AlfalfaGEDB | MsG0380016061.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016061.01

KEGG: K08176
GO: GO:0016021 GO:0022857 GO:0055085
PFAM: PF00083

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016061.01.T01	XP_003601529.1	85.767	541	3	3	1	467	1	541	0	917

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016061.01.T01	A7KTC5	67.407	540	97	5	1	467	1	534	0	720

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016061.01.T01	B2G3Q0	85.767	541	3	3	1	467	1	541	0.0	917

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380016060.01	MsG0380016061.01	0.863840	1.867976e-64	5.742886e-61
MsG0380016061.01	MsG0480020225.01	0.803166	3.968502e-49	1.980413e-46
MsG0380016061.01	MsG0480022120.01	0.810653	1.035613e-50	6.278563e-48
MsG0380016061.01	MsG0480023250.01	0.814255	1.690943e-51	1.128999e-48
MsG0380016061.01	MsG0580024853.01	0.816686	4.865863e-52	3.471238e-49
MsG0380016061.01	MsG0680030695.01	0.824295	8.723040e-54	7.694305e-51
MsG0380016061.01	MsG0780036291.01	0.805416	1.348970e-49	7.132740e-47
MsG0380016061.01	MsG0780041753.01	0.825210	5.307290e-54	4.806428e-51
MsG0380016061.01	MsG0880045590.01	0.829128	6.121250e-55	6.208172e-52

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0380016061.01.T01	MTR_3g082700	85.767	541	3	3	1	467	1	541	917
MsG0380016061.01.T01	MTR_1g074930	67.970	537	96	4	5	467	4	538	729
MsG0380016061.01.T01	MTR_1g069935	65.835	521	102	3	1	448	1	518	686
MsG0380016061.01.T01	MTR_1g043220	67.816	522	94	3	5	452	4	525	686
MsG0380016061.01.T01	MTR_1g043200	67.754	521	94	3	5	451	4	524	684
MsG0380016061.01.T01	MTR_1g074940	71.368	468	96	3	1	431	1	467	681
MsG0380016061.01.T01	MTR_1g043290	67.433	522	96	3	5	452	4	525	680
MsG0380016061.01.T01	MTR_7g096870	65.121	539	111	4	1	465	1	536	677
MsG0380016061.01.T01	MTR_7g096880	71.795	468	93	2	1	434	1	463	672
MsG0380016061.01.T01	MTR_1g069930	60.075	536	138	4	1	463	1	533	632

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016061.01.T01	AT3G54700	69.216	523	82	4	1	449	1	518	0.0	717
MsG0380016061.01.T01	AT3G54700	69.216	523	82	4	1	449	1	518	0.0	717
MsG0380016061.01.T01	AT2G32830	66.974	542	104	4	1	467	1	542	0.0	716
MsG0380016061.01.T01	AT2G38940	73.077	468	92	1	1	434	1	468	0.0	711
MsG0380016061.01.T01	AT5G43350	66.221	524	103	3	1	451	1	523	0.0	691
MsG0380016061.01.T01	AT5G43360	66.475	522	101	3	1	449	1	521	0.0	691
MsG0380016061.01.T01	AT5G43370	66.859	519	98	3	1	446	1	518	0.0	660
MsG0380016061.01.T01	AT5G43370	66.859	519	98	3	1	446	1	518	0.0	660
MsG0380016061.01.T01	AT5G43340	59.884	516	145	4	1	455	1	515	0.0	621
MsG0380016061.01.T01	AT1G20860	48.319	476	198	8	6	444	3	467	1.01e-145	427

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Find 81 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTTACCTTGCTGTCT+CGG	0.405238	3:+78544547	None:intergenic
AAAGATATTTATAGTAGTAT+CGG	0.503148	3:-78544161	MsG0380016061.01.T01:CDS
AAAGTTTACCTTGCTGTCTC+GGG	0.450939	3:+78544548	None:intergenic
AAATCATTGGAAGAAATGAG+TGG	0.620125	3:-78543595	MsG0380016061.01.T01:CDS
AAGGAGTTTCTTAGAAGACA+CGG	0.654294	3:-78544257	MsG0380016061.01.T01:CDS
AATATCGAGAAGAAACCAAG+TGG	0.702390	3:+78544211	None:intergenic
AATGTATCAGCAGCAGTAAA+TGG	0.399626	3:-78545054	MsG0380016061.01.T01:CDS
ACAACAAAAGTGGTTGAATT+TGG	0.258019	3:+78543858	None:intergenic
ACAACGCTTATAGCACTTTG+TGG	0.467494	3:-78544077	MsG0380016061.01.T01:CDS
ACATAATAGTAGCTGAAAGA+GGG	0.611460	3:+78544822	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCATTGGAAGAAATGAG+TGG	-	Chr3:78545179-78545198	MsG0380016061.01.T01:CDS	30.0%
ACAACAAAAGTGGTTGAATT+TGG	+	Chr3:78544919-78544938	None:intergenic	30.0%
ACATAATAGTAGCTGAAAGA+GGG	+	Chr3:78543955-78543974	None:intergenic	30.0%
ACCTATATAGATAAGTAGCT+CGG	+	Chr3:78544360-78544379	None:intergenic	30.0%
AGAAGCTAATGGAAAATCAT+TGG	-	Chr3:78545166-78545185	MsG0380016061.01.T01:CDS	30.0%
CAAGAAAAAGTAGACAAGAT+AGG	-	Chr3:78544460-78544479	MsG0380016061.01.T01:intron	30.0%
GTATATTCTTCCAAGTAACT+TGG	+	Chr3:78543658-78543677	None:intergenic	30.0%
TAACATTTGGTATGCAGAAA+AGG	+	Chr3:78543635-78543654	None:intergenic	30.0%
AAGGAGTTTCTTAGAAGACA+CGG	-	Chr3:78544517-78544536	MsG0380016061.01.T01:intron	35.0%
AATATCGAGAAGAAACCAAG+TGG	+	Chr3:78544566-78544585	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	78543519	78545277	78543519	ID=MsG0380016061.01;Name=MsG0380016061.01
Chr3	mRNA	78543519	78545277	78543519	ID=MsG0380016061.01.T01;Parent=MsG0380016061.01;Name=MsG0380016061.01.T01;_AED=0.24;_eAED=0.24;_QI=0\|0.33\|0\|1\|1\|1\|4\|0\|467
Chr3	exon	78545013	78545277	78545013	ID=MsG0380016061.01.T01:exon:17485;Parent=MsG0380016061.01.T01
Chr3	exon	78544557	78544911	78544557	ID=MsG0380016061.01.T01:exon:17484;Parent=MsG0380016061.01.T01
Chr3	exon	78543745	78544418	78543745	ID=MsG0380016061.01.T01:exon:17483;Parent=MsG0380016061.01.T01
Chr3	exon	78543519	78543628	78543519	ID=MsG0380016061.01.T01:exon:17482;Parent=MsG0380016061.01.T01
Chr3	CDS	78545013	78545277	78545013	ID=MsG0380016061.01.T01:cds;Parent=MsG0380016061.01.T01
Chr3	CDS	78544557	78544911	78544557	ID=MsG0380016061.01.T01:cds;Parent=MsG0380016061.01.T01
Chr3	CDS	78543745	78544418	78543745	ID=MsG0380016061.01.T01:cds;Parent=MsG0380016061.01.T01
Chr3	CDS	78543519	78543628	78543519	ID=MsG0380016061.01.T01:cds;Parent=MsG0380016061.01.T01

Gene Sequence

>MsG0380016061.01.T01

ATGGCTCGTGAACAACTAGGAGTTCTAACTGCCCTTGATGCAGCCAAAACACAATGGTACCATTTCACAGCCATCATAATTGCTGGTATGGGATTCTTCACAGATGCATATGACCTTTTCTGCATACCAAATGTTACCAAGTTACTTGGAAGAATATACTACACACACCCAGGTGCACTCAAGCCTGGAACTTTGCCTCCAAATGTATCAGCAGCAGTAAATGGTGTTGCTCTTTGTGGCACTCTAGCAGGCCAACTTTTCTTTGCAAAGGGTACAATTGGAACTCTTTGTTTCTTTAGATTTTGGCTAGGTTTTGGTATTGGTGGTGATTACCCTCTTTCAGCTACTATTATGTCTGAATATGCTAACAAAAAGACTCGTGGCGCGTTTATAGCTTCGGTTTTTGCTATGCAAGGGTTTGGAATTTTGGCTGGTGGAATTGTTTCTCTTATAGTTTCAACTGCTTTTGATCATGCTTTTAAGGCTCCGCCTTATAAAGTTGACGCTGCAGCATCACTTGTACCAGAAGCTGATTATGTTTGGAGAATCATTCTCATGTTTGGCGCTGTTCCTGCTGGTTTAACTTACTATTGGCGAATGAAAATGCCCGAGACAGCAAGGTACACTGCACTTGTTGCTAAGAATGCAAAACAAGCAGCGCAGGACATGTCAAGTGTGTTACAGGTTGAAATTGAAGCTGAACAAGAAAAAGTAGACAAGATAGGCGTGCAAGACAAGAACAGTTTTGGTTTGTTTAGTAAGGAGTTTCTTAGAAGACACGGTCTTCATCTGCTTGGAACAACTTCCACTTGGTTTCTTCTCGATATTGCTTATTATAGTTCAAATCTTTTTCAAAAAGATATTTATAGTAGTATCGGTTGGCTTCCACCGGCACAAGATATGAATGCAATTCATGAAGTTTTCAAAGTTGCTAGAGCGACAACGCTTATAGCACTTTGTGGAACTGTTCCGGGATACTGGTTCACGGTGGCTTTTATCGATGTTATTGGTCGTTTTGCAATTCAACTAATGGGATTCTTCTTTATGACTGTGTTCATGTTTGCGCTCGCTATTCCGTATGATCATTGGACTAAAAGAGAAAACAGAATTGGATTTCTTGTTATGTACGCAATGACTTTTTTCTTTGCAAATTTTGGTCCAAATTCAACCACTTTTGTTGTGCCTGCCGAGATTTTCCCGGCTAGGTTGAGGTCTACATGTCATGGTATATCCTCTGCAGCAGGGAAAGCTGGAGCAATTATAGGTGCATTTGGATTCTTGTATGCTTCACAAAAAGCTAATGGAAAATCATTGGAAGAAATGAGTGGAGAGAATGAAGAAGAAGAAACTGATGCTGCTGTTGTTGATCCACAAGCCTCTTCTAATAGGACTGTTCCTGTGTGA

Protein sequence

>MsG0380016061.01.T01

MAREQLGVLTALDAAKTQWYHFTAIIIAGMGFFTDAYDLFCIPNVTKLLGRIYYTHPGALKPGTLPPNVSAAVNGVALCGTLAGQLFFAKGTIGTLCFFRFWLGFGIGGDYPLSATIMSEYANKKTRGAFIASVFAMQGFGILAGGIVSLIVSTAFDHAFKAPPYKVDAAASLVPEADYVWRIILMFGAVPAGLTYYWRMKMPETARYTALVAKNAKQAAQDMSSVLQVEIEAEQEKVDKIGVQDKNSFGLFSKEFLRRHGLHLLGTTSTWFLLDIAYYSSNLFQKDIYSSIGWLPPAQDMNAIHEVFKVARATTLIALCGTVPGYWFTVAFIDVIGRFAIQLMGFFFMTVFMFALAIPYDHWTKRENRIGFLVMYAMTFFFANFGPNSTTFVVPAEIFPARLRSTCHGISSAAGKAGAIIGAFGFLYASQKANGKSLEEMSGENEEEETDAAVVDPQASSNRTVPV*