AlfalfaGEDB | MsG0380013950.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013950.01

KEGG: K02956
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00312

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013950.01.T01	YP_009141730.1	100	91	0	0	1	91	1	91	2.46E-57	181

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013950.01.T01	Q9BBN7	83.516	91	14	1	1	91	1	90	8.48E-46	144

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013950.01.T01	A0A4P8F435	100.000	91	0	0	1	91	1	91	1.18e-57	181

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002435.01	MsG0380013950.01	0.801104	1.054115e-48	4.992074e-46
MsG0380013918.01	MsG0380013950.01	0.821444	4.023622e-53	3.274040e-50
MsG0380013919.01	MsG0380013950.01	0.806296	8.812591e-50	4.765976e-47
MsG0380013920.01	MsG0380013950.01	0.818890	1.548055e-52	1.173394e-49
MsG0380013923.01	MsG0380013950.01	0.800513	1.391247e-48	6.490362e-46
MsG0380013929.01	MsG0380013950.01	0.814574	1.437635e-51	9.681993e-49
MsG0380013931.01	MsG0380013950.01	0.842051	3.274230e-58	4.904077e-55
MsG0380013932.01	MsG0380013950.01	0.908902	1.028354e-81	1.951058e-77
MsG0380013942.01	MsG0380013950.01	0.816254	6.080045e-52	4.286753e-49
MsG0380013944.01	MsG0380013950.01	0.811635	6.344370e-51	3.947402e-48
MsG0380013950.01	MsG0380013958.01	0.810716	1.003944e-50	6.096882e-48
MsG0380013950.01	MsG0380013964.01	0.845505	3.893839e-59	6.502877e-56
MsG0380013950.01	MsG0380013972.01	0.846428	2.185026e-59	3.760124e-56
MsG0380013950.01	MsG0380013980.01	0.838561	2.672645e-57	3.593171e-54
MsG0380013950.01	MsG0380013981.01	0.857860	1.223518e-62	3.062806e-59
MsG0380013950.01	MsG0380013983.01	0.804735	1.872606e-49	9.730403e-47
MsG0380013950.01	MsG0380013988.01	0.810284	1.244279e-50	7.469655e-48
MsG0380013950.01	MsG0380013995.01	0.894004	3.704414e-75	3.615532e-71
MsG0380013950.01	MsG0380014008.01	0.853459	2.356011e-61	5.094454e-58
MsG0380013950.01	MsG0380014009.01	0.823535	1.314787e-53	1.135141e-50
MsG0380013950.01	MsG0380014011.01	0.828141	1.060117e-54	1.044681e-51
MsG0380013950.01	MsG0380014014.01	0.840785	7.055686e-58	1.015912e-54
MsG0380013950.01	MsG0380014030.01	0.822791	1.960306e-53	1.657347e-50
MsG0380013950.01	MsG0380014035.01	0.816100	6.580345e-52	4.620057e-49
MsG0380013950.01	MsG0380014036.01	0.918136	2.259246e-86	6.771737e-82
MsG0380013950.01	MsG0380014039.01	0.801128	1.042026e-48	4.937797e-46
MsG0380013950.01	MsG0380014040.01	0.821807	3.317866e-53	2.727591e-50
MsG0380013950.01	MsG0380014041.01	0.830761	2.448063e-55	2.603861e-52
MsG0380013950.01	MsG0380014044.01	0.849749	2.647969e-60	5.070865e-57
MsG0380013950.01	MsG0380014051.01	0.855286	6.985894e-62	1.604759e-58
MsG0380013950.01	MsG0380014052.01	0.853247	2.711416e-61	5.821220e-58
MsG0380013950.01	MsG0380014055.01	0.822877	1.872156e-53	1.586698e-50
MsG0380013950.01	MsG0380017006.01	0.951783	9.839756e-110	2.308260e-104
MsG0380013950.01	MsG0380017007.01	0.822378	2.446283e-53	2.044180e-50
MsG0380013950.01	MsG0480022300.01	0.812592	3.922478e-51	2.504175e-48
MsG0380013950.01	MsG0780040316.01	0.816918	4.317083e-52	3.099414e-49
MsG0380013950.01	MsG0880044440.01	0.813954	1.970225e-51	1.304744e-48
MsG0380013950.01	MsG0880045283.01	0.803949	2.730305e-49	1.390279e-46
MsG0280007041.01	MsG0380013950.01	0.814384	1.583860e-51	1.061241e-48
MsG0280007042.01	MsG0380013950.01	0.847872	8.782637e-60	1.582777e-56

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013950.01.T01	MTR_4g006090	98.901	91	1	1	91	1	91	6.02e-61	180
MsG0380013950.01.T01	MTR_6g042190	95.604	91	4	1	91	1	91	9.20e-59	175
MsG0380013950.01.T01	MTR_7g021690	97.468	79	2	13	91	1080	1158	2.14e-46	158
MsG0380013950.01.T01	MTR_0002s0210	68.000	75	24	13	87	15	89	3.76e-21	82.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013950.01.T01	ATCG01120	70.330	91	24	1	1	91	1	88	1.38e-37	121

Find 14 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTATTCAATTTCCATAATA+AGG	0.215006	3:+48497783	MsG0380013950.01.T01:CDS
TAATAAATCAGTTGGGTATT+CGG	0.234341	3:+48497941	MsG0380013950.01.T01:CDS
CTTTGAGATAAATAGTCTTT+TGG	0.244227	3:-48497838	None:intergenic
AGGGGTTTACATATAATTCT+AGG	0.296760	3:+48497859	MsG0380013950.01.T01:CDS
AATAAATCAGTTGGGTATTC+GGG	0.307511	3:+48497942	MsG0380013950.01.T01:CDS
ACTAAGACTTACTTCACATT+TGG	0.336099	3:+48497807	MsG0380013950.01.T01:CDS
TAAGTCTTAGTACCTTATTA+TGG	0.341553	3:-48497795	None:intergenic
AAAAGACTATTTATCTCAAA+GGG	0.376816	3:+48497840	MsG0380013950.01.T01:CDS
TACTTGAAATTCAACTGATC+CGG	0.396257	3:-48497764	None:intergenic
TACATATAATTCTAGGAAAA+CGG	0.413634	3:+48497866	MsG0380013950.01.T01:CDS
ACTTGAAATTCAACTGATCC+GGG	0.468230	3:-48497763	None:intergenic
CAAAAGACTATTTATCTCAA+AGG	0.501338	3:+48497839	MsG0380013950.01.T01:CDS
CTTGAAATTCAACTGATCCG+GGG	0.649180	3:-48497762	None:intergenic
AAAGACTATTTATCTCAAAG+GGG	0.651887	3:+48497841	MsG0380013950.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAATTAATAAATCAGTT+GGG	+	Chr3:48497934-48497953	MsG0380013950.01.T01:CDS	10.0%
!!	TAAAAAATTAATAAATCAGT+TGG	+	Chr3:48497933-48497952	MsG0380013950.01.T01:CDS	10.0%
!!!	TTTTTTTTTTGTGAAATAAC+AGG	-	Chr3:48497730-48497749	None:intergenic	15.0%
!!	AAAAGACTATTTATCTCAAA+GGG	+	Chr3:48497840-48497859	MsG0380013950.01.T01:CDS	20.0%
!!	AGTATTCAATTTCCATAATA+AGG	+	Chr3:48497783-48497802	MsG0380013950.01.T01:CDS	20.0%
!!!	TACATATAATTCTAGGAAAA+CGG	+	Chr3:48497866-48497885	MsG0380013950.01.T01:CDS	20.0%
!!!	TTTGAGATAAATAGTCTTTT+GGG	-	Chr3:48497840-48497859	None:intergenic	20.0%
!	AAAGACTATTTATCTCAAAG+GGG	+	Chr3:48497841-48497860	MsG0380013950.01.T01:CDS	25.0%
!	CAAAAGACTATTTATCTCAA+AGG	+	Chr3:48497839-48497858	MsG0380013950.01.T01:CDS	25.0%
!	TAAGTCTTAGTACCTTATTA+TGG	-	Chr3:48497798-48497817	None:intergenic	25.0%
!	TAATAAATCAGTTGGGTATT+CGG	+	Chr3:48497941-48497960	MsG0380013950.01.T01:CDS	25.0%
!!!	CTTTGAGATAAATAGTCTTT+TGG	-	Chr3:48497841-48497860	None:intergenic	25.0%
	AAAAAAGAAGAAGAAAACCC+CGG	+	Chr3:48497745-48497764	MsG0380013950.01.T01:CDS	30.0%
	AATAAATCAGTTGGGTATTC+GGG	+	Chr3:48497942-48497961	MsG0380013950.01.T01:CDS	30.0%
	ACTAAGACTTACTTCACATT+TGG	+	Chr3:48497807-48497826	MsG0380013950.01.T01:CDS	30.0%
	AGGGGTTTACATATAATTCT+AGG	+	Chr3:48497859-48497878	MsG0380013950.01.T01:CDS	30.0%
	TACTTGAAATTCAACTGATC+CGG	-	Chr3:48497767-48497786	None:intergenic	30.0%
	ACTTGAAATTCAACTGATCC+GGG	-	Chr3:48497766-48497785	None:intergenic	35.0%
	CTTGAAATTCAACTGATCCG+GGG	-	Chr3:48497765-48497784	None:intergenic	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48497706	48497981	48497706	ID=MsG0380013950.01;Name=MsG0380013950.01
Chr3	mRNA	48497706	48497981	48497706	ID=MsG0380013950.01.T01;Parent=MsG0380013950.01;Name=MsG0380013950.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|91
Chr3	exon	48497706	48497981	48497706	ID=MsG0380013950.01.T01:exon:16748;Parent=MsG0380013950.01.T01
Chr3	CDS	48497706	48497981	48497706	ID=MsG0380013950.01.T01:cds;Parent=MsG0380013950.01.T01

Gene Sequence	>MsG0380013950.01.T01 ATGATAAAAAATTCATTTATACCTGTTATTTCACAAAAAAAAAAAGAAGAAGAAAACCCCGGATCAGTTGAATTTCAAGTATTCAATTTCCATAATAAGGTACTAAGACTTACTTCACATTTGGAATTACACCCAAAAGACTATTTATCTCAAAGGGGTTTACATATAATTCTAGGAAAACGGCAACGACTACTGTCTTATTTGTCAAAGAAAAATAAAATACGTTATAAAAAATTAATAAATCAGTTGGGTATTCGGGATTCACAAATTCGTTAA
Protein sequence	>MsG0380013950.01.T01 MIKNSFIPVISQKKKEEENPGSVEFQVFNFHNKVLRLTSHLELHPKDYLSQRGLHIILGKRQRLLSYLSKKNKIRYKKLINQLGIRDSQIR*