Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014014.01.T01 | AGV52558.1 | 85.294 | 68 | 10 | 0 | 1 | 68 | 11 | 78 | 2.43E-32 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014014.01.T01 | Q9BBN7 | 78.689 | 61 | 13 | 0 | 3 | 63 | 12 | 72 | 9.04E-27 | 95.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014014.01.T01 | W8CY92 | 85.294 | 68 | 10 | 0 | 1 | 68 | 11 | 78 | 1.16e-32 | 117 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013932.01 | MsG0380014014.01 | 0.812453 | 4.208509e-51 | 2.677075e-48 |
| MsG0380013950.01 | MsG0380014014.01 | 0.840785 | 7.055686e-58 | 1.015912e-54 |
| MsG0380013995.01 | MsG0380014014.01 | 0.803479 | 3.419102e-49 | 1.719995e-46 |
| MsG0380014012.01 | MsG0380014014.01 | 0.803670 | 3.120401e-49 | 1.577396e-46 |
| MsG0380014013.01 | MsG0380014014.01 | 0.813107 | 3.025423e-51 | 1.957860e-48 |
| MsG0380014014.01 | MsG0380014052.01 | 0.909707 | 4.229869e-82 | 8.335259e-78 |
| MsG0380014014.01 | MsG0380017006.01 | 0.850345 | 1.803034e-60 | 3.520869e-57 |
| MsG0380014014.01 | MsG0880045283.01 | 0.807186 | 5.715630e-50 | 3.162741e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014014.01.T01 | MTR_4g006090 | 88.889 | 63 | 7 | 0 | 1 | 63 | 11 | 73 | 9.83e-36 | 115 |
| MsG0380014014.01.T01 | MTR_6g042190 | 85.714 | 63 | 9 | 0 | 1 | 63 | 11 | 73 | 1.90e-34 | 112 |
| MsG0380014014.01.T01 | MTR_7g021690 | 91.803 | 61 | 5 | 0 | 3 | 63 | 1080 | 1140 | 1.51e-32 | 117 |
| MsG0380014014.01.T01 | MTR_0002s0210 | 62.295 | 61 | 23 | 0 | 3 | 63 | 15 | 75 | 2.87e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014014.01.T01 | ATCG01120 | 83.019 | 53 | 9 | 0 | 11 | 63 | 18 | 70 | 1.80e-25 | 90.1 |
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATTCAATTTCCATAATA+AGG | 0.215006 | 3:-48651992 | MsG0380014014.01.T01:CDS |
| CTTTGAGATAAATAGTCTTT+TGG | 0.244227 | 3:+48651937 | None:intergenic |
| AGGGGTTTACATATAATTCT+AGG | 0.296760 | 3:-48651916 | MsG0380014014.01.T01:CDS |
| ACTAAGACTTACTTCACATT+TGG | 0.336099 | 3:-48651968 | MsG0380014014.01.T01:CDS |
| TAAGTCTTAGTACCTTATTA+TGG | 0.341553 | 3:+48651980 | None:intergenic |
| AAAAGACTATTTATCTCAAA+GGG | 0.376816 | 3:-48651935 | MsG0380014014.01.T01:CDS |
| TACTTGAAATTCAACTGATC+CGG | 0.391504 | 3:+48652011 | None:intergenic |
| TACATATAATTCTAGGAAAA+CGG | 0.413634 | 3:-48651909 | MsG0380014014.01.T01:CDS |
| ACTTGAAATTCAACTGATCC+GGG | 0.489733 | 3:+48652012 | None:intergenic |
| CAAAAGACTATTTATCTCAA+AGG | 0.501338 | 3:-48651936 | MsG0380014014.01.T01:CDS |
| AAAGACTATTTATCTCAAAG+GGG | 0.651887 | 3:-48651934 | MsG0380014014.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTGTGAAATAAC+AGG | + | Chr3:48651879-48651898 | None:intergenic | 15.0% |
| !! | AAAAGACTATTTATCTCAAA+GGG | - | Chr3:48651991-48652010 | MsG0380014014.01.T01:CDS | 20.0% |
| !! | AGTATTCAATTTCCATAATA+AGG | - | Chr3:48651934-48651953 | MsG0380014014.01.T01:CDS | 20.0% |
| !!! | TACATATAATTCTAGGAAAA+CGG | - | Chr3:48652017-48652036 | MsG0380014014.01.T01:CDS | 20.0% |
| !!! | TTTGAGATAAATAGTCTTTT+GGG | + | Chr3:48651991-48652010 | None:intergenic | 20.0% |
| ! | AAAAAAAGAAGAAGAAAACC+CGG | - | Chr3:48651896-48651915 | MsG0380014014.01.T01:CDS | 25.0% |
| ! | AAAGACTATTTATCTCAAAG+GGG | - | Chr3:48651992-48652011 | MsG0380014014.01.T01:CDS | 25.0% |
| ! | CAAAAGACTATTTATCTCAA+AGG | - | Chr3:48651990-48652009 | MsG0380014014.01.T01:CDS | 25.0% |
| ! | TAAGTCTTAGTACCTTATTA+TGG | + | Chr3:48651949-48651968 | None:intergenic | 25.0% |
| !!! | CTTTGAGATAAATAGTCTTT+TGG | + | Chr3:48651992-48652011 | None:intergenic | 25.0% |
| ACTAAGACTTACTTCACATT+TGG | - | Chr3:48651958-48651977 | MsG0380014014.01.T01:CDS | 30.0% | |
| AGGGGTTTACATATAATTCT+AGG | - | Chr3:48652010-48652029 | MsG0380014014.01.T01:CDS | 30.0% | |
| TACTTGAAATTCAACTGATC+CGG | + | Chr3:48651918-48651937 | None:intergenic | 30.0% | |
| ACTTGAAATTCAACTGATCC+GGG | + | Chr3:48651917-48651936 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 48651855 | 48652093 | 48651855 | ID=MsG0380014014.01;Name=MsG0380014014.01 |
| Chr3 | mRNA | 48651855 | 48652061 | 48651855 | ID=MsG0380014014.01.T01;Parent=MsG0380014014.01;Name=MsG0380014014.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|68 |
| Chr3 | exon | 48651855 | 48652061 | 48651855 | ID=MsG0380014014.01.T01:exon:9428;Parent=MsG0380014014.01.T01 |
| Chr3 | CDS | 48651855 | 48652061 | 48651855 | ID=MsG0380014014.01.T01:cds;Parent=MsG0380014014.01.T01 |
| Chr3 | mRNA | 48651986 | 48652093 | 48651986 | ID=MsG0380014014.01.T02;Parent=MsG0380014014.01;Name=MsG0380014014.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|35 |
| Chr3 | exon | 48651986 | 48652093 | 48651986 | ID=MsG0380014014.01.T02:exon:9429;Parent=MsG0380014014.01.T02 |
| Chr3 | CDS | 48651986 | 48652093 | 48651986 | ID=MsG0380014014.01.T02:cds;Parent=MsG0380014014.01.T02 |
| Gene Sequence |
| Protein sequence |
