AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011460.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011460.01.T01 MTR_2g104910 52.294 218 61 5 1 176 1 217 1.36e-53 171
MsG0280011460.01.T01 MTR_2g104910 50.000 206 60 5 13 176 2 206 4.12e-45 149
MsG0280011460.01.T01 MTR_4g006570 45.217 230 57 6 1 174 1 217 1.64e-41 140
MsG0280011460.01.T01 MTR_4g006570 43.779 217 55 6 12 174 30 233 1.23e-35 125
MsG0280011460.01.T01 MTR_4g006570 43.779 217 55 6 12 174 39 242 2.10e-35 125
MsG0280011460.01.T01 MTR_4g006570 43.779 217 55 6 12 174 44 247 2.27e-35 125
MsG0280011460.01.T01 MTR_4g006570 50.370 135 44 3 52 174 69 192 6.44e-29 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011460.01.T01 AT1G15880 42.791 215 77 4 5 176 6 217 7.60e-38 130

Find 48 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGTATACTTGTCTATTA+TGG 0.153434 2:+84293464 MsG0280011460.01.T01:three_prime_UTR
GTCTTCCAGCGTTCGACTTT+TGG 0.265092 2:+84292968 MsG0280011460.01.T01:CDS
ATTGTATACTTGTCTATTAT+GGG 0.266472 2:+84293465 MsG0280011460.01.T01:three_prime_UTR
ACCATTATACTTGCCCTTGT+TGG 0.314904 2:+84293351 MsG0280011460.01.T01:CDS
TGATCGAACAAGATTAGATT+TGG 0.341869 2:+84292537 MsG0280011460.01.T01:CDS
CCATTATACTTGCCCTTGTT+GGG 0.360182 2:+84293352 MsG0280011460.01.T01:CDS
CAATAAACATCATCTTTACT+TGG 0.366621 2:-84293413 None:intergenic
GTAATGCATTGTTTGATTGC+TGG 0.385471 2:+84293495 MsG0280011460.01.T01:three_prime_UTR
ATGTGTTACAATTCATAGTT+CGG 0.390079 2:+84293594 MsG0280011460.01.T01:three_prime_UTR
TGTTTATTGTGTTGGTAGCT+TGG 0.396853 2:+84293427 MsG0280011460.01.T01:CDS
CCCAACAAGGGCAAGTATAA+TGG 0.403543 2:-84293352 None:intergenic
TTGGGATTCCCTTCGCAAAC+AGG 0.411483 2:+84289256 MsG0280011460.01.T01:CDS
ATCCATGGAGGTTCAAAGTT+GGG 0.434741 2:+84289238 MsG0280011460.01.T01:exon
AGCTCAGTTGGATGAGCAAA+TGG 0.441426 2:+84291357 MsG0280011460.01.T01:CDS
TTCACAAATTAATCTTTACT+AGG 0.448626 2:-84293540 None:intergenic
GTTAGATGTATTTAAAATGC+AGG 0.453522 2:+84292441 MsG0280011460.01.T01:CDS
AACAAGATTAGATTTGGAAG+AGG 0.457292 2:+84292543 MsG0280011460.01.T01:CDS
TCAACGAAAGCTTCTACAAA+AGG 0.463049 2:+84291400 MsG0280011460.01.T01:CDS
TTTAAATACATCTAACAAGT+TGG 0.474443 2:-84292434 None:intergenic
AAAGATGATGTTTATTGTGT+TGG 0.505371 2:+84293419 MsG0280011460.01.T01:CDS
CATCCATGGAGGTTCAAAGT+TGG 0.506812 2:+84289237 MsG0280011460.01.T01:exon
GTCTGTGGCGTGTGAACAGA+CGG 0.519084 2:+84289183 MsG0280011460.01.T01:five_prime_UTR
TTGTATACTTGTCTATTATG+GGG 0.525930 2:+84293466 MsG0280011460.01.T01:three_prime_UTR
AAGAAAACTTGAAGCTCAGT+TGG 0.529386 2:+84291345 MsG0280011460.01.T01:CDS
GTTTATTCTTGTACTGCAGA+TGG 0.529780 2:+84292905 MsG0280011460.01.T01:CDS
CAACTTTGAACCTCCATGGA+TGG 0.538871 2:-84289236 None:intergenic
TTCAACAGCAATCATACCGT+TGG 0.538982 2:-84293031 None:intergenic
AGCGGTGTGGTCGTCGTCTG+TGG 0.544478 2:+84289168 MsG0280011460.01.T01:five_prime_UTR
ATCCCAACTTTGAACCTCCA+TGG 0.546573 2:-84289240 None:intergenic
TCAACAGCAATCATACCGTT+GGG 0.552335 2:-84293030 None:intergenic
ATGCATTGTTTGATTGCTGG+TGG 0.554948 2:+84293498 MsG0280011460.01.T01:three_prime_UTR
TGTATACTTGTCTATTATGG+GGG 0.555133 2:+84293467 MsG0280011460.01.T01:three_prime_UTR
GCTTAGCTCTAAGATTAGAC+CGG 0.556599 2:-84292477 None:intergenic
AATTGAAACCTGTTTGCGAA+GGG 0.560013 2:-84289264 None:intergenic
GAGAAATGAAAATGAAGAAG+CGG 0.564366 2:+84289150 MsG0280011460.01.T01:five_prime_UTR
ACAAGATTAGATTTGGAAGA+GGG 0.565048 2:+84292544 MsG0280011460.01.T01:CDS
AAATTGAAACCTGTTTGCGA+AGG 0.567814 2:-84289265 None:intergenic
CTAACCATCTCATCCATCCA+TGG 0.579099 2:+84289223 MsG0280011460.01.T01:exon
AAATGCATACGGACCCAACA+AGG 0.603761 2:-84293365 None:intergenic
TCCCACCAAAAGTCGAACGC+TGG 0.611928 2:-84292973 None:intergenic
CGTGAGCAGTCGCCTCCCAA+CGG 0.616247 2:+84293015 MsG0280011460.01.T01:CDS
ACCTCCATGGATGGATGAGA+TGG 0.623902 2:-84289227 None:intergenic
GTATACTTGTCTATTATGGG+GGG 0.624761 2:+84293468 MsG0280011460.01.T01:three_prime_UTR
AATGCATACGGACCCAACAA+GGG 0.630942 2:-84293364 None:intergenic
TCACAAGCACAAGCAACACT+CGG 0.631705 2:+84292938 MsG0280011460.01.T01:CDS
ATGAAAATGAAGAAGCGGTG+TGG 0.662471 2:+84289155 MsG0280011460.01.T01:five_prime_UTR
ACAGCAATCATACCGTTGGG+AGG 0.674625 2:-84293027 None:intergenic
ACCATCTCATCCATCCATGG+AGG 0.728976 2:+84289226 MsG0280011460.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAAAATGCATA+CGG - Chr2:84293379-84293398 None:intergenic 10.0%
!! ATATTTAATGATAAATGAAA+AGG - Chr2:84291755-84291774 None:intergenic 10.0%
!! GTTATAATAAATAATGTTAT+AGG - Chr2:84292246-84292265 None:intergenic 10.0%
!! GTTTAAATAAATATTGAATA+AGG + Chr2:84293054-84293073 MsG0280011460.01.T01:intron 10.0%
!! TATAATAAATAATGTTATAG+GGG - Chr2:84292244-84292263 None:intergenic 10.0%
!!! AAAGCTTATATATATTTTAT+AGG - Chr2:84292738-84292757 None:intergenic 10.0%
!!! ACAAAATATTTGAAATATAA+AGG - Chr2:84292391-84292410 None:intergenic 10.0%
!!! TCATTTTTTCAAATAATTAT+TGG - Chr2:84289597-84289616 None:intergenic 10.0%
!! ATTAATGATTTGATATATGA+TGG - Chr2:84290157-84290176 None:intergenic 15.0%
!! GTAACAGTTATAATAAATAA+TGG - Chr2:84292288-84292307 None:intergenic 15.0%
!!! AAAATTCAAAAGTATGATAT+GGG + Chr2:84289324-84289343 MsG0280011460.01.T01:intron 15.0%
!!! AGCATATTTATGTTTTTAAA+TGG + Chr2:84289111-84289130 MsG0280011460.01.T01:five_prime_UTR 15.0%
!!! TAAAATTCAAAAGTATGATA+TGG + Chr2:84289323-84289342 MsG0280011460.01.T01:intron 15.0%
!!! TTTTATTTATTTTCATCTAC+TGG + Chr2:84293386-84293405 MsG0280011460.01.T01:CDS 15.0%
!!! TTTTTTTTTTCTTTCTTGAA+GGG + Chr2:84291219-84291238 MsG0280011460.01.T01:intron 15.0%
!! AGGTCAAGTATAAATTTATT+AGG - Chr2:84291792-84291811 None:intergenic 20.0%
!! ATTGTATACTTGTCTATTAT+GGG + Chr2:84293465-84293484 MsG0280011460.01.T01:three_prime_UTR 20.0%
!! TATTGTATACTTGTCTATTA+TGG + Chr2:84293464-84293483 MsG0280011460.01.T01:three_prime_UTR 20.0%
!! TTCACAAATTAATCTTTACT+AGG - Chr2:84293543-84293562 None:intergenic 20.0%
!! TTTAAATACATCTAACAAGT+TGG - Chr2:84292437-84292456 None:intergenic 20.0%
!!! AATGGTTTTATATCGTAAAT+TGG + Chr2:84291375-84291394 MsG0280011460.01.T01:CDS 20.0%
!!! ATAAGCAAAAACTACTTTTA+AGG - Chr2:84290828-84290847 None:intergenic 20.0%
!!! ATCAAGTAATTTGTTGAATT+GGG + Chr2:84291196-84291215 MsG0280011460.01.T01:intron 20.0%
!!! CTATGTTCTTTTGTTATTTT+GGG + Chr2:84290474-84290493 MsG0280011460.01.T01:intron 20.0%
!!! GTTATCACTTTTTAATCTAA+TGG + Chr2:84289858-84289877 MsG0280011460.01.T01:intron 20.0%
!!! GTTTTTTTTTTCTTTCTTGA+AGG + Chr2:84291218-84291237 MsG0280011460.01.T01:intron 20.0%
!!! TATCAAGTAATTTGTTGAAT+TGG + Chr2:84291195-84291214 MsG0280011460.01.T01:intron 20.0%
!!! TCTATGTTCTTTTGTTATTT+TGG + Chr2:84290473-84290492 MsG0280011460.01.T01:intron 20.0%
!!! TCTTTTTATCTGTATTATGA+CGG - Chr2:84291013-84291032 None:intergenic 20.0%
!!! TTAATGTTTTATTTGGTTGT+CGG + Chr2:84289388-84289407 MsG0280011460.01.T01:intron 20.0%
! AAAAGACGACTTAAAGTTTA+TGG + Chr2:84291027-84291046 MsG0280011460.01.T01:intron 25.0%
! AAACTTCAAATGAACATTCA+AGG - Chr2:84292833-84292852 None:intergenic 25.0%
! AAAGATGATGTTTATTGTGT+TGG + Chr2:84293419-84293438 MsG0280011460.01.T01:CDS 25.0%
! AACCTATAAACCATATCAAA+AGG - Chr2:84289434-84289453 None:intergenic 25.0%
! ACCTATAAACCATATCAAAA+GGG - Chr2:84289433-84289452 None:intergenic 25.0%
! AGATCAAATAATCAAGATGA+CGG + Chr2:84290895-84290914 MsG0280011460.01.T01:intron 25.0%
! ATGTGTTACAATTCATAGTT+CGG + Chr2:84293594-84293613 MsG0280011460.01.T01:three_prime_UTR 25.0%
! ATTGGAATTGAAATAAAAGG+TGG - Chr2:84292180-84292199 None:intergenic 25.0%
! CAATAAACATCATCTTTACT+TGG - Chr2:84293416-84293435 None:intergenic 25.0%
! CATAATACTATATACACCAA+AGG - Chr2:84290419-84290438 None:intergenic 25.0%
! CATATTATATCATGTAGTTG+TGG + Chr2:84291630-84291649 MsG0280011460.01.T01:intron 25.0%
! GAAAAAATAATCCATTAGCT+TGG - Chr2:84292811-84292830 None:intergenic 25.0%
! GACATTGGAATTGAAATAAA+AGG - Chr2:84292183-84292202 None:intergenic 25.0%
! TAAAACTTGTGTAGTAGAAA+TGG - Chr2:84293239-84293258 None:intergenic 25.0%
! TAACAGTTTGAATTCTATAG+TGG - Chr2:84289842-84289861 None:intergenic 25.0%
! TGAGATCTTGATAAATTTCT+TGG - Chr2:84291556-84291575 None:intergenic 25.0%
! TTGTATACTTGTCTATTATG+GGG + Chr2:84293466-84293485 MsG0280011460.01.T01:three_prime_UTR 25.0%
! TTTCAATTCCAATGTCTTAT+AGG + Chr2:84292187-84292206 MsG0280011460.01.T01:intron 25.0%
!! AAGGTTTAGTTTAATAAGAG+TGG - Chr2:84292059-84292078 None:intergenic 25.0%
!! AGGTTTAGTTTAATAAGAGT+GGG - Chr2:84292058-84292077 None:intergenic 25.0%
!! AGTTTTTGTAGAATCTATCA+CGG + Chr2:84290680-84290699 MsG0280011460.01.T01:intron 25.0%
!! GTTAGATGTATTTAAAATGC+AGG + Chr2:84292441-84292460 MsG0280011460.01.T01:CDS 25.0%
!! TGATATATGATGGAACATTA+TGG - Chr2:84290147-84290166 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + Chr2:84290837-84290856 MsG0280011460.01.T01:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - Chr2:84290856-84290837 None:intergenic 25.0%
!!! ACAAAAACTTGCTTTTGAAA+AGG - Chr2:84290669-84290688 None:intergenic 25.0%
!!! ATGACATGATTTTGACATTT+TGG + Chr2:84289508-84289527 MsG0280011460.01.T01:intron 25.0%
!!! ATTATTTTAAATACTCCCTC+CGG - Chr2:84290859-84290878 None:intergenic 25.0%
!!! CAAAAACTTGCTTTTGAAAA+GGG - Chr2:84290668-84290687 None:intergenic 25.0%
!!! CACAAGTTTTATTTTCTCTA+TGG + Chr2:84293248-84293267 MsG0280011460.01.T01:intron 25.0%
!!! GCATAGTTCAAGTTAATTTT+AGG + Chr2:84291244-84291263 MsG0280011460.01.T01:intron 25.0%
!!! GTGCAATTTGTGTTTTAAAA+AGG - Chr2:84291919-84291938 None:intergenic 25.0%
!!! TAAGCAAAAAACTACTTTTG+AGG + Chr2:84290739-84290758 MsG0280011460.01.T01:intron 25.0%
!!! TATAGTTTTGAGTGATGAAT+TGG + Chr2:84289484-84289503 MsG0280011460.01.T01:intron 25.0%
!!! TGACATGATTTTGACATTTT+GGG + Chr2:84289509-84289528 MsG0280011460.01.T01:intron 25.0%
!!! TTAAAATGCAGGAGTTTTAT+CGG + Chr2:84292452-84292471 MsG0280011460.01.T01:CDS 25.0%
!!! TTATAGGTTTTGAGAATTTC+TGG + Chr2:84289445-84289464 MsG0280011460.01.T01:intron 25.0%
!!! TTCTGAAGTTTTTTTTTGTC+AGG + Chr2:84293198-84293217 MsG0280011460.01.T01:intron 25.0%
AACAAGATTAGATTTGGAAG+AGG + Chr2:84292543-84292562 MsG0280011460.01.T01:CDS 30.0%
AATAAAGCGAAAAAAGTCGA+TGG + Chr2:84293326-84293345 MsG0280011460.01.T01:CDS 30.0%
AATGACATCCTATAAGACAT+TGG - Chr2:84292198-84292217 None:intergenic 30.0%
ACAAGATTAGATTTGGAAGA+GGG + Chr2:84292544-84292563 MsG0280011460.01.T01:CDS 30.0%
ACCTGGATAAAAGATTGATT+CGG + Chr2:84290263-84290282 MsG0280011460.01.T01:intron 30.0%
ATAAAGAACCCTTTACATCA+GGG - Chr2:84291697-84291716 None:intergenic 30.0%
ATATGAAAGTCTAACATGAG+AGG + Chr2:84290010-84290029 MsG0280011460.01.T01:intron 30.0%
ATATGCTCACACATAATGAT+TGG - Chr2:84291615-84291634 None:intergenic 30.0%
CACAGAAAAACAAGTTATAC+AGG - Chr2:84291133-84291152 None:intergenic 30.0%
CATTGAATGAATGATGTATG+TGG + Chr2:84290764-84290783 MsG0280011460.01.T01:intron 30.0%
CTATGGTGTTTGTTTAATGA+CGG + Chr2:84293265-84293284 MsG0280011460.01.T01:intron 30.0%
GAGAAATGAAAATGAAGAAG+CGG + Chr2:84289150-84289169 MsG0280011460.01.T01:five_prime_UTR 30.0%
GCAAATGTCTGATATTAAGA+TGG - Chr2:84292789-84292808 None:intergenic 30.0%
GCATAATACATATACACCAA+AGG - Chr2:84290361-84290380 None:intergenic 30.0%
GCATATACTAATAGGAACAA+AGG - Chr2:84290397-84290416 None:intergenic 30.0%
TATGCTCACACATAATGATT+GGG - Chr2:84291614-84291633 None:intergenic 30.0%
TCAACTTCAGAATCAAAACA+GGG - Chr2:84291953-84291972 None:intergenic 30.0%
TCAATTTCTGCATATGCATT+CGG + Chr2:84290235-84290254 MsG0280011460.01.T01:intron 30.0%
TGATCGAACAAGATTAGATT+TGG + Chr2:84292537-84292556 MsG0280011460.01.T01:CDS 30.0%
TGCTTAGAAAACAAGTTTGT+AGG + Chr2:84289782-84289801 MsG0280011460.01.T01:intron 30.0%
TGTATACTTGTCTATTATGG+GGG + Chr2:84293467-84293486 MsG0280011460.01.T01:three_prime_UTR 30.0%
TGTTAGACTTTCATATGACA+AGG - Chr2:84290006-84290025 None:intergenic 30.0%
TTCCTATTAGTATATGCCTT+TGG + Chr2:84290400-84290419 MsG0280011460.01.T01:intron 30.0%
! ACCCTTTTGATATGGTTTAT+AGG + Chr2:84289429-84289448 MsG0280011460.01.T01:intron 30.0%
! ACTTTTTAATCTTCTCCATC+AGG + Chr2:84292310-84292329 MsG0280011460.01.T01:intron 30.0%
! ATATTCTGATGTTTTACGTG+TGG + Chr2:84289923-84289942 MsG0280011460.01.T01:intron 30.0%
! GGTTTAGTTTAATAAGAGTG+GGG - Chr2:84292057-84292076 None:intergenic 30.0%
! TTGTCTTTTTAACCTTTTGC+AGG + Chr2:84291321-84291340 MsG0280011460.01.T01:intron 30.0%
! TTTTATCAAGATTGTTCCAC+TGG + Chr2:84292671-84292690 MsG0280011460.01.T01:intron 30.0%
!! TTGACTAGTTTCTGATGATA+GGG + Chr2:84289718-84289737 MsG0280011460.01.T01:intron 30.0%
!! TTTGACTAGTTTCTGATGAT+AGG + Chr2:84289717-84289736 MsG0280011460.01.T01:intron 30.0%
!!! GAGAATTCTTTTGAAACTGA+AGG - Chr2:84291812-84291831 None:intergenic 30.0%
AAATTGAAACCTGTTTGCGA+AGG - Chr2:84289268-84289287 None:intergenic 35.0%
AACATGAGAGGATTCATTCT+CGG + Chr2:84290022-84290041 MsG0280011460.01.T01:intron 35.0%
AAGAAAACTTGAAGCTCAGT+TGG + Chr2:84291345-84291364 MsG0280011460.01.T01:CDS 35.0%
AATCATCTTGATCAGACTTC+TGG + Chr2:84290948-84290967 MsG0280011460.01.T01:intron 35.0%
AATTGAAACCTGTTTGCGAA+GGG - Chr2:84289267-84289286 None:intergenic 35.0%
AATTTATCAAGATCTCACCC+AGG + Chr2:84291559-84291578 MsG0280011460.01.T01:intron 35.0%
AGTCACCTAATTGTTCTAAC+TGG + Chr2:84292094-84292113 MsG0280011460.01.T01:intron 35.0%
ATGCTCACACATAATGATTG+GGG - Chr2:84291613-84291632 None:intergenic 35.0%
ATTGATGTACAAATCACTCG+AGG - Chr2:84290220-84290239 None:intergenic 35.0%
CAATGCCAGTTAGAACAATT+AGG - Chr2:84292102-84292121 None:intergenic 35.0%
CATAAAGAACCCTTTACATC+AGG - Chr2:84291698-84291717 None:intergenic 35.0%
CATATACAGCAACATAAGTG+GGG + Chr2:84290098-84290117 MsG0280011460.01.T01:intron 35.0%
CATTGAATGAGTGATGTATG+TGG - Chr2:84290803-84290822 None:intergenic 35.0%
GCATATACAGCAACATAAGT+GGG + Chr2:84290097-84290116 MsG0280011460.01.T01:intron 35.0%
GGGCTATAAATTCTGATCTT+TGG + Chr2:84289739-84289758 MsG0280011460.01.T01:intron 35.0%
GTAATGCATTGTTTGATTGC+TGG + Chr2:84293495-84293514 MsG0280011460.01.T01:three_prime_UTR 35.0%
GTATACTTGTCTATTATGGG+GGG + Chr2:84293468-84293487 MsG0280011460.01.T01:three_prime_UTR 35.0%
GTCAACTTCAGAATCAAAAC+AGG - Chr2:84291954-84291973 None:intergenic 35.0%
GTCCTATTAGTATATGCCTT+TGG + Chr2:84290342-84290361 MsG0280011460.01.T01:intron 35.0%
GTCTGATATTAAGATGGCAT+TGG - Chr2:84292783-84292802 None:intergenic 35.0%
GTTCTTTATGATAGCTGAGA+AGG + Chr2:84291708-84291727 MsG0280011460.01.T01:intron 35.0%
GTTTATTCTTGTACTGCAGA+TGG + Chr2:84292905-84292924 MsG0280011460.01.T01:CDS 35.0%
TATGGGTTTGTGTAATTCAG+AGG + Chr2:84289341-84289360 MsG0280011460.01.T01:intron 35.0%
TCAACGAAAGCTTCTACAAA+AGG + Chr2:84291400-84291419 MsG0280011460.01.T01:CDS 35.0%
TCATAACTCATACAGTCATC+AGG - Chr2:84290578-84290597 None:intergenic 35.0%
TGCATATACAGCAACATAAG+TGG + Chr2:84290096-84290115 MsG0280011460.01.T01:intron 35.0%
TGTTTCTTGCATCTTACAAC+CGG - Chr2:84290059-84290078 None:intergenic 35.0%
TTAATAAGAGTGGGGTAGAA+GGG - Chr2:84292049-84292068 None:intergenic 35.0%
TTTAATAAGAGTGGGGTAGA+AGG - Chr2:84292050-84292069 None:intergenic 35.0%
TTTATTTGGTTGTCGGTGAT+GGG + Chr2:84289395-84289414 MsG0280011460.01.T01:intron 35.0%
! ATATTAAGATGGCATTGGAG+CGG - Chr2:84292778-84292797 None:intergenic 35.0%
! CACCAAAGGCATATACTAAT+AGG - Chr2:84290347-84290366 None:intergenic 35.0%
! GAGTTTCTACCCTTTTGATA+TGG + Chr2:84289421-84289440 MsG0280011460.01.T01:intron 35.0%
! TCCGAATCAATCTTTTATCC+AGG - Chr2:84290267-84290286 None:intergenic 35.0%
! TCTTGTTAAACCTTTTCCCA+CGG + Chr2:84290443-84290462 MsG0280011460.01.T01:intron 35.0%
!! AGTTCTGAAAAACAGAGAAG+TGG - Chr2:84292006-84292025 None:intergenic 35.0%
!! ATCTGTATTATGACGGACTA+CGG - Chr2:84291006-84291025 None:intergenic 35.0%
!! TGACTAGTTTCTGATGATAG+GGG + Chr2:84289719-84289738 MsG0280011460.01.T01:intron 35.0%
!! TGTTTATTGTGTTGGTAGCT+TGG + Chr2:84293427-84293446 MsG0280011460.01.T01:CDS 35.0%
!! TTTTATTTGGTTGTCGGTGA+TGG + Chr2:84289394-84289413 MsG0280011460.01.T01:intron 35.0%
!!! TTGACATTTTGGGTTCATCT+AGG + Chr2:84289519-84289538 MsG0280011460.01.T01:intron 35.0%
AACTGTACTCCCTGATGTAA+AGG + Chr2:84291685-84291704 MsG0280011460.01.T01:intron 40.0%
ACAAGTAAACTCACAGATGC+AGG + Chr2:84291478-84291497 MsG0280011460.01.T01:intron 40.0%
ACATCACAAAGCAACAGAGT+AGG - Chr2:84292336-84292355 None:intergenic 40.0%
ACCATTATACTTGCCCTTGT+TGG + Chr2:84293351-84293370 MsG0280011460.01.T01:CDS 40.0%
ACTAAACCTTCTAACCTCTC+TGG + Chr2:84292069-84292088 MsG0280011460.01.T01:intron 40.0%
ACTGTACTCCCTGATGTAAA+GGG + Chr2:84291686-84291705 MsG0280011460.01.T01:intron 40.0%
ACTTCAGAATCAAAACAGGG+TGG - Chr2:84291950-84291969 None:intergenic 40.0%
AGATTAGATTTGGAAGAGGG+TGG + Chr2:84292547-84292566 MsG0280011460.01.T01:intron 40.0%
AGCTCATACGATGTAATACC+TGG - Chr2:84291580-84291599 None:intergenic 40.0%
ATCCATGGAGGTTCAAAGTT+GGG + Chr2:84289238-84289257 MsG0280011460.01.T01:exon 40.0%
ATGAAAATGAAGAAGCGGTG+TGG + Chr2:84289155-84289174 MsG0280011460.01.T01:five_prime_UTR 40.0%
ATGCATTGTTTGATTGCTGG+TGG + Chr2:84293498-84293517 MsG0280011460.01.T01:three_prime_UTR 40.0%
CCATTATACTTGCCCTTGTT+GGG + Chr2:84293352-84293371 MsG0280011460.01.T01:CDS 40.0%
GAAAGCTTCTACAAAAGGTG+AGG + Chr2:84291405-84291424 MsG0280011460.01.T01:intron 40.0%
GACTTTCATATGACAAGGTG+AGG - Chr2:84290001-84290020 None:intergenic 40.0%
GATCTATGAAGCACATACAC+TGG + Chr2:84289548-84289567 MsG0280011460.01.T01:intron 40.0%
GCTCATACGATGTAATACCT+GGG - Chr2:84291579-84291598 None:intergenic 40.0%
TCAACAGCAATCATACCGTT+GGG - Chr2:84293033-84293052 None:intergenic 40.0%
TCAGACATTTGCCAAGCTAA+TGG + Chr2:84292797-84292816 MsG0280011460.01.T01:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG + Chr2:84290841-84290860 MsG0280011460.01.T01:intron 40.0%
TGCTTATAATAGTGACCGGA+GGG - Chr2:84290860-84290841 None:intergenic 40.0%
TGTCTGACAGATCTAAGCTT+AGG - Chr2:84292414-84292433 None:intergenic 40.0%
TTATTTGGTTGTCGGTGATG+GGG + Chr2:84289396-84289415 MsG0280011460.01.T01:intron 40.0%
TTCAACAGCAATCATACCGT+TGG - Chr2:84293034-84293053 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + Chr2:84290840-84290859 MsG0280011460.01.T01:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG - Chr2:84290859-84290840 None:intergenic 40.0%
TTTCATATGACAAGGTGAGG+TGG - Chr2:84289998-84290017 None:intergenic 40.0%
TTTCTGATCATAGGTGTGTC+AGG - Chr2:84289663-84289682 None:intergenic 40.0%
! ACGGCTTTTAACAATGTTGC+AGG + Chr2:84293284-84293303 MsG0280011460.01.T01:intron 40.0%
! AGGGAGTACAGTTTGCAAAT+AGG - Chr2:84291678-84291697 None:intergenic 40.0%
! ATAATCAAGATGACGGCAGT+TGG + Chr2:84290902-84290921 MsG0280011460.01.T01:intron 40.0%
! ATGAGAGAGGAGTATAACAG+AGG + Chr2:84291842-84291861 MsG0280011460.01.T01:intron 40.0%
! ATGTTTTACGTGTGGTGGTA+TGG + Chr2:84289931-84289950 MsG0280011460.01.T01:intron 40.0%
! CAAGTTTTCTTGCCTGCAAA+AGG - Chr2:84291336-84291355 None:intergenic 40.0%
! CTCTTTTCTACAGATGAGAG+AGG + Chr2:84291829-84291848 MsG0280011460.01.T01:intron 40.0%
! GCACTGACATTTCTGATCAT+AGG - Chr2:84289672-84289691 None:intergenic 40.0%
! TGAGAGAGGAGTATAACAGA+GGG + Chr2:84291843-84291862 MsG0280011460.01.T01:intron 40.0%
! TTCTGATGTTTTACGTGTGG+TGG + Chr2:84289926-84289945 MsG0280011460.01.T01:intron 40.0%
!! CTCTGTTGCTTTGTGATGTT+AGG + Chr2:84292337-84292356 MsG0280011460.01.T01:intron 40.0%
!! GCTTAGCTCTAAGATTAGAC+CGG - Chr2:84292480-84292499 None:intergenic 40.0%
!! GGAGTCTTTCTGAAGCTAAA+AGG - Chr2:84291898-84291917 None:intergenic 40.0%
AAACTCACAGATGCAGGCTT+GGG + Chr2:84291484-84291503 MsG0280011460.01.T01:intron 45.0%
AAATGCATACGGACCCAACA+AGG - Chr2:84293368-84293387 None:intergenic 45.0%
AATGCATACGGACCCAACAA+GGG - Chr2:84293367-84293386 None:intergenic 45.0%
ACACGTGTCTATGACACTTG+TGG + Chr2:84289634-84289653 MsG0280011460.01.T01:intron 45.0%
ACTTGTCTATTATGGGGGGA+GGG + Chr2:84293472-84293491 MsG0280011460.01.T01:three_prime_UTR 45.0%
AGAAGTGGATCAACTGCAAC+GGG - Chr2:84291991-84292010 None:intergenic 45.0%
AGCTCAGTTGGATGAGCAAA+TGG + Chr2:84291357-84291376 MsG0280011460.01.T01:CDS 45.0%
ATATGCATTCGGCGATAACC+TGG + Chr2:84290246-84290265 MsG0280011460.01.T01:intron 45.0%
ATCCCAACTTTGAACCTCCA+TGG - Chr2:84289243-84289262 None:intergenic 45.0%
ATGAGAGGATTCATTCTCGG+TGG + Chr2:84290025-84290044 MsG0280011460.01.T01:intron 45.0%
CAACTTTGAACCTCCATGGA+TGG - Chr2:84289239-84289258 None:intergenic 45.0%
CATCCATGGAGGTTCAAAGT+TGG + Chr2:84289237-84289256 MsG0280011460.01.T01:exon 45.0%
CCCAACAAGGGCAAGTATAA+TGG - Chr2:84293355-84293374 None:intergenic 45.0%
CTAACCATCTCATCCATCCA+TGG + Chr2:84289223-84289242 MsG0280011460.01.T01:exon 45.0%
GAATTCTATAGTGGCTGCAC+AGG - Chr2:84289833-84289852 None:intergenic 45.0%
GAGAAGTGGATCAACTGCAA+CGG - Chr2:84291992-84292011 None:intergenic 45.0%
GTAGAATCTATCACGGCTTC+TGG + Chr2:84290687-84290706 MsG0280011460.01.T01:intron 45.0%
TAAACTCACAGATGCAGGCT+TGG + Chr2:84291483-84291502 MsG0280011460.01.T01:intron 45.0%
TAACAACCTGTCCTGTACTG+CGG - Chr2:84292618-84292637 None:intergenic 45.0%
TAATAAGTGACGCACTCCAG+TGG - Chr2:84292690-84292709 None:intergenic 45.0%
TACTTGTCTATTATGGGGGG+AGG + Chr2:84293471-84293490 MsG0280011460.01.T01:three_prime_UTR 45.0%
TCAAAACAGGGTGGACGAAT+AGG - Chr2:84291941-84291960 None:intergenic 45.0%
TCACAAGCACAAGCAACACT+CGG + Chr2:84292938-84292957 MsG0280011460.01.T01:CDS 45.0%
TGAGAGGATTCATTCTCGGT+GGG + Chr2:84290026-84290045 MsG0280011460.01.T01:intron 45.0%
TGGCTAATACTTACTCCCTC+CGG + Chr2:84290707-84290726 MsG0280011460.01.T01:intron 45.0%
! AAACCTTTTCCCACGGCATA+GGG + Chr2:84290450-84290469 MsG0280011460.01.T01:intron 45.0%
! AATTAGGTGACTAGCCAGAG+AGG - Chr2:84292086-84292105 None:intergenic 45.0%
! GAGAGAGGAGTATAACAGAG+GGG + Chr2:84291844-84291863 MsG0280011460.01.T01:intron 45.0%
! TAAACCTTTTCCCACGGCAT+AGG + Chr2:84290449-84290468 MsG0280011460.01.T01:intron 45.0%
! TGATGGATGCACGCTCTTTA+AGG - Chr2:84292594-84292613 None:intergenic 45.0%
!! TTATAATAAATAATGTTATA+GGG - Chr2:84292245-84292264 None:intergenic 5.0%
!! TTATTATTTACTTATATATT+TGG + Chr2:84292704-84292723 MsG0280011460.01.T01:intron 5.0%
!!! AATATATTTTTTTGTTTTTT+AGG + Chr2:84291277-84291296 MsG0280011460.01.T01:intron 5.0%
!!! TATTTTTTTAATGTTTTATT+TGG + Chr2:84289381-84289400 MsG0280011460.01.T01:intron 5.0%
AAACAGAACAACCCCAGCTG+TGG - Chr2:84292875-84292894 None:intergenic 50.0%
AACCATTTCAGACCCACCAG+AGG - Chr2:84291513-84291532 None:intergenic 50.0%
AAGCAACAGAGTAGGCCTGA+TGG - Chr2:84292328-84292347 None:intergenic 50.0%
ACAGATGTCTGATCCACAGC+TGG + Chr2:84292859-84292878 MsG0280011460.01.T01:intron 50.0%
ACAGCAATCATACCGTTGGG+AGG - Chr2:84293030-84293049 None:intergenic 50.0%
ACCATCTCATCCATCCATGG+AGG + Chr2:84289226-84289245 MsG0280011460.01.T01:exon 50.0%
ACCTCCATGGATGGATGAGA+TGG - Chr2:84289230-84289249 None:intergenic 50.0%
AGAACATAGACCCTATGCCG+TGG - Chr2:84290463-84290482 None:intergenic 50.0%
AGATGTCTGATCCACAGCTG+GGG + Chr2:84292861-84292880 MsG0280011460.01.T01:intron 50.0%
ATTCTCGGTGGGTGACTTAC+CGG + Chr2:84290037-84290056 MsG0280011460.01.T01:intron 50.0%
CAGATGTCTGATCCACAGCT+GGG + Chr2:84292860-84292879 MsG0280011460.01.T01:intron 50.0%
CATAAGTGGGGTTGACTGCA+TGG + Chr2:84290110-84290129 MsG0280011460.01.T01:intron 50.0%
CTTGTCTATTATGGGGGGAG+GGG + Chr2:84293473-84293492 MsG0280011460.01.T01:three_prime_UTR 50.0%
GAACATAGACCCTATGCCGT+GGG - Chr2:84290462-84290481 None:intergenic 50.0%
GTACAAATCACTCGAGGCAG+AGG - Chr2:84290214-84290233 None:intergenic 50.0%
GTGTGGTGGTATGGTACAGA+CGG + Chr2:84289940-84289959 MsG0280011460.01.T01:intron 50.0%
TGAGGCAACAGAGAGTGATC+TGG + Chr2:84291423-84291442 MsG0280011460.01.T01:intron 50.0%
TTGGGATTCCCTTCGCAAAC+AGG + Chr2:84289256-84289275 MsG0280011460.01.T01:CDS 50.0%
! CAGAGAGTGATCTGGAGTCT+TGG + Chr2:84291431-84291450 MsG0280011460.01.T01:intron 50.0%
! GACTAGCCAGAGAGGTTAGA+AGG - Chr2:84292078-84292097 None:intergenic 50.0%
! GTCTTCCAGCGTTCGACTTT+TGG + Chr2:84292968-84292987 MsG0280011460.01.T01:CDS 50.0%
! TCCAGCGTTCGACTTTTGGT+GGG + Chr2:84292972-84292991 MsG0280011460.01.T01:CDS 50.0%
! TGGATGCACGCTCTTTAAGG+AGG - Chr2:84292591-84292610 None:intergenic 50.0%
! TTCCAGCGTTCGACTTTTGG+TGG + Chr2:84292971-84292990 MsG0280011460.01.T01:CDS 50.0%
!!! TGCAGGAGTTTTATCGGCTC+CGG + Chr2:84292458-84292477 MsG0280011460.01.T01:CDS 50.0%
ACTCGAGGCAGAGGAAGACA+TGG - Chr2:84290205-84290224 None:intergenic 55.0%
AGACCCTATGCCGTGGGAAA+AGG - Chr2:84290456-84290475 None:intergenic 55.0%
CAACTGCAACGGGAGATCGA+CGG - Chr2:84291981-84292000 None:intergenic 55.0%
GTCTGTGGCGTGTGAACAGA+CGG + Chr2:84289183-84289202 MsG0280011460.01.T01:five_prime_UTR 55.0%
TCCCACCAAAAGTCGAACGC+TGG - Chr2:84292976-84292995 None:intergenic 55.0%
! GTCCTCTGGTGGGTCTGAAA+TGG + Chr2:84291508-84291527 MsG0280011460.01.T01:intron 55.0%
! TGGCTCTGCAGTGACTGCTT+TGG + Chr2:84289878-84289897 MsG0280011460.01.T01:intron 55.0%
AGGCTTGGGTGTCCTCTGGT+GGG + Chr2:84291498-84291517 MsG0280011460.01.T01:intron 60.0%
ATGCAGGCTTGGGTGTCCTC+TGG + Chr2:84291494-84291513 MsG0280011460.01.T01:intron 60.0%
CATCAGCCGCAGTACAGGAC+AGG + Chr2:84292609-84292628 MsG0280011460.01.T01:intron 60.0%
CTGTCCTGTACTGCGGCTGA+TGG - Chr2:84292611-84292630 None:intergenic 60.0%
GCATCCATCAGCCGCAGTAC+AGG + Chr2:84292604-84292623 MsG0280011460.01.T01:intron 60.0%
AGCGGTGTGGTCGTCGTCTG+TGG + Chr2:84289168-84289187 MsG0280011460.01.T01:five_prime_UTR 65.0%
CAGGCTTGGGTGTCCTCTGG+TGG + Chr2:84291497-84291516 MsG0280011460.01.T01:intron 65.0%
CGTGAGCAGTCGCCTCCCAA+CGG + Chr2:84293015-84293034 MsG0280011460.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 84289110 84293651 84289110 ID=MsG0280011460.01;Name=MsG0280011460.01
Chr2 mRNA 84289110 84293651 84289110 ID=MsG0280011460.01.T01;Parent=MsG0280011460.01;Name=MsG0280011460.01.T01;_AED=0.49;_eAED=0.61;_QI=132|0.25|0.4|1|0|0|5|196|194
Chr2 exon 84289110 84289277 84289110 ID=MsG0280011460.01.T01:exon:12926;Parent=MsG0280011460.01.T01
Chr2 exon 84291343 84291421 84291343 ID=MsG0280011460.01.T01:exon:12927;Parent=MsG0280011460.01.T01
Chr2 exon 84292422 84292565 84292422 ID=MsG0280011460.01.T01:exon:12928;Parent=MsG0280011460.01.T01
Chr2 exon 84292878 84293053 84292878 ID=MsG0280011460.01.T01:exon:12929;Parent=MsG0280011460.01.T01
Chr2 exon 84293306 84293651 84293306 ID=MsG0280011460.01.T01:exon:12930;Parent=MsG0280011460.01.T01
Chr2 five_prime_UTR 84289110 84289241 84289110 ID=MsG0280011460.01.T01:five_prime_utr;Parent=MsG0280011460.01.T01
Chr2 CDS 84289242 84289277 84289242 ID=MsG0280011460.01.T01:cds;Parent=MsG0280011460.01.T01
Chr2 CDS 84291343 84291421 84291343 ID=MsG0280011460.01.T01:cds;Parent=MsG0280011460.01.T01
Chr2 CDS 84292422 84292565 84292422 ID=MsG0280011460.01.T01:cds;Parent=MsG0280011460.01.T01
Chr2 CDS 84292878 84293053 84292878 ID=MsG0280011460.01.T01:cds;Parent=MsG0280011460.01.T01
Chr2 CDS 84293306 84293455 84293306 ID=MsG0280011460.01.T01:cds;Parent=MsG0280011460.01.T01
Chr2 three_prime_UTR 84293456 84293651 84293456 ID=MsG0280011460.01.T01:three_prime_utr;Parent=MsG0280011460.01.T01
Gene Sequence

>MsG0280011460.01.T01

ATGGAGGTTCAAAGTTGGGATTCCCTTCGCAAACAGGCAAGAAAACTTGAAGCTCAGTTGGATGAGCAAATGGTTTTATATCGTAAATTGGTTTCAACGAAAGCTTCTACAAAAGATCTGTCAGACACCAACTTGTTAGATGTATTTAAAATGCAGGAGTTTTATCGGCTCCGGTCTAATCTTAGAGCTAAGCAAGAACACGCTTCACTACTTGATGATTTTAAAGAGCTTGATCGAACAAGATTAGATTTGGAAGAGGCTGGGGTTGTTCTGTTTAATATACTGTGTTTATTCTTGTACTGCAGATGGACACTGTTATTCACAAGCACAAGCAACACTCGGTGCACTAGTCTTCCAGCGTTCGACTTTTGGTGGGATCAATTCAAAGCTCAGCAACGTGAGCAGTCGCCTCCCAACGGTATGATTGCTGTTGAAGTAAATACTATTCTGTCAGCAATAAAGCGAAAAAAGTCGATGGATACCATTATACTTGCCCTTGTTGGGTCCGTATGCATTTTTATTTATTTTCATCTACTGGCTAACCAAGTAAAGATGATGTTTATTGTGTTGGTAGCTTGGATTTAA
Protein sequence

>MsG0280011460.01.T01

MEVQSWDSLRKQARKLEAQLDEQMVLYRKLVSTKASTKDLSDTNLLDVFKMQEFYRLRSNLRAKQEHASLLDDFKELDRTRLDLEEAGVVLFNILCLFLYCRWTLLFTSTSNTRCTSLPAFDFWWDQFKAQQREQSPPNGMIAVEVNTILSAIKRKKSMDTIILALVGSVCIFIYFHLLANQVKMMFIVLVAWI*