AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041105.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041105.01.T01 MTR_7g104320 96.471 170 4 1 1 170 1 168 3.88e-119 333
MsG0780041105.01.T01 MTR_1g060380 58.824 170 56 3 1 170 1 156 3.14e-60 184
MsG0780041105.01.T01 MTR_4g053440 52.571 175 73 4 1 170 1 170 7.29e-59 181
MsG0780041105.01.T01 MTR_2g086190 51.176 170 63 5 1 170 1 150 1.12e-53 167
MsG0780041105.01.T01 MTR_2g098160 42.857 175 87 4 3 170 4 172 6.38e-47 151
MsG0780041105.01.T01 MTR_0249s0070 45.087 173 82 4 3 170 5 169 2.35e-46 149
MsG0780041105.01.T01 MTR_0249s0070 45.087 173 82 4 3 170 13 177 2.71e-46 149
MsG0780041105.01.T01 MTR_7g092400 40.909 176 88 3 3 170 93 260 2.75e-44 147
MsG0780041105.01.T01 MTR_4g065570 38.028 142 83 1 29 170 1 137 1.14e-32 114
MsG0780041105.01.T01 MTR_7g114920 39.610 154 66 4 15 168 5 131 2.57e-30 107
MsG0780041105.01.T01 MTR_1g100773 37.908 153 75 5 15 167 5 137 4.16e-29 104
MsG0780041105.01.T01 MTR_3g028010 34.356 163 73 3 15 170 28 163 2.36e-26 98.6
MsG0780041105.01.T01 MTR_8g036980 59.677 62 25 0 109 170 90 151 1.07e-24 94.0
MsG0780041105.01.T01 MTR_2g054650 36.601 153 63 4 15 167 6 124 2.17e-23 89.7
MsG0780041105.01.T01 MTR_3g025570 60.000 40 16 0 109 148 8 47 1.32e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041105.01.T01 AT2G36320 58.824 170 61 3 1 170 1 161 1.27e-64 196
MsG0780041105.01.T01 AT3G52800 54.070 172 75 3 1 170 1 170 2.04e-52 165
MsG0780041105.01.T01 AT3G52800 54.070 172 75 3 1 170 1 170 2.04e-52 165
MsG0780041105.01.T01 AT2G27580 50.877 171 75 4 1 170 1 163 4.33e-50 159
MsG0780041105.01.T01 AT2G27580 50.877 171 75 4 1 170 1 163 4.33e-50 159
MsG0780041105.01.T01 AT4G22820 48.466 163 72 4 14 170 20 176 1.98e-48 155
MsG0780041105.01.T01 AT4G22820 48.466 163 72 4 14 170 20 176 1.98e-48 155
MsG0780041105.01.T01 AT4G12040 48.795 166 77 3 10 170 13 175 4.80e-48 154
MsG0780041105.01.T01 AT4G12040 48.795 166 77 3 10 170 13 175 4.80e-48 154
MsG0780041105.01.T01 AT1G12440 44.000 175 67 3 9 170 12 168 8.74e-44 143
MsG0780041105.01.T01 AT1G12440 44.000 175 67 3 9 170 12 168 8.74e-44 143
MsG0780041105.01.T01 AT1G12440 44.000 175 67 3 9 170 12 168 8.74e-44 143
MsG0780041105.01.T01 AT1G51200 46.707 167 78 3 10 170 12 173 5.01e-40 133
MsG0780041105.01.T01 AT1G51200 46.707 167 78 3 10 170 12 173 5.01e-40 133
MsG0780041105.01.T01 AT1G51200 46.707 167 78 3 10 170 12 173 5.01e-40 133
MsG0780041105.01.T01 AT1G51200 46.707 167 78 3 10 170 12 173 5.01e-40 133
MsG0780041105.01.T01 AT4G25380 35.484 155 66 3 16 170 10 130 8.46e-27 98.6
MsG0780041105.01.T01 AT3G12630 33.333 150 80 3 21 170 31 160 2.96e-24 93.2
MsG0780041105.01.T01 AT4G14225 35.032 157 63 4 15 170 7 125 5.24e-23 89.0

Find 53 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGCAGAGTCTGTGGCCTTC+TGG 0.265748 7:-86641387 None:intergenic
TTCGATTGTTGATTTAGTTT+TGG 0.276099 7:-86641499 None:intergenic
CTTGTTCTTGTTCCTTCAAA+TGG 0.280650 7:-86641476 None:intergenic
TGTGAATTGTCCGATGCTTC+AGG 0.360056 7:-86641645 None:intergenic
AACGCCGGTGGTTGAGGTTG+AGG 0.369798 7:-86641603 None:intergenic
AGAAAGAGGACCGGGTTAAC+CGG 0.386243 7:+86641702 MsG0780041105.01.T01:CDS
GGAGATAGCGAAAGCGAATC+CGG 0.416264 7:+86641818 MsG0780041105.01.T01:CDS
AGAGGTTTGATTTCCGCAAC+AGG 0.416744 7:-86641579 None:intergenic
GCAAACAACTGCGGCTTCTT+CGG 0.427864 7:+86641408 MsG0780041105.01.T01:CDS
TTGTGGGTCCCACAGGTATC+CGG 0.444625 7:+86641752 MsG0780041105.01.T01:CDS
AGCTTATCAGCTTTGATCAC+CGG 0.447035 7:-86641837 None:intergenic
CGGTTCAATCGAACCGGTGC+GGG 0.475968 7:+86641673 MsG0780041105.01.T01:CDS
GGTGGTTGAGGTTGAGGGAG+AGG 0.476792 7:-86641597 None:intergenic
CGTCGCTGCTTCGCCTGTTG+CGG 0.477966 7:+86641566 MsG0780041105.01.T01:CDS
GACGAACACGGCGGTGGAAG+CGG 0.478022 7:-86641547 None:intergenic
ACGGTCAACGCCGGTGGTTG+AGG 0.481127 7:-86641609 None:intergenic
AACTAAATCAACAATCGAAA+CGG 0.485011 7:+86641503 MsG0780041105.01.T01:CDS
CCGCACCGGTTCGATTGAAC+CGG 0.490138 7:-86641672 None:intergenic
TTACAGAGACATCCATTTGA+AGG 0.490147 7:+86641464 MsG0780041105.01.T01:CDS
AGCAGCGACGAACACGGCGG+TGG 0.501938 7:-86641553 None:intergenic
ACGCCGGTGGTTGAGGTTGA+GGG 0.510233 7:-86641602 None:intergenic
ATCGGACAATTCACAAGGAC+CGG 0.522802 7:+86641653 MsG0780041105.01.T01:CDS
GGTGGAAGCGGATGCTGAGG+CGG 0.523382 7:-86641535 None:intergenic
AAACCGCACTCATGTTTCTC+CGG 0.524064 7:-86641771 None:intergenic
TTTCAAAGCAGTGGGGAGAG+AGG 0.529533 7:+86641797 MsG0780041105.01.T01:CDS
TCTTTCTACACGTCCCGCAC+CGG 0.534045 7:-86641686 None:intergenic
GAAAGAGGACCGGGTTAACC+GGG 0.537492 7:+86641703 MsG0780041105.01.T01:CDS
AAGGACCGGTTCAATCGAAC+CGG 0.537575 7:+86641667 MsG0780041105.01.T01:CDS
GTCGATCGTGCCTGAAGCAT+CGG 0.545884 7:+86641635 MsG0780041105.01.T01:CDS
AGGCGAAGCAGCGACGAACA+CGG 0.549677 7:-86641559 None:intergenic
GGAAGCGGATGCTGAGGCGG+AGG 0.552255 7:-86641532 None:intergenic
AGTTGTTTGCGCAGAGTCTG+TGG 0.555837 7:-86641395 None:intergenic
ACACTTGAACCCGGTTAACC+CGG 0.561755 7:-86641712 None:intergenic
GGCGGTGGAAGCGGATGCTG+AGG 0.566120 7:-86641538 None:intergenic
CATGTTTCTCCGGATACCTG+TGG 0.575785 7:-86641761 None:intergenic
GGTGCGGGACGTGTAGAAAG+AGG 0.587813 7:+86641688 MsG0780041105.01.T01:CDS
ACCGGGTTCAAGTGTAGATG+TGG 0.588078 7:+86641720 MsG0780041105.01.T01:CDS
GACGGTACGGTCAACGCCGG+TGG 0.590519 7:-86641615 None:intergenic
TCCACATCTACACTTGAACC+CGG 0.592518 7:-86641721 None:intergenic
GGGACGTGTAGAAAGAGGAC+CGG 0.594127 7:+86641693 MsG0780041105.01.T01:CDS
GGCCTTCTGGAGTTTCACAT+CGG 0.606780 7:-86641374 None:intergenic
GGACGTGTAGAAAGAGGACC+GGG 0.606988 7:+86641694 MsG0780041105.01.T01:CDS
ATGTTTCTCCGGATACCTGT+GGG 0.618435 7:-86641760 None:intergenic
TTCAGGCACGATCGACGGTA+CGG 0.620849 7:-86641628 None:intergenic
TCTCCCTCAACCTCAACCAC+CGG 0.622826 7:+86641599 MsG0780041105.01.T01:CDS
GATGCTTCAGGCACGATCGA+CGG 0.657260 7:-86641633 None:intergenic
ATCGACGGTACGGTCAACGC+CGG 0.667919 7:-86641618 None:intergenic
CCGGTTCAATCGAACCGGTG+CGG 0.668012 7:+86641672 MsG0780041105.01.T01:CDS
CACCGATGTGAAACTCCAGA+AGG 0.686088 7:+86641372 MsG0780041105.01.T01:CDS
GAAGCATCGGACAATTCACA+AGG 0.714221 7:+86641648 MsG0780041105.01.T01:CDS
AGACTCTGCGCAAACAACTG+CGG 0.735745 7:+86641399 MsG0780041105.01.T01:CDS
TATCCGGAGAAACATGAGTG+CGG 0.743977 7:+86641768 MsG0780041105.01.T01:CDS
CGAAGCAGCGACGAACACGG+CGG 0.752846 7:-86641556 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTAAATCAACAATCGAAA+CGG + Chr7:86641503-86641522 MsG0780041105.01.T01:CDS 25.0%
!!! TTCGATTGTTGATTTAGTTT+TGG - Chr7:86641502-86641521 None:intergenic 25.0%
CTTGTTCTTGTTCCTTCAAA+TGG - Chr7:86641479-86641498 None:intergenic 35.0%
TTACAGAGACATCCATTTGA+AGG + Chr7:86641464-86641483 MsG0780041105.01.T01:CDS 35.0%
! AGATGTGGAATAACGTTTTG+TGG + Chr7:86641735-86641754 MsG0780041105.01.T01:CDS 35.0%
! GATGTGGAATAACGTTTTGT+GGG + Chr7:86641736-86641755 MsG0780041105.01.T01:CDS 35.0%
!!! GGTTTTGATTTCAAAGCAGT+GGG + Chr7:86641789-86641808 MsG0780041105.01.T01:CDS 35.0%
!!! GTTTTGATTTCAAAGCAGTG+GGG + Chr7:86641790-86641809 MsG0780041105.01.T01:CDS 35.0%
! AGCTTATCAGCTTTGATCAC+CGG - Chr7:86641840-86641859 None:intergenic 40.0%
!!! CGGTTTTGATTTCAAAGCAG+TGG + Chr7:86641788-86641807 MsG0780041105.01.T01:CDS 40.0%
AAACCGCACTCATGTTTCTC+CGG - Chr7:86641774-86641793 None:intergenic 45.0%
ATCGGACAATTCACAAGGAC+CGG + Chr7:86641653-86641672 MsG0780041105.01.T01:CDS 45.0%
ATGTTTCTCCGGATACCTGT+GGG - Chr7:86641763-86641782 None:intergenic 45.0%
TATCCGGAGAAACATGAGTG+CGG + Chr7:86641768-86641787 MsG0780041105.01.T01:CDS 45.0%
TCCACATCTACACTTGAACC+CGG - Chr7:86641724-86641743 None:intergenic 45.0%
TGTGAATTGTCCGATGCTTC+AGG - Chr7:86641648-86641667 None:intergenic 45.0%
! AGAGGTTTGATTTCCGCAAC+AGG - Chr7:86641582-86641601 None:intergenic 45.0%
!! GAAGCATCGGACAATTCACA+AGG + Chr7:86641648-86641667 MsG0780041105.01.T01:CDS 45.0%
ACACTTGAACCCGGTTAACC+CGG - Chr7:86641715-86641734 None:intergenic 50.0%
ACCGGGTTCAAGTGTAGATG+TGG + Chr7:86641720-86641739 MsG0780041105.01.T01:CDS 50.0%
AGAAAGAGGACCGGGTTAAC+CGG + Chr7:86641702-86641721 MsG0780041105.01.T01:CDS 50.0%
AGACTCTGCGCAAACAACTG+CGG + Chr7:86641399-86641418 MsG0780041105.01.T01:CDS 50.0%
AGTTGTTTGCGCAGAGTCTG+TGG - Chr7:86641398-86641417 None:intergenic 50.0%
CACCGATGTGAAACTCCAGA+AGG + Chr7:86641372-86641391 MsG0780041105.01.T01:CDS 50.0%
CATGTTTCTCCGGATACCTG+TGG - Chr7:86641764-86641783 None:intergenic 50.0%
GCAAACAACTGCGGCTTCTT+CGG + Chr7:86641408-86641427 MsG0780041105.01.T01:CDS 50.0%
GGAGATAGCGAAAGCGAATC+CGG + Chr7:86641818-86641837 MsG0780041105.01.T01:CDS 50.0%
TTTCAAAGCAGTGGGGAGAG+AGG + Chr7:86641797-86641816 MsG0780041105.01.T01:CDS 50.0%
! AAGGACCGGTTCAATCGAAC+CGG + Chr7:86641667-86641686 MsG0780041105.01.T01:CDS 50.0%
! TAACGTTTTGTGGGTCCCAC+AGG + Chr7:86641745-86641764 MsG0780041105.01.T01:CDS 50.0%
!! GGCCTTCTGGAGTTTCACAT+CGG - Chr7:86641377-86641396 None:intergenic 50.0%
GAAAGAGGACCGGGTTAACC+GGG + Chr7:86641703-86641722 MsG0780041105.01.T01:CDS 55.0%
GATGCTTCAGGCACGATCGA+CGG - Chr7:86641636-86641655 None:intergenic 55.0%
GGACGTGTAGAAAGAGGACC+GGG + Chr7:86641694-86641713 MsG0780041105.01.T01:CDS 55.0%
GGGACGTGTAGAAAGAGGAC+CGG + Chr7:86641693-86641712 MsG0780041105.01.T01:CDS 55.0%
GTCGATCGTGCCTGAAGCAT+CGG + Chr7:86641635-86641654 MsG0780041105.01.T01:CDS 55.0%
TCTCCCTCAACCTCAACCAC+CGG + Chr7:86641599-86641618 MsG0780041105.01.T01:CDS 55.0%
TCTTTCTACACGTCCCGCAC+CGG - Chr7:86641689-86641708 None:intergenic 55.0%
TTCAGGCACGATCGACGGTA+CGG - Chr7:86641631-86641650 None:intergenic 55.0%
TTGTGGGTCCCACAGGTATC+CGG + Chr7:86641752-86641771 MsG0780041105.01.T01:CDS 55.0%
AACGCCGGTGGTTGAGGTTG+AGG - Chr7:86641606-86641625 None:intergenic 60.0%
ACGCCGGTGGTTGAGGTTGA+GGG - Chr7:86641605-86641624 None:intergenic 60.0%
AGGCGAAGCAGCGACGAACA+CGG - Chr7:86641562-86641581 None:intergenic 60.0%
ATCGACGGTACGGTCAACGC+CGG - Chr7:86641621-86641640 None:intergenic 60.0%
CCGGTTCAATCGAACCGGTG+CGG + Chr7:86641672-86641691 MsG0780041105.01.T01:CDS 60.0%
CGGTTCAATCGAACCGGTGC+GGG + Chr7:86641673-86641692 MsG0780041105.01.T01:CDS 60.0%
GGTGCGGGACGTGTAGAAAG+AGG + Chr7:86641688-86641707 MsG0780041105.01.T01:CDS 60.0%
GGTGGTTGAGGTTGAGGGAG+AGG - Chr7:86641600-86641619 None:intergenic 60.0%
!! CCGCACCGGTTCGATTGAAC+CGG - Chr7:86641675-86641694 None:intergenic 60.0%
ACGGTCAACGCCGGTGGTTG+AGG - Chr7:86641612-86641631 None:intergenic 65.0%
CGAAGCAGCGACGAACACGG+CGG - Chr7:86641559-86641578 None:intergenic 65.0%
CGTCGCTGCTTCGCCTGTTG+CGG + Chr7:86641566-86641585 MsG0780041105.01.T01:CDS 65.0%
GACGAACACGGCGGTGGAAG+CGG - Chr7:86641550-86641569 None:intergenic 65.0%
GCGCAGAGTCTGTGGCCTTC+TGG - Chr7:86641390-86641409 None:intergenic 65.0%
GGTGGAAGCGGATGCTGAGG+CGG - Chr7:86641538-86641557 None:intergenic 65.0%
AGCAGCGACGAACACGGCGG+TGG - Chr7:86641556-86641575 None:intergenic 70.0%
GACGGTACGGTCAACGCCGG+TGG - Chr7:86641618-86641637 None:intergenic 70.0%
GGAAGCGGATGCTGAGGCGG+AGG - Chr7:86641535-86641554 None:intergenic 70.0%
GGCGGTGGAAGCGGATGCTG+AGG - Chr7:86641541-86641560 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86641360 86641872 86641360 ID=MsG0780041105.01;Name=MsG0780041105.01
Chr7 mRNA 86641360 86641872 86641360 ID=MsG0780041105.01.T01;Parent=MsG0780041105.01;Name=MsG0780041105.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|170
Chr7 exon 86641360 86641872 86641360 ID=MsG0780041105.01.T01:exon:52042;Parent=MsG0780041105.01.T01
Chr7 CDS 86641360 86641872 86641360 ID=MsG0780041105.01.T01:cds;Parent=MsG0780041105.01.T01
Gene Sequence

>MsG0780041105.01.T01

ATGGCTGAAGAGCACCGATGTGAAACTCCAGAAGGCCACAGACTCTGCGCAAACAACTGCGGCTTCTTCGGCAGTTCAGCTACGATGAATCTCTGTTCAAAATGTTACAGAGACATCCATTTGAAGGAACAAGAACAAGCCAAAACTAAATCAACAATCGAAACGGCTCTTTCCTCCGCCTCAGCATCCGCTTCCACCGCCGTGTTCGTCGCTGCTTCGCCTGTTGCGGAAATCAAACCTCTCCCTCAACCTCAACCACCGGCGTTGACCGTACCGTCGATCGTGCCTGAAGCATCGGACAATTCACAAGGACCGGTTCAATCGAACCGGTGCGGGACGTGTAGAAAGAGGACCGGGTTAACCGGGTTCAAGTGTAGATGTGGAATAACGTTTTGTGGGTCCCACAGGTATCCGGAGAAACATGAGTGCGGTTTTGATTTCAAAGCAGTGGGGAGAGAGGAGATAGCGAAAGCGAATCCGGTGATCAAAGCTGATAAGCTCAGAAGAATTTGA

Protein sequence

>MsG0780041105.01.T01

MAEEHRCETPEGHRLCANNCGFFGSSATMNLCSKCYRDIHLKEQEQAKTKSTIETALSSASASASTAVFVAASPVAEIKPLPQPQPPALTVPSIVPEASDNSQGPVQSNRCGTCRKRTGLTGFKCRCGITFCGSHRYPEKHECGFDFKAVGREEIAKANPVIKADKLRRI*