AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048698.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048698.01.T01 MTR_6g055020 95.425 153 7 0 1 153 79 231 4.32e-91 266
MsG0080048698.01.T01 MTR_7g117920 94.771 153 8 0 1 153 79 231 6.75e-90 264
MsG0080048698.01.T01 MTR_7g033765 95.425 153 7 0 1 153 79 231 1.34e-88 269
MsG0080048698.01.T01 MTR_7g021780 95.425 153 7 0 1 153 79 231 2.56e-88 269
MsG0080048698.01.T01 MTR_4g451275 94.771 153 8 0 1 153 79 231 8.07e-88 265
MsG0080048698.01.T01 MTR_3g035730 94.118 153 9 0 1 153 79 231 3.25e-87 263
MsG0080048698.01.T01 MTR_0002s0650 91.503 153 13 0 1 153 79 231 3.20e-85 261
MsG0080048698.01.T01 MTR_1g108765 73.103 145 39 0 1 145 138 282 1.81e-66 211
MsG0080048698.01.T01 MTR_1g108770 71.724 145 41 0 1 145 141 285 5.10e-65 207
MsG0080048698.01.T01 MTR_7g033755 71.242 153 11 1 1 153 40 159 4.16e-62 198
MsG0080048698.01.T01 MTR_4g010980 97.260 73 2 0 1 73 76 148 8.11e-45 144
MsG0080048698.01.T01 MTR_2g066870 52.419 124 59 0 22 145 29 152 1.73e-39 138
MsG0080048698.01.T01 MTR_0212s0040 34.615 104 68 0 7 110 84 187 4.13e-11 60.5
MsG0080048698.01.T01 MTR_0002s0550 28.571 147 102 2 5 149 79 224 4.82e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048698.01.T01 ATCG00480 91.503 153 13 0 1 153 79 231 2.22e-86 261
MsG0080048698.01.T01 AT5G08690 73.793 145 38 0 1 145 138 282 5.76e-66 210
MsG0080048698.01.T01 AT5G08670 73.793 145 38 0 1 145 138 282 6.27e-66 210
MsG0080048698.01.T01 AT5G08680 73.793 145 38 0 1 145 141 285 6.66e-66 210
MsG0080048698.01.T01 AT2G07698 26.974 152 103 1 6 149 353 504 1.26e-11 62.0
MsG0080048698.01.T01 ATCG00120 27.211 147 104 2 5 149 79 224 7.22e-11 59.7
MsG0080048698.01.T01 ATMG01190 26.974 152 103 1 6 149 83 234 9.82e-11 59.3

Find 34 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGCCTATTGATAATTT+AGG 0.258224 contig404end:+44934 MsG0080048698.01.T01:CDS
CATGGAGGTGTATCCGTATT+TGG 0.332634 contig404end:+45168 MsG0080048698.01.T01:CDS
AGGTAAAACAGTACTGATTA+TGG 0.333322 contig404end:+45119 MsG0080048698.01.T01:CDS
TGAGGGAAATGATCTTTATA+TGG 0.365509 contig404end:+45212 MsG0080048698.01.T01:CDS
CTCACCTACTCCGCCAAATA+CGG 0.404574 contig404end:-45181 None:intergenic
CCTAGGGTTGCTCCGCCGAC+TGG 0.429178 contig404end:-44892 None:intergenic
AATTTAGGTCCTGTAGATAC+TGG 0.436084 contig404end:+44949 MsG0080048698.01.T01:CDS
ACAGGACCTAAATTATCAAT+AGG 0.448331 contig404end:-44940 None:intergenic
TATATGGAAATGAAAGAATC+CGG 0.476570 contig404end:+45228 MsG0080048698.01.T01:CDS
TCCGCCGACTGGAACACTTA+GGG 0.488334 contig404end:-44881 None:intergenic
GGATACACCTCCATGAGCTT+TGG 0.495355 contig404end:-45160 None:intergenic
GCAGGCGCGGATCTATGAAT+AGG 0.503709 contig404end:-44982 None:intergenic
AATAACATTGCCAAAGCTCA+TGG 0.503738 contig404end:+45150 MsG0080048698.01.T01:CDS
CCAGTCGGCGGAGCAACCCT+AGG 0.517552 contig404end:+44892 MsG0080048698.01.T01:CDS
GTATCTAACTGTATAAAGGC+AGG 0.519520 contig404end:-45000 None:intergenic
CTCCGCCGACTGGAACACTT+AGG 0.525766 contig404end:-44882 None:intergenic
CCGCCGACTGGAACACTTAG+GGG 0.531894 contig404end:-44880 None:intergenic
CACGTTGAAAATTCGACCTA+GGG 0.533153 contig404end:-44908 None:intergenic
GATGTTGTGCCAGTATCTAC+AGG 0.533199 contig404end:-44958 None:intergenic
CCCCTAAGTGTTCCAGTCGG+CGG 0.555808 contig404end:+44880 MsG0080048698.01.T01:CDS
GCACGTTGAAAATTCGACCT+AGG 0.562793 contig404end:-44909 None:intergenic
AGATGGTCTAAAGAGAGGAA+TGG 0.562943 contig404end:+44837 MsG0080048698.01.T01:CDS
GTAGGTGAGCGTACTCGTGA+GGG 0.590163 contig404end:+45195 MsG0080048698.01.T01:CDS
GTATCCGTATTTGGCGGAGT+AGG 0.595672 contig404end:+45177 MsG0080048698.01.T01:CDS
AGTAGGTGAGCGTACTCGTG+AGG 0.604267 contig404end:+45194 MsG0080048698.01.T01:CDS
GGAATGGATGTGATTGACAC+GGG 0.632746 contig404end:+44853 MsG0080048698.01.T01:CDS
GGAGGTGTATCCGTATTTGG+CGG 0.636053 contig404end:+45171 MsG0080048698.01.T01:CDS
AGGAATGGATGTGATTGACA+CGG 0.636823 contig404end:+44852 MsG0080048698.01.T01:CDS
GCTACAGATGGTCTAAAGAG+AGG 0.640370 contig404end:+44832 MsG0080048698.01.T01:CDS
TTAGCTCCTTATCGCCGTGG+CGG 0.641710 contig404end:+45066 MsG0080048698.01.T01:CDS
GCTCCCCTAAGTGTTCCAGT+CGG 0.659074 contig404end:+44877 MsG0080048698.01.T01:CDS
GTAGCTATGAGTGCTACAGA+TGG 0.664604 contig404end:+44820 None:intergenic
TAACTGTATAAAGGCAGGCG+CGG 0.685033 contig404end:-44995 None:intergenic
AACATTGCCAAAGCTCATGG+AGG 0.715418 contig404end:+45153 MsG0080048698.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATTAATGAAAAAATATTG+CGG + contig404end:45253-45272 MsG0080048698.01.T01:CDS 15.0%
!! TTTAATTCCTGTTTCAAAAA+TGG - contig404end:45034-45053 None:intergenic 20.0%
!!! AAATTATCCATTTTTGAAAC+AGG + contig404end:45024-45043 MsG0080048698.01.T01:CDS 20.0%
!!! TATTTTTTCATTAATGACTC+CGG - contig404end:45250-45269 None:intergenic 20.0%
!!! TTTTGTATCTAACTGTATAA+AGG - contig404end:45007-45026 None:intergenic 20.0%
! TATATGGAAATGAAAGAATC+CGG + contig404end:45228-45247 MsG0080048698.01.T01:CDS 25.0%
ACAGGACCTAAATTATCAAT+AGG - contig404end:44943-44962 None:intergenic 30.0%
TGAGGGAAATGATCTTTATA+TGG + contig404end:45212-45231 MsG0080048698.01.T01:CDS 30.0%
!! AGGTAAAACAGTACTGATTA+TGG + contig404end:45119-45138 MsG0080048698.01.T01:CDS 30.0%
AATAACATTGCCAAAGCTCA+TGG + contig404end:45150-45169 MsG0080048698.01.T01:CDS 35.0%
AATTTAGGTCCTGTAGATAC+TGG + contig404end:44949-44968 MsG0080048698.01.T01:CDS 35.0%
GTATCTAACTGTATAAAGGC+AGG - contig404end:45003-45022 None:intergenic 35.0%
! GGAGAGCCTATTGATAATTT+AGG + contig404end:44934-44953 MsG0080048698.01.T01:CDS 35.0%
AGATGGTCTAAAGAGAGGAA+TGG + contig404end:44837-44856 MsG0080048698.01.T01:CDS 40.0%
AGGAATGGATGTGATTGACA+CGG + contig404end:44852-44871 MsG0080048698.01.T01:CDS 40.0%
CACGTTGAAAATTCGACCTA+GGG - contig404end:44911-44930 None:intergenic 40.0%
! GGAAAAATAGGACTTTTCGG+TGG + contig404end:45087-45106 MsG0080048698.01.T01:CDS 40.0%
! GGCGGAAAAATAGGACTTTT+CGG + contig404end:45084-45103 MsG0080048698.01.T01:CDS 40.0%
!! AAAGTCCTATTTTTCCGCCA+CGG - contig404end:45083-45102 None:intergenic 40.0%
AACATTGCCAAAGCTCATGG+AGG + contig404end:45153-45172 MsG0080048698.01.T01:CDS 45.0%
GATGTTGTGCCAGTATCTAC+AGG - contig404end:44961-44980 None:intergenic 45.0%
GCACGTTGAAAATTCGACCT+AGG - contig404end:44912-44931 None:intergenic 45.0%
GCTACAGATGGTCTAAAGAG+AGG + contig404end:44832-44851 MsG0080048698.01.T01:CDS 45.0%
GGAATGGATGTGATTGACAC+GGG + contig404end:44853-44872 MsG0080048698.01.T01:CDS 45.0%
TAACTGTATAAAGGCAGGCG+CGG - contig404end:44998-45017 None:intergenic 45.0%
! CATGGAGGTGTATCCGTATT+TGG + contig404end:45168-45187 MsG0080048698.01.T01:CDS 45.0%
! GGTCGAATTTTCAACGTGCT+TGG + contig404end:44913-44932 MsG0080048698.01.T01:CDS 45.0%
CTCACCTACTCCGCCAAATA+CGG - contig404end:45184-45203 None:intergenic 50.0%
GGATACACCTCCATGAGCTT+TGG - contig404end:45163-45182 None:intergenic 50.0%
GTATCCGTATTTGGCGGAGT+AGG + contig404end:45177-45196 MsG0080048698.01.T01:CDS 50.0%
! ATAGGACTTTTCGGTGGAGC+TGG + contig404end:45093-45112 MsG0080048698.01.T01:CDS 50.0%
! ATTTTTCCGCCACGGCGATA+AGG - contig404end:45075-45094 None:intergenic 50.0%
! GGAGGTGTATCCGTATTTGG+CGG + contig404end:45171-45190 MsG0080048698.01.T01:CDS 50.0%
! TAGGACTTTTCGGTGGAGCT+GGG + contig404end:45094-45113 MsG0080048698.01.T01:CDS 50.0%
! TATCGCCGTGGCGGAAAAAT+AGG + contig404end:45075-45094 MsG0080048698.01.T01:CDS 50.0%
!!! CTTTTAGCTCCTTATCGCCG+TGG + contig404end:45063-45082 MsG0080048698.01.T01:CDS 50.0%
AGTAGGTGAGCGTACTCGTG+AGG + contig404end:45194-45213 MsG0080048698.01.T01:CDS 55.0%
GCAGGCGCGGATCTATGAAT+AGG - contig404end:44985-45004 None:intergenic 55.0%
GCTCCCCTAAGTGTTCCAGT+CGG + contig404end:44877-44896 MsG0080048698.01.T01:CDS 55.0%
GTAGGTGAGCGTACTCGTGA+GGG + contig404end:45195-45214 MsG0080048698.01.T01:CDS 55.0%
TTAGCTCCTTATCGCCGTGG+CGG + contig404end:45066-45085 MsG0080048698.01.T01:CDS 55.0%
! AGGACTTTTCGGTGGAGCTG+GGG + contig404end:45095-45114 MsG0080048698.01.T01:CDS 55.0%
! TCCGCCGACTGGAACACTTA+GGG - contig404end:44884-44903 None:intergenic 55.0%
CCCCTAAGTGTTCCAGTCGG+CGG + contig404end:44880-44899 MsG0080048698.01.T01:CDS 60.0%
! CCGCCGACTGGAACACTTAG+GGG - contig404end:44883-44902 None:intergenic 60.0%
! CTCCGCCGACTGGAACACTT+AGG - contig404end:44885-44904 None:intergenic 60.0%
! CTTTTCGGTGGAGCTGGGGT+AGG + contig404end:45099-45118 MsG0080048698.01.T01:CDS 60.0%
CCAGTCGGCGGAGCAACCCT+AGG + contig404end:44892-44911 MsG0080048698.01.T01:CDS 70.0%
CCTAGGGTTGCTCCGCCGAC+TGG - contig404end:44895-44914 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
contig404end gene 44826 45287 44826 ID=MsG0080048698.01;Name=MsG0080048698.01
contig404end mRNA 44826 45287 44826 ID=MsG0080048698.01.T01;Parent=MsG0080048698.01;Name=MsG0080048698.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|153
contig404end exon 44826 45287 44826 ID=MsG0080048698.01.T01:exon:5315;Parent=MsG0080048698.01.T01
contig404end CDS 44826 45287 44826 ID=MsG0080048698.01.T01:cds;Parent=MsG0080048698.01.T01
Gene Sequence

>MsG0080048698.01.T01

ATGAGTGCTACAGATGGTCTAAAGAGAGGAATGGATGTGATTGACACGGGAGCTCCCCTAAGTGTTCCAGTCGGCGGAGCAACCCTAGGTCGAATTTTCAACGTGCTTGGAGAGCCTATTGATAATTTAGGTCCTGTAGATACTGGCACAACATCTCCTATTCATAGATCCGCGCCTGCCTTTATACAGTTAGATACAAAATTATCCATTTTTGAAACAGGAATTAAAGTAGTAGATCTTTTAGCTCCTTATCGCCGTGGCGGAAAAATAGGACTTTTCGGTGGAGCTGGGGTAGGTAAAACAGTACTGATTATGGAATTGATCAATAACATTGCCAAAGCTCATGGAGGTGTATCCGTATTTGGCGGAGTAGGTGAGCGTACTCGTGAGGGAAATGATCTTTATATGGAAATGAAAGAATCCGGAGTCATTAATGAAAAAATATTGCGGAATCAAAAGTAG

Protein sequence

>MsG0080048698.01.T01

MSATDGLKRGMDVIDTGAPLSVPVGGATLGRIFNVLGEPIDNLGPVDTGTTSPIHRSAPAFIQLDTKLSIFETGIKVVDLLAPYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEKILRNQK*