Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000266.01.T01 | XP_039688711.1 | 92.661 | 109 | 8 | 0 | 2 | 110 | 332 | 440 | 3.55E-53 | 189 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000266.01.T01 | O48724 | 73.786 | 103 | 27 | 0 | 2 | 104 | 220 | 322 | 5.93E-39 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000266.01.T01 | G7I3K8 | 92.661 | 109 | 8 | 0 | 2 | 110 | 329 | 437 | 1.73e-53 | 189 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048035.01 | MsG0180000266.01 | 0.802724 | 4.898238e-49 | 2.417060e-46 |
| MsG0080048915.01 | MsG0180000266.01 | 0.821304 | 4.336080e-53 | 3.514466e-50 |
| MsG0180000266.01 | MsG0180000267.01 | 0.825434 | 4.697166e-54 | 4.280633e-51 |
| MsG0180000266.01 | MsG0180002912.01 | 0.864438 | 1.215519e-64 | 3.816882e-61 |
| MsG0180000266.01 | MsG0180004462.01 | 0.820090 | 8.239581e-53 | 6.457842e-50 |
| MsG0180000266.01 | MsG0180004464.01 | 0.804249 | 2.365363e-49 | 1.213605e-46 |
| MsG0180000266.01 | MsG0180005172.01 | 0.838145 | 3.420842e-57 | 4.540619e-54 |
| MsG0180000266.01 | MsG0280006356.01 | 0.821374 | 4.176277e-53 | 3.391546e-50 |
| MsG0180000266.01 | MsG0280007272.01 | 0.811536 | 6.665094e-51 | 4.136553e-48 |
| MsG0180000266.01 | MsG0280008220.01 | 0.805299 | 1.427816e-49 | 7.526844e-47 |
| MsG0180000266.01 | MsG0280009642.01 | 0.808817 | 2.569973e-50 | 1.484073e-47 |
| MsG0180000266.01 | MsG0280010459.01 | 0.805005 | 1.644923e-49 | 8.606154e-47 |
| MsG0180000266.01 | MsG0280010614.01 | 0.822273 | 2.587542e-53 | 2.155637e-50 |
| MsG0180000266.01 | MsG0280010739.01 | 0.804255 | 2.358201e-49 | 1.210134e-46 |
| MsG0180000266.01 | MsG0280011024.01 | 0.820224 | 7.679611e-53 | 6.040529e-50 |
| MsG0180000266.01 | MsG0280011446.01 | 0.823743 | 1.175435e-53 | 1.020969e-50 |
| MsG0180000266.01 | MsG0380011754.01 | 0.805241 | 1.467670e-49 | 7.725509e-47 |
| MsG0180000266.01 | MsG0380012033.01 | 0.800410 | 1.460598e-48 | 6.796206e-46 |
| MsG0180000266.01 | MsG0380013047.01 | 0.824009 | 1.017963e-53 | 8.906837e-51 |
| MsG0180000266.01 | MsG0380014135.01 | 0.803036 | 4.221979e-49 | 2.099828e-46 |
| MsG0180000266.01 | MsG0380014573.01 | 0.820912 | 5.338415e-53 | 4.280118e-50 |
| MsG0180000266.01 | MsG0380015931.01 | 0.834831 | 2.386703e-56 | 2.865950e-53 |
| MsG0180000266.01 | MsG0380015986.01 | 0.802953 | 4.392318e-49 | 2.179956e-46 |
| MsG0180000266.01 | MsG0380017286.01 | 0.813001 | 3.191471e-51 | 2.059613e-48 |
| MsG0180000266.01 | MsG0380017403.01 | 0.803091 | 4.113524e-49 | 2.048671e-46 |
| MsG0180000266.01 | MsG0380017940.01 | 0.805515 | 1.285917e-49 | 6.816465e-47 |
| MsG0180000266.01 | MsG0380018009.01 | 0.817467 | 3.249067e-52 | 2.368141e-49 |
| MsG0180000266.01 | MsG0480018970.01 | 0.808410 | 3.139926e-50 | 1.794026e-47 |
| MsG0180000266.01 | MsG0480020444.01 | 0.818613 | 1.788564e-52 | 1.345223e-49 |
| MsG0180000266.01 | MsG0480020445.01 | 0.842605 | 2.335087e-58 | 3.559111e-55 |
| MsG0180000266.01 | MsG0480020886.01 | 0.812427 | 4.262959e-51 | 2.709820e-48 |
| MsG0180000266.01 | MsG0580024407.01 | 0.806836 | 6.779411e-50 | 3.717500e-47 |
| MsG0180000266.01 | MsG0580027129.01 | 0.844909 | 5.643906e-59 | 9.247676e-56 |
| MsG0180000266.01 | MsG0580027345.01 | 0.808836 | 2.546999e-50 | 1.471471e-47 |
| MsG0180000266.01 | MsG0580027847.01 | 0.835898 | 1.283476e-56 | 1.591281e-53 |
| MsG0180000266.01 | MsG0580029906.01 | 0.807469 | 4.978101e-50 | 2.775094e-47 |
| MsG0180000266.01 | MsG0680030316.01 | 0.805415 | 1.349925e-49 | 7.137515e-47 |
| MsG0180000266.01 | MsG0680034450.01 | 0.840858 | 6.751435e-58 | 9.743540e-55 |
| MsG0180000266.01 | MsG0780037109.01 | 0.845781 | 3.277775e-59 | 5.524044e-56 |
| MsG0180000266.01 | MsG0780037515.01 | 0.809767 | 1.607871e-50 | 9.523689e-48 |
| MsG0180000266.01 | MsG0780038444.01 | 0.805750 | 1.147930e-49 | 6.122574e-47 |
| MsG0180000266.01 | MsG0780040442.01 | 0.843234 | 1.588529e-58 | 2.469333e-55 |
| MsG0180000266.01 | MsG0880042589.01 | 0.809905 | 1.502004e-50 | 8.928344e-48 |
| MsG0180000266.01 | MsG0880042843.01 | 0.839715 | 1.342419e-57 | 1.869588e-54 |
| MsG0180000266.01 | MsG0880042967.01 | 0.816681 | 4.878736e-52 | 3.479925e-49 |
| MsG0180000266.01 | MsG0880045290.01 | 0.804199 | 2.422847e-49 | 1.241543e-46 |
| MsG0180000266.01 | MsG0880046753.01 | 0.810287 | 1.242417e-50 | 7.459150e-48 |
| MsG0180000266.01 | MsG0880047497.01 | 0.801623 | 8.252940e-49 | 3.959539e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000266.01.T01 | MTR_1g019490 | 92.661 | 109 | 8 | 0 | 2 | 110 | 329 | 437 | 4.38e-57 | 189 |
| MsG0180000266.01.T01 | MTR_3g101660 | 79.630 | 108 | 19 | 1 | 2 | 106 | 346 | 453 | 2.47e-45 | 156 |
| MsG0180000266.01.T01 | MTR_8g070840 | 45.745 | 94 | 51 | 0 | 8 | 101 | 68 | 161 | 2.96e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000266.01.T01 | AT2G26570 | 73.786 | 103 | 27 | 0 | 2 | 104 | 220 | 322 | 6.05e-40 | 141 |
| MsG0180000266.01.T01 | AT2G26570 | 73.786 | 103 | 27 | 0 | 2 | 104 | 220 | 322 | 6.05e-40 | 141 |
| MsG0180000266.01.T01 | AT4G33390 | 69.000 | 100 | 31 | 0 | 5 | 104 | 214 | 313 | 2.22e-35 | 128 |
| MsG0180000266.01.T01 | AT1G45545 | 59.406 | 101 | 41 | 0 | 4 | 104 | 212 | 312 | 7.87e-22 | 89.7 |
| MsG0180000266.01.T01 | AT1G45545 | 59.406 | 101 | 41 | 0 | 4 | 104 | 213 | 313 | 8.03e-22 | 89.7 |
| MsG0180000266.01.T01 | AT5G42880 | 57.426 | 101 | 43 | 0 | 4 | 104 | 191 | 291 | 8.63e-20 | 84.0 |
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTCTTTAGTTGATGAAA+AGG | 0.390393 | 1:+3528103 | MsG0180000266.01.T01:CDS |
| TTGCCTTGCTTGATGCTCTT+CGG | 0.398129 | 1:-3527913 | None:intergenic |
| GGCACTCGTATACCTTGCTT+TGG | 0.399846 | 1:-3528027 | None:intergenic |
| CGATGCAGCAACAGCATCTT+CGG | 0.403753 | 1:-3528143 | None:intergenic |
| CTTCGGTTTGTGCCCTCTCT+AGG | 0.404658 | 1:-3527896 | None:intergenic |
| GGCTGCCACACTGGAATCTT+CGG | 0.417044 | 1:-3527985 | None:intergenic |
| GGCAACCTCGAGTTGCGCCT+TGG | 0.433398 | 1:-3528006 | None:intergenic |
| TTGCGCCTTGGCTGCCACAC+TGG | 0.466144 | 1:-3527994 | None:intergenic |
| AAAGCTCAGAGTGGAAGAAA+TGG | 0.509731 | 1:+3527950 | MsG0180000266.01.T01:CDS |
| AGATTCCAGTGTGGCAGCCA+AGG | 0.524007 | 1:+3527989 | MsG0180000266.01.T01:CDS |
| CACCGATGTTAACTCTGTAA+TGG | 0.538415 | 1:-3528048 | None:intergenic |
| TGTCAAGCTCCTGAAGTACT+TGG | 0.546825 | 1:-3527839 | None:intergenic |
| AACTCGAGGTTGCCAAAGCA+AGG | 0.569107 | 1:+3528015 | MsG0180000266.01.T01:CDS |
| AACTAAAACTGAGCCTAGAG+AGG | 0.574371 | 1:+3527883 | MsG0180000266.01.T01:CDS |
| CGAGTTAGATTCGTTGCGTG+TGG | 0.575491 | 1:+3528076 | MsG0180000266.01.T01:CDS |
| GGCAGCCAAGGCGCAACTCG+AGG | 0.584945 | 1:+3528001 | MsG0180000266.01.T01:CDS |
| AAACCGAAGAGCATCAAGCA+AGG | 0.595409 | 1:+3527910 | MsG0180000266.01.T01:CDS |
| CACACGCAACGAATCTAACT+CGG | 0.626532 | 1:-3528075 | None:intergenic |
| AGAACTTGCAAAGCTCAGAG+TGG | 0.657807 | 1:+3527941 | MsG0180000266.01.T01:CDS |
| ACTAAAACTGAGCCTAGAGA+GGG | 0.671828 | 1:+3527884 | MsG0180000266.01.T01:CDS |
| AGAGTGGAAGAAATGGAGCA+AGG | 0.675194 | 1:+3527957 | MsG0180000266.01.T01:CDS |
| TGCCATTACAGAGTTAACAT+CGG | 0.702576 | 1:+3528046 | MsG0180000266.01.T01:CDS |
| TATTGCCGAAGATTCCAGTG+TGG | 0.782850 | 1:+3527980 | MsG0180000266.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTTGGACTTTTTCTTGTT+CGG | - | Chr1:3527826-3527845 | None:intergenic | 30.0% |
| AGAAAAAGTCCAAGTACTTC+AGG | + | Chr1:3527830-3527849 | MsG0180000266.01.T01:CDS | 35.0% | |
| TGCCATTACAGAGTTAACAT+CGG | + | Chr1:3528046-3528065 | MsG0180000266.01.T01:CDS | 35.0% | |
| !! | CGCTTCTTTAGTTGATGAAA+AGG | + | Chr1:3528103-3528122 | MsG0180000266.01.T01:CDS | 35.0% |
| AAAGCTCAGAGTGGAAGAAA+TGG | + | Chr1:3527950-3527969 | MsG0180000266.01.T01:CDS | 40.0% | |
| AACTAAAACTGAGCCTAGAG+AGG | + | Chr1:3527883-3527902 | MsG0180000266.01.T01:CDS | 40.0% | |
| ACTAAAACTGAGCCTAGAGA+GGG | + | Chr1:3527884-3527903 | MsG0180000266.01.T01:CDS | 40.0% | |
| CACCGATGTTAACTCTGTAA+TGG | - | Chr1:3528051-3528070 | None:intergenic | 40.0% | |
| AGAACTTGCAAAGCTCAGAG+TGG | + | Chr1:3527941-3527960 | MsG0180000266.01.T01:CDS | 45.0% | |
| AGAGTGGAAGAAATGGAGCA+AGG | + | Chr1:3527957-3527976 | MsG0180000266.01.T01:CDS | 45.0% | |
| CACACGCAACGAATCTAACT+CGG | - | Chr1:3528078-3528097 | None:intergenic | 45.0% | |
| TATTGCCGAAGATTCCAGTG+TGG | + | Chr1:3527980-3527999 | MsG0180000266.01.T01:CDS | 45.0% | |
| TTGCCTTGCTTGATGCTCTT+CGG | - | Chr1:3527916-3527935 | None:intergenic | 45.0% | |
| ! | TGTCAAGCTCCTGAAGTACT+TGG | - | Chr1:3527842-3527861 | None:intergenic | 45.0% |
| !! | AAACCGAAGAGCATCAAGCA+AGG | + | Chr1:3527910-3527929 | MsG0180000266.01.T01:CDS | 45.0% |
| AACTCGAGGTTGCCAAAGCA+AGG | + | Chr1:3528015-3528034 | MsG0180000266.01.T01:CDS | 50.0% | |
| CGAGTTAGATTCGTTGCGTG+TGG | + | Chr1:3528076-3528095 | MsG0180000266.01.T01:CDS | 50.0% | |
| CGATGCAGCAACAGCATCTT+CGG | - | Chr1:3528146-3528165 | None:intergenic | 50.0% | |
| GGCACTCGTATACCTTGCTT+TGG | - | Chr1:3528030-3528049 | None:intergenic | 50.0% | |
| AGATTCCAGTGTGGCAGCCA+AGG | + | Chr1:3527989-3528008 | MsG0180000266.01.T01:CDS | 55.0% | |
| GGCTGCCACACTGGAATCTT+CGG | - | Chr1:3527988-3528007 | None:intergenic | 55.0% | |
| ! | CTTCGGTTTGTGCCCTCTCT+AGG | - | Chr1:3527899-3527918 | None:intergenic | 55.0% |
| TTGCGCCTTGGCTGCCACAC+TGG | - | Chr1:3527997-3528016 | None:intergenic | 65.0% | |
| ! | GGCAACCTCGAGTTGCGCCT+TGG | - | Chr1:3528009-3528028 | None:intergenic | 65.0% |
| GGCAGCCAAGGCGCAACTCG+AGG | + | Chr1:3528001-3528020 | MsG0180000266.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 3527813 | 3528175 | 3527813 | ID=MsG0180000266.01;Name=MsG0180000266.01 |
| Chr1 | mRNA | 3527813 | 3528175 | 3527813 | ID=MsG0180000266.01.T01;Parent=MsG0180000266.01;Name=MsG0180000266.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|120 |
| Chr1 | exon | 3527813 | 3528175 | 3527813 | ID=MsG0180000266.01.T01:exon:5975;Parent=MsG0180000266.01.T01 |
| Chr1 | CDS | 3527813 | 3528175 | 3527813 | ID=MsG0180000266.01.T01:cds;Parent=MsG0180000266.01.T01 |
| Gene Sequence |
| Protein sequence |
