AlfalfaGEDB | MsG0180006064.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006064.01

KEGG: K02147
GO: GO:0005524
PFAM: PF00006

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006064.01.T01	XP_013470345.1	74.468	188	39	4	1	185	1	182	5.05E-77	249
MsG0180006064.01.T02	ABO61030.1	77.333	300	23	2	4	303	235	489	1.95E-154	451

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006064.01.T01	Q43432	69.149	188	49	4	1	185	1	182	4.97E-73	231
MsG0180006064.01.T02	Q40079	76.846	298	24	2	4	301	229	481	5.87E-155	444

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006064.01.T01	A0A072VSL4	74.468	188	39	4	1	185	1	182	2.41e-77	249
MsG0180006064.01.T02	A0A2P5WGU4	75.806	310	60	3	4	303	268	572	1.12e-154	454
MsG0180006064.01.T02	A6Y950	77.333	300	23	2	4	303	235	489	9.33e-155	451

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048683.01	MsG0180006064.01	-0.830813	2.377349e-55	2.532526e-52
MsG0080048764.01	MsG0180006064.01	-0.804947	1.691322e-49	8.836352e-47
MsG0080049148.01	MsG0180006064.01	-0.803342	3.649388e-49	1.829303e-46
MsG0180000240.01	MsG0180006064.01	0.908337	1.909518e-81	3.529000e-77
MsG0180000470.01	MsG0180006064.01	-0.838290	3.139729e-57	4.185941e-54
MsG0180000550.01	MsG0180006064.01	-0.807290	5.432023e-50	3.014014e-47
MsG0180000650.01	MsG0180006064.01	-0.836141	1.113303e-56	1.390622e-53
MsG0180000828.01	MsG0180006064.01	-0.832338	1.001021e-55	1.115404e-52
MsG0180001712.01	MsG0180006064.01	-0.804297	2.311853e-49	1.187675e-46
MsG0180002977.01	MsG0180006064.01	0.822046	2.920231e-53	2.416917e-50

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006064.01.T01	MTR_1g112870	74.468	188	39	4	1	185	1	182	6.11e-81	249
MsG0180006064.01.T01	MTR_7g081010	72.340	188	42	5	1	185	1	181	4.89e-76	236
MsG0180006064.01.T02	MTR_1g112870	77.333	300	23	2	4	303	235	489	2.70e-158	451
MsG0180006064.01.T02	MTR_7g081010	76.333	300	26	2	4	303	233	487	4.50e-156	445

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006064.01.T01	AT4G38510	75.000	168	35	4	1	168	8	168	2.43e-72	227
MsG0180006064.01.T01	AT4G38510	75.000	168	35	4	1	168	1	161	3.03e-72	226
MsG0180006064.01.T01	AT4G38510	75.000	168	35	4	1	168	1	161	3.03e-72	226
MsG0180006064.01.T01	AT4G38510	75.000	168	35	4	1	168	1	161	3.03e-72	226
MsG0180006064.01.T01	AT4G38510	75.000	168	35	4	1	168	1	161	3.03e-72	226
MsG0180006064.01.T01	AT1G20260	74.850	167	34	4	3	168	1	160	5.89e-72	225
MsG0180006064.01.T01	AT1G76030	76.829	164	30	4	6	168	4	160	7.69e-72	225
MsG0180006064.01.T02	AT4G38510	76.333	300	26	2	4	303	233	487	7.54e-156	444
MsG0180006064.01.T02	AT4G38510	76.333	300	26	2	4	303	233	487	7.54e-156	444
MsG0180006064.01.T02	AT4G38510	76.333	300	26	2	4	303	233	487	7.54e-156	444

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Find 93 sgRNAs with CRISPR-Local

Find 421 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTAATCCAACATGGTTTA+TGG	0.208291	1:-99604031	None:intergenic
AAGCGTGACTTTGAGGAGAA+CGG	0.558460	1:+99602736	MsG0180006064.01.T01:three_prime_UTR
AAGTAAGCCTCAGGTAAAAT+TGG	0.379673	1:-99601248	None:intergenic
AATGCTAGCGACAATGACGA+CGG	0.701949	1:+99599530	MsG0180006064.01.T01:CDS
ACAGAATACAGGACTGTGTC+TGG	0.446233	1:+99599802	MsG0180006064.01.T01:intron
ACCTCGAGAACTTGTCCACG+CGG	0.733370	1:-99600011	None:intergenic
ACGTACCATTCCTATCTCCA+AGG	0.645021	1:-99599555	None:intergenic
ACGTCATGAATTCTATTGCT+AGG	0.469761	1:+99601439	MsG0180006064.01.T01:CDS
ACTATTGATCAGGTATTTGA+AGG	0.479209	1:+99600321	MsG0180006064.01.T01:CDS
ACTTTCTCAAGAATGACCAA+TGG	0.606143	1:-99599835	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTAATCCAACATGGTTTA+TGG	-	Chr1:99604034-99604053	None:intergenic	30.0%
ACACTCTAATGATGTATCAT+TGG	+	Chr1:99604535-99604554	MsG0180006064.01.T01:intron	30.0%
ACCAAAATTACCAGAATAAG+GGG	-	Chr1:99602834-99602853	None:intergenic	30.0%
ACGAACAGATAAATACATCA+AGG	-	Chr1:99605493-99605512	None:intergenic	30.0%
ACTATTGATCAGGTATTTGA+AGG	+	Chr1:99600321-99600340	MsG0180006064.01.T01:CDS	30.0%
AGAGAAAATTAGTAGATACC+AGG	-	Chr1:99604825-99604844	None:intergenic	30.0%
AGTTTCTACATAAGAGTTGT+CGG	-	Chr1:99602464-99602483	None:intergenic	30.0%
CAATCTCATACAATTCATCA+GGG	-	Chr1:99604315-99604334	None:intergenic	30.0%
CACAAATATAGTTGTCAAAG+CGG	+	Chr1:99601840-99601859	MsG0180006064.01.T01:intron	30.0%
CATGGTTAGAATTGGAATTT+GGG	-	Chr1:99603790-99603809	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	99599515	99605690	99599515	ID=MsG0180006064.01;Name=MsG0180006064.01
Chr1	mRNA	99599515	99605690	99599515	ID=MsG0180006064.01.T01;Parent=MsG0180006064.01;Name=MsG0180006064.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|0.76\|0.71\|1\|0.61\|0.64\|14\|1491\|191
Chr1	exon	99599515	99599575	99599515	ID=MsG0180006064.01.T01:exon:12611;Parent=MsG0180006064.01.T01
Chr1	exon	99599806	99599874	99599806	ID=MsG0180006064.01.T01:exon:12612;Parent=MsG0180006064.01.T01
Chr1	exon	99599961	99600031	99599961	ID=MsG0180006064.01.T01:exon:12613;Parent=MsG0180006064.01.T01
Chr1	exon	99600303	99600392	99600303	ID=MsG0180006064.01.T01:exon:12614;Parent=MsG0180006064.01.T01
Chr1	exon	99601170	99601281	99601170	ID=MsG0180006064.01.T01:exon:12615;Parent=MsG0180006064.01.T01
Chr1	exon	99601370	99601577	99601370	ID=MsG0180006064.01.T01:exon:12616;Parent=MsG0180006064.01.T01
Chr1	exon	99602661	99602792	99602661	ID=MsG0180006064.01.T01:exon:12617;Parent=MsG0180006064.01.T01
Chr1	exon	99603370	99603506	99603370	ID=MsG0180006064.01.T01:exon:12618;Parent=MsG0180006064.01.T01
Chr1	exon	99603914	99603995	99603914	ID=MsG0180006064.01.T01:exon:12620;Parent=MsG0180006064.01.T01
Chr1	exon	99604410	99604457	99604410	ID=MsG0180006064.01.T01:exon:12621;Parent=MsG0180006064.01.T01
Chr1	CDS	99599515	99599575	99599515	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	CDS	99599806	99599874	99599806	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	CDS	99599961	99600031	99599961	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	CDS	99600303	99600392	99600303	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	CDS	99601170	99601281	99601170	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	CDS	99601370	99601542	99601370	ID=MsG0180006064.01.T01:cds;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99601543	99601577	99601543	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99602661	99602792	99602661	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99603370	99603506	99603370	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99603671	99603819	99603671	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99603914	99603995	99603914	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99604410	99604457	99604410	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99604570	99604623	99604570	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99604742	99604825	99604742	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	three_prime_UTR	99604921	99605690	99604921	ID=MsG0180006064.01.T01:three_prime_utr;Parent=MsG0180006064.01.T01
Chr1	mRNA	99603336	99605690	99603336	ID=MsG0180006064.01.T02;Parent=MsG0180006064.01;Name=MsG0180006064.01.T02;_AED=0.41;_eAED=0.42;_QI=0\|0.66\|0.57\|1\|0.83\|0.85\|7\|584\|303
Chr1	exon	99603336	99603520	99603336	ID=MsG0180006064.01.T02:exon:12625;Parent=MsG0180006064.01.T02
Chr1	exon	99603914	99604083	99603914	ID=MsG0180006064.01.T02:exon:12626;Parent=MsG0180006064.01.T02
Chr1	exon	99604374	99604457	99604374	ID=MsG0180006064.01.T02:exon:12627;Parent=MsG0180006064.01.T02
Chr1	CDS	99603336	99603520	99603336	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99603671	99603819	99603671	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99603914	99604083	99603914	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99604374	99604457	99604374	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99604570	99604623	99604570	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99604742	99604825	99604742	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	CDS	99604921	99605106	99604921	ID=MsG0180006064.01.T02:cds;Parent=MsG0180006064.01.T02
Chr1	three_prime_UTR	99605107	99605690	99605107	ID=MsG0180006064.01.T02:three_prime_utr;Parent=MsG0180006064.01.T02

Gene Sequence

>MsG0180006064.01.T02

ATGTCTGTACAATTGCTATTGGGATTTACTGTAGGCAATGATCCTACAATTGAACGTATTATCACTCCTCGTATTGCTCTTACTACTGCTGAATATCTGGCGTATGAATGTGGCAAGCATGTTCTCGTGATACTCACAGATATGAGTTCTTATGCTGATGCTCTTCGTGAGGTAATGTACCAACTGTATCTGCTGCCAGAGAAGAGGTGCCAGGAAGACGTGGTTATCCTGGTTATGTATACAGATCTTGCAACAATTTATGAACGTGCCGGAAGAATTGAGGGGCGTAAAGGCTCAATTACCCAAATTCCAATTCTAACCATGCCAAATGATGATATTACACATCCAACTCCTGATCTTACCGGATATATTACGGAGGGGCAGATATACATCGATAGGCAGTTGCATAATAACAGGTGGGTCATGTTGAAGCTTTCTGCTGTGATGAATGCCATAAACCATGTTGGATTAGTTGCTGCATTATTATGTGAAATTTGGTGCAGACATTATTCTTTTTTTTTTCTTTTTTTTCTCGAGCAGATATACCCACCAATCAATGTTCTTCCATCACTATCTCGGTTGATGAAGAGTGCTATTGGGGAGGGAATGACTAGAAAGGATCATTCTGATGTGTCCAACCAGCTGTATGCAAATTATGCCATTGGAAAGGATGTCCAGGCAATGAAAGCTGTTGTTGGAGAAGAAGCACTTTCATCTGAGGACCTGTTGTATCTGGAATTTCTGGACAAGTTTGAGAGGAAGTTTGTTGCTCAGGGAGCCTACGACACCCGCAATATATTCCAGTCTCTAGATCTTGCATGGACCTTACTCCGGATTTTCCCCCGTGAACTTCTTCATCGTATACCAGCCAAGACTCTTGACCAGTTTTACAGCCGAGATGCTGGTAATTGA

>MsG0180006064.01.T01

ATGGGTGTGGCTCAGAATGCTAGCGACAATGACGACGGCACCTTGGAGATAGGAATGGTACAATACAGGACTGTGTCTGGAGTTGCCGGACCATTGGTCATTCTTGAGAAAGTGAAGGTAACGACTGACAGGACCCAAATATCAAGAGATTGTCAATATTCGTTTAGGCGATGGAACTACCCGCGTGGACAAGTTCTCGAGTTTACTGACTTTATTAAAACTATTGATCAGGTATTTGAAGGTACATCTGGGATTGACAATAAATTTACGACCGTACAGTTTACTGGAGAGGTGTTAAAAACTCCTGTATCACTGGACATGCTTGGTCGGATATTTAACGGTTCTGGAAAACCAATTGACAATGGTCCGCCAATTTTACCTGAGGCTTACTTGGATATATCAGGAAGTTCCATCAACCCTAGTGAGAGAACCTATCCCGAGGAGATGATACAGACTGGAATATCTACAATTGACGTCATGAATTCTATTGCTAGGGTCAAAAGATCCCTCTATTTTCAGCTGCCGGTCTCCCCCATAATGAAATTGCTGCACAGATTTGTCGTCAAGCTGGCTTAG

Protein sequence

>MsG0180006064.01.T02

MSVQLLLGFTVGNDPTIERIITPRIALTTAEYLAYECGKHVLVILTDMSSYADALREVMYQLYLLPEKRCQEDVVILVMYTDLATIYERAGRIEGRKGSITQIPILTMPNDDITHPTPDLTGYITEGQIYIDRQLHNNRWVMLKLSAVMNAINHVGLVAALLCEIWCRHYSFFFLFFLEQIYPPINVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYANYAIGKDVQAMKAVVGEEALSSEDLLYLEFLDKFERKFVAQGAYDTRNIFQSLDLAWTLLRIFPRELLHRIPAKTLDQFYSRDAGN*

>MsG0180006064.01.T01

MGVAQNASDNDDGTLEIGMVQYRTVSGVAGPLVILEKVKVTTDRTQISRDCQYSFRRWNYPRGQVLEFTDFIKTIDQVFEGTSGIDNKFTTVQFTGEVLKTPVSLDMLGRIFNGSGKPIDNGPPILPEAYLDISGSSINPSERTYPEEMIQTGISTIDVMNSIARVKRSLYFQLPVSPIMKLLHRFVVKLA*