Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006856.01.T01 | XP_003593736.1 | 87.379 | 412 | 29 | 2 | 1 | 412 | 11 | 399 | 0 | 739 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006815.01 | MsG0280006856.01 | 0.871355 | 7.278307e-67 | 2.927114e-63 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006856.01.T01 | MTR_2g015580 | 87.379 | 412 | 29 | 2 | 1 | 412 | 11 | 399 | 0.0 | 739 |
| MsG0280006856.01.T01 | MTR_2g015630 | 73.479 | 411 | 81 | 5 | 5 | 412 | 2 | 387 | 0.0 | 589 |
| MsG0280006856.01.T01 | MTR_2g015590 | 53.015 | 398 | 149 | 6 | 7 | 400 | 9 | 372 | 5.37e-145 | 417 |
| MsG0280006856.01.T01 | MTR_2g015590 | 56.954 | 302 | 117 | 3 | 7 | 307 | 9 | 298 | 3.11e-119 | 350 |
| MsG0280006856.01.T01 | MTR_2g015650 | 45.902 | 366 | 161 | 9 | 35 | 393 | 29 | 364 | 6.27e-107 | 320 |
| MsG0280006856.01.T01 | MTR_3g463960 | 43.089 | 369 | 182 | 7 | 35 | 400 | 42 | 385 | 1.90e-97 | 296 |
| MsG0280006856.01.T01 | MTR_3g463950 | 43.089 | 369 | 182 | 7 | 35 | 400 | 42 | 385 | 1.90e-97 | 296 |
| MsG0280006856.01.T01 | MTR_3g463970 | 43.089 | 369 | 182 | 7 | 35 | 400 | 42 | 385 | 1.90e-97 | 296 |
| MsG0280006856.01.T01 | MTR_3g463940 | 43.089 | 369 | 182 | 7 | 35 | 400 | 42 | 385 | 1.90e-97 | 296 |
| MsG0280006856.01.T01 | MTR_3g448360 | 41.495 | 388 | 195 | 6 | 17 | 403 | 15 | 371 | 2.76e-95 | 290 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006856.01.T01 | AT1G28590 | 51.834 | 409 | 156 | 9 | 1 | 400 | 1 | 377 | 2.61e-137 | 399 |
| MsG0280006856.01.T01 | AT1G28580 | 49.752 | 404 | 171 | 6 | 1 | 399 | 1 | 377 | 9.73e-134 | 389 |
| MsG0280006856.01.T01 | AT1G28600 | 49.492 | 394 | 163 | 8 | 12 | 400 | 9 | 371 | 3.12e-132 | 385 |
| MsG0280006856.01.T01 | AT1G28610 | 50.639 | 391 | 157 | 8 | 15 | 400 | 12 | 371 | 3.80e-132 | 385 |
| MsG0280006856.01.T01 | AT2G27360 | 50.248 | 404 | 168 | 7 | 1 | 400 | 1 | 375 | 2.34e-131 | 383 |
| MsG0280006856.01.T01 | AT1G31550 | 50.127 | 395 | 153 | 9 | 15 | 400 | 17 | 376 | 2.59e-128 | 375 |
| MsG0280006856.01.T01 | AT1G28570 | 51.337 | 374 | 150 | 6 | 32 | 400 | 29 | 375 | 1.46e-126 | 371 |
| MsG0280006856.01.T01 | AT1G31550 | 49.618 | 393 | 155 | 8 | 15 | 400 | 17 | 373 | 1.48e-126 | 371 |
| MsG0280006856.01.T01 | AT1G28650 | 48.329 | 389 | 173 | 5 | 15 | 400 | 15 | 378 | 1.89e-121 | 358 |
| MsG0280006856.01.T01 | AT1G28670 | 48.974 | 390 | 170 | 6 | 15 | 400 | 13 | 377 | 5.04e-120 | 354 |
Find 103 sgRNAs with CRISPR-Local
Find 627 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCTGTTGCGGGAAGGG+AGG | 0.610533 | 2:+7132301 | MsG0280006856.01.T01:CDS |
| AAATAAGTTTGGCCGTAAGG+AGG | 0.579199 | 2:-7124069 | None:intergenic |
| AAATTTCTCATGGCGTCTGC+TGG | 0.459147 | 2:+7123868 | MsG0280006856.01.T01:exon |
| AACAGTGATCAGAGGGTGGT+TGG | 0.465343 | 2:-7124041 | None:intergenic |
| AAGAAACAGTGATCAGAGGG+TGG | 0.687519 | 2:-7124045 | None:intergenic |
| AAGCACAAGTTACTAAATTG+AGG | 0.529547 | 2:-7132475 | None:intergenic |
| AAGGGAGGTTCATATAATTT+CGG | 0.383941 | 2:+7132316 | MsG0280006856.01.T01:CDS |
| AATAAGTTTGGCCGTAAGGA+GGG | 0.568417 | 2:-7124068 | None:intergenic |
| AATGTTAACTTGTGCCTTAA+AGG | 0.282669 | 2:+7132670 | MsG0280006856.01.T01:three_prime_UTR |
| AATTTCGGATCAGAAACTTG+TGG | 0.661798 | 2:+7132331 | MsG0280006856.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATCAATATGACTCAGC+TGG | + | Chr2:7131833-7131852 | MsG0280006856.01.T01:CDS | 30.0% | |
| AAAGGGTAAAAAGAAAAGCA+AGG | - | Chr2:7129804-7129823 | None:intergenic | 30.0% | |
| AAATAAAACCTCAGCTATTG+AGG | + | Chr2:7125400-7125419 | MsG0280006856.01.T01:intron | 30.0% | |
| AACATTGCATTGTCATATGT+AGG | + | Chr2:7129145-7129164 | MsG0280006856.01.T01:intron | 30.0% | |
| AAGAAAAGGTCAATTATCCA+TGG | - | Chr2:7126206-7126225 | None:intergenic | 30.0% | |
| AAGCACAAGTTACTAAATTG+AGG | - | Chr2:7132478-7132497 | None:intergenic | 30.0% | |
| AAGGGAGGTTCATATAATTT+CGG | + | Chr2:7132316-7132335 | MsG0280006856.01.T01:CDS | 30.0% | |
| AATACAATAAACTAAGCACG+CGG | + | Chr2:7127969-7127988 | MsG0280006856.01.T01:intron | 30.0% | |
| AATTAATAATTGGTAGGGTC+TGG | - | Chr2:7124965-7124984 | None:intergenic | 30.0% | |
| ACTAGAAAGGTGAAACATAT+TGG | - | Chr2:7130215-7130234 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 7123856 | 7132771 | 7123856 | ID=MsG0280006856.01;Name=MsG0280006856.01 |
| Chr2 | mRNA | 7123856 | 7132771 | 7123856 | ID=MsG0280006856.01.T01;Parent=MsG0280006856.01;Name=MsG0280006856.01.T01;_AED=0.46;_eAED=0.48;_QI=21|0.6|0.83|1|0.8|0.5|6|234|412 |
| Chr2 | exon | 7123856 | 7124144 | 7123856 | ID=MsG0280006856.01.T01:exon:2063;Parent=MsG0280006856.01.T01 |
| Chr2 | exon | 7124243 | 7124473 | 7124243 | ID=MsG0280006856.01.T01:exon:2064;Parent=MsG0280006856.01.T01 |
| Chr2 | exon | 7131473 | 7131621 | 7131473 | ID=MsG0280006856.01.T01:exon:2065;Parent=MsG0280006856.01.T01 |
| Chr2 | exon | 7131737 | 7132010 | 7131737 | ID=MsG0280006856.01.T01:exon:2066;Parent=MsG0280006856.01.T01 |
| Chr2 | exon | 7132138 | 7132203 | 7132138 | ID=MsG0280006856.01.T01:exon:2067;Parent=MsG0280006856.01.T01 |
| Chr2 | exon | 7132287 | 7132771 | 7132287 | ID=MsG0280006856.01.T01:exon:2068;Parent=MsG0280006856.01.T01 |
| Chr2 | five_prime_UTR | 7123856 | 7123876 | 7123856 | ID=MsG0280006856.01.T01:five_prime_utr;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7123877 | 7124144 | 7123877 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7124243 | 7124473 | 7124243 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7131473 | 7131621 | 7131473 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7131737 | 7132010 | 7131737 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7132138 | 7132203 | 7132138 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | CDS | 7132287 | 7132537 | 7132287 | ID=MsG0280006856.01.T01:cds;Parent=MsG0280006856.01.T01 |
| Chr2 | three_prime_UTR | 7132538 | 7132771 | 7132538 | ID=MsG0280006856.01.T01:three_prime_utr;Parent=MsG0280006856.01.T01 |
| Gene Sequence |
>MsG0280006856.01.T01 ATGGCGTCTGCTGGTTCTGAACAGCGATGGAGCATTGTAACATTACAATTACTTCTTGTAATTACAGTTTCAGCACCGTTATTCACGGCGGCATGCTCCTCTTATTCTTCCATATTCAGCTTCGGCGATTCCATCGCCGACACTGGCAACCTGTATCTCATCTCCCAACCACCCTCTGATCACTGTTTCTTCCCTCCTTACGGCCAAACTTATTTTCATCATCCATCTGGACGTTGCTCTGATGGACGCCTCATCATTGATTTCATCGCGGAGTCATTGGGAATTCCAATGGTGAAACCATATTTAGGAATTAAGAATGGTGTATTTGAAGATAAGAGTGCAAAGGAAGGAGCAAATTTTGCTGTTATAGGAGCAACGGCGTTGGACGTTAGTTTCTTTGAAGAGAGGGGCGTTCTTAATTATAATAACAATTATTCTTTGTCAGTTCAACTGAATTGGTTCAAGGAATTTCTCCCTTCTCTTTGCAATTCCTCCAAAAACTGCCATGAAGTTCTTGGGAACTCATTATTTCTTATGGGAGAGATTGGAGGTAACGACTTCAATTATCCTTTATTTATACGAAGGAGCATAGCAGAGATTAAAACATACGTGCCACATGTAATCAATGCAATCACTTCAGCAATCAATGAGTTGATTGATCTAGGGGCACGCACACTCATGGTCCCTGGGAATTTCCCATTGGGATGCAATGCAATCTATTTAACAAAATATGAAACCACAGATAAAAATCAATATGACTCAGCTGGTTGCTTGAAATGGTTGAATGAATTTGCTGAATTTTATAATCAGGAGCTCCAATATGAGTTACATAGGCTAAGAAGAATTCATCCTCATGCTACTATCATTTATGCTGATTATTACAATGCTCTATTGCCATTATATCATAATCCCACAAAATTTGATCTAAAACCGGAAATGATAGTAAAGTCTACACTAGCTAGTTTAATTGAACTACTTTATTATCTAGGTTTTACAGGTCTCAAAAGCTGTTGCGGGAAGGGAGGTTCATATAATTTCGGATCAGAAACTTGTGGTAAACCAGGTGTGATTGCTTGTGATGATCCTTCTCAGTATATTGGATGGGATGGTGTTCATTTGACTGAGGCTGCTTATAGATTAATTGCTGATGGCATCATTAATGGACCATGTTCTGTTCCTCAATTTAGTAACTTGTGCTTCATGAATGTGAGCTATGAATATGGTCATTTCAATAGCTAG |
| Protein sequence |
>MsG0280006856.01.T01 MASAGSEQRWSIVTLQLLLVITVSAPLFTAACSSYSSIFSFGDSIADTGNLYLISQPPSDHCFFPPYGQTYFHHPSGRCSDGRLIIDFIAESLGIPMVKPYLGIKNGVFEDKSAKEGANFAVIGATALDVSFFEERGVLNYNNNYSLSVQLNWFKEFLPSLCNSSKNCHEVLGNSLFLMGEIGGNDFNYPLFIRRSIAEIKTYVPHVINAITSAINELIDLGARTLMVPGNFPLGCNAIYLTKYETTDKNQYDSAGCLKWLNEFAEFYNQELQYELHRLRRIHPHATIIYADYYNALLPLYHNPTKFDLKPEMIVKSTLASLIELLYYLGFTGLKSCCGKGGSYNFGSETCGKPGVIACDDPSQYIGWDGVHLTEAAYRLIADGIINGPCSVPQFSNLCFMNVSYEYGHFNS* |
