AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007504.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007504.01.T01 MTR_2g026670 100.000 73 0 0 1 73 175 247 8.04e-49 155
MsG0280007504.01.T01 MTR_4g133900 64.103 78 19 3 2 74 185 258 1.05e-22 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGGTCCACCATACTGATT+AGG 0.374070 2:+16384309 None:intergenic
TGGAGGTGGGCCGTGTTATG+TGG 0.393001 2:-16384241 MsG0280007504.01.T01:CDS
TATGGGCCGTATGGCCCAAC+AGG 0.440070 2:+16384333 None:intergenic
CCACCATACTGATTAGGGTA+TGG 0.446251 2:+16384315 None:intergenic
GGAACATAACACATCCCAAC+TGG 0.447009 2:+16384375 None:intergenic
CCACCATAGCTATCGTATAC+TGG 0.450124 2:+16384261 None:intergenic
GGCCCATACCCTAATCAGTA+TGG 0.453969 2:-16384318 MsG0280007504.01.T01:CDS
TCCACAAATGCCACCACCTA+TGG 0.459788 2:-16384403 None:intergenic
CACCTATGGCGGTTCCAGTT+GGG 0.463164 2:-16384389 MsG0280007504.01.T01:CDS
CCACCTATGGCGGTTCCAGT+TGG 0.463360 2:-16384390 MsG0280007504.01.T01:CDS
GGTAGACCTGTTGGGCCATA+CGG 0.476910 2:-16384339 MsG0280007504.01.T01:CDS
TACGATAGCTATGGTGGAGG+TGG 0.483595 2:-16384255 MsG0280007504.01.T01:CDS
GGAGGTGGGCCGTGTTATGT+GGG 0.492576 2:-16384240 MsG0280007504.01.T01:CDS
CTCAGCGAAGAAAACGCAAC+AGG 0.503972 2:-16384201 MsG0280007504.01.T01:CDS
CACCATACTGATTAGGGTAT+GGG 0.504883 2:+16384316 None:intergenic
GTGGTCCACCATACTGATTA+GGG 0.511313 2:+16384310 None:intergenic
GCTACGAGGGTAGACCTGTT+GGG 0.519466 2:-16384347 MsG0280007504.01.T01:CDS
TGCTACGAGGGTAGACCTGT+TGG 0.523238 2:-16384348 MsG0280007504.01.T01:CDS
ACTGGAACCGCCATAGGTGG+TGG 0.525042 2:+16384393 None:intergenic
TGATTAGGGTATGGGCCGTA+TGG 0.546802 2:+16384324 None:intergenic
CGCTGAGATATTGATCGCAT+CGG 0.559500 2:+16384217 None:intergenic
CCAGTATACGATAGCTATGG+TGG 0.563800 2:-16384261 MsG0280007504.01.T01:CDS
ACGATAGCTATGGTGGAGGT+GGG 0.588196 2:-16384254 MsG0280007504.01.T01:CDS
TCGCATCGGCCCACATAACA+CGG 0.600233 2:+16384231 None:intergenic
ATCCCAACTGGAACCGCCAT+AGG 0.607318 2:+16384387 None:intergenic
ACAGGTCTACCCTCGTAGCA+TGG 0.615096 2:+16384351 None:intergenic
AGACCAGTATACGATAGCTA+TGG 0.617281 2:-16384264 MsG0280007504.01.T01:CDS
GGTGGACCACAATGTTATGA+CGG 0.619668 2:-16384297 MsG0280007504.01.T01:CDS
CCAACTGGAACCGCCATAGG+TGG 0.630035 2:+16384390 None:intergenic
ACAAATGCCACCACCTATGG+CGG 0.640749 2:-16384400 None:intergenic
GTATACGATAGCTATGGTGG+AGG 0.658092 2:-16384258 MsG0280007504.01.T01:CDS
CCATACCCTAATCAGTATGG+TGG 0.668874 2:-16384315 MsG0280007504.01.T01:CDS
TAGTATCCGTCATAACATTG+TGG 0.672588 2:+16384291 None:intergenic
TTATGTTCCACCATGCTACG+AGG 0.686372 2:-16384361 MsG0280007504.01.T01:CDS
TATGTTCCACCATGCTACGA+GGG 0.710253 2:-16384360 MsG0280007504.01.T01:CDS
GGTCTACCCTCGTAGCATGG+TGG 0.713983 2:+16384354 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAGTATCCGTCATAACATTG+TGG + Chr2:16384245-16384264 None:intergenic 35.0%
AGACCAGTATACGATAGCTA+TGG - Chr2:16384269-16384288 MsG0280007504.01.T01:CDS 40.0%
! CACCATACTGATTAGGGTAT+GGG + Chr2:16384220-16384239 None:intergenic 40.0%
CCACCATAGCTATCGTATAC+TGG + Chr2:16384275-16384294 None:intergenic 45.0%
CCAGTATACGATAGCTATGG+TGG - Chr2:16384272-16384291 MsG0280007504.01.T01:CDS 45.0%
CCATACCCTAATCAGTATGG+TGG - Chr2:16384218-16384237 MsG0280007504.01.T01:CDS 45.0%
CGCTGAGATATTGATCGCAT+CGG + Chr2:16384319-16384338 None:intergenic 45.0%
GGAACATAACACATCCCAAC+TGG + Chr2:16384161-16384180 None:intergenic 45.0%
GGTGGACCACAATGTTATGA+CGG - Chr2:16384236-16384255 MsG0280007504.01.T01:CDS 45.0%
GTATACGATAGCTATGGTGG+AGG - Chr2:16384275-16384294 MsG0280007504.01.T01:CDS 45.0%
TATGTTCCACCATGCTACGA+GGG - Chr2:16384173-16384192 MsG0280007504.01.T01:CDS 45.0%
TTATGTTCCACCATGCTACG+AGG - Chr2:16384172-16384191 MsG0280007504.01.T01:CDS 45.0%
! CCACCATACTGATTAGGGTA+TGG + Chr2:16384221-16384240 None:intergenic 45.0%
! GTGGTCCACCATACTGATTA+GGG + Chr2:16384226-16384245 None:intergenic 45.0%
! TGTGGTCCACCATACTGATT+AGG + Chr2:16384227-16384246 None:intergenic 45.0%
CTCAGCGAAGAAAACGCAAC+AGG - Chr2:16384332-16384351 MsG0280007504.01.T01:CDS 50.0%
GGCCCATACCCTAATCAGTA+TGG - Chr2:16384215-16384234 MsG0280007504.01.T01:CDS 50.0%
TGATTAGGGTATGGGCCGTA+TGG + Chr2:16384212-16384231 None:intergenic 50.0%
! ACGATAGCTATGGTGGAGGT+GGG - Chr2:16384279-16384298 MsG0280007504.01.T01:CDS 50.0%
! TACGATAGCTATGGTGGAGG+TGG - Chr2:16384278-16384297 MsG0280007504.01.T01:CDS 50.0%
ACAGGTCTACCCTCGTAGCA+TGG + Chr2:16384185-16384204 None:intergenic 55.0%
ATCCCAACTGGAACCGCCAT+AGG + Chr2:16384149-16384168 None:intergenic 55.0%
CACCTATGGCGGTTCCAGTT+GGG - Chr2:16384144-16384163 MsG0280007504.01.T01:CDS 55.0%
GCTACGAGGGTAGACCTGTT+GGG - Chr2:16384186-16384205 MsG0280007504.01.T01:CDS 55.0%
GGTAGACCTGTTGGGCCATA+CGG - Chr2:16384194-16384213 MsG0280007504.01.T01:CDS 55.0%
TGCTACGAGGGTAGACCTGT+TGG - Chr2:16384185-16384204 MsG0280007504.01.T01:CDS 55.0%
! TCGCATCGGCCCACATAACA+CGG + Chr2:16384305-16384324 None:intergenic 55.0%
ACTGGAACCGCCATAGGTGG+TGG + Chr2:16384143-16384162 None:intergenic 60.0%
CCAACTGGAACCGCCATAGG+TGG + Chr2:16384146-16384165 None:intergenic 60.0%
CCACCTATGGCGGTTCCAGT+TGG - Chr2:16384143-16384162 MsG0280007504.01.T01:CDS 60.0%
GGAGGTGGGCCGTGTTATGT+GGG - Chr2:16384293-16384312 MsG0280007504.01.T01:CDS 60.0%
GGTCTACCCTCGTAGCATGG+TGG + Chr2:16384182-16384201 None:intergenic 60.0%
TATGGGCCGTATGGCCCAAC+AGG + Chr2:16384203-16384222 None:intergenic 60.0%
TGGAGGTGGGCCGTGTTATG+TGG - Chr2:16384292-16384311 MsG0280007504.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16384137 16384418 16384137 ID=MsG0280007504.01;Name=MsG0280007504.01
Chr2 mRNA 16384137 16384418 16384137 ID=MsG0280007504.01.T01;Parent=MsG0280007504.01;Name=MsG0280007504.01.T01;_AED=0.46;_eAED=0.56;_QI=0|-1|0|1|-1|1|1|0|93
Chr2 exon 16384137 16384418 16384137 ID=MsG0280007504.01.T01:exon:1234;Parent=MsG0280007504.01.T01
Chr2 CDS 16384137 16384418 16384137 ID=MsG0280007504.01.T01:cds;Parent=MsG0280007504.01.T01
Gene Sequence

>MsG0280007504.01.T01

ATGCCACCACCTATGGCGGTTCCAGTTGGGATGTGTTATGTTCCACCATGCTACGAGGGTAGACCTGTTGGGCCATACGGCCCATACCCTAATCAGTATGGTGGACCACAATGTTATGACGGATACTATGCAAGACCAGTATACGATAGCTATGGTGGAGGTGGGCCGTGTTATGTGGGCCGATGCGATCAATATCTCAGCGAAGAAAACGCAACAGGTGCACATTATGTGATTCTTCATCACATTCACATCACTTTTAGAATTGTTCCTTTGTTTTTATAA

Protein sequence

>MsG0280007504.01.T01

MPPPMAVPVGMCYVPPCYEGRPVGPYGPYPNQYGGPQCYDGYYARPVYDSYGGGGPCYVGRCDQYLSEENATGAHYVILHHIHITFRIVPLFL*