AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025510.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025510.01.T01 MTR_5g029910 90.119 253 22 1 1 250 1 253 1.92e-146 409
MsG0580025510.01.T01 MTR_7g098450 50.000 118 58 1 109 225 212 329 4.41e-38 136
MsG0580025510.01.T01 MTR_7g098450 50.000 118 58 1 109 225 235 352 4.68e-38 137
MsG0580025510.01.T01 MTR_4g114690 61.702 94 36 0 125 218 165 258 5.17e-38 134
MsG0580025510.01.T01 MTR_4g010250 49.180 122 62 0 119 240 121 242 3.17e-37 131
MsG0580025510.01.T01 MTR_7g099260 50.962 104 51 0 122 225 237 340 3.94e-35 129
MsG0580025510.01.T01 MTR_2g103795 49.091 110 56 0 119 228 136 245 9.55e-34 123
MsG0580025510.01.T01 MTR_1g073230 39.683 126 65 2 110 224 250 375 8.63e-27 107
MsG0580025510.01.T01 MTR_1g073230 37.956 137 75 2 97 224 95 230 1.78e-23 97.8
MsG0580025510.01.T01 MTR_2g103795 48.864 88 45 0 119 206 136 223 9.07e-24 95.9
MsG0580025510.01.T01 MTR_3g463450 34.314 102 67 0 117 218 394 495 8.57e-16 76.6
MsG0580025510.01.T01 MTR_5g082130 31.250 112 76 1 108 218 283 394 2.50e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025510.01.T01 AT4G17940 43.629 259 96 8 1 220 1 248 1.04e-52 172
MsG0580025510.01.T01 AT5G20190 47.423 97 51 0 125 221 159 255 1.47e-30 115
MsG0580025510.01.T01 AT4G32340 53.000 100 46 1 122 220 110 209 5.15e-30 112
MsG0580025510.01.T01 AT1G80130 48.980 98 50 0 124 221 167 264 2.30e-26 104
MsG0580025510.01.T01 AT1G04530 44.898 98 53 1 122 219 121 217 3.61e-23 96.3
MsG0580025510.01.T01 AT1G04530 30.400 125 67 2 118 222 186 310 6.14e-15 73.2
MsG0580025510.01.T01 AT1G07280 35.354 99 64 0 120 218 433 531 4.79e-15 74.7
MsG0580025510.01.T01 AT1G07280 35.354 99 64 0 120 218 433 531 4.79e-15 74.7
MsG0580025510.01.T01 AT1G07280 35.354 99 64 0 120 218 433 531 4.79e-15 74.7
MsG0580025510.01.T01 AT2G29670 35.354 99 64 0 120 218 418 516 1.62e-14 73.2
MsG0580025510.01.T01 AT2G29670 35.354 99 64 0 120 218 418 516 1.62e-14 73.2

Find 92 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATCAATTCACTGTATTT+TGG 0.161174 5:-19798573 MsG0580025510.01.T01:three_prime_UTR
CGCTTTACGGAAAGTTGATA+TGG 0.203106 5:-19799526 MsG0580025510.01.T01:CDS
ATTATAGGGTAGGGCGTTTC+TGG 0.207251 5:+19798246 None:intergenic
CAGTCTCTCTCTTCAATTAT+AGG 0.214326 5:+19798231 None:intergenic
AATACATATGAGTATCTAAA+AGG 0.241907 5:-19798079 MsG0580025510.01.T01:three_prime_UTR
GGTGAATTGCTTTCGCTTTA+CGG 0.272624 5:-19799539 MsG0580025510.01.T01:CDS
GTGAGAGCAGAGGAATATTA+TGG 0.317988 5:-19799590 MsG0580025510.01.T01:CDS
AGTCTCTCTCTTCAATTATA+GGG 0.324428 5:+19798232 None:intergenic
GGTTATGTGACAACAATAAA+TGG 0.341346 5:-19798307 MsG0580025510.01.T01:three_prime_UTR
AGTCCAAATGTTTACTTAAC+CGG 0.353627 5:-19798434 MsG0580025510.01.T01:three_prime_UTR
GAAAGCAATTCACCATCTTC+AGG 0.375992 5:+19799548 None:intergenic
CCGGTTGAGGATACCGGATT+CGG 0.388537 5:-19799869 MsG0580025510.01.T01:CDS
TGGAAATGCAGGGAAACTTT+TGG 0.390187 5:+19800013 None:intergenic
GGAAGCGGAGGAAGAGGAGG+AGG 0.390683 5:-19798671 MsG0580025510.01.T01:CDS
TCTCCGGTTAAGTAAACATT+TGG 0.391751 5:+19798431 None:intergenic
ACCGGATTCGGCGGAGGAGG+CGG 0.393617 5:-19799857 MsG0580025510.01.T01:CDS
TCCGCCTCCTCCGCCGAATC+CGG 0.405182 5:+19799856 None:intergenic
CTAATATGCAGCACGGTGTT+GGG 0.405986 5:-19798715 MsG0580025510.01.T01:intron
TCTAATATGCAGCACGGTGT+TGG 0.410479 5:-19798716 MsG0580025510.01.T01:intron
GAGTGGACCGGGTTTAGAAC+CGG 0.424474 5:+19800106 None:intergenic
GAGGATGAAGTGGTGGTTAA+CGG 0.444648 5:-19798652 MsG0580025510.01.T01:CDS
CGAGTTGAACCGGAGTGGAC+CGG 0.448414 5:+19800094 None:intergenic
CACATTTGACATTCTGAACT+CGG 0.450580 5:-19798367 MsG0580025510.01.T01:three_prime_UTR
GATACCGGATTCGGCGGAGG+AGG 0.455567 5:-19799860 MsG0580025510.01.T01:CDS
GATTCCGTCGGAATGTTTGT+CGG 0.460131 5:+19799985 None:intergenic
GGAAGAGGAGGAGGATGAAG+TGG 0.463731 5:-19798662 MsG0580025510.01.T01:CDS
TGAATGAAGCTTGGCGGGAG+AGG 0.465208 5:+19800065 None:intergenic
GTTTAGAACCGGGACGGAAC+CGG 0.466497 5:+19800117 None:intergenic
TTCATGTTGCTCCTGATGAT+AGG 0.466904 5:-19799454 MsG0580025510.01.T01:intron
GAGGGAGGTTCGAGTTGAAC+CGG 0.467848 5:+19800084 None:intergenic
TCCCGCCAAGCTTCATTCAC+TGG 0.472339 5:-19800061 MsG0580025510.01.T01:CDS
TTCCGTCCCGGTTCTAAACC+CGG 0.476767 5:-19800113 MsG0580025510.01.T01:CDS
GCTTTACGGAAAGTTGATAT+GGG 0.476855 5:-19799525 MsG0580025510.01.T01:CDS
AATGTATAATACCTATCATC+AGG 0.482652 5:+19799443 None:intergenic
CCGAATCCGGTATCCTCAAC+CGG 0.486419 5:+19799869 None:intergenic
CTATTCATCCCTAAAATCGT+TGG 0.486496 5:+19798030 None:intergenic
TCTCAGAATCTAAATCGAAT+CGG 0.489329 5:-19799905 MsG0580025510.01.T01:CDS
CTCTCTTCAATTATAGGGTA+GGG 0.491721 5:+19798237 None:intergenic
ACAGTCAACACCGGTGGAAA+CGG 0.493901 5:-19799815 MsG0580025510.01.T01:CDS
CCTTTCACCACCGTTTCCAC+CGG 0.494768 5:+19799805 None:intergenic
CCTCCGCTTCCCACATGAAG+TGG 0.499492 5:+19798683 None:intergenic
TATTATTTCGTCAAACTCAC+TGG 0.507205 5:+19798546 None:intergenic
CGCCAGTGAATGAAGCTTGG+CGG 0.508090 5:+19800059 None:intergenic
GCCAGTGAATGAAGCTTGGC+GGG 0.509193 5:+19800060 None:intergenic
AAGCGGAAGAATCAGCTGTT+GGG 0.517511 5:-19798612 MsG0580025510.01.T01:CDS
GAATGAAGCTTGGCGGGAGA+GGG 0.521311 5:+19800066 None:intergenic
GAGTTGAACCGGAGTGGACC+GGG 0.521646 5:+19800095 None:intergenic
GTGGGAAGCGGAGGAAGAGG+AGG 0.525243 5:-19798674 MsG0580025510.01.T01:CDS
AGAACTTCTCTAATGCGAAC+CGG 0.526010 5:-19800136 MsG0580025510.01.T01:CDS
AATGCGAACCGGTTCCGTCC+CGG 0.526633 5:-19800125 MsG0580025510.01.T01:CDS
AGAGGAGGAGGATGAAGTGG+TGG 0.531822 5:-19798659 MsG0580025510.01.T01:CDS
TGCCCACTTCATGTGGGAAG+CGG 0.532394 5:-19798686 MsG0580025510.01.T01:CDS
AAAGCGGAAGAATCAGCTGT+TGG 0.536393 5:-19798613 MsG0580025510.01.T01:CDS
ATACATATGAGTATCTAAAA+GGG 0.541772 5:-19798078 MsG0580025510.01.T01:three_prime_UTR
CATGTGGGAAGCGGAGGAAG+AGG 0.547034 5:-19798677 MsG0580025510.01.T01:CDS
AGAAGTAACATTATGATCTC+CGG 0.547577 5:+19798415 None:intergenic
GAGGATACCGGATTCGGCGG+AGG 0.548110 5:-19799863 MsG0580025510.01.T01:CDS
GGTGGTGAAAGGATGAAAAT+CGG 0.548568 5:-19799794 MsG0580025510.01.T01:CDS
TCTCTCTTCAATTATAGGGT+AGG 0.549352 5:+19798236 None:intergenic
GAAAAGCTGAAATTCTTGAT+CGG 0.563410 5:+19799926 None:intergenic
TAAAGATCTGTTGATTCCGT+CGG 0.563817 5:+19799973 None:intergenic
TTCAGAATGTCAAATGTGAA+AGG 0.567362 5:+19798372 None:intergenic
CTCCGCTTCCCACATGAAGT+GGG 0.568198 5:+19798684 None:intergenic
TGAAGCTTGGCGGGAGAGGG+AGG 0.572384 5:+19800069 None:intergenic
CGGAATGTTTGTCGGTGGAG+TGG 0.574481 5:+19799993 None:intergenic
GATCCTATGCCCACTTCATG+TGG 0.575020 5:-19798693 MsG0580025510.01.T01:CDS
GGTTAACGGCGGTGAAATGA+AGG 0.575859 5:-19798638 MsG0580025510.01.T01:CDS
TGTCGGTGGAGTGGAAATGC+AGG 0.576329 5:+19800002 None:intergenic
CTCAGCAAAAGCGCGTCGGT+AGG 0.580945 5:+19799740 None:intergenic
CTAATTGCGACGAATGTGAA+AGG 0.582249 5:-19798328 MsG0580025510.01.T01:three_prime_UTR
CCGGTGGAAACGGTGGTGAA+AGG 0.583383 5:-19799805 MsG0580025510.01.T01:CDS
CCACTTCATGTGGGAAGCGG+AGG 0.584056 5:-19798683 MsG0580025510.01.T01:CDS
TCCGTCGGAATGTTTGTCGG+TGG 0.584409 5:+19799988 None:intergenic
AGATCAGAAACAAAACAAGT+AGG 0.591950 5:+19798182 None:intergenic
TTCCCACATGAAGTGGGCAT+AGG 0.605173 5:+19798690 None:intergenic
ATCCTATGCCCACTTCATGT+GGG 0.612131 5:-19798692 MsG0580025510.01.T01:CDS
GTTCTAAACCCGGTCCACTC+CGG 0.618867 5:-19800103 MsG0580025510.01.T01:CDS
ATTTCTCAGCAAAAGCGCGT+CGG 0.621203 5:+19799736 None:intergenic
AGTGGACCGGGTTTAGAACC+GGG 0.630183 5:+19800107 None:intergenic
TGTCGCCAGTGAATGAAGCT+TGG 0.631457 5:+19800056 None:intergenic
GTCGGTGGAGTGGAAATGCA+GGG 0.643205 5:+19800003 None:intergenic
GTCAACACCGGTGGAAACGG+TGG 0.643860 5:-19799812 MsG0580025510.01.T01:CDS
GGAGGAGGCGGATTCGATCA+CGG 0.644648 5:-19799845 MsG0580025510.01.T01:CDS
GACCGGGTTTAGAACCGGGA+CGG 0.654441 5:+19800111 None:intergenic
GTTGAGGATACCGGATTCGG+CGG 0.656017 5:-19799866 MsG0580025510.01.T01:CDS
CGGTGAAATGAAGGTGAAAG+CGG 0.662249 5:-19798629 MsG0580025510.01.T01:CDS
AGGTTCGAGTTGAACCGGAG+TGG 0.664029 5:+19800089 None:intergenic
TCCACCGACAAACATTCCGA+CGG 0.679368 5:-19799989 MsG0580025510.01.T01:CDS
GATGTAACAGTCAACACCGG+TGG 0.692588 5:-19799821 MsG0580025510.01.T01:CDS
TTACGGGAAATATCTTCACG+AGG 0.714068 5:-19799714 MsG0580025510.01.T01:intron
GATGAAGTGGTGGTTAACGG+CGG 0.725938 5:-19798649 MsG0580025510.01.T01:CDS
GGTGATGTAACAGTCAACAC+CGG 0.748533 5:-19799824 MsG0580025510.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCTCTAATAATTTTTATT+TGG - Chr5:19799410-19799429 MsG0580025510.01.T01:intron 10.0%
!!! TTTGAATTATAACTATTTTA+AGG + Chr5:19800178-19800197 None:intergenic 10.0%
!! TGATCATTAATTACAATTAA+TGG + Chr5:19798758-19798777 None:intergenic 15.0%
!! TTGTGTAAAATTATTTACAT+TGG - Chr5:19799350-19799369 MsG0580025510.01.T01:intron 15.0%
!! AATACATATGAGTATCTAAA+AGG - Chr5:19800073-19800092 MsG0580025510.01.T01:CDS 20.0%
!! AATTAATGATCATGATAGTT+TGG - Chr5:19798766-19798785 MsG0580025510.01.T01:intron 20.0%
!! ATACATATGAGTATCTAAAA+GGG - Chr5:19800074-19800093 MsG0580025510.01.T01:CDS 20.0%
!! TAATGATTATGATTAAGTGA+TGG + Chr5:19799247-19799266 None:intergenic 20.0%
!!! AATATCAATTCACTGTATTT+TGG - Chr5:19799579-19799598 MsG0580025510.01.T01:CDS 20.0%
! AATAACAAAGTCTACATTCA+GGG + Chr5:19798933-19798952 None:intergenic 25.0%
! AATGTATAATACCTATCATC+AGG + Chr5:19798712-19798731 None:intergenic 25.0%
! ATAACGACAATAAATCTTTG+GGG + Chr5:19799127-19799146 None:intergenic 25.0%
! GATAACGACAATAAATCTTT+GGG + Chr5:19799128-19799147 None:intergenic 25.0%
! TAGTTATAATTCAAAGTTCC+AGG - Chr5:19800183-19800202 MsG0580025510.01.T01:five_prime_UTR 25.0%
! TTGCAATTTATCTACATTCA+GGG + Chr5:19798904-19798923 None:intergenic 25.0%
! TTTGCAATTTATCTACATTC+AGG + Chr5:19798905-19798924 None:intergenic 25.0%
!! ATGTGTGTGTAAATATTTTC+CGG - Chr5:19799211-19799230 MsG0580025510.01.T01:intron 25.0%
!! CACTTCTTTCTTTCTATTTT+CGG + Chr5:19798501-19798520 None:intergenic 25.0%
!! TGATAACGACAATAAATCTT+TGG + Chr5:19799129-19799148 None:intergenic 25.0%
!!! AGTTTATTTTGTAGAAAGAG+TGG + Chr5:19797993-19798012 None:intergenic 25.0%
!!! ATTTGGATTTTGATTTTTGC+AGG - Chr5:19798525-19798544 MsG0580025510.01.T01:three_prime_UTR 25.0%
AAAGAAAGAAGTGAGTGATT+TGG - Chr5:19798508-19798527 MsG0580025510.01.T01:three_prime_UTR 30.0%
ACTAATGAAGTTTGCATTCA+GGG + Chr5:19798965-19798984 None:intergenic 30.0%
AGAAGTAACATTATGATCTC+CGG + Chr5:19799740-19799759 None:intergenic 30.0%
AGATCAGAAACAAAACAAGT+AGG + Chr5:19799973-19799992 None:intergenic 30.0%
AGTCCAAATGTTTACTTAAC+CGG - Chr5:19799718-19799737 MsG0580025510.01.T01:CDS 30.0%
AGTCTCTCTCTTCAATTATA+GGG + Chr5:19799923-19799942 None:intergenic 30.0%
CACAATTGACAAATCACAAT+CGG + Chr5:19799335-19799354 None:intergenic 30.0%
CATCTATCAAAAGATTTACG+GGG - Chr5:19798831-19798850 MsG0580025510.01.T01:intron 30.0%
CCATCTATCAAAAGATTTAC+GGG - Chr5:19798830-19798849 MsG0580025510.01.T01:intron 30.0%
CCCATCTATCAAAAGATTTA+CGG - Chr5:19798829-19798848 MsG0580025510.01.T01:intron 30.0%
GAAAAGCTGAAATTCTTGAT+CGG + Chr5:19798229-19798248 None:intergenic 30.0%
GAATAACAAAGTCTACATTC+AGG + Chr5:19798934-19798953 None:intergenic 30.0%
GGTTATGTGACAACAATAAA+TGG - Chr5:19799845-19799864 MsG0580025510.01.T01:CDS 30.0%
TATTATTTCGTCAAACTCAC+TGG + Chr5:19799609-19799628 None:intergenic 30.0%
TCTCAGAATCTAAATCGAAT+CGG - Chr5:19798247-19798266 MsG0580025510.01.T01:three_prime_UTR 30.0%
TTCAGAATGTCAAATGTGAA+AGG + Chr5:19799783-19799802 None:intergenic 30.0%
TTGCAATTTCTCTTCATTCA+GGG - Chr5:19799054-19799073 MsG0580025510.01.T01:intron 30.0%
TTTGCAATTTCTCTTCATTC+AGG - Chr5:19799053-19799072 MsG0580025510.01.T01:intron 30.0%
TTTGCAGGTAGAGAAAAATA+TGG - Chr5:19798540-19798559 MsG0580025510.01.T01:three_prime_UTR 30.0%
! CCGTAAATCTTTTGATAGAT+GGG + Chr5:19798832-19798851 None:intergenic 30.0%
! TCTCTTTTCCAACGATTTTA+GGG - Chr5:19800114-19800133 MsG0580025510.01.T01:CDS 30.0%
! TTCTCTTTTCCAACGATTTT+AGG - Chr5:19800113-19800132 MsG0580025510.01.T01:CDS 30.0%
!!! TTGTTTTGTTTCTGATCTAC+TGG - Chr5:19799975-19799994 MsG0580025510.01.T01:CDS 30.0%
AAGTGATGGTTATGTTTAGC+CGG + Chr5:19799233-19799252 None:intergenic 35.0%
AATCACTTTAGTCCCTGAAT+GGG + Chr5:19799100-19799119 None:intergenic 35.0%
CACATTTGACATTCTGAACT+CGG - Chr5:19799785-19799804 MsG0580025510.01.T01:CDS 35.0%
CAGTCTCTCTCTTCAATTAT+AGG + Chr5:19799924-19799943 None:intergenic 35.0%
CTATTCATCCCTAAAATCGT+TGG + Chr5:19800125-19800144 None:intergenic 35.0%
CTCTCTTCAATTATAGGGTA+GGG + Chr5:19799918-19799937 None:intergenic 35.0%
GACTAATGAAGTTTGCATTC+AGG + Chr5:19798966-19798985 None:intergenic 35.0%
TAATCACTTTAGTCCCTGAA+TGG + Chr5:19799101-19799120 None:intergenic 35.0%
TCTCCGGTTAAGTAAACATT+TGG + Chr5:19799724-19799743 None:intergenic 35.0%
TCTCTCTTCAATTATAGGGT+AGG + Chr5:19799919-19799938 None:intergenic 35.0%
! AATCGGATCTCAATCTTTTC+CGG - Chr5:19798264-19798283 MsG0580025510.01.T01:three_prime_UTR 35.0%
! AATCGTTTTACACATGTTCG+TGG - Chr5:19799276-19799295 MsG0580025510.01.T01:intron 35.0%
! ATCGTTTTACACATGTTCGT+GGG - Chr5:19799277-19799296 MsG0580025510.01.T01:intron 35.0%
! CCCGTAAATCTTTTGATAGA+TGG + Chr5:19798833-19798852 None:intergenic 35.0%
! TGGTTTTCTAATATGCAGCA+CGG - Chr5:19799430-19799449 MsG0580025510.01.T01:intron 35.0%
!! AAACTTTTGGTGATTGAACG+CGG + Chr5:19798129-19798148 None:intergenic 35.0%
!! ATTTTAGCGAATCCTGAAGA+TGG - Chr5:19798592-19798611 MsG0580025510.01.T01:exon 35.0%
!! GCTTTACGGAAAGTTGATAT+GGG - Chr5:19798627-19798646 MsG0580025510.01.T01:CDS 35.0%
!! GGATTTAACTTGTGAATGCA+TGG - Chr5:19798787-19798806 MsG0580025510.01.T01:intron 35.0%
!! TAAAGATCTGTTGATTCCGT+CGG + Chr5:19798182-19798201 None:intergenic 35.0%
!! TTAACTTGTGAATGCATGGA+CGG - Chr5:19798791-19798810 MsG0580025510.01.T01:intron 35.0%
ACTAACGGAGTCTACATTCA+GGG + Chr5:19798997-19799016 None:intergenic 40.0%
ACTTTAGTCCCTGAATGGAA+AGG - Chr5:19798862-19798881 MsG0580025510.01.T01:intron 40.0%
AGAACTTCTCTAATGCGAAC+CGG - Chr5:19798016-19798035 MsG0580025510.01.T01:three_prime_UTR 40.0%
CTAATTGCGACGAATGTGAA+AGG - Chr5:19799824-19799843 MsG0580025510.01.T01:CDS 40.0%
CTTTAGTCCCTGAATGGAAA+GGG - Chr5:19798863-19798882 MsG0580025510.01.T01:intron 40.0%
GAAAAATATGGTGAGAGCAG+AGG - Chr5:19798552-19798571 MsG0580025510.01.T01:three_prime_UTR 40.0%
GAAAGCAATTCACCATCTTC+AGG + Chr5:19798607-19798626 None:intergenic 40.0%
GGTGAATTGCTTTCGCTTTA+CGG - Chr5:19798613-19798632 MsG0580025510.01.T01:CDS 40.0%
GGTGGTGAAAGGATGAAAAT+CGG - Chr5:19798358-19798377 MsG0580025510.01.T01:three_prime_UTR 40.0%
GTGAGAGCAGAGGAATATTA+TGG - Chr5:19798562-19798581 MsG0580025510.01.T01:three_prime_UTR 40.0%
TCAGGGACTAAAGTGACTAA+CGG + Chr5:19798856-19798875 None:intergenic 40.0%
TTACGGGAAATATCTTCACG+AGG - Chr5:19798438-19798457 MsG0580025510.01.T01:three_prime_UTR 40.0%
TTCATGTTGCTCCTGATGAT+AGG - Chr5:19798698-19798717 MsG0580025510.01.T01:CDS 40.0%
! ATCTCAATCTTTTCCGGTTG+AGG - Chr5:19798270-19798289 MsG0580025510.01.T01:three_prime_UTR 40.0%
! CGCTTTTGCTGAGAAATTAC+GGG - Chr5:19798422-19798441 MsG0580025510.01.T01:three_prime_UTR 40.0%
! GCGCTTTTGCTGAGAAATTA+CGG - Chr5:19798421-19798440 MsG0580025510.01.T01:three_prime_UTR 40.0%
! TAGTCACTTTAGTCCCTGAA+TGG - Chr5:19798857-19798876 MsG0580025510.01.T01:intron 40.0%
! TGGAAATGCAGGGAAACTTT+TGG + Chr5:19798142-19798161 None:intergenic 40.0%
!! CGCTTTACGGAAAGTTGATA+TGG - Chr5:19798626-19798645 MsG0580025510.01.T01:CDS 40.0%
AAAGCGGAAGAATCAGCTGT+TGG - Chr5:19799539-19799558 MsG0580025510.01.T01:CDS 45.0%
AAGCGGAAGAATCAGCTGTT+GGG - Chr5:19799540-19799559 MsG0580025510.01.T01:CDS 45.0%
ATCCTATGCCCACTTCATGT+GGG - Chr5:19799460-19799479 MsG0580025510.01.T01:CDS 45.0%
CGGTGAAATGAAGGTGAAAG+CGG - Chr5:19799523-19799542 MsG0580025510.01.T01:CDS 45.0%
CTAATATGCAGCACGGTGTT+GGG - Chr5:19799437-19799456 MsG0580025510.01.T01:intron 45.0%
GACGAATCCCTTTCCATTCA+GGG + Chr5:19798873-19798892 None:intergenic 45.0%
GACTAACGGAGTCTACATTC+AGG + Chr5:19798998-19799017 None:intergenic 45.0%
GAGGATGAAGTGGTGGTTAA+CGG - Chr5:19799500-19799519 MsG0580025510.01.T01:CDS 45.0%
GATTCCGTCGGAATGTTTGT+CGG + Chr5:19798170-19798189 None:intergenic 45.0%
GGTGATGTAACAGTCAACAC+CGG - Chr5:19798328-19798347 MsG0580025510.01.T01:three_prime_UTR 45.0%
TCCCTGAAATTGACCATGCA+GGG - Chr5:19799025-19799044 MsG0580025510.01.T01:intron 45.0%
TCCCTGCATGGTCAATTTCA+GGG + Chr5:19799029-19799048 None:intergenic 45.0%
TCTAATATGCAGCACGGTGT+TGG - Chr5:19799436-19799455 MsG0580025510.01.T01:intron 45.0%
TGACGAATCCCTTTCCATTC+AGG + Chr5:19798874-19798893 None:intergenic 45.0%
! ATTATAGGGTAGGGCGTTTC+TGG + Chr5:19799909-19799928 None:intergenic 45.0%
! ATTTCTCAGCAAAAGCGCGT+CGG + Chr5:19798419-19798438 None:intergenic 45.0%
! TCTTTTCCGGTTGAGGATAC+CGG - Chr5:19798277-19798296 MsG0580025510.01.T01:three_prime_UTR 45.0%
ACAGTCAACACCGGTGGAAA+CGG - Chr5:19798337-19798356 MsG0580025510.01.T01:three_prime_UTR 50.0%
ATTGCAAAGCAGTCCCTGCA+TGG + Chr5:19799041-19799060 None:intergenic 50.0%
CTTTAGTCCCTGAATGGGAG+AGG + Chr5:19799095-19799114 None:intergenic 50.0%
GACGAATCCTCTCCCATTCA+GGG - Chr5:19799085-19799104 MsG0580025510.01.T01:intron 50.0%
GATCCTATGCCCACTTCATG+TGG - Chr5:19799459-19799478 MsG0580025510.01.T01:CDS 50.0%
GATGAAGTGGTGGTTAACGG+CGG - Chr5:19799503-19799522 MsG0580025510.01.T01:CDS 50.0%
GATGTAACAGTCAACACCGG+TGG - Chr5:19798331-19798350 MsG0580025510.01.T01:three_prime_UTR 50.0%
GGTTAACGGCGGTGAAATGA+AGG - Chr5:19799514-19799533 MsG0580025510.01.T01:CDS 50.0%
GTCCCTGAAATTGACCATGC+AGG - Chr5:19799024-19799043 MsG0580025510.01.T01:intron 50.0%
GTCCCTGCATGGTCAATTTC+AGG + Chr5:19799030-19799049 None:intergenic 50.0%
TCCACCGACAAACATTCCGA+CGG - Chr5:19798163-19798182 MsG0580025510.01.T01:three_prime_UTR 50.0%
TGACGAATCCTCTCCCATTC+AGG - Chr5:19799084-19799103 MsG0580025510.01.T01:intron 50.0%
TGTCGCCAGTGAATGAAGCT+TGG + Chr5:19798099-19798118 None:intergenic 50.0%
TTCCCACATGAAGTGGGCAT+AGG + Chr5:19799465-19799484 None:intergenic 50.0%
!! CTTTTGGTGATTGAACGCGG+TGG + Chr5:19798126-19798145 None:intergenic 50.0%
AGAGGAGGAGGATGAAGTGG+TGG - Chr5:19799493-19799512 MsG0580025510.01.T01:CDS 55.0%
CCGAATCCGGTATCCTCAAC+CGG + Chr5:19798286-19798305 None:intergenic 55.0%
CCGGTTGAGGATACCGGATT+CGG - Chr5:19798283-19798302 MsG0580025510.01.T01:three_prime_UTR 55.0%
CCTTTCACCACCGTTTCCAC+CGG + Chr5:19798350-19798369 None:intergenic 55.0%
CGCCAGTGAATGAAGCTTGG+CGG + Chr5:19798096-19798115 None:intergenic 55.0%
CGGAATGTTTGTCGGTGGAG+TGG + Chr5:19798162-19798181 None:intergenic 55.0%
CTCCGCTTCCCACATGAAGT+GGG + Chr5:19799471-19799490 None:intergenic 55.0%
GAATGAAGCTTGGCGGGAGA+GGG + Chr5:19798089-19798108 None:intergenic 55.0%
GCCAGTGAATGAAGCTTGGC+GGG + Chr5:19798095-19798114 None:intergenic 55.0%
GGAAGAGGAGGAGGATGAAG+TGG - Chr5:19799490-19799509 MsG0580025510.01.T01:CDS 55.0%
GTCGGTGGAGTGGAAATGCA+GGG + Chr5:19798152-19798171 None:intergenic 55.0%
GTTGAGGATACCGGATTCGG+CGG - Chr5:19798286-19798305 MsG0580025510.01.T01:three_prime_UTR 55.0%
TCCCGCCAAGCTTCATTCAC+TGG - Chr5:19798091-19798110 MsG0580025510.01.T01:three_prime_UTR 55.0%
TCCGTCGGAATGTTTGTCGG+TGG + Chr5:19798167-19798186 None:intergenic 55.0%
TGAATGAAGCTTGGCGGGAG+AGG + Chr5:19798090-19798109 None:intergenic 55.0%
TGCCCACTTCATGTGGGAAG+CGG - Chr5:19799466-19799485 MsG0580025510.01.T01:CDS 55.0%
TGTCGGTGGAGTGGAAATGC+AGG + Chr5:19798153-19798172 None:intergenic 55.0%
! AGTGGACCGGGTTTAGAACC+GGG + Chr5:19798048-19798067 None:intergenic 55.0%
! GAGTGGACCGGGTTTAGAAC+CGG + Chr5:19798049-19798068 None:intergenic 55.0%
! GTTCTAAACCCGGTCCACTC+CGG - Chr5:19798049-19798068 MsG0580025510.01.T01:three_prime_UTR 55.0%
! GTTTAGAACCGGGACGGAAC+CGG + Chr5:19798038-19798057 None:intergenic 55.0%
!! AGGTTCGAGTTGAACCGGAG+TGG + Chr5:19798066-19798085 None:intergenic 55.0%
!! GAGGGAGGTTCGAGTTGAAC+CGG + Chr5:19798071-19798090 None:intergenic 55.0%
!! TTCCGTCCCGGTTCTAAACC+CGG - Chr5:19798039-19798058 MsG0580025510.01.T01:three_prime_UTR 55.0%
CATGTGGGAAGCGGAGGAAG+AGG - Chr5:19799475-19799494 MsG0580025510.01.T01:CDS 60.0%
CCACTTCATGTGGGAAGCGG+AGG - Chr5:19799469-19799488 MsG0580025510.01.T01:CDS 60.0%
CCGGTGGAAACGGTGGTGAA+AGG - Chr5:19798347-19798366 MsG0580025510.01.T01:three_prime_UTR 60.0%
CCTCCGCTTCCCACATGAAG+TGG + Chr5:19799472-19799491 None:intergenic 60.0%
GAGTTGAACCGGAGTGGACC+GGG + Chr5:19798060-19798079 None:intergenic 60.0%
GTCAACACCGGTGGAAACGG+TGG - Chr5:19798340-19798359 MsG0580025510.01.T01:three_prime_UTR 60.0%
! AATGCGAACCGGTTCCGTCC+CGG - Chr5:19798027-19798046 MsG0580025510.01.T01:three_prime_UTR 60.0%
! CGAGTTGAACCGGAGTGGAC+CGG + Chr5:19798061-19798080 None:intergenic 60.0%
! CTCAGCAAAAGCGCGTCGGT+AGG + Chr5:19798415-19798434 None:intergenic 60.0%
! GACCGGGTTTAGAACCGGGA+CGG + Chr5:19798044-19798063 None:intergenic 60.0%
! GGAGGAGGCGGATTCGATCA+CGG - Chr5:19798307-19798326 MsG0580025510.01.T01:three_prime_UTR 60.0%
GAGGATACCGGATTCGGCGG+AGG - Chr5:19798289-19798308 MsG0580025510.01.T01:three_prime_UTR 65.0%
GATACCGGATTCGGCGGAGG+AGG - Chr5:19798292-19798311 MsG0580025510.01.T01:three_prime_UTR 65.0%
GGAAGCGGAGGAAGAGGAGG+AGG - Chr5:19799481-19799500 MsG0580025510.01.T01:CDS 65.0%
GTGGGAAGCGGAGGAAGAGG+AGG - Chr5:19799478-19799497 MsG0580025510.01.T01:CDS 65.0%
TGAAGCTTGGCGGGAGAGGG+AGG + Chr5:19798086-19798105 None:intergenic 65.0%
ACCGGATTCGGCGGAGGAGG+CGG - Chr5:19798295-19798314 MsG0580025510.01.T01:three_prime_UTR 70.0%
TCCGCCTCCTCCGCCGAATC+CGG + Chr5:19798299-19798318 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 19797970 19800204 19797970 ID=MsG0580025510.01;Name=MsG0580025510.01
Chr5 mRNA 19797970 19800204 19797970 ID=MsG0580025510.01.T01;Parent=MsG0580025510.01;Name=MsG0580025510.01.T01;_AED=0.45;_eAED=0.45;_QI=43|1|1|1|1|1|3|624|250
Chr5 exon 19797970 19798726 19797970 ID=MsG0580025510.01.T01:exon:8346;Parent=MsG0580025510.01.T01
Chr5 exon 19799455 19799627 19799455 ID=MsG0580025510.01.T01:exon:8345;Parent=MsG0580025510.01.T01
Chr5 exon 19799715 19800204 19799715 ID=MsG0580025510.01.T01:exon:8344;Parent=MsG0580025510.01.T01
Chr5 five_prime_UTR 19800162 19800204 19800162 ID=MsG0580025510.01.T01:five_prime_utr;Parent=MsG0580025510.01.T01
Chr5 CDS 19799715 19800161 19799715 ID=MsG0580025510.01.T01:cds;Parent=MsG0580025510.01.T01
Chr5 CDS 19799455 19799627 19799455 ID=MsG0580025510.01.T01:cds;Parent=MsG0580025510.01.T01
Chr5 CDS 19798594 19798726 19798594 ID=MsG0580025510.01.T01:cds;Parent=MsG0580025510.01.T01
Chr5 three_prime_UTR 19797970 19798593 19797970 ID=MsG0580025510.01.T01:three_prime_utr;Parent=MsG0580025510.01.T01
Gene Sequence

>MsG0580025510.01.T01

ATGAGAACTTCTCTAATGCGAACCGGTTCCGTCCCGGTTCTAAACCCGGTCCACTCCGGTTCAACTCGAACCTCCCTCTCCCGCCAAGCTTCATTCACTGGCGACAACAGCCACCGCGTTCAATCACCAAAAGTTTCCCTGCATTTCCACTCCACCGACAAACATTCCGACGGAATCAACAGATCTTTATCAGAAAGCTTGATGATTAACTCACCGATCAAGAATTTCAGCTTTTCTCAGAATCTAAATCGAATCGGATCTCAATCTTTTCCGGTTGAGGATACCGGATTCGGCGGAGGAGGCGGATTCGATCACGGTGATGTAACAGTCAACACCGGTGGAAACGGTGGTGAAAGGATGAAAATCGGTGCGTATTATGAAGAAATGTTGAAGTCAAATCCTACCGACGCGCTTTTGCTGAGAAATTACGGGAAATATCTTCACGAGGTAGAGAAAAATATGGTGAGAGCAGAGGAATATTATGGAAGAGCGATTTTAGCGAATCCTGAAGATGGTGAATTGCTTTCGCTTTACGGAAAGTTGATATGGGAAATGAGTAGAGATGAAGAAAGAGCTCAATCTTACTTTGATCAAGCAATTCATGTTGCTCCTGATGATAGCACGGTGTTGGGATCCTATGCCCACTTCATGTGGGAAGCGGAGGAAGAGGAGGAGGATGAAGTGGTGGTTAACGGCGGTGAAATGAAGGTGAAAGCGGAAGAATCAGCTGTTGGGCTGATCGCTGCTTTTTAA
Protein sequence

>MsG0580025510.01.T01

MRTSLMRTGSVPVLNPVHSGSTRTSLSRQASFTGDNSHRVQSPKVSLHFHSTDKHSDGINRSLSESLMINSPIKNFSFSQNLNRIGSQSFPVEDTGFGGGGGFDHGDVTVNTGGNGGERMKIGAYYEEMLKSNPTDALLLRNYGKYLHEVEKNMVRAEEYYGRAILANPEDGELLSLYGKLIWEMSRDEERAQSYFDQAIHVAPDDSTVLGSYAHFMWEAEEEEEDEVVVNGGEMKVKAEESAVGLIAAF*