AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008864.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008864.01.T01 MTR_2g449830 95.939 197 8 0 87 283 224 420 2.13e-137 394
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008864.01.T01 AT2G22650 65.104 192 66 1 87 277 103 294 6.41e-92 274
MsG0280008864.01.T01 AT2G22650 65.104 192 66 1 87 277 103 294 6.41e-92 274
MsG0280008864.01.T01 AT2G22650 65.104 192 66 1 87 277 225 416 4.28e-90 274

Find 88 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAATTACTTCTTCATTAC+AGG 0.181074 2:-40180863 None:intergenic
CCCTAGGTGTCTTTAAATTA+AGG 0.182750 2:+40180988 MsG0280008863.01.T01:exon
AGTTGTAAGTATTGGTTATT+TGG 0.214020 2:+40180772 MsG0280008863.01.T01:exon
AATATGCTTCCTGAGATTTC+TGG 0.214703 2:+40180189 MsG0280008863.01.T01:CDS
ACATGGATCGCTTCCACTTC+TGG 0.214988 2:+40180717 MsG0280008863.01.T01:exon
GACAATTGAATTCTAAAGTT+TGG 0.265520 2:-40180917 None:intergenic
AGGAAGATGGGCCTTTATTA+AGG 0.282178 2:+40178592 MsG0280008863.01.T01:intron
AATCCATGCATCTGATAATA+TGG 0.287035 2:-40180491 None:intergenic
ATAACCAATACTTACAACTA+TGG 0.303357 2:-40180768 None:intergenic
GTAACACCATCAAATGGTTT+TGG 0.324237 2:-40178657 None:intergenic
GGTATCGCTCAGGCAGAATT+TGG 0.328740 2:+40178747 MsG0280008863.01.T01:intron
GAAAACCCAATCCTTTATTC+CGG 0.342382 2:-40180650 None:intergenic
CCTCCACCAAAACCATTTGA+TGG 0.345181 2:+40178651 MsG0280008863.01.T01:intron
TCGTATAATCAGGCGGTTCC+AGG 0.351981 2:-40180251 None:intergenic
AGCGTCGAAAACAATCTTTA+AGG 0.362210 2:+40178721 MsG0280008863.01.T01:intron
CATGGATCGCTTCCACTTCT+GGG 0.364062 2:+40180718 MsG0280008863.01.T01:exon
AGTCATTTATCCCGGAATAA+AGG 0.366833 2:+40180639 MsG0280008863.01.T01:exon
AGATGCATGGATTTCTGTTC+AGG 0.375339 2:+40180501 MsG0280008863.01.T01:exon
AAACTTTACCTCGTATAATC+AGG 0.377195 2:-40180261 None:intergenic
GTCTAAATGTTGGGTCAACT+TGG 0.393363 2:+40180533 MsG0280008863.01.T01:exon
CACAGCTCCATCCTTAATAA+AGG 0.407722 2:-40178603 None:intergenic
ATATGCTTCCTGAGATTTCT+GGG 0.417682 2:+40180190 MsG0280008863.01.T01:CDS
AGGCAGAATTTGGTAGAAAC+TGG 0.427719 2:+40178757 MsG0280008863.01.T01:intron
TCAAGAGGATTGTTATATCA+TGG 0.430914 2:+40180805 MsG0280008863.01.T01:exon
TGATAGGACTCGAACAAGTA+AGG 0.437485 2:+40179512 MsG0280008863.01.T01:intron
TTCCATCTGAGTTGACTACT+TGG 0.437844 2:+40180881 MsG0280008863.01.T01:exon
GGTTGTATTAACGATTTCAT+TGG 0.438305 2:+40180739 MsG0280008863.01.T01:exon
TAAGTATATTCAAATTCAAA+TGG 0.441820 2:+40181054 MsG0280008863.01.T01:exon
GGCCCATCTTCCTTTGCTGC+TGG 0.449493 2:-40178582 None:intergenic
TATTGGTTATTTGGAGGACT+AGG 0.452045 2:+40180781 MsG0280008863.01.T01:exon
TTTATCCCGGAATAAAGGAT+TGG 0.453765 2:+40180644 MsG0280008863.01.T01:exon
TGGTTGGCTAGGTAGTTTGA+TGG 0.454075 2:+40180825 MsG0280008863.01.T01:exon
GAAACCATAGTTGTAAGTAT+TGG 0.454184 2:+40180764 MsG0280008863.01.T01:exon
TTATCCCGGAATAAAGGATT+GGG 0.454396 2:+40180645 MsG0280008863.01.T01:exon
AAAACCCAATCCTTTATTCC+GGG 0.457063 2:-40180649 None:intergenic
AAGGATGGAGCTGTGGGTCC+TGG 0.474922 2:+40178611 MsG0280008863.01.T01:intron
TGTAAGTATTGGTTATTTGG+AGG 0.485239 2:+40180775 MsG0280008863.01.T01:exon
TGGAGCAAAAGCTGGTTTGA+GGG 0.487851 2:+40180675 MsG0280008863.01.T01:exon
CTCAGGAAGCATATTCACTT+TGG 0.488224 2:-40180181 None:intergenic
TTCAGGTGTTATCCTGAGCA+TGG 0.490494 2:+40180463 MsG0280008863.01.T01:intron
CTGGAGCAAAAGCTGGTTTG+AGG 0.492766 2:+40180674 MsG0280008863.01.T01:exon
GATGCATGGATTTCTGTTCA+GGG 0.493865 2:+40180502 MsG0280008863.01.T01:exon
GAAACTTTGTTATAATGTCC+AGG 0.495854 2:-40178629 None:intergenic
GGATAAATGACTGATGCCTT+CGG 0.496258 2:-40180628 None:intergenic
CTTTATTAAGGATGGAGCTG+TGG 0.502149 2:+40178604 MsG0280008863.01.T01:intron
GTGTTAGTAACACCATCAAA+TGG 0.504782 2:-40178663 None:intergenic
CAGAGGTGTAATTGTTCACC+TGG 0.510119 2:+40180233 MsG0280008863.01.T01:CDS
AGATGGGCCTTTATTAAGGA+TGG 0.515908 2:+40178596 MsG0280008863.01.T01:intron
GGCTCCCGCAGTCTAAATGT+TGG 0.516611 2:+40180523 MsG0280008863.01.T01:exon
GAGGATTGTTATATCATGGT+TGG 0.516934 2:+40180809 MsG0280008863.01.T01:exon
GCTCCCGCAGTCTAAATGTT+GGG 0.519128 2:+40180524 MsG0280008863.01.T01:exon
AACTTGTATGTGTATTGTTG+TGG 0.520500 2:+40179537 MsG0280008863.01.T01:intron
TTGTTATATCATGGTTGGCT+AGG 0.521087 2:+40180814 MsG0280008863.01.T01:exon
TTTATTAAGGATGGAGCTGT+GGG 0.523672 2:+40178605 MsG0280008863.01.T01:intron
CTTCCATATTATCAGATGCA+TGG 0.523672 2:+40180488 MsG0280008863.01.T01:exon
ATACCAGCAGCAAAGGAAGA+TGG 0.527140 2:+40178579 MsG0280008863.01.T01:intron
GGAAGCCTCCCAGAAATCTC+AGG 0.533218 2:-40180198 None:intergenic
TTCCAAGTAGTCAACTCAGA+TGG 0.537769 2:-40180883 None:intergenic
TGACCCAACATTTAGACTGC+GGG 0.542533 2:-40180527 None:intergenic
AATATGGAAGGTCCATGCTC+AGG 0.543184 2:-40180475 None:intergenic
TACCAGCAGCAAAGGAAGAT+GGG 0.549737 2:+40178580 MsG0280008863.01.T01:intron
AAGGATGATACCAGCAGCAA+AGG 0.557818 2:+40178572 MsG0280008863.01.T01:intron
TTTAAGGAGTGGTATCGCTC+AGG 0.562400 2:+40178737 MsG0280008863.01.T01:intron
GATAGGACTCGAACAAGTAA+GGG 0.567547 2:+40179513 MsG0280008863.01.T01:intron
TTTGGAGGACTAGGTTCAAG+AGG 0.570574 2:+40180790 MsG0280008863.01.T01:exon
ATGTTGGGTCAACTTGGGAG+TGG 0.575664 2:+40180539 MsG0280008863.01.T01:exon
AGCGATCCATGTGGCGTGAG+TGG 0.578662 2:-40180706 None:intergenic
AAGGCATCAGTCATTTATCC+CGG 0.588732 2:+40180631 MsG0280008863.01.T01:exon
CGAAAACAATCTTTAAGGAG+TGG 0.588886 2:+40178726 MsG0280008863.01.T01:intron
ACACCTCTGCATGTTCTCAA+AGG 0.589315 2:-40180219 None:intergenic
TCGTTAATACAACCCAGAAG+TGG 0.592302 2:-40180730 None:intergenic
GATCCATGTGGCGTGAGTGG+AGG 0.596844 2:-40180703 None:intergenic
TCTAAATGTTGGGTCAACTT+GGG 0.597738 2:+40180534 MsG0280008863.01.T01:exon
GCATATTCACTTTGGCACCA+AGG 0.598392 2:-40180173 None:intergenic
ATGCCTCCACTCACGCCACA+TGG 0.599791 2:+40180700 MsG0280008863.01.T01:exon
CATGCATCTGATAATATGGA+AGG 0.608929 2:-40180487 None:intergenic
CCTTAATTTAAAGACACCTA+GGG 0.611738 2:-40180988 None:intergenic
ACCTTAATTTAAAGACACCT+AGG 0.614282 2:-40180989 None:intergenic
GATGCAGTCATTATATGCCT+TGG 0.621755 2:+40180156 MsG0280008863.01.T01:CDS
TCTTCATCAGCTACTGCCGA+AGG 0.624818 2:+40180612 MsG0280008863.01.T01:exon
CTTTACCTCGTATAATCAGG+CGG 0.650780 2:-40180258 None:intergenic
TTGACCCAACATTTAGACTG+CGG 0.672778 2:-40180528 None:intergenic
CTTCCTTTGAGAACATGCAG+AGG 0.679214 2:+40180216 MsG0280008863.01.T01:CDS
TGCTTCCTGAGATTTCTGGG+AGG 0.708278 2:+40180193 MsG0280008863.01.T01:CDS
AAGGTTGTGCAATTTCATCG+TGG 0.730410 2:-40178697 None:intergenic
TGGAACCGCCTGATTATACG+AGG 0.741048 2:+40180253 MsG0280008863.01.T01:CDS
AGAAGTGGAAGCGATCCATG+TGG 0.754617 2:-40180715 None:intergenic
TGATGGTGTTACTAACACAG+TGG 0.825493 2:+40178668 MsG0280008863.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATTAATAGTAATTAAAA+TGG + Chr2:40178790-40178809 MsG0280008863.01.T01:intron 10.0%
!! TAAGTATATTCAAATTCAAA+TGG + Chr2:40181054-40181073 MsG0280008863.01.T01:exon 15.0%
!! TTTATGCATTGTAATATATA+AGG - Chr2:40178884-40178903 None:intergenic 15.0%
!!! ATAATTAGTTTGAAGAAATA+AGG + Chr2:40179276-40179295 MsG0280008863.01.T01:intron 15.0%
!!! ATATATTTTATATTTTCCCT+AGG + Chr2:40180972-40180991 MsG0280008863.01.T01:exon 15.0%
!! AAAATACAACCAAACTATAA+AGG + Chr2:40179604-40179623 MsG0280008863.01.T01:intron 20.0%
!! ACTCAAAAAATAATGCAAAA+TGG - Chr2:40181016-40181035 None:intergenic 20.0%
!! AGCATAGTATATTTAAATGA+AGG - Chr2:40180393-40180412 None:intergenic 20.0%
!! TAACAATAACTAACAAAGTA+AGG - Chr2:40179235-40179254 None:intergenic 20.0%
!! TATAGAAACTTAATCAGAAA+AGG - Chr2:40179416-40179435 None:intergenic 20.0%
!! TGTAATATATAAGGAAGTAA+TGG - Chr2:40178875-40178894 None:intergenic 20.0%
!! TTACTTTGTTAGTTATTGTT+AGG + Chr2:40179234-40179253 MsG0280008863.01.T01:intron 20.0%
! AATCTGAAAAATCTCTAGTT+TGG + Chr2:40180310-40180329 MsG0280008863.01.T01:intron 25.0%
! AATTAGTGAGATTAGAATAG+GGG + Chr2:40179344-40179363 MsG0280008863.01.T01:intron 25.0%
! AGAACAAAAACATAGGAATA+CGG + Chr2:40179169-40179188 MsG0280008863.01.T01:intron 25.0%
! AGAATCAAAGCAATACATTA+TGG - Chr2:40179791-40179810 None:intergenic 25.0%
! ATAACCAATACTTACAACTA+TGG - Chr2:40180771-40180790 None:intergenic 25.0%
! ATGCCTGAAAATAATAATAG+GGG + Chr2:40179303-40179322 MsG0280008863.01.T01:intron 25.0%
! ATTGGATTCAAGTATTCATA+TGG + Chr2:40179457-40179476 MsG0280008863.01.T01:intron 25.0%
! CAATTAGTGAGATTAGAATA+GGG + Chr2:40179343-40179362 MsG0280008863.01.T01:intron 25.0%
! GAATCAAAGCAATACATTAT+GGG - Chr2:40179790-40179809 None:intergenic 25.0%
! GATGCCTGAAAATAATAATA+GGG + Chr2:40179302-40179321 MsG0280008863.01.T01:intron 25.0%
! TCACTAGTATGAAAAATGAT+AGG - Chr2:40180020-40180039 None:intergenic 25.0%
! TGATGCCTGAAAATAATAAT+AGG + Chr2:40179301-40179320 MsG0280008863.01.T01:intron 25.0%
!! AATGCAAGATGATTCTAAAA+TGG - Chr2:40180439-40180458 None:intergenic 25.0%
!! TCATTCAGTTATTTCGATAA+AGG - Chr2:40178841-40178860 None:intergenic 25.0%
!! TCTTTGTTTCCTTTATAGTT+TGG - Chr2:40179616-40179635 None:intergenic 25.0%
!! TTAAATATCTTTCAAGTCTC+AGG + Chr2:40180126-40180145 MsG0280008863.01.T01:intron 25.0%
!!! AGTTGTAAGTATTGGTTATT+TGG + Chr2:40180772-40180791 MsG0280008863.01.T01:exon 25.0%
!!! ATGCAAGATGATTCTAAAAT+GGG - Chr2:40180438-40180457 None:intergenic 25.0%
!!! GACAATTGAATTCTAAAGTT+TGG - Chr2:40180920-40180939 None:intergenic 25.0%
!!! TGCATTATTTTTTGAGTGAA+AGG + Chr2:40181019-40181038 MsG0280008863.01.T01:exon 25.0%
AAAACTGAACACATAACTGT+CGG + Chr2:40178916-40178935 MsG0280008863.01.T01:intron 30.0%
AAACTTTACCTCGTATAATC+AGG - Chr2:40180264-40180283 None:intergenic 30.0%
AACTTGTATGTGTATTGTTG+TGG + Chr2:40179537-40179556 MsG0280008863.01.T01:intron 30.0%
AATCCATGCATCTGATAATA+TGG - Chr2:40180494-40180513 None:intergenic 30.0%
AATTGAGAGAATTGGTATTG+AGG - Chr2:40179739-40179758 None:intergenic 30.0%
ACCTTAATTTAAAGACACCT+AGG - Chr2:40180992-40181011 None:intergenic 30.0%
ACGTCAGTGAGAATATTTAT+TGG + Chr2:40179439-40179458 MsG0280008863.01.T01:intron 30.0%
ATATAAGGAAGTAATGGCAA+AGG - Chr2:40178869-40178888 None:intergenic 30.0%
CCTTAATTTAAAGACACCTA+GGG - Chr2:40180991-40181010 None:intergenic 30.0%
CTCTCCTATATTGTGTATTA+CGG + Chr2:40179993-40180012 MsG0280008863.01.T01:intron 30.0%
CTTTGTTAGTTATTGTTAGG+TGG + Chr2:40179237-40179256 MsG0280008863.01.T01:intron 30.0%
GAAACCATAGTTGTAAGTAT+TGG + Chr2:40180764-40180783 MsG0280008863.01.T01:exon 30.0%
GAAGTAAAGCTACTTAAAGA+TGG + Chr2:40179645-40179664 MsG0280008863.01.T01:intron 30.0%
GCAATTAGTGAGATTAGAAT+AGG + Chr2:40179342-40179361 MsG0280008863.01.T01:intron 30.0%
GGAATGAACAAAACTAAAGA+AGG + Chr2:40179671-40179690 MsG0280008863.01.T01:intron 30.0%
GGTTGTATTAACGATTTCAT+TGG + Chr2:40180739-40180758 MsG0280008863.01.T01:exon 30.0%
GTATTCATATGGCATAATGA+AGG + Chr2:40179468-40179487 MsG0280008863.01.T01:intron 30.0%
TATAAGGAAGTAATGGCAAA+GGG - Chr2:40178868-40178887 None:intergenic 30.0%
TATGGGAAGTAACAAAATGA+GGG - Chr2:40179773-40179792 None:intergenic 30.0%
TCAAGAGGATTGTTATATCA+TGG + Chr2:40180805-40180824 MsG0280008863.01.T01:exon 30.0%
TCTCCTATATTGTGTATTAC+GGG + Chr2:40179994-40180013 MsG0280008863.01.T01:intron 30.0%
TGGAATTACTTCTTCATTAC+AGG - Chr2:40180866-40180885 None:intergenic 30.0%
TGTTGTGACTTAGATAACAA+AGG + Chr2:40179122-40179141 MsG0280008863.01.T01:intron 30.0%
TTATGGGAAGTAACAAAATG+AGG - Chr2:40179774-40179793 None:intergenic 30.0%
TTCTGTTAGACATGTAGTTA+AGG - Chr2:40179002-40179021 None:intergenic 30.0%
! AGTAGCTTTACTTCAAGTAA+CGG - Chr2:40179639-40179658 None:intergenic 30.0%
! ATGTTTCAACGTTTTCTGAT+AGG + Chr2:40179496-40179515 MsG0280008863.01.T01:intron 30.0%
! ATTGAGAGAATTGGTATTGA+GGG - Chr2:40179738-40179757 None:intergenic 30.0%
! GAAACTTTGTTATAATGTCC+AGG - Chr2:40178632-40178651 None:intergenic 30.0%
! GAACCCCTATTATTATTTTC+AGG - Chr2:40179309-40179328 None:intergenic 30.0%
! TCTAAAATGGGATATGACTT+TGG - Chr2:40180426-40180445 None:intergenic 30.0%
!! GTATTCCTATGTTTTTGTTC+TGG - Chr2:40179170-40179189 None:intergenic 30.0%
!! TGTAAGTATTGGTTATTTGG+AGG + Chr2:40180775-40180794 MsG0280008863.01.T01:exon 30.0%
!!! AAGAGTATTGTTTTCATGGA+AGG + Chr2:40180365-40180384 MsG0280008863.01.T01:intron 30.0%
!!! AGAGTATTGTTTTCATGGAA+GGG + Chr2:40180366-40180385 MsG0280008863.01.T01:intron 30.0%
!!! CTTCAAGAGTATTGTTTTCA+TGG + Chr2:40180361-40180380 MsG0280008863.01.T01:intron 30.0%
AAAACCCAATCCTTTATTCC+GGG - Chr2:40180652-40180671 None:intergenic 35.0%
AAAGAAGCGATAGAGGAATA+AGG + Chr2:40179205-40179224 MsG0280008863.01.T01:intron 35.0%
AAAGCTACTTAAAGATGGAC+AGG + Chr2:40179650-40179669 MsG0280008863.01.T01:intron 35.0%
AATATGCTTCCTGAGATTTC+TGG + Chr2:40180189-40180208 MsG0280008863.01.T01:CDS 35.0%
AGGGAAACAGATGTATGAAA+TGG - Chr2:40179880-40179899 None:intergenic 35.0%
AGTCATTTATCCCGGAATAA+AGG + Chr2:40180639-40180658 MsG0280008863.01.T01:exon 35.0%
ATATGCTTCCTGAGATTTCT+GGG + Chr2:40180190-40180209 MsG0280008863.01.T01:CDS 35.0%
ATCATCTTGCATTGTCTTTC+AGG + Chr2:40180446-40180465 MsG0280008863.01.T01:intron 35.0%
ATTCCAAAAAGAAGCGATAG+AGG + Chr2:40179198-40179217 MsG0280008863.01.T01:intron 35.0%
CATCTGAGAATTGAGAGAAT+TGG - Chr2:40179747-40179766 None:intergenic 35.0%
CATGCATCTGATAATATGGA+AGG - Chr2:40180490-40180509 None:intergenic 35.0%
CCCTAGGTGTCTTTAAATTA+AGG + Chr2:40180988-40181007 MsG0280008863.01.T01:exon 35.0%
CGTAATACACAATATAGGAG+AGG - Chr2:40179995-40180014 None:intergenic 35.0%
CTTCCATATTATCAGATGCA+TGG + Chr2:40180488-40180507 MsG0280008863.01.T01:exon 35.0%
GAAAACCCAATCCTTTATTC+CGG - Chr2:40180653-40180672 None:intergenic 35.0%
GACTTAGATAACAAAGGCAA+AGG + Chr2:40179128-40179147 MsG0280008863.01.T01:intron 35.0%
GAGGATTGTTATATCATGGT+TGG + Chr2:40180809-40180828 MsG0280008863.01.T01:exon 35.0%
GGAAAAAGAAATCTGATCAG+AGG + Chr2:40179558-40179577 MsG0280008863.01.T01:intron 35.0%
GGAAACAGATGTATGAAATG+GGG - Chr2:40179878-40179897 None:intergenic 35.0%
GGGAAACAGATGTATGAAAT+GGG - Chr2:40179879-40179898 None:intergenic 35.0%
GTAGTTAAGGAACTGTAGAA+GGG - Chr2:40178989-40179008 None:intergenic 35.0%
GTGTTAGTAACACCATCAAA+TGG - Chr2:40178666-40178685 None:intergenic 35.0%
TAGTTAAGGAACTGTAGAAG+GGG - Chr2:40178988-40179007 None:intergenic 35.0%
TCTAAATGTTGGGTCAACTT+GGG + Chr2:40180534-40180553 MsG0280008863.01.T01:exon 35.0%
TGTAGTTAAGGAACTGTAGA+AGG - Chr2:40178990-40179009 None:intergenic 35.0%
TTATCCCGGAATAAAGGATT+GGG + Chr2:40180645-40180664 MsG0280008863.01.T01:exon 35.0%
TTTATCCCGGAATAAAGGAT+TGG + Chr2:40180644-40180663 MsG0280008863.01.T01:exon 35.0%
TTTATTAAGGATGGAGCTGT+GGG + Chr2:40178605-40178624 MsG0280008863.01.T01:intron 35.0%
! AAGATTGTTTTCGACGCTTA+AGG - Chr2:40178719-40178738 None:intergenic 35.0%
! AGCGTCGAAAACAATCTTTA+AGG + Chr2:40178721-40178740 MsG0280008863.01.T01:intron 35.0%
! ATAAAGGATTGGGTTTTCAC+TGG + Chr2:40180655-40180674 MsG0280008863.01.T01:exon 35.0%
! ATTCCTCTATCGCTTCTTTT+TGG - Chr2:40179204-40179223 None:intergenic 35.0%
! CGAAAACAATCTTTAAGGAG+TGG + Chr2:40178726-40178745 MsG0280008863.01.T01:intron 35.0%
! GTAACACCATCAAATGGTTT+TGG - Chr2:40178660-40178679 None:intergenic 35.0%
! TAATAGGGGTTCTAATGAAG+AGG + Chr2:40179317-40179336 MsG0280008863.01.T01:intron 35.0%
! TATTGGTTATTTGGAGGACT+AGG + Chr2:40180781-40180800 MsG0280008863.01.T01:exon 35.0%
! TTGTTATATCATGGTTGGCT+AGG + Chr2:40180814-40180833 MsG0280008863.01.T01:exon 35.0%
!! TCAGATTGAAAGCATTTGTC+AGG - Chr2:40180297-40180316 None:intergenic 35.0%
AAGAGGAAGTAAAGAGGGTA+GGG - Chr2:40179899-40179918 None:intergenic 40.0%
AAGGTTGTGCAATTTCATCG+TGG - Chr2:40178700-40178719 None:intergenic 40.0%
AGAGGCAGAAACAACAGAAT+GGG - Chr2:40179852-40179871 None:intergenic 40.0%
AGATGCATGGATTTCTGTTC+AGG + Chr2:40180501-40180520 MsG0280008863.01.T01:exon 40.0%
AGATGGGCCTTTATTAAGGA+TGG + Chr2:40178596-40178615 MsG0280008863.01.T01:intron 40.0%
AGGAAGATGGGCCTTTATTA+AGG + Chr2:40178592-40178611 MsG0280008863.01.T01:intron 40.0%
AGGCAGAATTTGGTAGAAAC+TGG + Chr2:40178757-40178776 MsG0280008863.01.T01:intron 40.0%
AGGCCCGTAATACACAATAT+AGG - Chr2:40180000-40180019 None:intergenic 40.0%
ATGTATGAAATGGGGAAGAG+AGG - Chr2:40179870-40179889 None:intergenic 40.0%
CAATATAGGAGAGGAACAAG+TGG - Chr2:40179986-40180005 None:intergenic 40.0%
CACACAAGAGGAAGTAAAGA+GGG - Chr2:40179904-40179923 None:intergenic 40.0%
CACAGCTCCATCCTTAATAA+AGG - Chr2:40178606-40178625 None:intergenic 40.0%
CAGCTCCAGAACAAAAACAT+AGG + Chr2:40179162-40179181 MsG0280008863.01.T01:intron 40.0%
CTTTACCTCGTATAATCAGG+CGG - Chr2:40180261-40180280 None:intergenic 40.0%
CTTTATTAAGGATGGAGCTG+TGG + Chr2:40178604-40178623 MsG0280008863.01.T01:intron 40.0%
GAAAATGGGCCATCTGAAAA+GGG - Chr2:40179954-40179973 None:intergenic 40.0%
GAGAAACAATGTCACACAAG+AGG - Chr2:40179916-40179935 None:intergenic 40.0%
GATAGGACTCGAACAAGTAA+GGG + Chr2:40179513-40179532 MsG0280008863.01.T01:intron 40.0%
GATGCAGTCATTATATGCCT+TGG + Chr2:40180156-40180175 MsG0280008863.01.T01:CDS 40.0%
GATGCATGGATTTCTGTTCA+GGG + Chr2:40180502-40180521 MsG0280008863.01.T01:exon 40.0%
GGATAAATGACTGATGCCTT+CGG - Chr2:40180631-40180650 None:intergenic 40.0%
GTCTAAATGTTGGGTCAACT+TGG + Chr2:40180533-40180552 MsG0280008863.01.T01:exon 40.0%
TCACACAAGAGGAAGTAAAG+AGG - Chr2:40179905-40179924 None:intergenic 40.0%
TCGTTAATACAACCCAGAAG+TGG - Chr2:40180733-40180752 None:intergenic 40.0%
TGAAAATGGGCCATCTGAAA+AGG - Chr2:40179955-40179974 None:intergenic 40.0%
TGATAGGACTCGAACAAGTA+AGG + Chr2:40179512-40179531 MsG0280008863.01.T01:intron 40.0%
TTCCAAGTAGTCAACTCAGA+TGG - Chr2:40180886-40180905 None:intergenic 40.0%
TTGACCCAACATTTAGACTG+CGG - Chr2:40180531-40180550 None:intergenic 40.0%
! AAGGCATCAGTCATTTATCC+CGG + Chr2:40180631-40180650 MsG0280008863.01.T01:exon 40.0%
! CTCAGGAAGCATATTCACTT+TGG - Chr2:40180184-40180203 None:intergenic 40.0%
! TTCCATCTGAGTTGACTACT+TGG + Chr2:40180881-40180900 MsG0280008863.01.T01:exon 40.0%
!! TGATGGTGTTACTAACACAG+TGG + Chr2:40178668-40178687 MsG0280008863.01.T01:intron 40.0%
!!! ACACCATCAAATGGTTTTGG+TGG - Chr2:40178657-40178676 None:intergenic 40.0%
AAGGAAGTAATGGCAAAGGG+AGG - Chr2:40178865-40178884 None:intergenic 45.0%
AAGTGCGGCGATTTAGTTGA+TGG - Chr2:40179035-40179054 None:intergenic 45.0%
AAGTGGACAAGGGGTGAAAA+TGG - Chr2:40179969-40179988 None:intergenic 45.0%
AATATGGAAGGTCCATGCTC+AGG - Chr2:40180478-40180497 None:intergenic 45.0%
ACACCTCTGCATGTTCTCAA+AGG - Chr2:40180222-40180241 None:intergenic 45.0%
AGGAAGTAATGGCAAAGGGA+GGG - Chr2:40178864-40178883 None:intergenic 45.0%
AGGCAGAAACAACAGAATGG+GGG - Chr2:40179850-40179869 None:intergenic 45.0%
AGTGGACAAGGGGTGAAAAT+GGG - Chr2:40179968-40179987 None:intergenic 45.0%
ATACCAGCAGCAAAGGAAGA+TGG + Chr2:40178579-40178598 MsG0280008863.01.T01:intron 45.0%
ATCAACTAAATCGCCGCACT+TGG + Chr2:40179034-40179053 MsG0280008863.01.T01:intron 45.0%
ATGGTTCACATCGAGAGTCT+TGG + Chr2:40178809-40178828 MsG0280008863.01.T01:intron 45.0%
CAAGAGGAAGTAAAGAGGGT+AGG - Chr2:40179900-40179919 None:intergenic 45.0%
CCTCCACCAAAACCATTTGA+TGG + Chr2:40178651-40178670 MsG0280008863.01.T01:intron 45.0%
GAGAGGCAGAAACAACAGAA+TGG - Chr2:40179853-40179872 None:intergenic 45.0%
GAGGCAGAAACAACAGAATG+GGG - Chr2:40179851-40179870 None:intergenic 45.0%
GCATATTCACTTTGGCACCA+AGG - Chr2:40180176-40180195 None:intergenic 45.0%
TACCAGCAGCAAAGGAAGAT+GGG + Chr2:40178580-40178599 MsG0280008863.01.T01:intron 45.0%
TCAACTAAATCGCCGCACTT+GGG + Chr2:40179035-40179054 MsG0280008863.01.T01:intron 45.0%
TGACCCAACATTTAGACTGC+GGG - Chr2:40180530-40180549 None:intergenic 45.0%
TGAGTGAGATACCTCTGTAG+CGG + Chr2:40179581-40179600 MsG0280008863.01.T01:intron 45.0%
TGGTTCACATCGAGAGTCTT+GGG + Chr2:40178810-40178829 MsG0280008863.01.T01:intron 45.0%
TGTTTCTCACATGCACTGTC+AGG + Chr2:40179928-40179947 MsG0280008863.01.T01:intron 45.0%
TTTAAGGAGTGGTATCGCTC+AGG + Chr2:40178737-40178756 MsG0280008863.01.T01:intron 45.0%
TTTGGAGGACTAGGTTCAAG+AGG + Chr2:40180790-40180809 MsG0280008863.01.T01:exon 45.0%
! CAGAGGTGTAATTGTTCACC+TGG + Chr2:40180233-40180252 MsG0280008863.01.T01:CDS 45.0%
! CTTCCTTTGAGAACATGCAG+AGG + Chr2:40180216-40180235 MsG0280008863.01.T01:CDS 45.0%
! GGTTGTATTTTCCGCTACAG+AGG - Chr2:40179595-40179614 None:intergenic 45.0%
! GTTTTCACTGGAGCAAAAGC+TGG + Chr2:40180667-40180686 MsG0280008863.01.T01:exon 45.0%
! TAGGGGTTCTAATGAAGAGG+TGG + Chr2:40179320-40179339 MsG0280008863.01.T01:intron 45.0%
! TGGTTGGCTAGGTAGTTTGA+TGG + Chr2:40180825-40180844 MsG0280008863.01.T01:exon 45.0%
! TTCAGGTGTTATCCTGAGCA+TGG + Chr2:40180463-40180482 MsG0280008863.01.T01:intron 45.0%
!! TGGAGCAAAAGCTGGTTTGA+GGG + Chr2:40180675-40180694 MsG0280008863.01.T01:exon 45.0%
!!! CCATCAAATGGTTTTGGTGG+AGG - Chr2:40178654-40178673 None:intergenic 45.0%
!!! TCCACTCCCTTTTTTCCATG+TGG + Chr2:40179824-40179843 MsG0280008863.01.T01:intron 45.0%
AAGGAGACAGTCGGCATTAG+TGG + Chr2:40179092-40179111 MsG0280008863.01.T01:intron 50.0%
ACATGGATCGCTTCCACTTC+TGG + Chr2:40180717-40180736 MsG0280008863.01.T01:exon 50.0%
AGAAGTGGAAGCGATCCATG+TGG - Chr2:40180718-40180737 None:intergenic 50.0%
AGGAGACAGTCGGCATTAGT+GGG + Chr2:40179093-40179112 MsG0280008863.01.T01:intron 50.0%
AGGAGAGGAACAAGTGGACA+AGG - Chr2:40179980-40179999 None:intergenic 50.0%
ATGGGGGCCACATGGAAAAA+AGG - Chr2:40179834-40179853 None:intergenic 50.0%
ATGTTGGGTCAACTTGGGAG+TGG + Chr2:40180539-40180558 MsG0280008863.01.T01:exon 50.0%
CATGGATCGCTTCCACTTCT+GGG + Chr2:40180718-40180737 MsG0280008863.01.T01:exon 50.0%
GAGAGGAACAAGTGGACAAG+GGG - Chr2:40179978-40179997 None:intergenic 50.0%
GCATCTAGCAAGGAGACAGT+CGG + Chr2:40179083-40179102 MsG0280008863.01.T01:intron 50.0%
GCCACATGGAAAAAAGGGAG+TGG - Chr2:40179828-40179847 None:intergenic 50.0%
GCTCCCGCAGTCTAAATGTT+GGG + Chr2:40180524-40180543 MsG0280008863.01.T01:exon 50.0%
GGAATCCTGTACTGAGAGAG+TGG + Chr2:40179692-40179711 MsG0280008863.01.T01:intron 50.0%
GGAGAGGAACAAGTGGACAA+GGG - Chr2:40179979-40179998 None:intergenic 50.0%
GGTATCGCTCAGGCAGAATT+TGG + Chr2:40178747-40178766 MsG0280008863.01.T01:intron 50.0%
GTATTGAGGGAAAGCTGAGC+AGG - Chr2:40179725-40179744 None:intergenic 50.0%
TCGTATAATCAGGCGGTTCC+AGG - Chr2:40180254-40180273 None:intergenic 50.0%
TCTTCATCAGCTACTGCCGA+AGG + Chr2:40180612-40180631 MsG0280008863.01.T01:exon 50.0%
TGGAACCGCCTGATTATACG+AGG + Chr2:40180253-40180272 MsG0280008863.01.T01:CDS 50.0%
TGGGGGCCACATGGAAAAAA+GGG - Chr2:40179833-40179852 None:intergenic 50.0%
! ACTGTCAGGCCCTTTTCAGA+TGG + Chr2:40179942-40179961 MsG0280008863.01.T01:intron 50.0%
! TGCTTCCTGAGATTTCTGGG+AGG + Chr2:40180193-40180212 MsG0280008863.01.T01:CDS 50.0%
!! CTGGAGCAAAAGCTGGTTTG+AGG + Chr2:40180674-40180693 MsG0280008863.01.T01:exon 50.0%
!! GGCATTGGTAGCATCTAGCA+AGG + Chr2:40179073-40179092 MsG0280008863.01.T01:intron 50.0%
ACAACAGAATGGGGGCCACA+TGG - Chr2:40179842-40179861 None:intergenic 55.0%
ACTTGGGCTTGTGTGTGGAG+AGG + Chr2:40179051-40179070 MsG0280008863.01.T01:intron 55.0%
CTTGGGCTTGTGTGTGGAGA+GGG + Chr2:40179052-40179071 MsG0280008863.01.T01:intron 55.0%
GGAAGCCTCCCAGAAATCTC+AGG - Chr2:40180201-40180220 None:intergenic 55.0%
GGAGACAGTCGGCATTAGTG+GGG + Chr2:40179094-40179113 MsG0280008863.01.T01:intron 55.0%
GGCTACCACTCTCTCAGTAC+AGG - Chr2:40179700-40179719 None:intergenic 55.0%
GGCTCCCGCAGTCTAAATGT+TGG + Chr2:40180523-40180542 MsG0280008863.01.T01:exon 55.0%
TCCACACACAAGCCCAAGTG+CGG - Chr2:40179050-40179069 None:intergenic 55.0%
TGAGGGAAAGCTGAGCAGGA+AGG - Chr2:40179721-40179740 None:intergenic 55.0%
! CTTGTGTGTGGAGAGGGCAT+TGG + Chr2:40179058-40179077 MsG0280008863.01.T01:intron 55.0%
AAGGATGGAGCTGTGGGTCC+TGG + Chr2:40178611-40178630 MsG0280008863.01.T01:intron 60.0%
AGCGATCCATGTGGCGTGAG+TGG - Chr2:40180709-40180728 None:intergenic 60.0%
ATGCCTCCACTCACGCCACA+TGG + Chr2:40180700-40180719 MsG0280008863.01.T01:exon 60.0%
GATCCATGTGGCGTGAGTGG+AGG - Chr2:40180706-40180725 None:intergenic 60.0%
GGCCCATCTTCCTTTGCTGC+TGG - Chr2:40178585-40178604 None:intergenic 60.0%
GCCGCACTTGGGCTTGTGTG+TGG + Chr2:40179046-40179065 MsG0280008863.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 40178576 40181091 40178576 ID=MsG0280008864.01;Name=MsG0280008864.01
Chr2 mRNA 40178576 40181091 40178576 ID=MsG0280008864.01.T01;Parent=MsG0280008864.01;Name=MsG0280008864.01.T01;_AED=0.40;_eAED=0.56;_QI=0|0.33|0.5|1|1|1|4|164|283
Chr2 exon 40178576 40178778 40178576 ID=MsG0280008864.01.T01:exon:12873;Parent=MsG0280008864.01.T01
Chr2 exon 40179518 40179579 40179518 ID=MsG0280008864.01.T01:exon:12874;Parent=MsG0280008864.01.T01
Chr2 exon 40180148 40180274 40180148 ID=MsG0280008864.01.T01:exon:12875;Parent=MsG0280008864.01.T01
Chr2 exon 40180468 40181091 40180468 ID=MsG0280008864.01.T01:exon:12876;Parent=MsG0280008864.01.T01
Chr2 CDS 40178576 40178778 40178576 ID=MsG0280008864.01.T01:cds;Parent=MsG0280008864.01.T01
Chr2 CDS 40179518 40179579 40179518 ID=MsG0280008864.01.T01:cds;Parent=MsG0280008864.01.T01
Chr2 CDS 40180148 40180274 40180148 ID=MsG0280008864.01.T01:cds;Parent=MsG0280008864.01.T01
Chr2 CDS 40180468 40180927 40180468 ID=MsG0280008864.01.T01:cds;Parent=MsG0280008864.01.T01
Chr2 three_prime_UTR 40180928 40181091 40180928 ID=MsG0280008864.01.T01:three_prime_utr;Parent=MsG0280008864.01.T01
Gene Sequence

>MsG0280008864.01.T01

ATGATACCAGCAGCAAAGGAAGATGGGCCTTTATTAAGGATGGAGCTGTGGGTCCTGGACATTATAACAAAGTTTCCTCCACCAAAACCATTTGATGGTGTTACTAACACAGTGGCTGCTGCCACGATGAAATTGCACAACCTTAAGCGTCGAAAACAATCTTTAAGGAGTGGTATCGCTCAGGCAGAATTTGGTAGAAACTGGACTCGAACAAGTAAGGGAAACTTGTATGTGTATTGTTGTGGAAAAAGAAATCTGATCAGAGGTGAATATGATGCAGTCATTATATGCCTTGGTGCCAAAGTGAATATGCTTCCTGAGATTTCTGGGAGGCTTCCTTTGAGAACATGCAGAGGTGTAATTGTTCACCTGGAACCGCCTGATTATACGAGGTGTTATCCTGAGCATGGACCTTCCATATTATCAGATGCATGGATTTCTGTTCAGGGCTCCCGCAGTCTAAATGTTGGGTCAACTTGGGAGTGGAAATCTATAAATTCGTCGTCAAATGTTTCTACTGATGAAGCTTCAAAAGCTCTTCATCAGCTACTGCCGAAGGCATCAGTCATTTATCCCGGAATAAAGGATTGGGTTTTCACTGGAGCAAAAGCTGGTTTGAGGGCAATGCCTCCACTCACGCCACATGGATCGCTTCCACTTCTGGGTTGTATTAACGATTTCATTGGCAGAAACCATAGTTGTAAGTATTGGTTATTTGGAGGACTAGGTTCAAGAGGATTGTTATATCATGGTTGGCTAGGTAGTTTGATGGCACATGCTGTACTTTCCTGTAATGAAGAAGTAATTCCATCTGAGTTGACTACTTGGAAAAACATTAAATCCAAACTTTAG
Protein sequence

>MsG0280008864.01.T01

MIPAAKEDGPLLRMELWVLDIITKFPPPKPFDGVTNTVAAATMKLHNLKRRKQSLRSGIAQAEFGRNWTRTSKGNLYVYCCGKRNLIRGEYDAVIICLGAKVNMLPEISGRLPLRTCRGVIVHLEPPDYTRCYPEHGPSILSDAWISVQGSRSLNVGSTWEWKSINSSSNVSTDEASKALHQLLPKASVIYPGIKDWVFTGAKAGLRAMPPLTPHGSLPLLGCINDFIGRNHSCKYWLFGGLGSRGLLYHGWLGSLMAHAVLSCNEEVIPSELTTWKNIKSKL*