AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048915.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048915.01.T01 MTR_2g096870 95.833 72 3 0 1 72 659 730 5.07e-41 141
MsG0080048915.01.T01 MTR_2g096870 95.833 72 3 0 1 72 621 692 5.43e-41 141
MsG0080048915.01.T01 MTR_1g047660 44.928 69 38 0 4 72 779 847 3.87e-14 65.5
MsG0080048915.01.T01 MTR_4g021865 44.118 68 38 0 4 71 361 428 3.06e-13 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048915.01.T01 AT1G07620 50.685 73 33 2 1 70 503 575 3.08e-13 62.8
MsG0080048915.01.T01 AT1G07620 50.685 73 33 2 1 70 477 549 3.16e-13 62.8
MsG0080048915.01.T01 AT1G07620 50.685 73 33 2 1 70 477 549 3.16e-13 62.8
MsG0080048915.01.T01 AT1G07620 50.685 73 33 2 1 70 477 549 3.16e-13 62.8
MsG0080048915.01.T01 AT5G02390 46.032 63 33 1 1 62 762 824 1.03e-12 61.6
MsG0080048915.01.T01 AT5G02390 46.032 63 33 1 1 62 762 824 1.03e-12 61.6

Find 13 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATCTTGTTAAAGAAGTA+TGG 0.258917 contig535end:+9137 MsG0080048915.01.T01:CDS
TTAACAAGATAGTTCCCTTT+AGG 0.319585 contig535end:-9127 None:intergenic
AACTCAAACCAATGCCTAAA+GGG 0.384062 contig535end:+9113 None:intergenic
AGATAGTTCCCTTTAGGCAT+TGG 0.454693 contig535end:-9121 MsG0080048915.01.T01:intergenic
CAGACATTAGATGATGTTGT+TGG 0.474147 contig535end:+9202 MsG0080048915.01.T01:CDS
AAACTCAAACCAATGCCTAA+AGG 0.488700 contig535end:+9112 None:intergenic
TGGTTGGATGAATCTTCAAC+AGG 0.489385 contig535end:+9246 MsG0080048915.01.T01:CDS
GATGAATCTTCAACAGGAAG+AGG 0.528776 contig535end:+9252 MsG0080048915.01.T01:CDS
CTATCTTGTTAAAGAAGTAT+GGG 0.540514 contig535end:+9138 MsG0080048915.01.T01:CDS
TCTAATGTCTGATCTAACTC+TGG 0.557841 contig535end:-9190 MsG0080048915.01.T01:intergenic
GTGTGTAGCACTTGAACTTG+AGG 0.590237 contig535end:+9276 MsG0080048915.01.T01:CDS
ATTTGACGAAAAGAAGTGGT+TGG 0.594040 contig535end:+9230 MsG0080048915.01.T01:CDS
CGTGATTTGACGAAAAGAAG+TGG 0.655367 contig535end:+9226 MsG0080048915.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACTATCTTGTTAAAGAAGTA+TGG + contig535end:9137-9156 MsG0080048915.01.T01:CDS 25.0%
!! CTATCTTGTTAAAGAAGTAT+GGG + contig535end:9138-9157 MsG0080048915.01.T01:CDS 25.0%
TTAACAAGATAGTTCCCTTT+AGG - contig535end:9130-9149 MsG0080048915.01.T01:intergenic 30.0%
ATTTGACGAAAAGAAGTGGT+TGG + contig535end:9230-9249 MsG0080048915.01.T01:CDS 35.0%
CAGACATTAGATGATGTTGT+TGG + contig535end:9202-9221 MsG0080048915.01.T01:CDS 35.0%
TCTAATGTCTGATCTAACTC+TGG - contig535end:9193-9212 MsG0080048915.01.T01:intergenic 35.0%
CGTGATTTGACGAAAAGAAG+TGG + contig535end:9226-9245 MsG0080048915.01.T01:CDS 40.0%
GATGAATCTTCAACAGGAAG+AGG + contig535end:9252-9271 MsG0080048915.01.T01:CDS 40.0%
TGGTTGGATGAATCTTCAAC+AGG + contig535end:9246-9265 MsG0080048915.01.T01:CDS 40.0%
GTGTGTAGCACTTGAACTTG+AGG + contig535end:9276-9295 MsG0080048915.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
contig535end gene 9124 9342 9124 ID=MsG0080048915.01;Name=MsG0080048915.01
contig535end mRNA 9124 9342 9124 ID=MsG0080048915.01.T01;Parent=MsG0080048915.01;Name=MsG0080048915.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72
contig535end exon 9124 9342 9124 ID=MsG0080048915.01.T01:exon:37578;Parent=MsG0080048915.01.T01
contig535end CDS 9124 9342 9124 ID=MsG0080048915.01.T01:cds;Parent=MsG0080048915.01.T01
Gene Sequence

>MsG0080048915.01.T01

ATGCCTAAAGGGAACTATCTTGTTAAAGAAGTATGGGACAGTGTTAGTTCATATTTGATGTTGAGACCAGAGTTAGATCAGACATTAGATGATGTTGTTGGTCGTGATTTGACGAAAAGAAGTGGTTGGATGAATCTTCAACAGGAAGAGGAGTGTGTAGCACTTGAACTTGAGGAAATGATCATAGATGATTTATTAGATGAAATTCTCTTCTCATGA

Protein sequence

>MsG0080048915.01.T01

MPKGNYLVKEVWDSVSSYLMLRPELDQTLDDVVGRDLTKRSGWMNLQQEEECVALELEEMIIDDLLDEILFS*