AlfalfaGEDB | MsG0280011024.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011024.01

KEGG: -
GO: -
PFAM: PF14309

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	KEH39432.1	95.833	72	3	0	1	72	659	730	4.19E-37	141

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	F4HSD5	50.685	73	33	2	1	70	477	549	3.10E-12	62.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	A0A072VCE5	95.833	72	3	0	1	72	659	730	2.00e-37	141

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048205.01	MsG0280011024.01	0.827774	1.299590e-54	1.266862e-51
MsG0080048207.01	MsG0280011024.01	0.816942	4.262918e-52	3.062881e-49
MsG0080048915.01	MsG0280011024.01	0.998890	1.182761e-280	5.360490e-273
MsG0180000235.01	MsG0280011024.01	0.801523	8.651408e-49	4.140483e-46
MsG0180000266.01	MsG0280011024.01	0.820224	7.679611e-53	6.040529e-50
MsG0180000267.01	MsG0280011024.01	0.822377	2.447549e-53	2.045189e-50
MsG0180000585.01	MsG0280011024.01	0.813324	2.710989e-51	1.764936e-48
MsG0180000822.01	MsG0280011024.01	0.806246	9.029135e-50	4.877034e-47
MsG0180001663.01	MsG0280011024.01	0.808837	2.545767e-50	1.470798e-47
MsG0180001921.01	MsG0280011024.01	0.813131	2.989608e-51	1.935977e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	MTR_2g096870	95.833	72	3	1	72	659	730	5.07e-41	141
MsG0280011024.01.T01	MTR_2g096870	95.833	72	3	1	72	621	692	5.43e-41	141
MsG0280011024.01.T01	MTR_1g047660	44.928	69	38	4	72	779	847	3.87e-14	65.5
MsG0280011024.01.T01	MTR_4g021865	44.118	68	38	4	71	361	428	3.06e-13	62.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	503	575	3.08e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT5G02390	46.032	63	33	1	1	62	762	824	1.03e-12	61.6
MsG0280011024.01.T01	AT5G02390	46.032	63	33	1	1	62	762	824	1.03e-12	61.6

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Find 13 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCAAACCAATGCCTAA+AGG	0.488700	2:+78564327	None:intergenic
AACTCAAACCAATGCCTAAA+GGG	0.384062	2:+78564328	None:intergenic
ACTATCTTGTTAAAGAAGTA+TGG	0.258917	2:+78564352	MsG0280011024.01.T01:CDS
AGATAGTTCCCTTTAGGCAT+TGG	0.454693	2:-78564336	None:intergenic
ATTTGACGAAAAGAAGTGGT+TGG	0.594040	2:+78564445	MsG0280011024.01.T01:CDS
CAGACATTAGATGATGTTGT+TGG	0.474147	2:+78564417	MsG0280011024.01.T01:CDS
CGTGATTTGACGAAAAGAAG+TGG	0.655367	2:+78564441	MsG0280011024.01.T01:CDS
CTATCTTGTTAAAGAAGTAT+GGG	0.540514	2:+78564353	MsG0280011024.01.T01:CDS
GATGAATCTTCAACAGGAAG+AGG	0.528776	2:+78564467	MsG0280011024.01.T01:CDS
GTGTGTAGCACTTGAACTTG+AGG	0.590237	2:+78564491	MsG0280011024.01.T01:CDS

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAACAAGATAGTTCCCTTT+AGG	-	Chr2:78564345-78564364	None:intergenic	30.0%
	ATTTGACGAAAAGAAGTGGT+TGG	+	Chr2:78564445-78564464	MsG0280011024.01.T01:CDS	35.0%
	CAGACATTAGATGATGTTGT+TGG	+	Chr2:78564417-78564436	MsG0280011024.01.T01:CDS	35.0%
	TCTAATGTCTGATCTAACTC+TGG	-	Chr2:78564408-78564427	None:intergenic	35.0%
	CGTGATTTGACGAAAAGAAG+TGG	+	Chr2:78564441-78564460	MsG0280011024.01.T01:CDS	40.0%
	GATGAATCTTCAACAGGAAG+AGG	+	Chr2:78564467-78564486	MsG0280011024.01.T01:CDS	40.0%
	TGGTTGGATGAATCTTCAAC+AGG	+	Chr2:78564461-78564480	MsG0280011024.01.T01:CDS	40.0%
	GTGTGTAGCACTTGAACTTG+AGG	+	Chr2:78564491-78564510	MsG0280011024.01.T01:CDS	45.0%
!	ACTATCTTGTTAAAGAAGTA+TGG	+	Chr2:78564352-78564371	MsG0280011024.01.T01:CDS	25.0%
!!	CTATCTTGTTAAAGAAGTAT+GGG	+	Chr2:78564353-78564372	MsG0280011024.01.T01:CDS	25.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	78564339	78564557	78564339	ID=MsG0280011024.01;Name=MsG0280011024.01
Chr2	mRNA	78564339	78564557	78564339	ID=MsG0280011024.01.T01;Parent=MsG0280011024.01;Name=MsG0280011024.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|72
Chr2	exon	78564339	78564557	78564339	ID=MsG0280011024.01.T01:exon:37578;Parent=MsG0280011024.01.T01
Chr2	CDS	78564339	78564557	78564339	ID=MsG0280011024.01.T01:cds;Parent=MsG0280011024.01.T01

Gene Sequence	>MsG0280011024.01.T01 ATGCCTAAAGGGAACTATCTTGTTAAAGAAGTATGGGACAGTGTTAGTTCATATTTGATGTTGAGACCAGAGTTAGATCAGACATTAGATGATGTTGTTGGTCGTGATTTGACGAAAAGAAGTGGTTGGATGAATCTTCAACAGGAAGAGGAGTGTGTAGCACTTGAACTTGAGGAAATGATCATAGATGATTTATTAGATGAAATTCTCTTCTCATGA
Protein sequence	>MsG0280011024.01.T01 MPKGNYLVKEVWDSVSSYLMLRPELDQTLDDVVGRDLTKRSGWMNLQQEEECVALELEEMIIDDLLDEILFS*