AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014012.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014012.01.T01 MTR_4g050880 98.361 61 1 0 1 61 1 61 1.04e-36 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014012.01.T01 ATCG00070 78.689 61 13 0 1 61 1 61 1.70e-28 96.7

Find 2 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTTAATATCTTTAGCT+TGG 0.440922 3:-48647394 MsG0380014012.01.T01:CDS
ACTGGCATAAAATCTACAAT+TGG 0.453871 3:+48647291 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAACTACTTGAATAA+AGG + Chr3:48647277-48647296 None:intergenic 20.0%
!! GAAAAAACTACTTGAATAAA+GGG + Chr3:48647276-48647295 None:intergenic 20.0%
!!! TTATTCAAGTAGTTTTTTCT+TGG - Chr3:48647277-48647296 MsG0380014012.01.T01:CDS 20.0%
!!! TTTTTCTCTTAGCTTTTGTT+TGG - Chr3:48647369-48647388 MsG0380014012.01.T01:CDS 25.0%
ACTGGCATAAAATCTACAAT+TGG + Chr3:48647335-48647354 None:intergenic 30.0%
AGCTAAGAGAAAAAAGAGTA+AGG + Chr3:48647363-48647382 None:intergenic 30.0%
GCTAAGAGAAAAAAGAGTAA+GGG + Chr3:48647362-48647381 None:intergenic 30.0%
! AATTGGATTCAAAAAAGCGT+AGG + Chr3:48647318-48647337 None:intergenic 30.0%
!! AAAAAGAGTAAGGGTATTAC+TGG + Chr3:48647353-48647372 None:intergenic 30.0%
! TTCAAAAAAGCGTAGGCTTC+AGG + Chr3:48647311-48647330 None:intergenic 40.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48647230 48647415 48647230 ID=MsG0380014012.01;Name=MsG0380014012.01
Chr3 mRNA 48647230 48647415 48647230 ID=MsG0380014012.01.T01;Parent=MsG0380014012.01;Name=MsG0380014012.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|61
Chr3 exon 48647230 48647415 48647230 ID=MsG0380014012.01.T01:exon:4682;Parent=MsG0380014012.01.T01
Chr3 CDS 48647230 48647415 48647230 ID=MsG0380014012.01.T01:cds;Parent=MsG0380014012.01.T01
Gene Sequence

>MsG0380014012.01.T01

ATGCTTAATATCTTTAGCTTGGTCTGTATTTGTATTGACTCTGCCCTTTATTCAAGTAGTTTTTTCTTGGCAAAATTACCTGAAGCCTACGCTTTTTTGAATCCAATTGTAGATTTTATGCCAGTAATACCCTTACTCTTTTTTCTCTTAGCTTTTGTTTGGCAAGCTGCTGTAAGTTTTCGATAA

Protein sequence

>MsG0380014012.01.T01

MLNIFSLVCICIDSALYSSSFFLAKLPEAYAFLNPIVDFMPVIPLLFFLLAFVWQAAVSFR*