1:93066149-93066168 None:intergenic 60.0%
! GGTGGTAGTGCATGCACAGG+TGG - Chr1:93066197-93066216 None:intergenic 60.0%
AGGTGGCTGGGGTTGAGTCG+AGG - Chr1:93066180-93066199 None:intergenic 65.0%
! GGACCAGGAAGCGCAGCACT+AGG - Chr1:93066155-93066174 None:intergenic 65.0%
GGCTGGGGTTGAGTCGAGGC+CGG - Chr1:93066176-93066195 None:intergenic 70.0%
GGTTGAGTCGAGGCCGGACC+AGG - Chr1:93066170-93066189 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 93064317 93066609 93064317 ID=MsG0180005543.01;Name=MsG0180005543.01
Chr1 mRNA 93064317 93066609 93064317 ID=MsG0180005543.01.T01;Parent=MsG0180005543.01;Name=MsG0180005543.01.T01;_AED=0.44;_eAED=0.46;_QI=0|0.33|0.25|1|0|0|4|290|171
Chr1 exon 93064317 93064455 93064317 ID=MsG0180005543.01.T01:exon:9989;Parent=MsG0180005543.01.T01
Chr1 exon 93065234 93065276 93065234 ID=MsG0180005543.01.T01:exon:9990;Parent=MsG0180005543.01.T01
Chr1 exon 93065575 93065653 93065575 ID=MsG0180005543.01.T01:exon:9991;Parent=MsG0180005543.01.T01
Chr1 exon 93066065 93066609 93066065 ID=MsG0180005543.01.T01:exon:9992;Parent=MsG0180005543.01.T01
Chr1 CDS 93064317 93064455 93064317 ID=MsG0180005543.01.T01:cds;Parent=MsG0180005543.01.T01
Chr1 CDS 93065234 93065276 93065234 ID=MsG0180005543.01.T01:cds;Parent=MsG0180005543.01.T01
Chr1 CDS 93065575 93065653 93065575 ID=MsG0180005543.01.T01:cds;Parent=MsG0180005543.01.T01
Chr1 CDS 93066065 93066319 93066065 ID=MsG0180005543.01.T01:cds;Parent=MsG0180005543.01.T01
Chr1 three_prime_UTR 93066320 93066609 93066320 ID=MsG0180005543.01.T01:three_prime_utr;Parent=MsG0180005543.01.T01
Gene Sequence

>MsG0180005543.01.T01

ATGGTGTTTTTCTCTAGGCCAAAGAATATATACAAGGATCCAGATGATGGGCGACAACGGTTTTTGCTGGAACTGGAATTTGTTCAATGCCTTGCTAATCCTACCTACATCCACTGTATGGTTTTCATTTTATCAATATATTTGGCTCAGAACCGATATTTTGAGGACGAAGCATTCATTGGGTATCCTCACTGCCTCTATTTTCTTGAGCTTCTTCAGAATGCAAATTTTCGCAATGCAATGGCACATCCTACCAATAAGGAATTGACACATAGACAGCAGTTCTACTTCTGGAAGAACTATAGGAACAATCGGCTAAAACACATTTTACCAAGACCACTTCCAGAACCTAGTGCTGCGCTTCCTGGTCCGGCCTCGACTCAACCCCAGCCACCTGTGCATGCACTACCACCAGTACCTGCTACAAGTGTTGCTGTGACAACATCGTCTAGTCAAGCTCCTTCTCCAATGCCATACGGTATACCCCCTGGCTCGGTAGTGCAAAAAATGACATGA
Protein sequence

>MsG0180005543.01.T01

MVFFSRPKNIYKDPDDGRQRFLLELEFVQCLANPTYIHCMVFILSIYLAQNRYFEDEAFIGYPHCLYFLELLQNANFRNAMAHPTNKELTHRQQFYFWKNYRNNRLKHILPRPLPEPSAALPGPASTQPQPPVHALPPVPATSVAVTTSSSQAPSPMPYGIPPGSVVQKMT*