AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010908.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010908.01.T01 MTR_2g094270 62.698 126 42 3 1 123 162 285 4.10e-39 132
MsG0280010908.01.T01 MTR_4g059390 56.800 125 49 3 2 123 161 283 6.10e-33 117
MsG0280010908.01.T01 MTR_7g101190 53.488 129 55 3 1 126 160 286 2.39e-32 115
MsG0280010908.01.T01 MTR_3g118010 51.200 125 56 3 2 123 153 275 2.90e-28 104
MsG0280010908.01.T01 MTR_1g095070 48.780 123 58 3 4 123 97 217 6.31e-27 99.8
MsG0280010908.01.T01 MTR_8g098375 42.373 118 63 3 2 116 169 284 5.23e-19 80.5
MsG0280010908.01.T01 MTR_5g010150 40.833 120 66 3 1 117 168 285 1.66e-18 79.0
MsG0280010908.01.T01 MTR_3g070210 40.336 119 66 3 2 117 172 288 6.92e-18 77.4
MsG0280010908.01.T01 MTR_5g082070 40.336 119 66 3 2 117 172 288 1.11e-17 77.0
MsG0280010908.01.T01 MTR_4g415300 39.167 120 68 3 1 117 171 288 2.55e-17 75.9
MsG0280010908.01.T02 MTR_2g094270 62.698 126 42 3 1 123 162 285 4.10e-39 132
MsG0280010908.01.T02 MTR_4g059390 56.800 125 49 3 2 123 161 283 6.10e-33 117
MsG0280010908.01.T02 MTR_7g101190 53.488 129 55 3 1 126 160 286 2.39e-32 115
MsG0280010908.01.T02 MTR_3g118010 51.200 125 56 3 2 123 153 275 2.90e-28 104
MsG0280010908.01.T02 MTR_1g095070 48.780 123 58 3 4 123 97 217 6.31e-27 99.8
MsG0280010908.01.T02 MTR_8g098375 42.373 118 63 3 2 116 169 284 5.23e-19 80.5
MsG0280010908.01.T02 MTR_5g010150 40.833 120 66 3 1 117 168 285 1.66e-18 79.0
MsG0280010908.01.T02 MTR_3g070210 40.336 119 66 3 2 117 172 288 6.92e-18 77.4
MsG0280010908.01.T02 MTR_5g082070 40.336 119 66 3 2 117 172 288 1.11e-17 77.0
MsG0280010908.01.T02 MTR_4g415300 39.167 120 68 3 1 117 171 288 2.55e-17 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010908.01.T01 AT4G35100 56.800 125 49 3 2 123 154 276 6.06e-33 117
MsG0280010908.01.T01 AT4G35100 56.800 125 49 3 2 123 154 276 6.06e-33 117
MsG0280010908.01.T01 AT2G16850 52.800 125 54 3 2 123 152 274 5.81e-30 108
MsG0280010908.01.T01 AT5G60660 52.000 125 55 3 2 123 161 283 1.89e-29 108
MsG0280010908.01.T01 AT2G39010 48.462 130 62 3 1 127 159 286 2.64e-29 107
MsG0280010908.01.T01 AT3G53420 46.875 128 63 3 2 126 161 286 2.64e-26 99.8
MsG0280010908.01.T01 AT3G53420 46.875 128 63 3 2 126 161 286 2.64e-26 99.8
MsG0280010908.01.T01 AT2G37180 46.094 128 64 3 2 126 159 284 6.89e-26 98.6
MsG0280010908.01.T01 AT2G37170 46.094 128 64 3 2 126 159 284 6.97e-26 98.6
MsG0280010908.01.T01 AT2G37170 46.094 128 64 3 2 126 202 327 1.04e-25 99.0
MsG0280010908.01.T01 AT3G54820 46.032 126 63 3 2 124 160 283 9.89e-25 95.5
MsG0280010908.01.T01 AT1G01620 41.176 119 65 3 2 117 96 212 1.52e-19 80.5
MsG0280010908.01.T01 AT4G23400 40.833 120 66 3 1 117 168 285 3.01e-19 81.3
MsG0280010908.01.T01 AT1G01620 41.176 119 65 3 2 117 168 284 3.97e-19 80.9
MsG0280010908.01.T01 AT4G00430 40.336 119 66 3 2 117 169 285 1.01e-18 79.7
MsG0280010908.01.T01 AT2G45960 39.496 119 67 3 2 117 168 284 3.10e-18 78.6
MsG0280010908.01.T01 AT3G61430 38.655 119 68 3 2 117 168 284 2.15e-17 76.3
MsG0280010908.01.T01 AT3G61430 38.655 119 68 3 2 117 168 284 2.15e-17 76.3
MsG0280010908.01.T02 AT4G35100 56.800 125 49 3 2 123 154 276 6.06e-33 117
MsG0280010908.01.T02 AT4G35100 56.800 125 49 3 2 123 154 276 6.06e-33 117
MsG0280010908.01.T02 AT2G16850 52.800 125 54 3 2 123 152 274 5.81e-30 108
MsG0280010908.01.T02 AT5G60660 52.000 125 55 3 2 123 161 283 1.89e-29 108
MsG0280010908.01.T02 AT2G39010 48.462 130 62 3 1 127 159 286 2.64e-29 107
MsG0280010908.01.T02 AT3G53420 46.875 128 63 3 2 126 161 286 2.64e-26 99.8
MsG0280010908.01.T02 AT3G53420 46.875 128 63 3 2 126 161 286 2.64e-26 99.8
MsG0280010908.01.T02 AT2G37180 46.094 128 64 3 2 126 159 284 6.89e-26 98.6
MsG0280010908.01.T02 AT2G37170 46.094 128 64 3 2 126 159 284 6.97e-26 98.6
MsG0280010908.01.T02 AT2G37170 46.094 128 64 3 2 126 202 327 1.04e-25 99.0
MsG0280010908.01.T02 AT3G54820 46.032 126 63 3 2 124 160 283 9.89e-25 95.5
MsG0280010908.01.T02 AT1G01620 41.176 119 65 3 2 117 96 212 1.52e-19 80.5
MsG0280010908.01.T02 AT4G23400 40.833 120 66 3 1 117 168 285 3.01e-19 81.3
MsG0280010908.01.T02 AT1G01620 41.176 119 65 3 2 117 168 284 3.97e-19 80.9
MsG0280010908.01.T02 AT4G00430 40.336 119 66 3 2 117 169 285 1.01e-18 79.7
MsG0280010908.01.T02 AT2G45960 39.496 119 67 3 2 117 168 284 3.10e-18 78.6
MsG0280010908.01.T02 AT3G61430 38.655 119 68 3 2 117 168 284 2.15e-17 76.3
MsG0280010908.01.T02 AT3G61430 38.655 119 68 3 2 117 168 284 2.15e-17 76.3

Find 33 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGGACCCCATGTAATTT+TGG 0.167779 2:+76642165 None:intergenic
ATGATACTGTTATTCTTAAA+AGG 0.198224 2:+76642292 None:intergenic
GGCCCTATGTGTTTGGAATT+TGG 0.200363 2:-76642202 MsG0280010908.01.T01:three_prime_UTR
TATTGGGTTGGACCATTTAT+TGG 0.240800 2:-76642449 MsG0280010908.01.T01:CDS
GCCACAGCAGCACCAATAAA+TGG 0.305067 2:+76642437 None:intergenic
CTTTAATTGCTGATCCTCTA+AGG 0.336597 2:+76642393 None:intergenic
AAATAAAGAAACAAGTAAAA+TGG 0.337630 2:+76641988 None:intergenic
TACCACCAATACATCCTTAG+AGG 0.364277 2:-76642407 MsG0280010908.01.T01:CDS
TTAAAGCTCTTGGATCCTTC+AGG 0.374939 2:-76642376 MsG0280010908.01.T01:CDS
TCAATTATTATCTATGTTGT+TGG 0.377852 2:-76642323 MsG0280010908.01.T01:three_prime_UTR
GCTTAATGAATGAATATGTT+TGG 0.418929 2:-76642348 MsG0280010908.01.T01:exon
GGATCAGCAATTAAAGCTCT+TGG 0.421288 2:-76642386 MsG0280010908.01.T01:CDS
ATTATCTATGTTGTTGGTGT+TGG 0.422606 2:-76642317 MsG0280010908.01.T01:three_prime_UTR
CTGATCCTCTAAGGATGTAT+TGG 0.458362 2:+76642402 None:intergenic
TCGTAATGGCCCTATGTGTT+TGG 0.461650 2:-76642209 MsG0280010908.01.T01:three_prime_UTR
TATGTGTTTGGAATTTGGAT+TGG 0.469993 2:-76642197 MsG0280010908.01.T01:three_prime_UTR
GTTCAGTGGATTTATTGGGT+TGG 0.486574 2:-76642461 MsG0280010908.01.T01:intron
CATATTGAGTAGTTGTCTAC+TGG 0.487507 2:-76642272 MsG0280010908.01.T01:intron
TTTCAATCCCATGTTCGTAA+TGG 0.492704 2:-76642223 MsG0280010908.01.T01:intron
ACCATTTATTGGTGCTGCTG+TGG 0.499187 2:-76642438 MsG0280010908.01.T01:CDS
AGTTGTCTACTGGTCCCTCA+TGG 0.512268 2:-76642262 MsG0280010908.01.T01:intron
ATCCTCTAAGGATGTATTGG+TGG 0.516154 2:+76642405 None:intergenic
TTCATTAAGCATGCTCCTGA+AGG 0.533039 2:+76642361 None:intergenic
AATCCAAATTCCAAACACAT+AGG 0.540210 2:+76642199 None:intergenic
ACATAGGGCCATTACGAACA+TGG 0.540754 2:+76642215 None:intergenic
ATAAAGAAGCCAAAATTACA+TGG 0.553497 2:-76642174 MsG0280010908.01.T01:three_prime_UTR
ATCCAAATTCCAAACACATA+GGG 0.562751 2:+76642200 None:intergenic
TAAAGAAGCCAAAATTACAT+GGG 0.586175 2:-76642173 MsG0280010908.01.T01:three_prime_UTR
AACATGGGATTGAAAACAAG+AGG 0.610197 2:+76642231 None:intergenic
CATAGGGCCATTACGAACAT+GGG 0.613681 2:+76642216 None:intergenic
CAAGAGGACATGACTCCATG+AGG 0.657538 2:+76642247 None:intergenic
AAAGAAGCCAAAATTACATG+GGG 0.678318 2:-76642172 MsG0280010908.01.T01:three_prime_UTR
AAGAGGACATGACTCCATGA+GGG 0.685063 2:+76642248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATTGAAATTAATTAAT+TGG - Chr2:76642926-76642945 MsG0280010908.01.T01:intron 10.0%
!!! TTTGAAATCATTAATTAAAT+AGG - Chr2:76643355-76643374 MsG0280010908.01.T01:intron 10.0%
!! AAATAAAGAAACAAGTAAAA+TGG + Chr2:76644230-76644249 None:intergenic 15.0%
!! AACTAAAAATAAAAAAGAGA+AGG + Chr2:76644123-76644142 None:intergenic 15.0%
!! ATCATTAATTAAATAGGTAT+TGG - Chr2:76643361-76643380 MsG0280010908.01.T01:intron 15.0%
!! TCAATTATTAGCATATATAA+AGG + Chr2:76643700-76643719 None:intergenic 15.0%
!!! ATCAAATTAATCACTTTTTT+GGG - Chr2:76643269-76643288 MsG0280010908.01.T01:intron 15.0%
!!! ATTTTTTGTTTAATAACATG+TGG - Chr2:76642554-76642573 MsG0280010908.01.T01:intron 15.0%
!!! TATCAAATTAATCACTTTTT+TGG - Chr2:76643268-76643287 MsG0280010908.01.T01:intron 15.0%
!!! TCTAAATTGTACTTATTTTT+TGG + Chr2:76642681-76642700 None:intergenic 15.0%
!!! TTTAAATAGAAAATTTTGAG+TGG + Chr2:76643661-76643680 None:intergenic 15.0%
!! AAAAATAAAAAAGAGAAGGA+AGG + Chr2:76644119-76644138 None:intergenic 20.0%
!! AAAATAAAAAAGAGAAGGAA+GGG + Chr2:76644118-76644137 None:intergenic 20.0%
!! ATGATACTGTTATTCTTAAA+AGG + Chr2:76643926-76643945 None:intergenic 20.0%
!! CCAAATTAACCAAAAAATTA+AGG + Chr2:76642453-76642472 None:intergenic 20.0%
!! TAATATTTCATAATTGTAGG+AGG - Chr2:76642949-76642968 MsG0280010908.01.T01:intron 20.0%
!! TCAATTATTATCTATGTTGT+TGG - Chr2:76643892-76643911 MsG0280010908.01.T01:intron 20.0%
!! TGAATATTGTTAGAATTAGT+TGG + Chr2:76642404-76642423 None:intergenic 20.0%
!! TGGTAATATTTCATAATTGT+AGG - Chr2:76642946-76642965 MsG0280010908.01.T01:intron 20.0%
!! TGTATCATATACACTTTATA+AGG - Chr2:76642352-76642371 MsG0280010908.01.T01:exon 20.0%
!!! AACGATTTACCTTAATTTTT+TGG - Chr2:76642441-76642460 MsG0280010908.01.T01:CDS 20.0%
!!! CAGTTAATTTTCTTTTTAAG+TGG + Chr2:76642797-76642816 None:intergenic 20.0%
!!! CCTTAATTTTTTGGTTAATT+TGG - Chr2:76642450-76642469 MsG0280010908.01.T01:CDS 20.0%
!!! TAGGTATTTTTTTCTTTTGA+GGG - Chr2:76642887-76642906 MsG0280010908.01.T01:intron 20.0%
! ATAAAGAAGCCAAAATTACA+TGG - Chr2:76644041-76644060 MsG0280010908.01.T01:intron 25.0%
! GCTTAATGAATGAATATGTT+TGG - Chr2:76643867-76643886 MsG0280010908.01.T01:intron 25.0%
! TAAAGAAGCCAAAATTACAT+GGG - Chr2:76644042-76644061 MsG0280010908.01.T01:intron 25.0%
! TTACAAGTTAGTAATGTAGT+CGG - Chr2:76642325-76642344 MsG0280010908.01.T01:three_prime_UTR 25.0%
! TTGTCTACTATAACCAATAA+TGG + Chr2:76642162-76642181 None:intergenic 25.0%
!! ATAAGCATGCTTATGTTTTA+AGG - Chr2:76644081-76644100 MsG0280010908.01.T01:intron 25.0%
!! GTGATTAATTTGATACTTAC+AGG + Chr2:76643263-76643282 None:intergenic 25.0%
!!! AAATAGAAAATTTTGAGTGG+TGG + Chr2:76643658-76643677 None:intergenic 25.0%
!!! AAATTATGGTACCTTTGTTT+TGG - Chr2:76643181-76643200 MsG0280010908.01.T01:five_prime_UTR 25.0%
!!! AGGTATTTTTTTCTTTTGAG+GGG - Chr2:76642888-76642907 MsG0280010908.01.T01:intron 25.0%
!!! GTAGGTATTTTTTTCTTTTG+AGG - Chr2:76642886-76642905 MsG0280010908.01.T01:intron 25.0%
!!! TAAGCATGCTTATGTTTTAA+GGG - Chr2:76644082-76644101 MsG0280010908.01.T01:intron 25.0%
!!! TTTTGTTCAGTGGATTTATT+GGG - Chr2:76643750-76643769 MsG0280010908.01.T01:intron 25.0%
!!! TTTTTGTTCAGTGGATTTAT+TGG - Chr2:76643749-76643768 MsG0280010908.01.T01:intron 25.0%
AAACTCATGTGTATCAAACA+AGG + Chr2:76642065-76642084 None:intergenic 30.0%
AAAGAAGCCAAAATTACATG+GGG - Chr2:76644043-76644062 MsG0280010908.01.T01:intron 30.0%
AATCCAAATTCCAAACACAT+AGG + Chr2:76644019-76644038 None:intergenic 30.0%
ACAAAGTGATACACTATTGA+TGG - Chr2:76642178-76642197 MsG0280010908.01.T01:three_prime_UTR 30.0%
ATCCAAATTCCAAACACATA+GGG + Chr2:76644018-76644037 None:intergenic 30.0%
ATTATCTATGTTGTTGGTGT+TGG - Chr2:76643898-76643917 MsG0280010908.01.T01:intron 30.0%
ATTTGATACTTACAGGAACA+TGG + Chr2:76643256-76643275 None:intergenic 30.0%
CAAGGTTACATAAGTCAAAA+TGG - Chr2:76642834-76642853 MsG0280010908.01.T01:CDS 30.0%
CTCGATATTGTAGAGAATTA+CGG - Chr2:76643540-76643559 MsG0280010908.01.T01:intron 30.0%
GAAAAAAATGTGCAATTGCA+AGG - Chr2:76642816-76642835 MsG0280010908.01.T01:CDS 30.0%
GGTGGTATTTAGTATATATG+TGG + Chr2:76643640-76643659 None:intergenic 30.0%
TTTCTCGTTGTTGAAGATAA+CGG + Chr2:76643481-76643500 None:intergenic 30.0%
TTTGATACTTACAGGAACAT+GGG + Chr2:76643255-76643274 None:intergenic 30.0%
! CATGCTTATCTACAATTTTG+AGG + Chr2:76644070-76644089 None:intergenic 30.0%
! GAATATCGTTTAGCTTAGTT+AGG + Chr2:76642382-76642401 None:intergenic 30.0%
! TATGTGTTTGGAATTTGGAT+TGG - Chr2:76644018-76644037 MsG0280010908.01.T01:intron 30.0%
!!! ATCACTGTTTTGACCATTAT+TGG - Chr2:76642146-76642165 MsG0280010908.01.T01:three_prime_UTR 30.0%
!!! TGTTTGTTGTTTTTGTTCAG+TGG - Chr2:76643740-76643759 MsG0280010908.01.T01:intron 30.0%
AAAAGCAAACACACAATCTC+TGG + Chr2:76642756-76642775 None:intergenic 35.0%
AAACTTGTGTTGTCTTGTGT+AGG - Chr2:76642868-76642887 MsG0280010908.01.T01:intron 35.0%
AACACTGTGCAACTATGTAT+AGG + Chr2:76643041-76643060 None:intergenic 35.0%
AACAGTGTAGACCAAAACAA+AGG + Chr2:76643195-76643214 None:intergenic 35.0%
AACATGGGATTGAAAACAAG+AGG + Chr2:76643987-76644006 None:intergenic 35.0%
AACTACCTTAACAGAGAGAA+CGG + Chr2:76642307-76642326 None:intergenic 35.0%
ACCTGTTGTAGTATGATGTT+TGG + Chr2:76643101-76643120 None:intergenic 35.0%
ATGGTCAAAACAGTGATGTA+AGG + Chr2:76642143-76642162 None:intergenic 35.0%
CATATTGAGTAGTTGTCTAC+TGG - Chr2:76643943-76643962 MsG0280010908.01.T01:intron 35.0%
CTTTAATTGCTGATCCTCTA+AGG + Chr2:76643825-76643844 None:intergenic 35.0%
GATACACATGAGTTTCAAAG+TGG - Chr2:76642070-76642089 MsG0280010908.01.T01:three_prime_UTR 35.0%
TACATAGTTGCACAGTGTTT+AGG - Chr2:76643042-76643061 MsG0280010908.01.T01:CDS 35.0%
TCATACTACAACAGGTACAA+AGG - Chr2:76643105-76643124 MsG0280010908.01.T01:five_prime_UTR 35.0%
TCCAAACATCATACTACAAC+AGG - Chr2:76643097-76643116 MsG0280010908.01.T01:intron 35.0%
TTTCAATCCCATGTTCGTAA+TGG - Chr2:76643992-76644011 MsG0280010908.01.T01:intron 35.0%
! ACGCAAGGTGTCTTTAATAA+GGG - Chr2:76643011-76643030 MsG0280010908.01.T01:CDS 35.0%
!! TATTGGGTTGGACCATTTAT+TGG - Chr2:76643766-76643785 MsG0280010908.01.T01:intron 35.0%
!! TGCTTTAGGTGCTGAAATTA+TGG - Chr2:76643167-76643186 MsG0280010908.01.T01:five_prime_UTR 35.0%
ATGTGTATCAAACAAGGTGC+TGG + Chr2:76642059-76642078 None:intergenic 40.0%
CAGTGTTTAGGAGCAATTTG+TGG - Chr2:76643054-76643073 MsG0280010908.01.T01:CDS 40.0%
CATGAACACAGCAAATCCAT+GGG + Chr2:76643395-76643414 None:intergenic 40.0%
GATGGTAACACTGAATGTGA+TGG - Chr2:76642196-76642215 MsG0280010908.01.T01:three_prime_UTR 40.0%
GATGTTGAGGTTCAAGAACA+AGG - Chr2:76641996-76642015 MsG0280010908.01.T01:three_prime_UTR 40.0%
GGAGTTAACATGGTTTCTGA+TGG - Chr2:76643129-76643148 MsG0280010908.01.T01:five_prime_UTR 40.0%
GGATCAGCAATTAAAGCTCT+TGG - Chr2:76643829-76643848 MsG0280010908.01.T01:intron 40.0%
GTACAAAGGAGGAGTTAACA+TGG - Chr2:76643119-76643138 MsG0280010908.01.T01:five_prime_UTR 40.0%
GTGACCTTTGGACTATTAGT+GGG - Chr2:76642988-76643007 MsG0280010908.01.T01:CDS 40.0%
GTTTCTGATGGTTACAGCAA+AGG - Chr2:76643141-76643160 MsG0280010908.01.T01:five_prime_UTR 40.0%
TACCACCAATACATCCTTAG+AGG - Chr2:76643808-76643827 MsG0280010908.01.T01:intron 40.0%
TACTACAACAGGTACAAAGG+AGG - Chr2:76643108-76643127 MsG0280010908.01.T01:five_prime_UTR 40.0%
TGTAGGAGGACACATTAATC+CGG - Chr2:76642963-76642982 MsG0280010908.01.T01:intron 40.0%
TGTGACCTTTGGACTATTAG+TGG - Chr2:76642987-76643006 MsG0280010908.01.T01:CDS 40.0%
TTAAAGCTCTTGGATCCTTC+AGG - Chr2:76643839-76643858 MsG0280010908.01.T01:intron 40.0%
TTCATTAAGCATGCTCCTGA+AGG + Chr2:76643857-76643876 None:intergenic 40.0%
! AACACTGAATGTGATGGTGT+TGG - Chr2:76642202-76642221 MsG0280010908.01.T01:three_prime_UTR 40.0%
! ATCCTCTAAGGATGTATTGG+TGG + Chr2:76643813-76643832 None:intergenic 40.0%
! CTGATCCTCTAAGGATGTAT+TGG + Chr2:76643816-76643835 None:intergenic 40.0%
! GACGCAAGGTGTCTTTAATA+AGG - Chr2:76643010-76643029 MsG0280010908.01.T01:CDS 40.0%
! GTTCAGTGGATTTATTGGGT+TGG - Chr2:76643754-76643773 MsG0280010908.01.T01:intron 40.0%
! TTGAGGACCCCATGTAATTT+TGG + Chr2:76644053-76644072 None:intergenic 40.0%
!! AGGTGCTGAATTTACAGCTA+AGG - Chr2:76642016-76642035 MsG0280010908.01.T01:three_prime_UTR 40.0%
!! TGATCCTAAGAGAAGTGCTA+GGG - Chr2:76643226-76643245 MsG0280010908.01.T01:five_prime_UTR 40.0%
!!! ATGTGATGGTGTTGGTGTTT+TGG - Chr2:76642210-76642229 MsG0280010908.01.T01:three_prime_UTR 40.0%
!!! TGGTGTTTTGGGAATTGCTT+GGG - Chr2:76642222-76642241 MsG0280010908.01.T01:intron 40.0%
!!! TGTGATGGTGTTGGTGTTTT+GGG - Chr2:76642211-76642230 MsG0280010908.01.T01:three_prime_UTR 40.0%
!!! TTGGTGTTTTGGGAATTGCT+TGG - Chr2:76642221-76642240 MsG0280010908.01.T01:intron 40.0%
AAGAGGACATGACTCCATGA+GGG + Chr2:76643970-76643989 None:intergenic 45.0%
AATAGTCCAAAGGTCACAGC+CGG + Chr2:76642985-76643004 None:intergenic 45.0%
ACATAGGGCCATTACGAACA+TGG + Chr2:76644003-76644022 None:intergenic 45.0%
AGCTACCGTTCTCTCTGTTA+AGG - Chr2:76642299-76642318 MsG0280010908.01.T01:three_prime_UTR 45.0%
ATTAATCCGGCTGTGACCTT+TGG - Chr2:76642976-76642995 MsG0280010908.01.T01:CDS 45.0%
CATAGGGCCATTACGAACAT+GGG + Chr2:76644002-76644021 None:intergenic 45.0%
CCATGAACACAGCAAATCCA+TGG + Chr2:76643396-76643415 None:intergenic 45.0%
CTTCAACAACGAGAAAGCCT+GGG - Chr2:76643485-76643504 MsG0280010908.01.T01:intron 45.0%
GAATCCCTAGCACTTCTCTT+AGG + Chr2:76643233-76643252 None:intergenic 45.0%
GACCAGGTAAATTCAGTGTC+AGG - Chr2:76643510-76643529 MsG0280010908.01.T01:intron 45.0%
TATTACCCTGCCAGGAGTTT+CGG - Chr2:76643450-76643469 MsG0280010908.01.T01:intron 45.0%
TCGTAATGGCCCTATGTGTT+TGG - Chr2:76644006-76644025 MsG0280010908.01.T01:intron 45.0%
TCTTCAACAACGAGAAAGCC+TGG - Chr2:76643484-76643503 MsG0280010908.01.T01:intron 45.0%
TGAATTTACCTGGTCATCCC+AGG + Chr2:76643505-76643524 None:intergenic 45.0%
TGCCTGACACTGAATTTACC+TGG + Chr2:76643515-76643534 None:intergenic 45.0%
TGCTGTGTTCATGGTTCACT+TGG - Chr2:76643402-76643421 MsG0280010908.01.T01:intron 45.0%
TTAGGAGCAATTTGTGGTGC+TGG - Chr2:76643060-76643079 MsG0280010908.01.T01:CDS 45.0%
! CAGTTGATGCGATCACATTG+TGG - Chr2:76643570-76643589 MsG0280010908.01.T01:intron 45.0%
! GGCCCTATGTGTTTGGAATT+TGG - Chr2:76644013-76644032 MsG0280010908.01.T01:intron 45.0%
! TCGTTGTTGAAGATAACGGC+TGG + Chr2:76643477-76643496 None:intergenic 45.0%
! TTGGGAATTGCTTGGGCTTT+TGG - Chr2:76642229-76642248 MsG0280010908.01.T01:intron 45.0%
!! ACCATTTATTGGTGCTGCTG+TGG - Chr2:76643777-76643796 MsG0280010908.01.T01:intron 45.0%
!! CCATGGATTTGCTGTGTTCA+TGG - Chr2:76643393-76643412 MsG0280010908.01.T01:intron 45.0%
!! CTGATCCTAAGAGAAGTGCT+AGG - Chr2:76643225-76643244 MsG0280010908.01.T01:five_prime_UTR 45.0%
!! TACAGCAAAGGCACTGCTTT+AGG - Chr2:76643153-76643172 MsG0280010908.01.T01:five_prime_UTR 45.0%
!! TGTATCAAACAAGGTGCTGG+TGG + Chr2:76642056-76642075 None:intergenic 45.0%
AACTCCTGGCAGGGTAATAC+CGG + Chr2:76643449-76643468 None:intergenic 50.0%
AGTTGTCTACTGGTCCCTCA+TGG - Chr2:76643953-76643972 MsG0280010908.01.T01:intron 50.0%
CAAGAGGACATGACTCCATG+AGG + Chr2:76643971-76643990 None:intergenic 50.0%
CACAGCAAATCCATGGGAAG+TGG + Chr2:76643389-76643408 None:intergenic 50.0%
CGGTAGCTTACCAGAGATAC+CGG + Chr2:76642287-76642306 None:intergenic 50.0%
GCCACAGCAGCACCAATAAA+TGG + Chr2:76643781-76643800 None:intergenic 50.0%
GCGTCCCACTAATAGTCCAA+AGG + Chr2:76642995-76643014 None:intergenic 50.0%
TAGCTTACCAGAGATACCGG+CGG + Chr2:76642284-76642303 None:intergenic 50.0%
TGGACTATTAGTGGGACGCA+AGG - Chr2:76642996-76643015 MsG0280010908.01.T01:CDS 50.0%
! ATCCTTGTTTACTGCACCGC+CGG - Chr2:76642265-76642284 MsG0280010908.01.T01:intron 50.0%
! GGAATTGCTTGGGCTTTTGG+TGG - Chr2:76642232-76642251 MsG0280010908.01.T01:intron 50.0%
! TGTGGTGCTGGACTTGCAAA+GGG - Chr2:76643072-76643091 MsG0280010908.01.T01:CDS 50.0%
! TTGTGGTGCTGGACTTGCAA+AGG - Chr2:76643071-76643090 MsG0280010908.01.T01:CDS 50.0%
!! ATCAAACAAGGTGCTGGTGG+TGG + Chr2:76642053-76642072 None:intergenic 50.0%
!! GGTAATACCGGTTCCGGTAA+CGG + Chr2:76643437-76643456 None:intergenic 50.0%
!! GTAATACCGGTTCCGGTAAC+GGG + Chr2:76643436-76643455 None:intergenic 50.0%
!! TAATACCGGTTCCGGTAACG+GGG + Chr2:76643435-76643454 None:intergenic 50.0%
TACCGGCGGTGCAGTAAACA+AGG + Chr2:76642270-76642289 None:intergenic 55.0%
TTGGCTACCATCCCCGTTAC+CGG - Chr2:76643421-76643440 MsG0280010908.01.T01:intron 55.0%
!! AAGGTGCTGGTGGTGGATCT+TGG + Chr2:76642046-76642065 None:intergenic 55.0%
!! GGTATTGGCACCACTTCCCA+TGG - Chr2:76643376-76643395 MsG0280010908.01.T01:intron 55.0%
!! TGGCAGGGTAATACCGGTTC+CGG + Chr2:76643443-76643462 None:intergenic 55.0%
ACCATCCCCGTTACCGGAAC+CGG - Chr2:76643427-76643446 MsG0280010908.01.T01:intron 60.0%
CGAGAAAGCCTGGGATGACC+AGG - Chr2:76643494-76643513 MsG0280010908.01.T01:intron 60.0%
CTGGTCCGAAACTCCTGGCA+GGG + Chr2:76643458-76643477 None:intergenic 60.0%
GGAACCGGTATTACCCTGCC+AGG - Chr2:76643442-76643461 MsG0280010908.01.T01:intron 60.0%
TACTGCACCGCCGGTATCTC+TGG - Chr2:76642274-76642293 MsG0280010908.01.T01:three_prime_UTR 60.0%
! AACGGCTGGTCCGAAACTCC+TGG + Chr2:76643463-76643482 None:intergenic 60.0%
GCTGGTCCGAAACTCCTGGC+AGG + Chr2:76643459-76643478 None:intergenic 65.0%
!! ACCGGTTCCGGTAACGGGGA+TGG + Chr2:76643431-76643450 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 76641987 76644250 76641987 ID=MsG0280010908.01;Name=MsG0280010908.01
Chr2 mRNA 76641987 76644250 76641987 ID=MsG0280010908.01.T01;Parent=MsG0280010908.01;Name=MsG0280010908.01.T01;_AED=0.49;_eAED=0.55;_QI=291|0.75|0.8|1|0.75|0.6|5|338|127
Chr2 exon 76641987 76642239 76641987 ID=MsG0280010908.01.T01:exon:11298;Parent=MsG0280010908.01.T01
Chr2 exon 76642273 76642477 76642273 ID=MsG0280010908.01.T01:exon:11297;Parent=MsG0280010908.01.T01
Chr2 five_prime_UTR 76644130 76644250 76644130 ID=MsG0280010908.01.T01:five_prime_utr;Parent=MsG0280010908.01.T01
Chr2 five_prime_UTR 76643100 76643269 76643100 ID=MsG0280010908.01.T01:five_prime_utr;Parent=MsG0280010908.01.T01
Chr2 CDS 76642975 76643099 76642975 ID=MsG0280010908.01.T01:cds;Parent=MsG0280010908.01.T01
Chr2 CDS 76642722 76642860 76642722 ID=MsG0280010908.01.T01:cds;Parent=MsG0280010908.01.T01
Chr2 CDS 76642358 76642477 76642358 ID=MsG0280010908.01.T01:cds;Parent=MsG0280010908.01.T01
Chr2 three_prime_UTR 76642273 76642357 76642273 ID=MsG0280010908.01.T01:three_prime_utr;Parent=MsG0280010908.01.T01
Chr2 three_prime_UTR 76641987 76642239 76641987 ID=MsG0280010908.01.T01:three_prime_utr;Parent=MsG0280010908.01.T01
Chr2 mRNA 76641991 76644250 76641991 ID=MsG0280010908.01.T02;Parent=MsG0280010908.01;Name=MsG0280010908.01.T02;_AED=0.49;_eAED=0.55;_QI=291|0.66|0.75|1|1|1|4|367|127
Chr2 exon 76641991 76642477 76641991 ID=MsG0280010908.01.T02:exon:11299;Parent=MsG0280010908.01.T02
Chr2 five_prime_UTR 76644130 76644250 76644130 ID=MsG0280010908.01.T02:five_prime_utr;Parent=MsG0280010908.01.T02
Chr2 five_prime_UTR 76643100 76643269 76643100 ID=MsG0280010908.01.T02:five_prime_utr;Parent=MsG0280010908.01.T02
Chr2 CDS 76642975 76643099 76642975 ID=MsG0280010908.01.T02:cds;Parent=MsG0280010908.01.T02
Chr2 CDS 76642722 76642860 76642722 ID=MsG0280010908.01.T02:cds;Parent=MsG0280010908.01.T02
Chr2 CDS 76642358 76642477 76642358 ID=MsG0280010908.01.T02:cds;Parent=MsG0280010908.01.T02
Chr2 three_prime_UTR 76641991 76642357 76641991 ID=MsG0280010908.01.T02:three_prime_utr;Parent=MsG0280010908.01.T02
Gene Sequence

>MsG0280010908.01.T01

ATGGTTTCTGATGGTTACAGCAAAGGCACTGCTTTAGGTGCTGAAATTATGGTACCTTTGTTTTGGTCTACACTGTTTTCTCTGCTACTGATCCTAAGAGAAGTGCTAGGGATTCCCATGTTCCTGTATTGGCACCACTTCCCATGGATTTGCTGTGTTCATGGTTCACTTGGCTACCATCCCCGTTACCGGAACCGGTATTACCCTGCCAGGAGTTTCGGACCAGCCGTTATCTTCAACAACGAGAAAGCCTGGGATGACCAGTGGATTTATTGGGTTGGACCATTTATTGGTGCTGCTGTGGCTGCAATTTACCACCAATACATCCTTAGAGGATCAGCAATTAAAGCTCTTGGATCCTTCAGGAGCATGCTTAATGAATGA

>MsG0280010908.01.T02

ATGGTTTCTGATGGTTACAGCAAAGGCACTGCTTTAGGTGCTGAAATTATGGTACCTTTGTTTTGGTCTACACTGTTTTCTCTGCTACTGATCCTAAGAGAAGTGCTAGGGATTCCCATGTTCCTGTATTGGCACCACTTCCCATGGATTTGCTGTGTTCATGGTTCACTTGGCTACCATCCCCGTTACCGGAACCGGTATTACCCTGCCAGGAGTTTCGGACCAGCCGTTATCTTCAACAACGAGAAAGCCTGGGATGACCAGTGGATTTATTGGGTTGGACCATTTATTGGTGCTGCTGTGGCTGCAATTTACCACCAATACATCCTTAGAGGATCAGCAATTAAAGCTCTTGGATCCTTCAGGAGCATGCTTAATGAATGA
Protein sequence

>MsG0280010908.01.T01

MVSDGYSKGTALGAEIMVPLFWSTLFSLLLILREVLGIPMFLYWHHFPWICCVHGSLGYHPRYRNRYYPARSFGPAVIFNNEKAWDDQWIYWVGPFIGAAVAAIYHQYILRGSAIKALGSFRSMLNE*

>MsG0280010908.01.T02

MVSDGYSKGTALGAEIMVPLFWSTLFSLLLILREVLGIPMFLYWHHFPWICCVHGSLGYHPRYRNRYYPARSFGPAVIFNNEKAWDDQWIYWVGPFIGAAVAAIYHQYILRGSAIKALGSFRSMLNE*