AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009749.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009749.01.T01 MTR_2g064890 82.906 117 2 1 1 99 1 117 1.39e-63 188
MsG0280009749.01.T01 MTR_5g041790 56.818 88 17 2 17 83 24 111 1.47e-26 95.9
MsG0280009749.01.T01 MTR_5g041790 65.574 61 18 1 17 74 24 84 1.25e-21 82.0
MsG0280009749.01.T01 MTR_2g034750 84.375 32 5 0 36 67 89 120 6.84e-14 63.5
MsG0280009749.01.T01 MTR_2g034750 84.375 32 5 0 36 67 71 102 9.72e-14 62.8
MsG0280009749.01.T01 MTR_2g034750 84.375 32 5 0 36 67 89 120 1.24e-13 62.8
MsG0280009749.01.T01 MTR_2g034750 84.375 32 5 0 36 67 87 118 1.24e-13 62.8
MsG0280009749.01.T01 MTR_1g025530 54.688 64 16 3 32 85 65 125 2.59e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009749.01.T01 AT5G64130 53.390 118 32 3 1 98 1 115 1.36e-33 112
MsG0280009749.01.T01 AT5G64130 44.056 143 32 4 1 98 1 140 4.90e-28 99.4
MsG0280009749.01.T01 AT1G69510 53.247 77 16 3 28 85 24 99 1.10e-17 72.8
MsG0280009749.01.T01 AT1G69510 53.247 77 16 3 28 85 24 99 1.10e-17 72.8
MsG0280009749.01.T01 AT1G69510 53.247 77 16 3 28 85 24 99 1.10e-17 72.8
MsG0280009749.01.T01 AT4G16146 52.727 55 26 0 13 67 1 55 3.38e-13 60.5

Find 54 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGCGTAAGGGGATTTC+CGG 0.294868 2:-57508195 None:intergenic
ATATGAAAAGAACCTGCTTT+TGG 0.296974 2:-57506733 None:intergenic
TATTGTTCTGATACCTCATT+AGG 0.302021 2:+57508383 MsG0280009749.01.T01:three_prime_UTR
TAAAATACTGTTGATTTGTT+TGG 0.325835 2:+57508658 MsG0280009749.01.T01:three_prime_UTR
GATTCTGCGGATTGGGCATT+GGG 0.345951 2:+57507119 MsG0280009749.01.T01:CDS
GTATGAAGGGAAGGGGAAGA+AGG 0.367540 2:-57508529 None:intergenic
CGATTCTGCGGATTGGGCAT+TGG 0.369174 2:+57507118 MsG0280009749.01.T01:CDS
ACTGTTGATTTGTTTGGTTT+CGG 0.372206 2:+57508664 MsG0280009749.01.T01:three_prime_UTR
TTACTTCGATTCTGCGGATT+GGG 0.392065 2:+57507112 MsG0280009749.01.T01:CDS
CTTACTTCGATTCTGCGGAT+TGG 0.402391 2:+57507111 MsG0280009749.01.T01:CDS
CGCAGCAGCAAACACGTTAC+CGG 0.407404 2:+57508176 MsG0280009749.01.T01:CDS
CTCAACACCGACCGGACGTT+CGG 0.417595 2:+57503829 MsG0280009749.01.T01:five_prime_UTR
AAATCCCCTTACGCTCCTTC+CGG 0.426971 2:+57508199 MsG0280009749.01.T01:CDS
TTCAAAACTATGAAATAAAG+AGG 0.443750 2:-57508551 None:intergenic
TGCTGCTTCCCTCACATCTC+CGG 0.445219 2:+57503870 MsG0280009749.01.T01:five_prime_UTR
TATGAAATAAAGAGGTATGA+AGG 0.473794 2:-57508543 None:intergenic
TGAGCGGAGCACCGAACGTC+CGG 0.474962 2:-57503840 None:intergenic
TATGAAGGGAAGGGGAAGAA+GGG 0.481069 2:-57508528 None:intergenic
TAACGTGTTTGCTGCTGCGT+TGG 0.485967 2:-57508172 None:intergenic
AAACAATAAGAAATTAATGA+TGG 0.496359 2:+57508582 MsG0280009749.01.T01:three_prime_UTR
TAAAGAGGTATGAAGGGAAG+GGG 0.508738 2:-57508536 None:intergenic
CCTTCACCGGAAGGAGCGTA+AGG 0.513456 2:-57508205 None:intergenic
CCTTACGCTCCTTCCGGTGA+AGG 0.513735 2:+57508205 MsG0280009749.01.T01:CDS
AAATTAAAACATACCTTCAC+CGG 0.515718 2:-57508218 None:intergenic
TGCGGATTGGGCATTGGGAA+AGG 0.521134 2:+57507124 MsG0280009749.01.T01:CDS
TTGGAAGCTGCATCCTCAGA+TGG 0.522717 2:-57508341 None:intergenic
AAACAAAGACATTGGACAGA+TGG 0.536462 2:+57506670 MsG0280009749.01.T01:CDS
ATGAAATAAAGAGGTATGAA+GGG 0.536689 2:-57508542 None:intergenic
AAGAAATTAATGATGGATAG+TGG 0.544714 2:+57508589 MsG0280009749.01.T01:three_prime_UTR
ATAAAGAGGTATGAAGGGAA+GGG 0.546301 2:-57508537 None:intergenic
GTTCGGTGCTCCGCTCATCA+CGG 0.546326 2:+57503846 MsG0280009749.01.T01:five_prime_UTR
AATAAAGAGGTATGAAGGGA+AGG 0.554646 2:-57508538 None:intergenic
CATGCCATCACCCCAAAAGC+AGG 0.559564 2:+57506721 MsG0280009749.01.T01:CDS
ATAGAGGAAAACAAAGACAT+TGG 0.560634 2:+57506662 MsG0280009749.01.T01:CDS
AAAGCCACCACTTATTTCCA+AGG 0.561400 2:+57506924 MsG0280009749.01.T01:CDS
GAGCTCAACTCAACACCGAC+CGG 0.573706 2:+57503821 MsG0280009749.01.T01:five_prime_UTR
ATGGATGTGGATGCATCTGA+TGG 0.574994 2:+57506689 MsG0280009749.01.T01:CDS
AATCGAAGTAAGCACGCTCA+TGG 0.583023 2:-57507100 None:intergenic
GGATCACATGTCAAACATAG+AGG 0.587961 2:+57506646 MsG0280009749.01.T01:exon
AGAACTGACCGGAGATGTGA+GGG 0.588935 2:-57503878 None:intergenic
AGGTGGAAGTGTTCCATCTG+AGG 0.593464 2:+57508328 MsG0280009749.01.T01:CDS
TTCACCGGAAGGAGCGTAAG+GGG 0.603501 2:-57508203 None:intergenic
AAGAACTGACCGGAGATGTG+AGG 0.611778 2:-57503879 None:intergenic
CTTCACCGGAAGGAGCGTAA+GGG 0.612254 2:-57508204 None:intergenic
AGTCCCTACAGCAAAACTGC+AGG 0.614184 2:-57508480 None:intergenic
TTAATGATGGATAGTGGTCA+TGG 0.625530 2:+57508595 MsG0280009749.01.T01:three_prime_UTR
TAAAACATACCTTCACCGGA+AGG 0.628441 2:-57508214 None:intergenic
GAAGCAGCAACCGTGATGAG+CGG 0.632408 2:-57503856 None:intergenic
TGGTGTACCTTGGAAATAAG+TGG 0.633332 2:-57506931 None:intergenic
AGACATTGGACAGATGGATG+TGG 0.636895 2:+57506676 MsG0280009749.01.T01:CDS
GCGTGCTTACTTCGATTCTG+CGG 0.644582 2:+57507106 MsG0280009749.01.T01:CDS
CGGAGCACCGAACGTCCGGT+CGG 0.644924 2:-57503836 None:intergenic
TGGATGTGGATGCATCTGAT+GGG 0.677419 2:+57506690 MsG0280009749.01.T01:CDS
TGTACCTTGGAAATAAGTGG+TGG 0.738918 2:-57506928 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAGAAATTCATT+TGG - Chr2:57506837-57506856 None:intergenic 10.0%
!! AAACAATAAGAAATTAATGA+TGG + Chr2:57508582-57508601 MsG0280009749.01.T01:three_prime_UTR 15.0%
!! AATTAGTATTAAGTATTAAG+TGG + Chr2:57504990-57505009 MsG0280009749.01.T01:intron 15.0%
!! AATTGTAAATTTGCAATATT+AGG + Chr2:57504282-57504301 MsG0280009749.01.T01:intron 15.0%
!!! ACAAATAAATTTTCTAATTG+TGG + Chr2:57507668-57507687 MsG0280009749.01.T01:intron 15.0%
!!! AGAAAAAGAAATAAAAGTAT+TGG - Chr2:57506083-57506102 None:intergenic 15.0%
!!! ATTTCTTTTTGTTGAAATTT+GGG + Chr2:57505748-57505767 MsG0280009749.01.T01:intron 15.0%
!!! GAAAAAGAAATAAAAGTATT+GGG - Chr2:57506082-57506101 None:intergenic 15.0%
!!! GAAACTTTAAAGAAAAAATA+TGG + Chr2:57506886-57506905 MsG0280009749.01.T01:CDS 15.0%
!!! TAAATTCTTCTTTTTATAGA+AGG + Chr2:57508308-57508327 MsG0280009749.01.T01:intron 15.0%
!!! TTTATTTCATAGTTTTGAAA+AGG + Chr2:57508555-57508574 MsG0280009749.01.T01:three_prime_UTR 15.0%
!! AAATTAAATACCTGTAGTTT+AGG - Chr2:57507479-57507498 None:intergenic 20.0%
!! AATGAATTAATTGAACGTAA+CGG - Chr2:57504143-57504162 None:intergenic 20.0%
!! ACTTCATCTGAAAAATTTAT+TGG - Chr2:57506197-57506216 None:intergenic 20.0%
!! ATTAGATATTCTCTTTACTA+TGG + Chr2:57506800-57506819 MsG0280009749.01.T01:intron 20.0%
!! CAAAATAATTGAAGCTAAAT+CGG - Chr2:57503959-57503978 None:intergenic 20.0%
!! CCATATATAAATCAGATATT+GGG - Chr2:57507511-57507530 None:intergenic 20.0%
!! CTTCATCTGAAAAATTTATT+GGG - Chr2:57506196-57506215 None:intergenic 20.0%
!! TATAAAACAAGAATAGAGAT+CGG - Chr2:57507799-57507818 None:intergenic 20.0%
!! TCAAGCACATTAAAATAAAA+TGG - Chr2:57508122-57508141 None:intergenic 20.0%
!! TGCAAATTTACAATTAAACA+AGG - Chr2:57504277-57504296 None:intergenic 20.0%
!! TGTAATAATAAATACTCCAT+CGG - Chr2:57507845-57507864 None:intergenic 20.0%
!! TTCAAAACTATGAAATAAAG+AGG - Chr2:57508554-57508573 None:intergenic 20.0%
!! TTTCTATTTGTTTAAATGTG+TGG + Chr2:57506363-57506382 MsG0280009749.01.T01:intron 20.0%
!!! AATTTTTTAATAACGTATCG+AGG + Chr2:57505175-57505194 MsG0280009749.01.T01:intron 20.0%
!!! ACTTTAAAGAAAAAATATGG+AGG + Chr2:57506889-57506908 MsG0280009749.01.T01:CDS 20.0%
!!! GATTTCTTTTTGTTGAAATT+TGG + Chr2:57505747-57505766 MsG0280009749.01.T01:intron 20.0%
!!! TAAAATACTGTTGATTTGTT+TGG + Chr2:57508658-57508677 MsG0280009749.01.T01:three_prime_UTR 20.0%
!!! TTTAGCTTCAATTATTTTGT+TGG + Chr2:57503960-57503979 MsG0280009749.01.T01:intron 20.0%
!!! TTTCTTTTTGTTGAAATTTG+GGG + Chr2:57505749-57505768 MsG0280009749.01.T01:intron 20.0%
!!! TTTTAACCCCAAAAAAATTA+GGG + Chr2:57504723-57504742 MsG0280009749.01.T01:intron 20.0%
!!! TTTTTTTAAATTATTACCGC+CGG + Chr2:57504163-57504182 MsG0280009749.01.T01:intron 20.0%
! AAAAAATTACCAATACGTGT+CGG + Chr2:57504575-57504594 MsG0280009749.01.T01:intron 25.0%
! AAATTAAAACATACCTTCAC+CGG - Chr2:57508221-57508240 None:intergenic 25.0%
! ACAAAAAGTTATACCTAATG+AGG - Chr2:57508399-57508418 None:intergenic 25.0%
! ACTATCCTAATCAAAAGTAA+AGG - Chr2:57505428-57505447 None:intergenic 25.0%
! CCCAATATCTGATTTATATA+TGG + Chr2:57507508-57507527 MsG0280009749.01.T01:intron 25.0%
! CTAATATGTCAAGAACTAAT+TGG - Chr2:57504229-57504248 None:intergenic 25.0%
! CTGATACTATGTTGAAATTT+GGG + Chr2:57505953-57505972 MsG0280009749.01.T01:intron 25.0%
! GCACATTAAAATAAAATGGA+AGG - Chr2:57508118-57508137 None:intergenic 25.0%
! GCCATATATAAATCAGATAT+TGG - Chr2:57507512-57507531 None:intergenic 25.0%
! TATGAGTATGATCATGTTAA+AGG - Chr2:57508095-57508114 None:intergenic 25.0%
! TCATAGTTGAACACAATTAA+GGG + Chr2:57507888-57507907 MsG0280009749.01.T01:intron 25.0%
! TCATGGTGTAAAAATAGAAT+AGG + Chr2:57508612-57508631 MsG0280009749.01.T01:three_prime_UTR 25.0%
! TCTGATACTATGTTGAAATT+TGG + Chr2:57505952-57505971 MsG0280009749.01.T01:intron 25.0%
! TGATACTATGTTGAAATTTG+GGG + Chr2:57505954-57505973 MsG0280009749.01.T01:intron 25.0%
! TTAATTGTGTTCAACTATGA+AGG - Chr2:57507888-57507907 None:intergenic 25.0%
! TTCATAGTTGAACACAATTA+AGG + Chr2:57507887-57507906 MsG0280009749.01.T01:intron 25.0%
!! AAAGTCTGTAATCAGAATTT+TGG - Chr2:57506248-57506267 None:intergenic 25.0%
!! AAGAAAAACTTCAAGTTAGA+AGG + Chr2:57505633-57505652 MsG0280009749.01.T01:intron 25.0%
!! AATATGTACGAAAGAAGTAT+CGG - Chr2:57504625-57504644 None:intergenic 25.0%
!! AGAAAAACTTCAAGTTAGAA+GGG + Chr2:57505634-57505653 MsG0280009749.01.T01:intron 25.0%
!! ATGAAATAAAGAGGTATGAA+GGG - Chr2:57508545-57508564 None:intergenic 25.0%
!! ATTCTTCTTTTTATAGAAGG+TGG + Chr2:57508311-57508330 MsG0280009749.01.T01:intron 25.0%
!! CTTTTAACCCCAAAAAAATT+AGG + Chr2:57504722-57504741 MsG0280009749.01.T01:intron 25.0%
!! TATGAAATAAAGAGGTATGA+AGG - Chr2:57508546-57508565 None:intergenic 25.0%
!!! AAAGAGAAGAAGGTATTAAA+GGG - Chr2:57504907-57504926 None:intergenic 25.0%
!!! AAATTTTGAGTAGTCTCAAT+CGG + Chr2:57505595-57505614 MsG0280009749.01.T01:intron 25.0%
!!! AAGAAATTAATGATGGATAG+TGG + Chr2:57508589-57508608 MsG0280009749.01.T01:three_prime_UTR 25.0%
!!! AATGTCCTTTACTTTTGATT+AGG + Chr2:57505420-57505439 MsG0280009749.01.T01:intron 25.0%
!!! CGAGAATTTTGAAAATTTTC+TGG + Chr2:57505209-57505228 MsG0280009749.01.T01:intron 25.0%
!!! GAAGGTATGTTTTAATTTGA+TGG + Chr2:57508223-57508242 MsG0280009749.01.T01:intron 25.0%
!!! TAAAGAGAAGAAGGTATTAA+AGG - Chr2:57504908-57504927 None:intergenic 25.0%
!!! TCTACAAAACAACTAGTTTT+CGG - Chr2:57508001-57508020 None:intergenic 25.0%
!!! TCTCCATTTCTTTTGAAAAA+CGG - Chr2:57507736-57507755 None:intergenic 25.0%
!!! TTCTTGTTTTATAATCCAGT+TGG + Chr2:57507806-57507825 MsG0280009749.01.T01:intron 25.0%
AAGAACAGATAAAGAGAAGA+AGG - Chr2:57504917-57504936 None:intergenic 30.0%
AATAATAAATACTCCATCGG+TGG - Chr2:57507842-57507861 None:intergenic 30.0%
ATAGAGGAAAACAAAGACAT+TGG + Chr2:57506662-57506681 MsG0280009749.01.T01:CDS 30.0%
ATCTGTTCTTCTCTCTAATA+AGG + Chr2:57504927-57504946 MsG0280009749.01.T01:intron 30.0%
CGAAGTTTATGAAATCAAGA+CGG + Chr2:57506996-57507015 MsG0280009749.01.T01:intron 30.0%
CTTGAGAAGAGTTTATAAAG+AGG - Chr2:57505820-57505839 None:intergenic 30.0%
GCGTGATTACATTTAAATGA+CGG + Chr2:57506110-57506129 MsG0280009749.01.T01:intron 30.0%
TACCAAATGCTGTCAAATAA+CGG + Chr2:57507289-57507308 MsG0280009749.01.T01:intron 30.0%
TATTGTTCTGATACCTCATT+AGG + Chr2:57508383-57508402 MsG0280009749.01.T01:three_prime_UTR 30.0%
TCAAAATTCTCGATACGATA+CGG - Chr2:57505201-57505220 None:intergenic 30.0%
TGAGACTACTCAAAATTTAG+TGG - Chr2:57505593-57505612 None:intergenic 30.0%
TTAGCAGAAAATTCTCAAGT+AGG - Chr2:57506586-57506605 None:intergenic 30.0%
TTCTAACTGATATAGTTGCA+AGG + Chr2:57506280-57506299 MsG0280009749.01.T01:intron 30.0%
! AGCTATGTTAGCTTATCTAT+GGG + Chr2:57507636-57507655 MsG0280009749.01.T01:intron 30.0%
! ATATGAAAAGAACCTGCTTT+TGG - Chr2:57506736-57506755 None:intergenic 30.0%
! TAGCTATGTTAGCTTATCTA+TGG + Chr2:57507635-57507654 MsG0280009749.01.T01:intron 30.0%
! TGACATTTAAATTTTGTGCC+AGG + Chr2:57507076-57507095 MsG0280009749.01.T01:intron 30.0%
! TTGAGAATTTTCTGCTAACA+TGG + Chr2:57506588-57506607 MsG0280009749.01.T01:intron 30.0%
! TTGATAATAACTGTTCTGCT+GGG + Chr2:57507552-57507571 MsG0280009749.01.T01:intron 30.0%
!! AATTTTGAGTAGTCTCAATC+GGG + Chr2:57505596-57505615 MsG0280009749.01.T01:intron 30.0%
!! ACTGTTGATTTGTTTGGTTT+CGG + Chr2:57508664-57508683 MsG0280009749.01.T01:three_prime_UTR 30.0%
!! GAAAACTAGTTGTTTTGTAG+AGG + Chr2:57508000-57508019 MsG0280009749.01.T01:intron 30.0%
!! TAGGTGCTATTTAACAAGAT+TGG - Chr2:57507206-57507225 None:intergenic 30.0%
!! TATGAAAAGAACCTGCTTTT+GGG - Chr2:57506735-57506754 None:intergenic 30.0%
!! TCTCCGTTTTTCAAAAGAAA+TGG + Chr2:57507730-57507749 MsG0280009749.01.T01:intron 30.0%
!! TGATTTGTTCAAATTCCACT+AGG - Chr2:57507243-57507262 None:intergenic 30.0%
!!! AATCAGAATTTTGGTTTGCA+GGG - Chr2:57506239-57506258 None:intergenic 30.0%
!!! ATATTGCTGCTTTTACTCAT+TGG - Chr2:57508363-57508382 None:intergenic 30.0%
!!! TAATCAGAATTTTGGTTTGC+AGG - Chr2:57506240-57506259 None:intergenic 30.0%
!!! TTTCACCATTTTTGGAGTAT+CGG - Chr2:57504464-57504483 None:intergenic 30.0%
AAACAAAGACATTGGACAGA+TGG + Chr2:57506670-57506689 MsG0280009749.01.T01:CDS 35.0%
AAATTCTCAAGTAGGTAGCT+TGG - Chr2:57506578-57506597 None:intergenic 35.0%
AACAAATAGAAAGACCAGCA+TGG - Chr2:57506355-57506374 None:intergenic 35.0%
AACCGTTATTTGACAGCATT+TGG - Chr2:57507294-57507313 None:intergenic 35.0%
ATAGTAATGCTATAGCCTAG+TGG + Chr2:57507225-57507244 MsG0280009749.01.T01:intron 35.0%
ATCAGAGCTTGTCAAATTCA+AGG - Chr2:57505938-57505957 None:intergenic 35.0%
ATCTATCCAATGTGGGAATT+GGG + Chr2:57506051-57506070 MsG0280009749.01.T01:intron 35.0%
ATGCTGAGAAACTGTAGTAA+TGG - Chr2:57508271-57508290 None:intergenic 35.0%
ATTGGTATCAGATACTTGTC+TGG - Chr2:57504494-57504513 None:intergenic 35.0%
CACGAGTTTATAAACCTTGT+AGG + Chr2:57506861-57506880 MsG0280009749.01.T01:intron 35.0%
CTAACTCATCCTTACAAAAC+CGG + Chr2:57505773-57505792 MsG0280009749.01.T01:intron 35.0%
CTAGGCTATAGCATTACTAT+AGG - Chr2:57507225-57507244 None:intergenic 35.0%
CTGCATACATTGAGATCAAT+TGG - Chr2:57506163-57506182 None:intergenic 35.0%
GACACATGAAGATTGAGAAA+AGG - Chr2:57503930-57503949 None:intergenic 35.0%
GAGAAGAGTTTATAAAGAGG+AGG - Chr2:57505817-57505836 None:intergenic 35.0%
GAGTTTATAAACCTTGTAGG+AGG + Chr2:57506864-57506883 MsG0280009749.01.T01:intron 35.0%
GGCTAAAAAAGAAGAATGTG+TGG - Chr2:57504256-57504275 None:intergenic 35.0%
TATCTATCCAATGTGGGAAT+TGG + Chr2:57506050-57506069 MsG0280009749.01.T01:intron 35.0%
TCAGAGCTTGTCAAATTCAA+GGG - Chr2:57505937-57505956 None:intergenic 35.0%
TCTACTAGAATATGCTTGAC+AGG + Chr2:57505297-57505316 MsG0280009749.01.T01:intron 35.0%
TGATCTACTAAGTCTATGTC+TGG - Chr2:57505355-57505374 None:intergenic 35.0%
TGATGTAAAACTTCGCGTAA+AGG + Chr2:57504385-57504404 MsG0280009749.01.T01:intron 35.0%
TGCTGAGAAACTGTAGTAAT+GGG - Chr2:57508270-57508289 None:intergenic 35.0%
TTAGTAGATCACTTGTAAGC+TGG + Chr2:57505364-57505383 MsG0280009749.01.T01:intron 35.0%
TTATCGTATCGTATCCGTAT+CGG + Chr2:57505237-57505256 MsG0280009749.01.T01:intron 35.0%
TTGTGTTCAACTATGAAGGT+TGG - Chr2:57507884-57507903 None:intergenic 35.0%
TTTGTTTGAGAATCCAGTGT+GGG - Chr2:57505691-57505710 None:intergenic 35.0%
! AATAAAGAGGTATGAAGGGA+AGG - Chr2:57508541-57508560 None:intergenic 35.0%
! AGAAACTGTAGTAATGGGAA+GGG - Chr2:57508265-57508284 None:intergenic 35.0%
! AGAGTCTTATCTATCCAATG+TGG + Chr2:57506043-57506062 MsG0280009749.01.T01:intron 35.0%
! ATAAAGAGGTATGAAGGGAA+GGG - Chr2:57508540-57508559 None:intergenic 35.0%
! CTTGATAATAACTGTTCTGC+TGG + Chr2:57507551-57507570 MsG0280009749.01.T01:intron 35.0%
! GAAATAAGTGGTGGCTTTTT+GGG - Chr2:57506922-57506941 None:intergenic 35.0%
! GAGTCTTATCTATCCAATGT+GGG + Chr2:57506044-57506063 MsG0280009749.01.T01:intron 35.0%
! GATATAGTTGCAAGGTGTTT+AGG + Chr2:57506288-57506307 MsG0280009749.01.T01:intron 35.0%
! GATCATGTTAAAGGCACATT+TGG - Chr2:57508086-57508105 None:intergenic 35.0%
! GCACGATTTTTCACCATTTT+TGG - Chr2:57504472-57504491 None:intergenic 35.0%
! TCGACGCAAACTATTTTACT+AGG - Chr2:57507707-57507726 None:intergenic 35.0%
! TGCAGTATTTTACGCTCTAA+AGG + Chr2:57506625-57506644 MsG0280009749.01.T01:intron 35.0%
! TTTGAGGTAGCATTGTTCAT+TGG + Chr2:57505397-57505416 MsG0280009749.01.T01:intron 35.0%
!! ATGAAAAGAACCTGCTTTTG+GGG - Chr2:57506734-57506753 None:intergenic 35.0%
!! CTTTAAAGTTTCCTCCTACA+AGG - Chr2:57506878-57506897 None:intergenic 35.0%
!! TTAATGATGGATAGTGGTCA+TGG + Chr2:57508595-57508614 MsG0280009749.01.T01:three_prime_UTR 35.0%
!! TTTTGTTTGAGAATCCAGTG+TGG - Chr2:57505692-57505711 None:intergenic 35.0%
!!! ACGAGGAACCCTAATTTTTT+TGG - Chr2:57504734-57504753 None:intergenic 35.0%
!!! CGAGGAACCCTAATTTTTTT+GGG - Chr2:57504733-57504752 None:intergenic 35.0%
!!! GAGGAACCCTAATTTTTTTG+GGG - Chr2:57504732-57504751 None:intergenic 35.0%
!!! TTCACCATTTTTGGAGTATC+GGG - Chr2:57504463-57504482 None:intergenic 35.0%
AAAGCCACCACTTATTTCCA+AGG + Chr2:57506924-57506943 MsG0280009749.01.T01:CDS 40.0%
AATACTGCAGATGCAACAAC+AGG - Chr2:57506614-57506633 None:intergenic 40.0%
AGTGATGCTGATGTTGAGAT+TGG - Chr2:57504807-57504826 None:intergenic 40.0%
ATCTATCCGATGTGGGAATT+GGG + Chr2:57505846-57505865 MsG0280009749.01.T01:intron 40.0%
CAAGTGCAGATCATGGAAAA+TGG - Chr2:57507363-57507382 None:intergenic 40.0%
CGTTCTGCTTATATCTCCTT+TGG + Chr2:57506535-57506554 MsG0280009749.01.T01:intron 40.0%
CTGTCAAATAACGGTTGTAG+CGG + Chr2:57507298-57507317 MsG0280009749.01.T01:intron 40.0%
GAAAAACCCAATTCCCACAT+CGG - Chr2:57505855-57505874 None:intergenic 40.0%
GAAAAACCCAATTCCCACAT+TGG - Chr2:57505855-57505874 None:intergenic 40.0%
GAAGAAGAAGAAGAACTGAC+CGG - Chr2:57503892-57503911 None:intergenic 40.0%
GATGTTGAGATTGGGTCAAT+CGG - Chr2:57504798-57504817 None:intergenic 40.0%
GCAATGCACACGTGAATATT+TGG + Chr2:57507024-57507043 MsG0280009749.01.T01:intron 40.0%
GCTATATCACTTTCGCATAG+AGG + Chr2:57507322-57507341 MsG0280009749.01.T01:intron 40.0%
GGATCACATGTCAAACATAG+AGG + Chr2:57506646-57506665 MsG0280009749.01.T01:exon 40.0%
GTATTGTCAAGTGCAGATCA+TGG - Chr2:57507370-57507389 None:intergenic 40.0%
GTCAAAACTCAAGAACCATC+AGG - Chr2:57507592-57507611 None:intergenic 40.0%
GTGAATTGCTTATTGCCTGA+TGG + Chr2:57507574-57507593 MsG0280009749.01.T01:intron 40.0%
GTGATGCTGATGTTGAGATT+GGG - Chr2:57504806-57504825 None:intergenic 40.0%
TAAAACATACCTTCACCGGA+AGG - Chr2:57508217-57508236 None:intergenic 40.0%
TATCCTCATGTGAAACAGCA+AGG + Chr2:57507416-57507435 MsG0280009749.01.T01:intron 40.0%
TATCGTATCGTATCCGTATC+GGG + Chr2:57505238-57505257 MsG0280009749.01.T01:intron 40.0%
TATCTATCCGATGTGGGAAT+TGG + Chr2:57505845-57505864 MsG0280009749.01.T01:intron 40.0%
TCCGTTACGCAGAGAAATAT+CGG + Chr2:57504528-57504547 MsG0280009749.01.T01:intron 40.0%
TGGTGTACCTTGGAAATAAG+TGG - Chr2:57506934-57506953 None:intergenic 40.0%
TGTACCTTGGAAATAAGTGG+TGG - Chr2:57506931-57506950 None:intergenic 40.0%
TTACTTCGATTCTGCGGATT+GGG + Chr2:57507112-57507131 MsG0280009749.01.T01:CDS 40.0%
TTGGTTATGAAGCAGTGAGA+TGG - Chr2:57504210-57504229 None:intergenic 40.0%
! AAACTGTAGTAATGGGAAGG+GGG - Chr2:57508263-57508282 None:intergenic 40.0%
! ACTTCAAGTTAGAAGGGCAT+TGG + Chr2:57505640-57505659 MsG0280009749.01.T01:intron 40.0%
! AGAGTCTTATCTATCCGATG+TGG + Chr2:57505838-57505857 MsG0280009749.01.T01:intron 40.0%
! GAAACTGTAGTAATGGGAAG+GGG - Chr2:57508264-57508283 None:intergenic 40.0%
! GAGAAACTGTAGTAATGGGA+AGG - Chr2:57508266-57508285 None:intergenic 40.0%
! GAGTCTTATCTATCCGATGT+GGG + Chr2:57505839-57505858 MsG0280009749.01.T01:intron 40.0%
! GGAAATAAGTGGTGGCTTTT+TGG - Chr2:57506923-57506942 None:intergenic 40.0%
! TAAAGAGGTATGAAGGGAAG+GGG - Chr2:57508539-57508558 None:intergenic 40.0%
!! AACGTAACGGTTTTGATCAG+AGG - Chr2:57504130-57504149 None:intergenic 40.0%
!! CGGTGGTAATGGTTAGAAAA+GGG - Chr2:57504778-57504797 None:intergenic 40.0%
!! CGGTTTTGTAAGGATGAGTT+AGG - Chr2:57505775-57505794 None:intergenic 40.0%
!! TACGAAAGAAGTATCGGTGT+CGG - Chr2:57504619-57504638 None:intergenic 40.0%
!! TCGGTGGTAATGGTTAGAAA+AGG - Chr2:57504779-57504798 None:intergenic 40.0%
!!! TCACCATTTTTGGAGTATCG+GGG - Chr2:57504462-57504481 None:intergenic 40.0%
AAGCCCCGATACTCCAAAAA+TGG + Chr2:57504456-57504475 MsG0280009749.01.T01:intron 45.0%
AATCGAAGTAAGCACGCTCA+TGG - Chr2:57507103-57507122 None:intergenic 45.0%
AGAATCCAGTGTGGGCAAAT+GGG - Chr2:57505683-57505702 None:intergenic 45.0%
AGACATTGGACAGATGGATG+TGG + Chr2:57506676-57506695 MsG0280009749.01.T01:CDS 45.0%
AGCTGGATAGCAGTTCTTTG+AGG + Chr2:57505381-57505400 MsG0280009749.01.T01:intron 45.0%
AGGGAATAGCTATAGCTCAG+TGG - Chr2:57504759-57504778 None:intergenic 45.0%
ATCGTATCGTATCCGTATCG+GGG + Chr2:57505239-57505258 MsG0280009749.01.T01:intron 45.0%
ATGGATGTGGATGCATCTGA+TGG + Chr2:57506689-57506708 MsG0280009749.01.T01:CDS 45.0%
ATTGCCTCCATCAACAGCTT+CGG + Chr2:57504828-57504847 MsG0280009749.01.T01:intron 45.0%
ATTGGGTCAATCGGTGGTAA+TGG - Chr2:57504789-57504808 None:intergenic 45.0%
CAAGGTGGTAGTAAGCCTAA+GGG + Chr2:57507434-57507453 MsG0280009749.01.T01:intron 45.0%
CAGAAATCTGCATGCGTGTA+CGG - Chr2:57506957-57506976 None:intergenic 45.0%
CGGAACTTATCGGGACATAT+TGG - Chr2:57504512-57504531 None:intergenic 45.0%
CTTACTTCGATTCTGCGGAT+TGG + Chr2:57507111-57507130 MsG0280009749.01.T01:CDS 45.0%
GCCGATATTTCTCTGCGTAA+CGG - Chr2:57504532-57504551 None:intergenic 45.0%
GTGCAGATCATGGAAAATGG+CGG - Chr2:57507360-57507379 None:intergenic 45.0%
GTGCTTGACAAAGTTGTGCT+TGG + Chr2:57508134-57508153 MsG0280009749.01.T01:intron 45.0%
TAGCTGTATCCGACACGTAT+TGG - Chr2:57504587-57504606 None:intergenic 45.0%
TATGAAGGGAAGGGGAAGAA+GGG - Chr2:57508531-57508550 None:intergenic 45.0%
TATGTTTCTCATAGCGCTGC+AGG - Chr2:57504696-57504715 None:intergenic 45.0%
TATTTCCCATTTGCCCACAC+TGG + Chr2:57505675-57505694 MsG0280009749.01.T01:intron 45.0%
TGAAGCAGTGAGATGGACAT+CGG - Chr2:57504203-57504222 None:intergenic 45.0%
TGGATGTGGATGCATCTGAT+GGG + Chr2:57506690-57506709 MsG0280009749.01.T01:CDS 45.0%
TTCTCAAGTAGGTAGCTTGG+TGG - Chr2:57506575-57506594 None:intergenic 45.0%
TTGGTGCGTGAATATGTAGG+TGG + Chr2:57505911-57505930 MsG0280009749.01.T01:intron 45.0%
! CTCTGCGTAACGGAACTTAT+CGG - Chr2:57504522-57504541 None:intergenic 45.0%
! GTAGCTTGGTGGTAAGCTAA+GGG - Chr2:57506564-57506583 None:intergenic 45.0%
! TCTGCGTAACGGAACTTATC+GGG - Chr2:57504521-57504540 None:intergenic 45.0%
! TGGCAACGTTGCTTTCTTTG+TGG + Chr2:57507528-57507547 MsG0280009749.01.T01:intron 45.0%
! TGGTTTGCAGGGTAAAAGAG+AGG - Chr2:57506228-57506247 None:intergenic 45.0%
!! ACGGTTTTGATCAGAGGTGT+CGG - Chr2:57504124-57504143 None:intergenic 45.0%
!! CCTTACAAAACCGGCTTGTA+AGG + Chr2:57505782-57505801 MsG0280009749.01.T01:intron 45.0%
!! CCTTACAAGCCGGTTTTGTA+AGG - Chr2:57505785-57505804 None:intergenic 45.0%
!! GTTTGTTGTACTGTCCATGC+TGG + Chr2:57506338-57506357 MsG0280009749.01.T01:intron 45.0%
!! TGACCTGCAGTTTTGCTGTA+GGG + Chr2:57508477-57508496 MsG0280009749.01.T01:three_prime_UTR 45.0%
!! TTGACCTGCAGTTTTGCTGT+AGG + Chr2:57508476-57508495 MsG0280009749.01.T01:three_prime_UTR 45.0%
!! TCAAAAATATAAATATTAAT+TGG - Chr2:57504554-57504573 None:intergenic 5.0%
AAATCCCCTTACGCTCCTTC+CGG + Chr2:57508199-57508218 MsG0280009749.01.T01:CDS 50.0%
AAATCTGCATGCGTGTACGG+TGG - Chr2:57506954-57506973 None:intergenic 50.0%
AAGAACTGACCGGAGATGTG+AGG - Chr2:57503882-57503901 None:intergenic 50.0%
AGAACTGACCGGAGATGTGA+GGG - Chr2:57503881-57503900 None:intergenic 50.0%
AGTCCCTACAGCAAAACTGC+AGG - Chr2:57508483-57508502 None:intergenic 50.0%
CCTCATGTGAAACAGCAAGG+TGG + Chr2:57507419-57507438 MsG0280009749.01.T01:intron 50.0%
CTAGCTATGAAGCCCCGATA+CGG - Chr2:57505254-57505273 None:intergenic 50.0%
GAACTCTCCGAAGCTGTTGA+TGG - Chr2:57504838-57504857 None:intergenic 50.0%
GAGAATCCAGTGTGGGCAAA+TGG - Chr2:57505684-57505703 None:intergenic 50.0%
GCAAGGTGGTAGTAAGCCTA+AGG + Chr2:57507433-57507452 MsG0280009749.01.T01:intron 50.0%
GCAATCCTCACCTTACAAGC+CGG - Chr2:57505795-57505814 None:intergenic 50.0%
GCGTGCTTACTTCGATTCTG+CGG + Chr2:57507106-57507125 MsG0280009749.01.T01:CDS 50.0%
GGGGCTTCACAAATGACTAG+AGG - Chr2:57504443-57504462 None:intergenic 50.0%
GGTAAGCTAAGGGACACCAA+AGG - Chr2:57506554-57506573 None:intergenic 50.0%
GGTGGAAGCTTCAATCCAAC+TGG - Chr2:57507824-57507843 None:intergenic 50.0%
GTATGAAGGGAAGGGGAAGA+AGG - Chr2:57508532-57508551 None:intergenic 50.0%
GTTGAGATTGGGTCAATCGG+TGG - Chr2:57504795-57504814 None:intergenic 50.0%
TAAGCCTAAGGGACCACTTG+AGG + Chr2:57507445-57507464 MsG0280009749.01.T01:intron 50.0%
TGGATTGAAGCTTCCACCGA+TGG + Chr2:57507826-57507845 MsG0280009749.01.T01:intron 50.0%
TTGGAAGCTGCATCCTCAGA+TGG - Chr2:57508344-57508363 None:intergenic 50.0%
! AAGGAGCGTAAGGGGATTTC+CGG - Chr2:57508198-57508217 None:intergenic 50.0%
! GGTAGCTTGGTGGTAAGCTA+AGG - Chr2:57506565-57506584 None:intergenic 50.0%
! TAACGTGTTTGCTGCTGCGT+TGG - Chr2:57508175-57508194 None:intergenic 50.0%
!! AGGTGGAAGTGTTCCATCTG+AGG + Chr2:57508328-57508347 MsG0280009749.01.T01:CDS 50.0%
!! CAAAACCGGCTTGTAAGGTG+AGG + Chr2:57505787-57505806 MsG0280009749.01.T01:intron 50.0%
!! CCACCTTGCTGTTTCACATG+AGG - Chr2:57507422-57507441 None:intergenic 50.0%
!! GATTCTGCGGATTGGGCATT+GGG + Chr2:57507119-57507138 MsG0280009749.01.T01:CDS 50.0%
!! GGCATTGGGAAAGGTACTTC+TGG + Chr2:57507133-57507152 MsG0280009749.01.T01:intron 50.0%
!! GGCTTGGTGCGTGAATATGT+AGG + Chr2:57505908-57505927 MsG0280009749.01.T01:intron 50.0%
!!! AGAACCTGCTTTTGGGGTGA+TGG - Chr2:57506728-57506747 None:intergenic 50.0%
AGAGCCTCAAGTGGTCCCTT+AGG - Chr2:57507452-57507471 None:intergenic 55.0%
AGGGACCACTTGAGGCTCTT+CGG + Chr2:57507453-57507472 MsG0280009749.01.T01:intron 55.0%
AGTAAGCACGCTCATGGTCC+TGG - Chr2:57507097-57507116 None:intergenic 55.0%
ATCGGACACGACACTGACAC+CGG - Chr2:57504185-57504204 None:intergenic 55.0%
CATGCCATCACCCCAAAAGC+AGG + Chr2:57506721-57506740 MsG0280009749.01.T01:CDS 55.0%
CGCAGCAGCAAACACGTTAC+CGG + Chr2:57508176-57508195 MsG0280009749.01.T01:CDS 55.0%
GAAGCAGCAACCGTGATGAG+CGG - Chr2:57503859-57503878 None:intergenic 55.0%
GAGCTCAACTCAACACCGAC+CGG + Chr2:57503821-57503840 MsG0280009749.01.T01:five_prime_UTR 55.0%
GGCTCTTCGGCCTAAACTAC+AGG + Chr2:57507466-57507485 MsG0280009749.01.T01:intron 55.0%
TTAGGCCGAAGAGCCTCAAG+TGG - Chr2:57507461-57507480 None:intergenic 55.0%
! CTCTCCGAAGCTGTTGATGG+AGG - Chr2:57504835-57504854 None:intergenic 55.0%
! CTTCACCGGAAGGAGCGTAA+GGG - Chr2:57508207-57508226 None:intergenic 55.0%
! GCTATAGCTCAGTGGTGACG+AGG - Chr2:57504751-57504770 None:intergenic 55.0%
! TGCTGCTTCCCTCACATCTC+CGG + Chr2:57503870-57503889 MsG0280009749.01.T01:five_prime_UTR 55.0%
! TTCACCGGAAGGAGCGTAAG+GGG - Chr2:57508206-57508225 None:intergenic 55.0%
!! CACCAAGCCCAAAGAGTGCT+GGG - Chr2:57505897-57505916 None:intergenic 55.0%
!! CGATTCTGCGGATTGGGCAT+TGG + Chr2:57507118-57507137 MsG0280009749.01.T01:CDS 55.0%
!! TGCGGATTGGGCATTGGGAA+AGG + Chr2:57507124-57507143 MsG0280009749.01.T01:CDS 55.0%
CCTCACGCCCAGCACTCTTT+GGG + Chr2:57505887-57505906 MsG0280009749.01.T01:intron 60.0%
CCTTACGCTCCTTCCGGTGA+AGG + Chr2:57508205-57508224 MsG0280009749.01.T01:CDS 60.0%
CTCAACACCGACCGGACGTT+CGG + Chr2:57503829-57503848 MsG0280009749.01.T01:five_prime_UTR 60.0%
GCGTGTACGGTGGTGTACCT+TGG - Chr2:57506944-57506963 None:intergenic 60.0%
! CCTTCACCGGAAGGAGCGTA+AGG - Chr2:57508208-57508227 None:intergenic 60.0%
! GCACCAAGCCCAAAGAGTGC+TGG - Chr2:57505898-57505917 None:intergenic 60.0%
!! AAAGAGTGCTGGGCGTGAGG+GGG - Chr2:57505887-57505906 None:intergenic 60.0%
!! CAAAGAGTGCTGGGCGTGAG+GGG - Chr2:57505888-57505907 None:intergenic 60.0%
!! CCAAAGAGTGCTGGGCGTGA+GGG - Chr2:57505889-57505908 None:intergenic 60.0%
!! GTTCGGTGCTCCGCTCATCA+CGG + Chr2:57503846-57503865 MsG0280009749.01.T01:five_prime_UTR 60.0%
CCCTCACGCCCAGCACTCTT+TGG + Chr2:57505886-57505905 MsG0280009749.01.T01:intron 65.0%
CGCCCAGCACTCTTTGGGCT+TGG + Chr2:57505892-57505911 MsG0280009749.01.T01:intron 65.0%
GGACACGACACTGACACCGG+CGG - Chr2:57504182-57504201 None:intergenic 65.0%
! GGGCGTGAGGGGGGTGTATT+GGG - Chr2:57505877-57505896 None:intergenic 65.0%
! TGGGCGTGAGGGGGGTGTAT+TGG - Chr2:57505878-57505897 None:intergenic 65.0%
!! AAGAGTGCTGGGCGTGAGGG+GGG - Chr2:57505886-57505905 None:intergenic 65.0%
!! CCCAAAGAGTGCTGGGCGTG+AGG - Chr2:57505890-57505909 None:intergenic 65.0%
!! TGAGCGGAGCACCGAACGTC+CGG - Chr2:57503843-57503862 None:intergenic 65.0%
!! CGGAGCACCGAACGTCCGGT+CGG - Chr2:57503839-57503858 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 57503778 57508685 57503778 ID=MsG0280009749.01;Name=MsG0280009749.01
Chr2 mRNA 57503778 57508685 57503778 ID=MsG0280009749.01.T01;Parent=MsG0280009749.01;Name=MsG0280009749.01.T01;_AED=0.43;_eAED=0.43;_QI=137|0.8|1|1|0.8|0.66|6|315|99
Chr2 exon 57503778 57503891 57503778 ID=MsG0280009749.01.T01:exon:18712;Parent=MsG0280009749.01.T01
Chr2 exon 57506630 57506742 57506630 ID=MsG0280009749.01.T01:exon:18713;Parent=MsG0280009749.01.T01
Chr2 exon 57506883 57506945 57506883 ID=MsG0280009749.01.T01:exon:18714;Parent=MsG0280009749.01.T01
Chr2 exon 57507098 57507145 57507098 ID=MsG0280009749.01.T01:exon:18715;Parent=MsG0280009749.01.T01
Chr2 exon 57508172 57508226 57508172 ID=MsG0280009749.01.T01:exon:18716;Parent=MsG0280009749.01.T01
Chr2 exon 57508327 57508685 57508327 ID=MsG0280009749.01.T01:exon:18717;Parent=MsG0280009749.01.T01
Chr2 five_prime_UTR 57503778 57503891 57503778 ID=MsG0280009749.01.T01:five_prime_utr;Parent=MsG0280009749.01.T01
Chr2 five_prime_UTR 57506630 57506652 57506630 ID=MsG0280009749.01.T01:five_prime_utr;Parent=MsG0280009749.01.T01
Chr2 CDS 57506653 57506742 57506653 ID=MsG0280009749.01.T01:cds;Parent=MsG0280009749.01.T01
Chr2 CDS 57506883 57506945 57506883 ID=MsG0280009749.01.T01:cds;Parent=MsG0280009749.01.T01
Chr2 CDS 57507098 57507145 57507098 ID=MsG0280009749.01.T01:cds;Parent=MsG0280009749.01.T01
Chr2 CDS 57508172 57508226 57508172 ID=MsG0280009749.01.T01:cds;Parent=MsG0280009749.01.T01
Chr2 CDS 57508327 57508370 57508327 ID=MsG0280009749.01.T01:cds;Parent=MsG0280009749.01.T01
Chr2 three_prime_UTR 57508371 57508685 57508371 ID=MsG0280009749.01.T01:three_prime_utr;Parent=MsG0280009749.01.T01
Gene Sequence

>MsG0280009749.01.T01

ATGTCAAACATAGAGGAAAACAAAGACATTGGACAGATGGATGTGGATGCATCTGATGGGTCAGTTAACATGCCATCACCCCAAAAGCAGGAGGAAACTTTAAAGAAAAAATATGGAGGAATTGTACCCAAAAAGCCACCACTTATTTCCAAGGACCATGAGCGTGCTTACTTCGATTCTGCGGATTGGGCATTGGGAAAGCCAACGCAGCAGCAAACACGTTACCGGAAATCCCCTTACGCTCCTTCCGGTGAAGAAGGTGGAAGTGTTCCATCTGAGGATGCAGCTTCCAATGAGTAA
Protein sequence

>MsG0280009749.01.T01

MSNIEENKDIGQMDVDASDGSVNMPSPQKQEETLKKKYGGIVPKKPPLISKDHERAYFDSADWALGKPTQQQTRYRKSPYAPSGEEGGSVPSEDAASNE*