Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006041.01.T01 | XP_013470311.1 | 96.19 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 6.96E-67 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006041.01.T01 | Q9SKC9 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.54E-49 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006041.01.T01 | A0A072VQV0 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 3.32e-67 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048687.01 | MsG0180006041.01 | 0.812129 | 4.951037e-51 | 3.121856e-48 |
| MsG0180006041.01 | MsG0280007440.01 | 0.817593 | 3.043019e-52 | 2.225461e-49 |
| MsG0180006041.01 | MsG0280011234.01 | 0.813706 | 2.234470e-51 | 1.469888e-48 |
| MsG0180006041.01 | MsG0380012073.01 | 0.800918 | 1.150494e-48 | 5.422832e-46 |
| MsG0180006041.01 | MsG0380016389.01 | 0.801740 | 7.807168e-49 | 3.756813e-46 |
| MsG0180006041.01 | MsG0480022960.01 | 0.812097 | 5.033116e-51 | 3.170761e-48 |
| MsG0180006041.01 | MsG0480023559.01 | 0.826228 | 3.044349e-54 | 2.837997e-51 |
| MsG0180006041.01 | MsG0580029132.01 | 0.807914 | 4.004947e-50 | 2.258389e-47 |
| MsG0180006041.01 | MsG0680032809.01 | 0.806370 | 8.502613e-50 | 4.606900e-47 |
| MsG0180006041.01 | MsG0780038587.01 | 0.841723 | 3.997464e-58 | 5.925521e-55 |
| MsG0180006041.01 | MsG0780040511.01 | 0.834815 | 2.410042e-56 | 2.892320e-53 |
| MsG0180006041.01 | MsG0880042349.01 | 0.802942 | 4.414404e-49 | 2.190283e-46 |
| MsG0180006041.01 | MsG0880043770.01 | 0.825444 | 4.671397e-54 | 4.258360e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006041.01.T01 | MTR_1g112480 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 8.43e-71 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006041.01.T01 | AT2G02050 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.59e-50 | 154 |
Find 25 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAAGCATTGGCGGGTT+TGG | 0.229519 | 1:+99249075 | None:intergenic |
| GATCAAGCATTGGCGGGTTT+GGG | 0.236660 | 1:+99249076 | None:intergenic |
| ACCCTCTGAAACGGGTTGTT+TGG | 0.368427 | 1:+99249117 | None:intergenic |
| CGTTCGTTCTCGCACTTCCA+TGG | 0.425940 | 1:+99249226 | None:intergenic |
| AAGAGGAGATGGTGGAAGCT+AGG | 0.454716 | 1:-99249327 | MsG0180006041.01.T01:CDS |
| ATAGCAGCACCCTCTGAAAC+GGG | 0.480710 | 1:+99249109 | None:intergenic |
| AATAGCAGCACCCTCTGAAA+CGG | 0.485923 | 1:+99249108 | None:intergenic |
| CTTGTCTGCATTTGTTGAGA+GGG | 0.492622 | 1:+99249263 | None:intergenic |
| GGTGGAAGCTAGGGTTCCAT+TGG | 0.505790 | 1:-99249317 | MsG0180006041.01.T01:CDS |
| GCTTGTCTGCATTTGTTGAG+AGG | 0.517488 | 1:+99249262 | None:intergenic |
| AAGCTGAATTCTATCTTCCA+TGG | 0.530156 | 1:-99249243 | MsG0180006041.01.T01:CDS |
| AGAGGAGATGGTGGAAGCTA+GGG | 0.534962 | 1:-99249326 | MsG0180006041.01.T01:CDS |
| ATCAAGCATTGGCGGGTTTG+GGG | 0.544039 | 1:+99249077 | None:intergenic |
| GAGAGGGATCAAGCATTGGC+GGG | 0.545254 | 1:+99249070 | None:intergenic |
| GTGCGAATACGAACTCGTCA+TGG | 0.547428 | 1:-99249188 | MsG0180006041.01.T01:CDS |
| AGAGAGGGATCAAGCATTGG+CGG | 0.549265 | 1:+99249069 | None:intergenic |
| TCCAAACAACCCGTTTCAGA+GGG | 0.555884 | 1:-99249118 | MsG0180006041.01.T01:CDS |
| GAATCGAAGATGGAATTTGA+AGG | 0.557443 | 1:-99249376 | None:intergenic |
| GAGAGAGAGGGATCAAGCAT+TGG | 0.561121 | 1:+99249066 | None:intergenic |
| TTCCAAACAACCCGTTTCAG+AGG | 0.569899 | 1:-99249119 | MsG0180006041.01.T01:CDS |
| TTGGCGGGTTTGGGGATGAG+AGG | 0.579099 | 1:+99249085 | None:intergenic |
| GATTGCGACACAAGAGGAGA+TGG | 0.580463 | 1:-99249338 | MsG0180006041.01.T01:CDS |
| CATTGATCTCTGTAAGCCAA+TGG | 0.586159 | 1:+99249301 | None:intergenic |
| TGCGACACAAGAGGAGATGG+TGG | 0.590865 | 1:-99249335 | MsG0180006041.01.T01:CDS |
| GAAGATGATTGCGACACAAG+AGG | 0.593830 | 1:-99249344 | MsG0180006041.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGCATTTTGATTTTCG+CGG | + | Chr1:99249308-99249327 | None:intergenic | 25.0% |
| AAGCTGAATTCTATCTTCCA+TGG | - | Chr1:99249202-99249221 | MsG0180006041.01.T01:CDS | 35.0% | |
| CATTGATCTCTGTAAGCCAA+TGG | + | Chr1:99249147-99249166 | None:intergenic | 40.0% | |
| ! | CTTGTCTGCATTTGTTGAGA+GGG | + | Chr1:99249185-99249204 | None:intergenic | 40.0% |
| GAAGATGATTGCGACACAAG+AGG | - | Chr1:99249101-99249120 | MsG0180006041.01.T01:CDS | 45.0% | |
| TCCAAACAACCCGTTTCAGA+GGG | - | Chr1:99249327-99249346 | MsG0180006041.01.T01:CDS | 45.0% | |
| TTCCAAACAACCCGTTTCAG+AGG | - | Chr1:99249326-99249345 | MsG0180006041.01.T01:CDS | 45.0% | |
| ! | AATAGCAGCACCCTCTGAAA+CGG | + | Chr1:99249340-99249359 | None:intergenic | 45.0% |
| ! | GCTTGTCTGCATTTGTTGAG+AGG | + | Chr1:99249186-99249205 | None:intergenic | 45.0% |
| GATTGCGACACAAGAGGAGA+TGG | - | Chr1:99249107-99249126 | MsG0180006041.01.T01:CDS | 50.0% | |
| GTGCGAATACGAACTCGTCA+TGG | - | Chr1:99249257-99249276 | MsG0180006041.01.T01:CDS | 50.0% | |
| !! | AAGAGGAGATGGTGGAAGCT+AGG | - | Chr1:99249118-99249137 | MsG0180006041.01.T01:CDS | 50.0% |
| !! | ACCCTCTGAAACGGGTTGTT+TGG | + | Chr1:99249331-99249350 | None:intergenic | 50.0% |
| !! | AGAGGAGATGGTGGAAGCTA+GGG | - | Chr1:99249119-99249138 | MsG0180006041.01.T01:CDS | 50.0% |
| !! | ATAGCAGCACCCTCTGAAAC+GGG | + | Chr1:99249339-99249358 | None:intergenic | 50.0% |
| CGTTCGTTCTCGCACTTCCA+TGG | + | Chr1:99249222-99249241 | None:intergenic | 55.0% | |
| GGTGGAAGCTAGGGTTCCAT+TGG | - | Chr1:99249128-99249147 | MsG0180006041.01.T01:CDS | 55.0% | |
| TGCGACACAAGAGGAGATGG+TGG | - | Chr1:99249110-99249129 | MsG0180006041.01.T01:CDS | 55.0% | |
| !! | TTGGCGGGTTTGGGGATGAG+AGG | + | Chr1:99249363-99249382 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 99249078 | 99249389 | 99249078 | ID=MsG0180006041.01;Name=MsG0180006041.01 |
| Chr1 | mRNA | 99249078 | 99249389 | 99249078 | ID=MsG0180006041.01.T01;Parent=MsG0180006041.01;Name=MsG0180006041.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr1 | exon | 99249078 | 99249389 | 99249078 | ID=MsG0180006041.01.T01:exon:14840;Parent=MsG0180006041.01.T01 |
| Chr1 | CDS | 99249078 | 99249389 | 99249078 | ID=MsG0180006041.01.T01:cds;Parent=MsG0180006041.01.T01 |
| Gene Sequence |
| Protein sequence |
