Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005188.01.T01 | XP_003591877.1 | 93.617 | 423 | 27 | 0 | 1 | 423 | 300 | 722 | 0 | 845 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005188.01.T01 | Q9SMK9 | 91.962 | 423 | 34 | 0 | 1 | 423 | 296 | 718 | 0 | 829 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005188.01.T01 | G7I515 | 93.617 | 423 | 27 | 0 | 1 | 423 | 300 | 722 | 0.0 | 845 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0180005188.01 | 0.832146 | 1.116480e-55 | 1.237079e-52 |
| MsG0080048700.01 | MsG0180005188.01 | 0.820211 | 7.732525e-53 | 6.079961e-50 |
| MsG0180000185.01 | MsG0180005188.01 | 0.868883 | 4.687538e-66 | 1.723538e-62 |
| MsG0180000360.01 | MsG0180005188.01 | 0.835186 | 1.943190e-56 | 2.358316e-53 |
| MsG0180001117.01 | MsG0180005188.01 | 0.806692 | 7.269764e-50 | 3.971468e-47 |
| MsG0180001237.01 | MsG0180005188.01 | 0.809373 | 1.953888e-50 | 1.145238e-47 |
| MsG0180001565.01 | MsG0180005188.01 | 0.823672 | 1.220840e-53 | 1.058282e-50 |
| MsG0180001566.01 | MsG0180005188.01 | 0.803770 | 2.975222e-49 | 1.507919e-46 |
| MsG0180003168.01 | MsG0180005188.01 | 0.827786 | 1.290848e-54 | 1.258786e-51 |
| MsG0180003242.01 | MsG0180005188.01 | 0.806092 | 9.731337e-50 | 5.235507e-47 |
| MsG0180004357.01 | MsG0180005188.01 | 0.820244 | 7.597886e-53 | 5.979547e-50 |
| MsG0180004989.01 | MsG0180005188.01 | 0.804920 | 1.713304e-49 | 8.945316e-47 |
| MsG0180005188.01 | MsG0180005189.01 | 0.894190 | 3.111417e-75 | 3.061199e-71 |
| MsG0180005188.01 | MsG0180005443.01 | 0.804148 | 2.483008e-49 | 1.270740e-46 |
| MsG0180005188.01 | MsG0180005451.01 | 0.808809 | 2.580745e-50 | 1.489930e-47 |
| MsG0180005188.01 | MsG0180006260.01 | 0.846689 | 1.855386e-59 | 3.219731e-56 |
| MsG0180005188.01 | MsG0280006692.01 | 0.821001 | 5.091111e-53 | 4.092370e-50 |
| MsG0180005188.01 | MsG0280006864.01 | 0.802007 | 6.880923e-49 | 3.333773e-46 |
| MsG0180005188.01 | MsG0280007706.01 | 0.800238 | 1.582749e-48 | 7.332328e-46 |
| MsG0180005188.01 | MsG0280007790.01 | 0.805165 | 1.523094e-49 | 8.001580e-47 |
| MsG0180005188.01 | MsG0280008270.01 | 0.809670 | 1.686844e-50 | 9.965723e-48 |
| MsG0180005188.01 | MsG0280008614.01 | 0.831528 | 1.586595e-55 | 1.726393e-52 |
| MsG0180005188.01 | MsG0280009118.01 | 0.800937 | 1.140247e-48 | 5.377108e-46 |
| MsG0180005188.01 | MsG0280009156.01 | 0.818345 | 2.057674e-52 | 1.536405e-49 |
| MsG0180005188.01 | MsG0280009509.01 | 0.861917 | 7.324803e-64 | 2.106021e-60 |
| MsG0180005188.01 | MsG0280009514.01 | 0.828960 | 6.724538e-55 | 6.785821e-52 |
| MsG0180005188.01 | MsG0280010001.01 | 0.839060 | 1.986365e-57 | 2.711411e-54 |
| MsG0180005188.01 | MsG0280010735.01 | 0.819607 | 1.062860e-52 | 8.217370e-50 |
| MsG0180005188.01 | MsG0280011293.01 | 0.813105 | 3.029216e-51 | 1.960199e-48 |
| MsG0180005188.01 | MsG0380013548.01 | 0.836223 | 1.061400e-56 | 1.329197e-53 |
| MsG0180005188.01 | MsG0380014576.01 | 0.800078 | 1.706367e-48 | 7.872940e-46 |
| MsG0180005188.01 | MsG0380015548.01 | 0.812241 | 4.679869e-51 | 2.959929e-48 |
| MsG0180005188.01 | MsG0380016236.01 | 0.819924 | 8.997183e-53 | 7.018381e-50 |
| MsG0180005188.01 | MsG0380016510.01 | 0.825172 | 5.419196e-54 | 4.902529e-51 |
| MsG0180005188.01 | MsG0380017240.01 | 0.840104 | 1.063062e-57 | 1.498812e-54 |
| MsG0180005188.01 | MsG0380017643.01 | 0.806624 | 7.514163e-50 | 4.097718e-47 |
| MsG0180005188.01 | MsG0480018230.01 | 0.831435 | 1.671935e-55 | 1.814128e-52 |
| MsG0180005188.01 | MsG0480018238.01 | 0.804749 | 1.860802e-49 | 9.672241e-47 |
| MsG0180005188.01 | MsG0480018256.01 | 0.836759 | 7.752695e-57 | 9.865297e-54 |
| MsG0180005188.01 | MsG0480018845.01 | 0.809409 | 1.919544e-50 | 1.126203e-47 |
| MsG0180005188.01 | MsG0480018846.01 | 0.818261 | 2.149624e-52 | 1.601153e-49 |
| MsG0180005188.01 | MsG0480019034.01 | 0.803257 | 3.800115e-49 | 1.900765e-46 |
| MsG0180005188.01 | MsG0480019170.01 | 0.832371 | 9.818793e-56 | 1.095289e-52 |
| MsG0180005188.01 | MsG0480020242.01 | 0.813321 | 2.716148e-51 | 1.768126e-48 |
| MsG0180005188.01 | MsG0480021156.01 | 0.818767 | 1.650899e-52 | 1.247048e-49 |
| MsG0180005188.01 | MsG0480021158.01 | 0.831250 | 1.857254e-55 | 2.004004e-52 |
| MsG0180005188.01 | MsG0480021225.01 | 0.811895 | 5.569584e-51 | 3.489392e-48 |
| MsG0180005188.01 | MsG0480021705.01 | 0.838372 | 2.990080e-57 | 3.996852e-54 |
| MsG0180005188.01 | MsG0480021923.01 | 0.801587 | 8.391839e-49 | 4.022588e-46 |
| MsG0180005188.01 | MsG0480022228.01 | 0.825113 | 5.596595e-54 | 5.054332e-51 |
| MsG0180005188.01 | MsG0480022331.01 | 0.805010 | 1.641113e-49 | 8.587256e-47 |
| MsG0180005188.01 | MsG0480022598.01 | 0.801523 | 8.648153e-49 | 4.138972e-46 |
| MsG0180005188.01 | MsG0480022719.01 | 0.811899 | 5.557999e-51 | 3.482544e-48 |
| MsG0180005188.01 | MsG0480022738.01 | 0.854176 | 1.465466e-61 | 3.245319e-58 |
| MsG0180005188.01 | MsG0480023053.01 | 0.857961 | 1.142120e-62 | 2.868793e-59 |
| MsG0180005188.01 | MsG0480023196.01 | 0.812803 | 3.527046e-51 | 2.264026e-48 |
| MsG0180005188.01 | MsG0480023421.01 | 0.846220 | 2.490957e-59 | 4.256880e-56 |
| MsG0180005188.01 | MsG0480023620.01 | 0.871202 | 8.179392e-67 | 3.271369e-63 |
| MsG0180005188.01 | MsG0480023718.01 | 0.800059 | 1.721523e-48 | 7.939043e-46 |
| MsG0180005188.01 | MsG0580024157.01 | 0.829007 | 6.549709e-55 | 6.618456e-52 |
| MsG0180005188.01 | MsG0580024793.01 | 0.837923 | 3.902351e-57 | 5.145377e-54 |
| MsG0180005188.01 | MsG0580025072.01 | 0.838465 | 2.829955e-57 | 3.793635e-54 |
| MsG0180005188.01 | MsG0580025352.01 | 0.829486 | 5.011186e-55 | 5.135942e-52 |
| MsG0180005188.01 | MsG0580027208.01 | 0.806880 | 6.635623e-50 | 3.642717e-47 |
| MsG0180005188.01 | MsG0680030436.01 | 0.820272 | 7.488224e-53 | 5.897803e-50 |
| MsG0180005188.01 | MsG0680030621.01 | 0.806360 | 8.543158e-50 | 4.627644e-47 |
| MsG0180005188.01 | MsG0680030679.01 | 0.814805 | 1.277540e-51 | 8.657647e-49 |
| MsG0180005188.01 | MsG0680030740.01 | 0.836783 | 7.644987e-57 | 9.735090e-54 |
| MsG0180005188.01 | MsG0680031877.01 | 0.803432 | 3.495414e-49 | 1.756257e-46 |
| MsG0180005188.01 | MsG0680031975.01 | 0.803706 | 3.066860e-49 | 1.551782e-46 |
| MsG0180005188.01 | MsG0680033793.01 | 0.816179 | 6.318552e-52 | 4.445669e-49 |
| MsG0180005188.01 | MsG0780038112.01 | 0.803552 | 3.301045e-49 | 1.663797e-46 |
| MsG0180005188.01 | MsG0780039654.01 | 0.842688 | 2.220057e-58 | 3.392279e-55 |
| MsG0180005188.01 | MsG0780039655.01 | 0.830247 | 3.269314e-55 | 3.425721e-52 |
| MsG0180005188.01 | MsG0780039712.01 | 0.861638 | 8.916540e-64 | 2.539627e-60 |
| MsG0180005188.01 | MsG0780040290.01 | 0.839409 | 1.611977e-57 | 2.224310e-54 |
| MsG0180005188.01 | MsG0780040488.01 | 0.815145 | 1.073948e-51 | 7.344675e-49 |
| MsG0180005188.01 | MsG0780040541.01 | 0.844644 | 6.653833e-59 | 1.081179e-55 |
| MsG0180005188.01 | MsG0780040564.01 | 0.839072 | 1.971263e-57 | 2.691818e-54 |
| MsG0180005188.01 | MsG0780040586.01 | 0.824895 | 6.300429e-54 | 5.655001e-51 |
| MsG0180005188.01 | MsG0780040880.01 | 0.801460 | 8.911648e-49 | 4.258212e-46 |
| MsG0180005188.01 | MsG0780041787.01 | 0.810881 | 9.244362e-51 | 5.639027e-48 |
| MsG0180005188.01 | MsG0880042836.01 | 0.802631 | 5.120128e-49 | 2.520405e-46 |
| MsG0180005188.01 | MsG0880042845.01 | 0.852138 | 5.619107e-61 | 1.162985e-57 |
| MsG0180005188.01 | MsG0880043921.01 | 0.805464 | 1.318039e-49 | 6.978055e-47 |
| MsG0180005188.01 | MsG0880044075.01 | 0.803277 | 3.763784e-49 | 1.883570e-46 |
| MsG0180005188.01 | MsG0880044803.01 | 0.861510 | 9.750980e-64 | 2.766041e-60 |
| MsG0180005188.01 | MsG0880046027.01 | 0.822939 | 1.811456e-53 | 1.537938e-50 |
| MsG0180005188.01 | MsG0880046165.01 | 0.801234 | 9.915070e-49 | 4.710843e-46 |
| MsG0180005188.01 | MsG0880046186.01 | 0.820800 | 5.662984e-53 | 4.526101e-50 |
| MsG0180005188.01 | MsG0880046762.01 | 0.835143 | 1.992139e-56 | 2.414476e-53 |
| MsG0180005188.01 | MsG0880047226.01 | 0.812215 | 4.743340e-51 | 2.997929e-48 |
| MsG0180005188.01 | MsG0880047293.01 | 0.841217 | 5.433846e-58 | 7.928874e-55 |
| MsG0180005188.01 | MsG0880047337.01 | 0.823172 | 1.598185e-53 | 1.365792e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005188.01.T01 | MTR_1g094780 | 93.617 | 423 | 27 | 0 | 1 | 423 | 300 | 722 | 0.0 | 845 |
| MsG0180005188.01.T01 | MTR_1g064090 | 82.270 | 423 | 75 | 0 | 1 | 423 | 290 | 712 | 0.0 | 750 |
| MsG0180005188.01.T01 | MTR_7g101395 | 82.033 | 423 | 76 | 0 | 1 | 423 | 302 | 724 | 0.0 | 747 |
| MsG0180005188.01.T01 | MTR_7g101425 | 81.324 | 423 | 79 | 0 | 1 | 423 | 302 | 724 | 0.0 | 740 |
| MsG0180005188.01.T01 | MTR_2g094960 | 72.706 | 425 | 111 | 2 | 1 | 422 | 275 | 697 | 0.0 | 638 |
| MsG0180005188.01.T01 | MTR_5g098720 | 69.905 | 422 | 126 | 1 | 1 | 422 | 288 | 708 | 0.0 | 627 |
| MsG0180005188.01.T01 | MTR_2g095010 | 45.517 | 145 | 52 | 2 | 250 | 394 | 1 | 118 | 2.99e-35 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005188.01.T01 | AT3G53260 | 81.797 | 423 | 77 | 0 | 1 | 423 | 295 | 717 | 0.0 | 756 |
| MsG0180005188.01.T01 | AT2G37040 | 81.324 | 423 | 79 | 0 | 1 | 423 | 303 | 725 | 0.0 | 752 |
| MsG0180005188.01.T01 | AT3G10340 | 79.669 | 423 | 86 | 0 | 1 | 423 | 285 | 707 | 0.0 | 731 |
| MsG0180005188.01.T01 | AT5G04230 | 72.104 | 423 | 108 | 3 | 1 | 423 | 282 | 694 | 0.0 | 634 |
| MsG0180005188.01.T01 | AT5G04230 | 71.429 | 427 | 108 | 4 | 1 | 423 | 282 | 698 | 0.0 | 628 |
Find 90 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGCCAAGCTATTGATTTA+AGG | 0.183642 | 1:-88226541 | MsG0180005188.01.T01:CDS |
| CCTCAAAAGCTCCAATCTTT+TGG | 0.213487 | 1:+88226249 | None:intergenic |
| TATTGATGTTTCAAGGAATA+TGG | 0.225643 | 1:-88226939 | MsG0180005188.01.T01:CDS |
| AATTGAGGCTGCTGCTATTA+TGG | 0.231999 | 1:-88227155 | MsG0180005188.01.T01:CDS |
| CTACATTCCTTGATCATATT+AGG | 0.256307 | 1:+88226152 | None:intergenic |
| GCTAGGGTTCAAGTTGAAAA+TGG | 0.308466 | 1:-88226188 | MsG0180005188.01.T01:CDS |
| TGCTGACAGGTGAGAAAATT+AGG | 0.327586 | 1:-88226082 | MsG0180005188.01.T01:CDS |
| TGCTAGCCGCAATCCAAGTT+TGG | 0.335095 | 1:-88226765 | MsG0180005188.01.T01:CDS |
| AACCAAGATGTTAACTCTTT+AGG | 0.341255 | 1:-88226638 | MsG0180005188.01.T01:CDS |
| CGCAATCCAAGTTTGGATTA+CGG | 0.346277 | 1:-88226758 | MsG0180005188.01.T01:CDS |
| TGTCTCAGCTTCTGCATTAG+AGG | 0.360021 | 1:+88226335 | None:intergenic |
| AGTTCATAAGAGAAACTTTA+GGG | 0.361370 | 1:-88226112 | MsG0180005188.01.T01:CDS |
| AGATGGAAGTTATTATCTTA+AGG | 0.370273 | 1:-88227122 | MsG0180005188.01.T01:CDS |
| TCCTTCTCACAAAACCTTGA+TGG | 0.371743 | 1:+88226425 | None:intergenic |
| ATCCACTTATTGATGTTTCA+AGG | 0.393519 | 1:-88226946 | MsG0180005188.01.T01:CDS |
| GTAGCACAATCCTAGCAAAT+AGG | 0.399936 | 1:+88225957 | None:intergenic |
| TCAAGGAATATGGCACTTCA+TGG | 0.407244 | 1:-88226929 | MsG0180005188.01.T01:CDS |
| GTTGATCATGCATTGCAAAA+TGG | 0.407281 | 1:-88226305 | MsG0180005188.01.T01:CDS |
| ATCGCGTATCTATCTTGTTT+AGG | 0.408011 | 1:+88227061 | None:intergenic |
| TTTCCACCTCTTTAGGCAAA+AGG | 0.408359 | 1:+88226213 | None:intergenic |
| TTCCTTGAAACATCAATAAG+TGG | 0.432083 | 1:+88226944 | None:intergenic |
| AAGTTCATAAGAGAAACTTT+AGG | 0.433340 | 1:-88226113 | MsG0180005188.01.T01:CDS |
| CGGCTAGCAGTGAGATTTGA+AGG | 0.436480 | 1:+88226779 | None:intergenic |
| GTATTGAAGTTCTGAACAAT+AGG | 0.446436 | 1:+88226706 | None:intergenic |
| CGTTTAGCCATTGCTTCCAT+TGG | 0.450648 | 1:-88226860 | MsG0180005188.01.T01:CDS |
| GTTTCAACTTATGAATCAAA+TGG | 0.458671 | 1:+88227191 | None:intergenic |
| TGCATTGCAAAATGGTGATA+AGG | 0.460091 | 1:-88226297 | MsG0180005188.01.T01:CDS |
| ATTCTCACAGTTGGTGTCAA+TGG | 0.460157 | 1:-88226458 | MsG0180005188.01.T01:CDS |
| CGAACATCACCGCAGTGGCT+TGG | 0.467832 | 1:-88227037 | MsG0180005188.01.T01:CDS |
| TTGAAGCCGTAATCCAAACT+TGG | 0.469165 | 1:+88226752 | None:intergenic |
| GATTTACTCAATGTTGTTGA+AGG | 0.472171 | 1:-88226404 | MsG0180005188.01.T01:CDS |
| TTCCACCTCTTTAGGCAAAA+GGG | 0.473173 | 1:+88226214 | None:intergenic |
| TTAGGGACAACTTTGCTGAC+AGG | 0.498076 | 1:-88226095 | MsG0180005188.01.T01:CDS |
| AGCCTAAAGAGTTAACATCT+TGG | 0.498300 | 1:+88226636 | None:intergenic |
| CTAAAGAGGTGGAAAATGCT+AGG | 0.500407 | 1:-88226205 | MsG0180005188.01.T01:CDS |
| TCCCATGTTGGATTGTCTAA+AGG | 0.502948 | 1:-88226000 | MsG0180005188.01.T01:CDS |
| ATCAAATGGTCAGTGAATTG+AGG | 0.509685 | 1:+88227205 | None:intergenic |
| TTCAGTTCCTAATATGATCA+AGG | 0.512088 | 1:-88226159 | MsG0180005188.01.T01:CDS |
| ACCAACTGTGAGAATTCTCT+TGG | 0.519185 | 1:+88226466 | None:intergenic |
| AGCAAACATAAGTTTACCAA+TGG | 0.521443 | 1:+88226844 | None:intergenic |
| AGTTTGGATTACGGCTTCAA+AGG | 0.525060 | 1:-88226749 | MsG0180005188.01.T01:CDS |
| ACCTGGTGAGGAATGTGATA+AGG | 0.527766 | 1:-88226057 | MsG0180005188.01.T01:CDS |
| TCTTGTTTAGGCTTCTGAAG+TGG | 0.531050 | 1:+88227073 | None:intergenic |
| TCTCTTATGAACTTGTACAA+TGG | 0.534620 | 1:+88226122 | None:intergenic |
| TTGATCATATTAGGAACTGA+AGG | 0.538404 | 1:+88226161 | None:intergenic |
| CTTTGAACATGAGTGGTGAC+AGG | 0.539854 | 1:+88226674 | None:intergenic |
| TGCTCCTCTACCTATTTGCT+AGG | 0.540972 | 1:-88225967 | None:intergenic |
| ACTTCATCCTGGTCAAATTG+AGG | 0.542522 | 1:-88227170 | MsG0180005188.01.T01:CDS |
| TATTGATTTAAGGCACATTG+AGG | 0.544153 | 1:-88226531 | MsG0180005188.01.T01:CDS |
| CAATCCTAGCAAATAGGTAG+AGG | 0.545332 | 1:+88225963 | None:intergenic |
| ATGGTGAGTTGCATCCATCA+AGG | 0.558706 | 1:-88226439 | MsG0180005188.01.T01:CDS |
| GAAAATTAGGTCACCTGGTG+AGG | 0.558787 | 1:-88226069 | MsG0180005188.01.T01:CDS |
| GCCAAGAGAATTCTCACAGT+TGG | 0.559513 | 1:-88226467 | MsG0180005188.01.T01:CDS |
| AATAAGTGGATTGTCATTCA+CGG | 0.561129 | 1:+88226958 | None:intergenic |
| AGAGGGTATGTAGCACTGCA+AGG | 0.563580 | 1:+88226353 | None:intergenic |
| TCACAGCACTGTGTGATGGA+AGG | 0.566358 | 1:-88226031 | MsG0180005188.01.T01:CDS |
| AATTTCCAAGGTACACCGAT+TGG | 0.566709 | 1:-88226902 | MsG0180005188.01.T01:CDS |
| AAGGTTTATTGATCCCATGT+TGG | 0.570229 | 1:-88226012 | MsG0180005188.01.T01:CDS |
| ATACACCAATCGGTGTACCT+TGG | 0.570750 | 1:+88226897 | None:intergenic |
| TGTGCCTTAAATCAATAGCT+TGG | 0.571902 | 1:+88226537 | None:intergenic |
| TTGGCACAATGCTACTAAGA+AGG | 0.573844 | 1:+88226556 | None:intergenic |
| ACGCAACACAGATGATTGAG+AGG | 0.575882 | 1:-88226991 | MsG0180005188.01.T01:CDS |
| TTATCCATCGATACACCAAT+CGG | 0.577310 | 1:+88226887 | None:intergenic |
| TAAAGAGGTGGAAAATGCTA+GGG | 0.582840 | 1:-88226204 | MsG0180005188.01.T01:CDS |
| CTCCTTTAGACAATCCAACA+TGG | 0.584218 | 1:+88225998 | None:intergenic |
| CATAAGTTGAAACTTCATCC+TGG | 0.584696 | 1:-88227181 | MsG0180005188.01.T01:CDS |
| AAGTTTACCAATGGAAGCAA+TGG | 0.586687 | 1:+88226853 | None:intergenic |
| TGATCATATTAGGAACTGAA+GGG | 0.589476 | 1:+88226162 | None:intergenic |
| GGTGAGAAAATTAGGTCACC+TGG | 0.591426 | 1:-88226074 | MsG0180005188.01.T01:CDS |
| GTCTCAGCTTCTGCATTAGA+GGG | 0.593808 | 1:+88226336 | None:intergenic |
| ACCTTATCACATTCCTCACC+AGG | 0.598892 | 1:+88226056 | None:intergenic |
| TGTTGGATTGTCTAAAGGAG+TGG | 0.600217 | 1:-88225995 | MsG0180005188.01.T01:CDS |
| CTTCATGGAGGAAATTTCCA+AGG | 0.609850 | 1:-88226914 | MsG0180005188.01.T01:CDS |
| TACACCGATTGGTGTATCGA+TGG | 0.610433 | 1:-88226891 | MsG0180005188.01.T01:CDS |
| GAGGGTATGTAGCACTGCAA+GGG | 0.617118 | 1:+88226354 | None:intergenic |
| GTGAATGACATATATAGCAA+CGG | 0.623158 | 1:-88226806 | MsG0180005188.01.T01:CDS |
| GCACAATGCTACTAAGAAGG+TGG | 0.624197 | 1:+88226559 | None:intergenic |
| GCAGCAGCCTCAATTTGACC+AGG | 0.628904 | 1:+88227163 | None:intergenic |
| CGTAATCCAAACTTGGATTG+CGG | 0.630042 | 1:+88226759 | None:intergenic |
| CGCAACACAGATGATTGAGA+GGG | 0.630794 | 1:-88226990 | MsG0180005188.01.T01:CDS |
| CAAAGGAGCAGAAATTGCAA+TGG | 0.637084 | 1:-88226732 | MsG0180005188.01.T01:CDS |
| TCCTTTAGACAATCCAACAT+GGG | 0.644402 | 1:+88225999 | None:intergenic |
| TGTCGAATCACTTCAATCTG+CGG | 0.647914 | 1:+88227013 | None:intergenic |
| ATTGCAAAATGGTGATAAGG+AGG | 0.652869 | 1:-88226294 | MsG0180005188.01.T01:CDS |
| GAGTGGTGACAGGATTAGCA+AGG | 0.667400 | 1:+88226684 | None:intergenic |
| TTCAGCACTTTGAACATGAG+TGG | 0.675639 | 1:+88226667 | None:intergenic |
| AGGAATATGGCACTTCATGG+AGG | 0.692048 | 1:-88226926 | MsG0180005188.01.T01:CDS |
| GATTGTCTAAAGGAGTGGAA+TGG | 0.692592 | 1:-88225990 | MsG0180005188.01.T01:CDS |
| CGATTCGAACATCACCGCAG+TGG | 0.703444 | 1:-88227042 | MsG0180005188.01.T01:CDS |
| ATCTGCGGTCCAAGCCACTG+CGG | 0.717350 | 1:+88227028 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATGGAACATATTTTAGA+TGG | - | Chr1:88226046-88226065 | MsG0180005188.01.T01:CDS | 20.0% |
| ! | AAGTTCATAAGAGAAACTTT+AGG | - | Chr1:88227072-88227091 | MsG0180005188.01.T01:CDS | 25.0% |
| ! | AGATGGAAGTTATTATCTTA+AGG | - | Chr1:88226063-88226082 | MsG0180005188.01.T01:CDS | 25.0% |
| ! | AGTTCATAAGAGAAACTTTA+GGG | - | Chr1:88227073-88227092 | MsG0180005188.01.T01:CDS | 25.0% |
| ! | GTTTCAACTTATGAATCAAA+TGG | + | Chr1:88225997-88226016 | None:intergenic | 25.0% |
| ! | TATTGATGTTTCAAGGAATA+TGG | - | Chr1:88226246-88226265 | MsG0180005188.01.T01:CDS | 25.0% |
| AACCAAGATGTTAACTCTTT+AGG | - | Chr1:88226547-88226566 | MsG0180005188.01.T01:CDS | 30.0% | |
| AGCAAACATAAGTTTACCAA+TGG | + | Chr1:88226344-88226363 | None:intergenic | 30.0% | |
| ATCCACTTATTGATGTTTCA+AGG | - | Chr1:88226239-88226258 | MsG0180005188.01.T01:CDS | 30.0% | |
| CTACATTCCTTGATCATATT+AGG | + | Chr1:88227036-88227055 | None:intergenic | 30.0% | |
| GATTTACTCAATGTTGTTGA+AGG | - | Chr1:88226781-88226800 | MsG0180005188.01.T01:CDS | 30.0% | |
| GTGAATGACATATATAGCAA+CGG | - | Chr1:88226379-88226398 | MsG0180005188.01.T01:CDS | 30.0% | |
| TCTCTTATGAACTTGTACAA+TGG | + | Chr1:88227066-88227085 | None:intergenic | 30.0% | |
| TGATCATATTAGGAACTGAA+GGG | + | Chr1:88227026-88227045 | None:intergenic | 30.0% | |
| TTCAGTTCCTAATATGATCA+AGG | - | Chr1:88227026-88227045 | MsG0180005188.01.T01:CDS | 30.0% | |
| TTCCTTGAAACATCAATAAG+TGG | + | Chr1:88226244-88226263 | None:intergenic | 30.0% | |
| TTGATCATATTAGGAACTGA+AGG | + | Chr1:88227027-88227046 | None:intergenic | 30.0% | |
| ! | ACCTCAATTTTCCAAAAGAT+TGG | - | Chr1:88226925-88226944 | MsG0180005188.01.T01:CDS | 30.0% |
| !! | AATAAGTGGATTGTCATTCA+CGG | + | Chr1:88226230-88226249 | None:intergenic | 30.0% |
| !! | GTATTGAAGTTCTGAACAAT+AGG | + | Chr1:88226482-88226501 | None:intergenic | 30.0% |
| !! | TATTGATTTAAGGCACATTG+AGG | - | Chr1:88226654-88226673 | MsG0180005188.01.T01:CDS | 30.0% |
| !! | TCCAATCTTTTGGAAAATTG+AGG | + | Chr1:88226929-88226948 | None:intergenic | 30.0% |
| AAGGTTTATTGATCCCATGT+TGG | - | Chr1:88227173-88227192 | MsG0180005188.01.T01:CDS | 35.0% | |
| AAGTTTACCAATGGAAGCAA+TGG | + | Chr1:88226335-88226354 | None:intergenic | 35.0% | |
| AGCCTAAAGAGTTAACATCT+TGG | + | Chr1:88226552-88226571 | None:intergenic | 35.0% | |
| ATCAAATGGTCAGTGAATTG+AGG | + | Chr1:88225983-88226002 | None:intergenic | 35.0% | |
| ATTGCAAAATGGTGATAAGG+AGG | - | Chr1:88226891-88226910 | MsG0180005188.01.T01:CDS | 35.0% | |
| GTTGATCATGCATTGCAAAA+TGG | - | Chr1:88226880-88226899 | MsG0180005188.01.T01:CDS | 35.0% | |
| TAAAGAGGTGGAAAATGCTA+GGG | - | Chr1:88226981-88227000 | MsG0180005188.01.T01:CDS | 35.0% | |
| TCCTTTAGACAATCCAACAT+GGG | + | Chr1:88227189-88227208 | None:intergenic | 35.0% | |
| TGCATTGCAAAATGGTGATA+AGG | - | Chr1:88226888-88226907 | MsG0180005188.01.T01:CDS | 35.0% | |
| TGTGCCTTAAATCAATAGCT+TGG | + | Chr1:88226651-88226670 | None:intergenic | 35.0% | |
| TTATCCATCGATACACCAAT+CGG | + | Chr1:88226301-88226320 | None:intergenic | 35.0% | |
| ! | ATCGCGTATCTATCTTGTTT+AGG | + | Chr1:88226127-88226146 | None:intergenic | 35.0% |
| ! | CATAAGTTGAAACTTCATCC+TGG | - | Chr1:88226004-88226023 | MsG0180005188.01.T01:CDS | 35.0% |
| !! | TGTGCCAAGCTATTGATTTA+AGG | - | Chr1:88226644-88226663 | MsG0180005188.01.T01:CDS | 35.0% |
| AATTGAGGCTGCTGCTATTA+TGG | - | Chr1:88226030-88226049 | MsG0180005188.01.T01:CDS | 40.0% | |
| AATTTCCAAGGTACACCGAT+TGG | - | Chr1:88226283-88226302 | MsG0180005188.01.T01:CDS | 40.0% | |
| ACCAACTGTGAGAATTCTCT+TGG | + | Chr1:88226722-88226741 | None:intergenic | 40.0% | |
| ACTTCATCCTGGTCAAATTG+AGG | - | Chr1:88226015-88226034 | MsG0180005188.01.T01:CDS | 40.0% | |
| AGTTTGGATTACGGCTTCAA+AGG | - | Chr1:88226436-88226455 | MsG0180005188.01.T01:CDS | 40.0% | |
| CAAAGGAGCAGAAATTGCAA+TGG | - | Chr1:88226453-88226472 | MsG0180005188.01.T01:CDS | 40.0% | |
| CGCAATCCAAGTTTGGATTA+CGG | - | Chr1:88226427-88226446 | MsG0180005188.01.T01:CDS | 40.0% | |
| CGTAATCCAAACTTGGATTG+CGG | + | Chr1:88226429-88226448 | None:intergenic | 40.0% | |
| CTAAAGAGGTGGAAAATGCT+AGG | - | Chr1:88226980-88226999 | MsG0180005188.01.T01:CDS | 40.0% | |
| CTCCTTTAGACAATCCAACA+TGG | + | Chr1:88227190-88227209 | None:intergenic | 40.0% | |
| CTTCATGGAGGAAATTTCCA+AGG | - | Chr1:88226271-88226290 | MsG0180005188.01.T01:CDS | 40.0% | |
| GATTGTCTAAAGGAGTGGAA+TGG | - | Chr1:88227195-88227214 | MsG0180005188.01.T01:CDS | 40.0% | |
| TCAAGGAATATGGCACTTCA+TGG | - | Chr1:88226256-88226275 | MsG0180005188.01.T01:CDS | 40.0% | |
| TCCCATGTTGGATTGTCTAA+AGG | - | Chr1:88227185-88227204 | MsG0180005188.01.T01:CDS | 40.0% | |
| TCCTTCTCACAAAACCTTGA+TGG | + | Chr1:88226763-88226782 | None:intergenic | 40.0% | |
| TGCTGACAGGTGAGAAAATT+AGG | - | Chr1:88227103-88227122 | MsG0180005188.01.T01:CDS | 40.0% | |
| TGTCGAATCACTTCAATCTG+CGG | + | Chr1:88226175-88226194 | None:intergenic | 40.0% | |
| TGTTGGATTGTCTAAAGGAG+TGG | - | Chr1:88227190-88227209 | MsG0180005188.01.T01:CDS | 40.0% | |
| TTCAGCACTTTGAACATGAG+TGG | + | Chr1:88226521-88226540 | None:intergenic | 40.0% | |
| TTCCACCTCTTTAGGCAAAA+GGG | + | Chr1:88226974-88226993 | None:intergenic | 40.0% | |
| TTGAAGCCGTAATCCAAACT+TGG | + | Chr1:88226436-88226455 | None:intergenic | 40.0% | |
| TTGGCACAATGCTACTAAGA+AGG | + | Chr1:88226632-88226651 | None:intergenic | 40.0% | |
| TTTCCACCTCTTTAGGCAAA+AGG | + | Chr1:88226975-88226994 | None:intergenic | 40.0% | |
| ! | CCAAAAGATTGGAGCTTTTG+AGG | - | Chr1:88226936-88226955 | MsG0180005188.01.T01:CDS | 40.0% |
| ! | CCTCAAAAGCTCCAATCTTT+TGG | + | Chr1:88226939-88226958 | None:intergenic | 40.0% |
| ! | CTAGCATTTTCCACCTCTTT+AGG | + | Chr1:88226982-88227001 | None:intergenic | 40.0% |
| ! | TAAGACCCTTTTGCCTAAAG+AGG | - | Chr1:88226966-88226985 | MsG0180005188.01.T01:CDS | 40.0% |
| ! | TCTTGTTTAGGCTTCTGAAG+TGG | + | Chr1:88226115-88226134 | None:intergenic | 40.0% |
| !! | ATTCTCACAGTTGGTGTCAA+TGG | - | Chr1:88226727-88226746 | MsG0180005188.01.T01:CDS | 40.0% |
| !! | GCTAGGGTTCAAGTTGAAAA+TGG | - | Chr1:88226997-88227016 | MsG0180005188.01.T01:CDS | 40.0% |
| !! | TCCATCAAGGTTTTGTGAGA+AGG | - | Chr1:88226759-88226778 | MsG0180005188.01.T01:CDS | 40.0% |
| ACCTGGTGAGGAATGTGATA+AGG | - | Chr1:88227128-88227147 | MsG0180005188.01.T01:CDS | 45.0% | |
| ACCTTATCACATTCCTCACC+AGG | + | Chr1:88227132-88227151 | None:intergenic | 45.0% | |
| ACGCAACACAGATGATTGAG+AGG | - | Chr1:88226194-88226213 | MsG0180005188.01.T01:CDS | 45.0% | |
| AGGAATATGGCACTTCATGG+AGG | - | Chr1:88226259-88226278 | MsG0180005188.01.T01:CDS | 45.0% | |
| ATACACCAATCGGTGTACCT+TGG | + | Chr1:88226291-88226310 | None:intergenic | 45.0% | |
| ATGGTGAGTTGCATCCATCA+AGG | - | Chr1:88226746-88226765 | MsG0180005188.01.T01:CDS | 45.0% | |
| CGCAACACAGATGATTGAGA+GGG | - | Chr1:88226195-88226214 | MsG0180005188.01.T01:CDS | 45.0% | |
| CGTTTAGCCATTGCTTCCAT+TGG | - | Chr1:88226325-88226344 | MsG0180005188.01.T01:CDS | 45.0% | |
| CTTTGAACATGAGTGGTGAC+AGG | + | Chr1:88226514-88226533 | None:intergenic | 45.0% | |
| GAAAATTAGGTCACCTGGTG+AGG | - | Chr1:88227116-88227135 | MsG0180005188.01.T01:CDS | 45.0% | |
| GCACAATGCTACTAAGAAGG+TGG | + | Chr1:88226629-88226648 | None:intergenic | 45.0% | |
| GCCAAGAGAATTCTCACAGT+TGG | - | Chr1:88226718-88226737 | MsG0180005188.01.T01:CDS | 45.0% | |
| GGTGAGAAAATTAGGTCACC+TGG | - | Chr1:88227111-88227130 | MsG0180005188.01.T01:CDS | 45.0% | |
| GTCTCAGCTTCTGCATTAGA+GGG | + | Chr1:88226852-88226871 | None:intergenic | 45.0% | |
| TGTCTCAGCTTCTGCATTAG+AGG | + | Chr1:88226853-88226872 | None:intergenic | 45.0% | |
| TTAGGGACAACTTTGCTGAC+AGG | - | Chr1:88227090-88227109 | MsG0180005188.01.T01:CDS | 45.0% | |
| ! | GTTTTCACAGCACTGTGTGA+TGG | - | Chr1:88227150-88227169 | MsG0180005188.01.T01:CDS | 45.0% |
| ! | TACACCGATTGGTGTATCGA+TGG | - | Chr1:88226294-88226313 | MsG0180005188.01.T01:CDS | 45.0% |
| CGGCTAGCAGTGAGATTTGA+AGG | + | Chr1:88226409-88226428 | None:intergenic | 50.0% | |
| TGCTAGCCGCAATCCAAGTT+TGG | - | Chr1:88226420-88226439 | MsG0180005188.01.T01:CDS | 50.0% | |
| ! | AGAGGGTATGTAGCACTGCA+AGG | + | Chr1:88226835-88226854 | None:intergenic | 50.0% |
| ! | GACCCTTTTGCCTAAAGAGG+TGG | - | Chr1:88226969-88226988 | MsG0180005188.01.T01:CDS | 50.0% |
| ! | GAGGGTATGTAGCACTGCAA+GGG | + | Chr1:88226834-88226853 | None:intergenic | 50.0% |
| ! | GAGTGGTGACAGGATTAGCA+AGG | + | Chr1:88226504-88226523 | None:intergenic | 50.0% |
| ! | TCACAGCACTGTGTGATGGA+AGG | - | Chr1:88227154-88227173 | MsG0180005188.01.T01:CDS | 50.0% |
| CGATTCGAACATCACCGCAG+TGG | - | Chr1:88226143-88226162 | MsG0180005188.01.T01:CDS | 55.0% | |
| GCAGCAGCCTCAATTTGACC+AGG | + | Chr1:88226025-88226044 | None:intergenic | 55.0% | |
| ATCTGCGGTCCAAGCCACTG+CGG | + | Chr1:88226160-88226179 | None:intergenic | 60.0% | |
| CGAACATCACCGCAGTGGCT+TGG | - | Chr1:88226148-88226167 | MsG0180005188.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 88225968 | 88227239 | 88225968 | ID=MsG0180005188.01;Name=MsG0180005188.01 |
| Chr1 | mRNA | 88225968 | 88227239 | 88225968 | ID=MsG0180005188.01.T01;Parent=MsG0180005188.01;Name=MsG0180005188.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|423 |
| Chr1 | exon | 88225968 | 88227239 | 88225968 | ID=MsG0180005188.01.T01:exon:37162;Parent=MsG0180005188.01.T01 |
| Chr1 | CDS | 88225968 | 88227239 | 88225968 | ID=MsG0180005188.01.T01:cds;Parent=MsG0180005188.01.T01 |
| Gene Sequence |
| Protein sequence |
