Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040681.01.T01 | XP_027333989.1 | 61.96 | 347 | 131 | 1 | 2 | 348 | 24 | 369 | 1.66E-163 | 471 |
| MsG0780040681.01.T02 | XP_027333989.1 | 61.96 | 347 | 131 | 1 | 2 | 348 | 24 | 369 | 1.66E-163 | 471 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040681.01.T01 | A0A1S2YDS4 | 58.501 | 347 | 143 | 1 | 2 | 348 | 16 | 361 | 7.85e-158 | 456 |
| MsG0780040681.01.T02 | A0A1S2YDS4 | 58.501 | 347 | 143 | 1 | 2 | 348 | 16 | 361 | 7.85e-158 | 456 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048660.01 | MsG0780040681.01 | 0.821602 | 3.699484e-53 | 3.023500e-50 |
| MsG0180002206.01 | MsG0780040681.01 | 0.802184 | 6.328558e-49 | 3.079953e-46 |
| MsG0180004518.01 | MsG0780040681.01 | 0.801460 | 8.910005e-49 | 4.257469e-46 |
| MsG0180004988.01 | MsG0780040681.01 | 0.812046 | 5.162925e-51 | 3.247831e-48 |
| MsG0180005625.01 | MsG0780040681.01 | 0.827579 | 1.446961e-54 | 1.402522e-51 |
| MsG0280007698.01 | MsG0780040681.01 | 0.800679 | 1.287263e-48 | 6.030569e-46 |
| MsG0280008165.01 | MsG0780040681.01 | 0.874965 | 4.471523e-68 | 2.052617e-64 |
| MsG0280008171.01 | MsG0780040681.01 | 0.832096 | 1.148622e-55 | 1.270897e-52 |
| MsG0280011404.01 | MsG0780040681.01 | 0.811986 | 5.319233e-51 | 3.340901e-48 |
| MsG0380015486.01 | MsG0780040681.01 | 0.858418 | 8.351976e-63 | 2.130194e-59 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040681.01.T01 | MTR_3g096050 | 57.925 | 347 | 145 | 1 | 2 | 348 | 13 | 358 | 1.09e-155 | 441 |
| MsG0780040681.01.T01 | MTR_3g096070 | 54.179 | 347 | 135 | 2 | 2 | 348 | 13 | 335 | 2.59e-141 | 404 |
| MsG0780040681.01.T01 | MTR_2g072790 | 51.445 | 346 | 166 | 2 | 3 | 348 | 11 | 354 | 2.23e-136 | 392 |
| MsG0780040681.01.T01 | MTR_3g096050 | 61.134 | 247 | 96 | 0 | 2 | 248 | 13 | 259 | 3.61e-111 | 325 |
| MsG0780040681.01.T01 | MTR_1g036490 | 37.609 | 343 | 195 | 13 | 18 | 348 | 35 | 370 | 5.32e-62 | 202 |
| MsG0780040681.01.T01 | MTR_3g092900 | 34.104 | 346 | 219 | 6 | 3 | 344 | 18 | 358 | 1.39e-61 | 201 |
| MsG0780040681.01.T01 | MTR_1g036510 | 38.012 | 342 | 195 | 10 | 18 | 348 | 49 | 384 | 6.46e-60 | 197 |
| MsG0780040681.01.T01 | MTR_1g036460 | 36.994 | 346 | 201 | 11 | 4 | 339 | 21 | 359 | 2.51e-59 | 196 |
| MsG0780040681.01.T01 | MTR_2g055940 | 35.244 | 349 | 194 | 9 | 2 | 334 | 16 | 348 | 6.21e-59 | 194 |
| MsG0780040681.01.T01 | MTR_7g011990 | 35.756 | 344 | 185 | 10 | 6 | 332 | 26 | 350 | 1.08e-58 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040681.01.T01 | AT3G53140 | 50.142 | 351 | 170 | 2 | 2 | 348 | 10 | 359 | 3.23e-130 | 377 |
| MsG0780040681.01.T01 | AT5G54160 | 37.135 | 342 | 204 | 8 | 3 | 340 | 18 | 352 | 8.98e-67 | 214 |
| MsG0780040681.01.T01 | AT1G51990 | 35.764 | 288 | 176 | 6 | 62 | 344 | 74 | 357 | 3.13e-56 | 187 |
| MsG0780040681.01.T01 | AT1G51990 | 35.417 | 288 | 177 | 6 | 62 | 344 | 74 | 357 | 5.85e-55 | 184 |
| MsG0780040681.01.T01 | AT1G33030 | 32.659 | 346 | 221 | 7 | 9 | 348 | 11 | 350 | 2.62e-50 | 171 |
| MsG0780040681.01.T01 | AT1G77520 | 30.484 | 351 | 228 | 8 | 10 | 348 | 35 | 381 | 1.85e-47 | 165 |
| MsG0780040681.01.T01 | AT5G53810 | 33.043 | 345 | 212 | 12 | 18 | 348 | 39 | 378 | 2.06e-40 | 146 |
| MsG0780040681.01.T01 | AT1G77530 | 32.768 | 354 | 206 | 13 | 15 | 348 | 40 | 381 | 2.56e-36 | 135 |
| MsG0780040681.01.T01 | AT1G21130 | 31.897 | 348 | 222 | 9 | 6 | 344 | 27 | 368 | 2.63e-36 | 135 |
| MsG0780040681.01.T01 | AT1G21100 | 31.609 | 348 | 223 | 9 | 6 | 344 | 27 | 368 | 3.10e-36 | 135 |
Find 46 sgRNAs with CRISPR-Local
Find 475 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCTCGGAAGCCGACAT+AGG | 0.549653 | 7:+81092070 | None:intergenic |
| AAACTTGTTTGGATGTAAGA+AGG | 0.478710 | 7:+81092252 | None:intergenic |
| AAAGTTCTCACTTGCAGAGG+TGG | 0.525549 | 7:-81091936 | MsG0780040681.01.T01:CDS |
| AACACACCAGAAGCTACAGC+TGG | 0.600846 | 7:+81097284 | None:intergenic |
| AACGGCTTACGATATCTTTG+AGG | 0.480985 | 7:-81091984 | MsG0780040681.01.T01:CDS |
| AAGAAGGGACACTCATTAGA+TGG | 0.354636 | 7:+81092155 | None:intergenic |
| AAGGATAACGAAAACTTGTT+TGG | 0.350312 | 7:+81092241 | None:intergenic |
| AAGTTCTCACTTGCAGAGGT+GGG | 0.615623 | 7:-81091935 | MsG0780040681.01.T01:CDS |
| AATAATGTGATGTGCAGCAG+TGG | 0.659838 | 7:-81092365 | MsG0780040681.01.T01:intron |
| ACAACCTGAGCATACGTTCC+AGG | 0.411672 | 7:+81092008 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGAAAAGGAAGCAACAT+AGG | - | Chr7:81093335-81093354 | MsG0780040681.01.T01:intron | 30.0% | |
| AAATTCACACACTTAAAGTG+TGG | - | Chr7:81094482-81094501 | MsG0780040681.01.T01:intron | 30.0% | |
| AAATTCCCAAACATTAGCAA+GGG | - | Chr7:81095632-81095651 | MsG0780040681.01.T01:intron | 30.0% | |
| AACGACTCATAATTTAACAC+GGG | + | Chr7:81094003-81094022 | None:intergenic | 30.0% | |
| AAGGAAGAATAACAAAGTTG+CGG | - | Chr7:81093295-81093314 | MsG0780040681.01.T01:intron | 30.0% | |
| AAGTGTGCAAAACTCATTTA+AGG | + | Chr7:81093469-81093488 | None:intergenic | 30.0% | |
| AATGTAGTACAAGTATGATG+AGG | - | Chr7:81096524-81096543 | MsG0780040681.01.T01:intron | 30.0% | |
| AATTAATATTCCACTTGCAG+TGG | - | Chr7:81096476-81096495 | MsG0780040681.01.T01:intron | 30.0% | |
| AATTATTGCATGTGATTCCT+CGG | - | Chr7:81091725-81091744 | MsG0780040681.01.T01:CDS | 30.0% | |
| ACAACTGATAATAACTAACC+TGG | + | Chr7:81095243-81095262 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 81089757 | 81097357 | 81089757 | ID=MsG0780040681.01;Name=MsG0780040681.01 |
| Chr7 | mRNA | 81089757 | 81097357 | 81089757 | ID=MsG0780040681.01.T01;Parent=MsG0780040681.01;Name=MsG0780040681.01.T01;_AED=0.20;_eAED=0.20;_QI=127|1|1|1|0.75|0.6|5|559|348 |
| Chr7 | exon | 81091871 | 81092270 | 81091871 | ID=MsG0780040681.01.T01:exon:18783;Parent=MsG0780040681.01.T01 |
| Chr7 | exon | 81097289 | 81097357 | 81097289 | ID=MsG0780040681.01.T01:exon:18782;Parent=MsG0780040681.01.T01 |
| Chr7 | five_prime_UTR | 81097289 | 81097357 | 81097289 | ID=MsG0780040681.01.T01:five_prime_utr;Parent=MsG0780040681.01.T01 |
| Chr7 | five_prime_UTR | 81092213 | 81092270 | 81092213 | ID=MsG0780040681.01.T01:five_prime_utr;Parent=MsG0780040681.01.T01 |
| Chr7 | CDS | 81091871 | 81092212 | 81091871 | ID=MsG0780040681.01.T01:cds;Parent=MsG0780040681.01.T01 |
| Chr7 | CDS | 81091410 | 81091746 | 81091410 | ID=MsG0780040681.01.T01:cds;Parent=MsG0780040681.01.T01 |
| Chr7 | CDS | 81090710 | 81090774 | 81090710 | ID=MsG0780040681.01.T01:cds;Parent=MsG0780040681.01.T01 |
| Chr7 | CDS | 81090316 | 81090618 | 81090316 | ID=MsG0780040681.01.T01:cds;Parent=MsG0780040681.01.T01 |
| Chr7 | three_prime_UTR | 81089757 | 81090315 | 81089757 | ID=MsG0780040681.01.T01:three_prime_utr;Parent=MsG0780040681.01.T01 |
| Chr7 | mRNA | 81089757 | 81092394 | 81089757 | ID=MsG0780040681.01.T02;Parent=MsG0780040681.01;Name=MsG0780040681.01.T02;_AED=0.19;_eAED=0.19;_QI=182|1|1|1|1|1|4|559|348 |
| Chr7 | exon | 81091871 | 81092394 | 81091871 | ID=MsG0780040681.01.T02:exon:18787;Parent=MsG0780040681.01.T02 |
| Chr7 | five_prime_UTR | 81092213 | 81092394 | 81092213 | ID=MsG0780040681.01.T02:five_prime_utr;Parent=MsG0780040681.01.T02 |
| Chr7 | CDS | 81091871 | 81092212 | 81091871 | ID=MsG0780040681.01.T02:cds;Parent=MsG0780040681.01.T02 |
| Chr7 | CDS | 81091410 | 81091746 | 81091410 | ID=MsG0780040681.01.T02:cds;Parent=MsG0780040681.01.T02 |
| Chr7 | CDS | 81090710 | 81090774 | 81090710 | ID=MsG0780040681.01.T02:cds;Parent=MsG0780040681.01.T02 |
| Chr7 | CDS | 81090316 | 81090618 | 81090316 | ID=MsG0780040681.01.T02:cds;Parent=MsG0780040681.01.T02 |
| Chr7 | three_prime_UTR | 81089757 | 81090315 | 81089757 | ID=MsG0780040681.01.T02:three_prime_utr;Parent=MsG0780040681.01.T02 |
| Gene Sequence |
>MsG0780040681.01.T02 ATGAGCAAAGCAAGGTTAGCAATAATGAATTTATCCCATCTAATGAGTGTCCCTTCTTCTCTAGTTGCCGTAATTGAACTAGATGTTCCGAATACCATTTTCAACAAAGGAAGCAACCTTCCTATGTCGGCTTCCGAGATTTTGGCAATTGTTCGCCCAAACGGAGGAAGGGAAGCAGATAACCTGGAACGTATGCTCAGGTTGTTAACGGCTTACGATATCTTTGAGGAGCACTTGATGAGTAATGGAGTTAGAAAGTTCTCACTTGCAGAGGTGGGTAAATCTCTTGCTGAAGATGAAGACGGTCTCTCTTATGCCCCTTACATGCTTCAACACCACCAGCATGCGTATATGAGGGCATGGCCTCTCCTTAAGGACGTGGTTGAGGACCCATCCACAGAACCATTCCGAAAGGTGAACGGGGAACCAGCACATGACTTTTACGTAAAATATCGAGAACAAATGAAGTTGTTTAACAAAGCTTTTATTGGAATGACCATTCCATTTTTTAGAGAAGTTTTGGAATCATACGATGGGTTTCGTGGTGTGGAAACTTTAGTGGATGTCGGAGGCAATACGGGCATTTCTTTGCAGATTATAATGGCTAAATTCCCAAACATTAGCAAGGGAATCAACTTTGATCTCCCCGAAATTGTTGCAAGTGCATCCCAACTCCCAGGCATAACCCATGTTGGTGGTGATGCCACTGAGTTTGTTCCTCCTGGAGACGCTATCCTGATAAAGTGGTTGTGTTTGGGTCTGTCAGATGAACAATGTAGTACAAGTATGATGAGGAATTGCCATCGCACTCTTCCGGCGCATGGCAAACTTATAATTTGTGATCCTGTGCTGCCAGAGGTGACGGACAAAAGCACCAGGTCAAAGGCTTTGCTAGGTGGGAATGTCTTCATGATGACCATGTACGGGTCAAAAGCAAAACATAGAACTGAAAAGGAGTTCAAACAGCTGGGTGTGGCTGCTGGTTTTTCGAAGTTAAGTGCTTTCTATGTTGATGCATACATGACATTTTTGGAGTATCACAAGTAG >MsG0780040681.01.T01 ATGAGCAAAGCAAGGTTAGCAATAATGAATTTATCCCATCTAATGAGTGTCCCTTCTTCTCTAGTTGCCGTAATTGAACTAGATGTTCCGAATACCATTTTCAACAAAGGAAGCAACCTTCCTATGTCGGCTTCCGAGATTTTGGCAATTGTTCGCCCAAACGGAGGAAGGGAAGCAGATAACCTGGAACGTATGCTCAGGTTGTTAACGGCTTACGATATCTTTGAGGAGCACTTGATGAGTAATGGAGTTAGAAAGTTCTCACTTGCAGAGGTGGGTAAATCTCTTGCTGAAGATGAAGACGGTCTCTCTTATGCCCCTTACATGCTTCAACACCACCAGCATGCGTATATGAGGGCATGGCCTCTCCTTAAGGACGTGGTTGAGGACCCATCCACAGAACCATTCCGAAAGGTGAACGGGGAACCAGCACATGACTTTTACGTAAAATATCGAGAACAAATGAAGTTGTTTAACAAAGCTTTTATTGGAATGACCATTCCATTTTTTAGAGAAGTTTTGGAATCATACGATGGGTTTCGTGGTGTGGAAACTTTAGTGGATGTCGGAGGCAATACGGGCATTTCTTTGCAGATTATAATGGCTAAATTCCCAAACATTAGCAAGGGAATCAACTTTGATCTCCCCGAAATTGTTGCAAGTGCATCCCAACTCCCAGGCATAACCCATGTTGGTGGTGATGCCACTGAGTTTGTTCCTCCTGGAGACGCTATCCTGATAAAGTGGTTGTGTTTGGGTCTGTCAGATGAACAATGTAGTACAAGTATGATGAGGAATTGCCATCGCACTCTTCCGGCGCATGGCAAACTTATAATTTGTGATCCTGTGCTGCCAGAGGTGACGGACAAAAGCACCAGGTCAAAGGCTTTGCTAGGTGGGAATGTCTTCATGATGACCATGTACGGGTCAAAAGCAAAACATAGAACTGAAAAGGAGTTCAAACAGCTGGGTGTGGCTGCTGGTTTTTCGAAGTTAAGTGCTTTCTATGTTGATGCATACATGACATTTTTGGAGTATCACAAGTAG |
| Protein sequence |
>MsG0780040681.01.T02 MSKARLAIMNLSHLMSVPSSLVAVIELDVPNTIFNKGSNLPMSASEILAIVRPNGGREADNLERMLRLLTAYDIFEEHLMSNGVRKFSLAEVGKSLAEDEDGLSYAPYMLQHHQHAYMRAWPLLKDVVEDPSTEPFRKVNGEPAHDFYVKYREQMKLFNKAFIGMTIPFFREVLESYDGFRGVETLVDVGGNTGISLQIIMAKFPNISKGINFDLPEIVASASQLPGITHVGGDATEFVPPGDAILIKWLCLGLSDEQCSTSMMRNCHRTLPAHGKLIICDPVLPEVTDKSTRSKALLGGNVFMMTMYGSKAKHRTEKEFKQLGVAAGFSKLSAFYVDAYMTFLEYHK* >MsG0780040681.01.T01 MSKARLAIMNLSHLMSVPSSLVAVIELDVPNTIFNKGSNLPMSASEILAIVRPNGGREADNLERMLRLLTAYDIFEEHLMSNGVRKFSLAEVGKSLAEDEDGLSYAPYMLQHHQHAYMRAWPLLKDVVEDPSTEPFRKVNGEPAHDFYVKYREQMKLFNKAFIGMTIPFFREVLESYDGFRGVETLVDVGGNTGISLQIIMAKFPNISKGINFDLPEIVASASQLPGITHVGGDATEFVPPGDAILIKWLCLGLSDEQCSTSMMRNCHRTLPAHGKLIICDPVLPEVTDKSTRSKALLGGNVFMMTMYGSKAKHRTEKEFKQLGVAAGFSKLSAFYVDAYMTFLEYHK* |
