AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006864.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006864.01.T01 MTR_2g015690 96.860 414 13 0 20 433 1 414 0.0 827
MsG0280006864.01.T01 MTR_4g087810 62.374 396 141 4 38 431 12 401 1.03e-176 500
MsG0280006864.01.T01 MTR_4g087830 61.364 396 145 4 38 431 3 392 2.22e-174 494
MsG0280006864.01.T01 MTR_4g080100 47.044 406 180 5 43 423 32 427 3.90e-124 367
MsG0280006864.01.T01 MTR_4g077070 43.489 407 197 8 39 423 100 495 1.20e-105 323
MsG0280006864.01.T01 MTR_7g096120 39.723 433 233 6 14 425 49 474 1.71e-102 314
MsG0280006864.01.T01 MTR_7g096140 41.221 393 203 6 39 410 74 459 2.62e-96 297
MsG0280006864.01.T01 MTR_5g013230 34.123 422 215 12 5 388 99 495 1.26e-62 211
MsG0280006864.01.T01 MTR_7g081645 36.986 365 184 15 38 380 74 414 1.42e-57 195
MsG0280006864.01.T01 MTR_7g006400 35.143 350 179 10 39 366 105 428 8.48e-54 187
MsG0280006864.01.T01 MTR_7g081620 34.975 406 197 15 33 389 63 450 5.86e-52 181
MsG0280006864.01.T01 MTR_4g099510 33.071 381 197 9 38 388 128 480 1.10e-49 176
MsG0280006864.01.T01 MTR_1995s0010 39.732 224 110 5 165 367 1 220 6.00e-41 145
MsG0280006864.01.T01 MTR_2g020020 30.769 182 91 8 150 329 89 237 9.92e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006864.01.T01 AT4G18550 61.461 397 148 3 39 432 36 430 0.0 521
MsG0280006864.01.T01 AT4G18550 61.461 397 148 3 39 432 19 413 0.0 521
MsG0280006864.01.T01 AT1G06250 52.685 391 178 2 39 425 5 392 3.17e-151 436
MsG0280006864.01.T01 AT4G18550 61.290 279 105 2 39 316 36 312 5.83e-126 367
MsG0280006864.01.T01 AT2G42690 44.096 415 203 5 37 431 2 407 2.10e-112 337
MsG0280006864.01.T01 AT1G06800 42.476 412 199 10 39 423 85 485 3.08e-102 314
MsG0280006864.01.T01 AT1G51440 43.468 421 195 11 38 427 86 494 1.85e-100 310
MsG0280006864.01.T01 AT1G06250 51.601 281 129 2 149 425 1 278 1.84e-99 300
MsG0280006864.01.T01 AT2G30550 40.380 421 214 10 29 423 91 500 5.06e-97 301
MsG0280006864.01.T01 AT1G06800 41.274 361 174 10 39 372 85 434 1.38e-84 266
MsG0280006864.01.T01 AT1G30370 37.857 420 226 8 25 423 100 505 8.88e-84 266
MsG0280006864.01.T01 AT2G30550 39.566 369 186 10 29 371 91 448 6.90e-82 259
MsG0280006864.01.T01 AT2G44810 35.884 379 180 13 39 380 86 438 8.11e-60 202
MsG0280006864.01.T01 AT2G44810 36.193 373 175 13 45 380 2 348 2.62e-58 195
MsG0280006864.01.T01 AT4G16820 35.602 382 187 8 37 387 125 478 5.13e-58 199
MsG0280006864.01.T01 AT1G05800 34.699 366 198 9 31 376 72 416 2.61e-55 190
MsG0280006864.01.T01 AT2G31690 34.770 348 189 7 39 366 88 417 1.25e-54 189
MsG0280006864.01.T01 AT5G18640 32.927 164 83 6 168 329 100 238 5.14e-15 75.5
MsG0280006864.01.T01 AT5G18640 32.927 164 83 6 168 329 112 250 1.07e-14 75.9
MsG0280006864.01.T01 AT5G18640 32.927 164 83 6 168 329 112 250 1.07e-14 75.9
MsG0280006864.01.T01 AT5G18640 32.927 164 83 6 168 329 100 238 1.08e-14 75.5
MsG0280006864.01.T01 AT5G18640 32.927 164 83 6 168 329 100 238 1.08e-14 75.5

Find 99 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTATTGAAGGGAGATTT+TGG 0.169096 2:+7220770 None:intergenic
GTTTCTGCTCCTAAAGTATT+TGG 0.175913 2:-7220860 MsG0280006864.01.T01:CDS
ACATCTGCATACCCAATAAA+AGG 0.189119 2:+7219318 None:intergenic
ATGGAGGGTTGTAGAGAATA+AGG 0.217063 2:-7219085 MsG0280006864.01.T01:CDS
ACATAGATTTACCTGGTTTC+GGG 0.253359 2:+7220742 None:intergenic
AACATAGATTTACCTGGTTT+CGG 0.266015 2:+7220741 None:intergenic
TGGATCTTCAGAAGTATAAA+TGG 0.266518 2:+7220790 None:intergenic
TAATGATCTTCAGTTTCTTT+TGG 0.277450 2:-7220882 MsG0280006864.01.T01:CDS
CTGCTATTACCAAATACTTT+AGG 0.283364 2:+7220851 None:intergenic
TTTGTTACAGAATATTTAAA+AGG 0.323206 2:+7221097 None:intergenic
TACCAACCTTGATGCACTTT+AGG 0.338088 2:+7220815 None:intergenic
CTTGGAGTAAGGAATCAAAT+TGG 0.342591 2:-7221006 MsG0280006864.01.T01:CDS
TACCCAATAAAAGGGTAGTT+TGG 0.342990 2:+7219327 None:intergenic
TATAATTGAGAATTTATTCA+TGG 0.362934 2:-7218989 MsG0280006864.01.T01:three_prime_UTR
CGCTTCCGGAACATTGATTT+CGG 0.366404 2:+7221057 None:intergenic
TATGAACATTATTGTATGTT+TGG 0.369024 2:-7218826 MsG0280006864.01.T01:three_prime_UTR
ATTTACCTGGTTTCGGGCAC+TGG 0.370820 2:+7220748 None:intergenic
AGATGGTTCATGGAAGCTTA+TGG 0.372107 2:-7219046 MsG0280006864.01.T01:CDS
GCTATGCTGCTGTTGTTGTT+TGG 0.373108 2:+7221331 None:intergenic
GAAGGGATATTGTTATTGCT+TGG 0.374037 2:-7220934 MsG0280006864.01.T01:CDS
GAAATAAGCATAACTATAAC+AGG 0.376230 2:-7219561 MsG0280006864.01.T01:CDS
ACTATAACAGGTCACAGTTT+AGG 0.384060 2:-7219549 MsG0280006864.01.T01:CDS
CATAATCCACTATGGTGAAA+TGG 0.388866 2:-7221230 MsG0280006864.01.T01:CDS
CAAAGTTTGCAGGTAGTTGT+AGG 0.393065 2:-7221168 MsG0280006864.01.T01:CDS
TCGGGCACTGGTCTTATTGA+AGG 0.398715 2:+7220760 None:intergenic
AACTCAGCGGCGAAAATCAT+TGG 0.405365 2:-7221294 MsG0280006864.01.T01:CDS
TTGTATGTTTGGCCCTCCTT+TGG 0.405487 2:-7218815 MsG0280006864.01.T01:three_prime_UTR
GGAAAGATGTGTTGGGGAGA+AGG 0.411910 2:-7220952 MsG0280006864.01.T01:CDS
GACTTTATAATAAACGCTTC+CGG 0.415146 2:+7221043 None:intergenic
GTAAGGAATCAAATTGGATT+GGG 0.415930 2:-7221000 MsG0280006864.01.T01:CDS
AGTAAGGAATCAAATTGGAT+TGG 0.423060 2:-7221001 MsG0280006864.01.T01:CDS
GCTTATTTGCATGGAGTGGC+AGG 0.430370 2:-7219213 MsG0280006864.01.T01:CDS
TCTAGGGCTAGCAAATACTA+TGG 0.437648 2:+7219434 None:intergenic
GCAAATACTATGGCTGTAAC+TGG 0.439803 2:+7219444 None:intergenic
TTCAACAATCAGATGGTTCA+TGG 0.443571 2:-7219056 MsG0280006864.01.T01:CDS
AACGATACAAACTTTGGAAT+GGG 0.445770 2:-7220906 MsG0280006864.01.T01:CDS
GACTTCCGAAATCAATGTTC+CGG 0.446184 2:-7221062 MsG0280006864.01.T01:CDS
GAGAAAGCATCAAAGTTTGC+AGG 0.452694 2:-7221178 MsG0280006864.01.T01:CDS
ATCTAGAGAAGCTTGGAGTA+AGG 0.456251 2:-7221017 MsG0280006864.01.T01:CDS
CGACGTTACATAATCCACTA+TGG 0.458056 2:-7221238 MsG0280006864.01.T01:CDS
TATGAAGGTGAAGGCCTCTC+TGG 0.459413 2:-7221428 MsG0280006864.01.T01:CDS
AAGTTTGTGTCACCAACTCT+AGG 0.461380 2:+7219417 None:intergenic
GAAAGATGTGTTGGGGAGAA+GGG 0.463613 2:-7220951 MsG0280006864.01.T01:CDS
TTTGTACGTAGTATATATGA+AGG 0.465054 2:-7221443 MsG0280006864.01.T01:exon
GCAGTGGACATTGTTACAAA+TGG 0.466972 2:-7219504 MsG0280006864.01.T01:CDS
GAGAGGAACGATACAAACTT+TGG 0.468667 2:-7220912 MsG0280006864.01.T01:CDS
TCTATGTATTTGAAGAGTCC+AGG 0.484311 2:-7219264 MsG0280006864.01.T01:CDS
AATTTGGAGGCTTATTTGCA+TGG 0.495411 2:-7219222 MsG0280006864.01.T01:CDS
AGTCTTTATCTAGAGAAGCT+TGG 0.498976 2:-7221024 MsG0280006864.01.T01:CDS
TTTAAGTTGGCATAATTTGG+AGG 0.502108 2:-7219235 MsG0280006864.01.T01:CDS
TGCCAACTTAAAATATTCCC+TGG 0.503206 2:+7219246 None:intergenic
GATGAAGGGAAAGATGTGTT+GGG 0.510788 2:-7220959 MsG0280006864.01.T01:CDS
CTTGTGCCATTTCACCATAG+TGG 0.510988 2:+7221224 None:intergenic
GAGTAGCAATCATGATCAAA+AGG 0.511049 2:-7221381 MsG0280006864.01.T01:CDS
TTGGATTGGGTTTGTTGCTG+TGG 0.511970 2:-7220987 MsG0280006864.01.T01:CDS
CTTAAGTGAGGTGAGAAGGT+TGG 0.512596 2:-7219604 MsG0280006864.01.T01:CDS
AAGTGAGGTGAGAAGGTTGG+TGG 0.515539 2:-7219601 MsG0280006864.01.T01:CDS
TGGAGGGTTGTAGAGAATAA+GGG 0.516398 2:-7219084 MsG0280006864.01.T01:CDS
GAACGATACAAACTTTGGAA+TGG 0.518160 2:-7220907 MsG0280006864.01.T01:CDS
ACAGCAGCATAGCAAACAAA+TGG 0.523423 2:-7221321 MsG0280006864.01.T01:CDS
CGGGCACTGGTCTTATTGAA+GGG 0.526702 2:+7220761 None:intergenic
GGTTGTAGAGAATAAGGGTA+TGG 0.529061 2:-7219079 MsG0280006864.01.T01:CDS
CATCTGCATACCCAATAAAA+GGG 0.530668 2:+7219319 None:intergenic
GTTGCTGTGGCTAATGATGA+AGG 0.534220 2:-7220974 MsG0280006864.01.T01:CDS
TGATGAAGGGAAAGATGTGT+TGG 0.535789 2:-7220960 MsG0280006864.01.T01:CDS
TTTATTGGGTATGCAGATGT+TGG 0.539197 2:-7219315 MsG0280006864.01.T01:CDS
CATGGAGTGGCAGGAACACA+AGG 0.541108 2:-7219204 MsG0280006864.01.T01:CDS
ATCCTAAAGTGCATCAAGGT+TGG 0.542256 2:-7220817 MsG0280006864.01.T01:CDS
TATTAATCAAAGACCAAAGG+AGG 0.543306 2:+7218802 None:intergenic
TGCACTTGTGAATAAAACAT+TGG 0.548353 2:-7219142 MsG0280006864.01.T01:CDS
CTTGTGAATAAAACATTGGA+TGG 0.548661 2:-7219138 MsG0280006864.01.T01:CDS
TAAGACCAGTGCCCGAAACC+AGG 0.563404 2:-7220753 MsG0280006864.01.T01:intron
AGGTCTTAAGTGAGGTGAGA+AGG 0.577836 2:-7219608 MsG0280006864.01.T01:CDS
TCTACAACCCTCCATGACAC+TGG 0.578955 2:+7219093 None:intergenic
AGTTTGTGTCACCAACTCTA+GGG 0.582592 2:+7219418 None:intergenic
GTATTTGCTAGCCCTAGAGT+TGG 0.582655 2:-7219429 MsG0280006864.01.T01:CDS
AATACCTTGTGCCAGTGTCA+TGG 0.585230 2:-7219104 MsG0280006864.01.T01:CDS
CTATGTATTTGAAGAGTCCA+GGG 0.587725 2:-7219263 MsG0280006864.01.T01:CDS
GGAGGCTTATTTGCATGGAG+TGG 0.592309 2:-7219217 MsG0280006864.01.T01:CDS
GATATTGTTATTGCTTGGAG+AGG 0.596230 2:-7220929 MsG0280006864.01.T01:CDS
AATGATCCTAAAGTGCATCA+AGG 0.597945 2:-7220821 MsG0280006864.01.T01:CDS
CGTAGTATATATGAAGGTGA+AGG 0.606939 2:-7221437 MsG0280006864.01.T01:exon
ATTTATTAATCAAAGACCAA+AGG 0.608405 2:+7218799 None:intergenic
ATGGAAGCTTATGGATCATG+AGG 0.613240 2:-7219037 MsG0280006864.01.T01:CDS
CCCTCCATGACACTGGCACA+AGG 0.616143 2:+7219100 None:intergenic
CGTCGTAGATCAATGTCAAG+AGG 0.622571 2:+7221256 None:intergenic
ATTAATCAAAGACCAAAGGA+GGG 0.624748 2:+7218803 None:intergenic
GTCGTAGATCAATGTCAAGA+GGG 0.625735 2:+7221257 None:intergenic
AGCGGCGAAAATCATTGGAT+AGG 0.626832 2:-7221289 MsG0280006864.01.T01:CDS
GGTATGGTTCAACAATCAGA+TGG 0.636286 2:-7219063 MsG0280006864.01.T01:CDS
ACCTTGTGCCAGTGTCATGG+AGG 0.647161 2:-7219101 MsG0280006864.01.T01:CDS
ATTTGTGCAGGTCTTAAGTG+AGG 0.649606 2:-7219616 MsG0280006864.01.T01:intron
TAGCAATCATGATCAAAAGG+TGG 0.653386 2:-7221378 MsG0280006864.01.T01:CDS
TTGCTGTGGCTAATGATGAA+GGG 0.655838 2:-7220973 MsG0280006864.01.T01:CDS
ATGAAGGGAAAGATGTGTTG+GGG 0.669413 2:-7220958 MsG0280006864.01.T01:CDS
CCTTGTGCCAGTGTCATGGA+GGG 0.679650 2:-7219100 MsG0280006864.01.T01:CDS
AATTGCAACTCTAAATGCAG+TGG 0.688195 2:-7219520 MsG0280006864.01.T01:CDS
AACAAATGGCAACAACTCAG+CGG 0.706384 2:-7221307 MsG0280006864.01.T01:CDS
ATGGAGTGGCAGGAACACAA+GGG 0.725550 2:-7219203 MsG0280006864.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACCAAAAAAAATATTAAAT+TGG - Chr2:7220160-7220179 MsG0280006864.01.T01:intron 10.0%
!! ACACTTTAATTAAAAAAAAT+TGG - Chr2:7220576-7220595 MsG0280006864.01.T01:intron 10.0%
!! TAAACAAAAATTTATATTTG+TGG - Chr2:7220486-7220505 MsG0280006864.01.T01:intron 10.0%
!!! AACCAATTTAATATTTTTTT+TGG + Chr2:7220165-7220184 None:intergenic 10.0%
!!! ATATCAATTATTTTATTTGA+GGG + Chr2:7219931-7219950 None:intergenic 10.0%
!!! TAGTATTTTTTTCTTTAAAA+CGG - Chr2:7219541-7219560 MsG0280006864.01.T01:CDS 10.0%
!!! TATATCAATTATTTTATTTG+AGG + Chr2:7219932-7219951 None:intergenic 10.0%
!! AAAAAAAAAATCAAGGAAAA+AGG - Chr2:7219905-7219924 MsG0280006864.01.T01:intron 15.0%
!! AAAATAAAAATTCAAGTGTT+TGG - Chr2:7220418-7220437 MsG0280006864.01.T01:intron 15.0%
!! CATAATGAAAAACTAATATA+TGG + Chr2:7219750-7219769 None:intergenic 15.0%
!! TATAATTGAGAATTTATTCA+TGG - Chr2:7221259-7221278 MsG0280006864.01.T01:CDS 15.0%
!! TTTAACTACTAAAATCAATA+TGG - Chr2:7219798-7219817 MsG0280006864.01.T01:intron 15.0%
!! TTTGTTACAGAATATTTAAA+AGG + Chr2:7219154-7219173 None:intergenic 15.0%
!!! AGATTATATTAGTTTGATAT+TGG + Chr2:7220388-7220407 None:intergenic 15.0%
!!! AGTATTTTTTTCTTTAAAAC+GGG - Chr2:7219542-7219561 MsG0280006864.01.T01:CDS 15.0%
!!! TATCATTATTTTTTAGTTTG+AGG - Chr2:7221289-7221308 MsG0280006864.01.T01:CDS 15.0%
!!! TCTAAAGTTTTTTTAGAAAA+TGG - Chr2:7219124-7219143 MsG0280006864.01.T01:CDS 15.0%
!!! TGATTTTAGTAGTTAAAATA+TGG + Chr2:7219795-7219814 None:intergenic 15.0%
!!! TTTTCCTTGATTTTTTTTTT+TGG + Chr2:7219905-7219924 None:intergenic 15.0%
!! AGAGAATAATATTAGAAAAG+AGG - Chr2:7219963-7219982 MsG0280006864.01.T01:intron 20.0%
!! ATTTATTAATCAAAGACCAA+AGG + Chr2:7221452-7221471 None:intergenic 20.0%
!! TATGAACATTATTGTATGTT+TGG - Chr2:7221422-7221441 MsG0280006864.01.T01:CDS 20.0%
!!! AAACCATATTGTTTTTTTCA+AGG + Chr2:7219830-7219849 None:intergenic 20.0%
!!! AAATGATTGATTAGTTGTTA+AGG - Chr2:7220445-7220464 MsG0280006864.01.T01:intron 20.0%
!!! AACCATATTGTTTTTTTCAA+GGG + Chr2:7219829-7219848 None:intergenic 20.0%
!!! ATTTTTGTTTATTTGCTTGA+TGG + Chr2:7220477-7220496 None:intergenic 20.0%
!!! GTATTTTTTTCTTTAAAACG+GGG - Chr2:7219543-7219562 MsG0280006864.01.T01:CDS 20.0%
!!! TATATGACTATTTCTTTAGT+TGG + Chr2:7220228-7220247 None:intergenic 20.0%
!!! TATTTTAAGTTGGCATAATT+TGG - Chr2:7221010-7221029 MsG0280006864.01.T01:CDS 20.0%
!!! TATTTTTTTCTTTAAAACGG+GGG - Chr2:7219544-7219563 MsG0280006864.01.T01:CDS 20.0%
!!! TCTTATTTACAAGTTTATTG+TGG - Chr2:7220252-7220271 MsG0280006864.01.T01:intron 20.0%
! ACCTCCAAAAAAAAAAATCA+AGG - Chr2:7219898-7219917 MsG0280006864.01.T01:intron 25.0%
! ATGCTCAATAAAGCAAATAT+AGG - Chr2:7219644-7219663 MsG0280006864.01.T01:intron 25.0%
! CATATATCTCTTTCAAAGTT+TGG + Chr2:7219701-7219720 None:intergenic 25.0%
! GAAATAAGCATAACTATAAC+AGG - Chr2:7220687-7220706 MsG0280006864.01.T01:intron 25.0%
! TAATATAGTTTAGTCCATGT+AGG + Chr2:7220335-7220354 None:intergenic 25.0%
! TTCCCTTGAAAAAAACAATA+TGG - Chr2:7219824-7219843 MsG0280006864.01.T01:intron 25.0%
! TTTGTACGTAGTATATATGA+AGG - Chr2:7218805-7218824 MsG0280006864.01.T01:three_prime_UTR 25.0%
!! ATTTAGAACATAGATTTACC+TGG + Chr2:7219516-7219535 None:intergenic 25.0%
!! TAATGATCTTCAGTTTCTTT+TGG - Chr2:7219366-7219385 MsG0280006864.01.T01:CDS 25.0%
!!! ATATTTTTATCATGTGAGCT+AGG + Chr2:7220051-7220070 None:intergenic 25.0%
!!! CTGCTTGTTTTTTTTATGTA+TGG + Chr2:7218848-7218867 None:intergenic 25.0%
!!! TCCTTGATTTTTTTTTTTGG+AGG + Chr2:7219902-7219921 None:intergenic 25.0%
AACATAGATTTACCTGGTTT+CGG + Chr2:7219510-7219529 None:intergenic 30.0%
AACCAGATGGTTTATGAAAA+AGG + Chr2:7219677-7219696 None:intergenic 30.0%
AGAATTCAACTTAGGTAACA+TGG - Chr2:7220548-7220567 MsG0280006864.01.T01:intron 30.0%
AGTAAGGAATCAAATTGGAT+TGG - Chr2:7219247-7219266 MsG0280006864.01.T01:CDS 30.0%
ATTAATCAAAGACCAAAGGA+GGG + Chr2:7221448-7221467 None:intergenic 30.0%
CCAAAAATATGACATTGTTG+CGG + Chr2:7220000-7220019 None:intergenic 30.0%
CTGCTATTACCAAATACTTT+AGG + Chr2:7219400-7219419 None:intergenic 30.0%
CTTGTGAATAAAACATTGGA+TGG - Chr2:7221110-7221129 MsG0280006864.01.T01:CDS 30.0%
GACTTTATAATAAACGCTTC+CGG + Chr2:7219208-7219227 None:intergenic 30.0%
GTAAGGAATCAAATTGGATT+GGG - Chr2:7219248-7219267 MsG0280006864.01.T01:CDS 30.0%
TAGTGGTAAGAATTCAACTT+AGG - Chr2:7220540-7220559 MsG0280006864.01.T01:intron 30.0%
TATTAATCAAAGACCAAAGG+AGG + Chr2:7221449-7221468 None:intergenic 30.0%
TGCACTTGTGAATAAAACAT+TGG - Chr2:7221106-7221125 MsG0280006864.01.T01:CDS 30.0%
! AATATGGTTTGTGTATTACG+TGG - Chr2:7219840-7219859 MsG0280006864.01.T01:intron 30.0%
! ATATGGTTTGTGTATTACGT+GGG - Chr2:7219841-7219860 MsG0280006864.01.T01:intron 30.0%
! TGGATCTTCAGAAGTATAAA+TGG + Chr2:7219461-7219480 None:intergenic 30.0%
! TTAGCGAAAATCTTTTCCAA+CGG - Chr2:7220183-7220202 MsG0280006864.01.T01:intron 30.0%
! TTCCAAACTACCCTTTTATT+GGG - Chr2:7220919-7220938 MsG0280006864.01.T01:CDS 30.0%
!! AACGATACAAACTTTGGAAT+GGG - Chr2:7219342-7219361 MsG0280006864.01.T01:CDS 30.0%
!! TATTTCTTTAGTTGGTCTTG+TGG + Chr2:7220220-7220239 None:intergenic 30.0%
!! TGATAATTATCGATTTGTGC+AGG - Chr2:7220620-7220639 MsG0280006864.01.T01:intron 30.0%
!! TTTAAGTTGGCATAATTTGG+AGG - Chr2:7221013-7221032 MsG0280006864.01.T01:CDS 30.0%
!! TTTTTGGAGGTCTAAAACAA+GGG + Chr2:7219889-7219908 None:intergenic 30.0%
!!! TTTTTTGGAGGTCTAAAACA+AGG + Chr2:7219890-7219909 None:intergenic 30.0%
AATGATCCTAAAGTGCATCA+AGG - Chr2:7219427-7219446 MsG0280006864.01.T01:CDS 35.0%
AATTGCAACTCTAAATGCAG+TGG - Chr2:7220728-7220747 MsG0280006864.01.T01:intron 35.0%
AATTTGGAGGCTTATTTGCA+TGG - Chr2:7221026-7221045 MsG0280006864.01.T01:CDS 35.0%
ACATAGATTTACCTGGTTTC+GGG + Chr2:7219509-7219528 None:intergenic 35.0%
ACATCTGCATACCCAATAAA+AGG + Chr2:7220933-7220952 None:intergenic 35.0%
ACCACTAAATTGATGTCTCA+TGG + Chr2:7220527-7220546 None:intergenic 35.0%
ACTATAACAGGTCACAGTTT+AGG - Chr2:7220699-7220718 MsG0280006864.01.T01:intron 35.0%
AGCCTACAAATGTGTTAGAT+TGG - Chr2:7220309-7220328 MsG0280006864.01.T01:intron 35.0%
AGTCTTTATCTAGAGAAGCT+TGG - Chr2:7219224-7219243 MsG0280006864.01.T01:CDS 35.0%
ATCTCACACATACAAAATCG+AGG + Chr2:7220112-7220131 None:intergenic 35.0%
ATGTCTCATGGACATTCAAT+CGG + Chr2:7220515-7220534 None:intergenic 35.0%
CATAATCCACTATGGTGAAA+TGG - Chr2:7219018-7219037 MsG0280006864.01.T01:exon 35.0%
CATCTGCATACCCAATAAAA+GGG + Chr2:7220932-7220951 None:intergenic 35.0%
CGTAGTATATATGAAGGTGA+AGG - Chr2:7218811-7218830 MsG0280006864.01.T01:three_prime_UTR 35.0%
CTATGTATTTGAAGAGTCCA+GGG - Chr2:7220985-7221004 MsG0280006864.01.T01:CDS 35.0%
CTTGGAGTAAGGAATCAAAT+TGG - Chr2:7219242-7219261 MsG0280006864.01.T01:CDS 35.0%
GAGTAGCAATCATGATCAAA+AGG - Chr2:7218867-7218886 MsG0280006864.01.T01:three_prime_UTR 35.0%
GTTAGTTGAGACGTTATGAT+AGG - Chr2:7220078-7220097 MsG0280006864.01.T01:intron 35.0%
TACCCAATAAAAGGGTAGTT+TGG + Chr2:7220924-7220943 None:intergenic 35.0%
TAGCAATCATGATCAAAAGG+TGG - Chr2:7218870-7218889 MsG0280006864.01.T01:three_prime_UTR 35.0%
TCCATGAGACATCAATTTAG+TGG - Chr2:7220523-7220542 MsG0280006864.01.T01:intron 35.0%
TCTATGTATTTGAAGAGTCC+AGG - Chr2:7220984-7221003 MsG0280006864.01.T01:CDS 35.0%
TGCCAACTTAAAATATTCCC+TGG + Chr2:7221005-7221024 None:intergenic 35.0%
TGTGGAAATAATTTGACCGT+TGG + Chr2:7220202-7220221 None:intergenic 35.0%
TTCAACAATCAGATGGTTCA+TGG - Chr2:7221192-7221211 MsG0280006864.01.T01:CDS 35.0%
TTTATTGGGTATGCAGATGT+TGG - Chr2:7220933-7220952 MsG0280006864.01.T01:CDS 35.0%
! CCGCAACAATGTCATATTTT+TGG - Chr2:7219997-7220016 MsG0280006864.01.T01:intron 35.0%
! GAACGATACAAACTTTGGAA+TGG - Chr2:7219341-7219360 MsG0280006864.01.T01:CDS 35.0%
! GAAGGGATATTGTTATTGCT+TGG - Chr2:7219314-7219333 MsG0280006864.01.T01:CDS 35.0%
! GATATTGTTATTGCTTGGAG+AGG - Chr2:7219319-7219338 MsG0280006864.01.T01:CDS 35.0%
! GTTCCAAACTACCCTTTTAT+TGG - Chr2:7220918-7220937 MsG0280006864.01.T01:CDS 35.0%
! GTTTCTGCTCCTAAAGTATT+TGG - Chr2:7219388-7219407 MsG0280006864.01.T01:CDS 35.0%
! TTTTATGTATGGAACCAGAG+AGG + Chr2:7218837-7218856 None:intergenic 35.0%
!! GTCCAGGGAATATTTTAAGT+TGG - Chr2:7221000-7221019 MsG0280006864.01.T01:CDS 35.0%
!! GTCTTATTGAAGGGAGATTT+TGG + Chr2:7219481-7219500 None:intergenic 35.0%
!! TGCCTTTTTCATAAACCATC+TGG - Chr2:7219672-7219691 MsG0280006864.01.T01:intron 35.0%
AACAAATGGCAACAACTCAG+CGG - Chr2:7218941-7218960 MsG0280006864.01.T01:three_prime_UTR 40.0%
AAGTTTGTGTCACCAACTCT+AGG + Chr2:7220834-7220853 None:intergenic 40.0%
ACAGCAGCATAGCAAACAAA+TGG - Chr2:7218927-7218946 MsG0280006864.01.T01:three_prime_UTR 40.0%
AGATGGTTCATGGAAGCTTA+TGG - Chr2:7221202-7221221 MsG0280006864.01.T01:CDS 40.0%
AGTTTGTGTCACCAACTCTA+GGG + Chr2:7220833-7220852 None:intergenic 40.0%
ATCCTAAAGTGCATCAAGGT+TGG - Chr2:7219431-7219450 MsG0280006864.01.T01:CDS 40.0%
ATCTAGAGAAGCTTGGAGTA+AGG - Chr2:7219231-7219250 MsG0280006864.01.T01:CDS 40.0%
ATGAAGGGAAAGATGTGTTG+GGG - Chr2:7219290-7219309 MsG0280006864.01.T01:CDS 40.0%
ATGGAAGCTTATGGATCATG+AGG - Chr2:7221211-7221230 MsG0280006864.01.T01:CDS 40.0%
ATGGAGGGTTGTAGAGAATA+AGG - Chr2:7221163-7221182 MsG0280006864.01.T01:CDS 40.0%
ATTTGTGCAGGTCTTAAGTG+AGG - Chr2:7220632-7220651 MsG0280006864.01.T01:intron 40.0%
CAAAGTTTGCAGGTAGTTGT+AGG - Chr2:7219080-7219099 MsG0280006864.01.T01:CDS 40.0%
CGACGTTACATAATCCACTA+TGG - Chr2:7219010-7219029 MsG0280006864.01.T01:exon 40.0%
GACTTCCGAAATCAATGTTC+CGG - Chr2:7219186-7219205 MsG0280006864.01.T01:CDS 40.0%
GAGAGGAACGATACAAACTT+TGG - Chr2:7219336-7219355 MsG0280006864.01.T01:CDS 40.0%
GATGAAGGGAAAGATGTGTT+GGG - Chr2:7219289-7219308 MsG0280006864.01.T01:CDS 40.0%
GCAAATACTATGGCTGTAAC+TGG + Chr2:7220807-7220826 None:intergenic 40.0%
GCAGTGGACATTGTTACAAA+TGG - Chr2:7220744-7220763 MsG0280006864.01.T01:intron 40.0%
GGCCAATCTAACACATTTGT+AGG + Chr2:7220314-7220333 None:intergenic 40.0%
GGTATGGTTCAACAATCAGA+TGG - Chr2:7221185-7221204 MsG0280006864.01.T01:CDS 40.0%
GGTTGTAGAGAATAAGGGTA+TGG - Chr2:7221169-7221188 MsG0280006864.01.T01:CDS 40.0%
GTCGTAGATCAATGTCAAGA+GGG + Chr2:7218994-7219013 None:intergenic 40.0%
TACCAACCTTGATGCACTTT+AGG + Chr2:7219436-7219455 None:intergenic 40.0%
TGATGAAGGGAAAGATGTGT+TGG - Chr2:7219288-7219307 MsG0280006864.01.T01:CDS 40.0%
TGGAGGGTTGTAGAGAATAA+GGG - Chr2:7221164-7221183 MsG0280006864.01.T01:CDS 40.0%
TTCAAAGTTTGGCAACCAGA+TGG + Chr2:7219690-7219709 None:intergenic 40.0%
TTGCTGTGGCTAATGATGAA+GGG - Chr2:7219275-7219294 MsG0280006864.01.T01:CDS 40.0%
! TCTAGGGCTAGCAAATACTA+TGG + Chr2:7220817-7220836 None:intergenic 40.0%
!! ATGTGTTAGATTGGCCTACA+TGG - Chr2:7220318-7220337 MsG0280006864.01.T01:intron 40.0%
!! GAGAAAGCATCAAAGTTTGC+AGG - Chr2:7219070-7219089 MsG0280006864.01.T01:CDS 40.0%
AACTCAGCGGCGAAAATCAT+TGG - Chr2:7218954-7218973 MsG0280006864.01.T01:three_prime_UTR 45.0%
AATACCTTGTGCCAGTGTCA+TGG - Chr2:7221144-7221163 MsG0280006864.01.T01:CDS 45.0%
AGCGGCGAAAATCATTGGAT+AGG - Chr2:7218959-7218978 MsG0280006864.01.T01:three_prime_UTR 45.0%
AGGTCTTAAGTGAGGTGAGA+AGG - Chr2:7220640-7220659 MsG0280006864.01.T01:intron 45.0%
CGTCGTAGATCAATGTCAAG+AGG + Chr2:7218995-7219014 None:intergenic 45.0%
CTTAAGTGAGGTGAGAAGGT+TGG - Chr2:7220644-7220663 MsG0280006864.01.T01:intron 45.0%
GAAAGATGTGTTGGGGAGAA+GGG - Chr2:7219297-7219316 MsG0280006864.01.T01:CDS 45.0%
GTTGCTGTGGCTAATGATGA+AGG - Chr2:7219274-7219293 MsG0280006864.01.T01:CDS 45.0%
TGTGTATTACGTGGGTCCAA+GGG - Chr2:7219849-7219868 MsG0280006864.01.T01:intron 45.0%
TTGTATGTTTGGCCCTCCTT+TGG - Chr2:7221433-7221452 MsG0280006864.01.T01:exon 45.0%
TTGTGTATTACGTGGGTCCA+AGG - Chr2:7219848-7219867 MsG0280006864.01.T01:intron 45.0%
! GCTATGCTGCTGTTGTTGTT+TGG + Chr2:7218920-7218939 None:intergenic 45.0%
! GGAGGTCTAAAACAAGGGTT+TGG + Chr2:7219884-7219903 None:intergenic 45.0%
! GTATTTGCTAGCCCTAGAGT+TGG - Chr2:7220819-7220838 MsG0280006864.01.T01:CDS 45.0%
!! CGCTTCCGGAACATTGATTT+CGG + Chr2:7219194-7219213 None:intergenic 45.0%
!! CTTGTGCCATTTCACCATAG+TGG + Chr2:7219027-7219046 None:intergenic 45.0%
!! TTGGATTGGGTTTGTTGCTG+TGG - Chr2:7219261-7219280 MsG0280006864.01.T01:CDS 45.0%
AAGTGAGGTGAGAAGGTTGG+TGG - Chr2:7220647-7220666 MsG0280006864.01.T01:intron 50.0%
ATGACATTGTTGCGGCATGC+TGG + Chr2:7219992-7220011 None:intergenic 50.0%
ATGGAGTGGCAGGAACACAA+GGG - Chr2:7221045-7221064 MsG0280006864.01.T01:CDS 50.0%
ATTTACCTGGTTTCGGGCAC+TGG + Chr2:7219503-7219522 None:intergenic 50.0%
GCTTATTTGCATGGAGTGGC+AGG - Chr2:7221035-7221054 MsG0280006864.01.T01:CDS 50.0%
GGAAAGATGTGTTGGGGAGA+AGG - Chr2:7219296-7219315 MsG0280006864.01.T01:CDS 50.0%
GGAGGCTTATTTGCATGGAG+TGG - Chr2:7221031-7221050 MsG0280006864.01.T01:CDS 50.0%
TATGAAGGTGAAGGCCTCTC+TGG - Chr2:7218820-7218839 MsG0280006864.01.T01:three_prime_UTR 50.0%
TCTACAACCCTCCATGACAC+TGG + Chr2:7221158-7221177 None:intergenic 50.0%
!! CGGGCACTGGTCTTATTGAA+GGG + Chr2:7219490-7219509 None:intergenic 50.0%
!! TCGGGCACTGGTCTTATTGA+AGG + Chr2:7219491-7219510 None:intergenic 50.0%
ACATTGTTGCGGCATGCTGG+TGG + Chr2:7219989-7220008 None:intergenic 55.0%
ACCTTGTGCCAGTGTCATGG+AGG - Chr2:7221147-7221166 MsG0280006864.01.T01:CDS 55.0%
CATGGAGTGGCAGGAACACA+AGG - Chr2:7221044-7221063 MsG0280006864.01.T01:CDS 55.0%
CCTTGTGCCAGTGTCATGGA+GGG - Chr2:7221148-7221167 MsG0280006864.01.T01:CDS 55.0%
TAAGACCAGTGCCCGAAACC+AGG - Chr2:7219495-7219514 MsG0280006864.01.T01:CDS 55.0%
ACGTGGGTCCAAGGGTGAAG+CGG - Chr2:7219857-7219876 MsG0280006864.01.T01:intron 60.0%
CCCTCCATGACACTGGCACA+AGG + Chr2:7221151-7221170 None:intergenic 60.0%
! GGTTTGGTCCGCTTCACCCT+TGG + Chr2:7219868-7219887 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 7218784 7221486 7218784 ID=MsG0280006864.01;Name=MsG0280006864.01
Chr2 mRNA 7218784 7221486 7218784 ID=MsG0280006864.01.T01;Parent=MsG0280006864.01;Name=MsG0280006864.01.T01;_AED=0.22;_eAED=0.22;_QI=37|1|1|1|1|1|2|239|433
Chr2 exon 7218784 7219628 7218784 ID=MsG0280006864.01.T01:exon:2134;Parent=MsG0280006864.01.T01
Chr2 exon 7220754 7221486 7220754 ID=MsG0280006864.01.T01:exon:2133;Parent=MsG0280006864.01.T01
Chr2 five_prime_UTR 7221450 7221486 7221450 ID=MsG0280006864.01.T01:five_prime_utr;Parent=MsG0280006864.01.T01
Chr2 CDS 7220754 7221449 7220754 ID=MsG0280006864.01.T01:cds;Parent=MsG0280006864.01.T01
Chr2 CDS 7219023 7219628 7219023 ID=MsG0280006864.01.T01:cds;Parent=MsG0280006864.01.T01
Chr2 three_prime_UTR 7218784 7219022 7218784 ID=MsG0280006864.01.T01:three_prime_utr;Parent=MsG0280006864.01.T01
Gene Sequence

>MsG0280006864.01.T01

ATGAAGGTGAAGGCCTCTCTGGTTCCATACATAAAAAAAACAAGCAGAGTAGCAATCATGATCAAAAGGTGGAGAAACAATTTGAAGAAGAAGAAACCAAACAACAACAGCAGCATAGCAAACAAATGGCAACAACTCAGCGGCGAAAATCATTGGATAGGACTATTAGACCCTCTTGACATTGATCTACGACGTTACATAATCCACTATGGTGAAATGGCACAAGCAACATACGATGCTTTCAACACAGAGAAAGCATCAAAGTTTGCAGGTAGTTGTAGGTATGCAAAGAATGATTTCTTTTCTAAAGTTTTTTTAGAAAATGGAAATCCTTTTAAATATTCTGTAACAAAATTTATTTATGCGACTTCCGAAATCAATGTTCCGGAAGCGTTTATTATAAAGTCTTTATCTAGAGAAGCTTGGAGTAAGGAATCAAATTGGATTGGGTTTGTTGCTGTGGCTAATGATGAAGGGAAAGATGTGTTGGGGAGAAGGGATATTGTTATTGCTTGGAGAGGAACGATACAAACTTTGGAATGGGTTAATGATCTTCAGTTTCTTTTGGTTTCTGCTCCTAAAGTATTTGGTAATAGCAGTAATATTAATGATCCTAAAGTGCATCAAGGTTGGTACTCCATTTATACTTCTGAAGATCCAAAATCTCCCTTCAATAAGACCAGTGCCCGAAACCAGGTCTTAAGTGAGGTGAGAAGGTTGGTGGAGAAATACAAGAATGAAGAAATAAGCATAACTATAACAGGTCACAGTTTAGGTGCTGCAATTGCAACTCTAAATGCAGTGGACATTGTTACAAATGGTTATAACAAGTCAAGAGATTCATCTCTTAAAGCATCACCAGTTACAGCCATAGTATTTGCTAGCCCTAGAGTTGGTGACACAAACTTTCAAAAGCATTTTTCTAGCTACAAAGATCTTTCAACATTACGCATTCGAAATGAGTTAGATATTGTTCCAAACTACCCTTTTATTGGGTATGCAGATGTTGGTGAAGAGTTGAAAATTGACACAAGAAAATCTATGTATTTGAAGAGTCCAGGGAATATTTTAAGTTGGCATAATTTGGAGGCTTATTTGCATGGAGTGGCAGGAACACAAGGGAGTAAAAGAGTATTCAAACTTGAAGTTTGTCGTGATATTGCACTTGTGAATAAAACATTGGATGGTTTAAAAGATGAATACCTTGTGCCAGTGTCATGGAGGGTTGTAGAGAATAAGGGTATGGTTCAACAATCAGATGGTTCATGGAAGCTTATGGATCATGAGGATGACGAATTTTAA
Protein sequence

>MsG0280006864.01.T01

MKVKASLVPYIKKTSRVAIMIKRWRNNLKKKKPNNNSSIANKWQQLSGENHWIGLLDPLDIDLRRYIIHYGEMAQATYDAFNTEKASKFAGSCRYAKNDFFSKVFLENGNPFKYSVTKFIYATSEINVPEAFIIKSLSREAWSKESNWIGFVAVANDEGKDVLGRRDIVIAWRGTIQTLEWVNDLQFLLVSAPKVFGNSSNINDPKVHQGWYSIYTSEDPKSPFNKTSARNQVLSEVRRLVEKYKNEEISITITGHSLGAAIATLNAVDIVTNGYNKSRDSSLKASPVTAIVFASPRVGDTNFQKHFSSYKDLSTLRIRNELDIVPNYPFIGYADVGEELKIDTRKSMYLKSPGNILSWHNLEAYLHGVAGTQGSKRVFKLEVCRDIALVNKTLDGLKDEYLVPVSWRVVENKGMVQQSDGSWKLMDHEDDEF*