Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013494.01.T01 | BBN68624.1 | 59.615 | 104 | 42 | 0 | 38 | 141 | 378 | 481 | 1.08E-30 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013494.01.T01 | P28002 | 45.122 | 82 | 45 | 0 | 28 | 109 | 254 | 335 | 6.11E-17 | 79.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013494.01.T01 | A0A5H2XND2 | 59.615 | 104 | 42 | 0 | 38 | 141 | 378 | 481 | 5.14e-31 | 126 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048909.01 | MsG0380013494.01 | 0.804075 | 2.571310e-49 | 1.313508e-46 |
| MsG0180001249.01 | MsG0380013494.01 | 0.808134 | 3.595590e-50 | 2.039488e-47 |
| MsG0180004504.01 | MsG0380013494.01 | 0.802124 | 6.511918e-49 | 3.164315e-46 |
| MsG0280011279.01 | MsG0380013494.01 | 0.815455 | 9.166351e-52 | 6.322904e-49 |
| MsG0380013493.01 | MsG0380013494.01 | 0.896150 | 4.859374e-76 | 5.192688e-72 |
| MsG0380013494.01 | MsG0480018108.01 | 0.805579 | 1.247163e-49 | 6.622005e-47 |
| MsG0380013494.01 | MsG0480022626.01 | 0.802823 | 4.673616e-49 | 2.312073e-46 |
| MsG0380013494.01 | MsG0480023502.01 | 0.807787 | 4.261030e-50 | 2.394989e-47 |
| MsG0380013494.01 | MsG0780039859.01 | 0.815422 | 9.320264e-52 | 6.423076e-49 |
| MsG0380013494.01 | MsG0780040437.01 | 0.812839 | 3.464833e-51 | 2.226202e-48 |
| MsG0380013494.01 | MsG0780041478.01 | 0.803549 | 3.306873e-49 | 1.666591e-46 |
| MsG0380013494.01 | MsG0880042006.01 | 0.838014 | 3.697912e-57 | 4.889929e-54 |
| MsG0380013494.01 | MsG0880042921.01 | 0.813412 | 2.593300e-51 | 1.692416e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013494.01.T01 | MTR_8g068690 | 76.712 | 73 | 17 | 0 | 38 | 110 | 277 | 349 | 2.14e-32 | 119 |
| MsG0380013494.01.T01 | MTR_0591s0010 | 69.863 | 73 | 22 | 0 | 38 | 110 | 278 | 350 | 2.10e-28 | 108 |
| MsG0380013494.01.T01 | MTR_8g068760 | 59.302 | 86 | 31 | 1 | 25 | 110 | 125 | 206 | 6.90e-27 | 101 |
| MsG0380013494.01.T01 | MTR_8g068570 | 70.149 | 67 | 20 | 0 | 38 | 104 | 251 | 317 | 2.16e-26 | 102 |
| MsG0380013494.01.T01 | MTR_8g068750 | 64.384 | 73 | 26 | 0 | 38 | 110 | 278 | 350 | 2.03e-25 | 100 |
| MsG0380013494.01.T01 | MTR_8g068580 | 71.875 | 64 | 18 | 0 | 47 | 110 | 43 | 106 | 1.99e-24 | 92.4 |
| MsG0380013494.01.T01 | MTR_6g078850 | 44.444 | 108 | 45 | 1 | 3 | 110 | 264 | 356 | 7.93e-23 | 94.0 |
| MsG0380013494.01.T01 | MTR_7g118320 | 58.904 | 73 | 30 | 0 | 38 | 110 | 263 | 335 | 2.38e-22 | 91.7 |
| MsG0380013494.01.T01 | MTR_8g068730 | 58.904 | 73 | 30 | 0 | 38 | 110 | 246 | 318 | 1.11e-21 | 89.7 |
| MsG0380013494.01.T01 | MTR_8g068870 | 44.444 | 108 | 45 | 1 | 3 | 110 | 244 | 336 | 5.59e-21 | 88.2 |
| MsG0380013494.01.T01 | MTR_8g024160 | 36.842 | 133 | 63 | 3 | 3 | 133 | 209 | 322 | 4.15e-19 | 82.4 |
| MsG0380013494.01.T01 | MTR_7g118300 | 47.674 | 86 | 38 | 1 | 30 | 108 | 268 | 353 | 5.32e-18 | 79.7 |
| MsG0380013494.01.T01 | MTR_3g092900 | 43.902 | 82 | 46 | 0 | 28 | 109 | 254 | 335 | 2.45e-17 | 77.8 |
| MsG0380013494.01.T01 | MTR_5g098170 | 41.463 | 82 | 48 | 0 | 28 | 109 | 254 | 335 | 3.36e-16 | 74.7 |
| MsG0380013494.01.T01 | MTR_1g036460 | 45.455 | 66 | 36 | 0 | 38 | 103 | 270 | 335 | 2.20e-11 | 61.2 |
| MsG0380013494.01.T01 | MTR_7g011990 | 39.394 | 66 | 40 | 0 | 38 | 103 | 268 | 333 | 2.23e-11 | 61.2 |
| MsG0380013494.01.T01 | MTR_1g036490 | 45.455 | 66 | 36 | 0 | 38 | 103 | 270 | 335 | 4.44e-11 | 60.5 |
| MsG0380013494.01.T01 | MTR_3g021440 | 43.284 | 67 | 38 | 0 | 38 | 104 | 272 | 338 | 4.62e-11 | 60.1 |
| MsG0380013494.01.T01 | MTR_3g021430 | 35.000 | 100 | 50 | 1 | 3 | 102 | 239 | 323 | 7.24e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013494.01.T01 | AT5G54160 | 37.805 | 82 | 51 | 0 | 28 | 109 | 252 | 333 | 3.26e-14 | 69.3 |
| MsG0380013494.01.T01 | AT1G77530 | 35.780 | 109 | 66 | 3 | 3 | 107 | 244 | 352 | 9.56e-13 | 65.1 |
| MsG0380013494.01.T01 | AT1G76790 | 43.662 | 71 | 39 | 1 | 28 | 97 | 255 | 325 | 1.29e-12 | 64.7 |
| MsG0380013494.01.T01 | AT1G21100 | 35.185 | 108 | 54 | 2 | 3 | 109 | 253 | 345 | 3.07e-12 | 63.5 |
| MsG0380013494.01.T01 | AT1G21120 | 35.185 | 108 | 54 | 2 | 3 | 109 | 253 | 345 | 3.35e-12 | 63.5 |
| MsG0380013494.01.T01 | AT1G51990 | 41.096 | 73 | 42 | 1 | 38 | 109 | 262 | 334 | 3.83e-12 | 63.5 |
| MsG0380013494.01.T01 | AT1G21110 | 42.466 | 73 | 41 | 1 | 38 | 109 | 273 | 345 | 4.26e-12 | 63.2 |
| MsG0380013494.01.T01 | AT1G51990 | 41.096 | 73 | 42 | 1 | 38 | 109 | 262 | 334 | 4.47e-12 | 63.2 |
| MsG0380013494.01.T01 | AT1G21120 | 39.759 | 83 | 49 | 1 | 28 | 109 | 311 | 393 | 5.26e-12 | 63.2 |
| MsG0380013494.01.T01 | AT1G21130 | 35.185 | 108 | 54 | 2 | 3 | 109 | 253 | 345 | 5.81e-12 | 62.8 |
| MsG0380013494.01.T01 | AT1G77520 | 34.862 | 109 | 67 | 3 | 3 | 107 | 244 | 352 | 1.70e-11 | 61.6 |
Find 30 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCAAGAATTTCTTGATT+TGG | 0.267890 | 3:+39366390 | MsG0380013494.01.T01:CDS |
| TTGCATCCGGTTGGCATAAC+TGG | 0.292352 | 3:+39365813 | MsG0380013494.01.T01:CDS |
| ACAATCACTTTCCCATCATT+AGG | 0.309630 | 3:-39366260 | None:intergenic |
| GCAACTAGAGCTAGATTTAG+AGG | 0.343248 | 3:+39366412 | MsG0380013494.01.T01:CDS |
| TGTTTGTAACAAATGGGTTA+TGG | 0.344306 | 3:+39366453 | MsG0380013494.01.T01:CDS |
| CCGGATGCAAAAGGGAATAT+TGG | 0.346907 | 3:-39365800 | None:intergenic |
| CAATCACTTTCCCATCATTA+GGG | 0.346994 | 3:-39366259 | None:intergenic |
| GAATATTGGCGAAACCCATT+TGG | 0.364748 | 3:-39365786 | None:intergenic |
| AATTCCACGTGTGTTGCTTA+TGG | 0.376088 | 3:+39366539 | MsG0380013494.01.T01:CDS |
| AATGTTGTGTTTGTAACAAA+TGG | 0.381937 | 3:+39366446 | MsG0380013494.01.T01:CDS |
| CAATTCCATCGTATCCCAAA+TGG | 0.412776 | 3:+39365771 | MsG0380013494.01.T01:CDS |
| TGTAGGGCGTACTTCATGAT+TGG | 0.415846 | 3:+39366189 | MsG0380013494.01.T01:intron |
| AATATTGGCGAAACCCATTT+GGG | 0.426977 | 3:-39365785 | None:intergenic |
| TCCTCACCAGTTATGCCAAC+CGG | 0.469473 | 3:-39365819 | None:intergenic |
| TTATGCCAACCGGATGCAAA+AGG | 0.481186 | 3:-39365809 | None:intergenic |
| ATGTTGTGTTTGTAACAAAT+GGG | 0.499795 | 3:+39366447 | MsG0380013494.01.T01:CDS |
| AATTCCATCGTATCCCAAAT+GGG | 0.501244 | 3:+39365772 | MsG0380013494.01.T01:CDS |
| TATGATGCTATCCCTAATGA+TGG | 0.537285 | 3:+39366248 | MsG0380013494.01.T01:CDS |
| TTATCATCGATCAATATGCT+TGG | 0.563978 | 3:+39365726 | None:intergenic |
| TGAGGACGAATCAAACCTAA+AGG | 0.568121 | 3:+39365836 | MsG0380013494.01.T01:CDS |
| GAATCATTCTCATGCGCAGT+GGG | 0.570620 | 3:-39366299 | None:intergenic |
| TATGCCAACCGGATGCAAAA+GGG | 0.573902 | 3:-39365808 | None:intergenic |
| TCTGATGATGACGCAAACCC+AGG | 0.576679 | 3:+39366352 | MsG0380013494.01.T01:CDS |
| CATCAGAACATCTAATTGAG+AGG | 0.589565 | 3:-39366336 | None:intergenic |
| TCCGGTTGGCATAACTGGTG+AGG | 0.630238 | 3:+39365818 | MsG0380013494.01.T01:CDS |
| AGAATCATTCTCATGCGCAG+TGG | 0.648421 | 3:-39366300 | None:intergenic |
| GAAACCCATTTGGGATACGA+TGG | 0.672669 | 3:-39365776 | None:intergenic |
| ATGATGCTATCCCTAATGAT+GGG | 0.676199 | 3:+39366249 | MsG0380013494.01.T01:CDS |
| TCAACCATAAGCAACACACG+TGG | 0.699881 | 3:-39366543 | None:intergenic |
| GATGATGACGCAAACCCAGG+AGG | 0.711651 | 3:+39366355 | MsG0380013494.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAATTAATATTAATAT+TGG | - | Chr3:39366131-39366150 | None:intergenic | 0.0% |
| !! | AAAAATGCTTAATTAAATAT+AGG | - | Chr3:39366091-39366110 | None:intergenic | 10.0% |
| !!! | TATGTTGACTAAATTTTGTA+GGG | + | Chr3:39366173-39366192 | MsG0380013494.01.T01:intron | 20.0% |
| ! | AATGTTGTGTTTGTAACAAA+TGG | + | Chr3:39366446-39366465 | MsG0380013494.01.T01:CDS | 25.0% |
| ! | AGATTCTAACAAAAGATTGT+TGG | + | Chr3:39365957-39365976 | MsG0380013494.01.T01:intron | 25.0% |
| ! | ATGTTGTGTTTGTAACAAAT+GGG | + | Chr3:39366447-39366466 | MsG0380013494.01.T01:CDS | 25.0% |
| ! | GTTTATATTCATCATCTTCT+TGG | + | Chr3:39365907-39365926 | MsG0380013494.01.T01:intron | 25.0% |
| ! | TAAGCAAGAATTTCTTGATT+TGG | + | Chr3:39366390-39366409 | MsG0380013494.01.T01:CDS | 25.0% |
| ! | TGGTCTTTATATATATGACT+CGG | + | Chr3:39366033-39366052 | MsG0380013494.01.T01:intron | 25.0% |
| !! | TATATATATGACTCGGATTT+CGG | + | Chr3:39366040-39366059 | MsG0380013494.01.T01:intron | 25.0% |
| !!! | GTATGTTGACTAAATTTTGT+AGG | + | Chr3:39366172-39366191 | MsG0380013494.01.T01:intron | 25.0% |
| ! | CTTTTGTTAGAATCTGTGTT+GGG | - | Chr3:39365952-39365971 | None:intergenic | 30.0% |
| ! | TCTTTTGTTAGAATCTGTGT+TGG | - | Chr3:39365953-39365972 | None:intergenic | 30.0% |
| ! | TTTTGTTAGAATCTGTGTTG+GGG | - | Chr3:39365951-39365970 | None:intergenic | 30.0% |
| !! | TGTTTGTAACAAATGGGTTA+TGG | + | Chr3:39366453-39366472 | MsG0380013494.01.T01:CDS | 30.0% |
| AATATTGGCGAAACCCATTT+GGG | - | Chr3:39365788-39365807 | None:intergenic | 35.0% | |
| AATTCAGCTTATGTAGCACT+TGG | - | Chr3:39366013-39366032 | None:intergenic | 35.0% | |
| AATTCCATCGTATCCCAAAT+GGG | + | Chr3:39365772-39365791 | MsG0380013494.01.T01:CDS | 35.0% | |
| ACAATCACTTTCCCATCATT+AGG | - | Chr3:39366263-39366282 | None:intergenic | 35.0% | |
| AGTGCTACATAAGCTGAATT+TGG | + | Chr3:39366013-39366032 | MsG0380013494.01.T01:intron | 35.0% | |
| ATAAATTCCAAAGCGTGTGT+GGG | - | Chr3:39365871-39365890 | None:intergenic | 35.0% | |
| ATGATGCTATCCCTAATGAT+GGG | + | Chr3:39366249-39366268 | MsG0380013494.01.T01:CDS | 35.0% | |
| CAATCACTTTCCCATCATTA+GGG | - | Chr3:39366262-39366281 | None:intergenic | 35.0% | |
| CATCAGAACATCTAATTGAG+AGG | - | Chr3:39366339-39366358 | None:intergenic | 35.0% | |
| TATGATGCTATCCCTAATGA+TGG | + | Chr3:39366248-39366267 | MsG0380013494.01.T01:CDS | 35.0% | |
| TTATGTAGCACTTGGAAAGT+GGG | - | Chr3:39366005-39366024 | None:intergenic | 35.0% | |
| !!! | TGGGAAAGTGATTGTTTTAG+AGG | + | Chr3:39366268-39366287 | MsG0380013494.01.T01:CDS | 35.0% |
| CAATTCCATCGTATCCCAAA+TGG | + | Chr3:39365771-39365790 | MsG0380013494.01.T01:CDS | 40.0% | |
| CATAAATTCCAAAGCGTGTG+TGG | - | Chr3:39365872-39365891 | None:intergenic | 40.0% | |
| CTTATGTAGCACTTGGAAAG+TGG | - | Chr3:39366006-39366025 | None:intergenic | 40.0% | |
| GAATATTGGCGAAACCCATT+TGG | - | Chr3:39365789-39365808 | None:intergenic | 40.0% | |
| GCAACTAGAGCTAGATTTAG+AGG | + | Chr3:39366412-39366431 | MsG0380013494.01.T01:CDS | 40.0% | |
| TGAGGACGAATCAAACCTAA+AGG | + | Chr3:39365836-39365855 | MsG0380013494.01.T01:CDS | 40.0% | |
| ! | CCAATATTCCCTTTTGCATC+CGG | + | Chr3:39365800-39365819 | MsG0380013494.01.T01:CDS | 40.0% |
| ! | TAGTTCTCTCTTTTCCTCCT+GGG | - | Chr3:39366372-39366391 | None:intergenic | 40.0% |
| !! | AATTCCACGTGTGTTGCTTA+TGG | + | Chr3:39366539-39366558 | MsG0380013494.01.T01:CDS | 40.0% |
| !! | TTAGTTCTCTCTTTTCCTCC+TGG | - | Chr3:39366373-39366392 | None:intergenic | 40.0% |
| AGAATCATTCTCATGCGCAG+TGG | - | Chr3:39366303-39366322 | None:intergenic | 45.0% | |
| CCGGATGCAAAAGGGAATAT+TGG | - | Chr3:39365803-39365822 | None:intergenic | 45.0% | |
| GAATCATTCTCATGCGCAGT+GGG | - | Chr3:39366302-39366321 | None:intergenic | 45.0% | |
| TATGCCAACCGGATGCAAAA+GGG | - | Chr3:39365811-39365830 | None:intergenic | 45.0% | |
| TCAACCATAAGCAACACACG+TGG | - | Chr3:39366546-39366565 | None:intergenic | 45.0% | |
| TGTAGGGCGTACTTCATGAT+TGG | + | Chr3:39366189-39366208 | MsG0380013494.01.T01:intron | 45.0% | |
| TGTGGGACACACTAACCTTT+AGG | - | Chr3:39365854-39365873 | None:intergenic | 45.0% | |
| TTATGCCAACCGGATGCAAA+AGG | - | Chr3:39365812-39365831 | None:intergenic | 45.0% | |
| ! | GAAACCCATTTGGGATACGA+TGG | - | Chr3:39365779-39365798 | None:intergenic | 45.0% |
| ! | TATTCCCTTTTGCATCCGGT+TGG | + | Chr3:39365804-39365823 | MsG0380013494.01.T01:CDS | 45.0% |
| GTAGCACTTGGAAAGTGGGA+AGG | - | Chr3:39366001-39366020 | None:intergenic | 50.0% | |
| TCCTCACCAGTTATGCCAAC+CGG | - | Chr3:39365822-39365841 | None:intergenic | 50.0% | |
| TTGCATCCGGTTGGCATAAC+TGG | + | Chr3:39365813-39365832 | MsG0380013494.01.T01:CDS | 50.0% | |
| ! | TCTGATGATGACGCAAACCC+AGG | + | Chr3:39366352-39366371 | MsG0380013494.01.T01:CDS | 50.0% |
| AGTGTGTCCCACACACGCTT+TGG | + | Chr3:39365861-39365880 | MsG0380013494.01.T01:intron | 55.0% | |
| GCACTTGGAAAGTGGGAAGG+TGG | - | Chr3:39365998-39366017 | None:intergenic | 55.0% | |
| TCCGGTTGGCATAACTGGTG+AGG | + | Chr3:39365818-39365837 | MsG0380013494.01.T01:CDS | 55.0% | |
| ! | GATGATGACGCAAACCCAGG+AGG | + | Chr3:39366355-39366374 | MsG0380013494.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 39365741 | 39366565 | 39365741 | ID=MsG0380013494.01;Name=MsG0380013494.01 |
| Chr3 | mRNA | 39365741 | 39366565 | 39365741 | ID=MsG0380013494.01.T01;Parent=MsG0380013494.01;Name=MsG0380013494.01.T01;_AED=0.68;_eAED=0.77;_QI=0|0|0|0.5|1|1|2|0|162 |
| Chr3 | exon | 39365741 | 39365857 | 39365741 | ID=MsG0380013494.01.T01:exon:22270;Parent=MsG0380013494.01.T01 |
| Chr3 | exon | 39366194 | 39366565 | 39366194 | ID=MsG0380013494.01.T01:exon:22271;Parent=MsG0380013494.01.T01 |
| Chr3 | CDS | 39365741 | 39365857 | 39365741 | ID=MsG0380013494.01.T01:cds;Parent=MsG0380013494.01.T01 |
| Chr3 | CDS | 39366194 | 39366565 | 39366194 | ID=MsG0380013494.01.T01:cds;Parent=MsG0380013494.01.T01 |
| Gene Sequence |
| Protein sequence |
