AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003258.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003258.01.T01 MTR_0508s0010 88.462 260 7 1 1 237 1 260 1.71e-166 460
MsG0180003258.01.T01 MTR_3g088865 82.692 260 22 1 1 237 1 260 3.23e-151 421
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003258.01.T01 AT5G06360 78.462 260 33 1 1 237 1 260 2.28e-149 417

Find 90 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACTATTGTTCTATTTATT+TGG 0.108486 1:-59260728 MsG0180003258.01.T01:three_prime_UTR
ACATTCAATCTTGAAATTAT+TGG 0.186081 1:-59261688 MsG0180003258.01.T01:CDS
AGCTGTTCCTGCTTATCTTT+TGG 0.213713 1:-59262670 MsG0180003258.01.T01:CDS
TATAAATCTGTTAGATTCAT+TGG 0.256304 1:+59260656 None:intergenic
TCAACTATTGGAGGCGGCTT+AGG 0.306421 1:+59264269 None:intergenic
GACCGTCACATAAAAGAAAA+TGG 0.314189 1:-59263233 MsG0180003258.01.T01:CDS
AGCCTCTCTCTTGCGCTTGC+GGG 0.318070 1:+59263190 None:intergenic
TTTCTGGGTTGTTGGTAACC+TGG 0.337046 1:+59260962 None:intergenic
GAAACAGACTATTGGTCTTA+AGG 0.346482 1:-59263020 MsG0180003258.01.T01:intron
CAGGTGAATGTCAGCGAACT+TGG 0.351479 1:-59261116 MsG0180003258.01.T01:CDS
GAGGTGTACATGGGGCCATT+AGG 0.355093 1:+59261652 None:intergenic
TTGAATGTGCATTTAAGTTC+AGG 0.355975 1:+59261703 None:intergenic
CCTCTCTTGGTGTCATTACC+AGG 0.375954 1:-59261633 MsG0180003258.01.T01:CDS
AAGCTACTCTCGTTGGAGCC+TGG 0.377046 1:+59264241 None:intergenic
AAAATTGAGTACCTTGTGTT+AGG 0.380219 1:+59264179 None:intergenic
AGCTACTCTCGTTGGAGCCT+GGG 0.385235 1:+59264242 None:intergenic
GGTTCACAACCACTCTAAAA+AGG 0.386198 1:-59263162 MsG0180003258.01.T01:CDS
AGGTGTACATGGGGCCATTA+GGG 0.387835 1:+59261653 None:intergenic
TGTGTTAGGCGGTGGCAGTC+TGG 0.394572 1:+59264193 None:intergenic
AAACAGACTATTGGTCTTAA+GGG 0.408363 1:-59263019 MsG0180003258.01.T01:intron
TTATTACTTCTTGCACTTGA+AGG 0.410300 1:-59260689 MsG0180003258.01.T01:three_prime_UTR
ATTCGAGTGAAACAGACTAT+TGG 0.411760 1:-59263028 MsG0180003258.01.T01:intron
CGAATGAATCGCTCATACTT+TGG 0.413112 1:+59261766 None:intergenic
CAAGGCTGATGATAATGTTC+AGG 0.418037 1:-59262697 MsG0180003258.01.T01:CDS
TTCTGGGTTGTTGGTAACCT+GGG 0.421246 1:+59260963 None:intergenic
AAACTAGTTCAGTTTATAAC+GGG 0.426344 1:+59263456 None:intergenic
CAACTATTGGAGGCGGCTTA+GGG 0.428058 1:+59264270 None:intergenic
GGAAAAGGGTTAAACCTTCT+GGG 0.431363 1:+59263139 None:intergenic
GTTACAAAAGCAACATTTGT+TGG 0.433060 1:-59261811 MsG0180003258.01.T01:CDS
AAAACTAGTTCAGTTTATAA+CGG 0.449796 1:+59263455 None:intergenic
TTACAAAAGCAACATTTGTT+GGG 0.454651 1:-59261810 MsG0180003258.01.T01:CDS
GGCCCCATGTACACCTCTCT+TGG 0.465138 1:-59261646 MsG0180003258.01.T01:CDS
GAGCCTCTCTCTTGCGCTTG+CGG 0.475580 1:+59263189 None:intergenic
TACTACAACATGACAGTGTC+CGG 0.485770 1:+59260750 None:intergenic
AAAGCAACATTTGTTGGGCC+TGG 0.494371 1:-59261805 MsG0180003258.01.T01:CDS
AACATTTCATCTTCAGCCAC+AGG 0.498226 1:+59262301 None:intergenic
GAGCGATTCATTCGTCCCAC+TGG 0.500866 1:-59261757 MsG0180003258.01.T01:CDS
ATGAATCGCTCATACTTTGG+TGG 0.502666 1:+59261769 None:intergenic
AGACAAAATCTTAAGTATCA+AGG 0.503794 1:+59260802 None:intergenic
GAATGTCAGCGAACTTGGAC+TGG 0.509662 1:-59261111 MsG0180003258.01.T01:CDS
TCACGATCCAAAAGATAAGC+AGG 0.510830 1:+59262663 None:intergenic
CCCCACACAACTTTCCCTAC+AGG 0.513598 1:+59261083 None:intergenic
CTTGGACTGGTTACACCTGT+AGG 0.513953 1:-59261098 MsG0180003258.01.T01:CDS
TACCTCAATTATAGATCCCC+TGG 0.514336 1:+59261615 None:intergenic
GAGTACCTTGTGTTAGGCGG+TGG 0.521773 1:+59264185 None:intergenic
GACACCAAGAGAGGTGTACA+TGG 0.523657 1:+59261642 None:intergenic
TGCGGGTTTCGTGATCGAAA+CGG 0.524765 1:+59263207 None:intergenic
CTATTGGTCTTAAGGGAAAG+AGG 0.536215 1:-59263012 MsG0180003258.01.T01:CDS
TGTGGAGAAGCTACTCTCGT+TGG 0.537395 1:+59264234 None:intergenic
CGACTGACATACCTTTGCTC+TGG 0.539758 1:+59262632 None:intergenic
TTTAAGGTCAGATCAAGCAT+TGG 0.541841 1:-59260909 MsG0180003258.01.T01:exon
CACCTGTAGGGAAAGTTGTG+TGG 0.542031 1:-59261085 MsG0180003258.01.T01:CDS
ACCTGTAGGGAAAGTTGTGT+GGG 0.546540 1:-59261084 MsG0180003258.01.T01:CDS
AACCCGCAAGCGCAAGAGAG+AGG 0.557787 1:-59263192 MsG0180003258.01.T01:CDS
ATAACGGGACTCTTTCGCCT+CGG 0.563585 1:+59263471 None:intergenic
GCTACTCTCGTTGGAGCCTG+GGG 0.565299 1:+59264243 None:intergenic
ATTTCCTCAGGTACGTCCTG+TGG 0.566309 1:-59262317 MsG0180003258.01.T01:intron
GCGCAAGAGAGAGGCTCGTG+AGG 0.578621 1:-59263183 MsG0180003258.01.T01:CDS
GGGGATTCAACTATTGGAGG+CGG 0.578835 1:+59264263 None:intergenic
AAGGTCAGATCAAGCATTGG+AGG 0.579837 1:-59260906 MsG0180003258.01.T01:three_prime_UTR
ATGTTCAAAGTGGTCCGCAC+CGG 0.580559 1:-59262283 MsG0180003258.01.T01:CDS
TTCCAGTGGTATTGAAGGTG+TGG 0.581269 1:-59263516 MsG0180003258.01.T01:intron
TTGGACTGGTTACACCTGTA+GGG 0.581528 1:-59261097 MsG0180003258.01.T01:CDS
TACCAGGGGATCTATAATTG+AGG 0.588901 1:-59261617 MsG0180003258.01.T01:intron
AGCTAAGCAATGGAAGAGAA+TGG 0.593298 1:-59261833 MsG0180003258.01.T01:CDS
GAATGCAGGTAAATATGCCC+AGG 0.594561 1:-59260980 MsG0180003258.01.T01:intron
ACCAACAACCCAGAAAATGA+TGG 0.595399 1:-59260955 MsG0180003258.01.T01:CDS
CTCTCTTGGTGTCATTACCA+GGG 0.600053 1:-59261632 MsG0180003258.01.T01:CDS
CATCGAATGTTTGACAGTGG+CGG 0.605930 1:+59260836 None:intergenic
GACTGCCACCGCCTAACACA+AGG 0.612063 1:-59264190 MsG0180003258.01.T01:intron
GTTAGGCGGTGGCAGTCTGG+CGG 0.613891 1:+59264196 None:intergenic
TTTCATCGAATGTTTGACAG+TGG 0.618780 1:+59260833 None:intergenic
TCTCTTGGTGTCATTACCAG+GGG 0.619690 1:-59261631 MsG0180003258.01.T01:CDS
TGCATGAAGAGTCAACATCT+AGG 0.620336 1:-59262722 MsG0180003258.01.T01:CDS
ATTGAGTACCTTGTGTTAGG+CGG 0.629236 1:+59264182 None:intergenic
ACACCAAGAGAGGTGTACAT+GGG 0.632419 1:+59261643 None:intergenic
TTTAGTGAAACGCAATCCAG+TGG 0.635182 1:+59261741 None:intergenic
TGAAAACACGACCAGAGCAA+AGG 0.642977 1:-59262643 MsG0180003258.01.T01:intron
TTGCACTTGAAGGTAACTAG+TGG 0.649988 1:-59260679 MsG0180003258.01.T01:three_prime_UTR
AGAGTCAACATCTAGGCGCA+AGG 0.652247 1:-59262715 MsG0180003258.01.T01:CDS
GCTGATGATAATGTTCAGGA+AGG 0.654350 1:-59262693 MsG0180003258.01.T01:CDS
CCTGGTAATGACACCAAGAG+AGG 0.664047 1:+59261633 None:intergenic
CAACACATGCTATAAAGCCG+AGG 0.665525 1:-59263488 MsG0180003258.01.T01:five_prime_UTR
TTAGTGAAACGCAATCCAGT+GGG 0.670436 1:+59261742 None:intergenic
CTACTCTCGTTGGAGCCTGG+GGG 0.671441 1:+59264244 None:intergenic
CGGTCTATATGATCTCCTTG+AGG 0.680493 1:+59263251 None:intergenic
CACCAAGAGAGGTGTACATG+GGG 0.684428 1:+59261644 None:intergenic
GGGTTTCGTGATCGAAACGG+CGG 0.704227 1:+59263210 None:intergenic
TGAAGATGAAATGTTCAAAG+TGG 0.707953 1:-59262293 MsG0180003258.01.T01:CDS
CCTGTAGGGAAAGTTGTGTG+GGG 0.733461 1:-59261083 MsG0180003258.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGTTTATTTCTTAATTAT+AGG - Chr1:59262856-59262875 MsG0180003258.01.T01:intron 10.0%
!! ATACTATTGTTCTATTTATT+TGG - Chr1:59264199-59264218 MsG0180003258.01.T01:five_prime_UTR 15.0%
!! CATAAAATGTTAAAAGTAAT+TGG + Chr1:59261839-59261858 None:intergenic 15.0%
!! TATTCTATAAGCAATTAATA+TGG - Chr1:59263677-59263696 MsG0180003258.01.T01:intron 15.0%
!!! AAATCTAAAATCAAATTTTG+TGG - Chr1:59260874-59260893 MsG0180003258.01.T01:three_prime_UTR 15.0%
!!! AATTTTGTGGTTTTATAATT+TGG - Chr1:59260887-59260906 MsG0180003258.01.T01:three_prime_UTR 15.0%
!!! ATTTTGTGGTTTTATAATTT+GGG - Chr1:59260888-59260907 MsG0180003258.01.T01:three_prime_UTR 15.0%
!!! TAAGAAATAAACATATTTTC+TGG + Chr1:59262851-59262870 None:intergenic 15.0%
!! AAAACAACTTAGAACTTATA+TGG - Chr1:59263583-59263602 MsG0180003258.01.T01:intron 20.0%
!! AAAACTAGTTCAGTTTATAA+CGG + Chr1:59261475-59261494 None:intergenic 20.0%
!! ACATTCAATCTTGAAATTAT+TGG - Chr1:59263239-59263258 MsG0180003258.01.T01:CDS 20.0%
!! ATATTGTCATAAATCTAACT+GGG - Chr1:59261866-59261885 MsG0180003258.01.T01:intron 20.0%
!! ATTCGATAAATTTGAAGATT+AGG + Chr1:59260811-59260830 None:intergenic 20.0%
!! TATAAATCTGTTAGATTCAT+TGG + Chr1:59264274-59264293 None:intergenic 20.0%
!! TATATTGTCATAAATCTAAC+TGG - Chr1:59261865-59261884 MsG0180003258.01.T01:intron 20.0%
!! TATTGTTAGTATCAAATTCA+TGG - Chr1:59262747-59262766 MsG0180003258.01.T01:intron 20.0%
!! TCAAATTATCAAATTATGAC+CGG - Chr1:59261166-59261185 MsG0180003258.01.T01:intron 20.0%
!! TGTTATATTTCATGTGTTTA+TGG - Chr1:59262022-59262041 MsG0180003258.01.T01:intron 20.0%
!!! TAAGTAAGTTTTGATAGATA+TGG - Chr1:59262123-59262142 MsG0180003258.01.T01:intron 20.0%
!!! TTAAAATTACATTTTTCCAG+TGG - Chr1:59261397-59261416 MsG0180003258.01.T01:intron 20.0%
! AAACTAGTTCAGTTTATAAC+GGG + Chr1:59261474-59261493 None:intergenic 25.0%
! AAGAAAAAACAAATACAGAG+TGG + Chr1:59262512-59262531 None:intergenic 25.0%
! AAGAGTAAGTATTACATCTA+TGG - Chr1:59262674-59262693 MsG0180003258.01.T01:CDS 25.0%
! AATACATTGTAAGCACATTA+CGG - Chr1:59263002-59263021 MsG0180003258.01.T01:CDS 25.0%
! ACATAAACATCATCAAATGA+AGG + Chr1:59261562-59261581 None:intergenic 25.0%
! ACGAAACCAAAATCAAAATA+GGG + Chr1:59261632-59261651 None:intergenic 25.0%
! ACGTTATTATAACTCAAGAA+AGG + Chr1:59262574-59262593 None:intergenic 25.0%
! ACTAAGCTGTATAAACTATA+AGG + Chr1:59260969-59260988 None:intergenic 25.0%
! AGAAAAAACAAATACAGAGT+GGG + Chr1:59262511-59262530 None:intergenic 25.0%
! AGACAAAATCTTAAGTATCA+AGG + Chr1:59264128-59264147 None:intergenic 25.0%
! ATAAACATCATCAAATGAAG+GGG + Chr1:59261560-59261579 None:intergenic 25.0%
! ATTATTTGGCACTTATATCT+TGG - Chr1:59263871-59263890 MsG0180003258.01.T01:intron 25.0%
! CAAAAAGTAATCAAATGTGT+TGG + Chr1:59261133-59261152 None:intergenic 25.0%
! CATAAACATCATCAAATGAA+GGG + Chr1:59261561-59261580 None:intergenic 25.0%
! CTAAGCTGTATAAACTATAA+GGG + Chr1:59260968-59260987 None:intergenic 25.0%
! GAATCATTATCAAAATGTGT+TGG - Chr1:59263764-59263783 MsG0180003258.01.T01:intron 25.0%
! TTACAAAAGCAACATTTGTT+GGG - Chr1:59263117-59263136 MsG0180003258.01.T01:intron 25.0%
! TTTAACTGATATTGATGCAT+TGG - Chr1:59263914-59263933 MsG0180003258.01.T01:intron 25.0%
! TTTGATAGATATGGATTTGT+CGG - Chr1:59262132-59262151 MsG0180003258.01.T01:intron 25.0%
!! AATGGCAAAGAATTTTTGTT+TGG - Chr1:59264152-59264171 MsG0180003258.01.T01:intron 25.0%
!! ATACTTACTCTTTCTTTTAC+CGG + Chr1:59262666-59262685 None:intergenic 25.0%
!! TTAAGATTTTGTCTGGAAAA+TGG - Chr1:59264134-59264153 MsG0180003258.01.T01:intron 25.0%
!! TTGATACTTAAGATTTTGTC+TGG - Chr1:59264127-59264146 MsG0180003258.01.T01:intron 25.0%
!! TTGGATTTTTATATGAATGC+AGG - Chr1:59263933-59263952 MsG0180003258.01.T01:intron 25.0%
!! TTTTGATGTTATCATATGAC+GGG - Chr1:59262938-59262957 MsG0180003258.01.T01:intron 25.0%
!!! AAAGAATTTTTGTTTGGATC+CGG - Chr1:59264158-59264177 MsG0180003258.01.T01:intron 25.0%
!!! AAATATAGCTTTAGTCTGTT+TGG - Chr1:59263451-59263470 MsG0180003258.01.T01:five_prime_UTR 25.0%
!!! AACTAGTTTTGTGTTTTTAG+TGG - Chr1:59261485-59261504 MsG0180003258.01.T01:intron 25.0%
!!! AATGCTGTTTTACTTGTTTA+AGG - Chr1:59264002-59264021 MsG0180003258.01.T01:intron 25.0%
!!! CTTATTTTTGAGCTTATGAA+AGG - Chr1:59260924-59260943 MsG0180003258.01.T01:exon 25.0%
!!! GTTTTGATGTTATCATATGA+CGG - Chr1:59262937-59262956 MsG0180003258.01.T01:intron 25.0%
!!! TTACATCCCTATTTTGATTT+TGG - Chr1:59261623-59261642 MsG0180003258.01.T01:CDS 25.0%
!!! TTTGACAGTTTCACATTTTT+TGG - Chr1:59261314-59261333 MsG0180003258.01.T01:intron 25.0%
!!! TTTTTCGGCATAATTTTTCT+TGG + Chr1:59261945-59261964 None:intergenic 25.0%
AAAACACAATGACTTAGAGT+TGG + Chr1:59262922-59262941 None:intergenic 30.0%
AAAATTGAGTACCTTGTGTT+AGG + Chr1:59260751-59260770 None:intergenic 30.0%
AAGTACGTGAGTAAATGAAA+AGG + Chr1:59263722-59263741 None:intergenic 30.0%
ACAGTAACAGAAAATTCTTG+AGG + Chr1:59261595-59261614 None:intergenic 30.0%
AGTACGTGAGTAAATGAAAA+GGG + Chr1:59263721-59263740 None:intergenic 30.0%
ATACATTGTAAGCACATTAC+GGG - Chr1:59263003-59263022 MsG0180003258.01.T01:CDS 30.0%
ATATCTTGGTTTCATCATCA+TGG - Chr1:59263885-59263904 MsG0180003258.01.T01:intron 30.0%
ATCAATATGCATGCAATCTA+AGG + Chr1:59263030-59263049 None:intergenic 30.0%
ATGTGATGACATGATTGAAA+TGG - Chr1:59262722-59262741 MsG0180003258.01.T01:CDS 30.0%
ATTTCAATCATGTCATCACA+TGG + Chr1:59262723-59262742 None:intergenic 30.0%
ATTTCATGCACTTCTAATTG+AGG - Chr1:59263340-59263359 MsG0180003258.01.T01:intron 30.0%
CAAAAATAAGCCAATCCAAA+CGG + Chr1:59260914-59260933 None:intergenic 30.0%
CTACATCCATAAACAAAAAG+GGG + Chr1:59262828-59262847 None:intergenic 30.0%
GACGAAACCAAAATCAAAAT+AGG + Chr1:59261633-59261652 None:intergenic 30.0%
GCTACATCCATAAACAAAAA+GGG + Chr1:59262829-59262848 None:intergenic 30.0%
GTTACAAAAGCAACATTTGT+TGG - Chr1:59263116-59263135 MsG0180003258.01.T01:intron 30.0%
GTTTATACAGCTTAGTGAAT+AGG - Chr1:59260973-59260992 MsG0180003258.01.T01:CDS 30.0%
TAGAATTCGAATAACAAGAG+AGG + Chr1:59260855-59260874 None:intergenic 30.0%
TGAAGATGAAATGTTCAAAG+TGG - Chr1:59262634-59262653 MsG0180003258.01.T01:intron 30.0%
TGTGATGACATGATTGAAAT+GGG - Chr1:59262723-59262742 MsG0180003258.01.T01:CDS 30.0%
TTAGTGTATTCAAGAGTTGT+TGG - Chr1:59262086-59262105 MsG0180003258.01.T01:intron 30.0%
TTATTACTTCTTGCACTTGA+AGG - Chr1:59264238-59264257 MsG0180003258.01.T01:five_prime_UTR 30.0%
TTGATAGAAGAAGAGGAAAA+GGG + Chr1:59261805-59261824 None:intergenic 30.0%
TTTGATAGAAGAAGAGGAAA+AGG + Chr1:59261806-59261825 None:intergenic 30.0%
! AAAGTGTTTGATAGAAGAAG+AGG + Chr1:59261812-59261831 None:intergenic 30.0%
! ATGTGTATTTTCATTTCCTC+AGG - Chr1:59262598-59262617 MsG0180003258.01.T01:intron 30.0%
! GGCTTCTAGTATTATGATTT+TGG - Chr1:59262695-59262714 MsG0180003258.01.T01:CDS 30.0%
! TTATGCAACCATCATTTTCT+GGG + Chr1:59263983-59264002 None:intergenic 30.0%
! TTGAATGTGCATTTAAGTTC+AGG + Chr1:59263227-59263246 None:intergenic 30.0%
! TTTATGCAACCATCATTTTC+TGG + Chr1:59263984-59264003 None:intergenic 30.0%
! TTTTCCGTTCTCACAATTTA+AGG - Chr1:59263370-59263389 MsG0180003258.01.T01:intron 30.0%
!! AAACAGACTATTGGTCTTAA+GGG - Chr1:59261908-59261927 MsG0180003258.01.T01:intron 30.0%
!! AAATGTGTTGGTCATTTTCT+TGG - Chr1:59263776-59263795 MsG0180003258.01.T01:intron 30.0%
!! GTTGTTTTCATAATCTATCC+TGG + Chr1:59263571-59263590 None:intergenic 30.0%
!! TCATTTTCAAGAGACAACAA+TGG - Chr1:59261519-59261538 MsG0180003258.01.T01:intron 30.0%
!! TTTGTATCATTTTAGCCTCA+AGG - Chr1:59261661-59261680 MsG0180003258.01.T01:CDS 30.0%
!!! TTGTGTTTTTAGTGGTTACT+TGG - Chr1:59261493-59261512 MsG0180003258.01.T01:intron 30.0%
AACAAATACAGAGTGGGAAT+TGG + Chr1:59262505-59262524 None:intergenic 35.0%
ACACCATTAAGCAAAAAGGA+AGG - Chr1:59262411-59262430 MsG0180003258.01.T01:intron 35.0%
ACAGCTTAGTGAATAGGATT+AGG - Chr1:59260979-59260998 MsG0180003258.01.T01:intron 35.0%
ACTACAAAAATCATGTGAGC+AGG + Chr1:59264053-59264072 None:intergenic 35.0%
AGCAACACCATTAAGCAAAA+AGG - Chr1:59262407-59262426 MsG0180003258.01.T01:intron 35.0%
AGTGATTGAAAAGGTCATGT+GGG + Chr1:59263409-59263428 None:intergenic 35.0%
ATCCATAAACAAAAAGGGGA+GGG + Chr1:59262824-59262843 None:intergenic 35.0%
ATTCGAGTGAAACAGACTAT+TGG - Chr1:59261899-59261918 MsG0180003258.01.T01:intron 35.0%
CAGATTAGACATCTCTAACT+TGG + Chr1:59261253-59261272 None:intergenic 35.0%
CTTTAGCAAAGAAAGAAGCA+GGG - Chr1:59262529-59262548 MsG0180003258.01.T01:intron 35.0%
GAAGCAGATCTGATTGTATA+TGG - Chr1:59263058-59263077 MsG0180003258.01.T01:intron 35.0%
GACCGTCACATAAAAGAAAA+TGG - Chr1:59261694-59261713 MsG0180003258.01.T01:CDS 35.0%
GATGCCTTAAATTGTGAGAA+CGG + Chr1:59263377-59263396 None:intergenic 35.0%
GGCTACATCCATAAACAAAA+AGG + Chr1:59262830-59262849 None:intergenic 35.0%
GTACATGTCAGTGATTGAAA+AGG + Chr1:59263418-59263437 None:intergenic 35.0%
TAAATGAAAAGGGTTCTGTG+TGG + Chr1:59263711-59263730 None:intergenic 35.0%
TAACTTGGCAACATATAGAG+AGG + Chr1:59261238-59261257 None:intergenic 35.0%
TACATTGTAAGCACATTACG+GGG - Chr1:59263004-59263023 MsG0180003258.01.T01:CDS 35.0%
TCTTTAGCAAAGAAAGAAGC+AGG - Chr1:59262528-59262547 MsG0180003258.01.T01:intron 35.0%
TTAAGCAAAAAGGAAGGAGA+AGG - Chr1:59262417-59262436 MsG0180003258.01.T01:intron 35.0%
TTCTTGGTTACTGTGATTTC+AGG - Chr1:59263792-59263811 MsG0180003258.01.T01:intron 35.0%
TTGTGTGGGGTAAGATTATT+TGG - Chr1:59263857-59263876 MsG0180003258.01.T01:intron 35.0%
TTTCATCGAATGTTTGACAG+TGG + Chr1:59264097-59264116 None:intergenic 35.0%
! ATCATTTTGCAGCTAAGCAA+TGG - Chr1:59263084-59263103 MsG0180003258.01.T01:intron 35.0%
! CATGATTTTTGTAGTCTCGT+AGG - Chr1:59264058-59264077 MsG0180003258.01.T01:intron 35.0%
! GTTTTATAATTTGGGCCGTT+TGG - Chr1:59260896-59260915 MsG0180003258.01.T01:three_prime_UTR 35.0%
! TCTCCTTCCTTTTTGCTTAA+TGG + Chr1:59262417-59262436 None:intergenic 35.0%
! TTTAAGGTCAGATCAAGCAT+TGG - Chr1:59264018-59264037 MsG0180003258.01.T01:intron 35.0%
!! CATTTTTCCAGTGGTATTGA+AGG - Chr1:59261406-59261425 MsG0180003258.01.T01:intron 35.0%
!! CCTTTTCATCTGTGCTTTTT+CGG + Chr1:59261960-59261979 None:intergenic 35.0%
!! GAAACAGACTATTGGTCTTA+AGG - Chr1:59261907-59261926 MsG0180003258.01.T01:intron 35.0%
!! GGCCATTTTCTTTTATGTGA+CGG + Chr1:59261699-59261718 None:intergenic 35.0%
!!! GACAGTTTCACATTTTTTGG+TGG - Chr1:59261317-59261336 MsG0180003258.01.T01:intron 35.0%
AACATTTCATCTTCAGCCAC+AGG + Chr1:59262629-59262648 None:intergenic 40.0%
AACTGTCTTCCAAAACAGCA+TGG + Chr1:59262323-59262342 None:intergenic 40.0%
AAGCAGGGAAAGACATGAAA+CGG - Chr1:59262544-59262563 MsG0180003258.01.T01:intron 40.0%
ACCAACAACCCAGAAAATGA+TGG - Chr1:59263972-59263991 MsG0180003258.01.T01:intron 40.0%
AGCTAAGCAATGGAAGAGAA+TGG - Chr1:59263094-59263113 MsG0180003258.01.T01:intron 40.0%
AGGAAAAGGGTTAAACCTTC+TGG + Chr1:59261792-59261811 None:intergenic 40.0%
ATAATTTGGGCCGTTTGGAT+TGG - Chr1:59260901-59260920 MsG0180003258.01.T01:three_prime_UTR 40.0%
ATGAATCGCTCATACTTTGG+TGG + Chr1:59263161-59263180 None:intergenic 40.0%
ATTGAGTACCTTGTGTTAGG+CGG + Chr1:59260748-59260767 None:intergenic 40.0%
CAGTGATTGAAAAGGTCATG+TGG + Chr1:59263410-59263429 None:intergenic 40.0%
CATCCATAAACAAAAAGGGG+AGG + Chr1:59262825-59262844 None:intergenic 40.0%
CGAATGAATCGCTCATACTT+TGG + Chr1:59263164-59263183 None:intergenic 40.0%
GGAAAAGGGTTAAACCTTCT+GGG + Chr1:59261791-59261810 None:intergenic 40.0%
GGAGTGAAGAAAAACCCTAA+TGG - Chr1:59263260-59263279 MsG0180003258.01.T01:intron 40.0%
TACCAGGGGATCTATAATTG+AGG - Chr1:59263310-59263329 MsG0180003258.01.T01:intron 40.0%
TACCTCAATTATAGATCCCC+TGG + Chr1:59263315-59263334 None:intergenic 40.0%
TACTACAACATGACAGTGTC+CGG + Chr1:59264180-59264199 None:intergenic 40.0%
TCACGATCCAAAAGATAAGC+AGG + Chr1:59262267-59262286 None:intergenic 40.0%
TCCATAAACAAAAAGGGGAG+GGG + Chr1:59262823-59262842 None:intergenic 40.0%
TGAATGTGCAATCTCACCTT+AGG + Chr1:59262466-59262485 None:intergenic 40.0%
TGCATGAAGAGTCAACATCT+AGG - Chr1:59262205-59262224 MsG0180003258.01.T01:intron 40.0%
TTAGTGAAACGCAATCCAGT+GGG + Chr1:59263188-59263207 None:intergenic 40.0%
TTGATTCTCTTGCACACTCT+AGG - Chr1:59262350-59262369 MsG0180003258.01.T01:intron 40.0%
TTGCACTTGAAGGTAACTAG+TGG - Chr1:59264248-59264267 MsG0180003258.01.T01:five_prime_UTR 40.0%
TTTAGTGAAACGCAATCCAG+TGG + Chr1:59263189-59263208 None:intergenic 40.0%
! ACCATCATTTTCTGGGTTGT+TGG + Chr1:59263976-59263995 None:intergenic 40.0%
! ACGCCATCTATCAAAAGTGT+CGG + Chr1:59261079-59261098 None:intergenic 40.0%
! AGCTGTTCCTGCTTATCTTT+TGG - Chr1:59262257-59262276 MsG0180003258.01.T01:intron 40.0%
! CAAGGCTGATGATAATGTTC+AGG - Chr1:59262230-59262249 MsG0180003258.01.T01:intron 40.0%
! CCGAAAAAGCACAGATGAAA+AGG - Chr1:59261957-59261976 MsG0180003258.01.T01:intron 40.0%
! GATGCTGCAATTTTTGTGTG+CGG - Chr1:59262883-59262902 MsG0180003258.01.T01:intron 40.0%
! GCTGATGATAATGTTCAGGA+AGG - Chr1:59262234-59262253 MsG0180003258.01.T01:intron 40.0%
! GGTTCACAACCACTCTAAAA+AGG - Chr1:59261765-59261784 MsG0180003258.01.T01:CDS 40.0%
! TACTCTTTCTTTTACCGGTG+CGG + Chr1:59262661-59262680 None:intergenic 40.0%
!! AAGCACTGACATTACTGACA+CGG + Chr1:59261203-59261222 None:intergenic 40.0%
!! CTATTGGTCTTAAGGGAAAG+AGG - Chr1:59261915-59261934 MsG0180003258.01.T01:intron 40.0%
!! CTCATACTTTGGTGGTTTTC+TGG + Chr1:59263153-59263172 None:intergenic 40.0%
ACAATGTTCATACGCTCTCC+AGG - Chr1:59263550-59263569 MsG0180003258.01.T01:intron 45.0%
ACCTGTAGGGAAAGTTGTGT+GGG - Chr1:59263843-59263862 MsG0180003258.01.T01:intron 45.0%
AGCAGGGAAAGACATGAAAC+GGG - Chr1:59262545-59262564 MsG0180003258.01.T01:intron 45.0%
ATGGGATGTTCCTCTACCTA+AGG - Chr1:59262447-59262466 MsG0180003258.01.T01:intron 45.0%
CAAAAAGGAAGGAGAAGGCT+GGG - Chr1:59262422-59262441 MsG0180003258.01.T01:intron 45.0%
CAACACATGCTATAAAGCCG+AGG - Chr1:59261439-59261458 MsG0180003258.01.T01:intron 45.0%
CATCGAATGTTTGACAGTGG+CGG + Chr1:59264094-59264113 None:intergenic 45.0%
CGGTCTATATGATCTCCTTG+AGG + Chr1:59261679-59261698 None:intergenic 45.0%
GAATGCAGGTAAATATGCCC+AGG - Chr1:59263947-59263966 MsG0180003258.01.T01:intron 45.0%
GACACTGACACTAACAGACA+CGG + Chr1:59261105-59261124 None:intergenic 45.0%
GCTCTTGTCTGTCAACAGTT+TGG - Chr1:59262179-59262198 MsG0180003258.01.T01:intron 45.0%
GGAGGGGACAAAGAATGATA+AGG + Chr1:59262807-59262826 None:intergenic 45.0%
GTGTCTGTTAGTGTCAGTGT+CGG - Chr1:59261104-59261123 MsG0180003258.01.T01:CDS 45.0%
TGAAAACACGACCAGAGCAA+AGG - Chr1:59262284-59262303 MsG0180003258.01.T01:CDS 45.0%
TGCAATCTCACCTTAGGTAG+AGG + Chr1:59262460-59262479 None:intergenic 45.0%
! AAAGCAACATTTGTTGGGCC+TGG - Chr1:59263122-59263141 MsG0180003258.01.T01:intron 45.0%
! ACACCAAGAGAGGTGTACAT+GGG + Chr1:59263287-59263306 None:intergenic 45.0%
! CGAATAACAAGAGAGGTGTG+AGG + Chr1:59260848-59260867 None:intergenic 45.0%
! TTCCAGTGGTATTGAAGGTG+TGG - Chr1:59261411-59261430 MsG0180003258.01.T01:intron 45.0%
!! AAGGTCAGATCAAGCATTGG+AGG - Chr1:59264021-59264040 MsG0180003258.01.T01:intron 45.0%
!! CTCTCTTGGTGTCATTACCA+GGG - Chr1:59263295-59263314 MsG0180003258.01.T01:intron 45.0%
!! GCACCGACACTTTTGATAGA+TGG - Chr1:59261073-59261092 MsG0180003258.01.T01:intron 45.0%
!! GGTGGTTTTCTGGTAAAACC+AGG + Chr1:59263143-59263162 None:intergenic 45.0%
!! TCCCCTCCCCTTTTTGTTTA+TGG - Chr1:59262819-59262838 MsG0180003258.01.T01:intron 45.0%
!! TCTCTTGGTGTCATTACCAG+GGG - Chr1:59263296-59263315 MsG0180003258.01.T01:intron 45.0%
!! TTCTGGGTTGTTGGTAACCT+GGG + Chr1:59263967-59263986 None:intergenic 45.0%
!! TTGGACTGGTTACACCTGTA+GGG - Chr1:59263830-59263849 MsG0180003258.01.T01:intron 45.0%
!! TTTCTGGGTTGTTGGTAACC+TGG + Chr1:59263968-59263987 None:intergenic 45.0%
!!! CGGAGTCACAACTAGTTTTG+TGG + Chr1:59260714-59260733 None:intergenic 45.0%
AGAGTCAACATCTAGGCGCA+AGG - Chr1:59262212-59262231 MsG0180003258.01.T01:intron 50.0%
AGCCTGGGGGATTCAACTAT+TGG + Chr1:59260673-59260692 None:intergenic 50.0%
AGGTGTACATGGGGCCATTA+GGG + Chr1:59263277-59263296 None:intergenic 50.0%
ATGTTCAAAGTGGTCCGCAC+CGG - Chr1:59262644-59262663 MsG0180003258.01.T01:CDS 50.0%
ATTTCCTCAGGTACGTCCTG+TGG - Chr1:59262610-59262629 MsG0180003258.01.T01:intron 50.0%
CAACTATTGGAGGCGGCTTA+GGG + Chr1:59260660-59260679 None:intergenic 50.0%
CACCACACCTTCAATACCAC+TGG + Chr1:59261416-59261435 None:intergenic 50.0%
CACCTGTAGGGAAAGTTGTG+TGG - Chr1:59263842-59263861 MsG0180003258.01.T01:intron 50.0%
CAGGTGAATGTCAGCGAACT+TGG - Chr1:59263811-59263830 MsG0180003258.01.T01:intron 50.0%
CCTGTAGGGAAAGTTGTGTG+GGG - Chr1:59263844-59263863 MsG0180003258.01.T01:intron 50.0%
CGACTGACATACCTTTGCTC+TGG + Chr1:59262298-59262317 None:intergenic 50.0%
CTCCAATAGTTGAATCCCCC+AGG - Chr1:59260668-59260687 MsG0180003258.01.T01:three_prime_UTR 50.0%
CTGGGGGATTCAACTATTGG+AGG + Chr1:59260670-59260689 None:intergenic 50.0%
GAAAGGAACACCGTCTGAGA+CGG - Chr1:59260941-59260960 MsG0180003258.01.T01:CDS 50.0%
GAAGGAGAAGGCTGGGAAAT+GGG - Chr1:59262429-59262448 MsG0180003258.01.T01:intron 50.0%
GAATGTCAGCGAACTTGGAC+TGG - Chr1:59263816-59263835 MsG0180003258.01.T01:intron 50.0%
GCAAAAAGGAAGGAGAAGGC+TGG - Chr1:59262421-59262440 MsG0180003258.01.T01:intron 50.0%
GCAGGGAAAGACATGAAACG+GGG - Chr1:59262546-59262565 MsG0180003258.01.T01:intron 50.0%
GGGGATTCAACTATTGGAGG+CGG + Chr1:59260667-59260686 None:intergenic 50.0%
TCAACTATTGGAGGCGGCTT+AGG + Chr1:59260661-59260680 None:intergenic 50.0%
TGACACGGACACATCAACAC+CGG + Chr1:59261188-59261207 None:intergenic 50.0%
TGCGGGTTTCGTGATCGAAA+CGG + Chr1:59261723-59261742 None:intergenic 50.0%
TGTGGAGAAGCTACTCTCGT+TGG + Chr1:59260696-59260715 None:intergenic 50.0%
! AAAGGAACACCGTCTGAGAC+GGG - Chr1:59260942-59260961 MsG0180003258.01.T01:CDS 50.0%
! ATAACGGGACTCTTTCGCCT+CGG + Chr1:59261459-59261478 None:intergenic 50.0%
! CACCAAGAGAGGTGTACATG+GGG + Chr1:59263286-59263305 None:intergenic 50.0%
! CCACTCTAAAAAGGCCCAGA+AGG - Chr1:59261774-59261793 MsG0180003258.01.T01:CDS 50.0%
! CCTGGTAATGACACCAAGAG+AGG + Chr1:59263297-59263316 None:intergenic 50.0%
! GACACCAAGAGAGGTGTACA+TGG + Chr1:59263288-59263307 None:intergenic 50.0%
!! CCTCTCTTGGTGTCATTACC+AGG - Chr1:59263294-59263313 MsG0180003258.01.T01:intron 50.0%
!! CCTTCTGGGCCTTTTTAGAG+TGG + Chr1:59261777-59261796 None:intergenic 50.0%
!! CTTGGACTGGTTACACCTGT+AGG - Chr1:59263829-59263848 MsG0180003258.01.T01:intron 50.0%
!! TCAGTCGTGCCATGCTGTTT+TGG - Chr1:59262311-59262330 MsG0180003258.01.T01:intron 50.0%
AAGCTACTCTCGTTGGAGCC+TGG + Chr1:59260689-59260708 None:intergenic 55.0%
AGCTACTCTCGTTGGAGCCT+GGG + Chr1:59260688-59260707 None:intergenic 55.0%
CCCCACACAACTTTCCCTAC+AGG + Chr1:59263847-59263866 None:intergenic 55.0%
CTATAAGGGCCCGTCTCAGA+CGG + Chr1:59260954-59260973 None:intergenic 55.0%
GAGCGATTCATTCGTCCCAC+TGG - Chr1:59263170-59263189 MsG0180003258.01.T01:CDS 55.0%
GAGGTGTACATGGGGCCATT+AGG + Chr1:59263278-59263297 None:intergenic 55.0%
GAGTACCTTGTGTTAGGCGG+TGG + Chr1:59260745-59260764 None:intergenic 55.0%
GGAAGGAGAAGGCTGGGAAA+TGG - Chr1:59262428-59262447 MsG0180003258.01.T01:intron 55.0%
GGGTTTCGTGATCGAAACGG+CGG + Chr1:59261720-59261739 None:intergenic 55.0%
AACCCGCAAGCGCAAGAGAG+AGG - Chr1:59261735-59261754 MsG0180003258.01.T01:CDS 60.0%
AGCCTCTCTCTTGCGCTTGC+GGG + Chr1:59261740-59261759 None:intergenic 60.0%
CTACTCTCGTTGGAGCCTGG+GGG + Chr1:59260686-59260705 None:intergenic 60.0%
GACTGCCACCGCCTAACACA+AGG - Chr1:59260737-59260756 MsG0180003258.01.T01:three_prime_UTR 60.0%
GAGCCTCTCTCTTGCGCTTG+CGG + Chr1:59261741-59261760 None:intergenic 60.0%
GCTACTCTCGTTGGAGCCTG+GGG + Chr1:59260687-59260706 None:intergenic 60.0%
GGCCCCATGTACACCTCTCT+TGG - Chr1:59263281-59263300 MsG0180003258.01.T01:intron 60.0%
TCAGCCACAGGACGTACCTG+AGG + Chr1:59262617-59262636 None:intergenic 60.0%
TGTGTTAGGCGGTGGCAGTC+TGG + Chr1:59260737-59260756 None:intergenic 60.0%
GCGCAAGAGAGAGGCTCGTG+AGG - Chr1:59261744-59261763 MsG0180003258.01.T01:CDS 65.0%
GTTAGGCGGTGGCAGTCTGG+CGG + Chr1:59260734-59260753 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 59260653 59264296 59260653 ID=MsG0180003258.01;Name=MsG0180003258.01
Chr1 mRNA 59260653 59264296 59260653 ID=MsG0180003258.01.T01;Parent=MsG0180003258.01;Name=MsG0180003258.01.T01;_AED=0.43;_eAED=0.43;_QI=227|0.87|1|1|0.5|0.44|9|274|237
Chr1 exon 59260653 59260994 59260653 ID=MsG0180003258.01.T01:exon:49121;Parent=MsG0180003258.01.T01
Chr1 exon 59261084 59261135 59261084 ID=MsG0180003258.01.T01:exon:49120;Parent=MsG0180003258.01.T01
Chr1 exon 59261618 59261853 59261618 ID=MsG0180003258.01.T01:exon:49119;Parent=MsG0180003258.01.T01
Chr1 exon 59262272 59262329 59262272 ID=MsG0180003258.01.T01:exon:49118;Parent=MsG0180003258.01.T01
Chr1 exon 59262644 59262752 59262644 ID=MsG0180003258.01.T01:exon:49117;Parent=MsG0180003258.01.T01
Chr1 exon 59262968 59263035 59262968 ID=MsG0180003258.01.T01:exon:49116;Parent=MsG0180003258.01.T01
Chr1 exon 59263154 59263273 59263154 ID=MsG0180003258.01.T01:exon:49115;Parent=MsG0180003258.01.T01
Chr1 exon 59263409 59263532 59263409 ID=MsG0180003258.01.T01:exon:49114;Parent=MsG0180003258.01.T01
Chr1 exon 59264191 59264296 59264191 ID=MsG0180003258.01.T01:exon:49113;Parent=MsG0180003258.01.T01
Chr1 five_prime_UTR 59264191 59264296 59264191 ID=MsG0180003258.01.T01:five_prime_utr;Parent=MsG0180003258.01.T01
Chr1 five_prime_UTR 59263412 59263532 59263412 ID=MsG0180003258.01.T01:five_prime_utr;Parent=MsG0180003258.01.T01
Chr1 CDS 59263409 59263411 59263409 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59263154 59263273 59263154 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59262968 59263035 59262968 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59262644 59262752 59262644 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59262272 59262329 59262272 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59261618 59261853 59261618 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59261084 59261135 59261084 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 CDS 59260927 59260994 59260927 ID=MsG0180003258.01.T01:cds;Parent=MsG0180003258.01.T01
Chr1 three_prime_UTR 59260653 59260926 59260653 ID=MsG0180003258.01.T01:three_prime_utr;Parent=MsG0180003258.01.T01
Gene Sequence

>MsG0180003258.01.T01

ATGCCTCAAGGAGATCATATAGACCGTCACATAAAAGAAAATGGCCGCCGTTTCGATCACGAAACCCGCAAGCGCAAGAGAGAGGCTCGTGAGGTTCACAACCACTCTAAAAAGGCCCAGAAGACTATTGGTCTTAAGGGAAAGAGGAATGCCAAGAAAAATTATGCCGAAAAAGCACAGATGAAAAGGACTTTGGCGATGCATGAAGAGTCAACATCTAGGCGCAAGGCTGATGATAATGTTCAGGAAGGAGCTGTTCCTGCTTATCTTTTGGATCGTGAAAACACGACCAGAGCAAAGGTACGTCCTGTGGCTGAAGATGAAATGTTCAAAGTGGTCCGCACCGGTAAAAGAAAGACTAAGCAATGGAAGAGAATGGTTACAAAAGCAACATTTGTTGGGCCTGGTTTTACCAGAAAACCACCAAAGTATGAGCGATTCATTCGTCCCACTGGATTGCGTTTCACTAAAGCTCATGTCACTCATCCTGAACTTAAATGCACATTCAATCTTGAAATTATTGGAGTGAAGAAAAACCCTAATGGCCCCATGTACACCTCTCTTGGTGTCATTACCAGGGGATCTATAATTGAGGTGAATGTCAGCGAACTTGGACTGGTTACACCTGTAGGGAAAGTTGTGTGGGGTAAATATGCCCAGGTTACCAACAACCCAGAAAATGATGGTTGCATAAATGCTGTTTTACTTGTTTAA
Protein sequence

>MsG0180003258.01.T01

MPQGDHIDRHIKENGRRFDHETRKRKREAREVHNHSKKAQKTIGLKGKRNAKKNYAEKAQMKRTLAMHEESTSRRKADDNVQEGAVPAYLLDRENTTRAKVRPVAEDEMFKVVRTGKRKTKQWKRMVTKATFVGPGFTRKPPKYERFIRPTGLRFTKAHVTHPELKCTFNLEIIGVKKNPNGPMYTSLGVITRGSIIEVNVSELGLVTPVGKVVWGKYAQVTNNPENDGCINAVLLV*