AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031096.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031096.01.T01 MTR_7g062720 93.939 132 8 0 1 132 1 132 1.69e-86 258
MsG0680031096.01.T01 MTR_4g037355 70.769 130 24 3 151 280 1 116 3.55e-54 175
MsG0680031096.01.T01 MTR_5g061150 63.333 120 44 0 27 146 17 136 2.56e-47 157
MsG0680031096.01.T01 MTR_4g112730 75.000 64 16 0 138 201 1 64 2.72e-26 100
MsG0680031096.01.T01 MTR_1g036690 61.628 86 22 3 273 357 5 80 1.25e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCCCTTAACATTTC+TGG 0.105015 6:-14250445 MsG0680031096.01.T01:CDS
GCATCTCAACACAATCATTT+TGG 0.131367 6:-14249869 MsG0680031096.01.T01:CDS
TTCCACTTCTGGTGGGAAAA+TGG 0.172180 6:+14249734 None:intergenic
TCTTAAGTGCTTCATTTATA+TGG 0.214395 6:+14250293 None:intergenic
ATCCAATCATATCTTGCTTT+TGG 0.220117 6:+14250187 None:intergenic
GATGCTGATTATGATATTTA+TGG 0.234910 6:-14249515 MsG0680031096.01.T01:CDS
AATACTACTCCTTTGGATAT+TGG 0.253196 6:-14249485 MsG0680031096.01.T01:CDS
TCCACTTCTGGTGGGAAAAT+GGG 0.275118 6:+14249735 None:intergenic
TTTGTATACTTCAGAATTAT+AGG 0.281074 6:+14250129 None:intergenic
TGTTGAGATGCATTCACTTC+AGG 0.293409 6:+14249881 None:intergenic
TGCCATAAAAGAAGGAAATA+AGG 0.335057 6:-14250359 MsG0680031096.01.T01:CDS
AGTTGTTTCCAGAAATGTTA+AGG 0.345031 6:+14250437 None:intergenic
CACCAGCAGGGCGAGCTTCA+TGG 0.368055 6:+14249915 None:intergenic
CGCGGTCGTGGTCGTGGTCG+TGG 0.386944 6:-14249815 MsG0680031096.01.T01:CDS
ATTGATCACCTTGTTGAAGA+TGG 0.391237 6:-14249434 MsG0680031096.01.T01:CDS
GTTGTTTCCAGAAATGTTAA+GGG 0.399743 6:+14250438 None:intergenic
TAATTTAATTTCTTGTCTCC+TGG 0.400386 6:-14249972 MsG0680031096.01.T01:CDS
CTGGAAACAACTATTTGACT+TGG 0.414379 6:-14250426 MsG0680031096.01.T01:CDS
TACTTAGCTGCTGAAGGAAA+TGG 0.418999 6:-14250385 MsG0680031096.01.T01:CDS
GTTGAGATGCATTCACTTCA+GGG 0.423894 6:+14249882 None:intergenic
CGCGGTCGCGGTCGTGGTCG+TGG 0.429172 6:-14249821 MsG0680031096.01.T01:CDS
TAAAGGTCAACCAAATGCTT+TGG 0.429910 6:+14249602 None:intergenic
GGTCATGCCCACAATCCTAA+TGG 0.437132 6:-14249770 MsG0680031096.01.T01:CDS
CAACTATTTGACTTGGGCCG+TGG 0.437626 6:-14250419 MsG0680031096.01.T01:CDS
AACCATGAAGCTCGCCCTGC+TGG 0.438159 6:-14249917 MsG0680031096.01.T01:CDS
TGACATGAATACTACTCCTT+TGG 0.440582 6:-14249492 MsG0680031096.01.T01:CDS
GGGAATGGAGCTACACCAGC+AGG 0.445287 6:+14249902 None:intergenic
CGTGGTCGCGGTCGCGGTCG+TGG 0.450743 6:-14249827 MsG0680031096.01.T01:CDS
CGTGGTCGTGGTCGCGGTCG+CGG 0.452026 6:-14249833 MsG0680031096.01.T01:CDS
CCACTTCTGGTGGGAAAATG+GGG 0.456004 6:+14249736 None:intergenic
TCTCTCAAAACTTCACTTTG+AGG 0.457299 6:-14250461 MsG0680031096.01.T01:CDS
ATGTCGTACTCCAAAGCATT+TGG 0.465557 6:-14249612 MsG0680031096.01.T01:CDS
TTTGGGAGCGCTCGTGGTCG+TGG 0.467855 6:-14249851 MsG0680031096.01.T01:CDS
TCCCAAAAGCAAGATATGAT+TGG 0.468237 6:-14250189 MsG0680031096.01.T01:CDS
TGAAAGATCCACTTGTGCTC+TGG 0.468533 6:-14250255 MsG0680031096.01.T01:CDS
AGCGCTCGTGGTCGTGGTCG+TGG 0.471808 6:-14249845 MsG0680031096.01.T01:CDS
ACTCATTATGCTTATAAAGA+TGG 0.483040 6:-14249542 MsG0680031096.01.T01:CDS
TTGTTGTTGCAAGAGCATAT+TGG 0.488960 6:+14250024 None:intergenic
CGTGGTCGTGGTCGTGGTCG+CGG 0.491270 6:-14249839 MsG0680031096.01.T01:CDS
TGCAACAACAATACCGTGAA+AGG 0.491455 6:-14250012 MsG0680031096.01.T01:CDS
CATTGTTCTGTTCAGCAACC+AGG 0.503364 6:+14249954 None:intergenic
AAAGTATCTGTTATCGATGT+GGG 0.505636 6:-14249658 MsG0680031096.01.T01:CDS
GCAACAACAATACCGTGAAA+GGG 0.512293 6:-14250011 MsG0680031096.01.T01:CDS
AGTTTATTCCAGAGCACAAG+TGG 0.512360 6:+14250247 None:intergenic
GATGCATTCACTTCAGGGAA+TGG 0.514758 6:+14249887 None:intergenic
GAAAAGAATGAAAATGAGAA+GGG 0.529787 6:-14249704 MsG0680031096.01.T01:CDS
TGGAAACAACTATTTGACTT+GGG 0.534823 6:-14250425 MsG0680031096.01.T01:CDS
TGAAAAGAATGAAAATGAGA+AGG 0.541748 6:-14249705 MsG0680031096.01.T01:CDS
AATATGCATGGTCGTGGTCG+TGG 0.544688 6:-14249791 MsG0680031096.01.T01:CDS
AATGGAGATGCCATAAAAGA+AGG 0.547076 6:-14250367 MsG0680031096.01.T01:CDS
GAGCATATTGGATGCATGAA+AGG 0.547906 6:+14250036 None:intergenic
AAGAATGAAAATGAGAAGGG+TGG 0.573649 6:-14249701 MsG0680031096.01.T01:CDS
GAAAGTATCTGTTATCGATG+TGG 0.574435 6:-14249659 MsG0680031096.01.T01:CDS
GAAATGTACTTAGCTGCTGA+AGG 0.580070 6:-14250391 MsG0680031096.01.T01:CDS
ACTTCTCAATTAGAATTGTG+TGG 0.587587 6:-14250103 MsG0680031096.01.T01:CDS
CAACAACAATACCGTGAAAG+GGG 0.610582 6:-14250010 MsG0680031096.01.T01:CDS
TAAGTACATTTCAGTATCCA+CGG 0.611101 6:+14250402 None:intergenic
CTTCATTTATATGGCGACGA+AGG 0.612750 6:+14250302 None:intergenic
AAGAAATCACCAATATCCAA+AGG 0.617484 6:+14249476 None:intergenic
AGTATCTGTTATCGATGTGG+GGG 0.620042 6:-14249656 MsG0680031096.01.T01:CDS
CGTGGAAATATGCATGGTCG+TGG 0.624916 6:-14249797 MsG0680031096.01.T01:CDS
AAGTATCTGTTATCGATGTG+GGG 0.635242 6:-14249657 MsG0680031096.01.T01:CDS
TCACGATTCCATCTTCAACA+AGG 0.635824 6:+14249426 None:intergenic
CGTGGTCGTGGAAATATGCA+TGG 0.646225 6:-14249803 MsG0680031096.01.T01:CDS
GGAATGGAGCTACACCAGCA+GGG 0.651331 6:+14249903 None:intergenic
CAAGTGGATCTTTCACAGTG+AGG 0.718603 6:+14250263 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAAAACAAAGGAAAAA+TGG - Chr6:14250317-14250336 MsG0680031096.01.T01:CDS 20.0%
!! TTTGTATACTTCAGAATTAT+AGG + Chr6:14249761-14249780 None:intergenic 20.0%
! ACTCATTATGCTTATAAAGA+TGG - Chr6:14250345-14250364 MsG0680031096.01.T01:CDS 25.0%
! GAAAAGAATGAAAATGAGAA+GGG - Chr6:14250183-14250202 MsG0680031096.01.T01:CDS 25.0%
! TCTTAAGTGCTTCATTTATA+TGG + Chr6:14249597-14249616 None:intergenic 25.0%
! TGAAAAGAATGAAAATGAGA+AGG - Chr6:14250182-14250201 MsG0680031096.01.T01:CDS 25.0%
!! GATGCTGATTATGATATTTA+TGG - Chr6:14250372-14250391 MsG0680031096.01.T01:CDS 25.0%
!! TAATTTAATTTCTTGTCTCC+TGG - Chr6:14249915-14249934 MsG0680031096.01.T01:CDS 25.0%
!!! TTTCATTATTTTTCCACTTC+TGG + Chr6:14250167-14250186 None:intergenic 25.0%
!!! TTTTTAGTGATTGCTGATAA+AGG + Chr6:14250305-14250324 None:intergenic 25.0%
AAGAAATCACCAATATCCAA+AGG + Chr6:14250414-14250433 None:intergenic 30.0%
AATACTACTCCTTTGGATAT+TGG - Chr6:14250402-14250421 MsG0680031096.01.T01:CDS 30.0%
ACTTCTCAATTAGAATTGTG+TGG - Chr6:14249784-14249803 MsG0680031096.01.T01:CDS 30.0%
CAGCAATCACTAAAAAACAA+AGG - Chr6:14250309-14250328 MsG0680031096.01.T01:CDS 30.0%
TAAGTACATTTCAGTATCCA+CGG + Chr6:14249488-14249507 None:intergenic 30.0%
TGCCATAAAAGAAGGAAATA+AGG - Chr6:14249528-14249547 MsG0680031096.01.T01:CDS 30.0%
TGGTGAAAAAATAACAGATG+CGG - Chr6:14249804-14249823 MsG0680031096.01.T01:CDS 30.0%
! AAAGTATCTGTTATCGATGT+GGG - Chr6:14250229-14250248 MsG0680031096.01.T01:CDS 30.0%
! AGTTGTTTCCAGAAATGTTA+AGG + Chr6:14249453-14249472 None:intergenic 30.0%
! ATCCAATCATATCTTGCTTT+TGG + Chr6:14249703-14249722 None:intergenic 30.0%
! CATCTCAACACAATCATTTT+GGG - Chr6:14250019-14250038 MsG0680031096.01.T01:CDS 30.0%
! GAATTTTCCATTAGGATTGT+GGG + Chr6:14250127-14250146 None:intergenic 30.0%
! GTTGTTTCCAGAAATGTTAA+GGG + Chr6:14249452-14249471 None:intergenic 30.0%
! TGAATTTTCCATTAGGATTG+TGG + Chr6:14250128-14250147 None:intergenic 30.0%
! TGGAAACAACTATTTGACTT+GGG - Chr6:14249462-14249481 MsG0680031096.01.T01:CDS 30.0%
!! ATTATTTTTCCACTTCTGGT+GGG + Chr6:14250163-14250182 None:intergenic 30.0%
!! TCCAATCATATCTTGCTTTT+GGG + Chr6:14249702-14249721 None:intergenic 30.0%
!!! ATTTTTGAAATCCCCTTTCA+CGG + Chr6:14249891-14249910 None:intergenic 30.0%
!!! TGCCTTATTTCCTTCTTTTA+TGG + Chr6:14249533-14249552 None:intergenic 30.0%
AAGAATGAAAATGAGAAGGG+TGG - Chr6:14250186-14250205 MsG0680031096.01.T01:CDS 35.0%
AAGTATCTGTTATCGATGTG+GGG - Chr6:14250230-14250249 MsG0680031096.01.T01:CDS 35.0%
AATGGAGATGCCATAAAAGA+AGG - Chr6:14249520-14249539 MsG0680031096.01.T01:CDS 35.0%
ATTGATCACCTTGTTGAAGA+TGG - Chr6:14250453-14250472 MsG0680031096.01.T01:CDS 35.0%
CTGGAAACAACTATTTGACT+TGG - Chr6:14249461-14249480 MsG0680031096.01.T01:CDS 35.0%
TAAAGGTCAACCAAATGCTT+TGG + Chr6:14250288-14250307 None:intergenic 35.0%
TCCCAAAAGCAAGATATGAT+TGG - Chr6:14249698-14249717 MsG0680031096.01.T01:CDS 35.0%
TCTCTCAAAACTTCACTTTG+AGG - Chr6:14249426-14249445 MsG0680031096.01.T01:CDS 35.0%
TGACATGAATACTACTCCTT+TGG - Chr6:14250395-14250414 MsG0680031096.01.T01:CDS 35.0%
! GCATCTCAACACAATCATTT+TGG - Chr6:14250018-14250037 MsG0680031096.01.T01:CDS 35.0%
! TTGTTGTTGCAAGAGCATAT+TGG + Chr6:14249866-14249885 None:intergenic 35.0%
!! AATCATATCTTGCTTTTGGG+AGG + Chr6:14249699-14249718 None:intergenic 35.0%
!! CATTATTTTTCCACTTCTGG+TGG + Chr6:14250164-14250183 None:intergenic 35.0%
!! GAAAGTATCTGTTATCGATG+TGG - Chr6:14250228-14250247 MsG0680031096.01.T01:CDS 35.0%
!!! TGCTTTTGCTTTTTGTTGCT+CGG + Chr6:14249557-14249576 None:intergenic 35.0%
AGTATCTGTTATCGATGTGG+GGG - Chr6:14250231-14250250 MsG0680031096.01.T01:CDS 40.0%
AGTTTATTCCAGAGCACAAG+TGG + Chr6:14249643-14249662 None:intergenic 40.0%
CAACAACAATACCGTGAAAG+GGG - Chr6:14249877-14249896 MsG0680031096.01.T01:CDS 40.0%
CTTCATTTATATGGCGACGA+AGG + Chr6:14249588-14249607 None:intergenic 40.0%
GAAATGTACTTAGCTGCTGA+AGG - Chr6:14249496-14249515 MsG0680031096.01.T01:CDS 40.0%
GAGCATATTGGATGCATGAA+AGG + Chr6:14249854-14249873 None:intergenic 40.0%
GCAACAACAATACCGTGAAA+GGG - Chr6:14249876-14249895 MsG0680031096.01.T01:CDS 40.0%
GTTGAGATGCATTCACTTCA+GGG + Chr6:14250008-14250027 None:intergenic 40.0%
TACTTAGCTGCTGAAGGAAA+TGG - Chr6:14249502-14249521 MsG0680031096.01.T01:CDS 40.0%
TCACGATTCCATCTTCAACA+AGG + Chr6:14250464-14250483 None:intergenic 40.0%
TGCAACAACAATACCGTGAA+AGG - Chr6:14249875-14249894 MsG0680031096.01.T01:CDS 40.0%
TGTTGAGATGCATTCACTTC+AGG + Chr6:14250009-14250028 None:intergenic 40.0%
! ATGTCGTACTCCAAAGCATT+TGG - Chr6:14250275-14250294 MsG0680031096.01.T01:CDS 40.0%
! TTTGAGGCCCTTAACATTTC+TGG - Chr6:14249442-14249461 MsG0680031096.01.T01:CDS 40.0%
!! TCTTGCTTTTGGGAGGATAA+TGG + Chr6:14249692-14249711 None:intergenic 40.0%
!!! GGGGTTCTGAATTTTCCATT+AGG + Chr6:14250135-14250154 None:intergenic 40.0%
CAAGTGGATCTTTCACAGTG+AGG + Chr6:14249627-14249646 None:intergenic 45.0%
CATTGTTCTGTTCAGCAACC+AGG + Chr6:14249936-14249955 None:intergenic 45.0%
GATGCATTCACTTCAGGGAA+TGG + Chr6:14250003-14250022 None:intergenic 45.0%
TCCACTTCTGGTGGGAAAAT+GGG + Chr6:14250155-14250174 None:intergenic 45.0%
TGAAAGATCCACTTGTGCTC+TGG - Chr6:14249632-14249651 MsG0680031096.01.T01:CDS 45.0%
TGTTATCGATGTGGGGGAAA+AGG - Chr6:14250237-14250256 MsG0680031096.01.T01:CDS 45.0%
TTCCACTTCTGGTGGGAAAA+TGG + Chr6:14250156-14250175 None:intergenic 45.0%
AATATGCATGGTCGTGGTCG+TGG - Chr6:14250096-14250115 MsG0680031096.01.T01:CDS 50.0%
CCACTTCTGGTGGGAAAATG+GGG + Chr6:14250154-14250173 None:intergenic 50.0%
CGTGGAAATATGCATGGTCG+TGG - Chr6:14250090-14250109 MsG0680031096.01.T01:CDS 50.0%
CGTGGTCGTGGAAATATGCA+TGG - Chr6:14250084-14250103 MsG0680031096.01.T01:CDS 50.0%
GATGTGGGGGAAAAGGTCAT+TGG - Chr6:14250244-14250263 MsG0680031096.01.T01:CDS 50.0%
GGTCATGCCCACAATCCTAA+TGG - Chr6:14250117-14250136 MsG0680031096.01.T01:CDS 50.0%
!! CAACTATTTGACTTGGGCCG+TGG - Chr6:14249468-14249487 MsG0680031096.01.T01:CDS 50.0%
!!! AATCATTTTGGGAGCGCTCG+TGG - Chr6:14250030-14250049 MsG0680031096.01.T01:CDS 50.0%
GGAATGGAGCTACACCAGCA+GGG + Chr6:14249987-14250006 None:intergenic 55.0%
! CCCCATTTTCCCACCAGAAG+TGG - Chr6:14250151-14250170 MsG0680031096.01.T01:CDS 55.0%
AACCATGAAGCTCGCCCTGC+TGG - Chr6:14249970-14249989 MsG0680031096.01.T01:CDS 60.0%
GGGAATGGAGCTACACCAGC+AGG + Chr6:14249988-14250007 None:intergenic 60.0%
CACCAGCAGGGCGAGCTTCA+TGG + Chr6:14249975-14249994 None:intergenic 65.0%
!! TTTGGGAGCGCTCGTGGTCG+TGG - Chr6:14250036-14250055 MsG0680031096.01.T01:CDS 65.0%
AGCGCTCGTGGTCGTGGTCG+TGG - Chr6:14250042-14250061 MsG0680031096.01.T01:CDS 70.0%
CGTGGTCGTGGTCGTGGTCG+CGG - Chr6:14250048-14250067 MsG0680031096.01.T01:CDS 70.0%
CGCGGTCGTGGTCGTGGTCG+TGG - Chr6:14250072-14250091 MsG0680031096.01.T01:CDS 75.0%
CGTGGTCGTGGTCGCGGTCG+CGG - Chr6:14250054-14250073 MsG0680031096.01.T01:CDS 75.0%
! CGCGGTCGCGGTCGTGGTCG+TGG - Chr6:14250066-14250085 MsG0680031096.01.T01:CDS 80.0%
! CGTGGTCGCGGTCGCGGTCG+TGG - Chr6:14250060-14250079 MsG0680031096.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr6 gene 14249418 14250491 14249418 ID=MsG0680031096.01;Name=MsG0680031096.01
Chr6 mRNA 14249418 14250491 14249418 ID=MsG0680031096.01.T01;Parent=MsG0680031096.01;Name=MsG0680031096.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|357
Chr6 exon 14249418 14250491 14249418 ID=MsG0680031096.01.T01:exon:9795;Parent=MsG0680031096.01.T01
Chr6 CDS 14249418 14250491 14249418 ID=MsG0680031096.01.T01:cds;Parent=MsG0680031096.01.T01
Gene Sequence

>MsG0680031096.01.T01

ATGTCAAATCTCTCAAAACTTCACTTTGAGGCCCTTAACATTTCTGGAAACAACTATTTGACTTGGGCCGTGGATACTGAAATGTACTTAGCTGCTGAAGGAAATGGAGATGCCATAAAAGAAGGAAATAAGGCATCCGAGCAACAAAAAGCAAAAGCATTAATATTCCTTCGTCGCCATATAAATGAAGCACTTAAGAATGAATACCTCACTGTGAAAGATCCACTTGTGCTCTGGAATAAACTAAAAGAAAGATATGAGCACTTGAAAGCCATTATCCTCCCAAAAGCAAGATATGATTGGATGCACTTGCGCTTACAAGACTTTAAGTCTGTAACTGCCTATAATTCTGAAGTATACAAAATTACTTCTCAATTAGAATTGTGTGGTGAAAAAATAACAGATGCGGATCTGTTAGAGAAAACATTTTCAACCTTTCATGCATCCAATATGCTCTTGCAACAACAATACCGTGAAAGGGGATTTCAAAAATACTCTAATTTAATTTCTTGTCTCCTGGTTGCTGAACAGAACAATGAGCTTCTAATGAAAAACCATGAAGCTCGCCCTGCTGGTGTAGCTCCATTCCCTGAAGTGAATGCATCTCAACACAATCATTTTGGGAGCGCTCGTGGTCGTGGTCGTGGTCGCGGTCGCGGTCGTGGTCGTGGTCGTGGAAATATGCATGGTCGTGGTCGTGGTCATGCCCACAATCCTAATGGAAAATTCAGAACCCCATTTTCCCACCAGAAGTGGAAAAATAATGAAAAGAATGAAAATGAGAAGGGTGGACAAAGTAGCAAAACAAATGAAAGTATCTGTTATCGATGTGGGGGAAAAGGTCATTGGTCTCGTACATGTCGTACTCCAAAGCATTTGGTTGACCTTTATCAGCAATCACTAAAAAACAAAGGAAAAATGGTTGAAACTCATTATGCTTATAAAGATGGTGATGATGCTGATTATGATATTTATGGTGACATGAATACTACTCCTTTGGATATTGGTGATTTCTTTGAAAATCAAAATGAAAAAATTGATCACCTTGTTGAAGATGGAATCGTGAAGAAATAG

Protein sequence

>MsG0680031096.01.T01

MSNLSKLHFEALNISGNNYLTWAVDTEMYLAAEGNGDAIKEGNKASEQQKAKALIFLRRHINEALKNEYLTVKDPLVLWNKLKERYEHLKAIILPKARYDWMHLRLQDFKSVTAYNSEVYKITSQLELCGEKITDADLLEKTFSTFHASNMLLQQQYRERGFQKYSNLISCLLVAEQNNELLMKNHEARPAGVAPFPEVNASQHNHFGSARGRGRGRGRGRGRGRGNMHGRGRGHAHNPNGKFRTPFSHQKWKNNEKNENEKGGQSSKTNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLKNKGKMVETHYAYKDGDDADYDIYGDMNTTPLDIGDFFENQNEKIDHLVEDGIVKK*