Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011048.01.T01 | XP_024632562.1 | 97.093 | 172 | 5 | 0 | 1 | 172 | 1 | 172 | 7.65E-120 | 345 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011048.01.T01 | A2YEZ6 | 69.54 | 174 | 48 | 3 | 1 | 172 | 1 | 171 | 2.63E-84 | 248 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011048.01.T01 | B7FGP6 | 97.093 | 172 | 5 | 0 | 1 | 172 | 1 | 172 | 3.66e-120 | 345 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000355.01 | MsG0280011048.01 | -0.849781 | 2.593875e-60 | 4.972416e-57 |
| MsG0180003767.01 | MsG0280011048.01 | 0.819948 | 8.883597e-53 | 6.934223e-50 |
| MsG0180004605.01 | MsG0280011048.01 | 0.803722 | 3.044272e-49 | 1.540988e-46 |
| MsG0280011048.01 | MsG0380016347.01 | 0.844350 | 7.981852e-59 | 1.285045e-55 |
| MsG0280011048.01 | MsG0380016671.01 | -0.813124 | 3.000120e-51 | 1.942366e-48 |
| MsG0280011048.01 | MsG0380016820.01 | -0.805853 | 1.092234e-49 | 5.840776e-47 |
| MsG0280011048.01 | MsG0480021077.01 | 0.837139 | 6.198506e-57 | 7.977968e-54 |
| MsG0280011048.01 | MsG0480022781.01 | 0.856085 | 4.082113e-62 | 9.629325e-59 |
| MsG0280011048.01 | MsG0580025047.01 | 0.814141 | 1.791962e-51 | 1.192753e-48 |
| MsG0280011048.01 | MsG0580025369.01 | 0.823111 | 1.651014e-53 | 1.408603e-50 |
| MsG0280011048.01 | MsG0780039747.01 | 0.800933 | 1.142209e-48 | 5.385871e-46 |
| MsG0280011048.01 | MsG0780039997.01 | 0.824347 | 8.479530e-54 | 7.490787e-51 |
| MsG0280011048.01 | MsG0880043027.01 | 0.807858 | 4.117142e-50 | 2.318271e-47 |
| MsG0280011048.01 | MsG0880044142.01 | -0.815418 | 9.341411e-52 | 6.436717e-49 |
| MsG0280011048.01 | MsG0880045010.01 | 0.865317 | 6.446050e-65 | 2.087946e-61 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0280011048.01 | MsG0780041105.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011048.01.T01 | MTR_2g098160 | 97.093 | 172 | 5 | 0 | 1 | 172 | 1 | 172 | 9.27e-124 | 345 |
| MsG0280011048.01.T01 | MTR_0249s0070 | 61.765 | 170 | 61 | 2 | 3 | 172 | 4 | 169 | 2.76e-76 | 225 |
| MsG0280011048.01.T01 | MTR_0249s0070 | 61.765 | 170 | 61 | 2 | 3 | 172 | 12 | 177 | 3.64e-76 | 225 |
| MsG0280011048.01.T01 | MTR_7g092400 | 63.743 | 171 | 59 | 3 | 3 | 172 | 92 | 260 | 1.94e-75 | 226 |
| MsG0280011048.01.T01 | MTR_4g065570 | 76.923 | 143 | 26 | 2 | 31 | 172 | 1 | 137 | 4.32e-75 | 221 |
| MsG0280011048.01.T01 | MTR_7g104320 | 46.061 | 165 | 79 | 3 | 12 | 172 | 10 | 168 | 1.65e-45 | 147 |
| MsG0280011048.01.T01 | MTR_4g053440 | 45.238 | 168 | 85 | 4 | 8 | 172 | 7 | 170 | 9.55e-43 | 140 |
| MsG0280011048.01.T01 | MTR_2g086190 | 44.937 | 158 | 70 | 5 | 15 | 172 | 10 | 150 | 2.86e-41 | 136 |
| MsG0280011048.01.T01 | MTR_1g060380 | 44.785 | 163 | 74 | 4 | 11 | 172 | 9 | 156 | 2.19e-37 | 126 |
| MsG0280011048.01.T01 | MTR_3g028010 | 37.821 | 156 | 77 | 2 | 17 | 172 | 28 | 163 | 7.51e-29 | 105 |
| MsG0280011048.01.T01 | MTR_8g036980 | 52.222 | 90 | 29 | 1 | 83 | 172 | 76 | 151 | 1.27e-27 | 101 |
| MsG0280011048.01.T01 | MTR_1g100773 | 33.333 | 156 | 84 | 4 | 15 | 170 | 3 | 138 | 7.37e-26 | 96.7 |
| MsG0280011048.01.T01 | MTR_7g114920 | 38.065 | 155 | 69 | 6 | 15 | 169 | 3 | 130 | 6.41e-25 | 94.0 |
| MsG0280011048.01.T01 | MTR_2g054650 | 33.333 | 156 | 70 | 5 | 15 | 170 | 4 | 125 | 6.64e-20 | 80.9 |
| MsG0280011048.01.T01 | MTR_3g025570 | 70.455 | 44 | 13 | 0 | 107 | 150 | 4 | 47 | 3.90e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011048.01.T01 | AT1G51200 | 59.887 | 177 | 62 | 4 | 1 | 172 | 1 | 173 | 3.36e-62 | 190 |
| MsG0280011048.01.T01 | AT1G51200 | 59.887 | 177 | 62 | 4 | 1 | 172 | 1 | 173 | 3.36e-62 | 190 |
| MsG0280011048.01.T01 | AT1G51200 | 59.887 | 177 | 62 | 4 | 1 | 172 | 1 | 173 | 3.36e-62 | 190 |
| MsG0280011048.01.T01 | AT1G51200 | 59.887 | 177 | 62 | 4 | 1 | 172 | 1 | 173 | 3.36e-62 | 190 |
| MsG0280011048.01.T01 | AT2G36320 | 48.485 | 165 | 70 | 4 | 11 | 172 | 9 | 161 | 2.84e-46 | 149 |
| MsG0280011048.01.T01 | AT4G12040 | 43.293 | 164 | 89 | 2 | 12 | 172 | 13 | 175 | 9.72e-46 | 148 |
| MsG0280011048.01.T01 | AT4G12040 | 43.293 | 164 | 89 | 2 | 12 | 172 | 13 | 175 | 9.72e-46 | 148 |
| MsG0280011048.01.T01 | AT2G27580 | 40.741 | 162 | 84 | 2 | 15 | 172 | 10 | 163 | 1.90e-42 | 139 |
| MsG0280011048.01.T01 | AT2G27580 | 40.741 | 162 | 84 | 2 | 15 | 172 | 10 | 163 | 1.90e-42 | 139 |
| MsG0280011048.01.T01 | AT1G12440 | 40.123 | 162 | 92 | 2 | 11 | 172 | 12 | 168 | 5.23e-40 | 133 |
| MsG0280011048.01.T01 | AT1G12440 | 40.123 | 162 | 92 | 2 | 11 | 172 | 12 | 168 | 5.23e-40 | 133 |
| MsG0280011048.01.T01 | AT1G12440 | 40.123 | 162 | 92 | 2 | 11 | 172 | 12 | 168 | 5.23e-40 | 133 |
| MsG0280011048.01.T01 | AT3G52800 | 43.827 | 162 | 91 | 0 | 11 | 172 | 9 | 170 | 4.23e-38 | 129 |
| MsG0280011048.01.T01 | AT3G52800 | 43.827 | 162 | 91 | 0 | 11 | 172 | 9 | 170 | 4.23e-38 | 129 |
| MsG0280011048.01.T01 | AT4G22820 | 38.509 | 161 | 93 | 3 | 15 | 172 | 19 | 176 | 5.01e-37 | 126 |
| MsG0280011048.01.T01 | AT4G22820 | 38.509 | 161 | 93 | 3 | 15 | 172 | 19 | 176 | 5.01e-37 | 126 |
| MsG0280011048.01.T01 | AT3G12630 | 39.333 | 150 | 71 | 2 | 23 | 172 | 31 | 160 | 7.32e-32 | 112 |
| MsG0280011048.01.T01 | AT4G14225 | 37.342 | 158 | 62 | 5 | 15 | 172 | 5 | 125 | 3.37e-27 | 99.8 |
| MsG0280011048.01.T01 | AT4G25380 | 32.903 | 155 | 70 | 4 | 18 | 172 | 10 | 130 | 2.48e-24 | 92.4 |
Find 48 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTTCTGCAGAGATGGTTT+TGG | 0.250126 | 2:+78862662 | None:intergenic |
| GTTTCAGCATCAAGTCTTTG+TGG | 0.370986 | 2:+78862805 | None:intergenic |
| CATCAAGTCTTTGTGGCACT+TGG | 0.400676 | 2:+78862812 | None:intergenic |
| TTCCGGCTCAGAGGAGAGTT+TGG | 0.400683 | 2:-78862631 | MsG0280011048.01.T01:CDS |
| CAAAGACTTGATGCTGAAAC+AGG | 0.401281 | 2:-78862802 | MsG0280011048.01.T01:CDS |
| CCAGCAGTGCGGTAATCAAA+TGG | 0.411400 | 2:+78862480 | None:intergenic |
| GCAGAACCGTTAGTTGCTTC+CGG | 0.413723 | 2:-78862648 | MsG0280011048.01.T01:CDS |
| AATAAGCGGGTTGGTTTGAC+AGG | 0.421004 | 2:-78862567 | MsG0280011048.01.T01:CDS |
| CACTTGCAGCATCTTCCATT+GGG | 0.430249 | 2:-78862770 | MsG0280011048.01.T01:CDS |
| TTGCAGTTGCTGCACCGCTT+AGG | 0.445309 | 2:+78862588 | None:intergenic |
| TCCATTGGGAACATCATGAA+TGG | 0.454077 | 2:-78862756 | MsG0280011048.01.T01:CDS |
| CCATTTGATTACCGCACTGC+TGG | 0.477078 | 2:-78862480 | MsG0280011048.01.T01:CDS |
| ATGCACAACATAGGACCCTC+TGG | 0.486214 | 2:+78862882 | None:intergenic |
| ACACTTGCAGCATCTTCCAT+TGG | 0.488087 | 2:-78862771 | MsG0280011048.01.T01:CDS |
| CTTCACATCCAGTCTGATCA+TGG | 0.503432 | 2:+78862910 | None:intergenic |
| CAGCAGTGCGGTAATCAAAT+GGG | 0.506125 | 2:+78862481 | None:intergenic |
| CACCGCTTAGGACCATCCTT+CGG | 0.507315 | 2:+78862600 | None:intergenic |
| TCCACGCTGGCAGTGATAGC+AGG | 0.514469 | 2:+78862708 | None:intergenic |
| CTCCAAACTCTCCTCTGAGC+CGG | 0.515481 | 2:+78862629 | None:intergenic |
| AACTAACGGTTCTGCAGAGA+TGG | 0.516940 | 2:+78862656 | None:intergenic |
| AGGTTCAATTACACTGCTGC+TGG | 0.521288 | 2:+78862728 | None:intergenic |
| GTGCAGCAACTGCAATAAGC+GGG | 0.530850 | 2:-78862580 | MsG0280011048.01.T01:CDS |
| CCATTGGGAACATCATGAAT+GGG | 0.543973 | 2:-78862755 | MsG0280011048.01.T01:CDS |
| AAAAGCAAACCCTGTTGTGA+AGG | 0.563268 | 2:-78862442 | MsG0280011048.01.T01:CDS |
| GCACTTGGAACACATGTTCA+TGG | 0.566316 | 2:+78862827 | None:intergenic |
| TTGGAGAAGAAGCCGAAGGA+TGG | 0.571839 | 2:-78862612 | MsG0280011048.01.T01:CDS |
| CTTGATGCTGAAACAGGAGC+AGG | 0.574472 | 2:-78862796 | MsG0280011048.01.T01:CDS |
| CCCATTCATGATGTTCCCAA+TGG | 0.580215 | 2:+78862755 | None:intergenic |
| TGCACAACATAGGACCCTCT+GGG | 0.580560 | 2:+78862883 | None:intergenic |
| AGCGATGTACAGCACAGTAA+AGG | 0.582881 | 2:+78862523 | None:intergenic |
| AGCAACTGCAATAAGCGGGT+TGG | 0.585228 | 2:-78862576 | MsG0280011048.01.T01:CDS |
| ACAGGATTTAATTGTCGATG+TGG | 0.589267 | 2:-78862549 | MsG0280011048.01.T01:CDS |
| CAGTTGTTGATGCACAACAT+AGG | 0.601548 | 2:+78862873 | None:intergenic |
| GGAGATCTCAGTCGATCAAG+TGG | 0.604936 | 2:-78862688 | MsG0280011048.01.T01:CDS |
| AAGATGGACCATGATCAGAC+TGG | 0.612119 | 2:-78862918 | MsG0280011048.01.T01:CDS |
| GGATGTGAAGCTGCCCCAGA+GGG | 0.615247 | 2:-78862897 | MsG0280011048.01.T01:CDS |
| TCTGAGCCGGAAGCAACTAA+CGG | 0.631746 | 2:+78862642 | None:intergenic |
| GAGTTTGGAGAAGAAGCCGA+AGG | 0.631849 | 2:-78862616 | MsG0280011048.01.T01:CDS |
| GGTGCAGCAACTGCAATAAG+CGG | 0.632084 | 2:-78862581 | MsG0280011048.01.T01:CDS |
| AATGGAAGATGCTGCAAGTG+TGG | 0.642024 | 2:+78862773 | None:intergenic |
| GTTAGTTGCTTCCGGCTCAG+AGG | 0.650978 | 2:-78862640 | MsG0280011048.01.T01:CDS |
| ATCGACTGAGATCTCCACGC+TGG | 0.665451 | 2:+78862695 | None:intergenic |
| TGGATGTGAAGCTGCCCCAG+AGG | 0.681846 | 2:-78862898 | MsG0280011048.01.T01:CDS |
| ACCTGCTATCACTGCCAGCG+TGG | 0.686751 | 2:-78862709 | MsG0280011048.01.T01:CDS |
| AGCCGAAGGATGGTCCTAAG+CGG | 0.700565 | 2:-78862602 | MsG0280011048.01.T01:CDS |
| ATGTTGTGCATCAACAACTG+TGG | 0.726175 | 2:-78862870 | MsG0280011048.01.T01:CDS |
| GCACAACATAGGACCCTCTG+GGG | 0.736272 | 2:+78862884 | None:intergenic |
| TTGCATCACGTCCAGCAGTG+CGG | 0.742537 | 2:+78862469 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCAACAACTGTGGATTTTT+CGG | - | Chr2:78862473-78862492 | MsG0280011048.01.T01:CDS | 30.0% |
| ACAGGATTTAATTGTCGATG+TGG | - | Chr2:78862785-78862804 | MsG0280011048.01.T01:CDS | 35.0% | |
| AAAAGCAAACCCTGTTGTGA+AGG | - | Chr2:78862892-78862911 | MsG0280011048.01.T01:CDS | 40.0% | |
| ATGTTGTGCATCAACAACTG+TGG | - | Chr2:78862464-78862483 | MsG0280011048.01.T01:CDS | 40.0% | |
| CAAAGACTTGATGCTGAAAC+AGG | - | Chr2:78862532-78862551 | MsG0280011048.01.T01:CDS | 40.0% | |
| CAGTTGTTGATGCACAACAT+AGG | + | Chr2:78862464-78862483 | None:intergenic | 40.0% | |
| CCATTGGGAACATCATGAAT+GGG | - | Chr2:78862579-78862598 | MsG0280011048.01.T01:CDS | 40.0% | |
| GTTTCAGCATCAAGTCTTTG+TGG | + | Chr2:78862532-78862551 | None:intergenic | 40.0% | |
| TCCATTGGGAACATCATGAA+TGG | - | Chr2:78862578-78862597 | MsG0280011048.01.T01:CDS | 40.0% | |
| AACTAACGGTTCTGCAGAGA+TGG | + | Chr2:78862681-78862700 | None:intergenic | 45.0% | |
| AATGGAAGATGCTGCAAGTG+TGG | + | Chr2:78862564-78862583 | None:intergenic | 45.0% | |
| ACACTTGCAGCATCTTCCAT+TGG | - | Chr2:78862563-78862582 | MsG0280011048.01.T01:CDS | 45.0% | |
| AGCGATGTACAGCACAGTAA+AGG | + | Chr2:78862814-78862833 | None:intergenic | 45.0% | |
| AGGTTCAATTACACTGCTGC+TGG | + | Chr2:78862609-78862628 | None:intergenic | 45.0% | |
| CACTTGCAGCATCTTCCATT+GGG | - | Chr2:78862564-78862583 | MsG0280011048.01.T01:CDS | 45.0% | |
| CAGCAGTGCGGTAATCAAAT+GGG | + | Chr2:78862856-78862875 | None:intergenic | 45.0% | |
| CATCAAGTCTTTGTGGCACT+TGG | + | Chr2:78862525-78862544 | None:intergenic | 45.0% | |
| CCCATTCATGATGTTCCCAA+TGG | + | Chr2:78862582-78862601 | None:intergenic | 45.0% | |
| CTTCACATCCAGTCTGATCA+TGG | + | Chr2:78862427-78862446 | None:intergenic | 45.0% | |
| GCACTTGGAACACATGTTCA+TGG | + | Chr2:78862510-78862529 | None:intergenic | 45.0% | |
| ! | AGCTTTTCAGCCTTCACAAC+AGG | + | Chr2:78862905-78862924 | None:intergenic | 45.0% |
| ! | GCTTTTCAGCCTTCACAACA+GGG | + | Chr2:78862904-78862923 | None:intergenic | 45.0% |
| ! | GGTTCTGCAGAGATGGTTTT+GGG | + | Chr2:78862674-78862693 | None:intergenic | 45.0% |
| !! | AATAAGCGGGTTGGTTTGAC+AGG | - | Chr2:78862767-78862786 | MsG0280011048.01.T01:CDS | 45.0% |
| AGCAACTGCAATAAGCGGGT+TGG | - | Chr2:78862758-78862777 | MsG0280011048.01.T01:CDS | 50.0% | |
| ATGCACAACATAGGACCCTC+TGG | + | Chr2:78862455-78862474 | None:intergenic | 50.0% | |
| CCAGCAGTGCGGTAATCAAA+TGG | + | Chr2:78862857-78862876 | None:intergenic | 50.0% | |
| CCATTTGATTACCGCACTGC+TGG | - | Chr2:78862854-78862873 | MsG0280011048.01.T01:CDS | 50.0% | |
| CTTGATGCTGAAACAGGAGC+AGG | - | Chr2:78862538-78862557 | MsG0280011048.01.T01:CDS | 50.0% | |
| GAGTTTGGAGAAGAAGCCGA+AGG | - | Chr2:78862718-78862737 | MsG0280011048.01.T01:CDS | 50.0% | |
| GCAGAACCGTTAGTTGCTTC+CGG | - | Chr2:78862686-78862705 | MsG0280011048.01.T01:CDS | 50.0% | |
| GGAGATCTCAGTCGATCAAG+TGG | - | Chr2:78862646-78862665 | MsG0280011048.01.T01:CDS | 50.0% | |
| GGTGCAGCAACTGCAATAAG+CGG | - | Chr2:78862753-78862772 | MsG0280011048.01.T01:CDS | 50.0% | |
| GTGCAGCAACTGCAATAAGC+GGG | - | Chr2:78862754-78862773 | MsG0280011048.01.T01:CDS | 50.0% | |
| TCTGAGCCGGAAGCAACTAA+CGG | + | Chr2:78862695-78862714 | None:intergenic | 50.0% | |
| TGCACAACATAGGACCCTCT+GGG | + | Chr2:78862454-78862473 | None:intergenic | 50.0% | |
| TTGGAGAAGAAGCCGAAGGA+TGG | - | Chr2:78862722-78862741 | MsG0280011048.01.T01:CDS | 50.0% | |
| ! | CGGTTCTGCAGAGATGGTTT+TGG | + | Chr2:78862675-78862694 | None:intergenic | 50.0% |
| AGCCGAAGGATGGTCCTAAG+CGG | - | Chr2:78862732-78862751 | MsG0280011048.01.T01:CDS | 55.0% | |
| ATCGACTGAGATCTCCACGC+TGG | + | Chr2:78862642-78862661 | None:intergenic | 55.0% | |
| CACCGCTTAGGACCATCCTT+CGG | + | Chr2:78862737-78862756 | None:intergenic | 55.0% | |
| CTCCAAACTCTCCTCTGAGC+CGG | + | Chr2:78862708-78862727 | None:intergenic | 55.0% | |
| GCACAACATAGGACCCTCTG+GGG | + | Chr2:78862453-78862472 | None:intergenic | 55.0% | |
| GTTAGTTGCTTCCGGCTCAG+AGG | - | Chr2:78862694-78862713 | MsG0280011048.01.T01:CDS | 55.0% | |
| TTCCGGCTCAGAGGAGAGTT+TGG | - | Chr2:78862703-78862722 | MsG0280011048.01.T01:CDS | 55.0% | |
| TTGCAGTTGCTGCACCGCTT+AGG | + | Chr2:78862749-78862768 | None:intergenic | 55.0% | |
| TTGCATCACGTCCAGCAGTG+CGG | + | Chr2:78862868-78862887 | None:intergenic | 55.0% | |
| ACCTGCTATCACTGCCAGCG+TGG | - | Chr2:78862625-78862644 | MsG0280011048.01.T01:CDS | 60.0% | |
| GGATGTGAAGCTGCCCCAGA+GGG | - | Chr2:78862437-78862456 | MsG0280011048.01.T01:CDS | 60.0% | |
| TCCACGCTGGCAGTGATAGC+AGG | + | Chr2:78862629-78862648 | None:intergenic | 60.0% | |
| TGGATGTGAAGCTGCCCCAG+AGG | - | Chr2:78862436-78862455 | MsG0280011048.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 78862419 | 78862937 | 78862419 | ID=MsG0280011048.01;Name=MsG0280011048.01 |
| Chr2 | mRNA | 78862419 | 78862937 | 78862419 | ID=MsG0280011048.01.T01;Parent=MsG0280011048.01;Name=MsG0280011048.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|172 |
| Chr2 | exon | 78862419 | 78862937 | 78862419 | ID=MsG0280011048.01.T01:exon:17347;Parent=MsG0280011048.01.T01 |
| Chr2 | CDS | 78862419 | 78862937 | 78862419 | ID=MsG0280011048.01.T01:cds;Parent=MsG0280011048.01.T01 |
| Gene Sequence |
| Protein sequence |
