Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006606.01.T01 | AFK42939.1 | 95.652 | 69 | 3 | 0 | 1 | 69 | 21 | 89 | 9.44E-40 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006606.01.T01 | I3SRP7 | 95.652 | 69 | 3 | 0 | 1 | 69 | 21 | 89 | 4.51e-40 | 135 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048595.01 | MsG0280006606.01 | 0.800951 | 1.132450e-48 | 5.342287e-46 |
| MsG0180000337.01 | MsG0280006606.01 | 0.802144 | 6.450280e-49 | 3.136022e-46 |
| MsG0180004058.01 | MsG0280006606.01 | 0.800476 | 1.415434e-48 | 6.596918e-46 |
| MsG0280006606.01 | MsG0280007227.01 | 0.809895 | 1.509029e-50 | 8.967619e-48 |
| MsG0280006606.01 | MsG0280007912.01 | 0.833401 | 5.447060e-56 | 6.266094e-53 |
| MsG0280006606.01 | MsG0280008263.01 | 0.808762 | 2.641364e-50 | 1.523059e-47 |
| MsG0280006606.01 | MsG0280010047.01 | 0.807587 | 4.699278e-50 | 2.627422e-47 |
| MsG0280006606.01 | MsG0380017076.01 | 0.807089 | 5.993841e-50 | 3.308183e-47 |
| MsG0280006606.01 | MsG0480023360.01 | 0.812390 | 4.342837e-51 | 2.757827e-48 |
| MsG0280006606.01 | MsG0580025510.01 | 0.839924 | 1.184004e-57 | 1.659906e-54 |
| MsG0280006606.01 | MsG0880046170.01 | 0.800703 | 1.272783e-48 | 5.966099e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006606.01.T01 | MTR_2g011690 | 95.652 | 69 | 3 | 0 | 1 | 69 | 21 | 89 | 1.14e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGATTTCTTAGATCAATT+TGG | 0.263324 | 2:-3993889 | None:intergenic |
| CTGTCATGTATTTGAGCTGC+TGG | 0.374821 | 2:+3993863 | MsG0280006606.01.T01:CDS |
| AACTTCTTCAAAGCAAGTTA+AGG | 0.411883 | 2:+3993741 | MsG0280006606.01.T01:CDS |
| CTTCAAAGCAAGTTAAGGCT+AGG | 0.439718 | 2:+3993746 | MsG0280006606.01.T01:CDS |
| TGTCATGTATTTGAGCTGCT+GGG | 0.468830 | 2:+3993864 | MsG0280006606.01.T01:CDS |
| AATTCATCTTCTACTTTCAA+AGG | 0.482157 | 2:+3993796 | MsG0280006606.01.T01:CDS |
| TTCAAAGCAAGTTAAGGCTA+GGG | 0.484226 | 2:+3993747 | MsG0280006606.01.T01:CDS |
| AACGTGCTGAAGAGTCTCTT+AGG | 0.527974 | 2:+3993839 | MsG0280006606.01.T01:CDS |
| GTCATGTATTTGAGCTGCTG+GGG | 0.585519 | 2:+3993865 | MsG0280006606.01.T01:CDS |
| CAAAGGTAGCAACAGTGACA+AGG | 0.606257 | 2:+3993813 | MsG0280006606.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTCATCTTCTACTTTCAA+AGG | + | Chr2:3993796-3993815 | MsG0280006606.01.T01:CDS | 25.0% |
| AACTTCTTCAAAGCAAGTTA+AGG | + | Chr2:3993741-3993760 | MsG0280006606.01.T01:CDS | 30.0% | |
| TTCAAAGCAAGTTAAGGCTA+GGG | + | Chr2:3993747-3993766 | MsG0280006606.01.T01:CDS | 35.0% | |
| CTTCAAAGCAAGTTAAGGCT+AGG | + | Chr2:3993746-3993765 | MsG0280006606.01.T01:CDS | 40.0% | |
| TGTCATGTATTTGAGCTGCT+GGG | + | Chr2:3993864-3993883 | MsG0280006606.01.T01:CDS | 40.0% | |
| AACGTGCTGAAGAGTCTCTT+AGG | + | Chr2:3993839-3993858 | MsG0280006606.01.T01:CDS | 45.0% | |
| CAAAGGTAGCAACAGTGACA+AGG | + | Chr2:3993813-3993832 | MsG0280006606.01.T01:CDS | 45.0% | |
| CTGTCATGTATTTGAGCTGC+TGG | + | Chr2:3993863-3993882 | MsG0280006606.01.T01:CDS | 45.0% | |
| GTCATGTATTTGAGCTGCTG+GGG | + | Chr2:3993865-3993884 | MsG0280006606.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 3993688 | 3993897 | 3993688 | ID=MsG0280006606.01;Name=MsG0280006606.01 |
| Chr2 | mRNA | 3993688 | 3993897 | 3993688 | ID=MsG0280006606.01.T01;Parent=MsG0280006606.01;Name=MsG0280006606.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|69 |
| Chr2 | exon | 3993688 | 3993897 | 3993688 | ID=MsG0280006606.01.T01:exon:899;Parent=MsG0280006606.01.T01 |
| Chr2 | CDS | 3993688 | 3993897 | 3993688 | ID=MsG0280006606.01.T01:cds;Parent=MsG0280006606.01.T01 |
| Gene Sequence |
| Protein sequence |
