AlfalfaGEDB | MsG0780036291.01

M. sativa cultivar ZhongmuNo.1 / MsG0780036291.01

KEGG: K02183
GO: GO:0005509
PFAM: PF13499

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036291.01.T01	XP_003621252.1	91.584	202	17	0	1	202	1	202	9.35E-119	345

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036291.01.T01	Q93Z27	44.651	215	95	6	1	202	1	204	9.64E-47	155

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036291.01.T01	G7L207	91.584	202	17	0	1	202	1	202	4.46e-119	345

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0780036291.01	0.809081	2.256890e-50	1.312509e-47
MsG0080048656.01	MsG0780036291.01	0.829976	3.808576e-55	3.959413e-52
MsG0180000157.01	MsG0780036291.01	0.828217	1.016442e-54	1.003840e-51
MsG0180000222.01	MsG0780036291.01	0.813006	3.184723e-51	2.055475e-48
MsG0180000267.01	MsG0780036291.01	0.817833	2.686829e-52	1.978024e-49
MsG0180000673.01	MsG0780036291.01	0.845435	4.069310e-59	6.780679e-56
MsG0180001043.01	MsG0780036291.01	0.812389	4.345695e-51	2.759554e-48
MsG0180003088.01	MsG0780036291.01	0.804256	2.356876e-49	1.209487e-46
MsG0180003241.01	MsG0780036291.01	0.837330	5.538792e-57	7.170786e-54
MsG0180004116.01	MsG0780036291.01	0.802496	5.456632e-49	2.676854e-46
MsG0180004258.01	MsG0780036291.01	0.802580	5.245453e-49	2.578709e-46
MsG0180004388.01	MsG0780036291.01	0.805873	1.081709e-49	5.787301e-47
MsG0180005505.01	MsG0780036291.01	0.817993	2.472095e-52	1.827911e-49
MsG0180005869.01	MsG0780036291.01	0.806999	6.261602e-50	3.447990e-47
MsG0380011754.01	MsG0780036291.01	0.848609	5.497023e-60	1.014310e-56
MsG0380013047.01	MsG0780036291.01	0.812148	4.904140e-51	3.093927e-48
MsG0380013436.01	MsG0780036291.01	0.803422	3.512928e-49	1.764624e-46
MsG0680031459.01	MsG0780036291.01	0.805841	1.098482e-49	5.872263e-47
MsG0680031538.01	MsG0780036291.01	0.813858	2.068967e-51	1.366561e-48
MsG0680031694.01	MsG0780036291.01	0.814634	1.394257e-51	9.405098e-49
MsG0680032641.01	MsG0780036291.01	0.827860	1.238961e-54	1.210934e-51
MsG0680034450.01	MsG0780036291.01	0.829884	4.010305e-55	4.158511e-52
MsG0680034672.01	MsG0780036291.01	0.816805	4.576680e-52	3.275615e-49
MsG0480019871.01	MsG0780036291.01	0.813363	2.659110e-51	1.733027e-48
MsG0480020225.01	MsG0780036291.01	0.800391	1.473667e-48	6.853602e-46
MsG0480020445.01	MsG0780036291.01	0.807141	5.842996e-50	3.229350e-47
MsG0480020839.01	MsG0780036291.01	0.804017	2.642966e-49	1.348109e-46
MsG0480021021.01	MsG0780036291.01	0.809750	1.621252e-50	9.598740e-48
MsG0480022274.01	MsG0780036291.01	0.801986	6.948810e-49	3.364854e-46
MsG0480023280.01	MsG0780036291.01	0.818273	2.135994e-52	1.591530e-49
MsG0480023417.01	MsG0780036291.01	0.833896	4.097078e-56	4.783990e-53
MsG0480023517.01	MsG0780036291.01	0.804922	1.711857e-49	8.938098e-47
MsG0480023903.01	MsG0780036291.01	0.845933	2.980681e-59	5.047725e-56
MsG0580024073.01	MsG0780036291.01	0.808517	2.979819e-50	1.707301e-47
MsG0580024853.01	MsG0780036291.01	0.811855	5.681436e-51	3.555775e-48
MsG0580025278.01	MsG0780036291.01	0.844106	9.281857e-59	1.483070e-55
MsG0580025453.01	MsG0780036291.01	0.834909	2.281601e-56	2.746152e-53
MsG0580025769.01	MsG0780036291.01	0.820353	7.175040e-53	5.663873e-50
MsG0380015931.01	MsG0780036291.01	0.807933	3.966801e-50	2.238100e-47
MsG0380015986.01	MsG0780036291.01	0.810811	9.573623e-51	5.828594e-48
MsG0380016061.01	MsG0780036291.01	0.805416	1.348970e-49	7.132740e-47
MsG0380017226.01	MsG0780036291.01	0.821091	4.853804e-53	3.911134e-50
MsG0380017286.01	MsG0780036291.01	0.864121	1.527438e-64	4.742154e-61
MsG0480018321.01	MsG0780036291.01	0.800173	1.631808e-48	7.546995e-46
MsG0580027034.01	MsG0780036291.01	0.821756	3.409254e-53	2.798484e-50
MsG0580027129.01	MsG0780036291.01	0.824081	9.791596e-54	8.585201e-51
MsG0580027401.01	MsG0780036291.01	0.860103	2.608226e-63	7.048815e-60
MsG0580028416.01	MsG0780036291.01	0.802382	5.761104e-49	2.818214e-46
MsG0580028453.01	MsG0780036291.01	0.815820	7.599825e-52	5.294729e-49
MsG0580029758.01	MsG0780036291.01	0.816015	6.873968e-52	4.814945e-49
MsG0680030629.01	MsG0780036291.01	0.830092	3.567650e-55	3.721134e-52
MsG0280006581.01	MsG0780036291.01	0.805741	1.152832e-49	6.147238e-47
MsG0280007258.01	MsG0780036291.01	0.857749	1.320514e-62	3.293365e-59
MsG0280007272.01	MsG0780036291.01	0.808997	2.352681e-50	1.365090e-47
MsG0280007439.01	MsG0780036291.01	0.810758	9.830666e-51	5.976870e-48
MsG0280007481.01	MsG0780036291.01	0.811411	7.097110e-51	4.390127e-48
MsG0280007714.01	MsG0780036291.01	0.830131	3.490592e-55	3.645156e-52
MsG0280008220.01	MsG0780036291.01	0.870667	1.226765e-66	4.810838e-63
MsG0280010378.01	MsG0780036291.01	0.847508	1.106097e-59	1.970413e-56
MsG0780036291.01	MsG0780038444.01	0.835828	1.336972e-56	1.654107e-53
MsG0780036291.01	MsG0780038495.01	0.810283	1.245178e-50	7.474721e-48
MsG0780036291.01	MsG0780038829.01	0.817837	2.680975e-52	1.973937e-49
MsG0780036291.01	MsG0780039875.01	0.815640	8.333293e-52	5.777688e-49
MsG0780036291.01	MsG0780040679.01	0.851052	1.140647e-60	2.279723e-57
MsG0780036291.01	MsG0780040726.01	0.825611	4.265781e-54	3.907375e-51
MsG0780036291.01	MsG0780041196.01	0.803382	3.581157e-49	1.796937e-46
MsG0780036291.01	MsG0880042118.01	0.801607	8.315000e-49	3.987757e-46
MsG0780036291.01	MsG0880042611.01	0.830529	2.790483e-55	2.947648e-52
MsG0780036291.01	MsG0880042843.01	0.809759	1.614424e-50	9.560414e-48
MsG0780036291.01	MsG0880043208.01	0.804839	1.781798e-49	9.283163e-47
MsG0780036291.01	MsG0880043288.01	0.810351	1.203767e-50	7.239334e-48
MsG0780036291.01	MsG0880045518.01	0.806614	7.550355e-50	4.116537e-47
MsG0780036291.01	MsG0880045803.01	0.833236	5.989982e-56	6.856483e-53
MsG0780036291.01	MsG0880046244.01	0.800830	1.198898e-48	5.638180e-46
MsG0780036291.01	MsG0880046440.01	0.850384	1.758253e-60	3.438074e-57
MsG0780036291.01	MsG0880046719.01	0.831076	2.048720e-55	2.199514e-52
MsG0780036291.01	MsG0880046731.01	0.872610	2.785998e-67	1.173132e-63

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036291.01.T01	MTR_7g011010	91.584	202	17	0	1	202	1	202	1.13e-122	345
MsG0780036291.01.T01	MTR_5g025690	84.466	206	22	2	1	202	1	200	1.97e-116	329
MsG0780036291.01.T01	MTR_4g127560	68.852	183	55	2	22	202	16	198	3.66e-76	227
MsG0780036291.01.T01	MTR_2g023310	84.615	52	8	0	84	135	46	97	6.73e-23	88.6
MsG0780036291.01.T01	MTR_4g082050	48.148	108	53	2	91	198	35	139	1.88e-21	86.3
MsG0780036291.01.T01	MTR_7g074240	52.308	65	31	0	133	197	106	170	2.48e-14	68.6
MsG0780036291.01.T01	MTR_2g098890	28.061	196	116	6	25	198	11	203	1.96e-13	66.6
MsG0780036291.01.T01	MTR_4g115170	44.595	74	41	0	126	199	145	218	1.29e-12	64.7
MsG0780036291.01.T01	MTR_3g109320	37.500	120	66	2	86	197	60	178	1.03e-11	61.2
MsG0780036291.01.T01	MTR_7g089760	47.541	61	32	0	138	198	82	142	1.20e-11	60.5
MsG0780036291.01.T01	MTR_4g067270	43.284	67	38	0	133	199	77	143	2.43e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780036291.01.T01	AT5G39670	44.651	215	95	6	1	202	1	204	9.83e-48	155
MsG0780036291.01.T01	AT3G29000	62.385	109	39	1	91	199	85	191	9.35e-40	135
MsG0780036291.01.T01	AT4G20780	57.746	71	27	1	127	197	116	183	4.99e-17	75.9
MsG0780036291.01.T01	AT3G47480	56.061	66	28	1	133	198	115	179	2.41e-16	73.9
MsG0780036291.01.T01	AT5G44460	52.113	71	31	1	129	199	109	176	4.02e-16	73.2
MsG0780036291.01.T01	AT2G43290	33.077	130	68	2	87	198	79	207	4.13e-15	71.2
MsG0780036291.01.T01	AT3G59440	28.283	198	103	6	25	199	7	188	3.87e-14	68.2
MsG0780036291.01.T01	AT1G05990	47.692	65	34	0	135	199	80	144	1.54e-13	65.9
MsG0780036291.01.T01	AT4G03290	46.875	64	34	0	135	198	82	145	1.30e-12	63.2
MsG0780036291.01.T01	AT3G07490	45.161	62	34	0	138	199	82	143	5.35e-12	61.6
MsG0780036291.01.T01	AT1G21550	35.135	111	65	2	87	193	41	148	9.66e-11	58.2
MsG0780036291.01.T01	AT4G12860	46.667	60	32	0	138	197	82	141	9.92e-11	58.2

Find 22 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAAAGAGTTATGTGTATTT+TGG	0.154565	7:+6073012	MsG0780036291.01.T01:CDS
TGAAGCGGTGATGGCGAAAA+TGG	0.258329	7:+6072829	MsG0780036291.01.T01:CDS
GAGAAAGTGGAGAGATTAAG+AGG	0.304916	7:+6072798	MsG0780036291.01.T01:CDS
GAAAGCAACTCAACTTTGTT+TGG	0.330887	7:+6072605	MsG0780036291.01.T01:CDS
CTTCTCTGCCAACTTCGTTC+CGG	0.355688	7:+6072701	MsG0780036291.01.T01:CDS
TGATGAGAATGAGCCAAGTT+TGG	0.401446	7:+6072922	MsG0780036291.01.T01:CDS
ATCTTCACTTCCTCCAAACT+TGG	0.413419	7:-6072935	None:intergenic
TTCCGGTGACTTAGAAGTAA+AGG	0.466313	7:+6072718	MsG0780036291.01.T01:CDS
CGGAACGAAGTTGGCAGAGA+AGG	0.482954	7:-6072700	None:intergenic
TCCGGTGACTTAGAAGTAAA+GGG	0.483748	7:+6072719	MsG0780036291.01.T01:CDS
AAGTTTGGAGGAAGTGAAGA+TGG	0.496634	7:+6072937	MsG0780036291.01.T01:CDS
TCCCTTTACTTCTAAGTCAC+CGG	0.500276	7:-6072720	None:intergenic
GAAGCGGTGATGGCGAAAAT+GGG	0.504720	7:+6072830	MsG0780036291.01.T01:CDS
TCGAATGTAAGCGGAGAAAG+TGG	0.508948	7:+6072785	MsG0780036291.01.T01:CDS
TGAGAATGAGCCAAGTTTGG+AGG	0.510803	7:+6072925	MsG0780036291.01.T01:CDS
GGATGAAGTTGAAGCGGTGA+TGG	0.511527	7:+6072820	MsG0780036291.01.T01:CDS
CTAAGTCACCGGAACGAAGT+TGG	0.521372	7:-6072709	None:intergenic
CTGCAATAAGATAAACAAGT+TGG	0.540643	7:-6072559	None:intergenic
AGAAAGTGGAGAGATTAAGA+GGG	0.546580	7:+6072799	MsG0780036291.01.T01:CDS
TAAGAGGGATGAAGTTGAAG+CGG	0.562793	7:+6072814	MsG0780036291.01.T01:CDS
ACTTGTTTATCTTATTGCAG+AGG	0.590641	7:+6072562	MsG0780036291.01.T01:CDS
AGCAGAGACTCGAATGTAAG+CGG	0.610274	7:+6072776	MsG0780036291.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTGAATTTGTAAAAATTA+TGG	+	Chr7:6073117-6073136	MsG0780036291.01.T01:CDS	15.0%
!!!	AATTTTGATGAAAATCAAGA+TGG	+	Chr7:6073082-6073101	MsG0780036291.01.T01:CDS	20.0%
!	GATGAACTTGAAGAAAAATA+TGG	+	Chr7:6072875-6072894	MsG0780036291.01.T01:CDS	25.0%
!	TGAAGAAAAATATGGTTCAA+AGG	+	Chr7:6072883-6072902	MsG0780036291.01.T01:CDS	25.0%
!!	CAAAGAGTTATGTGTATTTT+GGG	+	Chr7:6073013-6073032	MsG0780036291.01.T01:CDS	25.0%
	ACTTGTTTATCTTATTGCAG+AGG	+	Chr7:6072562-6072581	MsG0780036291.01.T01:CDS	30.0%
	CTGCAATAAGATAAACAAGT+TGG	-	Chr7:6072562-6072581	None:intergenic	30.0%
!	GCAAAGAGTTATGTGTATTT+TGG	+	Chr7:6073012-6073031	MsG0780036291.01.T01:CDS	30.0%
!	GTTTTTGATGAGAACAAAGA+TGG	+	Chr7:6072968-6072987	MsG0780036291.01.T01:CDS	30.0%
!	TTAAGTTCTTCTCGAGTTTT+TGG	+	Chr7:6072672-6072691	MsG0780036291.01.T01:CDS	30.0%
!!!	TATGTGTATTTTGGGATTGA+AGG	+	Chr7:6073021-6073040	MsG0780036291.01.T01:CDS	30.0%
	AGAAAGTGGAGAGATTAAGA+GGG	+	Chr7:6072799-6072818	MsG0780036291.01.T01:CDS	35.0%
	GAAAGCAACTCAACTTTGTT+TGG	+	Chr7:6072605-6072624	MsG0780036291.01.T01:CDS	35.0%
!!!	TGTATTTTGGGATTGAAGGA+AGG	+	Chr7:6073025-6073044	MsG0780036291.01.T01:CDS	35.0%
	AAGTTTGGAGGAAGTGAAGA+TGG	+	Chr7:6072937-6072956	MsG0780036291.01.T01:CDS	40.0%
	ATCTTCACTTCCTCCAAACT+TGG	-	Chr7:6072938-6072957	None:intergenic	40.0%
	GAGAAAGTGGAGAGATTAAG+AGG	+	Chr7:6072798-6072817	MsG0780036291.01.T01:CDS	40.0%
	TAAGAGGGATGAAGTTGAAG+CGG	+	Chr7:6072814-6072833	MsG0780036291.01.T01:CDS	40.0%
	TCCCTTTACTTCTAAGTCAC+CGG	-	Chr7:6072723-6072742	None:intergenic	40.0%
	TCCGGTGACTTAGAAGTAAA+GGG	+	Chr7:6072719-6072738	MsG0780036291.01.T01:CDS	40.0%
	TGATGAGAATGAGCCAAGTT+TGG	+	Chr7:6072922-6072941	MsG0780036291.01.T01:CDS	40.0%
	TTCCGGTGACTTAGAAGTAA+AGG	+	Chr7:6072718-6072737	MsG0780036291.01.T01:CDS	40.0%
	AGCAGAGACTCGAATGTAAG+CGG	+	Chr7:6072776-6072795	MsG0780036291.01.T01:CDS	45.0%
	TCGAATGTAAGCGGAGAAAG+TGG	+	Chr7:6072785-6072804	MsG0780036291.01.T01:CDS	45.0%
	TGAGAATGAGCCAAGTTTGG+AGG	+	Chr7:6072925-6072944	MsG0780036291.01.T01:CDS	45.0%
	CTAAGTCACCGGAACGAAGT+TGG	-	Chr7:6072712-6072731	None:intergenic	50.0%
	CTTCTCTGCCAACTTCGTTC+CGG	+	Chr7:6072701-6072720	MsG0780036291.01.T01:CDS	50.0%
	GGATGAAGTTGAAGCGGTGA+TGG	+	Chr7:6072820-6072839	MsG0780036291.01.T01:CDS	50.0%
!	GAAGCGGTGATGGCGAAAAT+GGG	+	Chr7:6072830-6072849	MsG0780036291.01.T01:CDS	50.0%
!	TGAAGCGGTGATGGCGAAAA+TGG	+	Chr7:6072829-6072848	MsG0780036291.01.T01:CDS	50.0%
	CGGAACGAAGTTGGCAGAGA+AGG	-	Chr7:6072703-6072722	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	6072548	6073156	6072548	ID=MsG0780036291.01;Name=MsG0780036291.01
Chr7	mRNA	6072548	6073156	6072548	ID=MsG0780036291.01.T01;Parent=MsG0780036291.01;Name=MsG0780036291.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|202
Chr7	exon	6072548	6073156	6072548	ID=MsG0780036291.01.T01:exon:3255;Parent=MsG0780036291.01.T01
Chr7	CDS	6072548	6073156	6072548	ID=MsG0780036291.01.T01:cds;Parent=MsG0780036291.01.T01

Gene Sequence

>MsG0780036291.01.T01

ATGGTTTTTCGCCAACTTGTTTATCTTATTGCAGAGGAGAACACATTACAGAATGATGAAAGCAACTCAACTTTGTTTGGTTTGATAGATTTTTTCATGTATTCAACATTATTCATTAGAATACTTAAGTTCTTCTCGAGTTTTTGGTTTTTCCTTCTCTGCCAACTTCGTTCCGGTGACTTAGAAGTAAAGGGAGAGAAGCAGTTATCAGAAACTGAATCGAACGTTAGCAGAGACTCGAATGTAAGCGGAGAAAGTGGAGAGATTAAGAGGGATGAAGTTGAAGCGGTGATGGCGAAAATGGGAATTTTTTGCAGCTCAGAAAGTGATGAACTTGAAGAAAAATATGGTTCAAAGGAACTTTCTGAATTGTTTGATGAGAATGAGCCAAGTTTGGAGGAAGTGAAGATGGCTTTCGATGTTTTTGATGAGAACAAAGATGGATTTATTGATGCAATTGAGTTGCAAAGAGTTATGTGTATTTTGGGATTGAAGGAAGGTTCAGAAGTTAAGAATTGTCAGAAAATGATCAAGAATTTTGATGAAAATCAAGATGGAAGAATTGATTTCATTGAATTTGTAAAAATTATGGAAAACAGATTGTGTTGA

Protein sequence

>MsG0780036291.01.T01

MVFRQLVYLIAEENTLQNDESNSTLFGLIDFFMYSTLFIRILKFFSSFWFFLLCQLRSGDLEVKGEKQLSETESNVSRDSNVSGESGEIKRDEVEAVMAKMGIFCSSESDELEEKYGSKELSELFDENEPSLEEVKMAFDVFDENKDGFIDAIELQRVMCILGLKEGSEVKNCQKMIKNFDENQDGRIDFIEFVKIMENRLC*