AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017266.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017266.01.T01 MTR_3g104310 98.718 78 1 0 7 84 133 210 3.49e-55 169
MsG0380017266.01.T01 MTR_7g021790 48.684 76 38 1 1 75 85 160 2.73e-23 89.0
MsG0380017266.01.T01 MTR_4g051225 48.684 76 38 1 1 75 85 160 3.14e-23 88.6
MsG0380017266.01.T01 MTR_0002s0630 47.297 74 38 1 1 73 85 158 3.27e-20 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017266.01.T01 AT5G11770 94.872 78 4 0 7 84 141 218 7.28e-54 166
MsG0380017266.01.T01 ATCG00430 48.684 76 38 1 1 75 85 160 4.17e-23 88.2

Find 36 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGTTGATATTTATGTTCC+CGG 0.283336 3:-94242365 MsG0380017266.01.T01:CDS
ACCAAATGCCCGAGCCTAGA+TGG 0.309620 3:-94242472 MsG0380017266.01.T01:CDS
CCCATCTAGGCTCGGGCATT+TGG 0.315314 3:+94242471 None:intergenic
CTCAGCAGTTGGAGGGCAGC+CGG 0.406470 3:+94242346 None:intergenic
TGATCTTCTTCTGCAGTTGG+AGG 0.439435 3:+94242306 None:intergenic
GAATGCTGACAAATCATTGC+AGG 0.445448 3:-94242502 MsG0380017266.01.T01:CDS
GCCTAGATGGGTTGTCTCCA+TGG 0.454702 3:-94242459 MsG0380017266.01.T01:CDS
ACTGCTGAGGCTTTACTGTA+TGG 0.466993 3:-94242332 MsG0380017266.01.T01:CDS
TATTGATCTTCTTCTGCAGT+TGG 0.469979 3:+94242303 None:intergenic
AACTGCAGAAGAAGATCAAT+AGG 0.480409 3:-94242301 MsG0380017266.01.T01:CDS
CCGGGAACATAAATATCAAC+AGG 0.485170 3:+94242365 None:intergenic
TCCATGGGAAGTTGTGCTAA+TGG 0.486720 3:-94242443 MsG0380017266.01.T01:CDS
CCCATGGAGACAACCCATCT+AGG 0.504105 3:+94242458 None:intergenic
TCAGCAGTTGGAGGGCAGCC+GGG 0.508884 3:+94242347 None:intergenic
TACAGTAAAGCCTCAGCAGT+TGG 0.522747 3:+94242335 None:intergenic
CCAAATGCCCGAGCCTAGAT+GGG 0.537493 3:-94242471 MsG0380017266.01.T01:CDS
GAGACAACCCATCTAGGCTC+GGG 0.544154 3:+94242464 None:intergenic
CCTAGATGGGTTGTCTCCAT+GGG 0.544839 3:-94242458 MsG0380017266.01.T01:CDS
CGGCTGCCCTCCAACTGCTG+AGG 0.547600 3:-94242345 MsG0380017266.01.T01:CDS
TCCATTAGCACAACTTCCCA+TGG 0.554029 3:+94242442 None:intergenic
GGAAGTTGTGCTAATGGAGG+AGG 0.559773 3:-94242437 MsG0380017266.01.T01:CDS
GGAGACAACCCATCTAGGCT+CGG 0.561380 3:+94242463 None:intergenic
CTGTTGTCCGAGGATGTGAC+AGG 0.567271 3:-94242394 MsG0380017266.01.T01:CDS
GGCGCAAGGACTTCCTCCAT+TGG 0.568783 3:-94242280 MsG0380017266.01.T01:CDS
GAACGATCCTGTCACATCCT+CGG 0.578017 3:+94242387 None:intergenic
GCAAGGACTTCCTCCATTGG+TGG 0.590384 3:-94242277 MsG0380017266.01.T01:CDS
CTCACTGAGTCCACCAATGG+AGG 0.591840 3:+94242267 None:intergenic
GTAAAGCCTCAGCAGTTGGA+GGG 0.606304 3:+94242339 None:intergenic
AGTAAAGCCTCAGCAGTTGG+AGG 0.610038 3:+94242338 None:intergenic
GACCTCACTGAGTCCACCAA+TGG 0.613212 3:+94242264 None:intergenic
TACTCTTACTCTGTTGTCCG+AGG 0.620931 3:-94242404 MsG0380017266.01.T01:CDS
GAAGAAGATCAATAGGCGCA+AGG 0.632047 3:-94242294 MsG0380017266.01.T01:CDS
CTCCATTGGTGGACTCAGTG+AGG 0.646971 3:-94242266 None:intergenic
ATGGGAAGTTGTGCTAATGG+AGG 0.660590 3:-94242440 MsG0380017266.01.T01:CDS
GGACAACAGAGTAAGAGTAG+TGG 0.675431 3:+94242408 None:intergenic
AATGCTGACAAATCATTGCA+GGG 0.685106 3:-94242501 MsG0380017266.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACTGCAGAAGAAGATCAAT+AGG - Chr3:94242467-94242486 MsG0380017266.01.T01:CDS 35.0%
CCTGTTGATATTTATGTTCC+CGG - Chr3:94242403-94242422 MsG0380017266.01.T01:CDS 35.0%
TATTGATCTTCTTCTGCAGT+TGG + Chr3:94242468-94242487 None:intergenic 35.0%
CCGGGAACATAAATATCAAC+AGG + Chr3:94242406-94242425 None:intergenic 40.0%
ACTGCTGAGGCTTTACTGTA+TGG - Chr3:94242436-94242455 MsG0380017266.01.T01:CDS 45.0%
ATGGGAAGTTGTGCTAATGG+AGG - Chr3:94242328-94242347 MsG0380017266.01.T01:CDS 45.0%
GAAGAAGATCAATAGGCGCA+AGG - Chr3:94242474-94242493 MsG0380017266.01.T01:CDS 45.0%
GGACAACAGAGTAAGAGTAG+TGG + Chr3:94242363-94242382 None:intergenic 45.0%
TACAGTAAAGCCTCAGCAGT+TGG + Chr3:94242436-94242455 None:intergenic 45.0%
TACTCTTACTCTGTTGTCCG+AGG - Chr3:94242364-94242383 MsG0380017266.01.T01:CDS 45.0%
TCCATGGGAAGTTGTGCTAA+TGG - Chr3:94242325-94242344 MsG0380017266.01.T01:CDS 45.0%
TCCATTAGCACAACTTCCCA+TGG + Chr3:94242329-94242348 None:intergenic 45.0%
TGATCTTCTTCTGCAGTTGG+AGG + Chr3:94242465-94242484 None:intergenic 45.0%
AGTAAAGCCTCAGCAGTTGG+AGG + Chr3:94242433-94242452 None:intergenic 50.0%
GAACGATCCTGTCACATCCT+CGG + Chr3:94242384-94242403 None:intergenic 50.0%
GGAAGTTGTGCTAATGGAGG+AGG - Chr3:94242331-94242350 MsG0380017266.01.T01:CDS 50.0%
GTAAAGCCTCAGCAGTTGGA+GGG + Chr3:94242432-94242451 None:intergenic 50.0%
!! CCTAGATGGGTTGTCTCCAT+GGG - Chr3:94242310-94242329 MsG0380017266.01.T01:CDS 50.0%
ACCAAATGCCCGAGCCTAGA+TGG - Chr3:94242296-94242315 MsG0380017266.01.T01:CDS 55.0%
CCAAATGCCCGAGCCTAGAT+GGG - Chr3:94242297-94242316 MsG0380017266.01.T01:CDS 55.0%
CCCATGGAGACAACCCATCT+AGG + Chr3:94242313-94242332 None:intergenic 55.0%
CTGTTGTCCGAGGATGTGAC+AGG - Chr3:94242374-94242393 MsG0380017266.01.T01:CDS 55.0%
GAGACAACCCATCTAGGCTC+GGG + Chr3:94242307-94242326 None:intergenic 55.0%
GCAAGGACTTCCTCCATTGG+TGG - Chr3:94242491-94242510 MsG0380017266.01.T01:CDS 55.0%
GGAGACAACCCATCTAGGCT+CGG + Chr3:94242308-94242327 None:intergenic 55.0%
!! GCCTAGATGGGTTGTCTCCA+TGG - Chr3:94242309-94242328 MsG0380017266.01.T01:CDS 55.0%
CCCATCTAGGCTCGGGCATT+TGG + Chr3:94242300-94242319 None:intergenic 60.0%
GGCGCAAGGACTTCCTCCAT+TGG - Chr3:94242488-94242507 MsG0380017266.01.T01:CDS 60.0%
CTCAGCAGTTGGAGGGCAGC+CGG + Chr3:94242425-94242444 None:intergenic 65.0%
TCAGCAGTTGGAGGGCAGCC+GGG + Chr3:94242424-94242443 None:intergenic 65.0%
CGGCTGCCCTCCAACTGCTG+AGG - Chr3:94242423-94242442 MsG0380017266.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 94242268 94242522 94242268 ID=MsG0380017266.01;Name=MsG0380017266.01
Chr3 mRNA 94242268 94242522 94242268 ID=MsG0380017266.01.T01;Parent=MsG0380017266.01;Name=MsG0380017266.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|84
Chr3 exon 94242268 94242522 94242268 ID=MsG0380017266.01.T01:exon:4269;Parent=MsG0380017266.01.T01
Chr3 CDS 94242268 94242522 94242268 ID=MsG0380017266.01.T01:cds;Parent=MsG0380017266.01.T01
Gene Sequence

>MsG0380017266.01.T01

ATGCTGACAAATCATTGCAGGGTGTATGACCAAATGCCCGAGCCTAGATGGGTTGTCTCCATGGGAAGTTGTGCTAATGGAGGAGGATACTACCACTACTCTTACTCTGTTGTCCGAGGATGTGACAGGATCGTTCCTGTTGATATTTATGTTCCCGGCTGCCCTCCAACTGCTGAGGCTTTACTGTATGGACTCCTCCAACTGCAGAAGAAGATCAATAGGCGCAAGGACTTCCTCCATTGGTGGACTCAGTGA

Protein sequence

>MsG0380017266.01.T01

MLTNHCRVYDQMPEPRWVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLLQLQKKINRRKDFLHWWTQ*