AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000096.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000096.01.T01 MTR_1g023590 99.000 100 1 0 1 100 107 206 5.24e-66 197
MsG0180000096.01.T01 MTR_7g057770 90.000 100 10 0 1 100 107 206 1.34e-59 181
MsG0180000096.01.T01 MTR_5g083790 85.000 100 15 0 1 100 107 206 8.97e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000096.01.T01 AT4G13170 76.000 100 24 0 1 100 107 206 5.25e-51 159
MsG0180000096.01.T01 AT5G48760 76.000 100 24 0 1 100 107 206 1.84e-50 158
MsG0180000096.01.T01 AT5G48760 76.000 100 24 0 1 100 107 206 1.84e-50 158
MsG0180000096.01.T01 AT3G07110 77.000 100 23 0 1 100 107 206 2.98e-50 157
MsG0180000096.01.T01 AT3G07110 76.238 101 23 1 1 100 107 207 1.54e-48 153
MsG0180000096.01.T01 AT3G24830 73.000 100 27 0 1 100 107 206 3.90e-48 152

Find 43 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTATTATAAGGTTTAGTT+TGG 0.304616 1:-1386403 MsG0180000096.01.T01:three_prime_UTR
TTCCAGTAGTGTTGCCCCTT+TGG 0.316652 1:-1386244 MsG0180000096.01.T01:three_prime_UTR
GCTTGTTATTCTATGTTTGT+TGG 0.318515 1:-1386339 MsG0180000096.01.T01:three_prime_UTR
TATTTGTTAACATTATTATA+AGG 0.320139 1:-1386413 MsG0180000096.01.T01:three_prime_UTR
ACTATGAAGCTTATAGTTGT+TGG 0.329462 1:-1386182 MsG0180000096.01.T01:three_prime_UTR
CACCAAAGGGGCAACACTAC+TGG 0.369640 1:+1386242 None:intergenic
GGGAGCAAGGACTTCAAGTT+GGG 0.380650 1:+1386452 None:intergenic
TATAGGAGCTGGAGGATAAG+AGG 0.397991 1:-1386566 MsG0180000096.01.T01:intron
AATAACAAGCAAAACTGAAA+TGG 0.403835 1:+1386352 None:intergenic
ATATATGATGTTACACCAAA+GGG 0.406117 1:+1386229 None:intergenic
GAGGAAGGAACGCGCAGGTT+TGG 0.417217 1:-1386547 MsG0180000096.01.T01:CDS
TAGGGTGTTGAGACTTCAAA+AGG 0.434162 1:-1386744 MsG0180000096.01.T01:CDS
ATCCTCTTGATCTTGTCATA+TGG 0.437541 1:+1386883 None:intergenic
CGGGAGCAAGGACTTCAAGT+TGG 0.439216 1:+1386451 None:intergenic
AATAGTACTCAGTACTTAAC+GGG 0.449959 1:+1386432 None:intergenic
AAATAGTACTCAGTACTTAA+CGG 0.452401 1:+1386431 None:intergenic
GGTTCTTACTTGAGAGCATC+GGG 0.471713 1:+1386853 None:intergenic
AGAAGCTGCTCAATAAATTG+AGG 0.480348 1:-1386512 MsG0180000096.01.T01:CDS
TCGCGCGATGAAGGTCTATG+AGG 0.510907 1:-1386917 None:intergenic
GAAGCTGCTCAATAAATTGA+GGG 0.519049 1:-1386511 MsG0180000096.01.T01:CDS
AACACTACTGGAAAGTAAGT+TGG 0.529696 1:+1386254 None:intergenic
TGGTTCTTACTTGAGAGCAT+CGG 0.539745 1:+1386852 None:intergenic
TTCTTGTTTATAGGAGCTGG+AGG 0.546787 1:-1386574 MsG0180000096.01.T01:intron
GGACACAAATACTGCTTGCT+CGG 0.550212 1:-1386722 MsG0180000096.01.T01:CDS
CTCAGTACTTAACGGGAGCA+AGG 0.561409 1:+1386439 None:intergenic
AGTTGTCATCAGAGGTTGGA+TGG 0.565112 1:-1386697 MsG0180000096.01.T01:CDS
TTGATCTTGTCATATGGAGG+AGG 0.574582 1:+1386889 None:intergenic
CTCCATATGACAAGATCAAG+AGG 0.577162 1:-1386885 MsG0180000096.01.T01:CDS
TCAGAGGTTGGATGGAACTA+CGG 0.586349 1:-1386689 MsG0180000096.01.T01:CDS
AATATATGATGTTACACCAA+AGG 0.587013 1:+1386228 None:intergenic
GGAGCTGGAGGATAAGAGGA+AGG 0.587458 1:-1386562 MsG0180000096.01.T01:CDS
GAACTACGGTGGCATCATCA+AGG 0.590785 1:-1386675 MsG0180000096.01.T01:intron
GGACAGTTGTCATCAGAGGT+TGG 0.592395 1:-1386701 MsG0180000096.01.T01:CDS
CTCTTGATCTTGTCATATGG+AGG 0.593959 1:+1386886 None:intergenic
GCTCGGACAGTTGTCATCAG+AGG 0.597789 1:-1386705 MsG0180000096.01.T01:CDS
AGGGTGTTGAGACTTCAAAA+GGG 0.620018 1:-1386743 MsG0180000096.01.T01:CDS
ATATGACAAGATCAAGAGGA+TGG 0.634497 1:-1386881 MsG0180000096.01.T01:CDS
GGAGCAAGGACTTCAAGTTG+GGG 0.640419 1:+1386453 None:intergenic
AGCAAGGACTTCAAGTTGGG+GGG 0.650449 1:+1386455 None:intergenic
GATAAGAGGAAGGAACGCGC+AGG 0.659068 1:-1386552 MsG0180000096.01.T01:CDS
GAGCAAGGACTTCAAGTTGG+GGG 0.688692 1:+1386454 None:intergenic
TATATGATGTTACACCAAAG+GGG 0.699513 1:+1386230 None:intergenic
GAGGTTGGATGGAACTACGG+TGG 0.706495 1:-1386686 MsG0180000096.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTGTTAACATTATTATA+AGG - Chr1:1386666-1386685 MsG0180000096.01.T01:intron 10.0%
!! CTGTAAATTATACAATAAAA+TGG + Chr1:1386250-1386269 None:intergenic 15.0%
!! CATTATTATAAGGTTTAGTT+TGG - Chr1:1386676-1386695 MsG0180000096.01.T01:CDS 20.0%
!!! ACTTTTGTTGTTGAATTAAA+AGG - Chr1:1386275-1386294 MsG0180000096.01.T01:three_prime_UTR 20.0%
!!! GTGATTTTTTTTGTTTTGTT+TGG - Chr1:1386426-1386445 MsG0180000096.01.T01:exon 20.0%
!!! GTGGTTTTGAATTTTTTAAT+TGG - Chr1:1386481-1386500 MsG0180000096.01.T01:CDS 20.0%
!!! TTAATTGGTTTCTTGTTTAT+AGG - Chr1:1386496-1386515 MsG0180000096.01.T01:CDS 20.0%
!!! TTCCAAAATTGTCATTTTTA+AGG + Chr1:1386767-1386786 None:intergenic 20.0%
! AAATAGTACTCAGTACTTAA+CGG + Chr1:1386651-1386670 None:intergenic 25.0%
! AATAACAAGCAAAACTGAAA+TGG + Chr1:1386730-1386749 None:intergenic 25.0%
! AATATATGATGTTACACCAA+AGG + Chr1:1386854-1386873 None:intergenic 25.0%
! ATATATGATGTTACACCAAA+GGG + Chr1:1386853-1386872 None:intergenic 25.0%
! TGAATGAGTGAATGAAATTA+GGG - Chr1:1386317-1386336 MsG0180000096.01.T01:three_prime_UTR 25.0%
!! GAATTTGATCAACAATTTTG+TGG - Chr1:1386462-1386481 MsG0180000096.01.T01:CDS 25.0%
!! GACCTTAAAAATGACAATTT+TGG - Chr1:1386762-1386781 MsG0180000096.01.T01:intron 25.0%
AATAGTACTCAGTACTTAAC+GGG + Chr1:1386650-1386669 None:intergenic 30.0%
ACTATGAAGCTTATAGTTGT+TGG - Chr1:1386897-1386916 MsG0180000096.01.T01:CDS 30.0%
CTGAATGAGTGAATGAAATT+AGG - Chr1:1386316-1386335 MsG0180000096.01.T01:three_prime_UTR 30.0%
TATATGATGTTACACCAAAG+GGG + Chr1:1386852-1386871 None:intergenic 30.0%
!! GCTTGTTATTCTATGTTTGT+TGG - Chr1:1386740-1386759 MsG0180000096.01.T01:CDS 30.0%
AACACTACTGGAAAGTAAGT+TGG + Chr1:1386828-1386847 None:intergenic 35.0%
AGAAGCTGCTCAATAAATTG+AGG - Chr1:1386567-1386586 MsG0180000096.01.T01:intron 35.0%
ATATGACAAGATCAAGAGGA+TGG - Chr1:1386198-1386217 MsG0180000096.01.T01:three_prime_UTR 35.0%
ATCCTCTTGATCTTGTCATA+TGG + Chr1:1386199-1386218 None:intergenic 35.0%
GAAGCTGCTCAATAAATTGA+GGG - Chr1:1386568-1386587 MsG0180000096.01.T01:intron 35.0%
CTCCATATGACAAGATCAAG+AGG - Chr1:1386194-1386213 MsG0180000096.01.T01:three_prime_UTR 40.0%
CTCTTGATCTTGTCATATGG+AGG + Chr1:1386196-1386215 None:intergenic 40.0%
TTGATCTTGTCATATGGAGG+AGG + Chr1:1386193-1386212 None:intergenic 40.0%
! GGTTTCTTGTTTATAGGAGC+TGG - Chr1:1386502-1386521 MsG0180000096.01.T01:CDS 40.0%
! TGGTTCTTACTTGAGAGCAT+CGG + Chr1:1386230-1386249 None:intergenic 40.0%
! TTCTTGTTTATAGGAGCTGG+AGG - Chr1:1386505-1386524 MsG0180000096.01.T01:CDS 40.0%
!! AGGGTGTTGAGACTTCAAAA+GGG - Chr1:1386336-1386355 MsG0180000096.01.T01:three_prime_UTR 40.0%
!! TAGGGTGTTGAGACTTCAAA+AGG - Chr1:1386335-1386354 MsG0180000096.01.T01:three_prime_UTR 40.0%
AGTTGTCATCAGAGGTTGGA+TGG - Chr1:1386382-1386401 MsG0180000096.01.T01:three_prime_UTR 45.0%
GGACACAAATACTGCTTGCT+CGG - Chr1:1386357-1386376 MsG0180000096.01.T01:three_prime_UTR 45.0%
TATAGGAGCTGGAGGATAAG+AGG - Chr1:1386513-1386532 MsG0180000096.01.T01:CDS 45.0%
TCAGAGGTTGGATGGAACTA+CGG - Chr1:1386390-1386409 MsG0180000096.01.T01:three_prime_UTR 45.0%
!! GGTTCTTACTTGAGAGCATC+GGG + Chr1:1386229-1386248 None:intergenic 45.0%
CTCAGTACTTAACGGGAGCA+AGG + Chr1:1386643-1386662 None:intergenic 50.0%
GAACTACGGTGGCATCATCA+AGG - Chr1:1386404-1386423 MsG0180000096.01.T01:three_prime_UTR 50.0%
GGACAGTTGTCATCAGAGGT+TGG - Chr1:1386378-1386397 MsG0180000096.01.T01:three_prime_UTR 50.0%
TTCCAGTAGTGTTGCCCCTT+TGG - Chr1:1386835-1386854 MsG0180000096.01.T01:intron 50.0%
! GGGAGCAAGGACTTCAAGTT+GGG + Chr1:1386630-1386649 None:intergenic 50.0%
!! AGCAAGGACTTCAAGTTGGG+GGG + Chr1:1386627-1386646 None:intergenic 50.0%
!! GAGCAAGGACTTCAAGTTGG+GGG + Chr1:1386628-1386647 None:intergenic 50.0%
!! GGAGCAAGGACTTCAAGTTG+GGG + Chr1:1386629-1386648 None:intergenic 50.0%
CACCAAAGGGGCAACACTAC+TGG + Chr1:1386840-1386859 None:intergenic 55.0%
CGGGAGCAAGGACTTCAAGT+TGG + Chr1:1386631-1386650 None:intergenic 55.0%
GAGGTTGGATGGAACTACGG+TGG - Chr1:1386393-1386412 MsG0180000096.01.T01:three_prime_UTR 55.0%
GATAAGAGGAAGGAACGCGC+AGG - Chr1:1386527-1386546 MsG0180000096.01.T01:CDS 55.0%
GCTCGGACAGTTGTCATCAG+AGG - Chr1:1386374-1386393 MsG0180000096.01.T01:three_prime_UTR 55.0%
GGAGCTGGAGGATAAGAGGA+AGG - Chr1:1386517-1386536 MsG0180000096.01.T01:CDS 55.0%
GAGGAAGGAACGCGCAGGTT+TGG - Chr1:1386532-1386551 MsG0180000096.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 1386169 1386932 1386169 ID=MsG0180000096.01;Name=MsG0180000096.01
Chr1 mRNA 1386169 1386932 1386169 ID=MsG0180000096.01.T01;Parent=MsG0180000096.01;Name=MsG0180000096.01.T01;_AED=0.44;_eAED=0.44;_QI=0|1|1|1|0.66|0.5|4|271|100
Chr1 exon 1386862 1386932 1386862 ID=MsG0180000096.01.T01:exon:7287;Parent=MsG0180000096.01.T01
Chr1 exon 1386676 1386763 1386676 ID=MsG0180000096.01.T01:exon:7288;Parent=MsG0180000096.01.T01
Chr1 exon 1386169 1386583 1386169 ID=MsG0180000096.01.T01:exon:7289;Parent=MsG0180000096.01.T01
Chr1 CDS 1386862 1386932 1386862 ID=MsG0180000096.01.T01:cds;Parent=MsG0180000096.01.T01
Chr1 CDS 1386676 1386763 1386676 ID=MsG0180000096.01.T01:cds;Parent=MsG0180000096.01.T01
Chr1 CDS 1386440 1386583 1386440 ID=MsG0180000096.01.T01:cds;Parent=MsG0180000096.01.T01
Chr1 three_prime_UTR 1386169 1386439 1386169 ID=MsG0180000096.01.T01:three_prime_utr;Parent=MsG0180000096.01.T01
Gene Sequence

>MsG0180000096.01.T01

ATGAAGGTCTATGAGGGTATTCCTCCTCCATATGACAAGATCAAGAGGATGGTTGTTCCCGATGCTCTCAAGGTGTTGAGACTTCAAAAGGGACACAAATACTGCTTGCTCGGACAGTTGTCATCAGAGGTTGGATGGAACTACGGTGGCATCATCAAGGAGCTGGAGGATAAGAGGAAGGAACGCGCAGGTTTGGCTTACGAGAAAAAGAAGCTGCTCAATAAATTGAGGGCTAAAGCTGAGAAGATTGTAGACGAGAAGCTTGCCCCCCAACTTGAAGTCCTTGCTCCCGTTAAGTACTGA

Protein sequence

>MsG0180000096.01.T01

MKVYEGIPPPYDKIKRMVVPDALKVLRLQKGHKYCLLGQLSSEVGWNYGGIIKELEDKRKERAGLAYEKKKLLNKLRAKAEKIVDEKLAPQLEVLAPVKY*