Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006818.01.T01 | XP_003593740.1 | 93.103 | 377 | 24 | 2 | 1 | 375 | 1 | 377 | 0 | 708 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006818.01 | MsG0280006860.01 | 0.854892 | 9.092555e-62 | 2.061329e-58 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006818.01.T01 | MTR_2g015630 | 93.103 | 377 | 24 | 2 | 1 | 375 | 1 | 377 | 0.0 | 708 |
| MsG0280006818.01.T01 | MTR_2g015580 | 73.127 | 387 | 97 | 5 | 2 | 383 | 15 | 399 | 0.0 | 587 |
| MsG0280006818.01.T01 | MTR_2g015580 | 32.500 | 160 | 76 | 5 | 454 | 583 | 176 | 333 | 1.11e-13 | 73.6 |
| MsG0280006818.01.T01 | MTR_2g015590 | 54.891 | 368 | 155 | 4 | 4 | 368 | 9 | 368 | 1.10e-141 | 416 |
| MsG0280006818.01.T01 | MTR_2g015590 | 33.125 | 160 | 77 | 6 | 455 | 584 | 159 | 318 | 1.28e-14 | 76.3 |
| MsG0280006818.01.T01 | MTR_2g015590 | 82.857 | 35 | 6 | 0 | 372 | 406 | 234 | 268 | 1.63e-11 | 66.6 |
| MsG0280006818.01.T01 | MTR_2g015590 | 55.853 | 299 | 123 | 3 | 4 | 301 | 9 | 299 | 6.61e-111 | 336 |
| MsG0280006818.01.T01 | MTR_2g015590 | 82.857 | 35 | 6 | 0 | 372 | 406 | 234 | 268 | 7.77e-12 | 67.4 |
| MsG0280006818.01.T01 | MTR_2g015650 | 47.633 | 338 | 171 | 3 | 30 | 365 | 31 | 364 | 1.97e-107 | 328 |
| MsG0280006818.01.T01 | MTR_2g015650 | 56.173 | 162 | 38 | 4 | 454 | 584 | 158 | 317 | 1.15e-43 | 160 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006818.01.T01 | AT1G28590 | 50.614 | 407 | 175 | 7 | 1 | 387 | 1 | 401 | 7.08e-134 | 397 |
| MsG0280006818.01.T01 | AT1G28590 | 34.940 | 166 | 76 | 6 | 454 | 588 | 162 | 326 | 2.05e-14 | 75.9 |
| MsG0280006818.01.T01 | AT1G31550 | 52.661 | 357 | 156 | 6 | 19 | 368 | 22 | 372 | 7.34e-128 | 382 |
| MsG0280006818.01.T01 | AT1G31550 | 32.716 | 162 | 78 | 5 | 454 | 584 | 161 | 322 | 5.20e-13 | 71.6 |
| MsG0280006818.01.T01 | AT2G27360 | 49.485 | 388 | 183 | 6 | 1 | 380 | 1 | 383 | 1.90e-127 | 380 |
| MsG0280006818.01.T01 | AT2G27360 | 31.902 | 163 | 76 | 7 | 454 | 583 | 160 | 320 | 3.11e-11 | 66.2 |
| MsG0280006818.01.T01 | AT1G28610 | 51.261 | 357 | 161 | 6 | 19 | 368 | 17 | 367 | 7.92e-127 | 379 |
| MsG0280006818.01.T01 | AT1G28610 | 33.540 | 161 | 75 | 6 | 454 | 583 | 157 | 316 | 1.17e-12 | 70.5 |
| MsG0280006818.01.T01 | AT1G28600 | 48.312 | 385 | 185 | 7 | 8 | 384 | 6 | 384 | 1.86e-126 | 378 |
| MsG0280006818.01.T01 | AT1G28580 | 50.270 | 370 | 168 | 5 | 10 | 368 | 10 | 374 | 3.05e-126 | 377 |
Find 151 sgRNAs with CRISPR-Local
Find 446 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGTCACTCATTGTGGT+GGG | 0.602896 | 2:+6664195 | MsG0280006818.01.T01:CDS |
| AAACACAGGTTACTAAATTG+AGG | 0.482807 | 2:-6662328 | None:intergenic |
| AAAGAAACTAATGTCCAAAG+CGG | 0.675281 | 2:-6658241 | None:intergenic |
| AAAGATATGAGTGTAAAGGA+GGG | 0.582514 | 2:+6658192 | MsG0280006818.01.T01:CDS |
| AACACAGGTTACTAAATTGA+GGG | 0.527776 | 2:-6662327 | None:intergenic |
| AACCCCTACAGAATAATTGG+TGG | 0.598978 | 2:-6658289 | None:intergenic |
| AAGAACCAATATGACTCATT+TGG | 0.406664 | 2:+6661821 | MsG0280006818.01.T01:CDS |
| AAGAATGCAGGTTAGACTGG+TGG | 0.604391 | 2:-6665103 | None:intergenic |
| AAGATAAATTTAGAAGCTCA+AGG | 0.536272 | 2:+6665174 | MsG0280006818.01.T01:three_prime_UTR |
| AAGTACGTTTCTCCGTAGGG+AGG | 0.709415 | 2:-6657969 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTACCTTCATGCTTAG+GGG | - | Chr2:6660863-6660882 | None:intergenic | 30.0% | |
| AAACACAGGTTACTAAATTG+AGG | - | Chr2:6662331-6662350 | None:intergenic | 30.0% | |
| AAAGAAACTAATGTCCAAAG+CGG | - | Chr2:6658244-6658263 | None:intergenic | 30.0% | |
| AAAGATATGAGTGTAAAGGA+GGG | + | Chr2:6658192-6658211 | MsG0280006818.01.T01:CDS | 30.0% | |
| AACACAGGTTACTAAATTGA+GGG | - | Chr2:6662330-6662349 | None:intergenic | 30.0% | |
| AAGAACCAATATGACTCATT+TGG | + | Chr2:6661821-6661840 | MsG0280006818.01.T01:CDS | 30.0% | |
| AAGACACACAAAAAACATTG+AGG | + | Chr2:6659038-6659057 | MsG0280006818.01.T01:intron | 30.0% | |
| AATTTAGTAACCTGTGTTTC+AGG | + | Chr2:6662332-6662351 | MsG0280006818.01.T01:intron | 30.0% | |
| ACAAACGTGCATTATGAAAT+TGG | - | Chr2:6658786-6658805 | None:intergenic | 30.0% | |
| ACATATAGATTAATTGCCGA+TGG | + | Chr2:6662280-6662299 | MsG0280006818.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 6657798 | 6665239 | 6657798 | ID=MsG0280006818.01;Name=MsG0280006818.01 |
| Chr2 | mRNA | 6657798 | 6665239 | 6657798 | ID=MsG0280006818.01.T01;Parent=MsG0280006818.01;Name=MsG0280006818.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|10|193|610 |
| Chr2 | exon | 6657798 | 6658044 | 6657798 | ID=MsG0280006818.01.T01:exon:2802;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6658133 | 6658363 | 6658133 | ID=MsG0280006818.01.T01:exon:2803;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6661428 | 6661576 | 6661428 | ID=MsG0280006818.01.T01:exon:2804;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6661725 | 6661998 | 6661725 | ID=MsG0280006818.01.T01:exon:2805;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6662137 | 6662337 | 6662137 | ID=MsG0280006818.01.T01:exon:2806;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6663217 | 6663334 | 6663217 | ID=MsG0280006818.01.T01:exon:2807;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6663499 | 6663638 | 6663499 | ID=MsG0280006818.01.T01:exon:2808;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6664178 | 6664283 | 6664178 | ID=MsG0280006818.01.T01:exon:2809;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6664454 | 6664680 | 6664454 | ID=MsG0280006818.01.T01:exon:2810;Parent=MsG0280006818.01.T01 |
| Chr2 | exon | 6664907 | 6665239 | 6664907 | ID=MsG0280006818.01.T01:exon:2811;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6657798 | 6658044 | 6657798 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6658133 | 6658363 | 6658133 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6661428 | 6661576 | 6661428 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6661725 | 6661998 | 6661725 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6662137 | 6662337 | 6662137 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6663217 | 6663334 | 6663217 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6663499 | 6663638 | 6663499 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6664178 | 6664283 | 6664178 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6664454 | 6664680 | 6664454 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | CDS | 6664907 | 6665046 | 6664907 | ID=MsG0280006818.01.T01:cds;Parent=MsG0280006818.01.T01 |
| Chr2 | three_prime_UTR | 6665047 | 6665239 | 6665047 | ID=MsG0280006818.01.T01:three_prime_utr;Parent=MsG0280006818.01.T01 |
| Gene Sequence |
>MsG0280006818.01.T01 ATGGCTTCTGAAAAGCGATGGAGTACAATGATGCTGCTTGTAGTAATGGCTTCTGCATCATTAGTCATGGCATCCTCTTCCTGCTCTTCCATCTTCAGCTTCGGTGATTCCCTCGCCGACAACGGGAACTTGTATTTCAGCAACCAGCAACCCTACCATCACTGCCTCTTTCCTCCCTACGGAGAAACGTACTTTCATCATCCCTCAGCACGTTGCTCTGATGGACGTCTCATCATTGATTTCATAGCTGAGGCATTGGGAATTCAAATGGTGAAACCATATTTAGGAATTAAGAATGATGTATTGAAAGATATGAGTGTAAAGGAGGGAGTGAATTTTGCTGTTATGGGAGCAACCGCTTTGGACATTAGTTTCTTTGAAGAAAGAGGTGTTCATAGTGTTACCACCAATTATTCTGTAGGGGTTCAGTTGAATTGGTTCAAGGAACTACTCCCTCGTCTCTGCAATTCTTCCAAAACCTGCCATGAAGTTCTTGGAAAGTCATTATTTCTTGTGGGAGAAATTGGAGGCAACGACTTCAATTATCCTTTACATATACGACAGAGCATAACAAAGCTTAAAGCATATGTACCACATGTAATCAATGCAATAACTTTAGCAATTAACGAGTTGATTGATCTAGGGGCACGCACGCTTATGGTCCCTGGAAATTTCCCATTGGGATGTAGTGCAGCCCATTTAACAACGTATGAAACCACAGATAAGAACCAATATGACTCATTTGGTTGCTTGAAGTGGTTAAACGAATTTGCTGAATTTTATAACCAGAAACTTCAACATGAGATACATAGACTTCGAGTAATTCATCCTCATGCTAATATCATCTATGCTGATTATTACAATGCAGCATTGCCATTATATCGTTATCCCAAAAAATATGGTTTTACAGGTCTCAAAGCATGTTGTGGAATAGGAAGTCCATATAATTACAATGCATCAAACATGTGTGGTAAACCAGGCGTGACTGTTTGTGATGATCCTTCTCAGTATATTACGTGGGATGGCGTTCATTTTACTGAGGCCACATATAGATTAATTGCCGATGGCTTAATTAAAGGACCATGTTCAGTCCCTCAATTTAATGTTTGCGACATGGATGCAACTGAAGATGAAGATGAGTATGATCAAGCTGGATGTTTGACATGGTTAAACAAGTTTTTTGAATATCATAATGAGCTGCTTCAAATCGAACTAAATAGCTCTTTAAGTGACACTGGCAATTTCCTTGCCACTGGTGCCAATTTATTCACAGCCATAGGTCAGTCTCCTTATGGTGAAGCCTTCTTTAGACATGCAACTGGTCTTTGCTCAGATGGACGACTAGTGATTGATTTCATAGATTGTGATAGCTACTTCAAAAGGTCACTCATTGTGGTGGGAGAGATAGGTGGTAATGATTATAACTATGCTGCCTTTGCTGGAGATGACTCTATCTTCAAGCCACCGAGCAGTGGAGCTTTTGGTGCCGGTAATTTACTTGTTGGATGCTCTGCTGTGTACCTAACTTTGTTAAGAAGCAATAACATAGAGGACTATGATGAAAATGGATGTTTAAAATTCAACGGTTTTGCAAAGTACCATAACATGGAGCTCAATATTGCCTTAGAGACACCCAGGAAAAAGAATCTTCATGCAACAAAACATGTGCATAATGAAAGACATGTGTCGCCTGGGTTCACTAATGGGGCTTTGAGAACGTGCTGTGGTGGAGGTGGACGTTACAATTTTAATAAATTCAGCAAGATGCGGTTAAGGGTTCAAAAGTATGTTCAGATCCATCTACATTTACAAATTGGGATGGAATTCACTTAA |
| Protein sequence |
>MsG0280006818.01.T01 MASEKRWSTMMLLVVMASASLVMASSSCSSIFSFGDSLADNGNLYFSNQQPYHHCLFPPYGETYFHHPSARCSDGRLIIDFIAEALGIQMVKPYLGIKNDVLKDMSVKEGVNFAVMGATALDISFFEERGVHSVTTNYSVGVQLNWFKELLPRLCNSSKTCHEVLGKSLFLVGEIGGNDFNYPLHIRQSITKLKAYVPHVINAITLAINELIDLGARTLMVPGNFPLGCSAAHLTTYETTDKNQYDSFGCLKWLNEFAEFYNQKLQHEIHRLRVIHPHANIIYADYYNAALPLYRYPKKYGFTGLKACCGIGSPYNYNASNMCGKPGVTVCDDPSQYITWDGVHFTEATYRLIADGLIKGPCSVPQFNVCDMDATEDEDEYDQAGCLTWLNKFFEYHNELLQIELNSSLSDTGNFLATGANLFTAIGQSPYGEAFFRHATGLCSDGRLVIDFIDCDSYFKRSLIVVGEIGGNDYNYAAFAGDDSIFKPPSSGAFGAGNLLVGCSAVYLTLLRSNNIEDYDENGCLKFNGFAKYHNMELNIALETPRKKNLHATKHVHNERHVSPGFTNGALRTCCGGGGRYNFNKFSKMRLRVQKYVQIHLHLQIGMEFT* |
