AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004658.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004658.01.T01 MTR_1g084040 94.882 254 6 1 23 276 25 271 4.66e-176 486
MsG0180004658.01.T01 MTR_7g112110 75.824 182 44 0 23 204 27 208 8.35e-102 296
MsG0180004658.01.T01 MTR_7g112120 74.033 181 46 1 23 202 26 206 5.66e-96 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004658.01.T01 AT1G56423 58.537 246 85 4 23 268 23 251 2.53e-98 288
MsG0180004658.01.T01 AT1G56423 57.812 256 89 5 23 268 23 269 2.62e-98 289

Find 73 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGATTTATGATGTGATTAA+TGG 0.186748 1:+80874091 MsG0180004658.01.T01:CDS
GGCACTTTGTTTGGTGCATA+TGG 0.213119 1:+80873977 MsG0180004658.01.T01:CDS
ATTTGAAGTTCATATAATTT+AGG 0.252241 1:+80874360 MsG0180004658.01.T01:three_prime_UTR
CGTCAAGCCAAGCGGATTAT+AGG 0.285681 1:+80873872 MsG0180004658.01.T01:CDS
AGGGGTACTGGCACTTTGTT+TGG 0.287164 1:+80873968 MsG0180004658.01.T01:CDS
TGACATGGGCTCTGGAACTT+TGG 0.287746 1:+80873667 MsG0180004658.01.T01:CDS
TCACCATCAAAAGAAGTATA+TGG 0.340203 1:-80874193 None:intergenic
CCTTGTTGACGCTGTTAGTC+AGG 0.341491 1:+80873781 MsG0180004658.01.T01:CDS
ATAGGCCCCATCATCTCTTC+TGG 0.347557 1:+80873890 MsG0180004658.01.T01:CDS
GCACTTTGTTTGGTGCATAT+GGG 0.347749 1:+80873978 MsG0180004658.01.T01:CDS
CAATCACAGAAGGGTTCCTT+AGG 0.357038 1:+80873948 MsG0180004658.01.T01:CDS
ATCCAATGTGGATGAATCTA+TGG 0.357729 1:+80874297 MsG0180004658.01.T01:CDS
AAGAGTGGTATCTTCGATTC+TGG 0.372703 1:+80871291 MsG0180004658.01.T01:CDS
GGCAATGAATGGGGCATTGA+TGG 0.376015 1:+80871246 MsG0180004658.01.T01:CDS
GATGGCGAAGTTCCTACTTT+TGG 0.378746 1:+80874229 MsG0180004658.01.T01:CDS
TGCGCAGAAAGAAAGTAAAA+AGG 0.400376 1:+80873832 MsG0180004658.01.T01:CDS
AGTTGGGTTGGTGGCAGAAT+AGG 0.401598 1:+80874064 MsG0180004658.01.T01:CDS
TAATGGAGTGCATTTGTTGC+TGG 0.410499 1:+80874108 MsG0180004658.01.T01:CDS
GAGCAAAGCCTTGGTAGAAT+TGG 0.427351 1:+80874016 MsG0180004658.01.T01:CDS
GGTAGAATTGGCTATCTTGT+TGG 0.428206 1:+80874028 MsG0180004658.01.T01:CDS
GGGAGATGATTCATATGAAC+TGG 0.428386 1:-80874266 None:intergenic
CACTTTGTTTGGTGCATATG+GGG 0.431463 1:+80873979 MsG0180004658.01.T01:CDS
TTGGAAGTCACATGGGCAGT+TGG 0.444851 1:+80874047 MsG0180004658.01.T01:CDS
GGATGAAATGGGCAATGAAT+GGG 0.450825 1:+80871236 None:intergenic
AGTCACATGGGCAGTTGGGT+TGG 0.461400 1:+80874052 MsG0180004658.01.T01:CDS
GAAGGGTTCCTTAGGGGTAC+TGG 0.461508 1:+80873956 MsG0180004658.01.T01:CDS
TTGCTGGAATTTGTTCAAAC+AGG 0.465120 1:+80874124 MsG0180004658.01.T01:CDS
AGGATGAAATGGGCAATGAA+TGG 0.474811 1:+80871235 None:intergenic
CACATGGGCAGTTGGGTTGG+TGG 0.487418 1:+80874055 MsG0180004658.01.T01:CDS
TGGAAGTCACATGGGCAGTT+GGG 0.489000 1:+80874048 MsG0180004658.01.T01:CDS
TAATGTGAACTTTCCACTCT+GGG 0.493495 1:-80873633 None:intergenic
CTTGTTGACGCTGTTAGTCA+GGG 0.493758 1:+80873782 MsG0180004658.01.T01:CDS
CCCCATCATCTCTTCTGGAT+GGG 0.496655 1:+80873895 MsG0180004658.01.T01:CDS
TTCTTCGGGGAGCAAAGCCT+TGG 0.501943 1:+80874007 MsG0180004658.01.T01:CDS
TTCGAAGCTATTTATTATGG+TGG 0.502729 1:+80873923 MsG0180004658.01.T01:CDS
TTCCATAGATTCATCCACAT+TGG 0.502813 1:-80874299 None:intergenic
TCCACATTGGATTCTTCAGT+GGG 0.511116 1:-80874286 None:intergenic
GTCCCATCCAGAAGAGATGA+TGG 0.512466 1:-80873897 None:intergenic
GCCCCATCATCTCTTCTGGA+TGG 0.519007 1:+80873894 MsG0180004658.01.T01:CDS
AGGGAGCTTATTGGCGAGGA+GGG 0.520387 1:+80873605 MsG0180004658.01.T01:CDS
ATAATGTGAACTTTCCACTC+TGG 0.524601 1:-80873634 None:intergenic
CTTTCAGAGCTGCCAAAAGT+AGG 0.529923 1:-80874241 None:intergenic
CTATCTTGTTGGAAGTCACA+TGG 0.536263 1:+80874039 MsG0180004658.01.T01:CDS
GAAGTTCATATAATTTAGGC+GGG 0.538620 1:+80874364 MsG0180004658.01.T01:three_prime_UTR
AACTTTGGCATGTGCTGTCA+AGG 0.544459 1:+80873682 MsG0180004658.01.T01:CDS
ACTTTGTTTGGTGCATATGG+GGG 0.548774 1:+80873980 MsG0180004658.01.T01:CDS
TGAAGTTCATATAATTTAGG+CGG 0.558383 1:+80874363 MsG0180004658.01.T01:three_prime_UTR
AATCACAGAAGGGTTCCTTA+GGG 0.565296 1:+80873949 MsG0180004658.01.T01:CDS
TTGACGAGAAGCCAATTTCG+CGG 0.567474 1:-80873855 None:intergenic
TATGGTGGTACAATCACAGA+AGG 0.573148 1:+80873938 MsG0180004658.01.T01:CDS
TTGGCATGTGCTGTCAAGGA+AGG 0.578441 1:+80873686 MsG0180004658.01.T01:CDS
GATGGGGCCTATAATCCGCT+TGG 0.579892 1:-80873879 None:intergenic
TCCCATCCAGAAGAGATGAT+GGG 0.583665 1:-80873896 None:intergenic
ATCCACATTGGATTCTTCAG+TGG 0.584833 1:-80874287 None:intergenic
ACAAAGTGCCAGTACCCCTA+AGG 0.589908 1:-80873964 None:intergenic
GGAACTTCGCCATCATGAGT+TGG 0.592542 1:-80874220 None:intergenic
CCTGACTAACAGCGTCAACA+AGG 0.594656 1:-80873781 None:intergenic
AATGAATGGGGCATTGATGG+TGG 0.603155 1:+80871249 MsG0180004658.01.T01:CDS
GGGAGCTTATTGGCGAGGAG+GGG 0.620894 1:+80873606 MsG0180004658.01.T01:CDS
CCCATCCAGAAGAGATGATG+GGG 0.628978 1:-80873895 None:intergenic
GATGAAATGGGCAATGAATG+GGG 0.636105 1:+80871237 None:intergenic
TTGTTGACGCTGTTAGTCAG+GGG 0.644595 1:+80873783 MsG0180004658.01.T01:CDS
TATCTTGTTGGAAGTCACAT+GGG 0.645553 1:+80874040 MsG0180004658.01.T01:CDS
ATGCAGGGAGCTTATTGGCG+AGG 0.646429 1:+80873601 MsG0180004658.01.T01:CDS
CAGGGAGCTTATTGGCGAGG+AGG 0.648197 1:+80873604 MsG0180004658.01.T01:CDS
GGTGAAGTTCCAACTCATGA+TGG 0.654282 1:+80874211 MsG0180004658.01.T01:CDS
TGGCTTCTCGTCAAGCCAAG+CGG 0.674972 1:+80873864 MsG0180004658.01.T01:CDS
ATGGTGGTACAATCACAGAA+GGG 0.678014 1:+80873939 MsG0180004658.01.T01:CDS
CAAGATAGCCAATTCTACCA+AGG 0.680375 1:-80874024 None:intergenic
ATCACAGAAGGGTTCCTTAG+GGG 0.680466 1:+80873950 MsG0180004658.01.T01:CDS
CTGACTAACAGCGTCAACAA+GGG 0.688363 1:-80873780 None:intergenic
TCCCACTGAAGAATCCAATG+TGG 0.713284 1:+80874285 MsG0180004658.01.T01:CDS
GAGGAGGGGTCATCCCAGAG+TGG 0.752894 1:+80873620 MsG0180004658.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAAAAAAAGA+AGG - Chr1:80871347-80871366 None:intergenic 10.0%
!! AAAAAAAAAGAAAAAAAGAA+GGG - Chr1:80871346-80871365 None:intergenic 10.0%
!! AACATAAAAATACAAAAATA+TGG - Chr1:80873417-80873436 None:intergenic 10.0%
!! ATATATCATTATATTTATTC+TGG + Chr1:80873334-80873353 MsG0180004658.01.T01:intron 10.0%
!! AGAAAATGAAGAAATATAAT+TGG + Chr1:80873237-80873256 MsG0180004658.01.T01:intron 15.0%
!! TAGTAAATAAAATGATCAAT+TGG - Chr1:80872890-80872909 None:intergenic 15.0%
!! TGAAATAAAATGAAATTGAA+TGG + Chr1:80873278-80873297 MsG0180004658.01.T01:intron 15.0%
!!! ATTATATTTCTTCATTTTCT+TGG - Chr1:80873237-80873256 None:intergenic 15.0%
!!! ATTTGAAGTTCATATAATTT+AGG + Chr1:80874360-80874379 MsG0180004658.01.T01:three_prime_UTR 15.0%
!!! CAAAAATAATATTTTACTCA+TGG + Chr1:80872416-80872435 MsG0180004658.01.T01:intron 15.0%
!!! CATTTATGCTTTAAAAAAAA+GGG - Chr1:80874432-80874451 None:intergenic 15.0%
!! ATGATTTATGATGTGATTAA+TGG + Chr1:80874091-80874110 MsG0180004658.01.T01:CDS 20.0%
!! TAACAAAAAAAATAATCTCG+TGG - Chr1:80871371-80871390 None:intergenic 20.0%
!! TTATTGGAATGAAATGAAAA+TGG + Chr1:80873385-80873404 MsG0180004658.01.T01:intron 20.0%
!!! AAATAATATTTTACTCATGG+TGG + Chr1:80872419-80872438 MsG0180004658.01.T01:intron 20.0%
!!! ATTATTAAACTGTTTTGACA+TGG + Chr1:80873652-80873671 MsG0180004658.01.T01:CDS 20.0%
!!! ATTGATGATTTTTCTGATTT+TGG + Chr1:80872927-80872946 MsG0180004658.01.T01:intron 20.0%
!!! ATTTTCATTTCATTCCAATA+AGG - Chr1:80873386-80873405 None:intergenic 20.0%
!!! GCATTTATGCTTTAAAAAAA+AGG - Chr1:80874433-80874452 None:intergenic 20.0%
!!! TAAAGAAACCAATATTTTGA+AGG + Chr1:80872195-80872214 MsG0180004658.01.T01:intron 20.0%
!!! TTATTAAACTGTTTTGACAT+GGG + Chr1:80873653-80873672 MsG0180004658.01.T01:CDS 20.0%
!!! TTGATGATTTTTCTGATTTT+GGG + Chr1:80872928-80872947 MsG0180004658.01.T01:intron 20.0%
!!! TTTTCATTTCATTCCAATAA+GGG - Chr1:80873385-80873404 None:intergenic 20.0%
!!! TTTTTCGAAGCTATTTATTA+TGG + Chr1:80873920-80873939 MsG0180004658.01.T01:CDS 20.0%
! AAAAAAAGAAGGGTTTATGA+AGG - Chr1:80871336-80871355 None:intergenic 25.0%
! AAAAAAGAAGGGTTTATGAA+GGG - Chr1:80871335-80871354 None:intergenic 25.0%
! AAAATAGTCACATATCATTG+TGG - Chr1:80873177-80873196 None:intergenic 25.0%
! AAACATATTCAAGAGGTTAA+TGG + Chr1:80871908-80871927 MsG0180004658.01.T01:intron 25.0%
! AAATATGAAGTGGTTTATAG+AGG + Chr1:80871617-80871636 MsG0180004658.01.T01:intron 25.0%
! AAATGTATGGTATAGTTGAT+TGG - Chr1:80872226-80872245 None:intergenic 25.0%
! AAGAACAAAACATATTCAAG+AGG + Chr1:80871901-80871920 MsG0180004658.01.T01:intron 25.0%
! AATGTATGGTATAGTTGATT+GGG - Chr1:80872225-80872244 None:intergenic 25.0%
! AGAATTAGTTATTATTGCCA+TGG + Chr1:80872735-80872754 MsG0180004658.01.T01:intron 25.0%
! AGAATTCTTGAAGATCTATA+AGG - Chr1:80871736-80871755 None:intergenic 25.0%
! ATAAGCTTAATTAGTGTAAG+AGG - Chr1:80872962-80872981 None:intergenic 25.0%
! ATAATGTGAATGTGTATTGT+TGG - Chr1:80872299-80872318 None:intergenic 25.0%
! GTGTGTATATGAAAAACAAT+CGG + Chr1:80872441-80872460 MsG0180004658.01.T01:intron 25.0%
! TAATTGGACATGTATAGAAT+TGG + Chr1:80873253-80873272 MsG0180004658.01.T01:intron 25.0%
! TACAAAAGTGAAATATGAAG+TGG + Chr1:80871607-80871626 MsG0180004658.01.T01:intron 25.0%
! TGAAGTTCATATAATTTAGG+CGG + Chr1:80874363-80874382 MsG0180004658.01.T01:three_prime_UTR 25.0%
!! GGCATTGCATATATTATTTT+TGG - Chr1:80871510-80871529 None:intergenic 25.0%
!! TGGTAACATAACTATTTTCT+TGG - Chr1:80871490-80871509 None:intergenic 25.0%
!!! ATACCACATTGAAAAGTATT+CGG - Chr1:80871770-80871789 None:intergenic 25.0%
!!! ATCATCATTTTTGCAATTTC+CGG - Chr1:80872155-80872174 None:intergenic 25.0%
!!! TACTTTTTAAGATGCAAGAT+TGG + Chr1:80871704-80871723 MsG0180004658.01.T01:intron 25.0%
!!! TGACTCAGTATATTTTAGTT+CGG - Chr1:80872489-80872508 None:intergenic 25.0%
AAAAAGAAGGGTTTATGAAG+GGG - Chr1:80871334-80871353 None:intergenic 30.0%
ATAAGCTCACAAACATAATG+AGG + Chr1:80871565-80871584 MsG0180004658.01.T01:intron 30.0%
GAACTAAAATATACTGAGTC+AGG + Chr1:80872488-80872507 MsG0180004658.01.T01:intron 30.0%
GAAGTTCATATAATTTAGGC+GGG + Chr1:80874364-80874383 MsG0180004658.01.T01:three_prime_UTR 30.0%
GATGATACTCAAAGAAATCA+TGG + Chr1:80872169-80872188 MsG0180004658.01.T01:intron 30.0%
GTAAAACTCTCAAACAAGTT+TGG - Chr1:80871687-80871706 None:intergenic 30.0%
TACAACAATAACTCCTTTAG+AGG + Chr1:80871836-80871855 MsG0180004658.01.T01:intron 30.0%
TTCGAAGCTATTTATTATGG+TGG + Chr1:80873923-80873942 MsG0180004658.01.T01:CDS 30.0%
! AATCCGAATACTTTTCAATG+TGG + Chr1:80871764-80871783 MsG0180004658.01.T01:intron 30.0%
! CTATACCATACATTTTCTCT+AGG + Chr1:80872231-80872250 MsG0180004658.01.T01:intron 30.0%
! GGTTAACTTTAAAGATGACA+GGG + Chr1:80872382-80872401 MsG0180004658.01.T01:intron 30.0%
! TCACCATCAAAAGAAGTATA+TGG - Chr1:80874196-80874215 None:intergenic 30.0%
!!! ATGCATTGCATGTGTTTTTT+AGG - Chr1:80871458-80871477 None:intergenic 30.0%
!!! TCAAGAGTATTTTCCTCTAA+AGG - Chr1:80871852-80871871 None:intergenic 30.0%
!!! TGCATTGCATGTGTTTTTTA+GGG - Chr1:80871457-80871476 None:intergenic 30.0%
AACGGTCAATTTCGAAAGTT+CGG + Chr1:80872596-80872615 MsG0180004658.01.T01:intron 35.0%
ATAATGTGAACTTTCCACTC+TGG - Chr1:80873637-80873656 None:intergenic 35.0%
ATCCAATGTGGATGAATCTA+TGG + Chr1:80874297-80874316 MsG0180004658.01.T01:CDS 35.0%
ATGGACCTAGAGAAAATGTA+TGG - Chr1:80872239-80872258 None:intergenic 35.0%
ATTCCAATAAGGGAGGAATA+TGG - Chr1:80873375-80873394 None:intergenic 35.0%
ATTGCAAAGTACGTTCTTGA+TGG - Chr1:80872014-80872033 None:intergenic 35.0%
ATTTCCAAAACAGAAAGGCT+CGG + Chr1:80872758-80872777 MsG0180004658.01.T01:intron 35.0%
CAATTTCATGCACAAGTCTA+AGG - Chr1:80872268-80872287 None:intergenic 35.0%
CACAAGTCTAAGGAGAATAA+TGG - Chr1:80872258-80872277 None:intergenic 35.0%
CATAACATTCTTCCTTCTCT+TGG + Chr1:80872653-80872672 MsG0180004658.01.T01:intron 35.0%
CATATTCCATAACAAAGAGC+CGG + Chr1:80872133-80872152 MsG0180004658.01.T01:intron 35.0%
CATGGATTTCCAAAACAGAA+AGG + Chr1:80872753-80872772 MsG0180004658.01.T01:intron 35.0%
CCTAAGCTATTTATCCAATC+AGG + Chr1:80872355-80872374 MsG0180004658.01.T01:intron 35.0%
CCTGATTGGATAAATAGCTT+AGG - Chr1:80872358-80872377 None:intergenic 35.0%
CGTGAAAAGATTATTCGCTA+CGG + Chr1:80872321-80872340 MsG0180004658.01.T01:intron 35.0%
CTACTCATGTTGAAAAAGCA+AGG + Chr1:80873738-80873757 MsG0180004658.01.T01:CDS 35.0%
GACTTGTGCATGAAATTGTA+TGG + Chr1:80872270-80872289 MsG0180004658.01.T01:intron 35.0%
GATACTCAAAGAAATCATGG+AGG + Chr1:80872172-80872191 MsG0180004658.01.T01:intron 35.0%
GCTTATGTGAGAAATTTAGC+CGG - Chr1:80871551-80871570 None:intergenic 35.0%
GGCTACATATAGCAATGTTT+AGG - Chr1:80873049-80873068 None:intergenic 35.0%
TAATGTGAACTTTCCACTCT+GGG - Chr1:80873636-80873655 None:intergenic 35.0%
TATCTTGTTGGAAGTCACAT+GGG + Chr1:80874040-80874059 MsG0180004658.01.T01:CDS 35.0%
TCATATTTGTGCTTGTGATC+TGG + Chr1:80872990-80873009 MsG0180004658.01.T01:intron 35.0%
TCATTTCATTCCAATAAGGG+AGG - Chr1:80873382-80873401 None:intergenic 35.0%
TGCGCAGAAAGAAAGTAAAA+AGG + Chr1:80873832-80873851 MsG0180004658.01.T01:CDS 35.0%
TGGGAGTTCCTTCAAAATAT+TGG - Chr1:80872206-80872225 None:intergenic 35.0%
TTCCAATAAGGGAGGAATAT+GGG - Chr1:80873374-80873393 None:intergenic 35.0%
TTCCATAGATTCATCCACAT+TGG - Chr1:80874302-80874321 None:intergenic 35.0%
TTGTTAATTGCCTGGAACTT+GGG + Chr1:80872527-80872546 MsG0180004658.01.T01:intron 35.0%
! CGGTTAACTTTAAAGATGAC+AGG + Chr1:80872381-80872400 MsG0180004658.01.T01:intron 35.0%
! GCTCCATATACTTCTTTTGA+TGG + Chr1:80874190-80874209 MsG0180004658.01.T01:CDS 35.0%
! TGACATTCGATTTGTGATCA+GGG + Chr1:80872855-80872874 MsG0180004658.01.T01:intron 35.0%
! TGGAGTGAACAAGTTTTCAA+GGG - Chr1:80873217-80873236 None:intergenic 35.0%
! TTGACATTCGATTTGTGATC+AGG + Chr1:80872854-80872873 MsG0180004658.01.T01:intron 35.0%
! TTGCTGGAATTTGTTCAAAC+AGG + Chr1:80874124-80874143 MsG0180004658.01.T01:CDS 35.0%
! TTGGAGTGAACAAGTTTTCA+AGG - Chr1:80873218-80873237 None:intergenic 35.0%
!! GTCATCTTTAAAGTTAACCG+TGG - Chr1:80872381-80872400 None:intergenic 35.0%
!!! CAATATTTTGACCTGCCATA+AGG + Chr1:80871978-80871997 MsG0180004658.01.T01:intron 35.0%
!!! CTTTCTGTTTTGGAAATCCA+TGG - Chr1:80872755-80872774 None:intergenic 35.0%
AACAACACGTGATCTCACTT+AGG - Chr1:80873070-80873089 None:intergenic 40.0%
AAGAGTGGTATCTTCGATTC+TGG + Chr1:80871291-80871310 MsG0180004658.01.T01:CDS 40.0%
AAGTAAAAAGGCCGCGAAAT+TGG + Chr1:80873844-80873863 MsG0180004658.01.T01:CDS 40.0%
AATCACAGAAGGGTTCCTTA+GGG + Chr1:80873949-80873968 MsG0180004658.01.T01:CDS 40.0%
AATCACTATTACCCTCTTCG+TGG - Chr1:80872104-80872123 None:intergenic 40.0%
ACAAGATACTCCACAGTTCT+CGG + Chr1:80873018-80873037 MsG0180004658.01.T01:intron 40.0%
ATCCACATTGGATTCTTCAG+TGG - Chr1:80874290-80874309 None:intergenic 40.0%
ATCCCATATTCCTCCCTTAT+TGG + Chr1:80873369-80873388 MsG0180004658.01.T01:intron 40.0%
ATGGTGGTACAATCACAGAA+GGG + Chr1:80873939-80873958 MsG0180004658.01.T01:CDS 40.0%
CAAGATAGCCAATTCTACCA+AGG - Chr1:80874027-80874046 None:intergenic 40.0%
CAATTTCCGGCTCTTTGTTA+TGG - Chr1:80872142-80872161 None:intergenic 40.0%
CTATCTTGTTGGAAGTCACA+TGG + Chr1:80874039-80874058 MsG0180004658.01.T01:CDS 40.0%
CTATTTATCCAATCAGGCCA+CGG + Chr1:80872361-80872380 MsG0180004658.01.T01:intron 40.0%
GAAAAAATGAGGGAGAAGAG+TGG + Chr1:80871276-80871295 MsG0180004658.01.T01:CDS 40.0%
GAAAAATGCAGGGAGCTTAT+TGG + Chr1:80873596-80873615 MsG0180004658.01.T01:CDS 40.0%
GCTTTCAGCTGAAAAATGCA+GGG + Chr1:80873586-80873605 MsG0180004658.01.T01:intron 40.0%
GGGAGATGATTCATATGAAC+TGG - Chr1:80874269-80874288 None:intergenic 40.0%
GGTAGAATTGGCTATCTTGT+TGG + Chr1:80874028-80874047 MsG0180004658.01.T01:CDS 40.0%
GTTGTTAATTGCCTGGAACT+TGG + Chr1:80872526-80872545 MsG0180004658.01.T01:intron 40.0%
TATGGTGGTACAATCACAGA+AGG + Chr1:80873938-80873957 MsG0180004658.01.T01:CDS 40.0%
TCCACATTGGATTCTTCAGT+GGG - Chr1:80874289-80874308 None:intergenic 40.0%
TGCTTTCAGCTGAAAAATGC+AGG + Chr1:80873585-80873604 MsG0180004658.01.T01:intron 40.0%
TGGTGGAAGCGAAAAAATGA+GGG + Chr1:80871266-80871285 MsG0180004658.01.T01:CDS 40.0%
! ATAACCGAGCCTTTCTGTTT+TGG - Chr1:80872765-80872784 None:intergenic 40.0%
! ATGGTGGAAGCGAAAAAATG+AGG + Chr1:80871265-80871284 MsG0180004658.01.T01:CDS 40.0%
! ATGTTTTGCTGCATCTGTTG+GGG - Chr1:80873813-80873832 None:intergenic 40.0%
! CATACAGTCTTTTGTTGCTG+AGG - Chr1:80873454-80873473 None:intergenic 40.0%
! CATGTTTTGCTGCATCTGTT+GGG - Chr1:80873814-80873833 None:intergenic 40.0%
! GACATTCGATTTGTGATCAG+GGG + Chr1:80872856-80872875 MsG0180004658.01.T01:intron 40.0%
! TAATGGAGTGCATTTGTTGC+TGG + Chr1:80874108-80874127 MsG0180004658.01.T01:CDS 40.0%
!! ACTTTGTTTGGTGCATATGG+GGG + Chr1:80873980-80873999 MsG0180004658.01.T01:CDS 40.0%
!! CACTTTGTTTGGTGCATATG+GGG + Chr1:80873979-80873998 MsG0180004658.01.T01:CDS 40.0%
!! CTCTCAAACAAGTTTGGTGT+TGG - Chr1:80871681-80871700 None:intergenic 40.0%
!! GCACTTTGTTTGGTGCATAT+GGG + Chr1:80873978-80873997 MsG0180004658.01.T01:CDS 40.0%
!!! CGTTTTGAAGACAGTTCGAA+TGG - Chr1:80872681-80872700 None:intergenic 40.0%
AAAGTTAACCGTGGCCTGAT+TGG - Chr1:80872372-80872391 None:intergenic 45.0%
AAAGTTCGGCGAGAAAGCAA+AGG + Chr1:80872610-80872629 MsG0180004658.01.T01:intron 45.0%
AACTTTGGCATGTGCTGTCA+AGG + Chr1:80873682-80873701 MsG0180004658.01.T01:CDS 45.0%
AGACCATCAACTACACTGTC+TGG + Chr1:80873533-80873552 MsG0180004658.01.T01:intron 45.0%
ATCACAGAAGGGTTCCTTAG+GGG + Chr1:80873950-80873969 MsG0180004658.01.T01:CDS 45.0%
ATCGTTGCTCTTAGCATCAC+AGG + Chr1:80872802-80872821 MsG0180004658.01.T01:intron 45.0%
CAATCACAGAAGGGTTCCTT+AGG + Chr1:80873948-80873967 MsG0180004658.01.T01:CDS 45.0%
CTACCAGACAGTGTAGTTGA+TGG - Chr1:80873539-80873558 None:intergenic 45.0%
CTGACTAACAGCGTCAACAA+GGG - Chr1:80873783-80873802 None:intergenic 45.0%
CTTGACAAAGACCACGAAGA+GGG + Chr1:80872090-80872109 MsG0180004658.01.T01:intron 45.0%
CTTGTTGACGCTGTTAGTCA+GGG + Chr1:80873782-80873801 MsG0180004658.01.T01:CDS 45.0%
CTTTCAGAGCTGCCAAAAGT+AGG - Chr1:80874244-80874263 None:intergenic 45.0%
GAGCAAAGCCTTGGTAGAAT+TGG + Chr1:80874016-80874035 MsG0180004658.01.T01:CDS 45.0%
GATGGTGATCGACAACCTTA+TGG - Chr1:80871996-80872015 None:intergenic 45.0%
GGTGAAGTTCCAACTCATGA+TGG + Chr1:80874211-80874230 MsG0180004658.01.T01:CDS 45.0%
GTTCGAATGGTTCCAAGAGA+AGG - Chr1:80872668-80872687 None:intergenic 45.0%
TAATTGCCTGGAACTTGGGT+GGG + Chr1:80872531-80872550 MsG0180004658.01.T01:intron 45.0%
TCCCACTGAAGAATCCAATG+TGG + Chr1:80874285-80874304 MsG0180004658.01.T01:CDS 45.0%
TCCCATCCAGAAGAGATGAT+GGG - Chr1:80873899-80873918 None:intergenic 45.0%
TCTTGACAAAGACCACGAAG+AGG + Chr1:80872089-80872108 MsG0180004658.01.T01:intron 45.0%
TGAGAAATTTAGCCGGTGTG+TGG - Chr1:80871544-80871563 None:intergenic 45.0%
TGTGATGCTAAGAGCAACGA+TGG - Chr1:80872803-80872822 None:intergenic 45.0%
TTAATTGCCTGGAACTTGGG+TGG + Chr1:80872530-80872549 MsG0180004658.01.T01:intron 45.0%
TTAGGATCTACCGAGAACTG+TGG - Chr1:80873031-80873050 None:intergenic 45.0%
TTGACGAGAAGCCAATTTCG+CGG - Chr1:80873858-80873877 None:intergenic 45.0%
TTGTTGACGCTGTTAGTCAG+GGG + Chr1:80873783-80873802 MsG0180004658.01.T01:CDS 45.0%
TTTGTGCTTGTGATCTGGAG+TGG + Chr1:80872995-80873014 MsG0180004658.01.T01:intron 45.0%
! ATTCGATTTGTGATCAGGGG+AGG + Chr1:80872859-80872878 MsG0180004658.01.T01:intron 45.0%
! GATGGCGAAGTTCCTACTTT+TGG + Chr1:80874229-80874248 MsG0180004658.01.T01:CDS 45.0%
! GCATGTTTTGCTGCATCTGT+TGG - Chr1:80873815-80873834 None:intergenic 45.0%
! TGTTTTGCTGCATCTGTTGG+GGG - Chr1:80873812-80873831 None:intergenic 45.0%
!! AATGAATGGGGCATTGATGG+TGG + Chr1:80871249-80871268 MsG0180004658.01.T01:CDS 45.0%
!! GGCACTTTGTTTGGTGCATA+TGG + Chr1:80873977-80873996 MsG0180004658.01.T01:CDS 45.0%
!!! AACTGTTTTGACATGGGCTC+TGG + Chr1:80873659-80873678 MsG0180004658.01.T01:CDS 45.0%
ATAGGCCCCATCATCTCTTC+TGG + Chr1:80873890-80873909 MsG0180004658.01.T01:CDS 50.0%
ATCCGTGTTTGAGCGGTCAA+CGG + Chr1:80872578-80872597 MsG0180004658.01.T01:intron 50.0%
CCAGGCAATTAACAACCCGT+GGG - Chr1:80872522-80872541 None:intergenic 50.0%
CCCATCCAGAAGAGATGATG+GGG - Chr1:80873898-80873917 None:intergenic 50.0%
CCCCATCATCTCTTCTGGAT+GGG + Chr1:80873895-80873914 MsG0180004658.01.T01:CDS 50.0%
CCTGACTAACAGCGTCAACA+AGG - Chr1:80873784-80873803 None:intergenic 50.0%
CCTTGTTGACGCTGTTAGTC+AGG + Chr1:80873781-80873800 MsG0180004658.01.T01:CDS 50.0%
CTTAGTCTCTCACCACACAC+CGG + Chr1:80871529-80871548 MsG0180004658.01.T01:intron 50.0%
GATGCTAAGAGCAACGATGG+CGG - Chr1:80872800-80872819 None:intergenic 50.0%
GGAACTTCGCCATCATGAGT+TGG - Chr1:80874223-80874242 None:intergenic 50.0%
GTCCCATCCAGAAGAGATGA+TGG - Chr1:80873900-80873919 None:intergenic 50.0%
GTGATCGACAACCTTATGGC+AGG - Chr1:80871992-80872011 None:intergenic 50.0%
TCCAGGCAATTAACAACCCG+TGG - Chr1:80872523-80872542 None:intergenic 50.0%
TCGGTCAATCCGTGTTTGAG+CGG + Chr1:80872571-80872590 MsG0180004658.01.T01:intron 50.0%
TGGAAGTCACATGGGCAGTT+GGG + Chr1:80874048-80874067 MsG0180004658.01.T01:CDS 50.0%
TTGGAAGTCACATGGGCAGT+TGG + Chr1:80874047-80874066 MsG0180004658.01.T01:CDS 50.0%
TTGGCATGTGCTGTCAAGGA+AGG + Chr1:80873686-80873705 MsG0180004658.01.T01:CDS 50.0%
! ACAAAGTGCCAGTACCCCTA+AGG - Chr1:80873967-80873986 None:intergenic 50.0%
!! AGGGGTACTGGCACTTTGTT+TGG + Chr1:80873968-80873987 MsG0180004658.01.T01:CDS 50.0%
!! AGTCAGGCTTTAAAGCCCAC+GGG + Chr1:80872504-80872523 MsG0180004658.01.T01:intron 50.0%
!! AGTTGGGTTGGTGGCAGAAT+AGG + Chr1:80874064-80874083 MsG0180004658.01.T01:CDS 50.0%
!! CGTCAAGCCAAGCGGATTAT+AGG + Chr1:80873872-80873891 MsG0180004658.01.T01:CDS 50.0%
!! GAGTCAGGCTTTAAAGCCCA+CGG + Chr1:80872503-80872522 MsG0180004658.01.T01:intron 50.0%
!! GGCAATGAATGGGGCATTGA+TGG + Chr1:80871246-80871265 MsG0180004658.01.T01:CDS 50.0%
!! TGACATGGGCTCTGGAACTT+TGG + Chr1:80873667-80873686 MsG0180004658.01.T01:CDS 50.0%
!! TGGGTGGGTTGGTTCCTTTA+CGG + Chr1:80872546-80872565 MsG0180004658.01.T01:intron 50.0%
!! TGTTTGGTGCATATGGGGGA+GGG + Chr1:80873984-80874003 MsG0180004658.01.T01:CDS 50.0%
!! TTGTTTGGTGCATATGGGGG+AGG + Chr1:80873983-80874002 MsG0180004658.01.T01:CDS 50.0%
AACCAACCCACCCAAGTTCC+AGG - Chr1:80872540-80872559 None:intergenic 55.0%
AGGGAGCTTATTGGCGAGGA+GGG + Chr1:80873605-80873624 MsG0180004658.01.T01:CDS 55.0%
AGTCACATGGGCAGTTGGGT+TGG + Chr1:80874052-80874071 MsG0180004658.01.T01:CDS 55.0%
ATATGGGGGAGGGTTCTTCG+GGG + Chr1:80873994-80874013 MsG0180004658.01.T01:CDS 55.0%
ATGCAGGGAGCTTATTGGCG+AGG + Chr1:80873601-80873620 MsG0180004658.01.T01:CDS 55.0%
CATATGGGGGAGGGTTCTTC+GGG + Chr1:80873993-80874012 MsG0180004658.01.T01:CDS 55.0%
CGGTGTGTGGTGAGAGACTA+AGG - Chr1:80871531-80871550 None:intergenic 55.0%
GACCGTTGACCGCTCAAACA+CGG - Chr1:80872583-80872602 None:intergenic 55.0%
GATGGGGCCTATAATCCGCT+TGG - Chr1:80873882-80873901 None:intergenic 55.0%
GCATATGGGGGAGGGTTCTT+CGG + Chr1:80873992-80874011 MsG0180004658.01.T01:CDS 55.0%
GCCCCATCATCTCTTCTGGA+TGG + Chr1:80873894-80873913 MsG0180004658.01.T01:CDS 55.0%
TGGCTTCTCGTCAAGCCAAG+CGG + Chr1:80873864-80873883 MsG0180004658.01.T01:CDS 55.0%
TTCTTCGGGGAGCAAAGCCT+TGG + Chr1:80874007-80874026 MsG0180004658.01.T01:CDS 55.0%
! CCCACGGGTTGTTAATTGCC+TGG + Chr1:80872519-80872538 MsG0180004658.01.T01:intron 55.0%
! GAAGGGTTCCTTAGGGGTAC+TGG + Chr1:80873956-80873975 MsG0180004658.01.T01:CDS 55.0%
! GGTTGGTTCCTTTACGGCGT+CGG + Chr1:80872552-80872571 MsG0180004658.01.T01:intron 55.0%
CAGGGAGCTTATTGGCGAGG+AGG + Chr1:80873604-80873623 MsG0180004658.01.T01:CDS 60.0%
CGGATTGACCGACGCCGTAA+AGG - Chr1:80872563-80872582 None:intergenic 60.0%
GGGAGCTTATTGGCGAGGAG+GGG + Chr1:80873606-80873625 MsG0180004658.01.T01:CDS 60.0%
TGCCTGGAACTTGGGTGGGT+TGG + Chr1:80872535-80872554 MsG0180004658.01.T01:intron 60.0%
!! CACATGGGCAGTTGGGTTGG+TGG + Chr1:80874055-80874074 MsG0180004658.01.T01:CDS 60.0%
GAGGAGGGGTCATCCCAGAG+TGG + Chr1:80873620-80873639 MsG0180004658.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 80871243 80874463 80871243 ID=MsG0180004658.01;Name=MsG0180004658.01
Chr1 mRNA 80871243 80874463 80871243 ID=MsG0180004658.01.T01;Parent=MsG0180004658.01;Name=MsG0180004658.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0|0.5|0.5|1|1|2|109|276
Chr1 exon 80871243 80871312 80871243 ID=MsG0180004658.01.T01:exon:34203;Parent=MsG0180004658.01.T01
Chr1 exon 80873594 80874463 80873594 ID=MsG0180004658.01.T01:exon:34204;Parent=MsG0180004658.01.T01
Chr1 CDS 80871243 80871312 80871243 ID=MsG0180004658.01.T01:cds;Parent=MsG0180004658.01.T01
Chr1 CDS 80873594 80874354 80873594 ID=MsG0180004658.01.T01:cds;Parent=MsG0180004658.01.T01
Chr1 three_prime_UTR 80874355 80874463 80874355 ID=MsG0180004658.01.T01:three_prime_utr;Parent=MsG0180004658.01.T01
Gene Sequence

>MsG0180004658.01.T01

ATGGGCAATGAATGGGGCATTGATGGTGGAAGCGAAAAAATGAGGGAGAAGAGTGGTATCTTCGATTCTGCTGAAAAATGCAGGGAGCTTATTGGCGAGGAGGGGTCATCCCAGAGTGGAAAGTTCACATTATTAAACTGTTTTGACATGGGCTCTGGAACTTTGGCATGTGCTGTCAAGGAAGGCGTCAAACTCTATTTTTACAGTATTAGATCTACTCATGTTGAAAAAGCAAGGCAACGAGCGATCGAGTCTGCCCTTGTTGACGCTGTTAGTCAGGGGATGCCCCCAACAGATGCAGCAAAACATGCGCAGAAAGAAAGTAAAAAGGCCGCGAAATTGGCTTCTCGTCAAGCCAAGCGGATTATAGGCCCCATCATCTCTTCTGGATGGGACTTTTTCGAAGCTATTTATTATGGTGGTACAATCACAGAAGGGTTCCTTAGGGGTACTGGCACTTTGTTTGGTGCATATGGGGGAGGGTTCTTCGGGGAGCAAAGCCTTGGTAGAATTGGCTATCTTGTTGGAAGTCACATGGGCAGTTGGGTTGGTGGCAGAATAGGATTAATGATTTATGATGTGATTAATGGAGTGCATTTGTTGCTGGAATTTGTTCAAACAGGTGAAATTGAAGTTCGTAAAACATTAGTTCATGAAAATTCTGACGCTCCATATACTTCTTTTGATGGTGAAGTTCCAACTCATGATGGCGAAGTTCCTACTTTTGGCAGCTCTGAAAGTTCCAGTTCATATGAATCATCTCCCACTGAAGAATCCAATGTGGATGAATCTATGGAATATCAGAGCTATGAAACATATTCAGAACTGTGA
Protein sequence

>MsG0180004658.01.T01

MGNEWGIDGGSEKMREKSGIFDSAEKCRELIGEEGSSQSGKFTLLNCFDMGSGTLACAVKEGVKLYFYSIRSTHVEKARQRAIESALVDAVSQGMPPTDAAKHAQKESKKAAKLASRQAKRIIGPIISSGWDFFEAIYYGGTITEGFLRGTGTLFGAYGGGFFGEQSLGRIGYLVGSHMGSWVGGRIGLMIYDVINGVHLLLEFVQTGEIEVRKTLVHENSDAPYTSFDGEVPTHDGEVPTFGSSESSSSYESSPTEESNVDESMEYQSYETYSEL*