Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015640.01.T01 | XP_013460851.1 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 1.43E-111 | 323 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015640.01.T01 | Q9SGZ8 | 49.375 | 160 | 63 | 7 | 1 | 151 | 1 | 151 | 1.57E-40 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015640.01.T01 | G8A369 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 6.82e-112 | 323 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000308.01 | MsG0380015640.01 | 0.829511 | 4.941298e-55 | 5.067897e-52 |
| MsG0180004277.01 | MsG0380015640.01 | 0.814661 | 1.375260e-51 | 9.283270e-49 |
| MsG0180005625.01 | MsG0380015640.01 | 0.800149 | 1.649966e-48 | 7.626499e-46 |
| MsG0380012353.01 | MsG0380015640.01 | 0.809789 | 1.590653e-50 | 9.426915e-48 |
| MsG0380012356.01 | MsG0380015640.01 | 0.800578 | 1.349433e-48 | 6.305751e-46 |
| MsG0380012357.01 | MsG0380015640.01 | 0.837541 | 4.890188e-57 | 6.372491e-54 |
| MsG0380012960.01 | MsG0380015640.01 | 0.815861 | 7.443237e-52 | 5.191318e-49 |
| MsG0380015049.01 | MsG0380015640.01 | 0.803473 | 3.428108e-49 | 1.724244e-46 |
| MsG0380015486.01 | MsG0380015640.01 | 0.807758 | 4.323150e-50 | 2.428025e-47 |
| MsG0380015640.01 | MsG0480020433.01 | 0.804087 | 2.555773e-49 | 1.305951e-46 |
| MsG0380015640.01 | MsG0480023998.01 | 0.803438 | 3.486505e-49 | 1.752025e-46 |
| MsG0380015640.01 | MsG0580024975.01 | 0.807013 | 6.219454e-50 | 3.426022e-47 |
| MsG0380015640.01 | MsG0580025678.01 | 0.802487 | 5.481351e-49 | 2.688313e-46 |
| MsG0380015640.01 | MsG0780037679.01 | 0.816157 | 6.391932e-52 | 4.494605e-49 |
| MsG0380015640.01 | MsG0780039323.01 | 0.820779 | 5.726496e-53 | 4.574142e-50 |
| MsG0380015640.01 | MsG0780039338.01 | 0.819204 | 1.313250e-52 | 1.004010e-49 |
| MsG0380015640.01 | MsG0780039934.01 | 0.810920 | 9.066334e-51 | 5.536282e-48 |
| MsG0380015640.01 | MsG0780040681.01 | 0.809700 | 1.661929e-50 | 9.826131e-48 |
| MsG0380015640.01 | MsG0780041468.01 | 0.809654 | 1.700062e-50 | 1.003959e-47 |
| MsG0380015640.01 | MsG0880046296.01 | 0.888833 | 4.155394e-73 | 3.270528e-69 |
| MsG0380015640.01 | MsG0880046573.01 | 0.803952 | 2.727181e-49 | 1.388760e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015640.01.T01 | MTR_3g073960 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 1.73e-115 | 323 |
| MsG0380015640.01.T01 | MTR_3g087700 | 37.791 | 172 | 73 | 7 | 1 | 138 | 1 | 172 | 5.95e-25 | 95.1 |
| MsG0380015640.01.T01 | MTR_2g436020 | 45.588 | 68 | 35 | 1 | 65 | 132 | 41 | 106 | 1.78e-15 | 69.3 |
| MsG0380015640.01.T01 | MTR_2g436250 | 45.588 | 68 | 35 | 1 | 65 | 132 | 41 | 106 | 1.78e-15 | 69.3 |
| MsG0380015640.01.T01 | MTR_8g008840 | 45.312 | 64 | 35 | 0 | 81 | 144 | 29 | 92 | 4.25e-15 | 67.8 |
| MsG0380015640.01.T01 | MTR_5g013770 | 59.184 | 49 | 19 | 1 | 86 | 134 | 81 | 128 | 8.01e-15 | 68.2 |
| MsG0380015640.01.T01 | MTR_4g120160 | 58.140 | 43 | 18 | 0 | 90 | 132 | 62 | 104 | 2.52e-14 | 65.5 |
| MsG0380015640.01.T01 | MTR_2g042970 | 59.574 | 47 | 18 | 1 | 89 | 135 | 185 | 230 | 2.74e-13 | 65.5 |
| MsG0380015640.01.T01 | MTR_4g099050 | 53.061 | 49 | 23 | 0 | 86 | 134 | 69 | 117 | 9.45e-13 | 62.4 |
| MsG0380015640.01.T01 | MTR_4g124930 | 44.828 | 58 | 31 | 1 | 90 | 147 | 178 | 234 | 6.78e-12 | 61.6 |
| MsG0380015640.01.T01 | MTR_4g124930 | 54.348 | 46 | 20 | 1 | 90 | 135 | 178 | 222 | 2.40e-11 | 60.1 |
| MsG0380015640.01.T01 | MTR_8g022950 | 52.273 | 44 | 21 | 0 | 90 | 133 | 39 | 82 | 2.54e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015640.01.T01 | AT1G78020 | 49.375 | 160 | 63 | 7 | 1 | 151 | 1 | 151 | 1.60e-41 | 136 |
| MsG0380015640.01.T01 | AT1G22160 | 50.667 | 150 | 60 | 5 | 1 | 149 | 1 | 137 | 8.76e-41 | 134 |
| MsG0380015640.01.T01 | AT4G39795 | 38.298 | 141 | 64 | 2 | 1 | 138 | 1 | 121 | 6.99e-25 | 93.2 |
| MsG0380015640.01.T01 | AT5G65040 | 37.324 | 142 | 56 | 4 | 1 | 142 | 1 | 109 | 1.25e-21 | 84.3 |
| MsG0380015640.01.T01 | AT5G49120 | 50.704 | 71 | 27 | 2 | 76 | 142 | 58 | 124 | 2.12e-17 | 74.7 |
| MsG0380015640.01.T01 | AT4G17670 | 60.417 | 48 | 19 | 0 | 87 | 134 | 73 | 120 | 8.84e-16 | 70.5 |
| MsG0380015640.01.T01 | AT5G47060 | 63.830 | 47 | 17 | 0 | 88 | 134 | 95 | 141 | 2.09e-15 | 70.1 |
| MsG0380015640.01.T01 | AT5G47060 | 63.830 | 47 | 17 | 0 | 88 | 134 | 95 | 141 | 2.09e-15 | 70.1 |
| MsG0380015640.01.T01 | AT1G19200 | 45.679 | 81 | 37 | 3 | 73 | 153 | 149 | 222 | 1.70e-13 | 65.9 |
| MsG0380015640.01.T01 | AT2G44670 | 53.191 | 47 | 22 | 0 | 88 | 134 | 14 | 60 | 1.87e-13 | 62.8 |
| MsG0380015640.01.T01 | AT1G19200 | 45.679 | 81 | 37 | 3 | 73 | 153 | 136 | 209 | 1.89e-13 | 65.5 |
| MsG0380015640.01.T01 | AT1G74940 | 47.143 | 70 | 31 | 3 | 89 | 153 | 149 | 217 | 4.89e-13 | 64.7 |
| MsG0380015640.01.T01 | AT5G20700 | 50.000 | 46 | 22 | 1 | 90 | 135 | 182 | 226 | 1.09e-11 | 61.2 |
| MsG0380015640.01.T01 | AT1G53885 | 50.000 | 44 | 22 | 0 | 90 | 133 | 42 | 85 | 7.62e-11 | 57.0 |
| MsG0380015640.01.T01 | AT1G53903 | 50.000 | 44 | 22 | 0 | 90 | 133 | 42 | 85 | 7.62e-11 | 57.0 |
Find 32 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGGAGAAGTTGGTGGATA+TGG | 0.255956 | 3:+72931844 | None:intergenic |
| GAATTTCTTCTAAGGTTTCT+AGG | 0.264307 | 3:+72931778 | None:intergenic |
| TATCAGCTGAATTTCTTCTA+AGG | 0.311688 | 3:+72931770 | None:intergenic |
| AAGCAAGAGCGTAAGAAATC+AGG | 0.327281 | 3:+72931736 | None:intergenic |
| AGAAGTAAGAAGATGATGTT+AGG | 0.329332 | 3:-72932009 | None:intergenic |
| TGATCTGAACCGTTGATTCC+AGG | 0.372333 | 3:+72931826 | None:intergenic |
| CTTTCTCTGTAAACGCCGTT+TGG | 0.383139 | 3:-72931716 | MsG0380015640.01.T01:CDS |
| TGTAAACGCCGTTTGGTACC+TGG | 0.425032 | 3:-72931709 | MsG0380015640.01.T01:CDS |
| TTTCATATGCTACAAATTTG+TGG | 0.444162 | 3:+72931251 | None:intergenic |
| CCATATCCACCAACTTCTCC+TGG | 0.454006 | 3:-72931844 | MsG0380015640.01.T01:CDS |
| AAACAAGTTGTAGTTGCAGC+TGG | 0.457073 | 3:-72931288 | MsG0380015640.01.T01:CDS |
| GAAGTAAGAAGATGATGTTA+GGG | 0.462099 | 3:-72932008 | None:intergenic |
| CAGGTCCATGACGGTTGAAA+AGG | 0.471195 | 3:+72931884 | None:intergenic |
| AACCGTCATGGACCTGGTGT+TGG | 0.498303 | 3:-72931877 | MsG0380015640.01.T01:CDS |
| AGCAAGAGCGTAAGAAATCA+GGG | 0.500012 | 3:+72931737 | None:intergenic |
| TCAACGGTTCAGATCAGAGC+AGG | 0.509698 | 3:-72931819 | MsG0380015640.01.T01:CDS |
| CCGTTGATTCCAGGAGAAGT+TGG | 0.513302 | 3:+72931835 | None:intergenic |
| GGACCTGGTGTTGGTCCGTA+TGG | 0.515066 | 3:-72931868 | MsG0380015640.01.T01:CDS |
| TGTTACTGTGTTCAGATCGA+AGG | 0.548881 | 3:+72931936 | None:intergenic |
| TTGATTCCAGGAGAAGTTGG+TGG | 0.560507 | 3:+72931838 | None:intergenic |
| AACAAGTTGTAGTTGCAGCT+GGG | 0.574251 | 3:-72931287 | MsG0380015640.01.T01:CDS |
| TCAGAGCAGGGTTATGTCCA+TGG | 0.575433 | 3:-72931806 | MsG0380015640.01.T01:CDS |
| GTTGGTGGATATGGACCATA+CGG | 0.587703 | 3:+72931853 | None:intergenic |
| AGTTGCAGCTGGGTCTAAAG+TGG | 0.592297 | 3:-72931277 | MsG0380015640.01.T01:CDS |
| GGACCATACGGACCAACACC+AGG | 0.604586 | 3:+72931865 | None:intergenic |
| CCAACTTCTCCTGGAATCAA+CGG | 0.605664 | 3:-72931835 | MsG0380015640.01.T01:CDS |
| TGTCACGACCAGGTACCAAA+CGG | 0.608597 | 3:+72931701 | None:intergenic |
| AAGGTTTCTAGGTGATACCA+TGG | 0.614252 | 3:+72931789 | None:intergenic |
| GAAGAAAGATAAGTGTTCTG+TGG | 0.638421 | 3:-72931319 | MsG0380015640.01.T01:CDS |
| GACCAACACCAGGTCCATGA+CGG | 0.641553 | 3:+72931875 | None:intergenic |
| TACATGTAGATGTCACGACC+AGG | 0.649454 | 3:+72931691 | None:intergenic |
| CAACGGTTCAGATCAGAGCA+GGG | 0.716813 | 3:-72931818 | MsG0380015640.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTATTATTAATTTTGTG+TGG | - | Chr3:72931803-72931822 | MsG0380015640.01.T01:CDS | 10.0% |
| ! | GTGAAAAATTAAATTTGCAG+AGG | - | Chr3:72931861-72931880 | MsG0380015640.01.T01:CDS | 25.0% |
| !! | AATTTTGTGTGGATATAGAA+GGG | - | Chr3:72931814-72931833 | MsG0380015640.01.T01:CDS | 25.0% |
| !! | TAATTTTGTGTGGATATAGA+AGG | - | Chr3:72931813-72931832 | MsG0380015640.01.T01:CDS | 25.0% |
| !!! | TTTTTTGCATCTAAAAGTCT+TGG | - | Chr3:72931629-72931648 | MsG0380015640.01.T01:intron | 25.0% |
| TATCAGCTGAATTTCTTCTA+AGG | + | Chr3:72931490-72931509 | None:intergenic | 30.0% | |
| ! | AAAAGGTTGTTGTTGTTGTT+AGG | + | Chr3:72931359-72931378 | None:intergenic | 30.0% |
| !! | GAATTTCTTCTAAGGTTTCT+AGG | + | Chr3:72931482-72931501 | None:intergenic | 30.0% |
| !!! | ATGCTTGTTGTTCTTTTCAT+TGG | + | Chr3:72931290-72931309 | None:intergenic | 30.0% |
| CAGAGACATTTGATACGTTA+GGG | + | Chr3:72931716-72931735 | None:intergenic | 35.0% | |
| CTTGTGAACACATCTCATAT+CGG | + | Chr3:72931748-72931767 | None:intergenic | 35.0% | |
| GAAGAAAGATAAGTGTTCTG+TGG | - | Chr3:72931938-72931957 | MsG0380015640.01.T01:CDS | 35.0% | |
| ! | ATAGAAGGGTGTGTGATAAT+TGG | - | Chr3:72931828-72931847 | MsG0380015640.01.T01:CDS | 35.0% |
| !!! | CTTGTTGTTCTTTTCATTGG+AGG | + | Chr3:72931287-72931306 | None:intergenic | 35.0% |
| AAACAAGTTGTAGTTGCAGC+TGG | - | Chr3:72931969-72931988 | MsG0380015640.01.T01:CDS | 40.0% | |
| AACAAGTTGTAGTTGCAGCT+GGG | - | Chr3:72931970-72931989 | MsG0380015640.01.T01:CDS | 40.0% | |
| ACTAAGTTTGATACCAGACG+CGG | - | Chr3:72931667-72931686 | MsG0380015640.01.T01:intron | 40.0% | |
| GCAGAGACATTTGATACGTT+AGG | + | Chr3:72931717-72931736 | None:intergenic | 40.0% | |
| TGTTACTGTGTTCAGATCGA+AGG | + | Chr3:72931324-72931343 | None:intergenic | 40.0% | |
| ! | AACAACCTTTTCAACCGTCA+TGG | - | Chr3:72931368-72931387 | MsG0380015640.01.T01:CDS | 40.0% |
| ! | AAGCAAGAGCGTAAGAAATC+AGG | + | Chr3:72931524-72931543 | None:intergenic | 40.0% |
| ! | AAGGTTTCTAGGTGATACCA+TGG | + | Chr3:72931471-72931490 | None:intergenic | 40.0% |
| ! | AGCAAGAGCGTAAGAAATCA+GGG | + | Chr3:72931523-72931542 | None:intergenic | 40.0% |
| CCAACTTCTCCTGGAATCAA+CGG | - | Chr3:72931422-72931441 | MsG0380015640.01.T01:intron | 45.0% | |
| CTTTCTCTGTAAACGCCGTT+TGG | - | Chr3:72931541-72931560 | MsG0380015640.01.T01:intron | 45.0% | |
| TACATGTAGATGTCACGACC+AGG | + | Chr3:72931569-72931588 | None:intergenic | 45.0% | |
| TTGATTCCAGGAGAAGTTGG+TGG | + | Chr3:72931422-72931441 | None:intergenic | 45.0% | |
| ! | GTTCTTTTCATTGGAGGACG+AGG | + | Chr3:72931281-72931300 | None:intergenic | 45.0% |
| ! | GTTGGTGGATATGGACCATA+CGG | + | Chr3:72931407-72931426 | None:intergenic | 45.0% |
| !! | TGATCTGAACCGTTGATTCC+AGG | + | Chr3:72931434-72931453 | None:intergenic | 45.0% |
| AGTTGCAGCTGGGTCTAAAG+TGG | - | Chr3:72931980-72931999 | MsG0380015640.01.T01:CDS | 50.0% | |
| CAACGGTTCAGATCAGAGCA+GGG | - | Chr3:72931439-72931458 | MsG0380015640.01.T01:intron | 50.0% | |
| CAGGTCCATGACGGTTGAAA+AGG | + | Chr3:72931376-72931395 | None:intergenic | 50.0% | |
| CCATATCCACCAACTTCTCC+TGG | - | Chr3:72931413-72931432 | MsG0380015640.01.T01:intron | 50.0% | |
| TCAACGGTTCAGATCAGAGC+AGG | - | Chr3:72931438-72931457 | MsG0380015640.01.T01:intron | 50.0% | |
| TCAGAGCAGGGTTATGTCCA+TGG | - | Chr3:72931451-72931470 | MsG0380015640.01.T01:intron | 50.0% | |
| TGTAAACGCCGTTTGGTACC+TGG | - | Chr3:72931548-72931567 | MsG0380015640.01.T01:intron | 50.0% | |
| TGTCACGACCAGGTACCAAA+CGG | + | Chr3:72931559-72931578 | None:intergenic | 50.0% | |
| ! | CTTTTCAACCGTCATGGACC+TGG | - | Chr3:72931374-72931393 | MsG0380015640.01.T01:CDS | 50.0% |
| !! | CCAGGAGAAGTTGGTGGATA+TGG | + | Chr3:72931416-72931435 | None:intergenic | 50.0% |
| !! | CCGTTGATTCCAGGAGAAGT+TGG | + | Chr3:72931425-72931444 | None:intergenic | 50.0% |
| AACCGTCATGGACCTGGTGT+TGG | - | Chr3:72931380-72931399 | MsG0380015640.01.T01:intron | 55.0% | |
| AACCTGCAATTCTCCGCGTC+TGG | + | Chr3:72931683-72931702 | None:intergenic | 55.0% | |
| GACCAACACCAGGTCCATGA+CGG | + | Chr3:72931385-72931404 | None:intergenic | 55.0% | |
| TACCAGACGCGGAGAATTGC+AGG | - | Chr3:72931678-72931697 | MsG0380015640.01.T01:intron | 55.0% | |
| GGACCATACGGACCAACACC+AGG | + | Chr3:72931395-72931414 | None:intergenic | 60.0% | |
| !! | GGACCTGGTGTTGGTCCGTA+TGG | - | Chr3:72931389-72931408 | MsG0380015640.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 72931260 | 72932019 | 72931260 | ID=MsG0380015640.01;Name=MsG0380015640.01 |
| Chr3 | mRNA | 72931260 | 72932019 | 72931260 | ID=MsG0380015640.01.T01;Parent=MsG0380015640.01;Name=MsG0380015640.01.T01;_AED=0.34;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|156 |
| Chr3 | exon | 72931688 | 72932019 | 72931688 | ID=MsG0380015640.01.T01:exon:695;Parent=MsG0380015640.01.T01 |
| Chr3 | exon | 72931260 | 72931398 | 72931260 | ID=MsG0380015640.01.T01:exon:694;Parent=MsG0380015640.01.T01 |
| Chr3 | CDS | 72931688 | 72932019 | 72931688 | ID=MsG0380015640.01.T01:cds;Parent=MsG0380015640.01.T01 |
| Chr3 | CDS | 72931260 | 72931398 | 72931260 | ID=MsG0380015640.01.T01:cds;Parent=MsG0380015640.01.T01 |
| Gene Sequence |
| Protein sequence |
