AlfalfaGEDB | MsG0280011024.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011024.01

KEGG: -
GO: -
PFAM: PF14309

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	KEH39432.1	95.833	72	3	0	1	72	659	730	4.19E-37	141

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	F4HSD5	50.685	73	33	2	1	70	477	549	3.10E-12	62.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	A0A072VCE5	95.833	72	3	0	1	72	659	730	2.00e-37	141

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048205.01	MsG0280011024.01	0.827774	1.299590e-54	1.266862e-51
MsG0080048207.01	MsG0280011024.01	0.816942	4.262918e-52	3.062881e-49
MsG0080048915.01	MsG0280011024.01	0.998890	1.182761e-280	5.360490e-273
MsG0180000235.01	MsG0280011024.01	0.801523	8.651408e-49	4.140483e-46
MsG0180000266.01	MsG0280011024.01	0.820224	7.679611e-53	6.040529e-50
MsG0180000267.01	MsG0280011024.01	0.822377	2.447549e-53	2.045189e-50
MsG0180000585.01	MsG0280011024.01	0.813324	2.710989e-51	1.764936e-48
MsG0180000822.01	MsG0280011024.01	0.806246	9.029135e-50	4.877034e-47
MsG0180001663.01	MsG0280011024.01	0.808837	2.545767e-50	1.470798e-47
MsG0180001921.01	MsG0280011024.01	0.813131	2.989608e-51	1.935977e-48
MsG0180002912.01	MsG0280011024.01	0.828945	6.780786e-55	6.839536e-52
MsG0180004005.01	MsG0280011024.01	0.805218	1.484621e-49	7.810046e-47
MsG0180004060.01	MsG0280011024.01	0.812887	3.382054e-51	2.175911e-48
MsG0180004388.01	MsG0280011024.01	0.816749	4.709742e-52	3.365818e-49
MsG0180004762.01	MsG0280011024.01	0.812093	5.041735e-51	3.175885e-48
MsG0180005172.01	MsG0280011024.01	0.800629	1.317746e-48	6.165609e-46
MsG0180005842.01	MsG0280011024.01	0.800076	1.707444e-48	7.877644e-46
MsG0180005944.01	MsG0280011024.01	0.818552	1.846546e-52	1.386584e-49
MsG0180006016.01	MsG0280011024.01	0.813751	2.184312e-51	1.438569e-48
MsG0280006356.01	MsG0280011024.01	0.822683	2.077853e-53	1.751381e-50
MsG0280010614.01	MsG0280011024.01	0.829352	5.400950e-55	5.514583e-52
MsG0280010739.01	MsG0280011024.01	0.823794	1.143479e-53	9.946089e-51
MsG0280010784.01	MsG0280011024.01	0.818260	2.151334e-52	1.602374e-49
MsG0280011024.01	MsG0280011052.01	0.823490	1.346736e-53	1.161331e-50
MsG0280011024.01	MsG0280011131.01	0.815751	7.872867e-52	5.475068e-49
MsG0280011024.01	MsG0280011446.01	0.841424	4.792919e-58	7.039878e-55
MsG0280011024.01	MsG0380011663.01	0.814878	1.231163e-51	8.359208e-49
MsG0280011024.01	MsG0380011754.01	0.840513	8.309154e-58	1.186389e-54
MsG0280011024.01	MsG0380012020.01	0.801434	9.022968e-49	4.308547e-46
MsG0280011024.01	MsG0380012033.01	0.802616	5.155210e-49	2.536781e-46
MsG0280011024.01	MsG0380013047.01	0.829649	4.573666e-55	4.710197e-52
MsG0280011024.01	MsG0380014573.01	0.833127	6.373714e-56	7.273033e-53
MsG0280011024.01	MsG0380014680.01	0.809256	2.069762e-50	1.209352e-47
MsG0280011024.01	MsG0380015181.01	0.808126	3.610087e-50	2.047291e-47
MsG0280011024.01	MsG0380015324.01	0.811628	6.364597e-51	3.959363e-48
MsG0280011024.01	MsG0380015877.01	0.843834	1.098124e-58	1.739643e-55
MsG0280011024.01	MsG0380015931.01	0.822682	2.079270e-53	1.752532e-50
MsG0280011024.01	MsG0380015986.01	0.837903	3.948562e-57	5.203068e-54
MsG0280011024.01	MsG0380016227.01	0.812594	3.920230e-51	2.502806e-48
MsG0280011024.01	MsG0380016581.01	0.802978	4.339495e-49	2.155074e-46
MsG0280011024.01	MsG0380016624.01	0.802948	4.402927e-49	2.184910e-46
MsG0280011024.01	MsG0380016859.01	0.801043	1.084784e-48	5.129468e-46
MsG0280011024.01	MsG0380017140.01	0.831430	1.676814e-55	1.819129e-52
MsG0280011024.01	MsG0380017158.01	0.815096	1.101208e-51	7.521040e-49
MsG0280011024.01	MsG0380017286.01	0.827937	1.186946e-54	1.162682e-51
MsG0280011024.01	MsG0380017403.01	0.814545	1.458564e-51	9.815455e-49
MsG0280011024.01	MsG0380017631.01	0.807182	5.726577e-50	3.168528e-47
MsG0280011024.01	MsG0380017879.01	0.814091	1.837727e-51	1.221524e-48
MsG0280011024.01	MsG0380017940.01	0.809079	2.258816e-50	1.313577e-47
MsG0280011024.01	MsG0380018009.01	0.827764	1.306214e-54	1.273011e-51
MsG0280011024.01	MsG0480018635.01	0.807802	4.231568e-50	2.379321e-47
MsG0280011024.01	MsG0480018970.01	0.817419	3.329482e-52	2.423562e-49
MsG0280011024.01	MsG0480020445.01	0.840247	9.753249e-58	1.381133e-54
MsG0280011024.01	MsG0480020886.01	0.821357	4.214378e-53	3.420873e-50
MsG0280011024.01	MsG0480021021.01	0.811244	7.714075e-51	4.750588e-48
MsG0280011024.01	MsG0480022451.01	0.803485	3.409158e-49	1.715274e-46
MsG0280011024.01	MsG0480022480.01	0.823449	1.376703e-53	1.185706e-50
MsG0280011024.01	MsG0480023224.01	0.831649	1.480960e-55	1.617197e-52
MsG0280011024.01	MsG0480023245.01	0.815992	6.957484e-52	4.870209e-49
MsG0280011024.01	MsG0480023248.01	0.807088	5.995771e-50	3.309199e-47
MsG0280011024.01	MsG0480023427.01	0.808261	3.378806e-50	1.923039e-47
MsG0280011024.01	MsG0480023517.01	0.814902	1.216202e-51	8.262971e-49
MsG0280011024.01	MsG0480023554.01	0.813377	2.640335e-51	1.721448e-48
MsG0280011024.01	MsG0580024114.01	0.825751	3.950742e-54	3.633428e-51
MsG0280011024.01	MsG0580024204.01	0.800232	1.587646e-48	7.353861e-46
MsG0280011024.01	MsG0580024205.01	0.819442	1.158732e-52	8.917936e-50
MsG0280011024.01	MsG0580024305.01	0.810266	1.255511e-50	7.533752e-48
MsG0280011024.01	MsG0580024306.01	0.811282	7.570578e-51	4.666699e-48
MsG0280011024.01	MsG0580024682.01	0.807036	6.150262e-50	3.389975e-47
MsG0280011024.01	MsG0580024853.01	0.802762	4.811119e-49	2.376310e-46
MsG0280011024.01	MsG0580025328.01	0.805064	1.598834e-49	8.377864e-47
MsG0280011024.01	MsG0580025907.01	0.809601	1.745292e-50	1.029231e-47
MsG0280011024.01	MsG0580026596.01	0.818119	2.314180e-52	1.717111e-49
MsG0280011024.01	MsG0580027129.01	0.845591	3.690775e-59	6.180649e-56
MsG0280011024.01	MsG0580027228.01	0.824734	6.875551e-54	6.142707e-51
MsG0280011024.01	MsG0580027345.01	0.824566	7.530874e-54	6.695694e-51
MsG0280011024.01	MsG0580027532.01	0.814692	1.353662e-51	9.145345e-49
MsG0280011024.01	MsG0580027847.01	0.826868	2.142001e-54	2.033644e-51
MsG0280011024.01	MsG0580028527.01	0.807913	4.005905e-50	2.258903e-47
MsG0280011024.01	MsG0580028969.01	0.830520	2.804307e-55	2.961537e-52
MsG0280011024.01	MsG0580029554.01	0.802048	6.749397e-49	3.273491e-46
MsG0280011024.01	MsG0580029758.01	0.800216	1.599411e-48	7.405426e-46
MsG0280011024.01	MsG0580030235.01	0.815987	6.975722e-52	4.882307e-49
MsG0280011024.01	MsG0680030629.01	0.813379	2.636897e-51	1.719325e-48
MsG0280011024.01	MsG0680030685.01	0.822370	2.457089e-53	2.052750e-50
MsG0280011024.01	MsG0680031719.01	0.803762	2.985764e-49	1.512984e-46
MsG0280011024.01	MsG0680034036.01	0.803785	2.953767e-49	1.497657e-46
MsG0280011024.01	MsG0680034450.01	0.835169	1.962130e-56	2.380103e-53
MsG0280011024.01	MsG0680035460.01	0.814906	1.213354e-51	8.244559e-49
MsG0280011024.01	MsG0680035573.01	0.803313	3.700777e-49	1.853682e-46
MsG0280011024.01	MsG0680035656.01	0.813141	2.974263e-51	1.926563e-48
MsG0280011024.01	MsG0680035844.01	0.830269	3.230050e-55	3.386520e-52
MsG0280011024.01	MsG0780035958.01	0.816436	5.534771e-52	3.921474e-49
MsG0280011024.01	MsG0780036548.01	0.829285	5.608569e-55	5.715608e-52
MsG0280011024.01	MsG0780036630.01	0.811508	6.760933e-51	4.192926e-48
MsG0280011024.01	MsG0780037515.01	0.814698	1.349098e-51	9.116121e-49
MsG0280011024.01	MsG0780038117.01	0.823677	1.217793e-53	1.055786e-50
MsG0280011024.01	MsG0780038323.01	0.801919	7.174011e-49	3.468000e-46
MsG0280011024.01	MsG0780038829.01	0.819414	1.176014e-52	9.043713e-50
MsG0280011024.01	MsG0780038921.01	0.807904	4.024964e-50	2.269038e-47
MsG0280011024.01	MsG0780039875.01	0.819546	1.097639e-52	8.472146e-50
MsG0280011024.01	MsG0780040442.01	0.818363	2.038710e-52	1.522982e-49
MsG0280011024.01	MsG0780040505.01	0.822922	1.827584e-53	1.550907e-50
MsG0280011024.01	MsG0780040516.01	0.832963	7.002497e-56	7.950475e-53
MsG0280011024.01	MsG0780040679.01	0.825283	5.101220e-54	4.629309e-51
MsG0280011024.01	MsG0780040726.01	0.812495	4.119626e-51	2.623339e-48
MsG0280011024.01	MsG0780040758.01	0.803189	3.924841e-49	1.959797e-46
MsG0280011024.01	MsG0780040951.01	0.816204	6.236696e-52	4.391248e-49
MsG0280011024.01	MsG0780041161.01	0.833876	4.145529e-56	4.837606e-53
MsG0280011024.01	MsG0780041368.01	0.809193	2.135830e-50	1.245861e-47
MsG0280011024.01	MsG0780041485.01	0.834417	3.033703e-56	3.598106e-53
MsG0280011024.01	MsG0780041777.01	0.810924	9.047906e-51	5.525650e-48
MsG0280011024.01	MsG0880042611.01	0.816003	6.918689e-52	4.844435e-49
MsG0280011024.01	MsG0880042843.01	0.837297	5.649970e-57	7.306806e-54
MsG0280011024.01	MsG0880042967.01	0.840077	1.080580e-57	1.522204e-54
MsG0280011024.01	MsG0880043001.01	0.812395	4.332360e-51	2.751564e-48
MsG0280011024.01	MsG0880043287.01	0.803238	3.835656e-49	1.917572e-46
MsG0280011024.01	MsG0880043489.01	0.809789	1.590876e-50	9.428195e-48
MsG0280011024.01	MsG0880043804.01	0.800009	1.762230e-48	8.116425e-46
MsG0280011024.01	MsG0880044346.01	0.809691	1.669997e-50	9.871297e-48
MsG0280011024.01	MsG0880045106.01	0.838252	3.211604e-57	4.276776e-54
MsG0280011024.01	MsG0880045518.01	0.808878	2.494045e-50	1.442522e-47
MsG0280011024.01	MsG0880046244.01	0.843560	1.299657e-58	2.041343e-55
MsG0280011024.01	MsG0880046329.01	0.845433	4.073219e-59	6.786759e-56
MsG0280011024.01	MsG0880046586.01	0.808046	3.754889e-50	2.124833e-47
MsG0280011024.01	MsG0880046642.01	0.829804	4.193309e-55	4.338583e-52
MsG0280011024.01	MsG0880047019.01	0.827287	1.700540e-54	1.634551e-51
MsG0280011024.01	MsG0880047143.01	0.813950	1.974649e-51	1.307511e-48
MsG0280011024.01	MsG0880047425.01	0.812522	4.064953e-51	2.590373e-48
MsG0280011024.01	MsG0880047497.01	0.837057	6.504373e-57	8.351105e-54
MsG0280007481.01	MsG0280011024.01	0.829057	6.369809e-55	6.446078e-52
MsG0280007585.01	MsG0280011024.01	0.805491	1.300923e-49	6.892019e-47
MsG0280008039.01	MsG0280011024.01	0.830317	3.143322e-55	3.300074e-52
MsG0280008073.01	MsG0280011024.01	0.819178	1.331437e-52	1.017176e-49
MsG0280008220.01	MsG0280011024.01	0.824412	8.186666e-54	7.245432e-51
MsG0280008270.01	MsG0280011024.01	0.805610	1.228599e-49	6.528600e-47
MsG0280008295.01	MsG0280011024.01	0.838021	3.683607e-57	4.871961e-54
MsG0280008907.01	MsG0280011024.01	0.804642	1.958469e-49	1.015119e-46
MsG0280008970.01	MsG0280011024.01	0.805033	1.622749e-49	8.496184e-47
MsG0280008971.01	MsG0280011024.01	0.806211	9.184773e-50	4.956662e-47
MsG0280009024.01	MsG0280011024.01	0.830321	3.136574e-55	3.293402e-52
MsG0280009610.01	MsG0280011024.01	0.804798	1.817325e-49	9.457943e-47
MsG0280009642.01	MsG0280011024.01	0.810209	1.291364e-50	7.737064e-48
MsG0280010416.01	MsG0280011024.01	0.809741	1.628421e-50	9.638923e-48
MsG0280010459.01	MsG0280011024.01	0.817954	2.522393e-52	1.863064e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	MTR_2g096870	95.833	72	3	1	72	659	730	5.07e-41	141
MsG0280011024.01.T01	MTR_2g096870	95.833	72	3	1	72	621	692	5.43e-41	141
MsG0280011024.01.T01	MTR_1g047660	44.928	69	38	4	72	779	847	3.87e-14	65.5
MsG0280011024.01.T01	MTR_4g021865	44.118	68	38	4	71	361	428	3.06e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	503	575	3.08e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT1G07620	50.685	73	33	2	1	70	477	549	3.16e-13	62.8
MsG0280011024.01.T01	AT5G02390	46.032	63	33	1	1	62	762	824	1.03e-12	61.6
MsG0280011024.01.T01	AT5G02390	46.032	63	33	1	1	62	762	824	1.03e-12	61.6

Find 13 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTATCTTGTTAAAGAAGTA+TGG	0.258917	2:+78564352	MsG0280011024.01.T01:CDS
TTAACAAGATAGTTCCCTTT+AGG	0.319585	2:-78564342	None:intergenic
AACTCAAACCAATGCCTAAA+GGG	0.384062	2:+78564328	None:intergenic
AGATAGTTCCCTTTAGGCAT+TGG	0.454693	2:-78564336	None:intergenic
CAGACATTAGATGATGTTGT+TGG	0.474147	2:+78564417	MsG0280011024.01.T01:CDS
AAACTCAAACCAATGCCTAA+AGG	0.488700	2:+78564327	None:intergenic
TGGTTGGATGAATCTTCAAC+AGG	0.489385	2:+78564461	MsG0280011024.01.T01:CDS
GATGAATCTTCAACAGGAAG+AGG	0.528776	2:+78564467	MsG0280011024.01.T01:CDS
CTATCTTGTTAAAGAAGTAT+GGG	0.540514	2:+78564353	MsG0280011024.01.T01:CDS
TCTAATGTCTGATCTAACTC+TGG	0.557841	2:-78564405	None:intergenic
GTGTGTAGCACTTGAACTTG+AGG	0.590237	2:+78564491	MsG0280011024.01.T01:CDS
ATTTGACGAAAAGAAGTGGT+TGG	0.594040	2:+78564445	MsG0280011024.01.T01:CDS
CGTGATTTGACGAAAAGAAG+TGG	0.655367	2:+78564441	MsG0280011024.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTATCTTGTTAAAGAAGTA+TGG	+	Chr2:78564352-78564371	MsG0280011024.01.T01:CDS	25.0%
!!	CTATCTTGTTAAAGAAGTAT+GGG	+	Chr2:78564353-78564372	MsG0280011024.01.T01:CDS	25.0%
	TTAACAAGATAGTTCCCTTT+AGG	-	Chr2:78564345-78564364	None:intergenic	30.0%
	ATTTGACGAAAAGAAGTGGT+TGG	+	Chr2:78564445-78564464	MsG0280011024.01.T01:CDS	35.0%
	CAGACATTAGATGATGTTGT+TGG	+	Chr2:78564417-78564436	MsG0280011024.01.T01:CDS	35.0%
	TCTAATGTCTGATCTAACTC+TGG	-	Chr2:78564408-78564427	None:intergenic	35.0%
	CGTGATTTGACGAAAAGAAG+TGG	+	Chr2:78564441-78564460	MsG0280011024.01.T01:CDS	40.0%
	GATGAATCTTCAACAGGAAG+AGG	+	Chr2:78564467-78564486	MsG0280011024.01.T01:CDS	40.0%
	TGGTTGGATGAATCTTCAAC+AGG	+	Chr2:78564461-78564480	MsG0280011024.01.T01:CDS	40.0%
	GTGTGTAGCACTTGAACTTG+AGG	+	Chr2:78564491-78564510	MsG0280011024.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	78564339	78564557	78564339	ID=MsG0280011024.01;Name=MsG0280011024.01
Chr2	mRNA	78564339	78564557	78564339	ID=MsG0280011024.01.T01;Parent=MsG0280011024.01;Name=MsG0280011024.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|72
Chr2	exon	78564339	78564557	78564339	ID=MsG0280011024.01.T01:exon:37578;Parent=MsG0280011024.01.T01
Chr2	CDS	78564339	78564557	78564339	ID=MsG0280011024.01.T01:cds;Parent=MsG0280011024.01.T01

Gene Sequence	>MsG0280011024.01.T01 ATGCCTAAAGGGAACTATCTTGTTAAAGAAGTATGGGACAGTGTTAGTTCATATTTGATGTTGAGACCAGAGTTAGATCAGACATTAGATGATGTTGTTGGTCGTGATTTGACGAAAAGAAGTGGTTGGATGAATCTTCAACAGGAAGAGGAGTGTGTAGCACTTGAACTTGAGGAAATGATCATAGATGATTTATTAGATGAAATTCTCTTCTCATGA
Protein sequence	>MsG0280011024.01.T01 MPKGNYLVKEVWDSVSSYLMLRPELDQTLDDVVGRDLTKRSGWMNLQQEEECVALELEEMIIDDLLDEILFS*