AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006367.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006367.01.T01 MTR_2g007010 79.268 82 13 1 1 82 104 181 4.61e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATTCCTTCTTTGTTTC+CGG 0.154684 2:-949489 None:intergenic
TTAAGAAAAGAATGTTGAAT+TGG 0.360696 2:+949507 MsG0280006367.01.T01:CDS
TGAAGAGAAATTGCAAAGAT+TGG 0.368516 2:+949286 MsG0280006367.01.T01:CDS
AAGAATGTTGAATTGGATAT+AGG 0.396387 2:+949514 MsG0280006367.01.T01:CDS
TCTTCTTCTGATGATGATGA+AGG 0.451469 2:+949377 MsG0280006367.01.T01:CDS
TCCTTCTTTGTTTCCGGTGT+AGG 0.471534 2:-949483 None:intergenic
TCCTACACCGGAAACAAAGA+AGG 0.546267 2:+949482 MsG0280006367.01.T01:CDS
TCTTTCATCAAATCAAAAGC+AGG 0.560821 2:-949446 None:intergenic
AGAAGAAGAATCTTCTTCAG+AGG 0.609927 2:-949360 None:intergenic
AAGCTCGTCACATTGAAAAG+AGG 0.619710 2:+949309 MsG0280006367.01.T01:CDS
AGGTGCAGTTTCGACTTCAG+AGG 0.632200 2:+949397 MsG0280006367.01.T01:CDS
ATGCTTAGAAGTTCCTACAC+CGG 0.667364 2:+949470 MsG0280006367.01.T01:CDS
TTGAAAAGAGGATGAACACG+CGG 0.691387 2:+949321 MsG0280006367.01.T01:CDS
TGAAAAGAGGATGAACACGC+GGG 0.720971 2:+949322 MsG0280006367.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGAAAAGAATGTTGAAT+TGG + Chr2:949507-949526 MsG0280006367.01.T01:CDS 20.0%
TCTTTCATCAAATCAAAAGC+AGG - Chr2:949449-949468 None:intergenic 30.0%
TGAAGAGAAATTGCAAAGAT+TGG + Chr2:949286-949305 MsG0280006367.01.T01:CDS 30.0%
! CTTAATTCCTTCTTTGTTTC+CGG - Chr2:949492-949511 None:intergenic 30.0%
AGAAGAAGAATCTTCTTCAG+AGG - Chr2:949363-949382 None:intergenic 35.0%
! TCTTCTTCTGATGATGATGA+AGG + Chr2:949377-949396 MsG0280006367.01.T01:CDS 35.0%
AAGCTCGTCACATTGAAAAG+AGG + Chr2:949309-949328 MsG0280006367.01.T01:CDS 40.0%
ATGCTTAGAAGTTCCTACAC+CGG + Chr2:949470-949489 MsG0280006367.01.T01:CDS 40.0%
TTGAAAAGAGGATGAACACG+CGG + Chr2:949321-949340 MsG0280006367.01.T01:CDS 40.0%
TCCTACACCGGAAACAAAGA+AGG + Chr2:949482-949501 MsG0280006367.01.T01:CDS 45.0%
TGAAAAGAGGATGAACACGC+GGG + Chr2:949322-949341 MsG0280006367.01.T01:CDS 45.0%
! TCCTTCTTTGTTTCCGGTGT+AGG - Chr2:949486-949505 None:intergenic 45.0%
AGGTGCAGTTTCGACTTCAG+AGG + Chr2:949397-949416 MsG0280006367.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 949272 949535 949272 ID=MsG0280006367.01;Name=MsG0280006367.01
Chr2 mRNA 949272 949535 949272 ID=MsG0280006367.01.T01;Parent=MsG0280006367.01;Name=MsG0280006367.01.T01;_AED=0.48;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|87
Chr2 exon 949272 949535 949272 ID=MsG0280006367.01.T01:exon:954;Parent=MsG0280006367.01.T01
Chr2 CDS 949272 949535 949272 ID=MsG0280006367.01.T01:cds;Parent=MsG0280006367.01.T01
Gene Sequence

>MsG0280006367.01.T01

ATGATCGATGAATCTGAAGAGAAATTGCAAAGATTGGAAGCTCGTCACATTGAAAAGAGGATGAACACGCGGGTTGAATCATCATCATCCTCTGAAGAAGATTCTTCTTCTTCTGATGATGATGAAGGTGCAGTTTCGACTTCAGAGGATGCAATTTATAGTGAGAAAGTTGAACCTGCTTTTGATTTGATGAAAGATATGCTTAGAAGTTCCTACACCGGAAACAAAGAAGGAATTAAGAAAAGAATGTTGAATTGGATATAG

Protein sequence

>MsG0280006367.01.T01

MIDESEEKLQRLEARHIEKRMNTRVESSSSSEEDSSSSDDDEGAVSTSEDAIYSEKVEPAFDLMKDMLRSSYTGNKEGIKKRMLNWI*