AlfalfaGEDB | MsG0180000267.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000267.01

KEGG: -
GO: -
PFAM: PF05701

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000267.01.T01	XP_039688711.1	98.413	63	1	0	1	63	792	854	2.89E-32	127

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000267.01.T01	O48724	66.667	54	18	0	1	54	680	733	8.29E-17	75.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000267.01.T01	G7I3K8	98.413	63	1	0	1	63	789	851	1.42e-32	127

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047825.01	MsG0180000267.01	0.821452	4.007160e-53	3.261285e-50
MsG0080048035.01	MsG0180000267.01	0.808105	3.646865e-50	2.066978e-47
MsG0080048874.01	MsG0180000267.01	0.800078	1.705988e-48	7.871323e-46
MsG0080048915.01	MsG0180000267.01	0.822672	2.090314e-53	1.761321e-50
MsG0180000266.01	MsG0180000267.01	0.825434	4.697166e-54	4.280633e-51
MsG0180000267.01	MsG0180000585.01	0.810779	9.729418e-51	5.918458e-48
MsG0180000267.01	MsG0180000669.01	0.802154	6.420094e-49	3.122174e-46
MsG0180000267.01	MsG0180000673.01	0.831415	1.691254e-55	1.833996e-52
MsG0180000267.01	MsG0180001663.01	0.822383	2.439222e-53	2.038590e-50
MsG0180000267.01	MsG0180001840.01	0.815600	8.508029e-52	5.892239e-49
MsG0180000267.01	MsG0180001921.01	0.827936	1.187874e-54	1.163544e-51
MsG0180000267.01	MsG0180002005.01	0.801861	7.374560e-49	3.559819e-46
MsG0180000267.01	MsG0180002912.01	0.852812	3.611642e-61	7.640892e-58
MsG0180000267.01	MsG0180004116.01	0.810138	1.338135e-50	8.002647e-48
MsG0180000267.01	MsG0180004388.01	0.828841	7.185749e-55	7.225686e-52
MsG0180000267.01	MsG0180004762.01	0.801009	1.101935e-48	5.206208e-46
MsG0180000267.01	MsG0180005125.01	0.806608	7.572217e-50	4.127814e-47
MsG0180000267.01	MsG0180005172.01	0.811606	6.435048e-51	4.000895e-48
MsG0180000267.01	MsG0180006016.01	0.816889	4.382630e-52	3.143952e-49
MsG0180000267.01	MsG0280006356.01	0.828459	8.884786e-55	8.836944e-52
MsG0180000267.01	MsG0280007272.01	0.804283	2.327467e-49	1.195246e-46
MsG0180000267.01	MsG0280007481.01	0.808479	3.035339e-50	1.737440e-47
MsG0180000267.01	MsG0280007826.01	0.807647	4.564884e-50	2.556286e-47
MsG0180000267.01	MsG0280008073.01	0.812576	3.955056e-51	2.523862e-48
MsG0180000267.01	MsG0280008220.01	0.839749	1.315662e-57	1.834184e-54
MsG0180000267.01	MsG0280008270.01	0.801620	8.264206e-49	3.964673e-46
MsG0180000267.01	MsG0280008295.01	0.809083	2.254376e-50	1.311146e-47
MsG0180000267.01	MsG0280008907.01	0.810562	1.083493e-50	6.552796e-48
MsG0180000267.01	MsG0280008971.01	0.815771	7.794566e-52	5.423628e-49
MsG0180000267.01	MsG0280009642.01	0.842569	2.387603e-58	3.635015e-55
MsG0180000267.01	MsG0280010389.01	0.804956	1.684007e-49	8.800171e-47
MsG0180000267.01	MsG0280010416.01	0.801409	9.129172e-49	4.356461e-46
MsG0180000267.01	MsG0280010443.01	0.801676	8.047192e-49	3.866117e-46
MsG0180000267.01	MsG0280010459.01	0.808351	3.232105e-50	1.843906e-47
MsG0180000267.01	MsG0280010614.01	0.824297	8.712295e-54	7.685206e-51
MsG0180000267.01	MsG0280010739.01	0.854119	1.521320e-61	3.362808e-58
MsG0180000267.01	MsG0280010784.01	0.814729	1.328514e-51	8.984464e-49
MsG0180000267.01	MsG0280011024.01	0.822377	2.447549e-53	2.045189e-50
MsG0180000267.01	MsG0280011070.01	0.800255	1.570407e-48	7.278327e-46
MsG0180000267.01	MsG0280011131.01	0.817620	3.000194e-52	2.195785e-49
MsG0180000267.01	MsG0280011235.01	0.819539	1.101122e-52	8.497699e-50
MsG0180000267.01	MsG0280011446.01	0.841705	4.040166e-58	5.985660e-55
MsG0180000267.01	MsG0380011754.01	0.863163	3.029464e-64	9.095864e-61
MsG0180000267.01	MsG0380012033.01	0.803655	3.143226e-49	1.588330e-46
MsG0180000267.01	MsG0380013047.01	0.832562	8.806679e-56	9.880130e-53
MsG0180000267.01	MsG0380013436.01	0.807154	5.807380e-50	3.210769e-47
MsG0180000267.01	MsG0380014135.01	0.803582	3.254757e-49	1.641769e-46
MsG0180000267.01	MsG0380014551.01	-0.802198	6.285214e-49	3.059908e-46
MsG0180000267.01	MsG0380014573.01	0.833927	4.025006e-56	4.704368e-53
MsG0180000267.01	MsG0380014770.01	0.804263	2.349718e-49	1.206031e-46
MsG0180000267.01	MsG0380015145.01	0.808984	2.367400e-50	1.373165e-47
MsG0180000267.01	MsG0380015181.01	0.803518	3.355595e-49	1.689775e-46
MsG0180000267.01	MsG0380015324.01	0.800233	1.586680e-48	7.349576e-46
MsG0180000267.01	MsG0380015779.01	0.802583	5.236343e-49	2.574515e-46
MsG0180000267.01	MsG0380015931.01	0.833536	5.042056e-56	5.823577e-53
MsG0180000267.01	MsG0380015986.01	0.819348	1.217361e-52	9.343546e-50
MsG0180000267.01	MsG0380016581.01	0.804409	2.189985e-49	1.128300e-46
MsG0180000267.01	MsG0380016859.01	0.800963	1.126119e-48	5.314151e-46
MsG0180000267.01	MsG0380017140.01	0.827367	1.626941e-54	1.567656e-51
MsG0180000267.01	MsG0380017226.01	0.813691	2.251569e-51	1.480506e-48
MsG0180000267.01	MsG0380017286.01	0.847613	1.034959e-59	1.849820e-56
MsG0180000267.01	MsG0380017356.01	-0.810294	1.238460e-50	7.436598e-48
MsG0180000267.01	MsG0380017403.01	0.817948	2.529694e-52	1.868160e-49
MsG0180000267.01	MsG0380017940.01	0.806444	8.203159e-50	4.453125e-47
MsG0180000267.01	MsG0380018009.01	0.831481	1.629479e-55	1.770562e-52
MsG0180000267.01	MsG0480018160.01	0.807985	3.867323e-50	2.185010e-47
MsG0180000267.01	MsG0480018635.01	0.849469	3.169066e-60	6.013527e-57
MsG0180000267.01	MsG0480018970.01	0.834836	2.379965e-56	2.858279e-53
MsG0180000267.01	MsG0480020445.01	0.851137	1.079523e-60	2.163793e-57
MsG0180000267.01	MsG0480020886.01	0.804021	2.637621e-49	1.345524e-46
MsG0180000267.01	MsG0480020964.01	0.801080	1.065626e-48	5.043587e-46
MsG0180000267.01	MsG0480021426.01	0.810863	9.327199e-51	5.686736e-48
MsG0180000267.01	MsG0480021853.01	0.803243	3.825693e-49	1.912872e-46
MsG0180000267.01	MsG0480022067.01	0.830637	2.624552e-55	2.781417e-52
MsG0180000267.01	MsG0480022274.01	0.801251	9.831808e-49	4.673349e-46
MsG0180000267.01	MsG0480022480.01	0.823318	1.477372e-53	1.267714e-50
MsG0180000267.01	MsG0480022551.01	0.800414	1.457436e-48	6.782314e-46
MsG0180000267.01	MsG0480023224.01	0.821133	4.748642e-53	3.830709e-50
MsG0180000267.01	MsG0480023245.01	0.802828	4.661921e-49	2.306641e-46
MsG0180000267.01	MsG0480023248.01	0.805608	1.229716e-49	6.534265e-47
MsG0180000267.01	MsG0480023427.01	0.825033	5.843620e-54	5.265712e-51
MsG0180000267.01	MsG0480023517.01	0.809658	1.697263e-50	1.002397e-47
MsG0180000267.01	MsG0480023654.01	0.803371	3.598605e-49	1.805229e-46
MsG0180000267.01	MsG0480023903.01	0.818149	2.279356e-52	1.692602e-49
MsG0180000267.01	MsG0580024073.01	0.818082	2.359935e-52	1.749139e-49
MsG0180000267.01	MsG0580024114.01	0.849328	3.470278e-60	6.556167e-57
MsG0180000267.01	MsG0580024472.01	0.826370	2.815138e-54	2.635155e-51
MsG0180000267.01	MsG0580025278.01	0.825501	4.529802e-54	4.136112e-51
MsG0180000267.01	MsG0580025328.01	0.809345	1.980893e-50	1.160214e-47
MsG0180000267.01	MsG0580025907.01	0.816227	6.165521e-52	4.343716e-49
MsG0180000267.01	MsG0580026595.01	0.800417	1.455237e-48	6.772599e-46
MsG0180000267.01	MsG0580027129.01	0.855926	4.543881e-62	1.066250e-58
MsG0180000267.01	MsG0580027345.01	0.822309	2.537894e-53	2.116468e-50
MsG0180000267.01	MsG0580027401.01	0.806855	6.715111e-50	3.684155e-47
MsG0180000267.01	MsG0580027773.01	0.810077	1.378754e-50	8.232647e-48
MsG0180000267.01	MsG0580027833.01	0.805028	1.626545e-49	8.514915e-47
MsG0180000267.01	MsG0580029189.01	0.804233	2.383832e-49	1.222574e-46
MsG0180000267.01	MsG0580029554.01	0.809508	1.827392e-50	1.074921e-47
MsG0180000267.01	MsG0580029691.01	0.821101	4.827891e-53	3.891329e-50
MsG0180000267.01	MsG0580029758.01	0.803757	2.993386e-49	1.516648e-46
MsG0180000267.01	MsG0580030235.01	0.807567	4.746384e-50	2.652440e-47
MsG0180000267.01	MsG0680030316.01	0.813747	2.188429e-51	1.441122e-48
MsG0180000267.01	MsG0680030629.01	0.821687	3.535834e-53	2.896878e-50
MsG0180000267.01	MsG0680030656.01	0.837470	5.099838e-57	6.631467e-54
MsG0180000267.01	MsG0680030685.01	0.826898	2.107153e-54	2.002463e-51
MsG0180000267.01	MsG0680031210.01	0.800370	1.487845e-48	6.916115e-46
MsG0180000267.01	MsG0680032641.01	0.804782	1.831066e-49	9.525687e-47
MsG0180000267.01	MsG0680034450.01	0.856329	3.463418e-62	8.236500e-59
MsG0180000267.01	MsG0680035656.01	0.813553	2.415049e-51	1.582085e-48
MsG0180000267.01	MsG0680035844.01	0.829367	5.356736e-55	5.471608e-52
MsG0180000267.01	MsG0780036177.01	-0.801296	9.627448e-49	4.581366e-46
MsG0180000267.01	MsG0780036291.01	0.817833	2.686829e-52	1.978024e-49
MsG0180000267.01	MsG0780037109.01	0.804242	2.373599e-49	1.217610e-46
MsG0180000267.01	MsG0780037515.01	0.826668	2.390936e-54	2.257029e-51
MsG0180000267.01	MsG0780038113.01	0.811993	5.302540e-51	3.331044e-48
MsG0180000267.01	MsG0780038117.01	0.800217	1.598325e-48	7.400668e-46
MsG0180000267.01	MsG0780038231.01	0.805067	1.596565e-49	8.366677e-47
MsG0180000267.01	MsG0780038444.01	0.823620	1.255750e-53	1.086861e-50
MsG0180000267.01	MsG0780038495.01	0.815342	9.709055e-52	6.676461e-49
MsG0180000267.01	MsG0780038829.01	0.815380	9.522407e-52	6.554848e-49
MsG0180000267.01	MsG0780039252.01	0.805886	1.074946e-49	5.752979e-47
MsG0180000267.01	MsG0780039875.01	0.800870	1.176799e-48	5.539952e-46
MsG0180000267.01	MsG0780039966.01	0.800317	1.525116e-48	7.079895e-46
MsG0180000267.01	MsG0780040442.01	0.836769	7.705128e-57	9.807797e-54
MsG0180000267.01	MsG0780040679.01	0.818712	1.698545e-52	1.281051e-49
MsG0180000267.01	MsG0780040726.01	0.853519	2.264385e-61	4.906144e-58
MsG0180000267.01	MsG0780041161.01	0.805935	1.049864e-49	5.625789e-47
MsG0180000267.01	MsG0780041376.01	0.804282	2.328432e-49	1.195713e-46
MsG0180000267.01	MsG0780041399.01	0.807527	4.839010e-50	2.701509e-47
MsG0180000267.01	MsG0880042078.01	0.809994	1.436765e-50	8.560944e-48
MsG0180000267.01	MsG0880042611.01	0.865236	6.837569e-65	2.208660e-61
MsG0180000267.01	MsG0880042843.01	0.846624	1.932849e-59	3.346886e-56
MsG0180000267.01	MsG0880043172.01	0.800272	1.557549e-48	7.221957e-46
MsG0180000267.01	MsG0880043287.01	0.809372	1.954911e-50	1.145801e-47
MsG0180000267.01	MsG0880043288.01	0.807793	4.248602e-50	2.388412e-47
MsG0180000267.01	MsG0880043303.01	0.807467	4.983645e-50	2.778035e-47
MsG0180000267.01	MsG0880045106.01	0.803738	3.020119e-49	1.529455e-46
MsG0180000267.01	MsG0880045803.01	0.820558	6.436174e-53	5.109576e-50
MsG0180000267.01	MsG0880046244.01	0.818776	1.643281e-52	1.241610e-49
MsG0180000267.01	MsG0880046440.01	0.829172	5.971841e-55	6.064866e-52
MsG0180000267.01	MsG0880046669.01	0.800800	1.216094e-48	5.714560e-46
MsG0180000267.01	MsG0880046731.01	0.812326	4.485223e-51	2.843308e-48
MsG0180000267.01	MsG0880046733.01	0.819771	9.750240e-53	7.573667e-50
MsG0180000267.01	MsG0880047019.01	0.808946	2.411793e-50	1.397496e-47
MsG0180000267.01	MsG0880047425.01	0.836277	1.028337e-56	1.289845e-53
MsG0180000267.01	MsG0880047497.01	0.817139	3.850912e-52	2.781577e-49
MsG0180000267.01	MsG0880047608.01	0.800572	1.353279e-48	6.322702e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000267.01.T01	MTR_1g019490	98.413	63	1	0	1	63	789	851	3.61e-36	127
MsG0180000267.01.T01	MTR_3g101660	76.923	65	15	0	1	65	807	871	2.67e-27	102

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000267.01.T01	AT2G26570	66.667	54	18	1	54	680	733	8.45e-18	75.5
MsG0180000267.01.T01	AT2G26570	66.667	54	18	1	54	680	733	8.45e-18	75.5
MsG0180000267.01.T01	AT4G33390	62.264	53	20	1	53	672	724	9.40e-14	63.9
MsG0180000267.01.T01	AT5G42880	62.000	50	19	1	50	618	667	5.56e-13	62.0

Find 17 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTCTGGTTTACTACATTT+TGG	0.153241	1:-3529355	None:intergenic
CGGAGAAAAGCCGGTGAAAT+AGG	0.268026	1:+3529332	MsG0180000267.01.T01:CDS
TTTACCACTATGATTTGTTC+TGG	0.329950	1:-3529370	None:intergenic
AAGGCAAGGGAAGGAAAATT+AGG	0.367717	1:+3529266	MsG0180000267.01.T01:CDS
ACAAAGAAATAGTTGCTAGA+AGG	0.385216	1:+3529210	MsG0180000267.01.T01:CDS
CGCTTCATTCAACTTCTCCA+TGG	0.443325	1:-3529186	None:intergenic
TAGAGCAAGAATTAAGAAAG+TGG	0.463769	1:+3529291	MsG0180000267.01.T01:CDS
CAAAGAAATAGTTGCTAGAA+GGG	0.488221	1:+3529211	MsG0180000267.01.T01:CDS
TGGATAAATCTGAGAAGGCA+AGG	0.529566	1:+3529252	MsG0180000267.01.T01:CDS
CATGGACAACGGAGAAAAGC+CGG	0.537596	1:+3529323	MsG0180000267.01.T01:CDS
GGAATCATTGAAGATTGCTA+TGG	0.544639	1:+3529232	MsG0180000267.01.T01:CDS
TGCTATGGATAAATCTGAGA+AGG	0.581662	1:+3529247	MsG0180000267.01.T01:CDS
GGATAAATCTGAGAAGGCAA+GGG	0.599722	1:+3529253	MsG0180000267.01.T01:CDS
AGAAAGTGGAGAGCAGAACA+TGG	0.609255	1:+3529305	MsG0180000267.01.T01:CDS
AAATCTGAGAAGGCAAGGGA+AGG	0.626732	1:+3529257	MsG0180000267.01.T01:CDS
TAAACCAGAACAAATCATAG+TGG	0.627848	1:+3529366	MsG0180000267.01.T01:CDS
GGAGAGCAGAACATGGACAA+CGG	0.701482	1:+3529312	MsG0180000267.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAAAGAAATAGTTGCTAGA+AGG	+	Chr1:3529210-3529229	MsG0180000267.01.T01:CDS	30.0%
	CAAAGAAATAGTTGCTAGAA+GGG	+	Chr1:3529211-3529230	MsG0180000267.01.T01:CDS	30.0%
	TAGAGCAAGAATTAAGAAAG+TGG	+	Chr1:3529291-3529310	MsG0180000267.01.T01:CDS	30.0%
!!!	TGTTCTGGTTTACTACATTT+TGG	-	Chr1:3529358-3529377	None:intergenic	30.0%
	GGAATCATTGAAGATTGCTA+TGG	+	Chr1:3529232-3529251	MsG0180000267.01.T01:CDS	35.0%
	TGCTATGGATAAATCTGAGA+AGG	+	Chr1:3529247-3529266	MsG0180000267.01.T01:CDS	35.0%
!!	TACATTTTGGCCTATTTCAC+CGG	-	Chr1:3529345-3529364	None:intergenic	35.0%
	AAGGCAAGGGAAGGAAAATT+AGG	+	Chr1:3529266-3529285	MsG0180000267.01.T01:CDS	40.0%
	GGATAAATCTGAGAAGGCAA+GGG	+	Chr1:3529253-3529272	MsG0180000267.01.T01:CDS	40.0%
	TGGATAAATCTGAGAAGGCA+AGG	+	Chr1:3529252-3529271	MsG0180000267.01.T01:CDS	40.0%
	AAATCTGAGAAGGCAAGGGA+AGG	+	Chr1:3529257-3529276	MsG0180000267.01.T01:CDS	45.0%
	AGAAAGTGGAGAGCAGAACA+TGG	+	Chr1:3529305-3529324	MsG0180000267.01.T01:CDS	45.0%
	CATGGACAACGGAGAAAAGC+CGG	+	Chr1:3529323-3529342	MsG0180000267.01.T01:CDS	50.0%
	CGGAGAAAAGCCGGTGAAAT+AGG	+	Chr1:3529332-3529351	MsG0180000267.01.T01:CDS	50.0%
	GGAGAGCAGAACATGGACAA+CGG	+	Chr1:3529312-3529331	MsG0180000267.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	3529188	3529385	3529188	ID=MsG0180000267.01;Name=MsG0180000267.01
Chr1	mRNA	3529188	3529385	3529188	ID=MsG0180000267.01.T01;Parent=MsG0180000267.01;Name=MsG0180000267.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|65
Chr1	exon	3529188	3529385	3529188	ID=MsG0180000267.01.T01:exon:5988;Parent=MsG0180000267.01.T01
Chr1	CDS	3529188	3529385	3529188	ID=MsG0180000267.01.T01:cds;Parent=MsG0180000267.01.T01

Gene Sequence	>MsG0180000267.01.T01 ATGGAGAAGTTGAATGAAGCGAACAAAGAAATAGTTGCTAGAAGGGAATCATTGAAGATTGCTATGGATAAATCTGAGAAGGCAAGGGAAGGAAAATTAGGTGTAGAGCAAGAATTAAGAAAGTGGAGAGCAGAACATGGACAACGGAGAAAAGCCGGTGAAATAGGCCAAAATGTAGTAAACCAGAACAAATCATAG
Protein sequence	>MsG0180000267.01.T01 MEKLNEANKEIVARRESLKIAMDKSEKAREGKLGVEQELRKWRAEHGQRRKAGEIGQNVVNQNKS*