AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007221.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007221.01.T01 MTR_2g022850 77.665 197 12 1 3 199 84 248 8.58e-102 294
MsG0280007221.01.T01 MTR_2g084540 74.500 200 19 1 1 200 1 168 4.67e-101 289
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007221.01.T01 AT2G39445 53.061 196 59 2 5 199 17 180 2.11e-63 194
MsG0280007221.01.T01 AT2G39445 53.061 196 59 2 5 199 17 180 2.11e-63 194
MsG0280007221.01.T01 AT1G61280 49.682 157 46 2 43 198 9 133 4.80e-48 154
MsG0280007221.01.T01 AT1G61280 49.682 157 46 2 43 198 9 133 4.80e-48 154
MsG0280007221.01.T01 AT2G39445 52.229 157 43 1 5 161 17 141 5.28e-46 149

Find 67 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTTAAACACAGTTTA+TGG 0.108911 2:+12142593 MsG0280007221.01.T01:CDS
CTCACCATAAACTGTGTTTA+AGG 0.156985 2:-12142597 None:intergenic
CCTAAGCCTTCTGAAGTTTA+CGG 0.308086 2:+12140966 MsG0280007221.01.T01:CDS
GATAGACTCAGTGTTCTTCT+TGG 0.315045 2:-12140861 None:intergenic
TTTCTTCAGATGAGTACAAT+AGG 0.329741 2:+12142765 MsG0280007221.01.T01:intron
ATAGAATGTAGCCATGATTC+TGG 0.349533 2:-12142214 None:intergenic
TGAACATAGCATCATTGATT+CGG 0.411452 2:-12142862 None:intergenic
GAATCATGGCTACATTCTAT+TGG 0.420189 2:+12142217 MsG0280007221.01.T01:CDS
AACATCAGATATAGGATCAA+TGG 0.423073 2:-12142831 None:intergenic
GACGGTGGAGAGCACGGCGG+AGG 0.424743 2:-12139949 None:intergenic
TTCTTCAGATGAGTACAATA+GGG 0.425667 2:+12142766 MsG0280007221.01.T01:intron
CCGTAAACTTCAGAAGGCTT+AGG 0.435785 2:-12140966 None:intergenic
CCAGAGTAGGAGGGTGGTGG+TGG 0.436597 2:-12139971 None:intergenic
GCGAATCTGCTTTCTGCCTG+TGG 0.436679 2:+12139910 MsG0280007221.01.T01:five_prime_UTR
ATTATGTTAGTTGTAATTGA+AGG 0.437914 2:+12140803 MsG0280007221.01.T01:five_prime_UTR
CCACCACCACCCTCCTACTC+TGG 0.440267 2:+12139971 MsG0280007221.01.T01:five_prime_UTR
CCGATACCAACATCAGATAT+AGG 0.443774 2:-12142839 None:intergenic
TAATTGAAGGGGTGCAAGAT+GGG 0.444206 2:+12140816 MsG0280007221.01.T01:exon
ATAACTACCGTTGTTGCTAC+AGG 0.455379 2:+12141002 MsG0280007221.01.T01:CDS
TGGAGAGCACGGCGGAGGGC+GGG 0.463960 2:-12139944 None:intergenic
GTATAACCTGCTAGGATAGT+AGG 0.471940 2:-12142245 None:intergenic
AGGAGGGTGGTGGTGGACGG+TGG 0.478017 2:-12139964 None:intergenic
TCTGGTTTGTCAGTGGATCA+TGG 0.479463 2:+12140942 MsG0280007221.01.T01:CDS
AGTAGGAGGGTGGTGGTGGA+CGG 0.488549 2:-12139967 None:intergenic
TCTGAAGTTTACGGTTTCGT+TGG 0.489982 2:+12140975 MsG0280007221.01.T01:CDS
CGAATCTGCTTTCTGCCTGT+GGG 0.490593 2:+12139911 MsG0280007221.01.T01:five_prime_UTR
ACATCAGATATAGGATCAAT+GGG 0.496284 2:-12142830 None:intergenic
AGGCCAGAGTAGGAGGGTGG+TGG 0.502804 2:-12139974 None:intergenic
CTCTGCCTCTGGTTTGTCAG+TGG 0.505875 2:+12140935 MsG0280007221.01.T01:CDS
TAAACTGTGTTTAAGGATGA+AGG 0.506981 2:-12142590 None:intergenic
GTGGTGGACGGTGGAGAGCA+CGG 0.514605 2:-12139955 None:intergenic
TCTGTCGAGGCCAGAGTAGG+AGG 0.517454 2:-12139981 None:intergenic
CGTAAACTTCAGAAGGCTTA+GGG 0.517726 2:-12140965 None:intergenic
GGGCATATGTTCCAGAATCA+TGG 0.519710 2:+12142203 MsG0280007221.01.T01:CDS
TTATGTTAGTTGTAATTGAA+GGG 0.521959 2:+12140804 MsG0280007221.01.T01:five_prime_UTR
AGAAAGCAGATTCGCGCGCG+AGG 0.527306 2:-12139902 None:intergenic
CCTATATCTGATGTTGGTAT+CGG 0.535443 2:+12142839 MsG0280007221.01.T01:CDS
AAAGCTTCAAATGATAGAGC+AGG 0.542259 2:-12142277 None:intergenic
GTAATTGAAGGGGTGCAAGA+TGG 0.543493 2:+12140815 MsG0280007221.01.T01:exon
ATGGTCACCATCACATAAGT+TGG 0.543794 2:-12142515 None:intergenic
ATTGATCCTATATCTGATGT+TGG 0.555402 2:+12142833 MsG0280007221.01.T01:CDS
GTGGAGAGCACGGCGGAGGG+CGG 0.556363 2:-12139945 None:intergenic
GCATCTCCTACTATCCTAGC+AGG 0.558466 2:+12142239 MsG0280007221.01.T01:CDS
ATGGTGTTGACATGAAGTTA+AGG 0.563140 2:-12142565 None:intergenic
GAAAGCAGATTCGCGCGCGA+GGG 0.570057 2:-12139901 None:intergenic
TATGTTAGTTGTAATTGAAG+GGG 0.570928 2:+12140805 MsG0280007221.01.T01:five_prime_UTR
ACGGTGGAGAGCACGGCGGA+GGG 0.573247 2:-12139948 None:intergenic
ATTGAGGTGTATAACCTGCT+AGG 0.574388 2:-12142253 None:intergenic
CAAACAATTACAAGCAGTAG+AGG 0.576255 2:-12142315 None:intergenic
AAATGATAGAGCAGGAATTG+AGG 0.576868 2:-12142269 None:intergenic
TTGGATACGCCACTGCGCAC+AGG 0.580343 2:+12140778 MsG0280007221.01.T01:five_prime_UTR
GGTGACCATTGTATTAATGT+TGG 0.584804 2:+12142529 MsG0280007221.01.T01:CDS
CACTGAGTCTATCGAGAAAG+CGG 0.586630 2:+12140871 MsG0280007221.01.T01:CDS
CTGTCGAGGCCAGAGTAGGA+GGG 0.594087 2:-12139980 None:intergenic
ACGAAACCGTAAACTTCAGA+AGG 0.604911 2:-12140972 None:intergenic
GCGGGACAGAGAGAACCCAC+AGG 0.608582 2:-12139926 None:intergenic
GAATGCCAACATTAATACAA+TGG 0.613165 2:-12142534 None:intergenic
GAGTCTATCGAGAAAGCGGA+GGG 0.614533 2:+12140875 MsG0280007221.01.T01:CDS
CTTGGACTGCTGACAGAGCG+AGG 0.623030 2:-12140843 None:intergenic
TGAGTCTATCGAGAAAGCGG+AGG 0.646259 2:+12140874 MsG0280007221.01.T01:CDS
GGCAGTACCAACTTATGTGA+TGG 0.650280 2:+12142508 MsG0280007221.01.T01:CDS
TCGAGGCCAGAGTAGGAGGG+TGG 0.651315 2:-12139977 None:intergenic
GTGTTTAAGGATGAAGGAGA+TGG 0.660582 2:-12142584 None:intergenic
ACATAATATCCTGTGCGCAG+TGG 0.669214 2:-12140787 None:intergenic
CAAGCAGTAGAGGATCTGCA+TGG 0.699571 2:-12142305 None:intergenic
ATGATCCACTGACAAACCAG+AGG 0.723474 2:-12140940 None:intergenic
GTGGACGGTGGAGAGCACGG+CGG 0.762260 2:-12139952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTAATATATTATTT+AGG + Chr2:12140283-12140302 MsG0280007221.01.T01:intron 0.0%
!!! ATTTTTTAATATATTATTTA+GGG + Chr2:12140284-12140303 MsG0280007221.01.T01:intron 0.0%
!! ATGCAATTTATTTATTTATT+AGG + Chr2:12140234-12140253 MsG0280007221.01.T01:intron 10.0%
!! ATTTGCATTTAAAAAAAATA+TGG + Chr2:12140706-12140725 MsG0280007221.01.T01:intron 10.0%
!! ATTTGTACATAATTAAATTA+AGG - Chr2:12142400-12142419 None:intergenic 10.0%
!!! AAAAAAAAAATCAACTTTTA+AGG - Chr2:12141935-12141954 None:intergenic 10.0%
!!! ATTTTGAGTTTATTTTAATT+GGG + Chr2:12141444-12141463 MsG0280007221.01.T01:intron 10.0%
!!! ATTTTTTATTTGTTCAAATT+CGG + Chr2:12141291-12141310 MsG0280007221.01.T01:intron 10.0%
!!! TACATAATTTTTTAGAAATT+CGG + Chr2:12142625-12142644 MsG0280007221.01.T01:intron 10.0%
!!! TTTAATCTGTATAAAATTTT+TGG + Chr2:12140348-12140367 MsG0280007221.01.T01:intron 10.0%
!! ATTTCAAATAGTATTTGTAA+TGG + Chr2:12141113-12141132 MsG0280007221.01.T01:intron 15.0%
!! TGAAAAATTGATATATTTAC+AGG - Chr2:12140327-12140346 None:intergenic 15.0%
!!! ATTTGTTTATTTCTGTTTTT+AGG + Chr2:12140507-12140526 MsG0280007221.01.T01:intron 15.0%
!!! GATTTTGAGTTTATTTTAAT+TGG + Chr2:12141443-12141462 MsG0280007221.01.T01:intron 15.0%
!!! TGTTATTTATCTTCAAAATT+GGG + Chr2:12140079-12140098 MsG0280007221.01.T01:intron 15.0%
!!! TTGTTATTTATCTTCAAAAT+TGG + Chr2:12140078-12140097 MsG0280007221.01.T01:intron 15.0%
!!! TTTAGGGTTAAATATATTTT+TGG + Chr2:12140300-12140319 MsG0280007221.01.T01:intron 15.0%
!! AAGAAAATAACTATTCACAA+AGG - Chr2:12142178-12142197 None:intergenic 20.0%
!! AATGTATCTAGTCTATAATA+AGG - Chr2:12141821-12141840 None:intergenic 20.0%
!! ATTATGTACAAATGTAAACT+CGG + Chr2:12142407-12142426 MsG0280007221.01.T01:intron 20.0%
!! ATTATGTTAGTTGTAATTGA+AGG + Chr2:12140803-12140822 MsG0280007221.01.T01:five_prime_UTR 20.0%
!! TAACTAAATATATCGAAAGT+TGG - Chr2:12141703-12141722 None:intergenic 20.0%
!! TATTCAATGTATCTAAAAAG+TGG - Chr2:12141784-12141803 None:intergenic 20.0%
!! TATTTAGTTAATCTATTTCG+TGG + Chr2:12141713-12141732 MsG0280007221.01.T01:intron 20.0%
!! TTATGTTAGTTGTAATTGAA+GGG + Chr2:12140804-12140823 MsG0280007221.01.T01:five_prime_UTR 20.0%
!!! AATAGTTATTTTCTTGGTAT+GGG + Chr2:12142183-12142202 MsG0280007221.01.T01:intron 20.0%
!!! AATTTAGGGTTTTTGATTTA+GGG + Chr2:12140052-12140071 MsG0280007221.01.T01:intron 20.0%
!!! ATTTGTTGTTTTAGTTGTTT+CGG + Chr2:12140581-12140600 MsG0280007221.01.T01:intron 20.0%
!!! CTTTTTGTTAATTGATTATC+AGG + Chr2:12140197-12140216 MsG0280007221.01.T01:intron 20.0%
!!! TTATTTTAATTGGGAACTTA+GGG + Chr2:12141453-12141472 MsG0280007221.01.T01:intron 20.0%
!!! TTTATTTTAATTGGGAACTT+AGG + Chr2:12141452-12141471 MsG0280007221.01.T01:intron 20.0%
!!! TTTGTGAATAGTTATTTTCT+TGG + Chr2:12142177-12142196 MsG0280007221.01.T01:intron 20.0%
! AAACTCAAAATCTCAAACAA+AGG - Chr2:12141435-12141454 None:intergenic 25.0%
! AATTCCAAAAAGTCATTACA+AGG - Chr2:12140632-12140651 None:intergenic 25.0%
! CAAGCACTTATATTAGTATT+TGG + Chr2:12141527-12141546 MsG0280007221.01.T01:intron 25.0%
! GTTATAACATGATTGTATAG+CGG - Chr2:12141209-12141228 None:intergenic 25.0%
! TAATTAGAGTAAAAACGACT+AGG + Chr2:12141957-12141976 MsG0280007221.01.T01:intron 25.0%
! TATGTTAGTTGTAATTGAAG+GGG + Chr2:12140805-12140824 MsG0280007221.01.T01:five_prime_UTR 25.0%
! TTGTACTAAATAGTGTATTG+TGG + Chr2:12141258-12141277 MsG0280007221.01.T01:intron 25.0%
!! ACTTATACTTTTCAGATACT+GGG + Chr2:12142481-12142500 MsG0280007221.01.T01:CDS 25.0%
!! TACTTATACTTTTCAGATAC+TGG + Chr2:12142480-12142499 MsG0280007221.01.T01:CDS 25.0%
!! TTGGGAATTTTGTCAATTTA+GGG + Chr2:12140038-12140057 MsG0280007221.01.T01:intron 25.0%
!! TTTGGGAATTTTGTCAATTT+AGG + Chr2:12140037-12140056 MsG0280007221.01.T01:intron 25.0%
!!! ATTTAGGGTTTTTGATTTAG+GGG + Chr2:12140053-12140072 MsG0280007221.01.T01:intron 25.0%
!!! CAATTTAGGGTTTTTGATTT+AGG + Chr2:12140051-12140070 MsG0280007221.01.T01:intron 25.0%
!!! GAATAGTTATTTTCTTGGTA+TGG + Chr2:12142182-12142201 MsG0280007221.01.T01:intron 25.0%
!!! TGTATAAAATTTTTGGTTCC+TGG + Chr2:12140355-12140374 MsG0280007221.01.T01:intron 25.0%
!!! TGTTTTTAGGCTTTTGAATT+TGG + Chr2:12140520-12140539 MsG0280007221.01.T01:intron 25.0%
!!! TTTGTTATTTTCTCTGTTGT+GGG + Chr2:12140393-12140412 MsG0280007221.01.T01:intron 25.0%
!!! TTTTGTTATTTTCTCTGTTG+TGG + Chr2:12140392-12140411 MsG0280007221.01.T01:intron 25.0%
AAAAAAACCTGTAGCAACAA+CGG - Chr2:12141012-12141031 None:intergenic 30.0%
AAACAACATGTAATGAATCC+AGG + Chr2:12140005-12140024 MsG0280007221.01.T01:intron 30.0%
AACAACATGTAATGAATCCA+GGG + Chr2:12140006-12140025 MsG0280007221.01.T01:intron 30.0%
AACATCAGATATAGGATCAA+TGG - Chr2:12142834-12142853 None:intergenic 30.0%
ACATCAGATATAGGATCAAT+GGG - Chr2:12142833-12142852 None:intergenic 30.0%
ACTCTGATCCAAAAATATTG+GGG - Chr2:12140757-12140776 None:intergenic 30.0%
AGACTCTGATCCAAAAATAT+TGG - Chr2:12140759-12140778 None:intergenic 30.0%
ATTGATCCTATATCTGATGT+TGG + Chr2:12142833-12142852 MsG0280007221.01.T01:CDS 30.0%
ATTTGTGCAATTATAACACC+AGG - Chr2:12141902-12141921 None:intergenic 30.0%
GAAAATCAGAGATGTTAAGT+AGG - Chr2:12142009-12142028 None:intergenic 30.0%
GAATGCCAACATTAATACAA+TGG - Chr2:12142537-12142556 None:intergenic 30.0%
GACTCTGATCCAAAAATATT+GGG - Chr2:12140758-12140777 None:intergenic 30.0%
TAAACTGTGTTTAAGGATGA+AGG - Chr2:12142593-12142612 None:intergenic 30.0%
TACAAATGTAAACTCGGTTA+AGG + Chr2:12142413-12142432 MsG0280007221.01.T01:intron 30.0%
TCATCCTTAAACACAGTTTA+TGG + Chr2:12142593-12142612 MsG0280007221.01.T01:CDS 30.0%
TCCAAAAAATCAAGACTCAA+AGG - Chr2:12142794-12142813 None:intergenic 30.0%
TTCTTCAGATGAGTACAATA+GGG + Chr2:12142766-12142785 MsG0280007221.01.T01:intron 30.0%
TTGTTAAATAGCAGCTATAG+CGG - Chr2:12141338-12141357 None:intergenic 30.0%
TTTCTTCAGATGAGTACAAT+AGG + Chr2:12142765-12142784 MsG0280007221.01.T01:intron 30.0%
! CAGATGAAAGAATCACATTT+TGG - Chr2:12141678-12141697 None:intergenic 30.0%
! CTTTTGAATTTGGATTTAGC+GGG + Chr2:12140530-12140549 MsG0280007221.01.T01:intron 30.0%
! GCTTTTGAATTTGGATTTAG+CGG + Chr2:12140529-12140548 MsG0280007221.01.T01:intron 30.0%
! GTTTAAGCTTTCTTTTTGTG+TGG + Chr2:12141395-12141414 MsG0280007221.01.T01:intron 30.0%
! GTTTTGCTTATAATAGTGAC+CGG + Chr2:12141868-12141887 MsG0280007221.01.T01:intron 30.0%
! TGAACATAGCATCATTGATT+CGG - Chr2:12142865-12142884 None:intergenic 30.0%
!! ATGACTCATAAGCTATTTTG+TGG - Chr2:12141616-12141635 None:intergenic 30.0%
!! GTTGTTGATTTGCTCAAAAT+TGG + Chr2:12140141-12140160 MsG0280007221.01.T01:intron 30.0%
!!! AGTCTTGATTTTTTGGAAGA+CGG + Chr2:12142797-12142816 MsG0280007221.01.T01:CDS 30.0%
!!! ATATTTTTGGATCAGAGTCT+TGG + Chr2:12140759-12140778 MsG0280007221.01.T01:intron 30.0%
!!! GGCAGAGTTGTTTTTATTAT+CGG - Chr2:12140922-12140941 None:intergenic 30.0%
!!! TCCTTTGAGTCTTGATTTTT+TGG + Chr2:12142790-12142809 MsG0280007221.01.T01:CDS 30.0%
AAAGCTTCAAATGATAGAGC+AGG - Chr2:12142280-12142299 None:intergenic 35.0%
AAATGATAGAGCAGGAATTG+AGG - Chr2:12142272-12142291 None:intergenic 35.0%
AACTTATATGTTACTCCCTC+CGG + Chr2:12141742-12141761 MsG0280007221.01.T01:intron 35.0%
AAGACTGTGTTATCACTTAG+GGG + Chr2:12142688-12142707 MsG0280007221.01.T01:intron 35.0%
AATAAAAACAACTCTGCCTC+TGG + Chr2:12140924-12140943 MsG0280007221.01.T01:CDS 35.0%
AGTAAAAACGACTAGGAACA+GGG + Chr2:12141964-12141983 MsG0280007221.01.T01:intron 35.0%
ATAGAATGTAGCCATGATTC+TGG - Chr2:12142217-12142236 None:intergenic 35.0%
CAAACAATTACAAGCAGTAG+AGG - Chr2:12142318-12142337 None:intergenic 35.0%
CAAGACTGTGTTATCACTTA+GGG + Chr2:12142687-12142706 MsG0280007221.01.T01:intron 35.0%
CCTATATCTGATGTTGGTAT+CGG + Chr2:12142839-12142858 MsG0280007221.01.T01:CDS 35.0%
CTCACCATAAACTGTGTTTA+AGG - Chr2:12142600-12142619 None:intergenic 35.0%
CTCTACTGCTTGTAATTGTT+TGG + Chr2:12142316-12142335 MsG0280007221.01.T01:intron 35.0%
CTCTGATCCAAAAATATTGG+GGG - Chr2:12140756-12140775 None:intergenic 35.0%
GAATCATGGCTACATTCTAT+TGG + Chr2:12142217-12142236 MsG0280007221.01.T01:CDS 35.0%
GGTGACCATTGTATTAATGT+TGG + Chr2:12142529-12142548 MsG0280007221.01.T01:CDS 35.0%
TAGAGACATTCGTTAACACA+TGG - Chr2:12142099-12142118 None:intergenic 35.0%
TGTGTTAACGAATGTCTCTA+AGG + Chr2:12142099-12142118 MsG0280007221.01.T01:intron 35.0%
TTAAGTAGGATGTGTCTTCA+AGG - Chr2:12141995-12142014 None:intergenic 35.0%
TTGTCTAATAGCTGCTATAG+CGG - Chr2:12141234-12141253 None:intergenic 35.0%
! AAACAGTATAGCTTGAGTTG+AGG + Chr2:12141168-12141187 MsG0280007221.01.T01:intron 35.0%
! TGGAATTTTGCTTAAGTGAC+CGG - Chr2:12141764-12141783 None:intergenic 35.0%
!! AATCCAGGGTTTTGTGATTT+GGG + Chr2:12140020-12140039 MsG0280007221.01.T01:intron 35.0%
!! ATGGTGTTGACATGAAGTTA+AGG - Chr2:12142568-12142587 None:intergenic 35.0%
!! GAGCTGATTTTGAGCTTATT+AGG + Chr2:12141487-12141506 MsG0280007221.01.T01:intron 35.0%
!!! GTCTTGATTTTTTGGAAGAC+GGG + Chr2:12142798-12142817 MsG0280007221.01.T01:CDS 35.0%
!!! TACATGTTGTTTTTCTGTCG+AGG - Chr2:12139997-12140016 None:intergenic 35.0%
!!! TCTTGATTTTTTGGAAGACG+GGG + Chr2:12142799-12142818 MsG0280007221.01.T01:CDS 35.0%
!!! TGTTGTTTTAGTTGTTTCGG+TGG + Chr2:12140584-12140603 MsG0280007221.01.T01:intron 35.0%
!!! TTGGCCTTGTAATGACTTTT+TGG + Chr2:12140625-12140644 MsG0280007221.01.T01:intron 35.0%
AAAAAAGCAGAAGCTGAACC+AGG - Chr2:12140376-12140395 None:intergenic 40.0%
ACGAAACCGTAAACTTCAGA+AGG - Chr2:12140975-12140994 None:intergenic 40.0%
ATAACTACCGTTGTTGCTAC+AGG + Chr2:12141002-12141021 MsG0280007221.01.T01:CDS 40.0%
ATGGTCACCATCACATAAGT+TGG - Chr2:12142518-12142537 None:intergenic 40.0%
ATTCCCAAATCACAAAACCC+TGG - Chr2:12140026-12140045 None:intergenic 40.0%
ATTGAGGTGTATAACCTGCT+AGG - Chr2:12142256-12142275 None:intergenic 40.0%
CCGATACCAACATCAGATAT+AGG - Chr2:12142842-12142861 None:intergenic 40.0%
CGTAAACTTCAGAAGGCTTA+GGG - Chr2:12140968-12140987 None:intergenic 40.0%
GAGTAAAAACGACTAGGAAC+AGG + Chr2:12141963-12141982 MsG0280007221.01.T01:intron 40.0%
GATAGACTCAGTGTTCTTCT+TGG - Chr2:12140864-12140883 None:intergenic 40.0%
GCAAGACTGTGTTATCACTT+AGG + Chr2:12142686-12142705 MsG0280007221.01.T01:intron 40.0%
GTATAACCTGCTAGGATAGT+AGG - Chr2:12142248-12142267 None:intergenic 40.0%
GTGTTTAAGGATGAAGGAGA+TGG - Chr2:12142587-12142606 None:intergenic 40.0%
TGAATTTGGATTTAGCGGGA+AGG + Chr2:12140534-12140553 MsG0280007221.01.T01:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + Chr2:12141872-12141891 MsG0280007221.01.T01:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + Chr2:12141871-12141890 MsG0280007221.01.T01:intron 40.0%
! AATTTTGCTTAAGTGACCGG+AGG - Chr2:12141761-12141780 None:intergenic 40.0%
! ACTTTTCAGATACTGGGCTT+TGG + Chr2:12142487-12142506 MsG0280007221.01.T01:CDS 40.0%
! AGCTTTCTTTTTGTGTGGCA+TGG + Chr2:12141400-12141419 MsG0280007221.01.T01:intron 40.0%
! ATTTTGCTTAAGTGACCGGA+GGG - Chr2:12141760-12141779 None:intergenic 40.0%
! GATCGATCCCCCAATATTTT+TGG + Chr2:12140746-12140765 MsG0280007221.01.T01:intron 40.0%
! GTTGGTATTCTGTGGCATTT+TGG + Chr2:12140606-12140625 MsG0280007221.01.T01:intron 40.0%
! TAATTGAAGGGGTGCAAGAT+GGG + Chr2:12140816-12140835 MsG0280007221.01.T01:exon 40.0%
! TCTGAAGTTTACGGTTTCGT+TGG + Chr2:12140975-12140994 MsG0280007221.01.T01:CDS 40.0%
! TCTTTTTGTGTGGCATGGTA+GGG + Chr2:12141405-12141424 MsG0280007221.01.T01:intron 40.0%
! TTCTTTTTGTGTGGCATGGT+AGG + Chr2:12141404-12141423 MsG0280007221.01.T01:intron 40.0%
!! CCTAAGCCTTCTGAAGTTTA+CGG + Chr2:12140966-12140985 MsG0280007221.01.T01:CDS 40.0%
!! GAATCCAGGGTTTTGTGATT+TGG + Chr2:12140019-12140038 MsG0280007221.01.T01:intron 40.0%
!!! GTTTTAGTTGTTTCGGTGGT+TGG + Chr2:12140588-12140607 MsG0280007221.01.T01:intron 40.0%
ACATAATATCCTGTGCGCAG+TGG - Chr2:12140790-12140809 None:intergenic 45.0%
ACTGTGTTATCACTTAGGGG+AGG + Chr2:12142691-12142710 MsG0280007221.01.T01:intron 45.0%
ATGATCCACTGACAAACCAG+AGG - Chr2:12140943-12140962 None:intergenic 45.0%
CACTGAGTCTATCGAGAAAG+CGG + Chr2:12140871-12140890 MsG0280007221.01.T01:CDS 45.0%
CATAAAATGCCGAGCATCAC+AGG - Chr2:12142128-12142147 None:intergenic 45.0%
CCGTAAACTTCAGAAGGCTT+AGG - Chr2:12140969-12140988 None:intergenic 45.0%
CTAAGGTTTCCTGTGATGCT+CGG + Chr2:12142116-12142135 MsG0280007221.01.T01:intron 45.0%
CTGTGTTATCACTTAGGGGA+GGG + Chr2:12142692-12142711 MsG0280007221.01.T01:intron 45.0%
GGCAGTACCAACTTATGTGA+TGG + Chr2:12142508-12142527 MsG0280007221.01.T01:CDS 45.0%
GGGCATATGTTCCAGAATCA+TGG + Chr2:12142203-12142222 MsG0280007221.01.T01:CDS 45.0%
TACGTTGGATAGCCATGTTG+TGG + Chr2:12140427-12140446 MsG0280007221.01.T01:intron 45.0%
! CTTTTTGTGTGGCATGGTAG+GGG + Chr2:12141406-12141425 MsG0280007221.01.T01:intron 45.0%
! GGATTTAGCGGGAAGGTTTT+TGG + Chr2:12140541-12140560 MsG0280007221.01.T01:intron 45.0%
! GTAATTGAAGGGGTGCAAGA+TGG + Chr2:12140815-12140834 MsG0280007221.01.T01:exon 45.0%
! TCTGGTTTGTCAGTGGATCA+TGG + Chr2:12140942-12140961 MsG0280007221.01.T01:CDS 45.0%
! TTTCGGTGGTTGGTATTCTG+TGG + Chr2:12140598-12140617 MsG0280007221.01.T01:intron 45.0%
ACTGCAGGGTTCTGCATATG+CGG + Chr2:12140457-12140476 MsG0280007221.01.T01:intron 50.0%
ATAACACCAGGTACTCCCTC+CGG - Chr2:12141890-12141909 None:intergenic 50.0%
CAAGCAGTAGAGGATCTGCA+TGG - Chr2:12142308-12142327 None:intergenic 50.0%
CGAATCTGCTTTCTGCCTGT+GGG + Chr2:12139911-12139930 MsG0280007221.01.T01:five_prime_UTR 50.0%
GAGTCTATCGAGAAAGCGGA+GGG + Chr2:12140875-12140894 MsG0280007221.01.T01:CDS 50.0%
GCATCTCCTACTATCCTAGC+AGG + Chr2:12142239-12142258 MsG0280007221.01.T01:CDS 50.0%
GTTGTGGCTGCAAAACTGCA+GGG + Chr2:12140443-12140462 MsG0280007221.01.T01:intron 50.0%
TGAGTCTATCGAGAAAGCGG+AGG + Chr2:12140874-12140893 MsG0280007221.01.T01:CDS 50.0%
TGTTGTGGCTGCAAAACTGC+AGG + Chr2:12140442-12140461 MsG0280007221.01.T01:intron 50.0%
! GCAGTTTTGCAGCCACAACA+TGG - Chr2:12140442-12140461 None:intergenic 50.0%
! TGGGCGTCTGTTTGTTACGT+TGG + Chr2:12140412-12140431 MsG0280007221.01.T01:intron 50.0%
! TTTTCTGTCGAGGCCAGAGT+AGG - Chr2:12139987-12140006 None:intergenic 50.0%
CTGCAGGGTTCTGCATATGC+GGG + Chr2:12140458-12140477 MsG0280007221.01.T01:intron 55.0%
GCGAATCTGCTTTCTGCCTG+TGG + Chr2:12139910-12139929 MsG0280007221.01.T01:five_prime_UTR 55.0%
! CTCTGCCTCTGGTTTGTCAG+TGG + Chr2:12140935-12140954 MsG0280007221.01.T01:CDS 55.0%
AGAAAGCAGATTCGCGCGCG+AGG - Chr2:12139905-12139924 None:intergenic 60.0%
CTGTCGAGGCCAGAGTAGGA+GGG - Chr2:12139983-12140002 None:intergenic 60.0%
CTTGGACTGCTGACAGAGCG+AGG - Chr2:12140846-12140865 None:intergenic 60.0%
GAAAGCAGATTCGCGCGCGA+GGG - Chr2:12139904-12139923 None:intergenic 60.0%
TCTGTCGAGGCCAGAGTAGG+AGG - Chr2:12139984-12140003 None:intergenic 60.0%
TTGGATACGCCACTGCGCAC+AGG + Chr2:12140778-12140797 MsG0280007221.01.T01:five_prime_UTR 60.0%
!! AGTAGGAGGGTGGTGGTGGA+CGG - Chr2:12139970-12139989 None:intergenic 60.0%
!! TAGTGACCGGAGGGAGTACC+TGG + Chr2:12141881-12141900 MsG0280007221.01.T01:intron 60.0%
AGGCCAGAGTAGGAGGGTGG+TGG - Chr2:12139977-12139996 None:intergenic 65.0%
CCACCACCACCCTCCTACTC+TGG + Chr2:12139971-12139990 MsG0280007221.01.T01:five_prime_UTR 65.0%
GCGGGACAGAGAGAACCCAC+AGG - Chr2:12139929-12139948 None:intergenic 65.0%
TCGAGGCCAGAGTAGGAGGG+TGG - Chr2:12139980-12139999 None:intergenic 65.0%
! CCAGAGTAGGAGGGTGGTGG+TGG - Chr2:12139974-12139993 None:intergenic 65.0%
!! GTGGTGGACGGTGGAGAGCA+CGG - Chr2:12139958-12139977 None:intergenic 65.0%
! ACGGTGGAGAGCACGGCGGA+GGG - Chr2:12139951-12139970 None:intergenic 70.0%
! GTGGACGGTGGAGAGCACGG+CGG - Chr2:12139955-12139974 None:intergenic 70.0%
!! AGGAGGGTGGTGGTGGACGG+TGG - Chr2:12139967-12139986 None:intergenic 70.0%
! GACGGTGGAGAGCACGGCGG+AGG - Chr2:12139952-12139971 None:intergenic 75.0%
! GTGGAGAGCACGGCGGAGGG+CGG - Chr2:12139948-12139967 None:intergenic 75.0%
! TGGAGAGCACGGCGGAGGGC+GGG - Chr2:12139947-12139966 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 12139866 12142898 12139866 ID=MsG0280007221.01;Name=MsG0280007221.01
Chr2 mRNA 12139866 12142898 12139866 ID=MsG0280007221.01.T01;Parent=MsG0280007221.01;Name=MsG0280007221.01.T01;_AED=0.45;_eAED=0.47;_QI=208|0.75|0.4|1|0.5|0.4|5|0|200
Chr2 exon 12139866 12140013 12139866 ID=MsG0280007221.01.T01:exon:3657;Parent=MsG0280007221.01.T01
Chr2 exon 12140774 12141023 12140774 ID=MsG0280007221.01.T01:exon:3658;Parent=MsG0280007221.01.T01
Chr2 exon 12142188 12142326 12142188 ID=MsG0280007221.01.T01:exon:3659;Parent=MsG0280007221.01.T01
Chr2 exon 12142466 12142614 12142466 ID=MsG0280007221.01.T01:exon:3660;Parent=MsG0280007221.01.T01
Chr2 exon 12142774 12142898 12142774 ID=MsG0280007221.01.T01:exon:3661;Parent=MsG0280007221.01.T01
Chr2 five_prime_UTR 12139866 12140013 12139866 ID=MsG0280007221.01.T01:five_prime_utr;Parent=MsG0280007221.01.T01
Chr2 five_prime_UTR 12140774 12140833 12140774 ID=MsG0280007221.01.T01:five_prime_utr;Parent=MsG0280007221.01.T01
Chr2 CDS 12140834 12141023 12140834 ID=MsG0280007221.01.T01:cds;Parent=MsG0280007221.01.T01
Chr2 CDS 12142188 12142326 12142188 ID=MsG0280007221.01.T01:cds;Parent=MsG0280007221.01.T01
Chr2 CDS 12142466 12142614 12142466 ID=MsG0280007221.01.T01:cds;Parent=MsG0280007221.01.T01
Chr2 CDS 12142774 12142898 12142774 ID=MsG0280007221.01.T01:cds;Parent=MsG0280007221.01.T01
Gene Sequence

>MsG0280007221.01.T01

ATGGGAAGTCCTCGCTCTGTCAGCAGTCCAAGAAGAACACTGAGTCTATCGAGAAAGCGGAGGGCAACTGTGTCATTTCTTGATTCCGATAATAAAAACAACTCTGCCTCTGGTTTGTCAGTGGATCATGGCCCTAAGCCTTCTGAAGTTTACGGTTTCGTTGGATCTATAACTACCGTTGTTGCTACAGTTATTTTCTTGGTATGGGCATATGTTCCAGAATCATGGCTACATTCTATTGGCATCTCCTACTATCCTAGCAGGTTATACACCTCAATTCCTGCTCTATCATTTGAAGCTTTAGCAACCATGCAGATCCTCTACTGCTTTTTAGCTGTTAAACTACTTATACTTTTCAGATACTGGGCTTTGGCAGTACCAACTTATGTGATGGTGACCATTGTATTAATGTTGGCATTCTACATTAGCCTTAACTTCATGTCAACACCATCTCCTTCATCCTTAAACACAGTTTATGATGAGTACAATAGGGATCCTTTGAGTCTTGATTTTTTGGAAGACGGGGATGAGAAGCCCATTGATCCTATATCTGATGTTGGTATCGGCCGAATCAATGATGCTATGTTCAATAATTCAGCCTAA
Protein sequence

>MsG0280007221.01.T01

MGSPRSVSSPRRTLSLSRKRRATVSFLDSDNKNNSASGLSVDHGPKPSEVYGFVGSITTVVATVIFLVWAYVPESWLHSIGISYYPSRLYTSIPALSFEALATMQILYCFLAVKLLILFRYWALAVPTYVMVTIVLMLAFYISLNFMSTPSPSSLNTVYDEYNRDPLSLDFLEDGDEKPIDPISDVGIGRINDAMFNNSA*